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169,449 results

Showing: 1151 - 1200 of 169449 results

  1. pET-MCN-10His-mStayGold (E138D)

    Plasmid
    #211361
    Purpose
    Bacterial expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.
    Depositor
    Mohan Balasubramanian
    Article
    Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103
    Insert
    mStayGold
    Tags
    10xHis-tag
    Expression
    Bacterial
    Mutation
    E138D
    Promoter
    T7
    Available Since
    Dec. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    211361-plasmid-map-sequence-id-417678

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  5. ER-GA

    Plasmid
    #209871
    Purpose
    Dimerization dependent fluorescent protein GA anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1
    Depositor
    Sarah Cohen
    Article
    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25
    Insert
    ddGFP A
    Tags
    Targeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    209871-plasmid-map-sequence-id-415464

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  7. ADORA2A-Tango

    Plasmid
    #66210
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    ADORA2A (ADORA2A Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. T7-VEE-OKS-iG

    Plasmid
    #58974
    Purpose
    RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 and a second IRES and PuroR ORF
    Depositor
    Steven Dowdy
    Article
    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.
    Inserts
    Oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    GLIS (GLIS1 Human)
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Expression
    Mammalian
    Available Since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    58974-plasmid-map-sequence-id-240741

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  11. pTrex-n-eGFP

    Plasmid
    #62544
    Purpose
    Expression of eGFP in T. cruzi. The NheI-linearized form of this plasmid could be efficiently integrated in to T. cruzi genome
    Depositor
    Rick Tarleton
    Article
    Peng et al MBio. 2014 Dec 30;6(1). pii: e02097-14.
    Insert
    eGFP
    Use
    (1)trypanosoma cruzi expression of egfp (2) genom…
    Tags
    None
    Mutation
    None
    Promoter
    Ribo-HX1
    Available Since
    April 16, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    62544-plasmid-map-sequence-id-109759

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  13. ER-RA

    Plasmid
    #209872
    Purpose
    Dimerization dependent fluorescent protein RA anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1
    Depositor
    Sarah Cohen
    Article
    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25
    Insert
    ddRFP A
    Tags
    Targeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    209872-plasmid-map-sequence-id-415475

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  14. TFORF2165

    Plasmid
    #141953
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    GRHL3 (GRHL3 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    141953-plasmid-map-sequence-id-391431

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  20. pDest-Sumo

    Plasmid
    #106980
    Purpose
    LR reaction to create expression plasmid. For prokaryotic expression of target protein as N-terminal fusion form with Sumo tag
    Depositor
    Han Choe
    Article
    Nguyen et al Sci Rep. 2017 Nov 23;7(1):16139. doi: 10
    Type
    Empty backbone
    Tags
    His8-Sumo
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    106980-plasmid-map-sequence-id-204719

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  26. pSupAR-MbPylRS(DiZPK)

    Plasmid
    #91705
    Purpose
    express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in E. coli to incorporate photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into an in-frame amber codon site on a protein of interest
    Depositor
    P. Chen
    Article
    Zhang et al Nat Chem Biol. 2011 Sep 4;7(10):671-7. d
    Inserts
    pylS
    pylT
    Expression
    Bacterial
    Mutation
    L274A, C313S
    Available Since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    91705-plasmid-map-sequence-id-183278

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  28. pDisplay-mgSrtA

    Plasmid
    #125792
    Purpose
    Transfected into cell of interest for membrane expression. The encoded mgSrtA could enable labeling of the interacting cells which is useful for recording cell-cell interactions.
    Depositor
    P. Chen
    Article
    Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18
    Insert
    mgSrtA
    Expression
    Mammalian
    Available Since
    Oct. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    125792-plasmid-map-sequence-id-258287

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  34. EF1α-eGFP-Z1

    Plasmid
    #135612
    Purpose
    Enhanced green fluorescent protein expression for mammalian cells under EF1a-HTLV promoter in minimal backbone plasmid.
    Depositor
    Jordan Green
    Insert
    eGFP
    Expression
    Mammalian
    Promoter
    EF1a-HTLV
    Available Since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135612-plasmid-map-sequence-id-269403

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  35. pCALNL-GFP

    Plasmid
    #13770
    Purpose
    Cre recombinase-dependent expression of GFP, i.e. GFP is only expressed in the presence of Cre due to a transcriptional stop in between the loxP sites.
    Depositor
    Connie Cepko
    Article
    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10
    Insert
    EGFP
    Use
    Cre/Lox
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    April 20, 2007
    Availability
    Academic Institutions and Nonprofits only
    13770-plasmid-map-sequence-id-7759

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  36. gGBEv6.3-SpCas9

    Plasmid
    #202629
    Purpose
    vector for encoding an engineered glycosylase-based guanine base editor (gGBEv6.3) with SpCas9 nickase driven by EF1a promoter, guide RNA compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Natl Sci Rev. 2023 May 16;10(8):nwad143.
    Insert
    nSpCas9(D10A)-MPGv6.3
    Expression
    Mammalian
    Mutation
    MPGv6.3 = G163R, N169G, D175R, C178N, S198A, K202…
    Promoter
    hU6, EF1a, CBh
    Available Since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    202629-plasmid-map-sequence-id-398425

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  38. pT7-V5-SBP-C1-HsPTB1

    Plasmid
    #64925
    Purpose
    Expresses V5- and SBP-tagged PTB1 in mammalian cells
    Depositor
    Elisa Izaurralde
    Insert
    PTB1 (PTBP1 Human)
    Tags
    V5 and SBP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    64925-plasmid-map-sequence-id-109671

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  42. ADORA2B-Tango

    Plasmid
    #66211
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    ADORA2B (ADORA2B Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66211-plasmid-map-sequence-id-361672

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  45. GPR4-Tango

    Plasmid
    #66358
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    GPR4 (GPR4 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  46. AICSDP-42:AAVS1-mTagRFPT-CAAX

    Plasmid
    #107580
    Purpose
    Homology arms, promoter and linker-mTagRFPT-CAAX sequence for internal insertion of CAGGS-driven mTagRFPT-CAAX at the AAVS1 safe harbor location in human cells
    Depositor
    Allen Institute for Cell Science
    Insert
    AAVS1 Homology Arms with CAGGS-driven mTagRFPT-CAAX (AAVS1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    May 9, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    107580-plasmid-map-sequence-id-208159

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  50. pAAV2/1

    Plasmid
    #112862
    Purpose
    AAV packaging plasmid expressing Rep/Cap genes
    Depositor
    James M. Wilson
    Insert
    Rep2/Cap1
    Use
    AAV
    Promoter
    Truncated P5
    Available Since
    Aug. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    112862-plasmid-map-sequence-id-421935

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Showing: 1151 - 1200 of 169449 results