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171,575 results

Showing: 351 - 400 of 171575 results

  1. pSupAR-MbPylRS(DiZPK)

    Plasmid
    #91705
    Purpose
    express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in E. coli to incorporate photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into an in-frame amber codon site on a protein of interest
    Depositor
    P. Chen
    Article
    Zhang et al Nat Chem Biol. 2011 Sep 4;7(10):671-7. d
    Inserts
    pylS
    pylT
    Expression
    Bacterial
    Mutation
    L274A, C313S
    Available Since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    91705-plasmid-map-sequence-id-183278

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  3. pET22b_PstS_2

    Plasmid
    #78199
    Purpose
    Expression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor
    Depositor
    Martin Webb
    Article
    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62
    Insert
    pstS (A17C,A197C)
    Expression
    Bacterial
    Mutation
    A17C; A197C (please see depositor comment below)
    Promoter
    T7
    Available Since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    78199-plasmid-map-sequence-id-146049

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  4. CMV-GFP-Kir2.1

    Plasmid
    #107184
    Purpose
    mammalian expression of Kir2.1
    Depositor
    Bikash Pattnaik
    Insert
    KCNJ2 (KCNJ2 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    107184-plasmid-map-sequence-id-203927

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  8. iRFP-H2A

    Plasmid
    #158691
    Purpose
    This plasmid encodes an iRFP fused to H2A histone nuclear marker. It has CMV promoter driving the expression of iRFP fused to H2A. It has no selection markers
    Depositor
    Ravid Straussman
    Article
    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:
    Insert
    iRFP670
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    158691-plasmid-map-sequence-id-311047

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  9. TFORF2746

    Plasmid
    #144351
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    HELZ2 (PRIC285 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144351-plasmid-map-sequence-id-404198

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  15. pLV-EBFP2-nuc

    Plasmid
    #36085
    Depositor
    Pantelis Tsoulfas
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Replace EYFP of the vector pEYFP-Nuc from Clontec…
    Available Since
    June 1, 2012
    Availability
    Academic Institutions and Nonprofits only
    36085-plasmid-map-sequence-id-44618

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  19. Talin-TS

    Plasmid
    #83376
    Purpose
    FRET probe for measuring tension on Talin molecule
    Depositor
    Martin Schwartz
    Article
    Kumar et al J Cell Biol. 2016 May 9;213(3):371-83. d
    Insert
    Talin1 (Tln1 Mouse)
    Use
    Retroviral
    Tags
    EGFP-F40-tagRFP (tension sensor module)
    Expression
    Mammalian
    Mutation
    Tension sensor module is inserted between V447 an…
    Promoter
    CMV
    Available Since
    Oct. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    83376-plasmid-map-sequence-id-158760

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  20. pExp-MBP

    Plasmid
    #112568
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and MBP fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis-MBP and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112568-plasmid-map-sequence-id-217224

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  23. PB-CT3G-CERP2-MG

    Plasmid
    #175503
    Purpose
    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)
    Depositor
    George Church
    Article
    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    TRE3G-mCMV
    Available Since
    Oct. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    175503-plasmid-map-sequence-id-345096

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  32. SIN-cPPT-PGK-mitoGO-ATeam2-WPRE

    Plasmid
    #245083
    Purpose
    Mitochondrial ATP biosensor
    Depositor
    Nicole Déglon
    Insert
    mitoGO-ATeam2
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    Mouse PGK
    Available Since
    Feb. 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    245083-plasmid-map-sequence-id-499134

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  39. pET22b_PstS_3

    Plasmid
    #78200
    Purpose
    Expression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensor
    Depositor
    Martin Webb
    Article
    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62
    Insert
    pstS (A17C,I76G,A197C)
    Expression
    Bacterial
    Mutation
    A17C,I76G,A197C (please see depositor comment bel…
    Promoter
    T7
    Available Since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    78200-plasmid-map-sequence-id-146050

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  45. pLenti-C-Myc-DDK-IRES-Puro HNRNPQ

    Plasmid
    #200729
    Purpose
    Lentiviral expression of Flag-Tagged human HNRNPQ
    Depositor
    Claudio Alarcón
    Insert
    HNRNPQ (SYNCRIP Human)
    Use
    Lentiviral
    Tags
    Myc-DDK from backbone plasmid
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    200729-plasmid-map-sequence-id-407124

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  47. TFORF1759

    Plasmid
    #143845
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ALYREF (ALYREF Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    143845-plasmid-map-sequence-id-396272

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  50. CA-RIT-NFAT1 (IRES-GFP)

    Plasmid
    #85181
    Purpose
    Constitutively active NFAT1, mutated to interfere selectively with the NFAT:AP-1 interaction. Co-expresses EGFP for selection
    Depositor
    Anjana Rao
    Article
    Martinez et al Immunity. 2015 Feb 6. pii: S1074-7613(15
    Insert
    CA-RIT-NFAT1 (Nfatc2 Mouse)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Amino acids 1-3 removed; CA: PASSGSSASF mutated t…
    Available Since
    April 12, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 351 - 400 of 171575 results