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177,145 results

Showing: 201 - 250 of 177145 results

  5. MCS-13X Linker-BioID2-HA

    Plasmid
    #80899
    Purpose
    To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a flexible 25nm GS linker are present upstream of BioID2 and HA tags.
    Depositor
    Kyle Roux
    Publication
    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15
    Type
    Empty backbone
    Tags
    HA and humanized A. aeolicus BioID2 with R40G
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    22 Articles
    80899-plasmid-map-sequence-id-181185

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  8. MLM3636

    Plasmid
    #43860
    Purpose
    guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence, uses U6 promoter
    Depositor
    Keith Joung
    Insert
    None
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    March 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    144 Articles
    43860-plasmid-map-sequence-id-194368

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  9. pEVOL-pAzF

    Plasmid
    #31186
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAG
    Depositor
    Peter Schultz
    Publication
    Chin et al J Am Chem Soc. 2002 Aug 7;124(31):9026-7
    Insert
    M.j. p-azidohenylalanine RS (2 copies +tRNA)
    Expression
    Bacterial
    Mutation
    Y32T, E107N, D158P, I159L, L162Q, D286R
    Available since
    Dec. 9, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    52 Articles
    31186-plasmid-map-sequence-id-334781

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  10. pPSU1

    Plasmid
    #89439
    Purpose
    High copy number plasmid 1 to prepare Penn State DNA molecular weight ladders
    Depositor
    Song Tan
    Publication
    Henrici et al Sci Rep. 2017 May 26;7(1):2438. doi: 10.
    Inserts
    500 bp EcoRV fragment
    1000 bp EcoRV fragment
    1500 bp EcoRV fragment
    2000 bp EcoRV fragment
    500 bp PstI fragment
    700 bp PstI fragment
    800 bp PstI fragment (EHMT2 Human)
    900 bp PstI fragment
    1000 bp PstI fragment (BMI1 Human)
    2000 bp PstI fragment
    Expression
    Bacterial
    Available since
    April 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    89439-plasmid-map-sequence-id-190157

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  13. pCMV-PEmax-P2A-hMLH1dn

    Plasmid
    #174828
    Purpose
    Mammalian expression of SpCas9 PEmax prime editor with P2A-human MLH1dn (codon optimized)
    Depositor
    David Liu
    Publication
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PEmax-P2A-hMLH1dn
    Tags
    SV40 bpNLS and c-Myc NLS
    Expression
    Mammalian
    Mutation
    Detailed in manuscript; deletion of residues 754-…
    Promoter
    CMV
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    28 Articles
    174828-plasmid-map-sequence-id-357934

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  15. pPSU2

    Plasmid
    #89566
    Purpose
    High copy number plasmid 2 to prepare Penn State DNA molecular weight ladders
    Depositor
    Song Tan
    Publication
    Henrici et al Sci Rep. 2017 May 26;7(1):2438. doi: 10.
    Inserts
    750 bp EcoRV fragment
    3000 bp EcoRV fragment
    4000 bp EcoRV fragment
    50 bp PstI fragment
    100 bp PstI fragment
    200 bp PstI fragment
    300 bp PstI fragment
    400 bp PstI fragment
    500 bp PstI fragment
    600 bp PstI fragment
    1500 bp Psti fragment
    4100 bp PstI fragment
    Expression
    Bacterial
    Available since
    April 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    89566-plasmid-map-sequence-id-177206

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  20. pWPXL

    Plasmid
    #12257
    Purpose
    2nd generation lentiviral transfer plasmid
    Depositor
    Didier Trono
    Type
    Empty backbone
    Use
    Cre/Lox and Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Available since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    96 Articles
    12257-plasmid-map-sequence-id-237089

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  27. mCherry2-C1

    Plasmid
    #54563
    Purpose
    Localization: C1 Cloning Vector, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mCherry2
    Expression
    Mammalian
    Available since
    June 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    50 Articles
    54563-plasmid-map-sequence-id-310151

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  28. MSCV-IRES-GFP

    Plasmid
    #20672
    Purpose
    Depositor
    Tannishtha Reya
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    IRES EGFP
    Expression
    Mammalian
    Available since
    Aug. 28, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    94 Articles
    20672-plasmid-map-sequence-id-203241

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  31. pcDNA3-Clover

    Plasmid
    #40259
    Purpose
    GFP with robust performance in protein fusions and useful as FRET donor for mRuby2 in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap
    Depositor
    Michael Lin
    Publication
    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Clover GFP
    Expression
    Mammalian
    Promoter
    CMV IE
    Available since
    Sept. 24, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    40 Articles
    40259-plasmid-map-sequence-id-215680

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  34. pCMVR8.74

    Plasmid
    #22036
    Purpose
    2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259)
    Depositor
    Didier Trono
    Insert
    gag pol tat rev
    Expression
    Mammalian
    Available since
    Oct. 14, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    253 Articles
    22036-plasmid-map-sequence-id-339901

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  35. CROPseq-Guide-Puro

    Plasmid
    #86708
    Purpose
    Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.
    Depositor
    Christoph Bock
    Publication
    Datlinger et al Nat Methods. 2017 Jan 18. doi: 10.1038/n
    Insert
    EF1a-Puro-WPRE-hU6-gRNA
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    hU6
    Available since
    Feb. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    68 Articles
    86708-plasmid-map-sequence-id-317051

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  37. G-CaMP3

    Plasmid
    #22692
    Purpose
    a single-wavelength GCaMP2-based genetically encoded calcium indicator
    Depositor
    Loren Looger
    Publication
    Tian et al Nat Methods. 2009 Dec;6(12):875-81.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    G-CaMP3
    Expression
    Mammalian
    Mutation
    N363D mutation (increased fluorescence)
    Available since
    Nov. 13, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    72 Articles
    22692-plasmid-map-sequence-id-241043

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  38. pLKO.1 neo

    Plasmid
    #13425
    Purpose
    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses neomycin for selection.
    Depositor
    Sheila Stewart
    Insert
    non-hairpin 18bp
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Promoter
    U6 promoter
    Available since
    Dec. 22, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    57 Articles
    13425-plasmid-map-sequence-id-360003

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  40. HRE-luciferase

    Plasmid
    #26731
    Purpose
    Luciferase reporter construct containing three hypoxia response elements (24-mers) from the Pgk-1 gene.
    Depositor
    Navdeep Chandel
    Publication
    Emerling et al Proc Natl Acad Sci U S A. 2008 Feb 19. 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Hypoxia Responsive Elements (3)
    Use
    Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Available since
    Nov. 10, 2010
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    90 Articles
    26731-plasmid-map-sequence-id-222823

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  43. pCXWB-EBNA1

    Plasmid
    #37624
    Purpose
    Non-replicating episomal expression of EBNA1, which enhances transfection efficiency and expression from episomal plasmids
    Depositor
    Shinya Yamanaka
    Publication
    Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EBNA-1 (EBNA-1 human herpesvirus 4)
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Dec. 3, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    72 Articles
    37624-plasmid-map-sequence-id-47356

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  44. pAAV-CAG-GFP

    Plasmid
    #37825
    Purpose
    AAV-mediated expression of GFP under the CAG promoter
    Depositor
    Edward Boyden
    Has service
    AAV1, AAV1 trial size, AAV2, AAV2 trial size, AAV5, AAV5 trial size, AAV6, AAV6 trial size, AAV8, AAV8 trial size, AAV9, AAV9 trial size, AAV9-X1.1, AAV11, AAV11 trial size, AAV CAP-B10, AAV CAP-B22, AAV MaCPNS1, AAV MaCPNS2, AAV PHP.eB, AAV Retrograde, and AAV Retrograde trial size
    Insert
    GFP
    Use
    AAV; Adeno-associated virus
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CAG
    Available since
    Aug. 8, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    169 Articles
    37825-plasmid-map-sequence-id-448204

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  47. Lentiviral-TOP-dGFP-reporter

    Plasmid
    #14715
    Purpose
    Beta-catenin reporter - 3rd generation self-inactivating lentiviral plasmid LEF-1/TCF-responsive promoter driven d2-eGFP (destabilized, half-life of 2h).
    Depositor
    Tannishtha Reya
    Publication
    Reya et al Nature. 2003 May 22. 423(6938):409-14.
    Insert
    LEF-1/TCF-responsive promoter (Ctnnb1 )
    Use
    Lentiviral
    Tags
    d2 eGFP
    Expression
    Mammalian
    Available since
    Aug. 28, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    11 Articles
    14715-plasmid-map-sequence-id-339924

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Showing: 201 - 250 of 177145 results