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Showing: 451 - 500 of 158784 results

  1. pcDNA3.1(+)/Luc2=tdT

    Plasmid
    #32904
    Purpose
    Expresses a fusion of firefly luciferase and tandem Tomato red fluorescent protein in mammalian cells.
    Depositor
    Christopher Contag
    Article
    Patel et al Cancer Res. 2010 Dec 15;70(24):10141-9.
    Inserts
    Firefly Luciferase
    tandem Tomato Red Fluorescent Protein
    Use
    Tags
    tdTomato red fluorescent protein
    Expression
    Mammalian
    Mutation
    Promoter
    CMV-IE and n/a
    Available since
    Feb. 1, 2012
    Availability
    Academic Institutions and Nonprofits only
    32904-plasmid-map-sequence-id-229770

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  4. TLCV2-DelGFP

    Plasmid
    #133302
    Purpose
    EGFP reporter was removed in the TLCV2 vector so it could be used for inducible gene CRISPR knockout in cell lines with an eGFP reporter.
    Depositor
    Jingshi Shen
    Article
    Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    133302-plasmid-map-sequence-id-271497

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  6. pcDNA-mC/EBPb

    Plasmid
    #49198
    Purpose
    expression of mouse C/EBPbeta in mammalian cells
    Depositor
    Jed Friedman
    Insert
    C/EBPbeta (Cebpb Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 14, 2013
    Availability
    Academic Institutions and Nonprofits only
    49198-plasmid-map-sequence-id-265902

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  9. pCMV3flag8HOIL-1L

    Plasmid
    #50016
    Purpose
    Mammalian expression of human HOIL-1L (RBCK1) with a c-terminal 3xFLAG tag, and hygromycin selection.
    Depositor
    Martin Dorf
    Article
    Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2
    Insert
    RanBP-type and C3HC4-type zinc finger containing 1 HOIL-1L (RBCK1 Human)
    Use
    Tags
    3 x FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 31, 2013
    Availability
    Academic Institutions and Nonprofits only
    50016-plasmid-map-sequence-id-169718

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  11. pLenti Lifeact-mTagBFP2 PuroR

    Plasmid
    #101893
    Purpose
    Lentiviral expression of mTagBFP2-tagged Lifeact with Puromycin selection in cells including neurons
    Depositor
    Ghassan Mouneimne
    Insert
    Lifeact
    Use
    Lentiviral
    Tags
    mTagBFP2
    Expression
    Mutation
    Promoter
    CMV immediate early
    Available since
    May 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    101893-plasmid-map-sequence-id-211159

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  12. pR_LG lenti-(empty)-del-CTS

    Plasmid
    #112895
    Purpose
    Non-integrating lentiviral co-packaging plasmid designed to reduce multiple integrations and recombination during transduction of nucleic acid libraries
    Depositor
    Paul Blainey
    Article
    Feldman et al bioRxiv 262121
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    none
    Available since
    Sept. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    112895-plasmid-map-sequence-id-217829

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  17. pCMV-T7-ABE8e-SpRY-HiFi-P2A-EGFP (LM458)

    Plasmid
    #197508
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpRY-HiFi(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e-nSpRY-HiFi-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpRY-HiFi(D10A/A61R/…
    Promoter
    CMV and T7
    Available since
    March 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    197508-plasmid-map-sequence-id-387292

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  20. mCherry-Integrin-Beta1-N-18

    Plasmid
    #55064
    Purpose
    Localization: Integrin/Focal Adhesions, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Integrin-Beta1 (ITGB1 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    55064-plasmid-map-sequence-id-313234

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  34. pCAGGs-BFP

    Plasmid
    #127348
    Purpose
    Expresses BFP constitutively via the CAGGS promoter in mammalian cells
    Depositor
    Phil Sharp
    Insert
    Blue Fluorescent Protein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAGGS Promoter
    Available since
    March 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    127348-plasmid-map-sequence-id-246576

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  36. pGAPZa-STE2-1D4

    Plasmid
    #133422
    Purpose
    Mutant form of yeast STE2 coding sequence in vector for protein expression in the methanogenic yeast Pichia pastoris, with GAP promoter.
    Depositor
    Gerhard Wagner
    Insert
    STE2 (STE2 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    C59S, C252A
    Promoter
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    133422-plasmid-map-sequence-id-267645

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  45. TFORF1864

    Plasmid
    #143550
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    POU2F2 (POU2F2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143550-plasmid-map-sequence-id-391396

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  47. pRSFDuet-sumo-NSP12 (SARS-CoV-2)

    Plasmid
    #159107
    Purpose
    The SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).
    Depositor
    Thomas Tuschl
    Article
    Chen et al bioRxiv. 2020 Jul 13. doi: 10.1101/2020.
    Insert
    NSP12
    Use
    Tags
    His-SUMO tag
    Expression
    Bacterial
    Mutation
    None
    Promoter
    T7
    Available since
    Sept. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    159107-plasmid-map-sequence-id-310025

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  49. TFORF2593

    Plasmid
    #142336
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    BHLHE40 (BHLHE40 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142336-plasmid-map-sequence-id-397187

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Showing: 451 - 500 of 158784 results