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19,771 results

Showing: 351 - 400 of 19771 results

  6. CROP-seq-opti

    Plasmid
    #106280
    Purpose
    A version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.
    Depositor
    Jay Shendure
    Article
    Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460
    Insert
    EF1a-Puro-WPRE-hU6-gRNA
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    106280-plasmid-map-sequence-id-354474

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  10. pCMV-PEmax-P2A-hMLH1dn

    Plasmid
    #174828
    Purpose
    Mammalian expression of SpCas9 PEmax prime editor with P2A-human MLH1dn (codon optimized)
    Depositor
    David Liu
    Article
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Insert
    PEmax-P2A-hMLH1dn
    Tags
    SV40 bpNLS and c-Myc NLS
    Expression
    Mammalian
    Mutation
    Detailed in manuscript; deletion of residues 754-…
    Promoter
    CMV
    Available Since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    174828-plasmid-map-sequence-id-357934

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  11. VR124 pETv2 10His-pG-Tn5 (E54K, L372P)

    Plasmid
    #198468
    Purpose
    E. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in T7 Express lysY/Iq protein expression strain.
    Depositor
    Viviana Risca
    Article
    Soroczynski et al bioRxiv 2024.07.11.602973
    Insert
    10His-pG-Tn5 (E54K, L372P)
    Tags
    10His-pG
    Expression
    Bacterial
    Mutation
    E54K, L372P
    Promoter
    Consensus φ10 T7 Promoter
    Available Since
    April 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    198468-plasmid-map-sequence-id-390256

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  16. pAAV2/2

    Plasmid
    #232199
    Purpose
    AAV packaging plasmid expressing AAV2 Rep and capsid proteins
    Depositor
    Melina Fan, Addgene Research Program
    Insert
    Rep2/Cap2
    Use
    AAV
    Expression
    Mammalian
    Available Since
    Sept. 18, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    232199-plasmid-map-sequence-id-457007

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  21. pcDNA3-EGFP

    Plasmid
    #13031
    Purpose
    Mammalian expression vector for cloning your gene fused to EGFP
    Depositor
    Doug Golenbock
    Insert
    Enhanced Green Fluorescent Protein
    Expression
    Mammalian
    Available Since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13031-plasmid-map-sequence-id-305137

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  30. pSBbi-Bla

    Plasmid
    #60526
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: blasticidin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available Since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60526-plasmid-map-sequence-id-97520

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  45. MSCV-IRES-GFP

    Plasmid
    #20672
    Purpose
    Depositor
    Tannishtha Reya
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    IRES EGFP
    Expression
    Mammalian
    Available Since
    Aug. 28, 2009
    Availability
    Academic Institutions and Nonprofits only
    20672-plasmid-map-sequence-id-203241

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  48. MBP-Benzonase

    Plasmid
    #218192
    Purpose
    Bacterial expression of a periplasmically expressed MBP-tagged Serratia Marcescens NucA (Benzonase); also known as c001
    Depositor
    Cheryl Arrowsmith
    Article
    Rieubon et al protocols.io 2025
    Insert
    nucA
    Tags
    MBP
    Expression
    Bacterial
    Available Since
    April 24, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    218192-plasmid-map-sequence-id-431003

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  49. pcDNA3.1-(-)-Pur

    Plasmid
    #200458
    Purpose
    Eukaryotic expression vector
    Depositor
    Bertrand Collet
    Type
    Empty backbone
    Expression
    Mammalian
    Available Since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    200458-plasmid-map-sequence-id-394118

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Showing: 351 - 400 of 19771 results