159,238 results
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Plasmid#58124PurposeLocalization: Mitochondria, Excitation: 554, Emission: 581DepositorInsertPDHA1 (PDHA1 Human)
UseTagstdTomatoExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV2/9 REP-AAP-ΔCAP
Plasmid#205991PurposeRep and AAP protein expression for AAV CREATE librariesDepositorInsertAAV9 Rep and AAP proteins for CREATE libraries
UseAAVTagsExpressionMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMBP-LbCas12a
Plasmid#113431PurposeExpresses 10xHis-MBP fused to LbCas12aDepositorHas ServiceCloning Grade DNAInsertLbCas12a
UseTagsMBPExpressionBacterialMutationPromoterT7Available sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
circRNA-synIRES-R25-NanoLuc
Plasmid#188116PurposeTemplate plasmid to transcribe circular RNA encoding NanoLucDepositorInsertNanoLuc
UseLuciferaseTagsExpressionMammalianMutationPromoterT7Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lamp1-mTurquoise2
Plasmid#98828PurposeIn vivo visualization of lysosomes (can be used for colocalization studies)DepositorArticleInsertLamp1
UseTagsmTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF3549
Plasmid#145025PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens. This is the control vector for the collection.DepositorInsertGFP
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-Gal3
Plasmid#85662PurposeFluorescent reporter for lysosomal damageDepositorInsertLGALS3 (LGALS3 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(+)-CMV-bArrestin2-TEV
Plasmid#107245PurposeVector for preparation of stable cell lines expressing Barrestin2-TEV fusion protein in lieu of the HTLA cell line.DepositorInsertARRB2
UseTagsTEV(NIA), tobacco etch virus NIAExpressionMammalianMutationPromoterCMVAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
P4-PE
Plasmid#211375PurposePackaging plasmid for v3b PE-eVLP productionDepositorInsertP4-PE
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Hygro
Plasmid#139462PurposeLentiviral vector with gRNA scaffold and hygromycin selectable markerDepositorInsertno sgRNA inserted; resistance gene: hygroR
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
gag-P3-pol
Plasmid#211374PurposePackaging plasmid for v3b PE-eVLP productionDepositorInsertMMLVgag-P3-pol
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
mito-mStayGold_pTwistCMVBetaGlobWPRENeo
Plasmid#221898PurposeExpresses mStayGold targeted to the mitochondriaDepositorInsertcox8-mStayGold
UseTagsCox8ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
p5xATF6-GL3
Plasmid#11976PurposeReporter gene with five ATF6 sites that is highly sensitive to ER stressDepositorInsert5xATF6 binding site
UseTagsLuc and c-fos promoterExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_cAMPFIRE-M
Plasmid#182280PurposeMammalian expression of the cAMP sensor cAMPFIRE-M under a CMV promotor.DepositorInsertcAMPFIRE-M (cAMP sensor)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
HDM_VSV_G
Plasmid#204156PurposeVSV-G expression plasmidDepositorInsertVSV-G
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-pBpF
Plasmid#31190PurposetRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-benzoyl-l-phenylalanine into proteins in E coli in response to the amber codon, TAG.DepositorInsertM.j. p-benzoylphenylalanine RS (2 copies +tRNA)
UseTagsExpressionBacterialMutationY32G, E107P, D158T, I159SPromoterAvailable sinceDec. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6-sgRNA-CMV-GFP
Plasmid#85451PurposeExpress sgRNA in mammalian cellsDepositorInsertpCMV-EGFP
UseAAVTagsExpressionMutationPromoterpCMV-EGFPAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Tau(WT)
Plasmid#187023PurposeExpresses EGFP tagged human 2N4R tau (WT) in mammalian cells with CMV promoterDepositorInsertMAPT (MAPT Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
EFSp-GFP-YAP (5SA)
Plasmid#174170PurposeLentiviral vector to constitutively express overactive YAP (5 LATS phosporylation sites mutated to Ala) under control of the EFS promoter.DepositorInsertYAP (YAP1 Human)
UseLentiviralTags3x FLAG tagExpressionMammalianMutationFive LATS phosphorylation sites (S61, S109, S127,…PromoterEFSAvailable sinceSept. 28, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti_EF1a_TauFRET_3xKQ_wpre
Plasmid#207522PurposeLentiviral construct for the expression of a FRET-based reporter for tau seeding in mammalian cellsDepositorInsertTauFRET_P301L_3xKQ
UseLentiviralTagsNeonGreen and mTurquoise2ExpressionMutationP301L; K311Q; K317Q; K321QPromoterEF1aAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1-GfaABC1D-Rpl22-HA
Plasmid#111811PurposeExpress Rpl22HA in astrocytesDepositorInsertRibosomal protein L22 (Rpl22 Mouse)
UseTagsHAExpressionMutationPromoterAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
C1(1-29)-miniTurbo-V5_pLX304
Plasmid#107176Purposeexpresses V5-tagged miniTurbo on the mammalian ER membrane, lentiviral vectorDepositorInsertminiTurbo (BirA mutant)
UseLentiviralTagsExpressionMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …PromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-M-puro
Plasmid#125839Purposemodifying MCS, replacing EcoRI to BamHI region with BamHI and EcoRIDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/mStayGold(c4)=UtrCH
Plasmid#212020PurposeFor filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ‘=’).DepositorInsertUtrCH
UseTagsmStayGold(c4)ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-hM4D(Gi)-mCherry
Plasmid#50475PurposeGi-coupled hM4D DREADD fused with mCherry under the control of human synapsin promoter.DepositorHas ServiceAAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InserthM4D(Gi)-mCherry
UseAAVTagsmCherryExpressionMutationSee supplemental documents for DREADD mutationsPromoterhuman Synapsin 1Available sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_cAMPFIRE-L
Plasmid#182279PurposeMammalian expression of the cAMP sensor cAMPFIRE-L under a CMV promotor.DepositorInsertcAMPFIRE-L (cAMP sensor)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_iPE-C_P2A_Csy4
Plasmid#232428PurposeExpression plasmid for iPE-C prime editor RNPs with additional Csy4 protectionDepositorInsertiPE-C–P2A–Csy4
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET MBP His6 LIC cloning vector (2Cc-T)
Plasmid#37237DepositorTypeEmpty backboneUseTagsHis6 and MBPExpressionBacterialMutationPromoterT7Available sinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pmTurquoise2-LAMP1
Plasmid#110948PurposeExpresses LAMP1 fused to mTurquoise2DepositorInsertLysosomal-associated membrane protein 1 (LAMP1 Human)
UseTagsmTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFX-Tom20-mCherry
Plasmid#174188PurposeExpress mCherry with mitochondria-targeting sequence in mammalian cellsDepositorInsertmCherry with mitchondria-targeting sequence
UseTagsthe mitochondria-targeting sequence of TOM20ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ERT2-PhoCl-Gal4VP16-PhoCl-ERT2
Plasmid#87693PurposeExpress ERT2-PhoCl-Gal4VP16-PhoCl-ERT2 in mammalian cellsDepositorInsertERT2-PhoCl-Gal4VP16-PhoCl-ERT2
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET His10 MBP Asn10 TEV LIC cloning vector (2CT-10)
Plasmid#55209DepositorTypeEmpty backboneUseTags10xAsn, 10xHis, MPB, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-Bsd-CMV>tTS/rtTA:T2A:{cymR}
Plasmid#236251PurposeBlasticidin selected lentiviral vector with Tet-On regulatory proteins and the cumate repressor, for use with dual-inducible CymR-CuO systemDepositorInsertstTS/rtTA
cymR
UseLentiviralTagsExpressionMammalianMutationrTS and rtTA_M2 linked by T2APromoterCMVAvailable sinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
Mito-Dendra2
Plasmid#238077PurposeExpresses photoactivable fluorescent reporter for visualizing mitochondrial networkDepositorInsertDendra2
UseTagsMitoExpressionMammalianMutationPromoterCMVAvailable sinceJune 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
p246 eSpCas9_2gRNAs_mH11
Plasmid#164854PurposegRNA vector for targeting mouse H11 locusDepositorInsertgRNAs for targeting mouse H11 locus
UseTagsExpressionMammalianMutationPromoterU6Available sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL4_CRE-CMVmin-luc2
Plasmid#194384PurposeReporter plasmid for cAMP/Calcium assays; firefly luciferase reporter gene driven by 6x clustered CRE binding sites linked to a minimal CMV promoterDepositorInsert6xCRE-CMVmin-luc2
UseTagsExpressionMammalianMutationPromoterCMV minimalAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-UbC-rtTA-Ngn2:2A:EGFP
Plasmid#127288Purposeall-in-one inducible LV expression construct for Ngn2:2A:EGFP (aka. UNG; for direct neuronal conversion)DepositorInsertNgn2:2A:Ascl1 (NEUROG2 Human)
UseLentiviralTagsNgn2 and EGFP linked via 2AExpressionMammalianMutationPromoterAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Oct3/4
Plasmid#13366PurposeRetroviral expression of mouse Oct3/4DepositorInsertPOU domain, class 5, transcription factor 1 (Pou5f1 Mouse)
UseRetroviralTagsExpressionMammalianMutationNonePromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pKL2299A
Plasmid#222105PurposeA ternary vir helper plasmid that carries Agrobacterium tumefaciens virulence genes for enhanced plant genetic transformation.DepositorInsertvirA
UseTagsExpressionBacterial and PlantMutationPromotervirA promoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-6xHis-MBP-TEV site-TadA8.20
Plasmid#194702PurposeExpresses N-terminal MBP fused TadA8.20 in bacteriaDepositorInsertTadA8.20
UseTags6xHis and MBPExpressionBacterialMutationmutations in TadA are described in the paperPromoterT7 promoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MMLVgag-3xNES-Cas9
Plasmid#181752PurposeMMLV-Gag fused to 3x NES-SpyCas9 with the TSTLLMENSS cleavage site in between NES and Cas9. For production of virus like particles with Cas9 nuclease RNP cargo.DepositorInsertMMLV-Gag-3xNES-Cas9 (Spy)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo FlucDM EGFP
Plasmid#90172PurposeEGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains double mutationDepositorInsertFLuc EGFP
UseTagsExpressionMammalianMutationR188Q, R261Q in FLucPromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX_DSB_Spectrum-V1
Plasmid#192474PurposeLentiviral DSB Spectrum V1 Reporter SystemDepositorInsertBFP1.2, incompleteGFP
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
mcherry-DN KASH
Plasmid#125553Purposemcherry labeled DN-KASHDepositorInsertSYNE1 (SYNE1 Human)
UseTagsmcherryExpressionMammalianMutationTruncated form: only the KASH domain of nesprin-…PromoterCMVAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
T7opt in pCAGGS
Plasmid#65974PurposeHuman codon-optimized T7 polymeraseDepositorInsertT7-opt
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
hFerritinH
Plasmid#122652PurposeExpression of hFerritinH in bacterial cellsDepositorInsertferritin heavy chain 1 (FTH1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo Fluc EGFP
Plasmid#90170PurposeEGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteinsDepositorInsertFLuc EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-DIO-ATLASsnFlp
Plasmid#232352PurposeAnterograde transsynaptic tracer protein to express in presynaptic glutamatergic neurons that express Cre, Flp payload. Must use with AAV8-fDIO-Reporter viruses infected in postsynaptic cells.DepositorInsertATLASsnFlp
UseAAVTagsALFA-TagExpressionMutationPromoterhSynAvailable sinceMay 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG-VSVL
Plasmid#64085PurposeExpression vector for Vesicular Stomatitis Virus Indiana strain polymerase geneDepositorInsertVSV polymerase
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only