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165,688 results

Showing: 1351 - 1400 of 165688 results

  5. T7-VEE-GFP

    Plasmid
    #58977
    Purpose
    expression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon
    Depositor
    Steven Dowdy
    Article
    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.
    Insert
    EGFP
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Expression
    Mammalian
    Promoter
    26S subgenomic promoter
    Available Since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    58977-plasmid-map-sequence-id-419580

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  8. pEVOL-pAzF

    Plasmid
    #31186
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAG
    Depositor
    Peter Schultz
    Article
    Chin et al J Am Chem Soc. 2002 Aug 7;124(31):9026-7
    Insert
    M.j. p-azidohenylalanine RS (2 copies +tRNA)
    Expression
    Bacterial
    Mutation
    Y32T, E107N, D158P, I159L, L162Q, D286R
    Available Since
    Dec. 9, 2011
    Availability
    Academic Institutions and Nonprofits only
    31186-plasmid-map-sequence-id-334781

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  9. pAAV-CAG-GFP

    Plasmid
    #37825
    Purpose
    AAV-mediated expression of GFP under the CAG promoter
    Depositor
    Edward Boyden
    Has Service
    AAV CAP-B10, AAV CAP-B22, AAV MaCPNS1, AAV MaCPNS2, AAV PHP.eB, AAV Retrograde, AAV Retrograde trial size, AAV1, AAV1 trial size, AAV11, AAV11 trial size, AAV2, AAV2 trial size, AAV5, AAV5 trial size, AAV6, AAV6 trial size, AAV8, AAV8 trial size, AAV9, AAV9 trial size, and AAV9-X1.1
    Insert
    GFP
    Use
    AAV; Adeno-associated virus
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CAG
    Available Since
    Aug. 8, 2012
    Availability
    Academic Institutions and Nonprofits only
    37825-plasmid-map-sequence-id-448204

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  11. pML104

    Plasmid
    #67638
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformation
    Depositor
    John Wyrick
    Article
    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1
    Inserts
    Cas9
    single guide RNA expression cassette
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    pSNR52 and pTDH3
    Available Since
    Aug. 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    67638-plasmid-map-sequence-id-322882

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  16. pcDNA5/FRT/TO-GAlphaQ-RLuc8

    Plasmid
    #140982
    Purpose
    Encodes a G alpha subunit (GNAQ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphaQ-RLuc8 (GNAQ Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140982-plasmid-map-sequence-id-300744

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  19. pCAP01

    Plasmid
    #59981
    Purpose
    The function of this material is to facilitate the capture and expression of bacterial gene clusters.
    Depositor
    Bradley Moore
    Article
    Yamanaka et al Proc Natl Acad Sci U S A. 2014 Feb 4;111
    Type
    Empty backbone
    Use
    Synthetic Biology; Transformation-associated reco…
    Tags
    no
    Expression
    Bacterial and Yeast
    Promoter
    no
    Available Since
    Nov. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    59981-plasmid-map-sequence-id-96247

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  20. pAAV-UBC-XRI-HA

    Plasmid
    #178056
    Purpose
    Expression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with HA tag, under UBC promoter.
    Depositor
    Edward Boyden
    Article
    Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    XRI-HA
    Use
    AAV
    Tags
    HA-MBP
    Expression
    Mammalian
    Promoter
    Human ubiquitin C (UBC) promoter
    Available Since
    Oct. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    178056-plasmid-map-sequence-id-349205

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  21. pDONR221-FURIN

    Plasmid
    #158442
    Purpose
    Gateway Cloning compatible entry vector for the human FURIN gene.
    Depositor
    Alejandro Chavez, Sho Iketani
    Insert
    FURIN (FURIN Human)
    Use
    Gateway entry vector
    Available Since
    Aug. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    158442-plasmid-map-sequence-id-308251

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  22. pCDH-EF1-Luc2-P2A-tdTomato

    Plasmid
    #72486
    Purpose
    Express Luc2 and tdTomato under EF1 promoter
    Depositor
    Kazuhiro Oka
    Insert
    Luc2-P2A-tdTomato
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1
    Available Since
    Jan. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    72486-plasmid-map-sequence-id-418875

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  29. pCRISPR-Cas3

    Plasmid
    #209427
    Purpose
    Plasmid for CASCADE-Cas3 based genome engineering of streptomycetes
    Depositor
    Tilmann Weber
    Article
    Whitford et al Nucleic Acids Res. 2025 Mar 20;53(6):gka
    Insert
    codon optimized minimal type I-C CASCADE-Cas3 from Pseudomonas aeruginosa
    Use
    CRISPR and Synthetic Biology
    Promoter
    PtipA; crRNA under control of PermE*
    Available Since
    March 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    209427-plasmid-map-sequence-id-416703

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  32. pCS2+MLS-HyPer7

    Plasmid
    #136470
    Purpose
    Mammalian expression of mitochondrial matrix targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging
    Depositor
    Vsevolod Belousov
    Article
    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.
    Insert
    HyPer7
    Tags
    Tandem mitochondrial targeting signal of cytochro…
    Expression
    Mammalian
    Promoter
    CMV, SP6
    Available Since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    136470-plasmid-map-sequence-id-271540

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  35. pSF3-ultraID

    Plasmid
    #172878
    Purpose
    expresses ultraID in mammalian cells with the tet-on or tet-off system
    Depositor
    Julien Béthune
    Article
    Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1
    Insert
    ultraID
    Use
    Luciferase and Retroviral; To be used in a tet-on…
    Tags
    myc
    Expression
    Mammalian
    Mutation
    fragment ([aa2-171]) of aquifex aeolicus BirA wit…
    Promoter
    TRE-cmv
    Available Since
    Aug. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    172878-plasmid-map-sequence-id-340526

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  37. Tau (P301L)-VC

    Plasmid
    #87633
    Purpose
    expresses hTau fused to the C-terminal part of Venus
    Depositor
    Tiago Outeiro
    Insert
    MAPT (MAPT Human)
    Tags
    N-terminal part of Venus
    Expression
    Mammalian
    Mutation
    Mutation P301L
    Promoter
    CMV
    Available Since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    87633-plasmid-map-sequence-id-196952

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  39. pET-pA-Hia5

    Plasmid
    #174372
    Purpose
    SEE DEPOSITOR COMMENTS BELOW. Plasmid for IPTG-inducible expression and purification of protein A-Hia5-6xHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)
    Depositor
    Aaron Straight
    Article
    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi
    Insert
    protein A - hia5
    Tags
    6xHis and Protein A
    Expression
    Bacterial
    Available Since
    Sept. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    174372-plasmid-map-sequence-id-341860

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  43. pMini-CMV-NLS-dead R-IscB (D60A, H269A)-NLS_T2A_mCherry_U6-ωRNA

    Plasmid
    #246430
    Purpose
    All-in-one plasmid. Expresses R-IscB in mammalian cells. Dead mutations included.
    Depositor
    Ailong Ke
    Article
    Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.
    Inserts
    O.gue IscB with dead mutations (D60A, H269A) and delta-TID
    mCherry
    ωRNA
    Tags
    SV40 NLS, Thosea asigna virus 2A peptide, and nuc…
    Expression
    Mammalian
    Mutation
    changed Aspartic Acid 60 to Alanine, changed Hist…
    Promoter
    CMV and U6
    Available Since
    Oct. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    246430-plasmid-map-sequence-id-490873

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  45. L4440

    Plasmid
    #1654
    Depositor
    Andrew Fire
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    RNAi
    Tags
    T7p, T7p, lacZN, OriF1>>, OriF1<<
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1654-plasmid-map-sequence-id-422970

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Showing: 1351 - 1400 of 165688 results