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167,150 results

Showing: 3651 - 3700 of 167150 results

  2. MAT2B

    Plasmid
    #39080
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MAT2B (MAT2B Human)
    Tags
    Tev His Flag
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  4. pRVdG-4Halo3Y

    Plasmid
    #52496
    Purpose
    Expresses eNpHR 3.0-EYFP
    Depositor
    Ian Wickersham
    Insert
    eNpHR 3.0-EYFP
    Use
    Rabies virus
    Tags
    EYFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    52496-plasmid-map-sequence-id-81432

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  9. BLMA-c800

    Plasmid
    #53631
    Purpose
    bacterial expression plasmid
    Depositor
    Nicola Burgess-Brown
    Insert
    BLMA
    Tags
    His-Tev
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;…
    Promoter
    T7
    Available Since
    June 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  21. B-HiFi SpCas9

    Plasmid
    #207362
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-HiFi SpCas9
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    R691A, amino acids 1005-1013 replaced with two gl…
    Promoter
    CBh
    Available Since
    Oct. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    207362-plasmid-map-sequence-id-410135

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  22. TFORF2281

    Plasmid
    #142929
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MYBBP1A (MYBBP1A Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142929-plasmid-map-sequence-id-397291

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  24. TFORF3155

    Plasmid
    #144631
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    HLX (HLX Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144631-plasmid-map-sequence-id-348116

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  37. pCMV_NESPylRS(AF)_hU6tRNAPyl

    Plasmid
    #223508
    Purpose
    PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon
    Depositor
    Edward Lemke
    Article
    Nikic et al Angew Chem Int Ed Engl. 2016 Dec 23;55(5
    Inserts
    NES Pyrrolysyl-tRNA Synthetase (AF) (pylS Methanosarcina mazei)
    tRNA Pyl
    Expression
    Mammalian
    Mutation
    Y306A; Y384F
    Promoter
    CVM, SP6 and U6
    Available Since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    223508-plasmid-map-sequence-id-445174

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  39. pCRISPomyces-2BD

    Plasmid
    #234428
    Purpose
    Plasmid for Streptomyces containing gene of modified Cas9-BD protein and sequence of sgRNA cloning template
    Depositor
    Min-Kyu Oh
    Article
    Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:
    Insert
    Modified Cas9 with polyaspartate linking
    Expression
    Bacterial
    Mutation
    Linked polyaspartate using glycine-serine linker
    Available Since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234428-plasmid-map-sequence-id-468848

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  45. p189

    Plasmid
    #65207
    Purpose
    Mutant EGFP expression plasmid for the creation of a heteroduplex DNA repair assay plasmid with pGEM5Z(+)-EGFP (p111). *Please see notes in comments section.
    Depositor
    LuZhe Sun
    Article
    Zhou et al Anal Biochem. 2009 May 1;388(1):167-9. d
    Insert
    EGFP
    Expression
    Bacterial and Mammalian
    Mutation
    Premature stop codon added to pGEM5Z(+)-EGFP (TGG…
    Promoter
    CMV
    Available Since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    65207-plasmid-map-sequence-id-490957

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  46. pGEM5Z(+)-EGFP

    Plasmid
    #65206
    Purpose
    EGFP expression plasmid (also called p111) for the creation of a heteroduplex DNA repair assay plasmid with p189. *Please see notes in comments section.
    Depositor
    LuZhe Sun
    Article
    Zhou et al Anal Biochem. 2009 May 1;388(1):167-9. d
    Insert
    EGFP
    Expression
    Bacterial and Mammalian
    Mutation
    Restriction enzyme recognition sites added either…
    Promoter
    CMV
    Available Since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    65206-plasmid-map-sequence-id-490956

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  48. pSPIH6

    Plasmid
    #190676
    Purpose
    E. coli plasmid for single step cloning of SUMO-peptide-intein (SPI) fusion proteins (contains SUMO and His-tagged intein)
    Depositor
    John Vederas
    Article
    Lamer et al BMC Biotechnol. 2023 Apr 5;23(1):11. doi
    Inserts
    Small ubiquitin-like modifier
    Mxe GyrA intein with C-terminal chitin binding domain and His tag
    Tags
    Chitin binding domain, His tag, and N-terminal Hi…
    Expression
    Bacterial
    Mutation
    Added C-terminal his tag and None
    Promoter
    T7
    Available Since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    190676-plasmid-map-sequence-id-383624

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Showing: 3651 - 3700 of 167150 results