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171,694 results

Showing: 351 - 400 of 171694 results

  2. TACR1-Tango

    Plasmid
    #66514
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    TACR1 (TACR1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  5. DVC_p15A_AF

    Plasmid
    #217588
    Purpose
    Destination vector with chloramphenicol resistance and p15A origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning.
    Depositor
    Wei Huang
    Article
    Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    None
    Available Since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217588-plasmid-map-sequence-id-442568

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  7. R777-E317 Hs.SOS1

    Plasmid
    #70601
    Purpose
    Gateway ORF clone of human SOS1 [NM_005633.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    SOS1 (SOS1 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70601-plasmid-map-sequence-id-358354

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  8. MF64_PB-TRE3G-6x42bs-6x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA

    Plasmid
    #176624
    Purpose
    TF B self-activation cassette in MultiFate-2.1 and MultiFate-2.3
    Depositor
    Michael Elowitz
    Article
    Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.
    Insert
    TRE3G-6x42bs-6x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA
    Use
    Synthetic Biology
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    176624-plasmid-map-sequence-id-347134

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  12. TFORF1497

    Plasmid
    #143126
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    LYL1 (LYL1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    143126-plasmid-map-sequence-id-397859

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  14. pAAV-nEF-NpHR3.3-EYFP (AAV8)

    Viral Prep
    #137151-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-nEF-NpHR3.3-EYFP (#137151). In addition to the viral particles, you will also receive purified pAAV-nEF-NpHR3.3-EYFP plasmid DNA. nEF-driven expression of NpHR3.3-EYFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    nEF
    Tags
    EYFP
    Available Since
    May 5, 2021
    Availability
    Academic Institutions and Nonprofits only

  18. TFORF0176

    Plasmid
    #141543
    Purpose
    Lentiviral vector for overexpressing the RELA transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    RELA (RELA Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141543-plasmid-map-sequence-id-276765

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  20. pLX-AS

    Plasmid
    #188870
    Purpose
    Compact T-DNA binary vector of the pLX series suitable for assembly of plant virus infectious clones and their Agrobacterium-mediated delivery to plants
    Depositor
    Fabio Pasin
    Article
    Pasin et al STAR Protoc. 2022 Dec 16;3(4):101716. do
    Insert
    none
    Use
    Synthetic Biology; Binary t-dna vector
    Expression
    Plant
    Promoter
    cauliflower mosaic virus (CaMV) 35S promoter
    Available Since
    Nov. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    188870-plasmid-map-sequence-id-377941

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  23. SmBiT-MDM2 Control Vector

    Plasmid
    #237037
    Purpose
    Express SmBiT-MDM2 Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    MDM2 (MDM2 Human)
    Tags
    SmBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237037-plasmid-map-sequence-id-471894

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  24. LgBiT-p53 Control Vector

    Plasmid
    #237036
    Purpose
    Express LgBiT-p53 Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    p53 (TP53 Human)
    Tags
    LgBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237036-plasmid-map-sequence-id-471893

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  31. MRGPRX1-Tango

    Plasmid
    #66439
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    MRGPRX1 (MRGPRX1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  34. pFP5299 ss-FIREmate-HA- KDEL-IVS-IRES- ManII-SBP- mApple-FIREtag

    Plasmid
    #216695
    Purpose
    Synchronize anterograde and retrograde trafficking of ManII between ER and Golgi (RUCH system)
    Depositor
    Franck Perez
    Article
    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s
    Inserts
    FIREmate-KDEL
    ManII-SBP-mApple-FIREtag (Man2a1 Mouse)
    Tags
    FIREtag, Streptavidin Binding Protein (SBP), and …
    Expression
    Mammalian
    Mutation
    contains only amino acids 1 to 116 of ManII
    Promoter
    CMV
    Available Since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    216695-plasmid-map-sequence-id-429706

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  36. TFORF0404

    Plasmid
    #141606
    Purpose
    Lentiviral vector for overexpressing the CTCFL transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CTCFL (CTCFL Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141606-plasmid-map-sequence-id-409169

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  37. pCMV-T7-AsCas12a-P2A-EGFP (RTW2861)

    Plasmid
    #160140
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized AsCas12a with a c-terminal NLS(nucleoplasmin), 3x HA tag, and P2A-EGFP
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41
    Insert
    human codon optimized AsCas12a with NLS(nucleoplasmin)-3xHA-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    NLS(nucleoplasmin)-3xHA-P2A-EGFP
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available Since
    Feb. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    160140-plasmid-map-sequence-id-317331

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  38. tdTurboRFP-Mito-7

    Plasmid
    #58062
    Purpose
    Localization: Mitochondria, Excitation: 553, Emission: 576
    Depositor
    Michael Davidson
    Insert
    Mito (COX8A Human)
    Tags
    tdTurboRFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 4, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  42. pCLIPf-NK1R Control Plasmid

    Plasmid
    #101125
    Purpose
    Expression of CLIP-tagged Neurokinin-1 Receptor in Mammalian cells
    Depositor
    New England Biolabs, Ana Egana
    Insert
    Neurokinin-1 Receptor, Truncated (TACR1 Human)
    Tags
    10xHis, CLIP-tag (CLIPf), and FLAG Epitope
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 27, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101125-plasmid-map-sequence-id-194114

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  47. TFORF3206

    Plasmid
    #144682
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TBR1 (TBR1 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144682-plasmid-map-sequence-id-350838

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  48. pMito-dCherry-FRB

    Plasmid
    #186573
    Purpose
    Expression of "dark MitoTrap" - an FRB-domain targeted to the mitochondria that is the same size as pMito-mCherry-FRB but is non-fluorescent - in human cells
    Depositor
    Stephen Royle
    Article
    Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:
    Insert
    Dark MitoTrap
    Expression
    Mammalian
    Mutation
    Mutation of "K70N" to render mCherry no…
    Promoter
    CMV
    Available Since
    Aug. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    186573-plasmid-map-sequence-id-368442

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Showing: 351 - 400 of 171694 results