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169,512 results

Showing: 301 - 350 of 169512 results

  1. ADA

    Plasmid
    #39020
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ADA (ADA Human)
    Tags
    TEV-His6-Flag
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  5. pLV_Zika_NS1_Flag (Variant: W98G)

    Plasmid
    #79641
    Purpose
    Third generation Self-inactivating lentivector expressing NS1 (Variant W98G) from Zika virus
    Depositor
    Vaithi Arumugaswami
    Insert
    NS1
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Variant: W98G
    Promoter
    EF1
    Available Since
    Sept. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    79641-plasmid-map-sequence-id-152664

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  14. pAAV-nEF-Con/Foff 2.0-ChRmine-oScarlet

    Plasmid
    #137161
    Purpose
    Intersectional viral expression of ChRmine-p2a-oScarlet in cells expressing Cre AND NOT Flp
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Has Service
    AAV8
    Insert
    Con/Foff 2.0-ChRmine-p2a-oScarlet
    Use
    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt
    Mutation
    oScarlet E95D
    Promoter
    nEF
    Available Since
    June 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    137161-plasmid-map-sequence-id-272782

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  15. BTV

    Plasmid
    #84771
    Purpose
    bidirectional tetracycline-regulated viral reporter that measures the effects of 3' UTR sequences on mRNA and protein production
    Depositor
    David Erle
    Article
    Zhao et al Nat Biotechnol. 2014 Apr;32(4):387-91. d
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    Oct. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    84771-plasmid-map-sequence-id-162487

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  19. Unfused click editor construct; PCV2-EcKlenow fusion - pCMV-T7-PCV2-EcKlenow (JO1421)

    Plasmid
    #217804
    Purpose
    Unfused click editor construct expressing PCV2-EcKlenow, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-linker-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217804-plasmid-map-sequence-id-432897

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  22. lentiCRISPRv2-sgRNAs-del-CDK5RAP2-SVA-BFP

    Plasmid
    #202824
    Purpose
    Lentiviral vector modified to express two sgRNAs that delete the intronic SVA within the CDK5RAP2 gene with EF-1apha for Cas9 and BFP expression
    Depositor
    Matthew Anderson
    Article
    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:
    Insert
    two sgRNAs (each with a U6 promoter) to delete SVA within CDK5RAP2 gene
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    U6 - two
    Available Since
    July 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    202824-plasmid-map-sequence-id-399641

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  34. Control click editor - pCMV-T7-PCV2-deadCas9-EcKlenow (EMK500)

    Plasmid
    #208944
    Purpose
    A control CE1 construct with catalytically inactivated SpCas9, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-dSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    dSpCas9(D10A/H840A);EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    208944-plasmid-map-sequence-id-415672

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  40. ADH4

    Plasmid
    #38910
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ADH4 (ADH4 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  43. PICK1

    Plasmid
    #38803
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PICK1 (Pick1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  49. pDBB56

    Plasmid
    #248963
    Purpose
    Optogenetic expression of Shh driven by five GAL4/GAVPO binding sites in front of a synthetic YB-TATA minimal promoter in a PiggyBac integration plasmid.
    Depositor
    James Briscoe
    Article
    Benzinger et al Dev Cell. 2025 Aug 19:S1534-5807(25)0049
    Insert
    Shh-IRES2-H2B-mScarletI
    Expression
    Mammalian
    Promoter
    UASybTATA
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    248963-plasmid-map-sequence-id-499031

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Showing: 301 - 350 of 169512 results