163,404 results

1. pC0050-CMV-dPspCas13b-longlinker-ADAR2DD(wt)

   Plasmid

   #103866

   Purpose

   dPspCas13b-ADAR2DD(wt) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Longer linker than pC0039.

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Inserts

   PspCas13b
   huADAR2DD (ADARB1 Human)
   

   Use

   CRISPR

   Tags

   GSGGGGS and HIV NES

   Expression

   Mammalian

   Mutation

   Changed histidne 133 to alanine and histidine 105…

   Promoter

   Available since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-PspCas13b-gRNA-Actb1216

   Plasmid

   #155368

   Purpose

   PspCas13b guide RNA positioned 8bp 5' of Actb 1216 for targeted m6A RNA methylation

   Depositor

   David Liu

   Article

   Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

   Insert

   PspCas13b gRNA targeting Actb 1216
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. KW54_HART_ODC1

   Plasmid

   #207398

   Purpose

   Lentiviral plasmid containing DDX3X reporter HART for ODC1 5UTR

   Depositor

   Stephen Floor

   Article

   Wilkins et al RNA. 2024 Jul 16;30(8):1041-1057. doi: 1

   Insert

   eGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSCrhaB2

   Plasmid

   #113634

   Purpose

   An expression vector containing a rhamnose-inducible promoter to regulate gene expression in Burkholderia cenocepacia

   Depositor

   Miguel Valvano

   Article

   Cardona et al Plasmid. 2005 Nov;54(3):219-28. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PrhaB

   Available since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. fabp10a:lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR2.0; cmlc2:EGFP

   Plasmid

   #230075

   Purpose

   Partial ablation using NTR2 and subsequent lineage tracing of regenerating hepatocytes in zebrafish.

   Depositor

   Sumeet Pal Singh

   Article

   Hovhannisyan et al bioRxiv 2025.05.23.655316

   Insert

   lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR2.0
   

   Use

   Cre/Lox; Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   fabp10a

   Available since

   May 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTXTL-T7max-FLuc

   Plasmid

   #188311

   Purpose

   Expression of Firefly luciferase via a T7 promoter in TXTL

   Depositor

   Kate Adamala

   Article

   Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

   Insert

   Firefly luciferase
   

   Use

   Luciferase and Synthetic Biology

   Tags

   8x His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7Max

   Available since

   Aug. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHM066

   Plasmid

   #225524

   Purpose

   Encodes Wnt-responsive destablized iRFP

   Depositor

   Jared Toettcher

   Article

   McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

   Insert

   iRFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. GCH1_pLX307

   Plasmid

   #98336

   Purpose

   Lentiviral expression of GCH1

   Depositor

   William Hahn, Sefi Rosenbluh

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   GCH1 (GCH1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   E1Fa

   Available since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLQ8069 - ACTB GFP-P2A + RAB11A mCherry-P2A CLIP Donor

   Plasmid

   #202689

   Purpose

   CLIP donor template to generate 2 knock-ins: GFP into ACTB, and mCherry in RAB11A.

   Depositor

   Stanley Qi

   Article

   Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

   Inserts

   CopGFP
   mCherry
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLQ8079 - IL2RG GFP-P2A CLIP Donor

    Plasmid

    #202690

    Purpose

    CLIP donor template for integrating GFP into IL2RG locus.

    Depositor

    Stanley Qi

    Article

    Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

    Insert

    CopGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. KW57_HART_RAC1

    Plasmid

    #207399

    Purpose

    Lentiviral plasmid containing DDX3X reporter HART for RAC1 5UTR

    Depositor

    Stephen Floor

    Article

    Wilkins et al RNA. 2024 Jul 16;30(8):1041-1057. doi: 1

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. lentiGuide-Puro

    Plasmid

    #52963

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Has Service

    Cloning Grade DNA

    Inserts

    S. pyogenes sgRNA cassette
    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1-a and hU6

    Available since

    May 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV2/8

    Plasmid

    #112864

    Purpose

    AAV packaging plasmid expressing Rep/Cap genes

    Depositor

    James M. Wilson

    Insert

    Rep2/Cap8
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Truncated P5

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCL-Eco

    Plasmid

    #12371

    Depositor

    Inder Verma

    Article

    Naviaux et al J Virol. 1996 Aug . 70(8):5701-5.

    Insert

    gag/pol/env
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVX-TetOne-Puro-GFP

    Plasmid

    #171123

    Purpose

    Doxycycline inducible expression of GFP

    Depositor

    Jason Sheltzer

    Article

    Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLJM1-EGFP

    Plasmid

    #19319

    Purpose

    3rd gen lentiviral vector for EGFP fusion; PGK driven puromycin

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP

    Plasmid

    #102796

    Purpose

    Retrovirus for delivery of one sgRNA (empty, bbsI)

    Depositor

    Sarah Teichmann

    Article

    Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

    Insert

    U6 sgRNA
    

    Use

    CRISPR and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pgRNA-bacteria

    Plasmid

    #44251

    Purpose

    Expression of customizable guide RNA (gRNA) for bacterial gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    guide RNA (bacteria)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119 (SpeI)

    Available since

    April 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pU6-tevopreq1-GG-acceptor

    Plasmid

    #174038

    Purpose

    Prime editing in mammalian cells

    Depositor

    David Liu

    Article

    Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-PEmax

    Plasmid

    #174820

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PEmax
    

    Use

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pU6-pegRNA-GG-acceptor

    Plasmid

    #132777

    Purpose

    Prime editing in mammalian cells

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    Exchangeable cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    U6

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. lentiCRISPR v2-Blast

    Plasmid

    #83480

    Purpose

    Mammalian expression of Cas9 and sgRNA scaffold

    Depositor

    Mohan Babu

    Insert

    Blasticidin S deaminase
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Replaced puromycin N-acetyltransferase on the ori…

    Promoter

    EF-1α

    Available since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCRIS-PITChv2-Puro-dTAG (BRD4)

    Plasmid

    #91793

    Purpose

    PITCh dTAG donor vector for Puro-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.

    Depositor

    Behnam Nabet, James Bradner

    Article

    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

    Insert

    Puro-P2A-2xHA-FKBP_F36V
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Phenylalanine 36 to valine in FKBP12

    Promoter

    Promotorless

    Available since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. PB-TRE-dCas9-VPR

    Plasmid

    #63800

    Purpose

    SP-dCas9-VPR with doxycycline-inducible expression

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

    Insert

    dCas9-VPR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    VPR (VP64-p65-Rta)

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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25. pTn Donor

    Plasmid

    #236211

    Purpose

    Arabinose-inducible Tn5 InducTn-seq plasmid

    Depositor

    Matthew Waldor

    Article

    Basta et al Nat Microbiol. 2025 Mar 27. doi: 10.1038

    Insert

    None
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. eeBxb1

    Plasmid

    #222339

    Purpose

    Plasmid for expressing evolved and engineered Bxb1 in the mammalian cells

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    Codon-optimized evolved and engineered Bxb1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLV-mCherry

    Plasmid

    #36084

    Purpose

    3rd generation lentiviral plasmid expressing mCherry

    Depositor

    Pantelis Tsoulfas

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. M50 Super 8x TOPFlash

    Plasmid

    #12456

    Purpose

    Beta-catenin reporter. TCF/LEF sites upstream of a luciferase reporter.

    Depositor

    Randall Moon

    Article

    Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

    Insert

    TCF/LEF binding sites (Ctnnb1 )
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. M51 Super 8x FOPFlash (TOPFlash mutant)

    Plasmid

    #12457

    Purpose

    Control for beta-catenin reporter. Contains mutated TCF/LEF binding sites upstream of a luciferase reporter.

    Depositor

    Randall Moon

    Article

    Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

    Insert

    mutant TCF/LEF binding sites (Ctnnb1 )
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    mutant TCF/LEF binding sites

    Promoter

    Available since

    March 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLenti-pHluorin_M153R-CD63-mScarlet

    Plasmid

    #172118

    Purpose

    Dual-color fluorescent reporter for CD63-positive exosome secretion and uptake

    Depositor

    Alissa Weaver

    Article

    Sung et al Nat Commun. 2020 Apr 29;11(1):2092. doi:

    Insert

    CD63 (CD63 Human)
    

    Use

    Lentiviral

    Tags

    mScarlet and pHluorin_M153R in a small extracellu…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge