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177,145 results

Showing: 1001 - 1050 of 177145 results

  1. pAAV-hSyn-hM4D(Gi)-mCherry

    Plasmid
    #50475
    Purpose
    Gi-coupled hM4D DREADD fused with mCherry under the control of human synapsin promoter.
    Depositor
    Bryan Roth
    Has service
    AAV1, AAV2, AAV5, AAV8, AAV9, and AAV Retrograde
    Insert
    hM4D(Gi)-mCherry
    Use
    AAV
    Tags
    mCherry
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    human Synapsin 1
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    118 Articles
    50475-plasmid-map-sequence-id-171072

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  2. pHIV-Luciferase

    Plasmid
    #21375
    Purpose
    self-inactivating 3rd generation lentiviral plasmid for co-expression of your gene of interest and Luciferase
    Depositor
    Bryan Welm
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available since
    June 19, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    32 Articles
    21375-plasmid-map-sequence-id-357945

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  6. pU6-sgRosa26-1_CBh-Cas9-T2A-BFP

    Plasmid
    #64216
    Purpose
    Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked to BFP via a T2A peptide
    Depositor
    Ralf Kuehn
    Publication
    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    sgRNA targeting ROSA26-1
    Use
    CRISPR
    Tags
    3xFLAG, NLS, and T2A-BFP
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available since
    May 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    11 Articles
    64216-plasmid-map-sequence-id-182615

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  7. CMV-GEM-GECO1

    Plasmid
    #32442
    Purpose
    Mammalian expression of blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imaging
    Depositor
    Robert Campbell
    Publication
    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GEM-GECO1
    Expression
    Mammalian
    Mutation
    GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…
    Promoter
    CMV
    Available since
    Sept. 29, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    32442-plasmid-map-sequence-id-117095

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  8. pAAV-Ef1a-DIO eNpHR 3.0-EYFP

    Plasmid
    #26966
    Purpose
    AAV expression of eNpHR 3.0 fused to EYFP driven by EF1a promoter for optogenetic inhibition
    Depositor
    Karl Deisseroth
    Publication
    Gradinaru et al Cell. 2010 Apr 2. 141(1):154-65.
    Has service
    AAV1, AAV5, AAV9, and AAV Retrograde
    Insert
    eNpHR 3.0
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    ER export at 3' end of gene. Trafficking sig…
    Promoter
    EF1alpha
    Available since
    Jan. 27, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    89 Articles
    26966-plasmid-map-sequence-id-200278

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  12. pDest-565

    Plasmid
    #11520
    Purpose
    Gateway destination vector for bacterial expression; His,GST tag
    Depositor
    Dominic Esposito
    Type
    Empty backbone
    Use
    Gateway Cloning
    Tags
    6xHis and GST
    Expression
    Bacterial
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    May 24, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    9 Articles
    11520-plasmid-map-sequence-id-324017

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  15. pLVX-EF1alpha-SARS-CoV-2-N-2xStrep-IRES-Puro

    Plasmid
    #141391
    Purpose
    Lentiviral expression of SARS-CoV-2 nucleocapsid (N) protein; transient expression and generate lentivirus
    Depositor
    Nevan Krogan
    Publication
    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:
    Insert
    SARS-CoV-2-N (N SARS-CoV-2)
    Use
    Lentiviral
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    EF1alpha
    Available since
    April 8, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    28 Articles
    141391-plasmid-map-sequence-id-288088

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  20. pdCas9

    Plasmid
    #46569
    Purpose
    Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.
    Depositor
    Luciano Marraffini
    Publication
    Bikard et al Nucleic Acids Res. 2013 Jun 12.
    Inserts
    tracrRNA
    dcas9
    CRISPR array
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Available since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    18 Articles
    46569-plasmid-map-sequence-id-364492

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  24. pUCM-AAVS1-TO-hNGN2

    Plasmid
    #105840
    Purpose
    Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron
    Depositor
    Michael Ward
    Publication
    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    mCherry
    rtTA3G
    Use
    CRISPR and TALEN
    Promoter
    CAG, EF-1alpha, and TRE3G
    Available since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    16 Articles
    105840-plasmid-map-sequence-id-472335

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  34. pCDH-EF1-Luc2-P2A-tdTomato

    Plasmid
    #72486
    Purpose
    Express Luc2 and tdTomato under EF1 promoter
    Depositor
    Kazuhiro Oka
    Insert
    Luc2-P2A-tdTomato
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1
    Available since
    Jan. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    30 Articles
    72486-plasmid-map-sequence-id-418875

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  35. tdTomato-C1

    Plasmid
    #54653
    Purpose
    Localization: C1 Cloning Vector, Excitation: 554, Emission: 581
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    tdTomato
    Expression
    Mammalian
    Available since
    June 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    25 Articles
    54653-plasmid-map-sequence-id-450928

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  41. pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

    Plasmid
    #42337
    Purpose
    Dual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.
    Depositor
    Feng Zhang
    Publication
    Cong et al Science. 2013 Jan 3.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. pyogenes Cas9
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Available since
    Feb. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    42337-plasmid-map-sequence-id-57166

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  44. pUltra-hot

    Plasmid
    #24130
    Purpose
    3rd generation Lentiviral vector for bi-cistronic expression of mCherry and the gene of interest
    Depositor
    Malcolm Moore
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Available since
    Feb. 26, 2010
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    50 Articles
    24130-plasmid-map-sequence-id-247629

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  47. pUCmini-iCAP-AAV.CAP-B10

    Plasmid
    #175004
    Purpose
    non-standard AAV2 rep-AAV.CAP-B10 cap plasmid with AAV cap expression controlled by a tTA-TRE amplifcation system
    Depositor
    Viviana Gradinaru
    Publication
    Goertsen et al Nat Neurosci. 2022 Jan;25(1):106-115. do
    Insert
    Synthetic construct isolate AAV.CAP-B10 VP1 gene
    Use
    AAV
    Expression
    Mammalian
    Mutation
    7 amino acid substitution between VP1 452 and VP1…
    Promoter
    p41
    Available since
    Nov. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    8 Articles
    175004-plasmid-map-sequence-id-347131

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  48. pSNAP-tag (m) Vector

    Plasmid
    #101135
    Purpose
    Expression of protein of interest with SNAP-Tag in Mammalian cells
    Depositor
    New England Biolabs, Ana Egana
    Type
    Empty backbone
    Tags
    SNAP-tag (SNAP26m)
    Expression
    Mammalian
    Available since
    Sept. 27, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    11 Articles
    101135-plasmid-map-sequence-id-194101

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  49. pCAG-eCas9-GFP-U6-gRNA

    Plasmid
    #79145
    Purpose
    All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in human pluripotent stem cells
    Depositor
    Jizhong Zou
    Insert
    eSpCas9(1.1)
    Use
    CRISPR
    Tags
    2A-GFP
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    June 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    31 Articles
    79145-plasmid-map-sequence-id-182555

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  50. mCherry-LaminA-C-18

    Plasmid
    #55068
    Purpose
    Localization: Nuclear Envelope, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lamin A (LMNA Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    19 Articles
    55068-plasmid-map-sequence-id-200503

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Showing: 1001 - 1050 of 177145 results