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177,145 results

Showing: 1651 - 1700 of 177145 results

  4. pLVPT-rtTR-KRAB-2SM2

    Plasmid
    #11652
    Purpose
    Tet-regulated (Tet-off) lentiviral vector for transgene (mPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation
    Depositor
    Patrick Aebischer, Didier Trono
    Publication
    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:
    Insert
    mPGK, GFP, rtTR-KRAB-2SM2, Tet-off
    Use
    Lentiviral
    Expression
    Mammalian
    Available since
    Aug. 17, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    11652-plasmid-map-sequence-id-6998

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  6. pBOB-CAG-SARS-CoV2-Spike-HA

    Plasmid
    #141347
    Purpose
    3rd Generation lentiviral vector expressing the codon-optimized SARS-CoV2 Spike Glycoprotein ORF with a C-terminal HA tag generated by Junko Ogawa & Gerald M Pao
    Depositor
    Gerald Pao
    Publication
    Ogawa et al bioRxiv. 2020 Jul 22. doi: 10.1101/2020.
    Insert
    SARS-CoV2 Spike Glycoprotein (S SARS-CoV2 hCoV19_USA EPI_ISL_414366 (GISAID))
    Use
    Lentiviral
    Tags
    HA
    Mutation
    resynthesized with human codon optimization nucle…
    Promoter
    CAG
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    8 Articles
    141347-plasmid-map-sequence-id-296391

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  7. pCMV-T7-EGFP (BPK1098)

    Plasmid
    #133962
    Purpose
    CMV and T7 promoter plasmid for EGFP expression in mammalian cells or for in vitro transcription
    Depositor
    Benjamin Kleinstiver
    Insert
    EGFP
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available since
    Feb. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    133962-plasmid-map-sequence-id-265771

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  8. pLVX-UbC-rtTA-Ngn2:2A:Ascl1

    Plasmid
    #127289
    Purpose
    all-in-one inducible LV expression construct for Ngn2:2A:Ascl1 (aka UNA; for direct neuronal conversion)
    Depositor
    Fred Gage
    Publication
    Herdy et al Elife. 2019 May 17;8. pii: 41356. doi: 1
    Inserts
    Ngn2:2A:Ascl1 (ASCL1, NEUROG2 Human)
    Ngn2:2A:Ascl1 (ASCL1, NEUROG2 Human)
    Use
    Lentiviral
    Tags
    Ngn2 and Ascl1 linked via 2A
    Expression
    Mammalian
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    13 Articles
    127289-plasmid-map-sequence-id-308425

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  9. pAAV-hSyn-DIO-mCherry

    Plasmid
    #50459
    Purpose
    Double floxed mCherry under the control of human synapsin promoter
    Depositor
    Bryan Roth
    Has service
    AAV1, AAV2, AAV5, AAV8, AAV9, and AAV Retrograde
    Insert
    mCherry
    Use
    AAV
    Tags
    N/A
    Promoter
    human Synapsin 1
    Available since
    March 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    379 Articles
    50459-plasmid-map-sequence-id-171068

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  10. hprtSAGFP

    Plasmid
    #41594
    Purpose
    In vivo single-strand annealing substrate composed of two truncated GFP genes, separated by a drug selection marker, and flanked by mouse hprt targeting sequences
    Depositor
    Maria Jasin
    Publication
    Stark et al Mol Cell Biol. 2004 Nov;24(21):9305-16.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SA-GFP reporter
    Use
    Mouse Targeting; Dsb repair reporter substrate
    Expression
    Mammalian
    Available since
    Dec. 3, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    10 Articles
    41594-plasmid-map-sequence-id-228244

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  12. eIF2a 3

    Plasmid
    #21809
    Depositor
    David Ron
    Insert
    eIF2a (EIF2S1 Human)
    Expression
    Mammalian
    Mutation
    S52D
    Available since
    Sept. 10, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    21809-plasmid-map-sequence-id-358561

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  17. pDY1052 PASTEv3 pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

    Plasmid
    #179105
    Purpose
    pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT
    Depositor
    Omar Abudayyeh, Jonathan Gootenberg
    Publication
    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    179105-plasmid-map-sequence-id-421140

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  22. pEGFP-C1 F-tractin-EGFP

    Plasmid
    #58473
    Purpose
    Expresses a cytoplasmic actin filament reporter, the neuronal inositol 1,4,5-triphosphate 3-kinase A actin-binding domain known as F-tractin, on a CMV promoter
    Depositor
    Dyche Mullins
    Publication
    Belin et al Bioarchitecture. 2015 Aug 28:1-14.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    F-tractin (Itpka Rat)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    28 Articles
    58473-plasmid-map-sequence-id-244703

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  25. pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)

    Plasmid
    #47868
    Purpose
    This plasmid contains expression cassette for NmCas9 with N and C NLS and an HA tag, a cassette for expression of tracrRNA, a cassette for cloning crRNA under the control of U6 promoter.
    Depositor
    Erik Sontheimer, James Thomson
    Publication
    Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.
    Inserts
    NmCas9
    U6pr-tracrRNA
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    EF1a and U6
    Available since
    Sept. 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    47868-plasmid-map-sequence-id-68833

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  34. pLVX-Tet3G blasticidin

    Plasmid
    #128061
    Purpose
    Lentiviral vector encoding the Tet-On 3G transactivator protein, which allows for inducible gene expression. With Blasticidin resistance.
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available since
    Aug. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    128061-plasmid-map-sequence-id-255115

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  38. pLenti-sgRNA

    Plasmid
    #71409
    Purpose
    There is no gene/insert. It is a backbone plasmid that others can use to insert sgRNAs of interest. The cloning site for this BsmBI
    Depositor
    Eric Lander, David Sabatini
    Type
    Empty backbone
    Use
    Lentiviral
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    20 Articles
    71409-plasmid-map-sequence-id-322475

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  40. pET28a/Cas9-Cys

    Plasmid
    #53261
    Purpose
    Expresses N-terminal His tag fused human codon-optimized Cas9 nuclease having C-terminal Cysteine
    Depositor
    Hyongbum Kim
    Publication
    Ramakrishna et al Genome Res. 2014 Apr 2.
    Insert
    Cas9-Cys
    Tags
    6X His tag, HA epitope, and NLS
    Expression
    Bacterial
    Mutation
    Additional Cysteine is added to the C-terminal re…
    Promoter
    T7 Promoter
    Available since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    23 Articles
    53261-plasmid-map-sequence-id-261354

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  41. pLenti.PGK.LifeAct-GFP.W

    Plasmid
    #51010
    Purpose
    Lentivector that expresses LifeAct-GFP from internal human PGK promoter
    Depositor
    Rusty Lansford
    Insert
    LifeAct-GFP
    Use
    Lentiviral
    Tags
    LifeAct
    Expression
    Mammalian
    Promoter
    human PGK
    Available since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    26 Articles
    51010-plasmid-map-sequence-id-291815

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  42. GPD-roGFP

    Plasmid
    #49436
    Purpose
    Expresses thiol redox-sensitive ratiometric sensor roGFP in the mitochondrial intermembrane space of mammalian cells
    Depositor
    Paul Schumacker
    Publication
    Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi
    Insert
    GPD-roGFP2
    Use
    Adenoviral
    Tags
    Glycerol phosphate dehydrogenase, 5' to roGF…
    Expression
    Mammalian
    Mutation
    C48S, Q80R, S147C, and Q204C in Clontech's G…
    Promoter
    CMV
    Available since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    49436-plasmid-map-sequence-id-251833

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Showing: 1651 - 1700 of 177145 results