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177,145 results

Showing: 851 - 900 of 177145 results

  3. mRFP-Rab7

    Plasmid
    #14436
    Purpose
    Mammalian expression of Rab7 fused to mRFP. Used for localization to the late endosome, and other early endosomal vacuoles.
    Depositor
    Ari Helenius
    Publication
    Vonderheit et al PLoS Biol. 2005 Jul . 3(7):e233.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Rab 7 (RAB7A Canis familialis)
    Tags
    mRFP
    Expression
    Mammalian
    Mutation
    3 silent mutations near the C-terminus
    Available since
    March 23, 2007
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    37 Articles
    14436-plasmid-map-sequence-id-229535

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  4. pAAV2/rh10

    Plasmid
    #112866
    Purpose
    AAV packaging plasmid expressing Rep/Cap genes
    Depositor
    James M. Wilson
    Insert
    Rep2/Cap-rh10
    Use
    AAV
    Promoter
    Truncated P5
    Available since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    9 Articles
    112866-plasmid-map-sequence-id-339914

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  24. 5HRE/GFP

    Plasmid
    #46926
    Purpose
    hypoxia-responsive enhanced green fluorescent protein (EGFP)-based system
    Depositor
    Martin Brown, Thomas Foster
    Publication
    Vordermark et al Neoplasia. 2001 Nov-Dec;3(6):527-34.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    5X HRE of VEGF (VEGFA Human)
    Tags
    d2EGFP
    Expression
    Mammalian
    Mutation
    five copies of a 35-bp fragment from the hypoxia-…
    Promoter
    EF-1α promoter
    Available since
    Aug. 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    25 Articles
    46926-plasmid-map-sequence-id-177232

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  29. pUC57-mitoLbNOX

    Plasmid
    #74448
    Purpose
    This plasmid contains human codon optimized sequence of mitoLbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.
    Depositor
    Vamsi Mootha
    Publication
    Titov et al Science. 2016 Apr 8;352(6282):231-5. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mitoLbNOX
    Tags
    Flag tag with linker and mitochondrial targeting …
    Mutation
    Human codon optimized
    Available since
    June 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    13 Articles
    74448-plasmid-map-sequence-id-203966

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  30. pdCas9-DNMT3A-EGFP

    Plasmid
    #71666
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Publication
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Synthetic, S. pyogenes, Human)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    29 Articles
    71666-plasmid-map-sequence-id-129527

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  32. pet30b-7M SrtA

    Plasmid
    #51141
    Purpose
    expression plasmid for C-terminal his tagged, calcium independent S.aureus SrtA with enhanced catalytic activity
    Depositor
    Hidde Ploegh
    Insert
    S.aureus SrtA 7M
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    P94R, E105K, E108Q, D160N, D165A, K190E, K196T
    Promoter
    T7
    Available since
    Feb. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    20 Articles
    51141-plasmid-map-sequence-id-211598

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  43. FokI-dCas9

    Plasmid
    #52970
    Purpose
    Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cells
    Depositor
    David Liu
    Publication
    Guilinger et al Nat Biotechnol. 2014 Apr 25. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Fok1 fused to dCas9
    Use
    CRISPR
    Tags
    Fok1 nuclease (homodimeric wildtype) and NLS
    Expression
    Mammalian
    Mutation
    D10A and H840A in Cas9
    Promoter
    CMV
    Available since
    May 21, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    52970-plasmid-map-sequence-id-82408

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Showing: 851 - 900 of 177145 results