162,586 results
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Plasmid#117504PurposeYAO:Cas9:E9 Golden Gate Level 1 Position 2 reverseDepositorInsertBpiI:tagt:YAO:Cas9-3(intron):E9:ttgc:BpiI
UseCRISPRTagsExpressionPlantMutationPromoterAtYAOAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSPORT1[attB/Ins1/NbT promoter/glGFP/bglobin 3'-UTR/Ins2]
Plasmid#74102Purposeplasmid driven under neuronal beta-tubulin promoter, bearing attB and two insulator sequences (opposite directions pointing inward towards the reporter gene)DepositorInsertsXenopus laevis beta-tubulin gene promoter region and 5'UTR
Green Lantern Green Fluorescent Protein
Rabbit beta 1-globin gene 3'UTR
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-Cezanne2 (OTU+UBA, aa 1-462)
Plasmid#61582PurposeExpresses human Cezanne2 (OTU+UBA domains) in E. coli.DepositorInsertCezanne2 (OTUD7A Human)
UseTagsHis6-GST-3CExpressionBacterialMutationIsoform 2. Deleted aa 463-933.PromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMZ-180
Plasmid#195428PurposeTransient, constitutive expression of synZiFTR: ZF5-p65DepositorInsertpUb - 3XFLAG - NLS - ZF5-7 (ZF5) - p65 - RbGpA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Dub3 mouse
Plasmid#72682Purposemammalian expression of mouse Dub3DepositorInsertDub3 (Usp17le Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMN-013
Plasmid#195417PurposeAAVS1 Integratable mCherry Reporter for synZiFTR ZF5DepositorInsert4X ZF5 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-ZmBbm-9
Plasmid#197748PurposePhytobrick (MoClo) Level 0 PartDepositorInsertZmBbm
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
LBJJ383
Plasmid#117517PurposePCR template for sgRNA classic with polyT as terminatorDepositorInsertBpiI:ACTA:pU6-26:sgRNA:polyT:TTAC:BpiI
UseCRISPRTagsExpressionPlantMutationPromoterAtU6-26Available sinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV[Exp]-Puro-CMV>{5-HT6-R_GECO1.1}
Plasmid#232879PurposeExpresses the 5-HT6R coupled to the RGECO1 calcium sensorDepositorInsert5-Hydroxytryptamine Receptor 6 (HTR6 Human)
UseLentiviralTagsRGECO1.1ExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
PB-3-FT-ENGRAM
Plasmid#179158PurposepiggyBac plasmid for 3'-FT ENGRAM recorderDepositorInsertCsy4
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterminPAvailable sinceDec. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
etnppl-pGEMtEasy
Plasmid#192263PurposeMouse Etnppl sequence is inserted in pGEM-T Easy vector.DepositorInsertethanolamine phosphate phospholyase (Etnppl Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-hTERT-IRES-hygro
Plasmid#85140PurposeLentiviral expression of hTERT, used to create immortalized cell linesDepositorInserthTERT (TERT Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2GFP
Plasmid#82416Purpose3rd generation lentiviral vector expressing GFP alongside Cas9 and an sgRNA cloning siteDepositorInsertGFP
UseCRISPR and LentiviralTagsExpressionMutationPromoterEFS (P2A)Available sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro (PX459) V2.0
Plasmid#62988PurposeCas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)DepositorHas ServiceCloning Grade DNAInserthSpCas9-2A-Puro V2.0
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationPromoterCbhAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Puro
Plasmid#52963PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.DepositorHas ServiceCloning Grade DNAInsertsS. pyogenes sgRNA cassette
Puromycin resistance
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEf1-a and hU6Available sinceMay 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pU6-pegRNA-GG-acceptor
Plasmid#132777PurposePrime editing in mammalian cellsDepositorInsertExchangeable cassette
UseTagsExpressionMammalianMutationSee manuscriptPromoterU6Available sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV2/9n
Plasmid#112865PurposeAAV packaging plasmid expressing Rep/Cap genesDepositorInsertRep2/Cap9
UseAAVTagsExpressionMutationPromoterTruncated P5Available sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV2/5
Plasmid#104964PurposeAAV packaging plasmid expressing Rep/Cap genes for production of serotype 5DepositorInsertRep2/Cap5
UseAAVTagsExpressionMammalianMutationPromoterp5Available sinceOct. 2, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCL-Eco
Plasmid#12371DepositorInsertgag/pol/env
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE7
Plasmid#214812PurposeMammalian expression of SpCas9 PE7 prime editorDepositorInsertPE7
UseTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationPromoterCMVAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
FHRE-Luc
Plasmid#1789PurposeReporter plasmid for FOXO3a. Forkhead responsive element with luciferase readout.DepositorInsertFHRE (FOXO3 Human)
UseLuciferaseTagsExpressionMammalianMutation3 copies of FREPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ATLASsnCre
Plasmid#232351PurposeAnterograde transsynaptic tracer protein to express in presynaptic glutamatergic neurons, Cre payload. Must use with AAV8-DIO-Reporter viruses infected in postsynaptic cells.DepositorInsertATLASsnCre
UseAAVTagsALFA-TagExpressionMutationPromoterhSynAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-ATLASsnFlp
Plasmid#232352PurposeAnterograde transsynaptic tracer protein to express in presynaptic glutamatergic neurons that express Cre, Flp payload. Must use with AAV8-fDIO-Reporter viruses infected in postsynaptic cells.DepositorInsertATLASsnFlp
UseAAVTagsALFA-TagExpressionMutationPromoterhSynAvailable sinceMay 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-BACE-HA
Plasmid#232353PurposeBeta-Secretase to be expressed (1:1) with either ATLAS protein.DepositorInsertBACE (BACE1 Human)
UseAAVTagsHAExpressionMutationPromoterhSynAvailable sinceMay 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pU6-tevopreq1-GG-acceptor
Plasmid#174038PurposePrime editing in mammalian cellsDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterhU6Available sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pInducer20
Plasmid#44012PurposeTet-inducible lentiviral vector for ORF expressionDepositorTypeEmpty backboneUseLentiviralTagsHAExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV GFP Puro (658-5)
Plasmid#17448Purpose3rd gen lentiviral eGFP expression vector, CMV promoter, PuroDepositorHas ServiceCloning Grade DNAInsertGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-GFP
Plasmid#37825DepositorHas ServiceAAV CAP-B10, AAV CAP-B22, AAV MaCPNS1, AAV MaCPNS2, AAV PHP.eB, AAV Retrograde, AAV Retrograde trial size, AAV1, AAV1 trial size, AAV11, AAV11 trial size, AAV2, AAV2 trial size, AAV5, AAV5 trial size, AAV6, AAV6 trial size, AAV8, AAV8 trial size, AAV9, AAV9 trial size, and AAV9-X1.1InsertGFP
UseAAV; Adeno-associated virusTagsExpressionMammalianMutationN/APromoterCAGAvailable sinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 puro
Plasmid#8453Purpose3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses puromycin for selection.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2005AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-jGCaMP8s-WPRE
Plasmid#162377PurposeAAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expressionDepositorHas ServiceAAV Retrograde, AAV1, and AAV9InsertjGCaMP8s
UseAAV and Cre/LoxTags6xHisExpressionMammalianMutationS26M F286Y Q315HPromoterSynapsinAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3 Basic Vector
Plasmid#212936PurposeFirefly luciferase vector for investigating regions controlling transcriptionDepositorTypeEmpty backboneUseLuciferaseTagsExpressionMutationWTPromoterAvailable sinceJan. 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAG-ERT2CreERT2
Plasmid#13777PurposeMammalian expression of conditionally active form of Cre recombinase (activated in response to tamoxifen)DepositorInsertERT2-Cre-ERT2 fusion protein
UseCre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCMV_ENVLPE+
Plasmid#232427PurposeExpression plasmid to produce ENVLPE+ particlesDepositorInsertENVLPE+
UseTagsExpressionMammalianMutationPromoter2xCMVAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLX303-ZIM3-KRAB-dCas9
Plasmid#154472PurposeExpresses ZIM3 KRAB domain fused to the N terminus of dCas9DepositorInsertZIM3 (ZIM3 Synthetic)
UseLentiviralTagsHAExpressionMammalianMutationIncludes ZIM3 aa 1-100PromoterAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-NT*-HRV3CP
Plasmid#162795PurposeExpressess an optimized construct of human rhinovirus 14 3C protease (NT*-HRV3CP)DepositorInsertHuman rhinovirus 14 3C protease
UseTagsNT* solubility tagExpressionBacterialMutationPromoterT7Available sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
TLCV2
Plasmid#87360PurposeLentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.DepositorHas ServiceCloning Grade DNAInsertCas9-2A-eGFP
UseCRISPR and Lentiviral; Doxycycline inducible; egf…TagsCas9-T2A-eGFPExpressionMammalianMutationPromoterTight TRE promoterAvailable sinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
Dual-sgRNA_hU6-mU6
Plasmid#154194PurposeLentiviral expression plasmid for two sgRNAs from a hU6 and a mU6 promoter. The cloning site design allows a one-step cloning strategy of both sgRNAs. Expresses a Thy1.1 marker.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tet-pLKO-puro
Plasmid#21915Purpose3rd generation lentiviral plasmid for inducible expression of shRNA; puromycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER21 (ORF-EG)
Plasmid#46948PurposeInducible lentiviral gene expression vector. Chloramphenicol cm + ccdB cassette may be removed to clone in gene of interestDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
rtTA-N144
Plasmid#66810Purpose2nd generation lentiviral transfer plasmid. Expresses the reverse tetracycline-controlled transactivator (rtTA) with hygromycin resistanceDepositorInsertReverse tetracycline-controlled transactivator
UseLentiviralTagsExpressionMammalianMutationPromoterHuman EF1aAvailable sinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEcgRNA
Plasmid#166581PurposeCRISPR-Cas9-assisted genome editing in Escherichia coliDepositorInsertccdB
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRDA_550
Plasmid#203398PurposeAll-in-one lentiviral expression of gRNA and EnAsCas12aDepositorInsertEnhanced Acidaminococcus Cas12a
UseCRISPR and LentiviralTagsExpressionMutationPromoterEF1aAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9
Plasmid#47106PurposeExpresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cellsDepositorInsertdCas9
UseCRISPRTagsFlag and SV40 NLSExpressionMammalianMutationD10A, H840A (catalytically inactive)PromoterCMVAvailable sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
PB533-42B3mutAC2-sfGFP
Plasmid#186777PurposeRNAP2 Ser2ph-mintbody [42B3(R78K/A80T/M95V)-scFv] expression vector for mammalian cellsDepositorInsert42B3mutAC2-scFv
UseTagssfGFPExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET His6 MBP TEV LIC cloning vector (1M)
Plasmid#29656DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTags6xHis, MBP, and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacO (lactose/IPTG inducible)Available sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCas9
Plasmid#42876PurposeBacterial expression of Cas9 nuclease, tracrRNA and crRNA guideDepositorInserttracr/Cas9
UseCRISPR; E.coliTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEcCas
Plasmid#73227PurposeConstitutive expression of cas9 and inducible expression of lambda RED and sgRNADepositorInsertsCas9
sgRNA
UseCRISPR; Red recombinaseTagsExpressionBacterialMutationPromoternative promoterAvailable sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Cre
Plasmid#123133PurposeMammalian expression vector for wild-type Cre.DepositorInsertCre recombinase
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET His6 TEV LIC cloning vector (2B-T)
Plasmid#29666DepositorTypeEmpty backboneUseTagsHis6-TEVExpressionBacterialMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPL7552_ColE1_PiggyBac_2xcHS4::mCherry-IRES-PuroR-P2A-mTagBFP2-NLS::2xcHS4
Plasmid#217971PurposeProvides expression from a CMV promoter surrounded by insulators. Integrates via piggyBac transposase. Confers puromycin resistance. Expresses nuclear localized BFP. High copy plasmid.DepositorInsert2xcHS4::mCherry-IRES-PuroR-mTagBFP2-NLS::2xcHS4
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only