173,051 results

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mCherry

Plasmid

#176016

Purpose

Mammalian expression of mCherry. Parton lab clone DFQ

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

mCherry

Expression

Mammalian

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-PEmax-P2A-GFP

Plasmid

#180020

Purpose

Mammalian expression of SpCas9 PEmax prime editor with P2A-EGFP marker

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

PEmax-P2A-EGFP

Tags

P2A-EGFP, SV40 bpNLS, and c-Myc NLS

Expression

Mammalian

Mutation

Detailed in manuscript

Promoter

CMV

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) eGFP

Plasmid

#129020

Purpose

Expresses eGFP

Depositor

Jeremy Wilusz

Article

Xiao et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

Insert

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pETcon(-)

Plasmid

#41522

Depositor

Andrew Scharenberg

Type

Empty backbone

Tags

3x(G4S) Linker, G3S Linker, HA, Myc Tag, and Stuf…

Expression

Yeast

Promoter

GAL1

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pLIX403_RPS2_APOBEC_HA_P2A_mRuby_Capture1

Plasmid

#194703

Purpose

Inducible lentiviral expression, TRE-RPS2-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA (Ribo-STAMP, RPS2)

Depositor

Eugene Yeo

Article

Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

Insert

RPS2 (RPS2 Human)

Use

Lentiviral

Tags

APOBEC1-HA-P2A-mRuby

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pEF1a-hMLH1dn

Plasmid

#174824

Purpose

Mammalian expression of human MLH1dn (codon optimized)

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

human MLH1dn (codon optimized)

Expression

Mammalian

Mutation

deletion of residues 754-756

Promoter

EF1a

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-GCaMP6s

Plasmid

#40753

Purpose

Mammalian expression of ultrasensitive protein calcium sensor

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Insert

GCaMP6s

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pX330-U6-Chimeric_BB-CBh-hSpCas9

Plasmid

#42230

Purpose

A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Has Service

Cloning Grade DNA

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pBBR1MCS-2

Plasmid

#85168

Purpose

Mobilisable shuttle and expression vector. Replicates in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Cloned genes driven by derepressed lac promoter.

Depositor

Kenneth Peterson

Article

Kovach et al Gene. 1995 Dec 1;166(1):175-6.

Has Service

Cloning Grade DNA

Type

Empty backbone

Expression

Bacterial

Promoter

Constitutive (derepressed P-lac)

Available Since

Nov. 8, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pLX304 CMV HA-HaloTag-FRB-sTurboID-high affinity (C)

Plasmid

#153005

Purpose

Expresses high-affinity split-TurboID C-terminal fragment in mammalian cells

Depositor

Alice Ting

Article

Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

Insert

sTurboID-high affinity (C) (G79)

Use

Lentiviral

Tags

FRB, HA, and HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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SrtA-PDGFR Tm

Plasmid

#121168

Purpose

pMP71 vector expressing Tomato - P2A - flag - SrtA - PDGFR Tm

Depositor

Gabriel Victora

Article

Pasqual et al Nature. 2018 Jan 25;553(7689):496-500. d

Insert

SrtA-PDGFR

Use

Retroviral

Tags

Tomato and flag

Expression

Mammalian

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLARRYv2-EGFP

Plasmid

#233208

Purpose

Cloning expressed barcodes at the EcoRV site for preparing lentiviral libaries for single-cell lineage tracing experiments, with EGFP marker

Depositor

Alejo Rodriguez-Fraticelli

Article

Singh et al Cell Stem Cell. 2025 Feb 19:S1934-5909(2

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-TurboID-NES_pLX304

Plasmid

#202008

Purpose

expresses TurboID in the mammalian cytosol, lentiviral vector

Depositor

Alice Ting

Article

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

Flag-TurboID-NES

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pLX311 Luciferase

Plasmid

#117735

Purpose

Open reading frame vector encoding Luciferase

Depositor

William Hahn

Article

Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

Insert

firefly Luciferase

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pMT525

Plasmid

#131392

Purpose

L1RP retrotransposition reporter

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

L1RP GFP-AI

Expression

Mammalian

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ATR WT

Plasmid

#31611

Depositor

Aziz Sancar

Article

Jiang et al Mol Cell Biol. 2006 Jan . 26(1):39-49.

Insert

ATR (ATR Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-Fibrillarin

Plasmid

#26673

Depositor

Sui Huang

Article

Chen et al J Cell Biol. 2001 Apr 2. 153(1):169-76.

Insert

Fibrillarin (FBL Human)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only

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v5 Cas9-eVLP

Plasmid

#228468

Purpose

Expresses MMLVgag(C507V)–Cas9 for producing v5 Cas9-eVLPs

Depositor

David Liu

Article

Raguram et al Nat Biotechnol. 2024 Nov 13. doi: 10.103

Insert

MMLVgag(C507V)–Cas9

Tags

FLAG

Expression

Mammalian

Mutation

MMLVgag(C507V)

Promoter

CMV

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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p6XHis-T7(P266L)

Plasmid

#174866

Purpose

Expresses a T7 RNA Polymerase that produces long RNA transcripts in high yield and generates fewer abortive transcripts.

Depositor

Anna Pyle

Article

Chillon et al Methods Enzymol. 2015;558:3-37. doi: 10.

Insert

T7 RNA polymerase

Tags

6XHis-tag

Expression

Bacterial

Mutation

P266L

Promoter

lac promoter

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-Bsd-CMV>tTS/rtTA:T2A:{cymR}

Plasmid

#236251

Purpose

Blasticidin selected lentiviral vector with Tet-On regulatory proteins and the cumate repressor, for use with dual-inducible CymR-CuO system

Depositor

Genevieve Kendall

Article

Kent et al Sci Rep. 2024 Sep 5;14(1):20773. doi: 10

Inserts

tTS/rtTA
cymR

Use

Lentiviral

Expression

Mammalian

Mutation

rTS and rtTA_M2 linked by T2A

Promoter

CMV

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Puro-{CMV-CuO}>{MCS}:T2A:mCherry

Plasmid

#236249

Purpose

Puromycin selected lentiviral vector with MCS for cumate-inducible gene, for use with dual-inducible CymR-CuO system

Depositor

Genevieve Kendall

Article

Kent et al Sci Rep. 2024 Sep 5;14(1):20773. doi: 10

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-CuO

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pDZ2087

Plasmid

#92414

Purpose

Expresses His-tagged mutant (L56V/S135G/S219V) TEV protease in E. coli

Depositor

David Waugh

Article

Raran-Kurussi et al Methods Mol Biol. 2017;1586:221-230. doi

Insert

TEV protease

Tags

MBP (self cleaving)

Expression

Bacterial

Mutation

L56V/S135G/S219V

Promoter

tac

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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ubbr:GCaMP8m

Plasmid

#232485

Purpose

To make fish transgenic lines that have calcium sensor expression in the whole body of the fish

Depositor

Chie Satou

Article

Ruetten et al bioRxiv [Preprint]. 2025 Sep 20:2025.08.

Insert

jGCaMP8m

Use

Zebrafish expression

Promoter

ubbr

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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peYFP-C1-mTOR

Plasmid

#73384

Purpose

Expresses YFP tagged mTOR

Depositor

Jie Chen, Taekjip Ha

Article

Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

Insert

mTOR (MTOR Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pHiUGE TurboID-HA donor (all-in-one) ORF0

Plasmid

#200383

Purpose

Tagging endogenously expressed proteins with TurboID-HA, C-terminal

Depositor

Scott Soderling

Article

Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

Insert

TurboID (ref: PMID: 30125270, Addgene plasmid # 107169)

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pHiUGE TurboID-HA donor (all-in-one) ORF1

Plasmid

#200384

Purpose

Tagging endogenously expressed proteins with TurboID-HA, C-terminal

Depositor

Scott Soderling

Article

Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

Insert

TurboID (ref: PMID: 30125270, Addgene plasmid # 107169)

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHiUGE TurboID-HA donor (all-in-one) ORF2

Plasmid

#200385

Purpose

Tagging endogenously expressed proteins with TurboID-HA, C-terminal

Depositor

Scott Soderling

Article

Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

Insert

TurboID (ref: PMID: 30125270, Addgene plasmid # 107169)

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pTRKH3-ermGFP

Plasmid

#27169

Depositor

Michela Lizier

Article

Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

Insert

erythromycin ribosomal methylase promoter region fused with modified green fluorescent protein 5

Expression

Bacterial

Mutation

presence of an additional 183-bp region at N term…

Available Since

Feb. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pKD4

Plasmid

#45605

Depositor

Barry L. Wanner

Article

Datsenko et al Proc Natl Acad Sci U S A. 2000 Jun 6;97(

Insert

FRT-kan-FRT

Expression

Bacterial

Promoter

R6K

Available Since

June 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
BPK1520

Plasmid

#65777

Purpose

Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

SpCas9 gRNA backbone, without spacer sequence

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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FUGW

Plasmid

#14883

Purpose

3rd gen lentiviral plasmid with hUbC-driven EGFP; can be used for cDNA expresion

Depositor

David Baltimore

Article

Lois et al Science. 2002 Feb 1. 295(5556):868-72.

Insert

flap-Ub promoter-GFP-WRE

Use

Lentiviral

Expression

Mammalian

Available Since

May 1, 2007

Availability

Academic Institutions and Nonprofits only

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MSCV-IRES-Thy1.1 DEST

Plasmid

#17442

Purpose

Destination vector derived from MSCV-IRES-Thy1.1. Please note that this plasmid contains attB1 and attB2 sites for use in a BP reaction

Depositor

Anjana Rao

Article

Wu et al Cell. 2006 Jul 28. 126(2):375-87.

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-ABE8e-NRCH

Plasmid

#165416

Purpose

Expresses the base editor ABE8e-NRCH in mammalian cells

Depositor

David Liu

Insert

ABE8e-NRCH

Tags

BP-NLS

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Cbh_v5 AAV-CBE C-terminal

Plasmid

#137176

Purpose

AAV genome: expresses the C-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNA

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

v5 AAV-CBE C-terminal; U6-protospacer

Use

AAV

Promoter

Cbh

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLJM1-Halo-RNaseL

Plasmid

#250856

Purpose

lentiviral vector for expressing Halo-tagged RNase L

Depositor

Britt Glaunsinger

Insert

RNASEL (RNASEL Human)

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Promoter

CMV promoter

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pLTR-RD114A

Plasmid

#17576

Depositor

Jakob Reiser

Article

Zhang et al J Virol. 2004 Feb . 78(3):1219-29.

Insert

RD114 envelope glycoprotein

Use

Lentiviral

Expression

Mammalian

Available Since

April 14, 2008

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
phage UbiC G3BP1-GFP-GFP

Plasmid

#119950

Purpose

Lentiviral vector expressing G3BP1-GFP fusion protein

Depositor

Jeffrey Chao

Article

Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

G3BP1 (G3BP1 Human)

Use

Lentiviral

Tags

GFP

Expression

Bacterial and Mammalian

Promoter

UbiC

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDH-EF1

Plasmid

#72266

Purpose

Express gene of interest under EF-1 promoter

Depositor

Kazuhiro Oka

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 11, 2016

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNA3-OVA

Plasmid

#64599

Purpose

Expression of chicken ovalbumin as model antigen for study of MHC class I and class II presentation

Depositor

Sandra Diebold, Martin Zenke

Article

Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

Insert

Ovalbumin

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pHCMV_Bless

Plasmid

#207255

Purpose

Mammalian expression of Baboon Endogenous Retrovirus env protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

BaboonEndogenousRetrovirus_Rless_env

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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173,051 results