Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

162,717 results

Showing: 1151 - 1200 of 162717 results

  3. pHS2056

    Plasmid
    #239837
    Purpose
    OMEGAOff + omegaRNA scaffold AAV expression
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038
    Insert
    DNMT3A-DNMT3L-NovaIscB-KRAB::NovaIscB omegaRNA scaffold
    Use
    AAV
    Tags
    HA and SV40 NLS
    Expression
    Mutation
    Insertion of REC domain from NbaCas9-1 + E137K/E4…
    Promoter
    EFS; human U6
    Available since
    July 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    239837-plasmid-map-sequence-id-479103

    Enlarge

  5. pET23b-15F11-HA-mEGFP-6xHis

    Plasmid
    #129593
    Purpose
    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.
    Depositor
    Tim Stasevich
    Article
    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:
    Insert
    Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
    Use
    Tags
    6xHis-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    129593-plasmid-map-sequence-id-254271

    Enlarge

  6. pCS2+mNeonGreen-C Cloning Vector

    Plasmid
    #128144
    Purpose
    To express a protein of interest fused to the C-terminus of mNeonGreen
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Use
    Tags
    mNeonGreen fusion
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    128144-plasmid-map-sequence-id-248605

    Enlarge

  7. LV-AR

    Plasmid
    #213819
    Purpose
    Lenti-viral expression of human androgen receptor wild-type (WT).
    Depositor
    Steven Balk
    Article
    Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:
    Insert
    AR (human androgen receptor) (AR Human)
    Use
    Lentiviral
    Tags
    Flag (N terminal on backbone)
    Expression
    Mammalian
    Mutation
    No, it is AR wild-type.
    Promoter
    EF-1a
    Available since
    Feb. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    213819-plasmid-map-sequence-id-422263

    Enlarge

  12. AAVS1-tdTomato

    Plasmid
    #159275
    Purpose
    Human AAVS1 targeting vector for knockin of a CAGGS promoter-driven tdTomato cassette (cloned between AgeI and PacI). Targeted cells will be puromycin resistant.
    Depositor
    Rudolf Jaenisch
    Article
    Ma et al Proc Natl Acad Sci U S A. 2018 Apr 10;11
    Insert
    tdTomato
    Use
    AAV; S1 knockin donor vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAGGS
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    159275-plasmid-map-sequence-id-312908

    Enlarge

  29. pUBC-SV40NLS-Pfv-Sapphire-PURO

    Plasmid
    #203654
    Purpose
    Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a puromycin selection marker.
    Depositor
    Liam Holt
    Article
    Shu T et et al bioRxiv 2022
    Insert
    PfV
    Use
    Lentiviral
    Tags
    Sapphire
    Expression
    Mutation
    Promoter
    Available since
    July 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    203654-plasmid-map-sequence-id-402486

    Enlarge

  30. EF1a mCherry

    Plasmid
    #129431
    Purpose
    expression of mCherry from EF1a promoter
    Depositor
    Oskar Laur
    Insert
    mCherry
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    129431-plasmid-map-sequence-id-256111

    Enlarge

  32. pcDNA5/FRT/TO-GAlphaQ-RLuc8

    Plasmid
    #140982
    Purpose
    Encodes a G alpha subunit (GNAQ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphaQ-RLuc8 (GNAQ Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140982-plasmid-map-sequence-id-300744

    Enlarge

  36. pDG459

    Plasmid
    #100901
    Purpose
    SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-PuroR
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100901-plasmid-map-sequence-id-197197

    Enlarge

  44. pCRISPRi_Mxi1_yl

    Plasmid
    #91248
    Purpose
    CRISPR-dCas9-Mxi1 vector for Yarrowia lipolytica, expressing dCas9-Mxi1 and AvrII site for sgRNA insertion
    Depositor
    Ian Wheeldon
    Article
    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.
    Inserts
    Codon optimized dCas9-Mxi1
    sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available since
    Aug. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    91248-plasmid-map-sequence-id-192174

    Enlarge

  46. pBbB8k-csg-NbGFP

    Plasmid
    #166858
    Purpose
    Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a GFP-specific nanobody via a flexible linker. Derived from https://www.addgene.org/35363/.
    Depositor
    Neel Joshi
    Article
    Birnbaum et al Adv Sci (Weinh). 2021 May 3;8(11):200469
    Insert
    csgBACEFG, with GFP-specific nanobody fused to CsgA via flexible linker
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    166858-plasmid-map-sequence-id-329027

    Enlarge

Showing: 1151 - 1200 of 162717 results