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Showing: 351 - 400 of 19133 results

  9. pRL-SV40P

    Plasmid
    #27163
    Purpose
    Renilla SV40 promoter (no enhancer) reporter gene for normalization of luciferase experiments
    Depositor
    Ron Prywes
    Article
    Chen et al Mol Cell Biol. 1999 Jul . 19(7):4695-702
    Insert
    SV40 promoter
    Use
    Luciferase
    Tags
    Renilla Luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 25, 2011
    Availability
    Academic Institutions and Nonprofits only
    27163-plasmid-map-sequence-id-419582

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  10. pAAV-EF1a-DIO-hM4D(Gi)-mCherry

    Plasmid
    #50461
    Purpose
    Double floxed Gi-coupled hM4D DREADD fused with mCherry under the control of EF1a promoter
    Depositor
    Bryan Roth
    Insert
    hM4D(Gi)-mCherry (CHRM4 Human)
    Use
    AAV
    Tags
    HA and mCherry
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    EF1a
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50461-plasmid-map-sequence-id-182308

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  18. AAVS1-DRGFP

    Plasmid
    #113193
    Purpose
    DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.
    Depositor
    Adam Karpf
    Insert
    DR-GFP
    Use
    Donor construct with t2a-hygromycin for gene trap…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    113193-plasmid-map-sequence-id-216317

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  19. pAGT8163 (AATG_ttLbCas12a-i_TTCG)

    Plasmid
    #192453
    Purpose
    intronized ttLbCas12a for in planta expression
    Depositor
    Tom Schreiber
    Article
    Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4
    Insert
    temperature-tolerant LbCas12a with introns (BsaI-flanked Golden Gate module, MoClo L0)
    Use
    CRISPR and Synthetic Biology; Moclo compatible le…
    Tags
    Expression
    Mutation
    temperature-tolerant mutation (D156R)
    Promoter
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    192453-plasmid-map-sequence-id-379029

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  23. pICH41331::pU6cm-pegR::EURb7

    Plasmid
    #227695
    Purpose
    Golden Gate level 0 acceptor vector for cloning altered epegRNA: altered epegRNA being driven by U6 composite promoter (pU6cm) and terminated by a dual terminator (EURb7).
    Depositor
    Jae-Yean Kim
    Article
    Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41
    Insert
    Altered epegRNA backbone with cloning sites for gRNA and RTT.
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    U6 composite
    Available since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    227695-plasmid-map-sequence-id-452432

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  25. TFORF3549

    Plasmid
    #145025
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens. This is the control vector for the collection.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    GFP
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    145025-plasmid-map-sequence-id-419572

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  27. TFORF3550

    Plasmid
    #145026
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens. This is the control vector for the collection.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    mCherry
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    145026-plasmid-map-sequence-id-422981

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  29. 7M8

    Plasmid
    #64839
    Purpose
    This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the 7M8 capsid protein.
    Depositor
    David Schaffer, John Flannery
    Article
    Dalkara et al Sci Transl Med. 2013 Jun 12;5(189):189ra
    Insert
    7M8 cap
    Use
    AAV
    Tags
    Expression
    Mutation
    7mer insertion in the AAV2 cap
    Promoter
    Available since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    64839-plasmid-map-sequence-id-342083

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  31. rAAV2/MG1.2

    Plasmid
    #184541
    Purpose
    AAV packaging plasmid, expressing Rep2 and AAV-MG1.2 capsid
    Depositor
    Minmin Luo
    Article
    Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s
    Insert
    AAV-MG1.2 capsid
    Use
    AAV; Helper vector for packaging
    Tags
    Expression
    Mutation
    changes on AAV9 capsid: K449R, Q588T; insertion o…
    Promoter
    Available since
    July 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    184541-plasmid-map-sequence-id-361941

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  32. ppyCAG_RNaseH1_WKKD

    Plasmid
    #111905
    Purpose
    Express WKKD mutated RNASEH1 in mammalian cell. The combination of three specific mutations (W43A, K59A, K60A) in the binding domain prevents the enzyme from binding to RNA/DNA hybrids
    Depositor
    Xiang-Dong Fu
    Article
    Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.
    Insert
    RNASEH1 (RNASEH1 Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Changed D 210 to N, W 43 to A, K 59 to A, K 60 to…
    Promoter
    Available since
    July 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    111905-plasmid-map-sequence-id-212872

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  33. pSIJ8

    Plasmid
    #68122
    Purpose
    Temperature sensitive plasmid for either lambda Red recombinase genes or flippase recombinase expression
    Depositor
    Alex Nielsen
    Article
    Jensen et al Sci Rep. 2015 Dec 8;5:17874. doi: 10.103
    Inserts
    flp recombinase
    rhaS
    rhaR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    rhamnose
    Available since
    Dec. 8, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    68122-plasmid-map-sequence-id-243302

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  35. pLV6-Bmal-luc

    Plasmid
    #68833
    Purpose
    Expression of Bmal1-luc in mammalian cells
    Depositor
    Steven Brown
    Insert
    Bmal1 regulatory sequences (Bmal1 Mouse)
    Use
    Lentiviral and Luciferase
    Tags
    luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    68833-plasmid-map-sequence-id-455401

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  38. pTSin EGFP

    Plasmid
    #127695
    Purpose
    Plasmid contains the entire replication competent Sindbis genome with the structural genome components replaced by EGFP in an MCS locus. See Resource Information section for annotated plasmid sequence
    Depositor
    Justin English, Bryan Roth
    Article
    English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306
    Insert
    EGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 3, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    127695-plasmid-map-sequence-id-398129

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  46. CIBN-CAAX

    Plasmid
    #79574
    Purpose
    expression of N-terminal portion of CIB1 with a C-terminal CAAX box from KRras for plasma-membrane targeting
    Depositor
    Olof Idevall-Hagren, Pietro De Camilli
    Article
    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10
    Insert
    CIBN (CIB1 Mustard Weed)
    Use
    Tags
    C-terminal CAAX-box
    Expression
    Mammalian
    Mutation
    K93A, R94A, K106A, K107A (please see depositor co…
    Promoter
    CMV
    Available since
    July 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    79574-plasmid-map-sequence-id-314173

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  48. BPK764

    Plasmid
    #65767
    Purpose
    Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Mutation
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65767-plasmid-map-sequence-id-471631

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Showing: 351 - 400 of 19133 results