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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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161,166 results

Showing: 1001 - 1050 of 161166 results

  1. pHR_SFFV-sig-mCherry-PDGFRTM-WPRE

    Plasmid
    #216668
    Purpose
    Expresses membrane-bound mCherry in mammalian cells
    Depositor
    Leonardo Morsut
    Insert
    mCherry
    Use
    Tags
    PDGFR Transmembrane Domain
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    216668-plasmid-map-sequence-id-432187

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  2. TFORF1500

    Plasmid
    #143960
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    RBL2 (RBL2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    143960-plasmid-map-sequence-id-404618

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  6. CASR-Tango

    Plasmid
    #66235
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    CASR (CASR Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66235-plasmid-map-sequence-id-378710

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  11. Epac-S-H188

    Plasmid
    #170349
    Purpose
    mT2Del_EPACdDEPCD_tdcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Insert
    mT2Del_EPACdDEPCD_tdcp173Ven(ST) (RAPGEF3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    170349-plasmid-map-sequence-id-344647

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  14. pLenti-CD74-ROS1 G2032R

    Plasmid
    #183814
    Purpose
    Express CD74-ROS1 fusion (C6;R34) harboring ROS1 G2032R mutation in mammalian cells
    Depositor
    Aaron Hata
    Article
    Lin et al Clin Cancer Res. 2021 May 15;27(10):2899
    Inserts
    ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
    CD74 molecule (CD74 Human)
    Use
    Retroviral
    Tags
    The fusion protein of CD74 exon 1-6 and ROS1 exon…
    Expression
    Mammalian
    Mutation
    G2032R
    Promoter
    CMV
    Available since
    April 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    183814-plasmid-map-sequence-id-360438

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  22. pET-22b DT 51E/148K

    Plasmid
    #11081
    Depositor
    John Collier
    Insert
    DT
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    51E/148K, catalytically inactive
    Promoter
    Available since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    11081-plasmid-map-sequence-id-306912

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  27. nCas9-PPE

    Plasmid
    #140445
    Purpose
    For plant prime editing in wheat plants or monocotyledons protoplasts
    Depositor
    Caixia Gao
    Article
    Lin et al Nat Biotechnol. 2020 May;38(5):582-585.
    Insert
    nCas9(H840A)-M-MLV
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    H840A for Cas9; D200N, T306K, W313F, T330P and L…
    Promoter
    maize Ubiquitin-1
    Available since
    May 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    140445-plasmid-map-sequence-id-296171

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  29. THINGL pcDNA3.1

    Plasmid
    #230046
    Purpose
    Expressing the human version of TINGL biosensor for glucose (named THINGL) in mammalian cells
    Depositor
    Bas Teusink
    Article
    Botman et al bioRxiv 2024.11.29.626064
    Insert
    THINGL
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 23, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    230046-plasmid-map-sequence-id-458381

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  33. pcDNA3.1 VDAC1-TC

    Plasmid
    #211733
    Purpose
    Expresses tetracysteine tagged VDAC1 in mammalian cells. TC-tag is attached to the C-terminus of VDAC1.
    Depositor
    Roland Malli
    Article
    Pilic et al bioRxiv 2023.09.08.556841
    Insert
    Voltage-dependent anion channel 1 (VDAC1 )
    Use
    Tags
    Tetracysteine
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    211733-plasmid-map-sequence-id-417721

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  40. pAAV-hSyn-HA-hM4D(Gi)-IRES-mCitrine

    Plasmid
    #50464
    Purpose
    Gi-coupled hM4D-IRES-mCitrine under the control of human synapsin promote
    Depositor
    Bryan Roth
    Insert
    hM4D(Gi)-IRES-mCitrine (CHRM4 Human)
    Use
    AAV
    Tags
    HA
    Expression
    Mutation
    Promoter
    human Synapsin 1
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50464-plasmid-map-sequence-id-181128

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  41. pAAV-double floxed-eNpHR-EYFP-WPRE-pA

    Plasmid
    #20949
    Purpose
    Cre-activated AAV expression of halorhodopsin 2.0 (eNpHR) fused to EYFP for optogenetic inhibition
    Depositor
    Karl Deisseroth
    Has Service
    AAV9
    Insert
    halorhodopsin
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    Promoter
    EF1alpha
    Available since
    May 13, 2009
    Availability
    Academic Institutions and Nonprofits only
    20949-plasmid-map-sequence-id-200280

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  42. pcDNA3 H2B-mIFP T2A Mpro (wt)

    Plasmid
    #163079
    Purpose
    Expresses wild type Mpro (active) and a near-infrared fluorescent protein mIFP fused to H2B
    Depositor
    Xiaokun Shu
    Insert
    Wild type Mpro of SARS-CoV-2 and H2B-mIFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    163079-plasmid-map-sequence-id-320826

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  44. GIPR-DuET

    Plasmid
    #213252
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GIPR (GIPR Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213252-plasmid-map-sequence-id-430802

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  45. AK3L1

    Plasmid
    #39135
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    AK3L1 (AK3 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  47. AK3L1

    Plasmid
    #25541
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    AK3L1 (AK4 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 25, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 1001 - 1050 of 161166 results