Skip to main content
Addgene
Showing: 2001 - 2050 of 4058 results

  3. pET28a-SnoopCatcher

    Plasmid
    #72322
    Purpose
    Bacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag.
    Depositor
    Mark Howarth
    Article
    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p
    Insert
    pET28a-SnoopCatcher
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    changed G842T and D848G of S. pneumoniae adhesin …
    Promoter
    T7
    Available since
    Feb. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    72322-plasmid-map-sequence-id-137253

    Enlarge

  10. pSBbi-Bla

    Plasmid
    #60526
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: blasticidin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a/RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60526-plasmid-map-sequence-id-97520

    Enlarge

  16. p11-LacY-wtx1

    Plasmid
    #69056
    Purpose
    The reporter plasmid p11-LacY-wtx1 encodes the toxin CcdB gene under the inducible BAD promoter and a single copy of the endonuclease cleavage site which is readily extendable to multiple copies.
    Depositor
    Huimin Zhao
    Article
    Chen et al Nucleic Acids Res. 2005 Oct 6;33(18):e15
    Inserts
    ccdB (ccdB )
    lacY (lacY )
    Use
    Tags
    A177C
    Expression
    Mutation
    Promoter
    pBAD and pLac
    Available since
    Sept. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    69056-plasmid-map-sequence-id-372941

    Enlarge

  19. pdCas9-DNMT3A-EGFP

    Plasmid
    #71666
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Human, Synthetic, S. pyogenes)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71666-plasmid-map-sequence-id-129527

    Enlarge

  22. RcCMV/Rb

    Plasmid
    #1763
    Depositor
    Bob Weinberg
    Insert
    human Rb (RB1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 18, 2006
    Availability
    Academic Institutions and Nonprofits only
    1763-plasmid-map-sequence-id-194429

    Enlarge

  27. pAAV-Ef1a-DIO EYFP

    Plasmid
    #27056
    Depositor
    Karl Deisseroth
    Has Service
    AAV Retrograde, AAV1, AAV2, AAV5, and AAV9
    Insert
    EYFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2011
    Availability
    Academic Institutions and Nonprofits only
    27056-plasmid-map-sequence-id-126562

    Enlarge

  36. pENTR11 CA NFAT1

    Plasmid
    #11792
    Depositor
    Anjana Rao
    Insert
    NFAT1 (Nfatc2 Mouse)
    Use
    Tags
    HA
    Expression
    Bacterial
    Mutation
    Constitutively active: multiple serine to alanine…
    Promoter
    Available since
    Aug. 7, 2006
    Availability
    Academic Institutions and Nonprofits only
    11792-plasmid-map-sequence-id-229776

    Enlarge

  49. Lenti-LucOS

    Plasmid
    #22777
    Depositor
    Tyler Jacks
    Insert
    OVA-SIY
    Use
    Cre/Lox and Lentiviral
    Tags
    luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 14, 2009
    Availability
    Academic Institutions and Nonprofits only
    22777-plasmid-map-sequence-id-419746

    Enlarge

Showing: 2001 - 2050 of 4058 results