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8,883 results

Showing: 1 - 50 of 8883 results

  1. psPAX2

    Plasmid
    #12260
    Purpose
    2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259)
    Depositor
    Didier Trono
    Type
    Empty backbone
    Use
    Lentiviral; Packaging
    Expression
    Mammalian
    Available Since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12260-plasmid-map-sequence-id-467229

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  2. pMD2.G

    Plasmid
    #12259
    Purpose
    VSV-G envelope expressing plasmid
    Depositor
    Didier Trono
    Insert
    VSV G
    Use
    Lentiviral; Envelope
    Expression
    Mammalian
    Available Since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12259-plasmid-map-sequence-id-443804

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  6. pEVOL-pAzF

    Plasmid
    #31186
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAG
    Depositor
    Peter Schultz
    Article
    Chin et al J Am Chem Soc. 2002 Aug 7;124(31):9026-7
    Insert
    M.j. p-azidohenylalanine RS (2 copies +tRNA)
    Expression
    Bacterial
    Mutation
    Y32T, E107N, D158P, I159L, L162Q, D286R
    Available Since
    Dec. 9, 2011
    Availability
    Academic Institutions and Nonprofits only
    31186-plasmid-map-sequence-id-334781

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  9. pGL3 Basic Vector

    Plasmid
    #212936
    Purpose
    Firefly luciferase vector for investigating regions controlling transcription
    Depositor
    Debrya Groskreutz
    Type
    Empty backbone
    Use
    Luciferase
    Mutation
    WT
    Available Since
    Jan. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    212936-plasmid-map-sequence-id-419196

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  11. PB-TO-ASCL1-DLX2

    Plasmid
    #182307
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into GABAergic neuron via ASCL1 and DLX2 expression
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Article
    van Voorst et al bioRxiv [Preprint]. 2025 Jul 7:2025.06.3
    Inserts
    Ascl1 (Ascl1 Mouse)
    Dlx2 (Dlx2 Mouse)
    puroR-T2A-mycNLS-mTagBFP2
    Tags
    None and T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Promoter
    EF1a and pTRE3G-bidirectional
    Available Since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    182307-plasmid-map-sequence-id-359517

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  14. pLKO.1 puro

    Plasmid
    #8453
    Purpose
    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses puromycin for selection.
    Depositor
    Bob Weinberg
    Article
    Stewart et al RNA 2003 Apr;9(4):493-501.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Available Since
    July 18, 2005
    Availability
    Academic Institutions and Nonprofits only
    8453-plasmid-map-sequence-id-237918

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  16. pcDNA GNSTM-3-RVG-10-Lamp2b-HA

    Plasmid
    #71294
    Purpose
    Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, RVG peptide, 10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.
    Depositor
    Joshua Leonard
    Article
    Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72
    Insert
    Lamp2b (LAMP2 Human)
    Tags
    GNSTM glycosylation motif, HA, Lamp2 signal pepti…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    71294-plasmid-map-sequence-id-418480

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  23. lentiCRISPR v2

    Plasmid
    #52961
    Purpose
    Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Inserts
    Cas9
    Puromycin resistance
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    EFS-NS
    Available Since
    May 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    52961-plasmid-map-sequence-id-244694

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  29. CROPseq-Guide-Puro

    Plasmid
    #86708
    Purpose
    Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.
    Depositor
    Christoph Bock
    Article
    Datlinger et al Nat Methods. 2017 Jan 18. doi: 10.1038/n
    Insert
    EF1a-Puro-WPRE-hU6-gRNA
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    hU6
    Available Since
    Feb. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    86708-plasmid-map-sequence-id-317051

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Showing: 1 - 50 of 8883 results