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Showing: 401 - 450 of 157134 results

  10. mCherry-MannII-N-10

    Plasmid
    #55074
    Purpose
    Localization: Golgi Complex, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    MannII
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    aa 1-112 of MannII
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. TFORF2292

    Plasmid
    #142012
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    HES7 (HES7 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    142012-plasmid-map-sequence-id-391436

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  22. pDule- Mj Acd A9

    Plasmid
    #197651
    Purpose
    E. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pair
    Depositor
    Ryan Mehl
    Article
    Sungwienwong et al Org Biomol Chem. 2017 May 3;15(17):3603-
    Inserts
    Mj Acd A9 synthetase
    Methanocaldococcus jannaschii tRNA
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32A, L65D, F108L, Q109E, D158S, L162T
    Promoter
    GlnS and lpp
    Available since
    April 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197651-plasmid-map-sequence-id-389657

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  26. pBEST-p15a-pBAD-UTR1-TetR-T500

    Plasmid
    #45787
    Depositor
    Richard Murray
    Insert
    Tetracycline repressor
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    July 16, 2013
    Availability
    Academic Institutions and Nonprofits only
    45787-plasmid-map-sequence-id-64258

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  33. pACYC-T7-SpCas9(BspMI/SapI/BsaI_cassettes)-T7-gRNA1 (BPK1807)

    Plasmid
    #223065
    Purpose
    Dual pT7 entry plasmid for SpCas9(6AA) library; bacterial expression plasmid with type IIS RE cassettes around D1135/S1136, G1218/E1219, R1335/T1337 (precursor to MMW94). Expresses a gRNA.
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    entry vector for human codon opt. bacterial expr. plasmid for SpCas9(6AA_NNS) library, with gRNA targeting EGFP site 1
    Use
    CRISPR
    Tags
    NLS(SV40)-3xFLAG
    Expression
    Bacterial
    Mutation
    Three regions of SpCas9 encode type IIS restricti…
    Promoter
    Dual T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223065-plasmid-map-sequence-id-447614

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  34. pAAV-syn-FLP-FRT-iGluSnFR4s-NGR-WPRE

    Plasmid
    #234453
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4s-NGR
    Use
    AAV; Flp/frt
    Tags
    IgK chain and Myc epi tag-NGR GPI anchor
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234453-plasmid-map-sequence-id-465748

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  38. pFA6a-link-ymScarletI3-URA3

    Plasmid
    #224075
    Purpose
    Yeast tagging vector to create a gene fusion with yeast optimized mScarletI3 with URA3 selection
    Depositor
    Bas Teusink
    Insert
    ymScarletI3
    Use
    Tags
    ymScarletI3
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    224075-plasmid-map-sequence-id-460510

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  39. pFA6a-link-ymScarletI3-Kan

    Plasmid
    #224077
    Purpose
    Yeast tagging vector to create a gene fusion with yeast optimized mScarletI3 with Kan selection
    Depositor
    Bas Teusink
    Insert
    ymScarletI3
    Use
    Tags
    ymScarletI3
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    224077-plasmid-map-sequence-id-460512

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  40. pM-mTagBFP2

    Plasmid
    #228615
    Purpose
    Mammalian expression of mTagBFP2 and extra insert, for MuSIC barcode construction
    Depositor
    Marc Birtwistle
    Article
    Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.
    Insert
    mTagBFP2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    We introduced some silent mutations to avoid esse…
    Promoter
    CMV
    Available since
    Jan. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    228615-plasmid-map-sequence-id-457660

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  44. pLenti-nSpCas9-APOBEC3A

    Plasmid
    #209040
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC3A is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    APOBEC3A-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209040-plasmid-map-sequence-id-413592

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  46. pBTK1050

    Plasmid
    #209540
    Purpose
    BTK Type 1 homology arm for Golden Gate assembly
    Depositor
    Jeffrey Barrick
    Article
    Lariviere et al bioRxiv 2023.09.19.558440
    Insert
    Upstream homology arm for recombination into the S. alvi wkB2 genome w/in the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    209540-plasmid-map-sequence-id-422358

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  47. pBTK1051

    Plasmid
    #209541
    Purpose
    BTK Type 5 homology arm for Golden Gate assembly
    Depositor
    Jeffrey Barrick
    Article
    Lariviere et al bioRxiv 2023.09.19.558440
    Insert
    Recombination into the S. alvi wkB2 genome within the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 2, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    209541-plasmid-map-sequence-id-421604

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  48. pLCS108

    Plasmid
    #209329
    Purpose
    Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal mCherry fusion protein.
    Depositor
    Lucia Strader
    Article
    Allen et al Res Sq. 2023 Nov 7:rs.3.rs-3558280. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    209329-plasmid-map-sequence-id-420443

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Showing: 401 - 450 of 157134 results