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Showing: 1651 - 1700 of 175573 results

  12. pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)

    Plasmid
    #47868
    Purpose
    This plasmid contains expression cassette for NmCas9 with N and C NLS and an HA tag, a cassette for expression of tracrRNA, a cassette for cloning crRNA under the control of U6 promoter.
    Depositor
    Erik Sontheimer, James Thomson
    Article
    Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.
    Inserts
    NmCas9
    U6pr-tracrRNA
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    EF1a and U6
    Available Since
    Sept. 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    47868-plasmid-map-sequence-id-68833

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  14. hprtSAGFP

    Plasmid
    #41594
    Purpose
    In vivo single-strand annealing substrate composed of two truncated GFP genes, separated by a drug selection marker, and flanked by mouse hprt targeting sequences
    Depositor
    Maria Jasin
    Article
    Stark et al Mol Cell Biol. 2004 Nov;24(21):9305-16.
    Insert
    SA-GFP reporter
    Use
    Mouse Targeting; Dsb repair reporter substrate
    Expression
    Mammalian
    Available Since
    Dec. 3, 2012
    Availability
    Academic Institutions and Nonprofits only
    41594-plasmid-map-sequence-id-228244

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  16. eIF2a 3

    Plasmid
    #21809
    Depositor
    David Ron
    Insert
    eIF2a (EIF2S1 Human)
    Expression
    Mammalian
    Mutation
    S52D
    Available Since
    Sept. 10, 2009
    Availability
    Academic Institutions and Nonprofits only
    21809-plasmid-map-sequence-id-358561

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  28. pET28a/Cas9-Cys

    Plasmid
    #53261
    Purpose
    Expresses N-terminal His tag fused human codon-optimized Cas9 nuclease having C-terminal Cysteine
    Depositor
    Hyongbum Kim
    Article
    Ramakrishna et al Genome Res. 2014 Apr 2.
    Insert
    Cas9-Cys
    Tags
    6X His tag, HA epitope, and NLS
    Expression
    Bacterial
    Mutation
    Additional Cysteine is added to the C-terminal re…
    Promoter
    T7 Promoter
    Available Since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only
    53261-plasmid-map-sequence-id-261354

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  29. pAAV-hSyn-DIO-mCherry

    Plasmid
    #50459
    Purpose
    Double floxed mCherry under the control of human synapsin promoter
    Depositor
    Bryan Roth
    Has Service
    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    mCherry
    Use
    AAV
    Tags
    N/A
    Promoter
    human Synapsin 1
    Available Since
    March 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    50459-plasmid-map-sequence-id-171068

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  30. GPD-roGFP

    Plasmid
    #49436
    Purpose
    Expresses thiol redox-sensitive ratiometric sensor roGFP in the mitochondrial intermembrane space of mammalian cells
    Depositor
    Paul Schumacker
    Article
    Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi
    Insert
    GPD-roGFP2
    Use
    Adenoviral
    Tags
    Glycerol phosphate dehydrogenase, 5' to roGF…
    Expression
    Mammalian
    Mutation
    C48S, Q80R, S147C, and Q204C in Clontech's G…
    Promoter
    CMV
    Available Since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    49436-plasmid-map-sequence-id-251833

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  45. pRSETB/mStayGold

    Plasmid
    #212017
    Purpose
    mStayGold has an n1 adaptor at its N-terminus and is useful for C-terminal tagging. This construct is used also for expression of mStayGold itself.
    Depositor
    Atsushi Miyawaki
    Article
    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s
    Type
    Empty backbone
    Tags
    6xHis, T7 tag, Xpress tag, mStayGold
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    212017-plasmid-map-sequence-id-419228

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  46. pCMV-T7-EGFP (BPK1098)

    Plasmid
    #133962
    Purpose
    CMV and T7 promoter plasmid for EGFP expression in mammalian cells or for in vitro transcription
    Depositor
    Benjamin Kleinstiver
    Insert
    EGFP
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available Since
    Feb. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    133962-plasmid-map-sequence-id-265771

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  49. pLenti-sgRNA

    Plasmid
    #71409
    Purpose
    There is no gene/insert. It is a backbone plasmid that others can use to insert sgRNAs of interest. The cloning site for this BsmBI
    Depositor
    Eric Lander, David Sabatini
    Type
    Empty backbone
    Use
    Lentiviral
    Available Since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71409-plasmid-map-sequence-id-322475

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Showing: 1651 - 1700 of 175573 results