179,463 results
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Plasmid#107172Purposeexpresses 3xHA-tagged miniTurbo in the mammalian nucleusDepositorInsertminiTurbo (BirA mutant)
ExpressionMammalianMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Available sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-FLEX-tdTomato (AAV PHP.eB)
Viral prep#28306-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV-FLEX-tdTomato (#28306). In addition to the viral particles, you will also receive purified pAAV-FLEX-tdTomato plasmid DNA. CAG-driven, Cre-dependent tdTomato expression control. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagstdTomato (Cre-dependent)Available sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-C1-PLCdelta-PH
Plasmid#21179Purposeencodes a PI(4,5)P2 lipid selective PH domain that can be used as a fluorescent translocation biosensor to monitor changes or local differences in the concentration of plasma membrane PI(4,5)P2 lipidsDepositorInsertPLCdelta (PLCD1 Human)
TagsGFPExpressionMammalianMutationPH domain of human PLC delta, amino acids Met1–Il…Available sinceJuly 10, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV_ABEmax_P2A_GFP
Plasmid#112101PurposeA:T-to-G:C base editingDepositorInsertABEmax_P2A_GFP
UseCRISPRExpressionMammalianPromoterCMVAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti6/V5-p53_wt p53
Plasmid#22945Purpose3rd generation lentiviral vector encoding human p53 with a C-terminal V5 tagDepositorPublicationAvailable sinceJan. 27, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-DsRed
Plasmid#11151PurposeMammalian expression vector for expression of DsRedDepositorInsertDsRed2 from Discosoma sp
ExpressionMammalianAvailable sinceApril 13, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRSETa mEos2
Plasmid#20341Purposebacterial expression of an EosFP variant, mEos2 (a photoconvertible fluorescent protein)DepositorInsertmEos-2
TagsHisExpressionBacterialAvailable sinceMarch 16, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pENN.AAV.hSyn.Cre.WPRE.hGH (AAV9)
Viral prep#105553-AAV9PurposeReady-to-use AAV9 particles produced from pENN.AAV.hSyn.Cre.WPRE.hGH (#105553). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.Cre.WPRE.hGH plasmid DNA. hSyn-driven Cre expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDD162 (Peft-3::Cas9 + Empty sgRNA)
Plasmid#47549PurposeCas9 + sgRNA plasmid that can be modified to cleave any Cas9 target site in the C. elegans genome.DepositorInsertsCas9
Empty sgRNA
UseCRISPRTagsHA and NLSExpressionWormMutationCodon optimized and with synthetic introns for C.…PromoterU6 and eef-1A.1 (eft-3)Available sinceSept. 9, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SV40 1: pBSSVD2005
Plasmid#21826DepositorInsertSV40 Large T (SV40gp6 simian virus)
ExpressionMammalianMutationlikely expresses aa109-708 of the large T antigenAvailable sinceSept. 14, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PX552
Plasmid#60958PurposepAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei.DepositorInsertsU6_(SpaI)_sgRNA
Syn_EGFP-KASH
UseAAV, CRISPR, and Mouse TargetingExpressionMammalianPromoterSynapsin and U6Available sinceNov. 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBabe puro HA PIK3CA H1047R
Plasmid#12524PurposeRetroviral expression of HA tagged phosphoinositide 3-kinase H1047R (Tumor-derived allele)DepositorInsertPIK3CA H1047R (PIK3CA Human)
UseRetroviralTagsHAExpressionMammalianMutationH1047R. Tumor-derived allele.Available sinceAug. 18, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ph7SK-gRNA
Plasmid#53189PurposeExpresses the S. pyogenes sgRNA from the 7SK promoterDepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterhuman 7SKAvailable sinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDGM6
Plasmid#110660PurposeAdeno-Associated Virus (AAV) serotype 6; contains the AAV6 cap genes, AAV2 rep genes, and adenovirus helper genes. Good for in vivo transduction of muscle, lung, liver and other tissues.DepositorInsertAAV6 cap genes, AAV2 rep genes, adenovirus helper genes
UseAAVAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-EGFP-C4-Nrf2
Plasmid#21549DepositorInsertnuclear factor (erythroid-derived 2)-like 2 (NFE2L2 Human)
TagsEGFP and MycExpressionMammalianAvailable sinceJuly 1, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMSCV-F-del Casp9.IRES.GFP
Plasmid#15567DepositorInsertFKBP12(V36)-p30Caspase9 (FKBP1A Human)
UseRetroviralTagsFKBP12 and HA epitopeExpressionMammalianMutationFKBP12 F36 to V Truncated Caspase9 (135-416; lac…Available sinceAug. 22, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-flex-taCasp3-TEVp (AAV5)
Viral prep#45580-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-flex-taCasp3-TEVp (#45580). In addition to the viral particles, you will also receive purified pAAV-flex-taCasp3-TEVp plasmid DNA. EF1a-driven, Cre-dependent, bicicstronic expression of designer pro-taCasp3 and TEVp. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS185 CMV-Cre
Plasmid#11916PurposeMammalian expression of Cre recombinase driven by the human cytomegalovirus IE promoterDepositorPublicationAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
xCas9(3.7)-ABE(7.10)
Plasmid#108382PurposeMammalian expression vector for xCas9(3.7)-ABEDepositorInsertxCas9(3.7)-ABE(7.10)
TagsNLSExpressionMammalianMutationA262T, R324L, S409I, E480K, E543D, M694I, E1219VAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCFD3-dU6:3gRNA
Plasmid#49410Purposeexpression of gRNA under control of the Drosophila U6:3 promoterDepositorInsertdU6-3:gRNA
UseCRISPRExpressionInsectPromoterdU6-3Available sinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MoClo Yeast Toolkit
Plasmid kit#1000000061PurposeCollection of standardized parts for hierarchical assembly of single and multi-gene constructs for S. cerevisiae expression.DepositorApplicationCloning and Synthetic BiologyVector typeYeast ExpressionCloning typeGolden Gate (MoClo)Available sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEP4 E02S ET2K
Plasmid#20927Purposeused in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; SV40LT and Klf4DepositorPublicationAvailable sinceMay 20, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3 MDM2 WT
Plasmid#16233DepositorInsertMDM2 (MDM2 Human)
ExpressionMammalianAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pU6-Sp-pegRNA-HEK3_CTT_ins
Plasmid#132778PurposeS. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editingDepositorInsertHEK3_CTT_ins pegRNA
ExpressionMammalianMutationSee manuscriptPromoterU6Available sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV R-CEPIA1er
Plasmid#58216PurposeRed fluorescent indicator for calcium signaling in the endoplasmic reticulumDepositorInsertR-CEPIA1er
TagsmycExpressionMammalianMutationR-GECO1 E300D M305L D329N F332I E336D N346K F361W…PromoterCMVAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSpCas9n(BB) (PX460)
Plasmid#48873PurposeCas9n (D10A nickase mutant) from S. pyogenesDepositorInserthSpCas9n
UseCRISPRTags3XFLAGExpressionMammalianMutationCas9 D10A nickase mutantPromoterCbhAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMSCV PIG (Puro IRES GFP empty vector)
Plasmid#21654PurposeRetroviral backbone for cloning and gene expression. Select with puromycin or GFP.DepositorPublicationTypeEmpty backboneUseRetroviralTagsGFP and IRESExpressionMammalianAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Lox-Stop-Lox TOPO
Plasmid#11584PurposeCre-dependent activation of gene of interest by removing Stop sequences; Puro selection.DepositorInsertloxP-STOP-loxP
UseCre/LoxExpressionMammalianAvailable sinceJune 22, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 (AAV PHP.eB)
Viral prep#105540-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 (#105540). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 plasmid DNA. Expression of EGFP-Cre from hSyn promoter. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFPAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
MS2-P65-HSF1_GFP
Plasmid#61423PurposeExpresses the MS2-P65-HSF1 activator helper complex with a 2A GFP. 3rd generation lentiviral vector.DepositorInsertMS2(N55K)-P65-HSF1_2A_GFP (HSF1 Human, Synthetic)
UseCRISPR and LentiviralExpressionMammalianMutationN55K in MS2PromoterEF1AAvailable sinceDec. 15, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.CAG.GCaMP6s.WPRE.SV40 (AAV9)
Viral prep#100844-AAV9PurposeReady-to-use AAV9 particles produced from pAAV.CAG.GCaMP6s.WPRE.SV40 (#100844). In addition to the viral particles, you will also receive purified pAAV.CAG.GCaMP6s.WPRE.SV40 plasmid DNA. CAG-driven GCaMP6s calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
C321.ΔA.exp
Bacterial strain#49018PurposeThis is C321.DA with mutS reverted and the lambda prophage removed; it exhibits lesser spontaneous mutagenesis and can be grown at 37 CDepositorBacterial resistanceBleocin (Zeocin)SpeciesEscherichia coliAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBabe-Puro-BRAF-V600E
Plasmid#15269PurposeRetroviral expression of BRAF V600E (the V600E mutation has been found in cancer patients, confers increased kinase activity, and is transforming)DepositorPublicationInsertBRAF (BRAF Human)
UseRetroviralTagsHis and MycExpressionMammalianMutationValine 600 to Glutamate (mutation has been found …Available sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mito-PAGFP
Plasmid#23348PurposeExpresses a mitochondrial matrix-targeted photoactivable GFP that enables detection and quantification of organelle fusion in living cells.DepositorInsertPAGFP
TagsmitoExpressionMammalianAvailable sinceApril 9, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGP-CMV-NES-jRGECO1a
Plasmid#61563PurposeRed fluorescent calcium sensor proteinDepositorInsertNES-jRGECO1a
Tagsnuclear export signalExpressionMammalianPromoterCMVAvailable sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-TLR4-YFP
Plasmid#13018DepositorAvailable sinceNov. 3, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pC016 - LwCas13a guide expression backbone with U6 promoter
Plasmid#91906PurposeBackbone for cloning LwCas13a guides under U6 promoterDepositorInsertLwCas13a Guide site
ExpressionMammalianAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CAG-GFP (AAV9)
Viral prep#37825-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsGFPAvailable sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCh-Drp1
Plasmid#49152Purposemammalian expression of Drp1 fused to mCherryDepositorAvailable sinceNov. 18, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3/Myc-DNMT1
Plasmid#36939PurposeMammalian expression of human DNMT1 with myc tagDepositorAvailable sinceJune 22, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-Parkin
Plasmid#23956PurposeMammalian expression of human Park2 fused to mCherryDepositorAvailable sinceMarch 24, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMVHA hEZH2
Plasmid#24230PurposeMammalian expression of human EZH2 with HA tagDepositorAvailable sinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDEST-HisMBP
Plasmid#11085DepositorTypeEmpty backboneTags6xHis and MBPExpressionBacterialPromoterTac (lactose/IPTG inducible)Available sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCh-Sec61 beta
Plasmid#49155Purposeexpression of Sec61 beta fused to mCherry, used as a general ER markerDepositorAvailable sinceNov. 20, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PGC-1 alpha promoter 2kb luciferase
Plasmid#8887DepositorAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MoClo Tool Kit
Plasmid kit#1000000044PurposeCollection of standardized genetic modules that can be used for hierarchical assembly of multi-gene expression constructs.DepositorApplicationCloning and Synthetic BiologyVector typeEukaryotic ExpressionCloning typeGolden Gate (MoClo)Available sinceApril 8, 2014AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pcDNA3-EGFP-RhoA-T19N
Plasmid#12967DepositorAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mKeima-Red-Mito-7
Plasmid#56018PurposeLocalization: Mitochondria, Excitation: 440, Emission: 620DepositorAvailable sinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMS2-GFP
Plasmid#27121DepositorPublicationInsertMS2
TagsEGFP, HA, and SV40 NLSExpressionMammalianAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMSCV-IRES-mCherry FP
Plasmid#52114Purposea bicistronic IRES-mCherry containing retroviral vectorDepositorTypeEmpty backboneUseRetroviralTagsIRES-mCherryExpressionMammalianAvailable sinceMay 9, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by