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177,145 results

Showing: 151 - 200 of 177145 results

  2. CRISPRoff-v2.1

    Plasmid
    #167981
    Purpose
    Expresses CRISPRoff-v2.1 (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-BFP-KRAB) downstream of the CAG promoter
    Depositor
    Luke Gilbert
    Publication
    Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB)
    Tags
    2xNLS, DNMT3A-DNMT3L, HA, KRAB, and tagBFP
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    May 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    20 Articles
    167981-plasmid-map-sequence-id-330241

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  3. mCherry2-N1

    Plasmid
    #54517
    Purpose
    Localization: N1 Cloning Vector, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mCherry2
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    47 Articles
    54517-plasmid-map-sequence-id-352457

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  6. pMitoTimer

    Plasmid
    #52659
    Purpose
    Expresses mitochondrial targeted DsRed1-E5 as reporter for mitochondrial content, structure, oxidative stress and damage in vitro and in vivo
    Depositor
    Zhen Yan
    Publication
    Laker et al J Biol Chem. 2014 Apr 25;289(17):12005-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DsRed1-E5
    Use
    Dsred
    Tags
    human cytochrome c oxidase subunit VIII mitochond…
    Promoter
    CMV
    Available since
    April 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    44 Articles
    52659-plasmid-map-sequence-id-430007

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  7. pGL3-NFAT luciferase

    Plasmid
    #17870
    Purpose
    3x NFAT binding sequence upstream of a luciferase reporter; can be used to assay signal transduction through Ca2+, calcineurin and NFAT in any tissue
    Depositor
    Jerry Crabtree
    Publication
    Clipstone et al Nature. 1992 Jun 25. 357(6380):695-7.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3x NFAT binding sequence (IL2 )
    Use
    Luciferase
    Tags
    IL2 promoter and Luciferase
    Expression
    Mammalian
    Promoter
    IL-2 promoter
    Available since
    April 29, 2008
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    41 Articles
    17870-plasmid-map-sequence-id-520532

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  16. pgRNA-humanized

    Plasmid
    #44248
    Purpose
    Expression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdown
    Depositor
    Stanley Qi
    Publication
    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1
    Insert
    guide RNA
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    mouse U6
    Available since
    April 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    57 Articles
    44248-plasmid-map-sequence-id-190219

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  18. pcDNA3-YFP

    Plasmid
    #13033
    Purpose
    Mammalian expression vector for cloning your gene fused to YFP
    Depositor
    Doug Golenbock
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Yellow fluorescent protein
    Expression
    Mammalian
    Available since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    33 Articles
    13033-plasmid-map-sequence-id-210331

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  20. pmRFP-LC3

    Plasmid
    #21075
    Purpose
    Mammalian expression of rat LC3 fused to mRFP
    Depositor
    Tamotsu Yoshimori
    Publication
    Kimura et al Autophagy. 2007;3(5):452-60.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    Tags
    mRFP
    Expression
    Mammalian
    Mutation
    First ATG is not included.
    Available since
    May 28, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    95 Articles
    21075-plasmid-map-sequence-id-364494

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  22. pCMV delta R8.2

    Plasmid
    #12263
    Purpose
    First-generation packaging plasmid. This plasmid contains HIV accessory genes. Please reach out to your biosafety officer for guidance.
    Depositor
    Didier Trono
    Insert
    HIV-1 GAG/POL, Tat and Rev
    Use
    Lentiviral; Packaging
    Expression
    Mammalian
    Available since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    238 Articles
    12263-plasmid-map-sequence-id-394974

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  24. L4440

    Plasmid
    #1654
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    RNAi
    Tags
    T7p, T7p, lacZN, OriF1>>, OriF1<<
    Expression
    Worm
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    93 Articles
    1654-plasmid-map-sequence-id-422970

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  25. pC0043-PspCas13b crRNA backbone

    Plasmid
    #103854
    Purpose
    For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac
    Depositor
    Feng Zhang
    Publication
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    U6-BbsI-BbsI-PspCas13b DR-polyT
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Nov. 28, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    42 Articles
    103854-plasmid-map-sequence-id-350782

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  26. pAAV2/1

    Plasmid
    #112862
    Purpose
    AAV packaging plasmid expressing Rep/Cap genes
    Depositor
    James M. Wilson
    Insert
    Rep2/Cap1
    Use
    AAV
    Promoter
    Truncated P5
    Available since
    Aug. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    46 Articles
    112862-plasmid-map-sequence-id-421935

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  27. pCMV-PEmax

    Plasmid
    #174820
    Purpose
    Mammalian expression of SpCas9 PEmax prime editor
    Depositor
    David Liu
    Publication
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PEmax
    Tags
    SV40 bpNLS and c-Myc NLS
    Expression
    Mammalian
    Mutation
    Detailed in manuscript
    Promoter
    CMV
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    26 Articles
    174820-plasmid-map-sequence-id-406249

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  28. MLM3613

    Plasmid
    #42251
    Purpose
    Expresses Cas9 nuclease (Streptococcus pyogenes) from CMV and T7 promoters
    Depositor
    Keith Joung
    Publication
    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Streptococcus pyogenes Cas9
    Use
    CRISPR; Zebrafish expression
    Promoter
    CMV
    Available since
    Jan. 31, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    114 Articles
    42251-plasmid-map-sequence-id-59208

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  29. pcDNA3-CFP

    Plasmid
    #13030
    Purpose
    Mammalian expression vector for cloning your gene fused to CFP
    Depositor
    Doug Golenbock
    Type
    Empty backbone
    Expression
    Mammalian
    Available since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    18 Articles
    13030-plasmid-map-sequence-id-212096

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  32. pRL-SV40P

    Plasmid
    #27163
    Purpose
    Renilla SV40 promoter (no enhancer) reporter gene for normalization of luciferase experiments
    Depositor
    Ron Prywes
    Publication
    Chen et al Mol Cell Biol. 1999 Jul . 19(7):4695-702
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SV40 promoter
    Use
    Luciferase
    Tags
    Renilla Luciferase
    Expression
    Mammalian
    Available since
    Feb. 25, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    83 Articles
    27163-plasmid-map-sequence-id-419582

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  38. pBABE GFP

    Plasmid
    #10668
    Purpose
    Empty backbone for retroviral gene expression. Select recipient cells using GFP.
    Depositor
    William Hahn
    Type
    Empty backbone
    Use
    Retroviral
    Expression
    Mammalian
    Available since
    Oct. 11, 2005
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    27 Articles
    10668-plasmid-map-sequence-id-106108

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  40. CD63-pEGFP C2

    Plasmid
    #62964
    Purpose
    full length human CD63 cloned into pEGFP C2
    Depositor
    Paul Luzio
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CD63 molecule (CD63 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    CD63 has 1 silent mutation compared to the coding…
    Promoter
    CMV
    Available since
    May 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    66 Articles
    62964-plasmid-map-sequence-id-207850

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  42. pCW-Cas9-Blast

    Plasmid
    #83481
    Purpose
    Lentiviral vector for mammalian expression of doxycycline-inducible Cas9 and constitutive expression of Blasticidin S deaminase -T2A -rtTA
    Depositor
    Mohan Babu
    Inserts
    SpCas9
    Blasticidin S deaminase -T2A - reverse tetracycline-controlled transactivator
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    Replaced puromycin N-acetyltransferase on the ori…
    Promoter
    Tight Tre and human phosphoglycerate kinase 1 pro…
    Available since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    34 Articles
    83481-plasmid-map-sequence-id-335118

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  46. pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro

    Plasmid
    #42333
    Purpose
    This plasmid separately encodes a human codon-optimized SpCas9 nickase, a tracrRNA and customizable crRNA.
    Depositor
    Feng Zhang
    Publication
    Cong et al Science. 2013 Jan 3.
    Insert
    humanized S. pyogenes Cas9 (D10A) nickase
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D10A nickase-converting mutation
    Available since
    March 7, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    42333-plasmid-map-sequence-id-59273

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  47. pcDNA3-mRuby2

    Plasmid
    #40260
    Purpose
    RFP with robust performance in protein fusions and useful as FRET acceptor for Clover in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap
    Depositor
    Michael Lin
    Publication
    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mRuby2
    Tags
    His6 and Xpress
    Expression
    Mammalian
    Promoter
    CMV IE
    Available since
    Sept. 20, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    47 Articles
    40260-plasmid-map-sequence-id-243381

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Showing: 151 - 200 of 177145 results