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169,512 results

Showing: 401 - 450 of 169512 results

  2. pZac2.1 gfaABC1D-cyto-GCaMP8m

    Plasmid
    #186051
    Purpose
    AAV-mediated expression of GCaMP8m under the control of GfaABC1D promoter
    Depositor
    Loren Looger
    Insert
    jGCaMP8m
    Use
    AAV
    Tags
    6xHis
    Expression
    Mammalian
    Promoter
    GfaABC1D
    Available Since
    Aug. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    186051-plasmid-map-sequence-id-370165

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  3. qTAG-C-mNeon-Puro-TOMM20

    Plasmid
    #207790
    Purpose
    Donor template for mNeon-2A-Puro insertion into the C-terminus of the TOMM20 locus. For mitochondria visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TOMM20 (Addgene #207789)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TOMM20 Homology Arms flanking a mNeon-Puro Cassette (TOMM20 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    207790-plasmid-map-sequence-id-415639

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  4. AV0-BWS-2.0-mC-G-CL3B

    Plasmid
    #124975
    Purpose
    evaluate autophagy
    Depositor
    Kazuhiro Oka
    Insert
    mCherry-GFP-LC3B
    Use
    AAV
    Promoter
    EF1-alpha
    Available Since
    May 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    124975-plasmid-map-sequence-id-243955

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  8. TFORF1919

    Plasmid
    #141887
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    RUNX1 (RUNX1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141887-plasmid-map-sequence-id-392593

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  20. mEmerald-Desmoglein1-N-18

    Plasmid
    #54064
    Purpose
    Localization: Desmosomes, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Desmoglein1 (DSG1 Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 23, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54064-plasmid-map-sequence-id-444156

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  24. mEmerald-Peroxisomes-2

    Plasmid
    #54228
    Purpose
    Localization: Peroxisomes, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Peroximal Targeting Signal 1 (PTS1)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 10, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  28. pCB’ CbbL Y72R

    Plasmid
    #162707
    Purpose
    Carboxysome operon with point mutation to CbbL, prk; Substitution of CsoS2-binding tyrosine with arginine prevents rubisco encapsulation creating rubisco-less carboxysomes
    Depositor
    David Savage
    Article
    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1
    Insert
    pHnCB10 derived carboxysome operon, prk
    Expression
    Bacterial
    Mutation
    CbbL Y72R; Substitution of CsoS2-binding tyrosine…
    Promoter
    PLtet0-1 promoter
    Available Since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    162707-plasmid-map-sequence-id-326996

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  30. mEmerald-Sequestosome1-C-18

    Plasmid
    #54251
    Purpose
    Localization: Membrane Scaffold, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Sequestosome1 (SQSTM1 Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 10, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  31. pEGB3_alpha1 P35Sx2_GFP_Tnos (GB2980)

    Plasmid
    #160634
    Purpose
    TU for wtGFP expression driven by 2x35s promoter.
    Depositor
    Diego Orzaez
    Insert
    GFP
    Use
    Synthetic Biology
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    2x35s
    Available Since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160634-plasmid-map-sequence-id-321964

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  32. PDPN-bio-His

    Plasmid
    #52313
    Purpose
    Expresses full-length Podoplanin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    PDPN (PDPN Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52313-plasmid-map-sequence-id-80943

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  40. pY2

    Plasmid
    #218115
    Purpose
    Activity reporter backbone with an empty protease cassette (BsaI) under (lexA-box)3PminCYC1 promoter and an empty substrate cassette (BsmBI) under pGAL1 promoter.
    Depositor
    Carl Denard
    Article
    Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae
    Type
    Empty backbone
    Expression
    Bacterial and Yeast
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    218115-plasmid-map-sequence-id-431070

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  43. gCOTS-pyl

    Plasmid
    #92048
    Purpose
    This is a S. elongatus (PCC7942) shuttle vector used for recombination of the pylRS orthogonal translation system to genomic neutral site 2.
    Depositor
    Lital Alfonta
    Article
    Chemla et al Biochemistry. 2017 Apr 12. doi: 10.1021/
    Inserts
    Pyrrolysyl tRNA(cua)
    Pyrrolysyl tRNA synthetase (methanosarcina mazei)
    Use
    Cyanobacteria s. elongatus genome recombination i…
    Promoter
    LeuP (native S. elongatus promoter) and PrcbL
    Available Since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    92048-plasmid-map-sequence-id-181707

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  45. p1.1-mCherry

    Plasmid
    #162772
    Purpose
    Fluorescent reporter for CHO expression studies
    Depositor
    Ivan Vorobiev
    Article
    Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2
    Insert
    red fluorescent protein
    Expression
    Mammalian
    Mutation
    consensus Kozak sequence (GCCGCCATGG) added befor…
    Promoter
    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…
    Available Since
    Aug. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    162772-plasmid-map-sequence-id-339102

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  46. p1.2-GS-eGFP

    Plasmid
    #162771
    Purpose
    Fluorescent reporter for CHO expression studies
    Depositor
    Ivan Vorobiev
    Article
    Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2
    Insert
    enhanced green fluorescent protein
    Expression
    Mammalian
    Mutation
    consensus Kozak sequence (GCCGCCATGG) added befor…
    Promoter
    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…
    Available Since
    Aug. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    162771-plasmid-map-sequence-id-339094

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  47. R777-E284 Hs.ROCK1-nostop

    Plasmid
    #70568
    Purpose
    Gateway ORF clone of human ROCK1 [NM_005406.2] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    ROCK1 (ROCK1 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70568-plasmid-map-sequence-id-126017

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Showing: 401 - 450 of 169512 results