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Plasmid#189923PurposeAAV genome encoding SaKKHABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installationDepositorInsertSaABE8e V106W
UseAAVTagsExpressionMutationnSaCas9 KKH, TadA ABE8e V106WPromoterEFSAvailable sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S5E2-GFP-fGFP (AAV PHP.eB)
Viral Prep#135631-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV-S5E2-GFP-fGFP (#135631). In addition to the viral particles, you will also receive purified pAAV-S5E2-GFP-fGFP plasmid DNA. Bicistronic expression of GFP and membrane-targeted GFP under the control of the E2 regulatory element. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsEGFPAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-AAV1.X1
Plasmid#209780Purposenon-standard AAV2 rep-AAV1-X1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV1-X1 VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MATR3
Plasmid#32880DepositorInsertMATR3 Matrin3 (MATR3 Human)
UseTagsFLAGExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJan. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFH2.2_mCherry-MS2x12
Plasmid#205521PurposeExpress mCherry-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)DepositorInsertmCherry
UseTagsExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
N174-MCS (Puro)
Plasmid#81068PurposeLentiviral vector for constitutive gene expression.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterEF-1αAvailable sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
P4-TadCBEd
Plasmid#225143PurposePackaging plasmid for TadCBEd in v3b PE-eVLP architectureDepositorInsertP4-TadCBEd
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Calreticulin-N-16
Plasmid#54023PurposeLocalization: Endoplasmic Reticulum, Excitation: 487, Emission: 509DepositorInsertCalreticulin (CALR Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 2, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL97
Plasmid#78576PurposeOver-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains strong constitutive ermEp promoterDepositorTypeEmpty backboneUseStreptomyces expressionTagsExpressionBacterialMutationPromoterermEp* promoter from S. erythraeaAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRK5 alpha6
Plasmid#16036DepositorInsertalpha6 (ITGA6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP2-ER-5
Plasmid#55294PurposeLocalization: Endoplasmic Reticulum, Excitation: 399, Emission: 456DepositorInsertER (CALR Human)
UseTagsmTagBFP2ExpressionMammalianMutationER Signal PeptidePromoterCMVAvailable sinceOct. 7, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAW12.lentiguide.GFP
Plasmid#104374PurposeGFP vector for cloning in guidesDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FGFR3
Plasmid#116742PurposeLentiviral expression of FGFR3DepositorInsertFGFR3 (FGFR3 Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
nAChR beta2 WT
Plasmid#24272DepositorInsertnAChR beta2 WT (Chrnb2 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
nAChR alpha4 WT
Plasmid#24271DepositorInsertnAChR alpha4 WT (Chrna4 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTD-NTwinStrep_Sm
Plasmid#45937Purposeuse to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tagDepositorTypeEmpty backboneUseTags6xHis and Twin-StrepExpressionBacterialMutationPromoterTrc (lactose/IPTG inducible)Available sinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-SV40NLSf-mScarletP2A-hLuc
Plasmid#218793PurposeAAV reporter genome plasmidDepositorInsertNLSf-mScarletP2A-hLuc
UseAAVTagsExpressionMutationPromoterAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEBTet_NADP-Snifit-cyto
Plasmid#113917PurposePlasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cellsDepositorInsertNADP-Snifit
UseTagsExpressionMammalianMutationPromoterCMV TetO2Available sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
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AAV-hSyn1-GCaMP6s-P2A-nls-dTomato (AAV1)
Viral Prep#51084-AAV1PurposeReady-to-use AAV1 particles produced from AAV-hSyn1-GCaMP6s-P2A-nls-dTomato (#51084). In addition to the viral particles, you will also receive purified AAV-hSyn1-GCaMP6s-P2A-nls-dTomato plasmid DNA. GCaMP6s calcium sensor and bicistronic, physically separate nuclear localized dTomato expression under a human synapsin1 promoter. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsnuclear dTomato (physically separate, not a fusion protein)Available sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSIH1-puro-STAT3 shRNA
Plasmid#26596DepositorInsertSTAT3 shRNA (STAT3 Human)
UseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceNov. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMDIAI
Plasmid#51655PurposeE coli genetic modificationDepositorInsertapramycin antibiotic resistance gene
UseGenetic modificationTagsExpressionMutationPromoterunknownAvailable sinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBa.Halo-LDLR
Plasmid#162717PurposeMammalian expression of human LDLRDepositorInsertLDLR (LDLR Human)
UseTagsHaloExpressionMammalianMutationnonePromoterChicken Beta actinAvailable sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
PM-FRB-mRFP-T2A-FKBP-5-ptase
Plasmid#40896DepositorInsertPM-FRB-mRFP-T2A-FKBP-5-ptase
UseTagsExpressionMutationPromoterAvailable sinceJan. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGM1190
Plasmid#69994PurposeOriT containing mobilizable vector for thiostrepton induced gene expression in StreptomycesDepositorInsertsaac
tsr
rep
sso
TipA promoter
dso
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
piggyBAC-5xTAPE-1-BlastR
Plasmid#175808PurposeEncodes 5xTAPE-1 construct that can be integrated using piggyBAC systemDepositorInsert5xTAPE-1 construct
UseTagsExpressionMammalianMutationPromoterU6Available sinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACBSR-hyg
Plasmid#87830PurposeExpresses lambda-red system with hyg selection markerDepositorInserthyg
UseTagsNoneExpressionBacterialMutationNonePromoterblaAvailable sinceAug. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXL001-TQM IL13 CAR
Plasmid#154054PurposeLentiviral expression of TQM IL13 CAR for CAR-T therapyDepositorInsertTQM IL13 CAR
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
iF53 PB-Zim3-dCas9-mScarlet-Hygro
Plasmid#204726PurposeConstitutive CRISPRi by Zim3-dCas9InsertZim3-dCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-Mito4x-jRCaMP1b
Plasmid#127873PurposeRed-shifted fluorescent reporter for mitochondrial matrix Ca2+DepositorInsertRed-shifted mitochondrial matrix Ca2+ indicator
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-mRuby3-Gal8-P2A-Zeo
Plasmid#150815PurposeMammalian expression of mRuby3-Galectin8 (Gal8) fusion proteinDepositorInsertmRuby3-Gal8-P2A-Zeo (LGALS8 Human, Synthetic)
UseTagsGal8 is fused to the c-terminus of mRuby3ExpressionMammalianMutationPromoterCAGAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
iF66 PB-LoxStopLox-Zim3-dCas9-mScarlet-hygro
Plasmid#204731PurposeCre-inducible CRISPRi by Zim3-dCas9InsertZim3-dCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_RHEB_WT
Plasmid#81257PurposeGateway Donor vector containing RHEB , part of the Target Accelerator Plasmid Collection.DepositorInsertRHEB (RHEB Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15b PKA Cat
Plasmid#14921DepositorInsertPKA catalytic subunit alpha (Prkaca Mouse)
UseTags6xHis and Thrombin cleavage siteExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2007AvailabilityAcademic Institutions and Nonprofits only -
pHCMV-VSVgSS-15F11-TM
Plasmid#207317PurposeFunctional incorporation of 15F11 scFv (anti-HA) into envelope of virionsDepositorInsert15F11 ScFv
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-Ribo-jGCaMP8s (AAV1)
Viral Prep#167572-AAV1PurposeReady-to-use AAV1 particles produced from AAV-hSyn-Ribo-jGCaMP8s (#167572). In addition to the viral particles, you will also receive purified AAV-hSyn-Ribo-jGCaMP8s plasmid DNA. Synapsin-driven neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
MIGR-RORgt
Plasmid#24069DepositorInsertRorgt (Rorc Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-gateway
Plasmid#51070Purposegateway adapted mammalian expression vectorDepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterSV40Available sinceFeb. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
mApple-LAMP1-phLuorin-N-8
Plasmid#54918PurposeLocalization: Lysosome Membrane, Excitation: 568, Emission: 592DepositorInsertLAMP1 phLuorin
UseTagsmAppleExpressionMammalianMutationLysosomes-20 double labeled with phLuorinPromoterCMVAvailable sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131A
Plasmid#69273PurposeGolden Gate entry vector; 1st gRNA under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGWB451
Plasmid#74833PurposeGateway cloning compatible binary vector for C-terminal fusion with G3GFP (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
[pSK131] pCOLADuet p14|MP1
Plasmid#136145Purposebacterial expression of p14 and MP1DepositorInsertp14 and MP1
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiVIPe4_dTomato_nlsdTomato (AAV PHP.eB)
Viral Prep#213855-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV_BiVIPe4_dTomato_nlsdTomato (#213855). In addition to the viral particles, you will also receive purified pAAV_BiVIPe4_dTomato_nlsdTomato plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the VIP interneuron-targeting enhancer E4. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsdTomatoAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132A
Plasmid#69274PurposeGolden Gate entry vector; 2nd gRNA under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Anillin
Plasmid#68027PurposeFull length Anillin rendered RNAi-resistant) in mammalian expression vector pEGFP-C1DepositorInsertAnillin (ANLN Human)
UseTagseGFPExpressionMammalianMutationsilent mutations at 798 5'-TGCC TCTTTGAATAAA…PromoterCMVAvailable sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
multi-shRNA vector
Plasmid#12391DepositorInsertmSOD2 shRNA (Sod2 Mouse)
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pFUW-tetO-Pu.1
Plasmid#139839Purposedoxycycline-inducible expression of mouse Pu.1 in mammalian cellsDepositorInsertPu.1 (Spi1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE-mCMVAvailable sinceApril 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ144
Plasmid#69296PurposeGolden Gate recipient and Gateway entry vector; assembly of 4 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETDuet_huCAD_ICADL (TevS)
Plasmid#100098Purposedual expression of human CAD and ICAD. The two caspase cleavage sites in ICAD were mutated to TEVP cleavable sequencesDepositorUseTagsExpressionBacterialMutationtwo caspase cleavage sites (DETD-117 and DAVD-224…PromoterT7Available sinceJan. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.hSyn.Cre.WPRE.hGH (AAV2)
Viral Prep#105553-AAV2PurposeReady-to-use AAV2 particles produced from pENN.AAV.hSyn.Cre.WPRE.hGH (#105553). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.Cre.WPRE.hGH plasmid DNA. hSyn-driven Cre expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only