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177,145 results

Showing: 1151 - 1200 of 177145 results

  7. AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep

    Plasmid
    #92099
    Purpose
    Doyon lab Tandem-Affinity Purification Following Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus. Transgene expression is controlled by an Auto-Regulated Tet-On 3G System.
    Depositor
    Yannick Doyon
    Publication
    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15
    Type
    Empty backbone
    Use
    CRISPR and TALEN; Zfn
    Expression
    Mammalian
    Promoter
    Tet-On 3G Bidirectional
    Available since
    June 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    8 Articles
    92099-plasmid-map-sequence-id-180428

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  9. pDest-527

    Plasmid
    #11518
    Purpose
    Gateway destination vector for bacterial expression; His tag
    Depositor
    Dominic Esposito
    Type
    Empty backbone
    Use
    Gateway Cloning
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    May 24, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    26 Articles
    11518-plasmid-map-sequence-id-181065

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  11. SARS-CoV-2 S HexaPro

    Plasmid
    #154754
    Purpose
    Mammalian expression vector for expression of the SARS-CoV-2 spike HexaPro variant
    Depositor
    Jason McLellan
    Publication
    Hsieh et al Science. 2020 Sep 18;369(6510):1501-1505
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Spike (HexaPro variant) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …
    Expression
    Mammalian
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Available since
    July 1, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    56 Articles
    154754-plasmid-map-sequence-id-304640

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  15. pCMV CEPIA3mt

    Plasmid
    #58219
    Purpose
    Green fluorescent indicator for calcium imaging in the mitochondria (moderately low affinity variant)
    Depositor
    Masamitsu Iino
    Publication
    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CEPIA3mt
    Tags
    myc
    Expression
    Mammalian
    Mutation
    GCaMP2 N263Y E282V E334D M339L
    Promoter
    CMV
    Available since
    July 18, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    23 Articles
    58219-plasmid-map-sequence-id-212578

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  17. CMV-G-GECO1.2

    Plasmid
    #32446
    Purpose
    Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.2)
    Depositor
    Robert Campbell
    Publication
    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    G-GECO1.2
    Expression
    Mammalian
    Mutation
    GCaMP3 K69E/N77Y/D86G/K119I/L173Q/D260G/S404G/E43…
    Promoter
    CMV
    Available since
    Sept. 29, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    32446-plasmid-map-sequence-id-122736

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  20. pSBbi-Pur

    Plasmid
    #60523
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: puromycin resistance gene
    Depositor
    Eric Kowarz
    Publication
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    32 Articles
    60523-plasmid-map-sequence-id-305758

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  34. pCAS

    Plasmid
    #60847
    Purpose
    Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast
    Depositor
    Jamie Cate
    Publication
    Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife
    Inserts
    S. pyogenese Cas9
    RNA pol III promoter (tRNA-Tyr)
    hepatitis delta virus ribozyme, genomic
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    NLS/His8 and TTT 3' extension prior to sgRNA
    Expression
    Bacterial and Yeast
    Mutation
    L4 is UUCG tetraloop and guide targets LYP1 (CATA…
    Available since
    Jan. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    31 Articles
    60847-plasmid-map-sequence-id-197511

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  35. pBS-Hsp70-Cas9

    Plasmid
    #46294
    Purpose
    A codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.
    Depositor
    Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger
    Publication
    Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1
    Insert
    codon optimized Cas9
    Use
    CRISPR
    Tags
    3x Flag
    Expression
    Insect
    Mutation
    Silent change from C to A at bp 834 of insert
    Promoter
    Hsp70
    Available since
    July 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    36 Articles
    46294-plasmid-map-sequence-id-332831

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Showing: 1151 - 1200 of 177145 results