Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

177,145 results

Showing: 101 - 150 of 177145 results

  9. pBOB-EF1-FastFUCCI-Puro

    Plasmid
    #86849
    Purpose
    3rd generation lentiviral vector encoding FUCCI reporters for cell cycle analysis and puro for selection
    Depositor
    Kevin Brindle, Duncan Jodrell
    Publication
    Koh et al J Cell Sci. 2016 Nov 25. pii: jcs.195164
    Inserts
    mKO2-hCDT1(30-120)
    mAG-hGEM(1-110)
    pac (puromycin N-acetyl-transferase)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1 and PGK
    Available since
    Nov. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    46 Articles
    86849-plasmid-map-sequence-id-237968

    Enlarge

  10. lenti MS2-P65-HSF1_Hygro

    Plasmid
    #61426
    Purpose
    lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2
    Depositor
    Feng Zhang
    Publication
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Insert
    MS2-P65-HSF1_2A_Hygro (HSF1 Synthetic, Human)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    N55K in MS2
    Promoter
    EF1A
    Available since
    Dec. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    142 Articles
    61426-plasmid-map-sequence-id-140565

    Enlarge

  13. Lamp1-RFP

    Plasmid
    #1817
    Purpose
    Mammalian expression of Lamp1 fused to RFP. Used for localization to the lysosome.
    Depositor
    Walther Mothes
    Publication
    Sherer et al Traffic 2003 Nov;4(11):785-801.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    lysosome associated membrane protein 1 (Lamp1 Rat)
    Tags
    RFP
    Expression
    Mammalian
    Mutation
    D50E (not important for function of plasmid)
    Available since
    Sept. 26, 2005
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    98 Articles
    1817-plasmid-map-sequence-id-350820

    Enlarge

  19. lentiCRISPR v2-Blast

    Plasmid
    #83480
    Purpose
    Mammalian expression of Cas9 and sgRNA scaffold
    Depositor
    Mohan Babu
    Insert
    Blasticidin S deaminase
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    Replaced puromycin N-acetyltransferase on the ori…
    Promoter
    EF-1α
    Available since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    146 Articles
    83480-plasmid-map-sequence-id-423158

    Enlarge

  21. pCas9_GFP

    Plasmid
    #44719
    Purpose
    Co-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells
    Depositor
    Kiran Musunuru
    Publication
    Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    April 24, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    182 Articles
    44719-plasmid-map-sequence-id-472945

    Enlarge

  27. pBiFC-VC155

    Plasmid
    #22011
    Purpose
    Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays
    Depositor
    Chang-Deng Hu
    Publication
    Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    VC155
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Changed Alanine 206 to Lysine
    Available since
    Sept. 28, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    93 Articles
    22011-plasmid-map-sequence-id-354492

    Enlarge

  30. VP12

    Plasmid
    #72247
    Purpose
    Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A and Q926A
    Promoter
    CMV
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    29 Articles
    72247-plasmid-map-sequence-id-485302

    Enlarge

  32. pAG/MNase

    Plasmid
    #123461
    Purpose
    Expresses Protein A and Protein G IgG binding domains fused to micrococcal nuclease for purposes of CUT&RUN. Includes (his)6 tag for purification and HA-tag for secondary immunoprecipitation.
    Depositor
    Steven Henikoff
    Publication
    Meers et al Elife. 2019 Jun 24;8. pii: 46314. doi: 1
    Insert
    Protein A-Protein G-Micrococcal Nuclease
    Tags
    6xHIS and HA
    Expression
    Bacterial
    Promoter
    Lac
    Available since
    March 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    36 Articles
    123461-plasmid-map-sequence-id-417805

    Enlarge

  34. lentiMPH v2

    Plasmid
    #89308
    Purpose
    lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE). This version has a higher virus titer.
    Depositor
    Feng Zhang
    Publication
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Insert
    MS2-P65-HSF1_2A_Hygro (RELA Synthetic, Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    N55K in MS2
    Promoter
    EF1a
    Available since
    March 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    84 Articles
    89308-plasmid-map-sequence-id-425605

    Enlarge

  35. AAVS1 T2 CRIPR in pX330

    Plasmid
    #72833
    Purpose
    CRISPR/Cas to target the AAVS1 locus in human cells
    Depositor
    Masato Kanemaki
    Publication
    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9 and gRNA for targeting the AAVS1 locus in human cells
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6 promoter
    Available since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    40 Articles
    72833-plasmid-map-sequence-id-373023

    Enlarge

  37. pWPI

    Plasmid
    #12254
    Purpose
    2nd gen bicistronic lentiviral vector allows for simultaneous expression of a transgene and EGFP marker to facilitate tracking of transduced cells.
    Depositor
    Didier Trono
    Type
    Empty backbone
    Use
    Cre/Lox and Lentiviral
    Tags
    EMCV IRES-EGFP cassette
    Expression
    Mammalian
    Available since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    185 Articles
    12254-plasmid-map-sequence-id-485802

    Enlarge

  38. mRFP-Rab5

    Plasmid
    #14437
    Purpose
    Mammalian expression of Rab5 fused to mRFP. Used for localization to the early endosome, and other early endosomal vacuoles.
    Depositor
    Ari Helenius
    Publication
    Vonderheit et al PLoS Biol. 2005 Jul . 3(7):e233.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Rab 5 (RAB5A Human)
    Tags
    mRFP
    Expression
    Mammalian
    Available since
    March 23, 2007
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    82 Articles
    14437-plasmid-map-sequence-id-263462

    Enlarge

  40. LentiCRISPRv2-mCherry

    Plasmid
    #99154
    Purpose
    Lentiviral vector encoding sgRNA cloning site + hSpCAS9-P2A-mCherry.
    Depositor
    Agata Smogorzewska
    Inserts
    Cas9
    mCherry
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    EFS-NS
    Available since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58 Articles
    99154-plasmid-map-sequence-id-352449

    Enlarge

  44. SBE4-Luc

    Plasmid
    #16495
    Purpose
    Luciferase reporter containing four copies of the Smad binding site
    Depositor
    Bert Vogelstein
    Publication
    Zawel et al Mol Cell. 1998 Mar . 1(4):611-7.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Smad binding element
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mammalian
    Available since
    April 7, 2008
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    82 Articles
    16495-plasmid-map-sequence-id-333563

    Enlarge

  45. lentiSAMv2

    Plasmid
    #75112
    Purpose
    lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Publication
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available since
    May 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    41 Articles
    75112-plasmid-map-sequence-id-305764

    Enlarge

  46. pcDNA3-mRFP

    Plasmid
    #13032
    Purpose
    Mammalian expression vector for cloning your gene fused to mRFP
    Depositor
    Doug Golenbock
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Monomer Red Fluorescent Protein
    Expression
    Mammalian
    Available since
    Nov. 7, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    48 Articles
    13032-plasmid-map-sequence-id-211651

    Enlarge

  49. psPAX2-D64V

    Plasmid
    #63586
    Purpose
    psPAX2-D64V is derived from psPAX2 plasmid. It is a packaging plasmid for making Integrase deficient lentiviral vectors since has a point mutation in the integrase gene.
    Depositor
    David Rawlings, Andrew Scharenberg
    Publication
    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi
    Insert
    Packaging plasmid for integrase deficient lentiviral vectors
    Use
    Integrase deficient lentiviral packaging vector
    Available since
    April 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    19 Articles
    63586-plasmid-map-sequence-id-464218

    Enlarge

Showing: 101 - 150 of 177145 results