pX330-U6-Chimeric_BB-CBh-hSpCas9 Citations (1682)

Originally described in: Multiplex Genome Engineering Using CRISPR/Cas Systems.
Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F Science. 2013 Jan 3. PubMed Journal

---

Articles Citing pX330-U6-Chimeric_BB-CBh-hSpCas9

Articles
AP-1/IRF-3 Targeted Anti-Inflammatory Activity of Andrographolide Isolated from Andrographis paniculata. Shen T, Yang WS, Yi YS, Sung GH, Rhee MH, Poo H, Kim MY, Kim KW, Kim JH, Cho JY. Evid Based Complement Alternat Med. 2013;2013:210736. doi: 10.1155/2013/210736. Epub 2013 Jun 6. PubMed
CRISPR/Cas9 systems targeting beta-globin and CCR5 genes have substantial off-target activity. Cradick TJ, Fine EJ, Antico CJ, Bao G. Nucleic Acids Res. 2013 Nov;41(20):9584-92. doi: 10.1093/nar/gkt714. Epub 2013 Aug 11. PubMed
CRISPR/Cas-induced double-strand breaks boost the frequency of gene replacements for humanizing the mouse Cnr2 gene. Gennequin B, Otte DM, Zimmer A. Biochem Biophys Res Commun. 2013 Nov 29;441(4):815-9. doi: 10.1016/j.bbrc.2013.10.138. Epub 2013 Nov 6. PubMed
Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA. Mashiko D, Fujihara Y, Satouh Y, Miyata H, Isotani A, Ikawa M. Sci Rep. 2013 Nov 27;3:3355. doi: 10.1038/srep03355. PubMed Associated Plasmids pCAG-EGxxFP pCAG-EGxxFP-Cetn1 pX330-Cetn1/1
Mutagenesis and homologous recombination in Drosophila cell lines using CRISPR/Cas9. Bassett AR, Tibbit C, Ponting CP, Liu JL. Biol Open. 2013 Dec 10. pii: bio.20137120v1. doi: 10.1242/bio.20137120. PubMed Associated Plasmids pAc-sgRNA-Cas9 pAc-y1sgRNA-Cas9
Feasibility for a large scale mouse mutagenesis by injecting CRISPR/Cas plasmid into zygotes. Mashiko D, Young SA, Muto M, Kato H, Nozawa K, Ogawa M, Noda T, Kim YJ, Satouh Y, Fujihara Y, Ikawa M. Dev Growth Differ. 2014 Jan;56(1):122-9. doi: 10.1111/dgd.12113. Epub 2013 Dec 26. PubMed
A synthetic lethal screen identifies FAT1 as an antagonist of caspase-8 in extrinsic apoptosis. Kranz D, Boutros M. EMBO J. 2014 Feb 3;33(3):181-97. doi: 10.1002/embj.201385686. Epub 2014 Jan 17. PubMed
IgH class switching exploits a general property of two DNA breaks to be joined in cis over long chromosomal distances. Gostissa M, Schwer B, Chang A, Dong J, Meyers RM, Marecki GT, Choi VW, Chiarle R, Zarrin AA, Alt FW. Proc Natl Acad Sci U S A. 2014 Feb 18;111(7):2644-9. doi: 10.1073/pnas.1324176111. Epub 2014 Feb 3. PubMed
EFCAB7 and IQCE regulate hedgehog signaling by tethering the EVC-EVC2 complex to the base of primary cilia. Pusapati GV, Hughes CE, Dorn KV, Zhang D, Sugianto P, Aravind L, Rohatgi R. Dev Cell. 2014 Mar 10;28(5):483-96. doi: 10.1016/j.devcel.2014.01.021. Epub 2014 Feb 27. PubMed
Quantifying genome-editing outcomes at endogenous loci with SMRT sequencing. Hendel A, Kildebeck EJ, Fine EJ, Clark JT, Punjya N, Sebastiano V, Bao G, Porteus MH. Cell Rep. 2014 Apr 10;7(1):293-305. doi: 10.1016/j.celrep.2014.02.040. Epub 2014 Mar 27. PubMed
Targeted genome editing of sweet orange using Cas9/sgRNA. Jia H, Wang N. PLoS One. 2014 Apr 7;9(4):e93806. doi: 10.1371/journal.pone.0093806. eCollection 2014. PubMed
CRISPR/Cas9 allows efficient and complete knock-in of a destabilization domain-tagged essential protein in a human cell line, allowing rapid knockdown of protein function. Park A, Won ST, Pentecost M, Bartkowski W, Lee B. PLoS One. 2014 Apr 17;9(4):e95101. doi: 10.1371/journal.pone.0095101. eCollection 2014. PubMed Associated Plasmids PuroR-P2A-DD-TCOF1-donor BlastR-P2A-DD-TCOF1-donor
Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells. Wu X, Scott DA, Kriz AJ, Chiu AC, Hsu PD, Dadon DB, Cheng AW, Trevino AE, Konermann S, Chen S, Jaenisch R, Zhang F, Sharp PA. Nat Biotechnol. 2014 Jul;32(7):670-6. doi: 10.1038/nbt.2889. Epub 2014 Apr 20. PubMed
Targeted genomic rearrangements using CRISPR/Cas technology. Choi PS, Meyerson M. Nat Commun. 2014 Apr 24;5:3728. doi: 10.1038/ncomms4728. PubMed
An RNF168 fragment defective for focal accumulation at DNA damage is proficient for inhibition of homologous recombination in BRCA1 deficient cells. Munoz MC, Yanez DA, Stark JM. Nucleic Acids Res. 2014 Jul;42(12):7720-33. doi: 10.1093/nar/gku421. Epub 2014 May 14. PubMed
Simple generation of albino C57BL/6J mice with G291T mutation in the tyrosinase gene by the CRISPR/Cas9 system. Mizuno S, Dinh TT, Kato K, Mizuno-Iijima S, Tanimoto Y, Daitoku Y, Hoshino Y, Ikawa M, Takahashi S, Sugiyama F, Yagami K. Mamm Genome. 2014 Aug;25(7-8):327-34. doi: 10.1007/s00335-014-9524-0. Epub 2014 May 31. PubMed
LKB1 loss at transcriptional level promotes tumor malignancy and poor patient outcomes in colorectal cancer. He TY, Tsai LH, Huang CC, Chou MC, Lee H. Ann Surg Oncol. 2014 Dec;21 Suppl 4:S703-10. doi: 10.1245/s10434-014-3824-1. Epub 2014 May 31. PubMed
Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system. Ghorbal M, Gorman M, Macpherson CR, Martins RM, Scherf A, Lopez-Rubio JJ. Nat Biotechnol. 2014 Aug;32(8):819-21. doi: 10.1038/nbt.2925. Epub 2014 Jun 1. PubMed
Characterization of genomic deletion efficiency mediated by clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 nuclease system in mammalian cells. Canver MC, Bauer DE, Dass A, Yien YY, Chung J, Masuda T, Maeda T, Paw BH, Orkin SH. J Biol Chem. 2014 Aug 1;289(31):21312-24. doi: 10.1074/jbc.M114.564625. Epub 2014 Jun 6. PubMed
An iCRISPR Platform for Rapid, Multiplexable, and Inducible Genome Editing in Human Pluripotent Stem Cells. Gonzalez F, Zhu Z, Shi ZD, Lelli K, Verma N, Li QV, Huangfu D. Cell Stem Cell. 2014 Jun 11. pii: S1934-5909(14)00205-7. doi: 10.1016/j.stem.2014.05.018. PubMed Associated Plasmids Puro-Cas9 donor piCRg Entry AAVS1-TALEN-L AAVS1-TALEN-R
Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system. Sakuma T, Nishikawa A, Kume S, Chayama K, Yamamoto T. Sci Rep. 2014 Jun 23;4:5400. doi: 10.1038/srep05400. PubMed Associated Plasmids pX330A-1x2 pX330A-1x3 pX330A-1x4 pX330A-1x5 pX330A-1x6 More ...
Efficient editing of malaria parasite genome using the CRISPR/Cas9 system. Zhang C, Xiao B, Jiang Y, Zhao Y, Li Z, Gao H, Ling Y, Wei J, Li S, Lu M, Su XZ, Cui H, Yuan J. MBio. 2014 Jul 1;5(4):e01414-14. doi: 10.1128/mBio.01414-14. PubMed
Generating genetically modified mice using CRISPR/Cas-mediated genome engineering. Yang H, Wang H, Jaenisch R. Nat Protoc. 2014 Aug;9(8):1956-68. doi: 10.1038/nprot.2014.134. Epub 2014 Jul 24. PubMed
DNAVaxDB: the first web-based DNA vaccine database and its data analysis. Racz R, Li X, Patel M, Xiang Z, He Y. BMC Bioinformatics. 2014;15 Suppl 4:S2. doi: 10.1186/1471-2105-15-S4-S2. Epub 2014 Mar 19. PubMed
RIPK1 maintains epithelial homeostasis by inhibiting apoptosis and necroptosis. Dannappel M, Vlantis K, Kumari S, Polykratis A, Kim C, Wachsmuth L, Eftychi C, Lin J, Corona T, Hermance N, Zelic M, Kirsch P, Basic M, Bleich A, Kelliher M, Pasparakis M. Nature. 2014 Sep 4;513(7516):90-4. doi: 10.1038/nature13608. Epub 2014 Aug 17. PubMed
Understanding functional miRNA-target interactions in vivo by site-specific genome engineering. Bassett AR, Azzam G, Wheatley L, Tibbit C, Rajakumar T, McGowan S, Stanger N, Ewels PA, Taylor S, Ponting CP, Liu JL, Sauka-Spengler T, Fulga TA. Nat Commun. 2014 Aug 19;5:4640. doi: 10.1038/ncomms5640. PubMed
CTCF controls HOXA cluster silencing and mediates PRC2-repressive higher-order chromatin structure in NT2/D1 cells. Xu M, Zhao GN, Lv X, Liu G, Wang LY, Hao DL, Wang J, Liu DP, Liang CC. Mol Cell Biol. 2014 Oct;34(20):3867-79. doi: 10.1128/MCB.00567-14. Epub 2014 Aug 18. PubMed
CRISPR/Cas9-mediated gene knock-down in post-mitotic neurons. Straub C, Granger AJ, Saulnier JL, Sabatini BL. PLoS One. 2014 Aug 20;9(8):e105584. doi: 10.1371/journal.pone.0105584. eCollection 2014. PubMed
Saturation editing of genomic regions by multiplex homology-directed repair. Findlay GM, Boyle EA, Hause RJ, Klein JC, Shendure J. Nature. 2014 Sep 4;513(7516):120-3. doi: 10.1038/nature13695. PubMed
Heat-stable molecule derived from Streptococcus cristatus induces APOBEC3 expression and inhibits HIV-1 replication. Wang Z, Luo Y, Shao Q, Kinlock BL, Wang C, Hildreth JE, Xie H, Liu B. PLoS One. 2014 Aug 28;9(8):e106078. doi: 10.1371/journal.pone.0106078. eCollection 2014. PubMed
Efficient generation of genetically distinct pigs in a single pregnancy using multiplexed single-guide RNA and carbohydrate selection. Li P, Estrada JL, Burlak C, Montgomery J, Butler JR, Santos RM, Wang ZY, Paris LL, Blankenship RL, Downey SM, Tector M, Tector AJ. Xenotransplantation. 2015 Jan-Feb;22(1):20-31. doi: 10.1111/xen.12131. Epub 2014 Sep 2. PubMed
Improving the specificity and efficacy of CRISPR/CAS9 and gRNA through target specific DNA reporter. Zhang JH, Pandey M, Kahler JF, Loshakov A, Harris B, Dagur PK, Mo Y, Simonds WF. J Biotechnol. 2014 Sep 2. pii: S0168-1656(14)00838-4. doi: 10.1016/j.jbiotec.2014.08.033. PubMed Associated Plasmids psAAVS1-T2
Single-step generation of rabbits carrying a targeted allele of the tyrosinase gene using CRISPR/Cas9. Honda A, Hirose M, Sankai T, Yasmin L, Yuzawa K, Honsho K, Izu H, Iguchi A, Ikawa M, Ogura A. Exp Anim. 2015;64(1):31-7. doi: 10.1538/expanim.14-0034. Epub 2014 Sep 8. PubMed
CRISPR/Cas9 mediated generation of stable chondrocyte cell lines with targeted gene knockouts; analysis of an aggrecan knockout cell line. Yang M, Zhang L, Stevens J, Gibson G. Bone. 2014 Dec;69:118-25. doi: 10.1016/j.bone.2014.09.005. Epub 2014 Sep 26. PubMed
Mouse Genome Editing Using the CRISPR/Cas System. Harms DW, Quadros RM, Seruggia D, Ohtsuka M, Takahashi G, Montoliu L, Gurumurthy CB. Curr Protoc Hum Genet. 2014 Oct 1;83:15.7.1-15.7.27. doi: 10.1002/0471142905.hg1507s83. PubMed Associated Plasmids pBGK
Efficient gene targeting in golden Syrian hamsters by the CRISPR/Cas9 system. Fan Z, Li W, Lee SR, Meng Q, Shi B, Bunch TD, White KL, Kong IK, Wang Z. PLoS One. 2014 Oct 9;9(10):e109755. doi: 10.1371/journal.pone.0109755. eCollection 2014. PubMed
Easy quantitative assessment of genome editing by sequence trace decomposition. Brinkman EK, Chen T, Amendola M, van Steensel B. Nucleic Acids Res. 2014 Dec 16;42(22):e168. doi: 10.1093/nar/gku936. Epub 2014 Oct 9. PubMed
Control of cell identity genes occurs in insulated neighborhoods in mammalian chromosomes. Dowen JM, Fan ZP, Hnisz D, Ren G, Abraham BJ, Zhang LN, Weintraub AS, Schuijers J, Lee TI, Zhao K, Young RA. Cell. 2014 Oct 9;159(2):374-87. doi: 10.1016/j.cell.2014.09.030. PubMed
In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system. Maddalo D, Manchado E, Concepcion CP, Bonetti C, Vidigal JA, Han YC, Ogrodowski P, Crippa A, Rekhtman N, de Stanchina E, Lowe SW, Ventura A. Nature. 2014 Dec 18;516(7531):423-7. doi: 10.1038/nature13902. Epub 2014 Oct 22. PubMed Associated Plasmids Adeno EA Adeno Cas9 pX333
Creating class I MHC-null pigs using guide RNA and the Cas9 endonuclease. Reyes LM, Estrada JL, Wang ZY, Blosser RJ, Smith RF, Sidner RA, Paris LL, Blankenship RL, Ray CN, Miner AC, Tector M, Tector AJ. J Immunol. 2014 Dec 1;193(11):5751-7. doi: 10.4049/jimmunol.1402059. Epub 2014 Oct 22. PubMed
Creating cancer translocations in human cells using Cas9 DSBs and nCas9 paired nicks. Renouf B, Piganeau M, Ghezraoui H, Jasin M, Brunet E. Methods Enzymol. 2014;546:251-71. doi: 10.1016/B978-0-12-801185-0.00012-X. PubMed
CRISPR/Cas9-based genome editing in mice by single plasmid injection. Fujihara Y, Ikawa M. Methods Enzymol. 2014;546:319-36. doi: 10.1016/B978-0-12-801185-0.00015-5. PubMed
Genome engineering using CRISPR-Cas9 system. Cong L, Zhang F. Methods Mol Biol. 2015;1239:197-217. doi: 10.1007/978-1-4939-1862-1_10. PubMed
Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9. Nakade S, Tsubota T, Sakane Y, Kume S, Sakamoto N, Obara M, Daimon T, Sezutsu H, Yamamoto T, Sakuma T, Suzuki KT. Nat Commun. 2014 Nov 20;5:5560. doi: 10.1038/ncomms6560. PubMed
The intraflagellar transport protein IFT27 promotes BBSome exit from cilia through the GTPase ARL6/BBS3. Liew GM, Ye F, Nager AR, Murphy JP, Lee JS, Aguiar M, Breslow DK, Gygi SP, Nachury MV. Dev Cell. 2014 Nov 10;31(3):265-78. doi: 10.1016/j.devcel.2014.09.004. Epub 2014 Oct 30. PubMed
Correction of a genetic disease by CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells. Wu Y, Zhou H, Fan X, Zhang Y, Zhang M, Wang Y, Xie Z, Bai M, Yin Q, Liang D, Tang W, Liao J, Zhou C, Liu W, Zhu P, Guo H, Pan H, Wu C, Shi H, Wu L, Tang F, Li J. Cell Res. 2015 Jan;25(1):67-79. doi: 10.1038/cr.2014.160. Epub 2014 Dec 5. PubMed
Generation of WNK1 knockout cell lines by CRISPR/Cas-mediated genome editing. Roy A, Goodman JH, Begum G, Donnelly BF, Pittman G, Weinman EJ, Sun D, Subramanya AR. Am J Physiol Renal Physiol. 2015 Feb 15;308(4):F366-76. doi: 10.1152/ajprenal.00612.2014. Epub 2014 Dec 4. PubMed
Engineering herpes simplex viruses by infection-transfection methods including recombination site targeting by CRISPR/Cas9 nucleases. Russell TA, Stefanovic T, Tscharke DC. J Virol Methods. 2015 Mar;213:18-25. doi: 10.1016/j.jviromet.2014.11.009. Epub 2014 Dec 3. PubMed
ST3Gal-4 is the primary sialyltransferase regulating the synthesis of E-, P-, and L-selectin ligands on human myeloid leukocytes. Mondal N, Buffone A Jr, Stolfa G, Antonopoulos A, Lau JT, Haslam SM, Dell A, Neelamegham S. Blood. 2015 Jan 22;125(4):687-96. doi: 10.1182/blood-2014-07-588590. Epub 2014 Dec 10. PubMed
Amphipathic alpha-helices in apolipoproteins are crucial to the formation of infectious hepatitis C virus particles. Fukuhara T, Wada M, Nakamura S, Ono C, Shiokawa M, Yamamoto S, Motomura T, Okamoto T, Okuzaki D, Yamamoto M, Saito I, Wakita T, Koike K, Matsuura Y. PLoS Pathog. 2014 Dec 11;10(12):e1004534. doi: 10.1371/journal.ppat.1004534. eCollection 2014 Dec. PubMed
Genome-wide detection of DNA double-stranded breaks induced by engineered nucleases. Frock RL, Hu J, Meyers RM, Ho YJ, Kii E, Alt FW. Nat Biotechnol. 2015 Feb;33(2):179-86. doi: 10.1038/nbt.3101. Epub 2014 Dec 15. PubMed
Generation of genomic deletions in mammalian cell lines via CRISPR/Cas9. Bauer DE, Canver MC, Orkin SH. J Vis Exp. 2014 Jan 3;(95). doi: 10.3791/52118. PubMed
Detailed phenotypic and molecular analyses of genetically modified mice generated by CRISPR-Cas9-mediated editing. Parikh BA, Beckman DL, Patel SJ, White JM, Yokoyama WM. PLoS One. 2015 Jan 14;10(1):e0116484. doi: 10.1371/journal.pone.0116484. eCollection 2015. PubMed
Improving gene targeting efficiency on pig IGF2 mediated by ZFNs and CRISPR/Cas9 by using SSA reporter system. Jinqing W, Gui M, Zhiguo L, Yaosheng C, Peiqing C, Zuyong H. Yi Chuan. 2015 Jan;37(1):55-62. doi: 10.16288/j.yczz.2015.01.008. PubMed
Site- and allele-specific polycomb dysregulation in T-cell leukaemia. Navarro JM, Touzart A, Pradel LC, Loosveld M, Koubi M, Fenouil R, Le Noir S, Maqbool MA, Morgado E, Gregoire C, Jaeger S, Mamessier E, Pignon C, Hacein-Bey-Abina S, Malissen B, Gut M, Gut IG, Dombret H, Macintyre EA, Howe SJ, Gaspar HB, Thrasher AJ, Ifrah N, Payet-Bornet D, Duprez E, Andrau JC, Asnafi V, Nadel B. Nat Commun. 2015 Jan 23;6:6094. doi: 10.1038/ncomms7094. PubMed
DNA damage response factors from diverse pathways, including DNA crosslink repair, mediate alternative end joining. Howard SM, Yanez DA, Stark JM. PLoS Genet. 2015 Jan 28;11(1):e1004943. doi: 10.1371/journal.pgen.1004943. eCollection 2015 Jan. PubMed
Mammalian polymerase theta promotes alternative NHEJ and suppresses recombination. Mateos-Gomez PA, Gong F, Nair N, Miller KM, Lazzerini-Denchi E, Sfeir A. Nature. 2015 Feb 12;518(7538):254-7. doi: 10.1038/nature14157. Epub 2015 Feb 2. PubMed Associated Plasmids pX330(puro) Rosa26-H3F3B pcDNA 3.1(Hygro) myc-hPolQ-Flag
Modeling of the human alveolar rhabdomyosarcoma Pax3-Foxo1 chromosome translocation in mouse myoblasts using CRISPR-Cas9 nuclease. Lagutina IV, Valentine V, Picchione F, Harwood F, Valentine MB, Villarejo-Balcells B, Carvajal JJ, Grosveld GC. PLoS Genet. 2015 Feb 6;11(2):e1004951. doi: 10.1371/journal.pgen.1004951. eCollection 2015. PubMed
Highly efficient CRISPR/Cas9-mediated TAR cloning of genes and chromosomal loci from complex genomes in yeast. Lee NC, Larionov V, Kouprina N. Nucleic Acids Res. 2015 Apr 30;43(8):e55. doi: 10.1093/nar/gkv112. Epub 2015 Feb 17. PubMed
Evaluation of human and non-human primate antibody binding to pig cells lacking GGTA1/CMAH/beta4GalNT2 genes. Estrada JL, Martens G, Li P, Adams A, Newell KA, Ford ML, Butler JR, Sidner R, Tector M, Tector J. Xenotransplantation. 2015 May;22(3):194-202. doi: 10.1111/xen.12161. Epub 2015 Mar 1. PubMed
Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish. Hisano Y, Sakuma T, Nakade S, Ohga R, Ota S, Okamoto H, Yamamoto T, Kawahara A. Sci Rep. 2015 Mar 5;5:8841. doi: 10.1038/srep08841. PubMed
Hepatitis C virus RNA functionally sequesters miR-122. Luna JM, Scheel TK, Danino T, Shaw KS, Mele A, Fak JJ, Nishiuchi E, Takacs CN, Catanese MT, de Jong YP, Jacobson IM, Rice CM, Darnell RB. Cell. 2015 Mar 12;160(6):1099-110. doi: 10.1016/j.cell.2015.02.025. PubMed
Efficient human immunodeficiency virus (HIV-1) infection of cells lacking PDZD8. Zhang S, Sodroski J. Virology. 2015 Jul;481:73-8. doi: 10.1016/j.virol.2015.01.034. Epub 2015 Mar 11. PubMed
Selective Distal Enhancer Control of the Mmp13 Gene Identified through Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) Genomic Deletions. Meyer MB, Benkusky NA, Pike JW. J Biol Chem. 2015 Apr 24;290(17):11093-107. doi: 10.1074/jbc.M115.648394. Epub 2015 Mar 15. PubMed
Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining. Maruyama T, Dougan SK, Truttmann MC, Bilate AM, Ingram JR, Ploegh HL. Nat Biotechnol. 2015 May;33(5):538-42. doi: 10.1038/nbt.3190. Epub 2015 Mar 23. PubMed
Convergence of developmental and oncogenic signaling pathways at transcriptional super-enhancers. Hnisz D, Schuijers J, Lin CY, Weintraub AS, Abraham BJ, Lee TI, Bradner JE, Young RA. Mol Cell. 2015 Apr 16;58(2):362-70. doi: 10.1016/j.molcel.2015.02.014. Epub 2015 Mar 19. PubMed
Large genomic fragment deletions and insertions in mouse using CRISPR/Cas9. Zhang L, Jia R, Palange NJ, Satheka AC, Togo J, An Y, Humphrey M, Ban L, Ji Y, Jin H, Feng X, Zheng Y. PLoS One. 2015 Mar 24;10(3):e0120396. doi: 10.1371/journal.pone.0120396. eCollection 2015. PubMed
CRISPR/Cas9 nuclease cleavage combined with Gibson assembly for seamless cloning. Wang JW, Wang A, Li K, Wang B, Jin S, Reiser M, Lockey RF. Biotechniques. 2015 Apr 1;58(4):161-70. doi: 10.2144/000114261. eCollection 2015 Apr. PubMed
TRAF Family Member-Associated NF-kappaB Activator Inhibits Genotoxic NF-kappaB Activation by Facilitating USP10-Dependent Deubiquitination of TRAF6. Wang W, Huang X, Xin HB, Fu M, Xue A, Wu ZH. J Biol Chem. 2015 Apr 10. pii: jbc.M115.643767. PubMed
CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes. Liang P, Xu Y, Zhang X, Ding C, Huang R, Zhang Z, Lv J, Xie X, Chen Y, Li Y, Sun Y, Bai Y, Songyang Z, Ma W, Zhou C, Huang J. Protein Cell. 2015 May;6(5):363-72. doi: 10.1007/s13238-015-0153-5. Epub 2015 Apr 18. PubMed
Integrative Analysis of CRISPR/Cas9 Target Sites in the Human HBB Gene. Luo Y, Zhu D, Zhang Z, Chen Y, Sun X. Biomed Res Int. 2015;2015:514709. doi: 10.1155/2015/514709. Epub 2015 Mar 31. PubMed
Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice. Aida T, Chiyo K, Usami T, Ishikubo H, Imahashi R, Wada Y, Tanaka KF, Sakuma T, Yamamoto T, Tanaka K. Genome Biol. 2015 Apr 29;16:87. doi: 10.1186/s13059-015-0653-x. PubMed
A Rapid Embryonic Stem Cell-Based Mouse Model for B-cell Lymphomas Driven by Epstein-Barr Virus Protein LMP1. Ba Z, Meng FL, Gostissa M, Huang PY, Ke Q, Wang Z, Dao MN, Fujiwara Y, Rajewsky K, Zhang B, Alt FW. Cancer Immunol Res. 2015 Jun;3(6):641-9. doi: 10.1158/2326-6066.CIR-15-0058. Epub 2015 May 1. PubMed
Efficient CRISPR-Cas9-mediated generation of knockin human pluripotent stem cells lacking undesired mutations at the targeted locus. Merkle FT, Neuhausser WM, Santos D, Valen E, Gagnon JA, Maas K, Sandoe J, Schier AF, Eggan K. Cell Rep. 2015 May 12;11(6):875-83. doi: 10.1016/j.celrep.2015.04.007. Epub 2015 Apr 30. PubMed
DNA Sequence-Specific Binding of CENP-B Enhances the Fidelity of Human Centromere Function. Fachinetti D, Han JS, McMahon MA, Ly P, Abdullah A, Wong AJ, Cleveland DW. Dev Cell. 2015 May 4;33(3):314-27. doi: 10.1016/j.devcel.2015.03.020. PubMed
Advantages of using the CRISPR/Cas9 system of genome editing to investigate male reproductive mechanisms using mouse models. Young SA, Aitken RJ, Ikawa M. Asian J Androl. 2015 Jul-Aug;17(4):623-7. doi: 10.4103/1008-682X.153851. PubMed
Foxp1 regulates cortical radial migration and neuronal morphogenesis in developing cerebral cortex. Li X, Xiao J, Frohlich H, Tu X, Li L, Xu Y, Cao H, Qu J, Rappold GA, Chen JG. PLoS One. 2015 May 26;10(5):e0127671. doi: 10.1371/journal.pone.0127671. eCollection 2015. PubMed
CRISPR/Cas9-mediated reporter knock-in in mouse haploid embryonic stem cells. Kimura Y, Oda M, Nakatani T, Sekita Y, Monfort A, Wutz A, Mochizuki H, Nakano T. Sci Rep. 2015 Jun 3;5:10710. doi: 10.1038/srep10710. PubMed
Regulation of Gene Editing Activity Directed by Single-Stranded Oligonucleotides and CRISPR/Cas9 Systems. Bialk P, Rivera-Torres N, Strouse B, Kmiec EB. PLoS One. 2015 Jun 8;10(6):e0129308. doi: 10.1371/journal.pone.0129308. eCollection 2015. PubMed
Photoactivatable CRISPR-Cas9 for optogenetic genome editing. Nihongaki Y, Kawano F, Nakajima T, Sato M. Nat Biotechnol. 2015 Jul;33(7):755-60. doi: 10.1038/nbt.3245. Epub 2015 Jun 15. PubMed
Biological Roles of Hydroxysteroid (11-Beta) Dehydrogenase 1 (HSD11B1), HSD11B2, and Glucocorticoid Receptor (NR3C1) in Sheep Conceptus Elongation. Brooks K, Burns G, Spencer TE. Biol Reprod. 2015 Aug;93(2):38. doi: 10.1095/biolreprod.115.130757. Epub 2015 Jun 17. PubMed
Transcription Factor ATF4 Induces NLRP1 Inflammasome Expression during Endoplasmic Reticulum Stress. D'Osualdo A, Anania VG, Yu K, Lill JR, Kaufman RJ, Matsuzawa S, Reed JC. PLoS One. 2015 Jun 18;10(6):e0130635. doi: 10.1371/journal.pone.0130635. eCollection 2015. PubMed
CRISPR-mediated genotypic and phenotypic correction of a chronic granulomatous disease mutation in human iPS cells. Flynn R, Grundmann A, Renz P, Hanseler W, James WS, Cowley SA, Moore MD. Exp Hematol. 2015 Oct;43(10):838-848.e3. doi: 10.1016/j.exphem.2015.06.002. Epub 2015 Jun 19. PubMed
Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells. Hendel A, Bak RO, Clark JT, Kennedy AB, Ryan DE, Roy S, Steinfeld I, Lunstad BD, Kaiser RJ, Wilkens AB, Bacchetta R, Tsalenko A, Dellinger D, Bruhn L, Porteus MH. Nat Biotechnol. 2015 Sep;33(9):985-9. doi: 10.1038/nbt.3290. Epub 2015 Jun 29. PubMed
An autoinhibitory mechanism modulates MAVS activity in antiviral innate immune response. Shi Y, Yuan B, Qi N, Zhu W, Su J, Li X, Qi P, Zhang D, Hou F. Nat Commun. 2015 Jul 17;6:7811. doi: 10.1038/ncomms8811. PubMed
Identification of Spen as a Crucial Factor for Xist Function through Forward Genetic Screening in Haploid Embryonic Stem Cells. Monfort A, Di Minin G, Postlmayr A, Freimann R, Arieti F, Thore S, Wutz A. Cell Rep. 2015 Jul 28;12(4):554-61. doi: 10.1016/j.celrep.2015.06.067. Epub 2015 Jul 16. PubMed
A CRISPR-Based Screen Identifies Genes Essential for West-Nile-Virus-Induced Cell Death. Ma H, Dang Y, Wu Y, Jia G, Anaya E, Zhang J, Abraham S, Choi JG, Shi G, Qi L, Manjunath N, Wu H. Cell Rep. 2015 Jul 28;12(4):673-83. doi: 10.1016/j.celrep.2015.06.049. Epub 2015 Jul 16. PubMed Associated Plasmids Human lentiviral CRISPR library_v1
Pathogenesis of ELANE-mutant severe neutropenia revealed by induced pluripotent stem cells. Nayak RC, Trump LR, Aronow BJ, Myers K, Mehta P, Kalfa T, Wellendorf AM, Valencia CA, Paddison PJ, Horwitz MS, Grimes HL, Lutzko C, Cancelas JA. J Clin Invest. 2015 Aug 3;125(8):3103-16. doi: 10.1172/JCI80924. Epub 2015 Jul 20. PubMed
Double strand break repair by capture of retrotransposon sequences and reverse-transcribed spliced mRNA sequences in mouse zygotes. Ono R, Ishii M, Fujihara Y, Kitazawa M, Usami T, Kaneko-Ishino T, Kanno J, Ikawa M, Ishino F. Sci Rep. 2015 Jul 28;5:12281. doi: 10.1038/srep12281. PubMed
CFTR inactivation by lentiviral vector-mediated RNA interference and CRISPR-Cas9 genome editing in human airway epithelial cells. Bellec J, Bacchetta M, Losa D, Anegon I, Chanson M, Nguyen TH. Curr Gene Ther. 2015;15(5):447-59. PubMed
Efficient CRISPR/Cas9-mediated biallelic gene disruption and site-specific knockin after rapid selection of highly active sgRNAs in pigs. Wang X, Zhou J, Cao C, Huang J, Hai T, Wang Y, Zheng Q, Zhang H, Qin G, Miao X, Wang H, Cao S, Zhou Q, Zhao J. Sci Rep. 2015 Aug 21;5:13348. doi: 10.1038/srep13348. PubMed Associated Plasmids pX330-mitf
Efficient Generation of Myostatin Knock-Out Sheep Using CRISPR/Cas9 Technology and Microinjection into Zygotes. Crispo M, Mulet AP, Tesson L, Barrera N, Cuadro F, dos Santos-Neto PC, Nguyen TH, Creneguy A, Brusselle L, Anegon I, Menchaca A. PLoS One. 2015 Aug 25;10(8):e0136690. doi: 10.1371/journal.pone.0136690. eCollection 2015. PubMed
Orientation-specific joining of AID-initiated DNA breaks promotes antibody class switching. Dong J, Panchakshari RA, Zhang T, Zhang Y, Hu J, Volpi SA, Meyers RM, Ho YJ, Du Z, Robbiani DF, Meng F, Gostissa M, Nussenzweig MC, Manis JP, Alt FW. Nature. 2015 Sep 3;525(7567):134-9. doi: 10.1038/nature14970. Epub 2015 Aug 26. PubMed
Novel reporter system to monitor early stages of the hepatitis B virus life cycle. Nishitsuji H, Ujino S, Shimizu Y, Harada K, Zhang J, Sugiyama M, Mizokami M, Shimotohno K. Cancer Sci. 2015 Nov;106(11):1616-24. doi: 10.1111/cas.12799. Epub 2015 Oct 16. PubMed
EHMT1 and EHMT2 inhibition induces fetal hemoglobin expression. Renneville A, Van Galen P, Canver MC, McConkey M, Krill-Burger JM, Dorfman DM, Holson EB, Bernstein BE, Orkin SH, Bauer DE, Ebert BL. Blood. 2015 Oct 15;126(16):1930-9. doi: 10.1182/blood-2015-06-649087. Epub 2015 Aug 28. PubMed
Efficient genetic engineering of human intestinal organoids using electroporation. Fujii M, Matano M, Nanki K, Sato T. Nat Protoc. 2015 Oct;10(10):1474-85. doi: 10.1038/nprot.2015.088. Epub 2015 Sep 3. PubMed
Peri-implantation lethality in mice carrying megabase-scale deletion on 5qc3.3 is caused by Exoc1 null mutation. Mizuno S, Takami K, Daitoku Y, Tanimoto Y, Dinh TT, Mizuno-Iijima S, Hasegawa Y, Takahashi S, Sugiyama F, Yagami K. Sci Rep. 2015 Sep 8;5:13632. doi: 10.1038/srep13632. PubMed
RORalpha Regulates Multiple Aspects of Dendrite Development in Cerebellar Purkinje Cells In Vivo. Takeo YH, Kakegawa W, Miura E, Yuzaki M. J Neurosci. 2015 Sep 9;35(36):12518-34. doi: 10.1523/JNEUROSCI.0075-15.2015. PubMed
Molecular basis underlying resistance to Mps1/TTK inhibitors. Koch A, Maia A, Janssen A, Medema RH. Oncogene. 2015 Sep 14. doi: 10.1038/onc.2015.319. PubMed
BCL11A enhancer dissection by Cas9-mediated in situ saturating mutagenesis. Canver MC, Smith EC, Sher F, Pinello L, Sanjana NE, Shalem O, Chen DD, Schupp PG, Vinjamur DS, Garcia SP, Luc S, Kurita R, Nakamura Y, Fujiwara Y, Maeda T, Yuan GC, Zhang F, Orkin SH, Bauer DE. Nature. 2015 Sep 16. doi: 10.1038/nature15521. PubMed Associated Plasmids lentiCas9-Venus lentiGuide-Crimson
Tracking and transforming neocortical progenitors by CRISPR/Cas9 gene targeting and piggyBac transposase lineage labeling. Chen F, Rosiene J, Che A, Becker A, LoTurco J. Development. 2015 Oct 15;142(20):3601-11. doi: 10.1242/dev.118836. Epub 2015 Sep 23. PubMed
HIV-1 Nef promotes infection by excluding SERINC5 from virion incorporation. Rosa A, Chande A, Ziglio S, De Sanctis V, Bertorelli R, Goh SL, McCauley SM, Nowosielska A, Antonarakis SE, Luban J, Santoni FA, Pizzato M. Nature. 2015 Oct 8;526(7572):212-7. doi: 10.1038/nature15399. Epub 2015 Sep 30. PubMed
The lethal response to Cdk1 inhibition depends on sister chromatid alignment errors generated by KIF4 and isoform 1 of PRC1. Voets E, Marsman J, Demmers J, Beijersbergen R, Wolthuis R. Sci Rep. 2015 Oct 1;5:14798. doi: 10.1038/srep14798. PubMed
gp78 functions downstream of Hrd1 to promote degradation of misfolded proteins of the endoplasmic reticulum. Zhang T, Xu Y, Liu Y, Ye Y. Mol Biol Cell. 2015 Dec 1;26(24):4438-50. doi: 10.1091/mbc.E15-06-0354. Epub 2015 Sep 30. PubMed
Nuclear domain 'knock-in' screen for the evaluation and identification of small molecule enhancers of CRISPR-based genome editing. Pinder J, Salsman J, Dellaire G. Nucleic Acids Res. 2015 Oct 30;43(19):9379-92. doi: 10.1093/nar/gkv993. Epub 2015 Oct 1. PubMed Associated Plasmids pX330-LMNA-gRNA1 pCR2.1 Clover-LMNA Donor
Orthogonal gene knockout and activation with a catalytically active Cas9 nuclease. Dahlman JE, Abudayyeh OO, Joung J, Gootenberg JS, Zhang F, Konermann S. Nat Biotechnol. 2015 Oct 5;33(11):1159-1161. doi: 10.1038/nbt.3390. PubMed
CRISPR/Cas9-Mediated Rapid Generation of Multiple Mouse Lines Identified Ccdc63 as Essential for Spermiogenesis. Young SA, Miyata H, Satouh Y, Kato H, Nozawa K, Isotani A, Aitken RJ, Baker MA, Ikawa M. Int J Mol Sci. 2015 Oct 16;16(10):24732-50. doi: 10.3390/ijms161024732. PubMed
Identification of a novel cis-regulatory element essential for immune tolerance. LaFlam TN, Seumois G, Miller CN, Lwin W, Fasano KJ, Waterfield M, Proekt I, Vijayanand P, Anderson MS. J Exp Med. 2015 Nov 16;212(12):1993-2002. doi: 10.1084/jem.20151069. Epub 2015 Nov 2. PubMed
MITF and c-Jun antagonism interconnects melanoma dedifferentiation with pro-inflammatory cytokine responsiveness and myeloid cell recruitment. Riesenberg S, Groetchen A, Siddaway R, Bald T, Reinhardt J, Smorra D, Kohlmeyer J, Renn M, Phung B, Aymans P, Schmidt T, Hornung V, Davidson I, Goding CR, Jonsson G, Landsberg J, Tuting T, Holzel M. Nat Commun. 2015 Nov 4;6:8755. doi: 10.1038/ncomms9755. PubMed
IRE1alpha is an endogenous substrate of endoplasmic-reticulum-associated degradation. Sun S, Shi G, Sha H, Ji Y, Han X, Shu X, Ma H, Inoue T, Gao B, Kim H, Bu P, Guber RD, Shen X, Lee AH, Iwawaki T, Paton AW, Paton JC, Fang D, Tsai B, Yates JR 3rd, Wu H, Kersten S, Long Q, Duhamel GE, Simpson KW, Qi L. Nat Cell Biol. 2015 Dec;17(12):1546-55. doi: 10.1038/ncb3266. Epub 2015 Nov 9. PubMed
Efficient Generation of Myostatin Mutations in Pigs Using the CRISPR/Cas9 System. Wang K, Ouyang H, Xie Z, Yao C, Guo N, Li M, Jiao H, Pang D. Sci Rep. 2015 Nov 13;5:16623. doi: 10.1038/srep16623. PubMed
A forward genetic screen reveals novel independent regulators of ULBP1, an activating ligand for natural killer cells. Gowen BG, Chim B, Marceau CD, Greene TT, Burr P, Gonzalez JR, Hesser CR, Dietzen PA, Russell T, Iannello A, Coscoy L, Sentman CL, Carette JE, Muljo SA, Raulet DH. Elife. 2015 Nov 13;4. pii: e08474. doi: 10.7554/eLife.08474. PubMed
Involvement of FKBP6 in hepatitis C virus replication. Kasai H, Kawakami K, Yokoe H, Yoshimura K, Matsuda M, Yasumoto J, Maekawa S, Yamashita A, Tanaka T, Ikeda M, Kato N, Okamoto T, Matsuura Y, Sakamoto N, Enomoto N, Takeda S, Fujii H, Tsubuki M, Kusunoki M, Moriishi K. Sci Rep. 2015 Nov 16;5:16699. doi: 10.1038/srep16699. PubMed
Definitive localization of intracellular proteins: Novel approach using CRISPR-Cas9 genome editing, with glucose 6-phosphate dehydrogenase as a model. Spencer NY, Yan Z, Cong L, Zhang Y, Engelhardt JF, Stanton RC. Anal Biochem. 2016 Feb 1;494:55-67. doi: 10.1016/j.ab.2015.11.002. Epub 2015 Nov 11. PubMed
Depletion of elongation initiation factor 4E binding proteins by CRISPR/Cas9 enhances the antiviral response in porcine cells. Ramirez-Carvajal L, Singh N, de Los Santos T, Rodriguez LL, Long CR. Antiviral Res. 2016 Jan;125:8-13. doi: 10.1016/j.antiviral.2015.11.002. Epub 2015 Nov 22. PubMed
CRISPR/Cas9 Promotes Functional Study of Testis Specific X-Linked Gene In Vivo. Li M, Huang R, Jiang X, Chen Y, Zhang Z, Zhang X, Liang P, Zhan S, Cao S, Songyang Z, Huang J. PLoS One. 2015 Nov 24;10(11):e0143148. doi: 10.1371/journal.pone.0143148. eCollection 2015. PubMed
Large genomic fragment deletion and functional gene cassette knock-in via Cas9 protein mediated genome editing in one-cell rodent embryos. Wang L, Shao Y, Guan Y, Li L, Wu L, Chen F, Liu M, Chen H, Ma Y, Ma X, Liu M, Li D. Sci Rep. 2015 Dec 1;5:17517. doi: 10.1038/srep17517. PubMed
Production of functional small interfering RNAs by an amino-terminal deletion mutant of human Dicer. Kennedy EM, Whisnant AW, Kornepati AV, Marshall JB, Bogerd HP, Cullen BR. Proc Natl Acad Sci U S A. 2015 Dec 15;112(50):E6945-54. doi: 10.1073/pnas.1513421112. Epub 2015 Nov 30. PubMed
The experimental power of FR900359 to study Gq-regulated biological processes. Schrage R, Schmitz AL, Gaffal E, Annala S, Kehraus S, Wenzel D, Bullesbach KM, Bald T, Inoue A, Shinjo Y, Galandrin S, Shridhar N, Hesse M, Grundmann M, Merten N, Charpentier TH, Martz M, Butcher AJ, Slodczyk T, Armando S, Effern M, Namkung Y, Jenkins L, Horn V, Stossel A, Dargatz H, Tietze D, Imhof D, Gales C, Drewke C, Muller CE, Holzel M, Milligan G, Tobin AB, Gomeza J, Dohlman HG, Sondek J, Harden TK, Bouvier M, Laporte SA, Aoki J, Fleischmann BK, Mohr K, Konig GM, Tuting T, Kostenis E. Nat Commun. 2015 Dec 14;6:10156. doi: 10.1038/ncomms10156. PubMed
Optimizing sgRNA structure to improve CRISPR-Cas9 knockout efficiency. Dang Y, Jia G, Choi J, Ma H, Anaya E, Ye C, Shankar P, Wu H. Genome Biol. 2015 Dec 15;16:280. doi: 10.1186/s13059-015-0846-3. PubMed
MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems. Sakuma T, Nakade S, Sakane Y, Suzuki KT, Yamamoto T. Nat Protoc. 2016 Jan;11(1):118-33. doi: 10.1038/nprot.2015.140. Epub 2015 Dec 17. PubMed Associated Plasmids pX330S-2-PITCh pX330A-FBL/PITCh pCRIS-PITChv2-FBL
Quantification of Protein Levels in Single Living Cells. Lo CA, Kays I, Emran F, Lin TJ, Cvetkovska V, Chen BE. Cell Rep. 2015 Dec 22;13(11):2634-44. doi: 10.1016/j.celrep.2015.11.048. Epub 2015 Dec 10. PubMed Associated Plasmids pCAG-GFP-PQRv3-RFP pJFRC7-RFPnls-PQRv2-mCD8:GFP
The bimodally expressed microRNA miR-142 gates exit from pluripotency. Sladitschek HL, Neveu PA. Mol Syst Biol. 2015 Dec 21;11(12):850. doi: 10.15252/msb.20156525. PubMed
Hepatitis C virus utilizes VLDLR as a novel entry pathway. Ujino S, Nishitsuji H, Hishiki T, Sugiyama K, Takaku H, Shimotohno K. Proc Natl Acad Sci U S A. 2016 Jan 5;113(1):188-93. doi: 10.1073/pnas.1506524113. Epub 2015 Dec 23. PubMed
The splicing regulator PTBP1 controls the activity of the transcription factor Pbx1 during neuronal differentiation. Linares AJ, Lin CH, Damianov A, Adams KL, Novitch BG, Black DL. Elife. 2015 Dec 24;4. pii: e09268. doi: 10.7554/eLife.09268. PubMed
A possible aid in targeted insertion of large DNA elements by CRISPR/Cas in mouse zygotes. Nakao H, Harada T, Nakao K, Kiyonari H, Inoue K, Furuta Y, Aiba A. Genesis. 2015 Dec 29. doi: 10.1002/dvg.22914. PubMed
A mouse model for adult cardiac-specific gene deletion with CRISPR/Cas9. Carroll KJ, Makarewich CA, McAnally J, Anderson DM, Zentilin L, Liu N, Giacca M, Bassel-Duby R, Olson EN. Proc Natl Acad Sci U S A. 2016 Jan 12;113(2):338-43. doi: 10.1073/pnas.1523918113. Epub 2015 Dec 30. PubMed
Efficient CRISPR/Cas9-Based Genome Engineering in Human Pluripotent Stem Cells. Kime C, Mandegar MA, Srivastava D, Yamanaka S, Conklin BR, Rand TA. Curr Protoc Hum Genet. 2016 Jan 1;88:21.4.1-21.4.23. doi: 10.1002/0471142905.hg2104s88. PubMed
Characterization of vitamin K-dependent carboxylase mutations that cause bleeding and non-bleeding disorders. Tie JK, Carneiro JD, Jin DY, Martinhago CD, Vermeer C, Stafford DW. Blood. 2016 Jan 12. pii: blood-2015-10-677633. PubMed
In vivo blunt-end cloning through CRISPR/Cas9-facilitated non-homologous end-joining. Geisinger JM, Turan S, Hernandez S, Spector LP, Calos MP. Nucleic Acids Res. 2016 Jan 13. pii: gkv1542. PubMed Associated Plasmids pX330-BsaIx2-DD-Cas9 pKER-Clover pKER-mKOk pKER-mCardinal pKER-mRuby2 More ...
Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in C57BL/6 zygotes. Chu VT, Weber T, Graf R, Sommermann T, Petsch K, Sack U, Volchkov P, Rajewsky K, Kuhn R. BMC Biotechnol. 2016 Jan 16;16(1):4. doi: 10.1186/s12896-016-0234-4. PubMed Associated Plasmids pR26 CAG/GFP Dest pR26 CAG/BFP Dest pR26 GFP Dest pR26 iBFP Dest pR26 CAG/GFP Asc More ...
Comparison of surrogate reporter systems for enrichment of cells with mutations induced by genome editors. He Z, Shi X, Liu M, Sun G, Proudfoot C, Whitelaw CB, Lillico SG, Chen Y. J Biotechnol. 2016 Jan 14;221:49-54. doi: 10.1016/j.jbiotec.2016.01.009. PubMed
The Neisseria meningitidis CRISPR-Cas9 System Enables Specific Genome Editing in Mammalian Cells. Lee CM, Cradick TJ, Bao G. Mol Ther. 2016 Mar;24(3):645-54. doi: 10.1038/mt.2016.8. Epub 2016 Jan 19. PubMed
Ligase I and ligase III mediate the DNA double-strand break ligation in alternative end-joining. Lu G, Duan J, Shu S, Wang X, Gao L, Guo J, Zhang Y. Proc Natl Acad Sci U S A. 2016 Feb 2;113(5):1256-60. doi: 10.1073/pnas.1521597113. Epub 2016 Jan 19. PubMed
Hepatocyte factor JMJD5 regulates HBV replication through interaction with HBx. Kouwaki T, Okamoto T, Ito A, Sugiyama Y, Yamashita K, Suzuki T, Kusakabe S, Hirano J, Fukuhara T, Yamashita A, Saito K, Okuzaki D, Watashi K, Sugiyama M, Yoshio S, Standley DM, Kanto T, Mizokami M, Moriishi K, Matsuura Y. J Virol. 2016 Jan 20. pii: JVI.02776-15. PubMed
Sequence features associated with the cleavage efficiency of CRISPR/Cas9 system. Liu X, Homma A, Sayadi J, Yang S, Ohashi J, Takumi T. Sci Rep. 2016 Jan 27;6:19675. doi: 10.1038/srep19675. PubMed
A new class of temporarily phenotypic enhancers identified by CRISPR/Cas9-mediated genetic screening. Diao Y, Li B, Meng Z, Jung I, Lee AY, Dixon J, Maliskova L, Guan KL, Shen Y, Ren B. Genome Res. 2016 Mar;26(3):397-405. doi: 10.1101/gr.197152.115. Epub 2016 Jan 26. PubMed
An essential receptor for adeno-associated virus infection. Pillay S, Meyer NL, Puschnik AS, Davulcu O, Diep J, Ishikawa Y, Jae LT, Wosen JE, Nagamine CM, Chapman MS, Carette JE. Nature. 2016 Feb 4;530(7588):108-12. doi: 10.1038/nature16465. Epub 2016 Jan 27. PubMed Associated Plasmids pLenti-CMV-Puro-AAVR-FLAG pLenti-CMV-Puro-miniAAVR-FLAG
Selectable one-step PCR-mediated integration of a degron for rapid depletion of endogenous human proteins. Sheridan RM, Bentley DL. Biotechniques. 2016 Feb 1;60(2):69-74. doi: 10.2144/000114378. eCollection 2016 Feb. PubMed Associated Plasmids pAc5 HA3-eDHFR-T2A-puro pAc5 HA3-eDHFR-T2A-neo
A Burden of Rare Variants Associated with Extremes of Gene Expression in Human Peripheral Blood. Zhao J, Akinsanmi I, Arafat D, Cradick TJ, Lee CM, Banskota S, Marigorta UM, Bao G, Gibson G. Am J Hum Genet. 2016 Feb 4;98(2):299-309. doi: 10.1016/j.ajhg.2015.12.023. PubMed
A Vector with a Single Promoter for In Vitro Transcription and Mammalian Cell Expression of CRISPR gRNAs. Romanienko PJ, Giacalone J, Ingenito J, Wang Y, Isaka M, Johnson T, You Y, Mark WH. PLoS One. 2016 Feb 5;11(2):e0148362. doi: 10.1371/journal.pone.0148362. eCollection 2016. PubMed Associated Plasmids pU6T7 pU6T7G
Post-translational Regulation of Cas9 during G1 Enhances Homology-Directed Repair. Gutschner T, Haemmerle M, Genovese G, Draetta GF, Chin L. Cell Rep. 2016 Feb 3. pii: S2211-1247(16)00040-1. doi: 10.1016/j.celrep.2016.01.019. PubMed Associated Plasmids pX330-U6-Chimeric_BB-CBh-hSpCas9-hGem(1/110)
Targeted Gene Deletion Using DNA-Free RNA-Guided Cas9 Nuclease Accelerates Adaptation of CHO Cells to Suspension Culture. Lee N, Shin J, Park JH, Lee GM, Cho S, Cho BK. ACS Synth Biol. 2016 Nov 18;5(11):1211-1219. Epub 2016 Feb 18. PubMed
CRISPR/Cas9-mediated gene knockout in the mouse brain using in utero electroporation. Shinmyo Y, Tanaka S, Tsunoda S, Hosomichi K, Tajima A, Kawasaki H. Sci Rep. 2016 Feb 9;6:20611. doi: 10.1038/srep20611. PubMed
Structure-function analysis of myomaker domains required for myoblast fusion. Millay DP, Gamage DG, Quinn ME, Min YL, Mitani Y, Bassel-Duby R, Olson EN. Proc Natl Acad Sci U S A. 2016 Feb 23;113(8):2116-21. doi: 10.1073/pnas.1600101113. Epub 2016 Feb 8. PubMed
Immunomodulatory drugs inhibit TLR4-induced type-1 interferon production independently of Cereblon via suppression of the TRIF/IRF3 pathway. Millrine D, Miyata H, Tei M, Dubey P, Nyati K, Nakahama T, Gemechu Y, Ripley B, Kishimoto T. Int Immunol. 2016 Jun;28(6):307-15. doi: 10.1093/intimm/dxw005. Epub 2016 Feb 10. PubMed
Glycosphingolipids on Human Myeloid Cells Stabilize E-Selectin-Dependent Rolling in the Multistep Leukocyte Adhesion Cascade. Mondal N, Stolfa G, Antonopoulos A, Zhu Y, Wang SS, Buffone A Jr, Atilla-Gokcumen GE, Haslam SM, Dell A, Neelamegham S. Arterioscler Thromb Vasc Biol. 2016 Apr;36(4):718-27. doi: 10.1161/ATVBAHA.115.306748. Epub 2016 Feb 11. PubMed
Transcription-associated processes cause DNA double-strand breaks and translocations in neural stem/progenitor cells. Schwer B, Wei PC, Chang AN, Kao J, Du Z, Meyers RM, Alt FW. Proc Natl Acad Sci U S A. 2016 Feb 23;113(8):2258-63. doi: 10.1073/pnas.1525564113. Epub 2016 Feb 12. PubMed
Highly Efficient CRISPR/Cas9-Mediated Cloning and Functional Characterization of Gastric Cancer-Derived Epstein-Barr Virus Strains. Kanda T, Furuse Y, Oshitani H, Kiyono T. J Virol. 2016 Apr 14;90(9):4383-93. doi: 10.1128/JVI.00060-16. Print 2016 May 1. PubMed
Precise Correction of Disease Mutations in Induced Pluripotent Stem Cells Derived From Patients With Limb Girdle Muscular Dystrophy. Turan S, Farruggio AP, Srifa W, Day JW, Calos MP. Mol Ther. 2016 Apr;24(4):685-96. doi: 10.1038/mt.2016.40. Epub 2016 Feb 26. PubMed
Multiplexed pancreatic genome engineering and cancer induction by transfection-based CRISPR/Cas9 delivery in mice. Maresch R, Mueller S, Veltkamp C, Ollinger R, Friedrich M, Heid I, Steiger K, Weber J, Engleitner T, Barenboim M, Klein S, Louzada S, Banerjee R, Strong A, Stauber T, Gross N, Geumann U, Lange S, Ringelhan M, Varela I, Unger K, Yang F, Schmid RM, Vassiliou GS, Braren R, Schneider G, Heikenwalder M, Bradley A, Saur D, Rad R. Nat Commun. 2016 Feb 26;7:10770. doi: 10.1038/ncomms10770. PubMed
A RUNX2-Mediated Epigenetic Regulation of the Survival of p53 Defective Cancer Cells. Shin MH, He Y, Marrogi E, Piperdi S, Ren L, Khanna C, Gorlick R, Liu C, Huang J. PLoS Genet. 2016 Feb 29;12(2):e1005884. doi: 10.1371/journal.pgen.1005884. eCollection 2016 Feb. PubMed
Generating Mouse Models Using CRISPR-Cas9-Mediated Genome Editing. Qin W, Kutny PM, Maser RS, Dion SL, Lamont JD, Zhang Y, Perry GA, Wang H. Curr Protoc Mouse Biol. 2016 Mar 1;6(1):39-66. doi: 10.1002/9780470942390.mo150178. PubMed
Structural and Functional Analysis of a Novel Interaction Motif within UFM1-activating Enzyme 5 (UBA5) Required for Binding to Ubiquitin-like Proteins and Ufmylation. Habisov S, Huber J, Ichimura Y, Akutsu M, Rogova N, Loehr F, McEwan DG, Johansen T, Dikic I, Doetsch V, Komatsu M, Rogov VV, Kirkin V. J Biol Chem. 2016 Apr 22;291(17):9025-41. doi: 10.1074/jbc.M116.715474. Epub 2016 Feb 29. PubMed
Cancer therapeutic approach based on conformational stabilization of mutant p53 protein by small peptides. Tal P, Eizenberger S, Cohen E, Goldfinger N, Pietrokovski S, Oren M, Rotter V. Oncotarget. 2016 Mar 15;7(11):11817-37. doi: 10.18632/oncotarget.7857. PubMed
ECEL1 mutation implicates impaired axonal arborization of motor nerves in the pathogenesis of distal arthrogryposis. Nagata K, Kiryu-Seo S, Tamada H, Okuyama-Uchimura F, Kiyama H, Saido TC. Acta Neuropathol. 2016 Jul;132(1):111-26. doi: 10.1007/s00401-016-1554-0. Epub 2016 Mar 7. PubMed
Pathologic activation of thrombopoietin receptor and JAK2-STAT5 pathway by frameshift mutants of mouse calreticulin. Balligand T, Achouri Y, Pecquet C, Chachoua I, Nivarthi H, Marty C, Vainchenker W, Plo I, Kralovics R, Constantinescu SN. Leukemia. 2016 Feb 29. doi: 10.1038/leu.2016.47. PubMed
Modeling Smith-Lemli-Opitz syndrome with induced pluripotent stem cells reveals a causal role for Wnt/beta-catenin defects in neuronal cholesterol synthesis phenotypes. Francis KR, Ton AN, Xin Y, O'Halloran PE, Wassif CA, Malik N, Williams IM, Cluzeau CV, Trivedi NS, Pavan WJ, Cho W, Westphal H, Porter FD. Nat Med. 2016 Apr;22(4):388-96. doi: 10.1038/nm.4067. Epub 2016 Mar 21. PubMed
Immunoblot screening of CRISPR/Cas9-mediated gene knockouts without selection. Estep JA, Sternburg EL, Sanchez GA, Karginov FV. BMC Mol Biol. 2016 Apr 2;17:9. doi: 10.1186/s12867-016-0061-0. PubMed
Unlinking an lncRNA from Its Associated cis Element. Paralkar VR, Taborda CC, Huang P, Yao Y, Kossenkov AV, Prasad R, Luan J, Davies JO, Hughes JR, Hardison RC, Blobel GA, Weiss MJ. Mol Cell. 2016 Apr 7;62(1):104-10. doi: 10.1016/j.molcel.2016.02.029. Epub 2016 Mar 31. PubMed
Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology Donors. Natsume T, Kiyomitsu T, Saga Y, Kanemaki MT. Cell Rep. 2016 Apr 5;15(1):210-8. doi: 10.1016/j.celrep.2016.03.001. Epub 2016 Mar 24. PubMed Associated Plasmids pMK277 (mClover-NeoR) pMK278 (mClover-Hygro) pMK279 (mClover-Bsr) pMK280 (mCherry2-NeoR) pMK281 (mCherry2-Hygro) More ...
Lentivirus pre-packed with Cas9 protein for safer gene editing. Choi JG, Dang Y, Abraham S, Ma H, Zhang J, Guo H, Cai Y, Mikkelsen JG, Wu H, Shankar P, Manjunath N. Gene Ther. 2016 Jul;23(7):627-33. doi: 10.1038/gt.2016.27. Epub 2016 Apr 7. PubMed
Generation of CRISPR/Cas9-mediated bicistronic knock-in Ins1-cre driver mice. Hasegawa Y, Hoshino Y, Ibrahim AE, Kato K, Daitoku Y, Tanimoto Y, Ikeda Y, Oishi H, Takahashi S, Yoshiki A, Yagami KI, Iseki H, Mizuno S, Sugiyama F. Exp Anim. 2016 Apr 7. PubMed
Cleavage by Caspase 8 and Mitochondrial Membrane Association Activate the BH3-only Protein Bid during TRAIL-induced Apoptosis. Huang K, Zhang J, O'Neill KL, Gurumurthy CB, Quadros RM, Tu Y, Luo X. J Biol Chem. 2016 May 27;291(22):11843-51. doi: 10.1074/jbc.M115.711051. Epub 2016 Apr 6. PubMed
The mitochondrial outer membrane protein MDI promotes local protein synthesis and mtDNA replication. Zhang Y, Chen Y, Gucek M, Xu H. EMBO J. 2016 May 17;35(10):1045-57. doi: 10.15252/embj.201592994. Epub 2016 Apr 6. PubMed
Inactivation of prosurvival Bcl-2 proteins activates Bax/Bak through the outer mitochondrial membrane. O'Neill KL, Huang K, Zhang J, Chen Y, Luo X. Genes Dev. 2016 Apr 15;30(8):973-88. doi: 10.1101/gad.276725.115. Epub 2016 Apr 7. PubMed
A Genome-wide CRISPR Screen Identifies CDC25A as a Determinant of Sensitivity to ATR Inhibitors. Ruiz S, Mayor-Ruiz C, Lafarga V, Murga M, Vega-Sendino M, Ortega S, Fernandez-Capetillo O. Mol Cell. 2016 Apr 21;62(2):307-313. doi: 10.1016/j.molcel.2016.03.006. Epub 2016 Apr 7. PubMed
Alternative splicing of MALT1 controls signalling and activation of CD4(+) T cells. Meininger I, Griesbach RA, Hu D, Gehring T, Seeholzer T, Bertossi A, Kranich J, Oeckinghaus A, Eitelhuber AC, Greczmiel U, Gewies A, Schmidt-Supprian M, Ruland J, Brocker T, Heissmeyer V, Heyd F, Krappmann D. Nat Commun. 2016 Apr 12;7:11292. doi: 10.1038/ncomms11292. PubMed
Effects of pharmacological and genetic disruption of CXCR4 chemokine receptor function in B-cell acute lymphoblastic leukaemia. Randhawa S, Cho BS, Ghosh D, Sivina M, Koehrer S, Muschen M, Peled A, Davis RE, Konopleva M, Burger JA. Br J Haematol. 2016 Apr 12. doi: 10.1111/bjh.14075. PubMed
Rapid and Efficient One-Step Metabolic Pathway Integration in E. coli. Bassalo MC, Garst AD, Halweg-Edwards AL, Grau WC, Domaille DW, Mutalik VK, Arkin AP, Gill RT. ACS Synth Biol. 2016 Apr 22. PubMed Associated Plasmids pSS9 SS9_RNA
EZH2-mediated repression of GSK-3beta and TP53 promotes Wnt/beta-catenin signaling-dependent cell expansion in cervical carcinoma. Chen Q, Zheng PS, Yang WT. Oncotarget. 2016 Jun 14;7(24):36115-36129. doi: 10.18632/oncotarget.8741. PubMed
A CRISPR Path to Engineering New Genetic Mouse Models for Cardiovascular Research. Miano JM, Zhu QM, Lowenstein CJ. Arterioscler Thromb Vasc Biol. 2016 Jun;36(6):1058-75. doi: 10.1161/ATVBAHA.116.304790. Epub 2016 Apr 21. PubMed
Generation of Human Embryonic Stem Cell Line Expressing zsGreen in Cholinergic Neurons Using CRISPR/Cas9 System. Zhou J, Wang C, Zhang K, Wang Y, Gong X, Wang Y, Li S, Luo Y. Neurochem Res. 2016 Apr 25. PubMed
Manipulating the Prion Protein Gene Sequence and Expression Levels with CRISPR/Cas9. Kaczmarczyk L, Mende Y, Zevnik B, Jackson WS. PLoS One. 2016 Apr 29;11(4):e0154604. doi: 10.1371/journal.pone.0154604. eCollection 2016. PubMed Associated Plasmids pX330_HR_Prnp_3 pX335_HR_Prnp_3 pX459_HR_Prnp_3 sgRNA(MS2)_Prnp_SAM1 sgRNA(MS2)_Prnp_SAM2
TPC2 controls pigmentation by regulating melanosome pH and size. Ambrosio AL, Boyle JA, Aradi AE, Christian KA, Di Pietro SM. Proc Natl Acad Sci U S A. 2016 May 17;113(20):5622-7. doi: 10.1073/pnas.1600108113. Epub 2016 May 2. PubMed Associated Plasmids MELOPS piRFP-N3-Tyrosinase
TRC8-dependent degradation of hepatitis C virus immature core protein regulates viral propagation and pathogenesis. Aizawa S, Okamoto T, Sugiyama Y, Kouwaki T, Ito A, Suzuki T, Ono C, Fukuhara T, Yamamoto M, Okochi M, Hiraga N, Imamura M, Chayama K, Suzuki R, Shoji I, Moriishi K, Moriya K, Koike K, Matsuura Y. Nat Commun. 2016 May 4;7:11379. doi: 10.1038/ncomms11379. PubMed
Lipoprotein Receptors Redundantly Participate in Entry of Hepatitis C Virus. Yamamoto S, Fukuhara T, Ono C, Uemura K, Kawachi Y, Shiokawa M, Mori H, Wada M, Shima R, Okamoto T, Hiraga N, Suzuki R, Chayama K, Wakita T, Matsuura Y. PLoS Pathog. 2016 May 6;12(5):e1005610. doi: 10.1371/journal.ppat.1005610. eCollection 2016 May. PubMed
Lactase nonpersistence is directed by DNA-variation-dependent epigenetic aging. Labrie V, Buske OJ, Oh E, Jeremian R, Ptak C, Gasiunas G, Maleckas A, Petereit R, Zvirbliene A, Adamonis K, Kriukiene E, Koncevicius K, Gordevicius J, Nair A, Zhang A, Ebrahimi S, Oh G, Siksnys V, Kupcinskas L, Brudno M, Petronis A. Nat Struct Mol Biol. 2016 Jun;23(6):566-73. doi: 10.1038/nsmb.3227. Epub 2016 May 9. PubMed
The SAGA Deubiquitination Module Promotes DNA Repair and Class Switch Recombination through ATM and DNAPK-Mediated gammaH2AX Formation. Ramachandran S, Haddad D, Li C, Le MX, Ling AK, So CC, Nepal RM, Gommerman JL, Yu K, Ketela T, Moffat J, Martin A. Cell Rep. 2016 May 17;15(7):1554-65. doi: 10.1016/j.celrep.2016.04.041. Epub 2016 May 5. PubMed
High-Throughput, High-Resolution Mapping of Protein Localization in Mammalian Brain by In Vivo Genome Editing. Mikuni T, Nishiyama J, Sun Y, Kamasawa N, Yasuda R. Cell. 2016 Jun 16;165(7):1803-17. doi: 10.1016/j.cell.2016.04.044. Epub 2016 May 12. PubMed
Mesp1 controls the speed, polarity, and directionality of cardiovascular progenitor migration. Chiapparo G, Lin X, Lescroart F, Chabab S, Paulissen C, Pitisci L, Bondue A, Blanpain C. J Cell Biol. 2016 May 23;213(4):463-77. doi: 10.1083/jcb.201505082. Epub 2016 May 16. PubMed
A nonsense mutation in the DNA repair factor Hebo causes mild bone marrow failure and microcephaly. Zhang S, Pondarre C, Pennarun G, Labussiere-Wallet H, Vera G, France B, Chansel M, Rouvet I, Revy P, Lopez B, Soulier J, Bertrand P, Callebaut I, de Villartay JP. J Exp Med. 2016 May 30;213(6):1011-28. doi: 10.1084/jem.20151183. Epub 2016 May 16. PubMed
Spermatogenic Cell-Specific Gene Mutation in Mice via CRISPR-Cas9. Bai M, Liang D, Wang Y, Li Q, Wu Y, Li J. J Genet Genomics. 2016 May 20;43(5):289-96. doi: 10.1016/j.jgg.2016.02.003. Epub 2016 Feb 26. PubMed
NSUN3 methylase initiates 5-formylcytidine biogenesis in human mitochondrial tRNA(Met). Nakano S, Suzuki T, Kawarada L, Iwata H, Asano K, Suzuki T. Nat Chem Biol. 2016 Jul;12(7):546-51. doi: 10.1038/nchembio.2099. Epub 2016 May 23. PubMed
Rapid and efficient analysis of gene function using CRISPR-Cas9 in Xenopus tropicalis founders. Shigeta M, Sakane Y, Iida M, Suzuki M, Kashiwagi K, Kashiwagi A, Fujii S, Yamamoto T, Suzuki KT. Genes Cells. 2016 Jul;21(7):755-71. doi: 10.1111/gtc.12379. Epub 2016 May 24. PubMed
Infantile Pain Episodes Associated with Novel Nav1.9 Mutations in Familial Episodic Pain Syndrome in Japanese Families. Okuda H, Noguchi A, Kobayashi H, Kondo D, Harada KH, Youssefian S, Shioi H, Kabata R, Domon Y, Kubota K, Kitano Y, Takayama Y, Hitomi T, Ohno K, Saito Y, Asano T, Tominaga M, Takahashi T, Koizumi A. PLoS One. 2016 May 25;11(5):e0154827. doi: 10.1371/journal.pone.0154827. eCollection 2016. PubMed
Generation of a Double KO Mouse by Simultaneous Targeting of the Neighboring Genes Tmem176a and Tmem176b Using CRISPR/Cas9: Key Steps from Design to Genotyping. Lemoine A, Chauveau-Le Friec G, Langa F, Louvet C. J Genet Genomics. 2016 May 20;43(5):329-40. doi: 10.1016/j.jgg.2016.04.004. Epub 2016 Apr 22. PubMed
Functional validation of tensin2 SH2-PTB domain by CRISPR/Cas9-mediated genome editing. Marusugi K, Nakano K, Sasaki H, Kimura J, Yanobu-Takanashi R, Okamura T, Sasaki N. J Vet Med Sci. 2016 May 30. PubMed
Hyperlipidemia and hepatitis in liver-specific CREB3L3 knockout mice generated using a one-step CRISPR/Cas9 system. Nakagawa Y, Oikawa F, Mizuno S, Ohno H, Yagishita Y, Satoh A, Osaki Y, Takei K, Kikuchi T, Han SI, Matsuzaka T, Iwasaki H, Kobayashi K, Yatoh S, Yahagi N, Isaka M, Suzuki H, Sone H, Takahashi S, Yamada N, Shimano H. Sci Rep. 2016 Jun 13;6:27857. doi: 10.1038/srep27857. PubMed
Unconventional secretion of misfolded proteins promotes adaptation to proteasome dysfunction in mammalian cells. Lee JG, Takahama S, Zhang G, Tomarev SI, Ye Y. Nat Cell Biol. 2016 Jul;18(7):765-76. doi: 10.1038/ncb3372. Epub 2016 Jun 13. PubMed Associated Plasmids pRK-flag-USP19 1-1290 pRK-flag-USP19 494-1290 pRK-flag-USP19 1-493 pRK-flag-USP19 C506S USP19 sgRNA1 More ...
Constitutive Activation of PINK1 Protein Leads to Proteasome-mediated and Non-apoptotic Cell Death Independently of Mitochondrial Autophagy. Akabane S, Matsuzaki K, Yamashita S, Arai K, Okatsu K, Kanki T, Matsuda N, Oka T. J Biol Chem. 2016 Jul 29;291(31):16162-74. doi: 10.1074/jbc.M116.714923. Epub 2016 Jun 14. PubMed
Bax/Bak activation in the absence of Bid, Bim, Puma, and p53. Zhang J, Huang K, O'Neill KL, Pang X, Luo X. Cell Death Dis. 2016 Jun 16;7:e2266. doi: 10.1038/cddis.2016.167. PubMed
Inactivation of the CRL4-CDT2-SET8/p21 ubiquitylation and degradation axis underlies the therapeutic efficacy of pevonedistat in melanoma. Benamar M, Guessous F, Du K, Corbett P, Obeid J, Gioeli D, Slingluff CL Jr, Abbas T. EBioMedicine. 2016 Jun 16. pii: S2352-3964(16)30279-1. doi: 10.1016/j.ebiom.2016.06.023. PubMed
S100A4 accelerates the proliferation and invasion of endometrioid carcinoma and is associated with the "MELF" pattern. Tahara S, Nojima S, Ohshima K, Hori Y, Kurashige M, Wada N, Ikeda J, Morii E. Cancer Sci. 2016 Sep;107(9):1345-52. doi: 10.1111/cas.12999. Epub 2016 Aug 12. PubMed
Loss of function mutation in LOX causes thoracic aortic aneurysm and dissection in humans. Lee VS, Halabi CM, Hoffman EP, Carmichael N, Leshchiner I, Lian CG, Bierhals AJ, Vuzman D, Mecham RP, Frank NY, Stitziel NO. Proc Natl Acad Sci U S A. 2016 Aug 2;113(31):8759-64. doi: 10.1073/pnas.1601442113. Epub 2016 Jul 18. PubMed
BRCA1185delAG tumors may acquire therapy resistance through expression of RING-less BRCA1. Drost R, Dhillon KK, van der Gulden H, van der Heijden I, Brandsma I, Cruz C, Chondronasiou D, Castroviejo-Bermejo M, Boon U, Schut E, van der Burg E, Wientjens E, Pieterse M, Klijn C, Klarenbeek S, Loayza-Puch F, Elkon R, van Deemter L, Rottenberg S, van de Ven M, Dekkers DH, Demmers JA, van Gent DC, Agami R, Balmana J, Serra V, Taniguchi T, Bouwman P, Jonkers J. J Clin Invest. 2016 Aug 1;126(8):2903-18. doi: 10.1172/JCI70196. Epub 2016 Jul 25. PubMed
Co-incident insertion enables high efficiency genome engineering in mouse embryonic stem cells. Shy BR, MacDougall MS, Clarke R, Merrill BJ. Nucleic Acids Res. 2016 Aug 2. pii: gkw685. PubMed
Investigation of the functional role of human Interleukin-8 gene haplotypes by CRISPR/Cas9 mediated genome editing. Benakanakere MR, Finoti LS, Tanaka U, Grant GR, Scarel-Caminaga RM, Kinane DF. Sci Rep. 2016 Aug 8;6:31180. doi: 10.1038/srep31180. PubMed
Live Cell Imaging Reveals the Dynamics of Telomerase Recruitment to Telomeres. Schmidt JC, Zaug AJ, Cech TR. Cell. 2016 Aug 25;166(5):1188-1197.e9. doi: 10.1016/j.cell.2016.07.033. Epub 2016 Aug 11. PubMed Associated Plasmids FLAG-LoxP-PURO-LoxP-HaloTERT WT HR Donor HA-mEOS3.2-TRF2 HR Donor
Non-homologous DNA increases gene disruption efficiency by altering DNA repair outcomes. Richardson CD, Ray GJ, Bray NL, Corn JE. Nat Commun. 2016 Aug 17;7:12463. doi: 10.1038/ncomms12463. PubMed
CRISPR/Cas9 mediated genome editing in ES cells and its application for chimeric analysis in mice. Oji A, Noda T, Fujihara Y, Miyata H, Kim YJ, Muto M, Nozawa K, Matsumura T, Isotani A, Ikawa M. Sci Rep. 2016 Aug 17;6:31666. doi: 10.1038/srep31666. PubMed
Comprehensive Protocols for CRISPR/Cas9-based Gene Editing in Human Pluripotent Stem Cells. Santos DP, Kiskinis E, Eggan K, Merkle FT. Curr Protoc Stem Cell Biol. 2016 Aug 17;38:5B.6.1-5B.6.60. doi: 10.1002/cpsc.15. PubMed
The type 2 diabetes presumed causal variant within TCF7L2 resides in an element that controls the expression of ACSL5. Xia Q, Chesi A, Manduchi E, Johnston BT, Lu S, Leonard ME, Parlin UW, Rappaport EF, Huang P, Wells AD, Blobel GA, Johnson ME, Grant SF. Diabetologia. 2016 Nov;59(11):2360-8. doi: 10.1007/s00125-016-4077-2. Epub 2016 Aug 18. PubMed
53BP1 Integrates DNA Repair and p53-Dependent Cell Fate Decisions via Distinct Mechanisms. Cuella-Martin R, Oliveira C, Lockstone HE, Snellenberg S, Grolmusova N, Chapman JR. Mol Cell. 2016 Oct 6;64(1):51-64. doi: 10.1016/j.molcel.2016.08.002. Epub 2016 Aug 18. PubMed
Impaired striatal dopamine release in homozygous Vps35 D620N knock-in mice. Ishizu N, Yui D, Hebisawa A, Aizawa H, Cui W, Fujita Y, Hashimoto K, Ajioka I, Mizusawa H, Yokota T, Watase K. Hum Mol Genet. 2016 Aug 25. pii: ddw279. PubMed
Covalent targeting of remote cysteine residues to develop CDK12 and CDK13 inhibitors. Zhang T, Kwiatkowski N, Olson CM, Dixon-Clarke SE, Abraham BJ, Greifenberg AK, Ficarro SB, Elkins JM, Liang Y, Hannett NM, Manz T, Hao M, Bartkowiak B, Greenleaf AL, Marto JA, Geyer M, Bullock AN, Young RA, Gray NS. Nat Chem Biol. 2016 Oct;12(10):876-84. doi: 10.1038/nchembio.2166. Epub 2016 Aug 29. PubMed
Generation of DeltaF508-CFTR T84 cell lines by CRISPR/Cas9-mediated genome editing. Chung WY, Song M, Park J, Namkung W, Lee J, Kim H, Lee MG, Kim JY. Biotechnol Lett. 2016 Dec;38(12):2023-2034. Epub 2016 Aug 29. PubMed
Reprogramming metabolic pathways in vivo with CRISPR/Cas9 genome editing to treat hereditary tyrosinaemia. Pankowicz FP, Barzi M, Legras X, Hubert L, Mi T, Tomolonis JA, Ravishankar M, Sun Q, Yang D, Borowiak M, Sumazin P, Elsea SH, Bissig-Choisat B, Bissig KD. Nat Commun. 2016 Aug 30;7:12642. doi: 10.1038/ncomms12642. PubMed
Genome editing with CRISPR/Cas9 in postnatal mice corrects PRKAG2 cardiac syndrome. Xie C, Zhang YP, Song L, Luo J, Qi W, Hu J, Lu D, Yang Z, Zhang J, Xiao J, Zhou B, Du JL, Jing N, Liu Y, Wang Y, Li BL, Song BL, Yan Y. Cell Res. 2016 Oct;26(10):1099-1111. doi: 10.1038/cr.2016.101. Epub 2016 Aug 30. PubMed
Long noncoding RNA #32 contributes to antiviral responses by controlling interferon-stimulated gene expression. Nishitsuji H, Ujino S, Yoshio S, Sugiyama M, Mizokami M, Kanto T, Shimotohno K. Proc Natl Acad Sci U S A. 2016 Sep 13;113(37):10388-93. doi: 10.1073/pnas.1525022113. Epub 2016 Aug 31. PubMed
CRISPR/Cas9 Technology Targeting Fas Gene Protects Mice From Concanavalin-A Induced Fulminant Hepatic Failure. Liang WC, Liang PP, Wong CW, Ng TB, Huang JJ, Zhang JF, Waye MM, Fu WM. J Cell Biochem. 2017 Mar;118(3):530-536. doi: 10.1002/jcb.25722. Epub 2016 Oct 12. PubMed
Epithelial Expression of Human ABO Blood Group Genes Is Dependent upon a Downstream Regulatory Element Functioning through an Epithelial Cell-specific Transcription Factor, Elf5. Sano R, Nakajima T, Takahashi Y, Kubo R, Kobayashi M, Takahashi K, Takeshita H, Ogasawara K, Kominato Y. J Biol Chem. 2016 Oct 21;291(43):22594-22606. Epub 2016 Sep 1. PubMed
The Development of a Viral Mediated CRISPR/Cas9 System with Doxycycline Dependent gRNA Expression for Inducible In vitro and In vivo Genome Editing. de Solis CA, Ho A, Holehonnur R, Ploski JE. Front Mol Neurosci. 2016 Aug 18;9:70. doi: 10.3389/fnmol.2016.00070. eCollection 2016. PubMed Associated Plasmids pJEP15-AAV-H1/TO-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA pJEP14-AAV-H1/TO-sgRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA pJEP13-AAV-H1/TO-L-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA pJEP12-AAV-H1/TO-L-sgRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA pJEP11-AAV-U6/TO-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA More ...
Sendai virus, an RNA virus with no risk of genomic integration, delivers CRISPR/Cas9 for efficient gene editing. Park A, Hong P, Won ST, Thibault PA, Vigant F, Oguntuyo KY, Taft JD, Lee B. Mol Ther Methods Clin Dev. 2016 Aug 24;3:16057. doi: 10.1038/mtm.2016.57. eCollection 2016. PubMed
Analyses of point mutation repair and allelic heterogeneity generated by CRISPR/Cas9 and single-stranded DNA oligonucleotides. Bialk P, Sansbury B, Rivera-Torres N, Bloh K, Man D, Kmiec EB. Sci Rep. 2016 Sep 9;6:32681. doi: 10.1038/srep32681. PubMed
Efficient production of biallelic GGTA1 knockout pigs by cytoplasmic microinjection of CRISPR/Cas9 into zygotes. Petersen B, Frenzel A, Lucas-Hahn A, Herrmann D, Hassel P, Klein S, Ziegler M, Hadeler KG, Niemann H. Xenotransplantation. 2016 Sep;23(5):338-46. doi: 10.1111/xen.12258. Epub 2016 Sep 9. PubMed
Perichromosomal protein Ki67 supports mitotic chromosome architecture. Takagi M, Natsume T, Kanemaki MT, Imamoto N. Genes Cells. 2016 Oct;21(10):1113-1124. doi: 10.1111/gtc.12420. Epub 2016 Sep 9. PubMed
A G-Rich Motif in the lncRNA Braveheart Interacts with a Zinc-Finger Transcription Factor to Specify the Cardiovascular Lineage. Xue Z, Hennelly S, Doyle B, Gulati AA, Novikova IV, Sanbonmatsu KY, Boyer LA. Mol Cell. 2016 Oct 6;64(1):37-50. doi: 10.1016/j.molcel.2016.08.010. Epub 2016 Sep 8. PubMed
PtdIns(4,5)P2 signaling regulates ATG14 and autophagy. Tan X, Thapa N, Liao Y, Choi S, Anderson RA. Proc Natl Acad Sci U S A. 2016 Sep 27;113(39):10896-901. doi: 10.1073/pnas.1523145113. Epub 2016 Sep 12. PubMed
CRISPR/Cas9-mediated mutation revealed cytoplasmic tail is dispensable for IZUMO1 function and male fertility. Young SA, Miyata H, Satouh Y, Muto M, Larsen MR, Aitken RJ, Baker MA, Ikawa M. Reproduction. 2016 Dec;152(6):665-672. Epub 2016 Sep 13. PubMed
Cpf1 nucleases demonstrate robust activity to induce DNA modification by exploiting homology directed repair pathways in mammalian cells. Toth E, Weinhardt N, Bencsura P, Huszar K, Kulcsar PI, Talas A, Fodor E, Welker E. Biol Direct. 2016 Sep 14;11:46. doi: 10.1186/s13062-016-0147-0. PubMed Associated Plasmids pTE4396 pTE4398 WN10151 pTE4254 pKS7107 More ...
The scaffolding function of the RLTPR protein explains its essential role for CD28 co-stimulation in mouse and human T cells. Roncagalli R, Cucchetti M, Jarmuzynski N, Gregoire C, Bergot E, Audebert S, Baudelet E, Menoita MG, Joachim A, Durand S, Suchanek M, Fiore F, Zhang L, Liang Y, Camoin L, Malissen M, Malissen B. J Exp Med. 2016 Oct 17;213(11):2437-2457. Epub 2016 Sep 19. PubMed
Tetherin is an exosomal tether. Edgar JR, Manna PT, Nishimura S, Banting G, Robinson MS. Elife. 2016 Sep 22;5. pii: e17180. doi: 10.7554/eLife.17180. PubMed
Mitochondrial Protein Synthesis Adapts to Influx of Nuclear-Encoded Protein. Richter-Dennerlein R, Oeljeklaus S, Lorenzi I, Ronsor C, Bareth B, Schendzielorz AB, Wang C, Warscheid B, Rehling P, Dennerlein S. Cell. 2016 Oct 6;167(2):471-483.e10. doi: 10.1016/j.cell.2016.09.003. Epub 2016 Sep 29. PubMed
Upregulation of ANGPTL6 in mouse keratinocytes enhances susceptibility to psoriasis. Tanigawa H, Miyata K, Tian Z, Aoi J, Kadomatsu T, Fukushima S, Ogata A, Takeda N, Zhao J, Zhu S, Terada K, Endo M, Morinaga J, Sugizaki T, Sato M, Morioka MS, Manabe I, Mashimo Y, Hata A, Taketomi Y, Yamamoto K, Murakami M, Araki K, Jinnin M, Ihn H, Oike Y. Sci Rep. 2016 Oct 4;6:34690. doi: 10.1038/srep34690. PubMed
MyoD Regulates Skeletal Muscle Oxidative Metabolism Cooperatively with Alternative NF-kappaB. Shintaku J, Peterson JM, Talbert EE, Gu JM, Ladner KJ, Williams DR, Mousavi K, Wang R, Sartorelli V, Guttridge DC. Cell Rep. 2016 Oct 4;17(2):514-526. doi: 10.1016/j.celrep.2016.09.010. PubMed
Evolution of Hoxa11 regulation in vertebrates is linked to the pentadactyl state. Kherdjemil Y, Lalonde RL, Sheth R, Dumouchel A, de Martino G, Pineault KM, Wellik DM, Stadler HS, Akimenko MA, Kmita M. Nature. 2016 Nov 3;539(7627):89-92. doi: 10.1038/nature19813. Epub 2016 Oct 5. PubMed
Foot-and-Mouth Disease Virus Viroporin 2B Antagonizes RIG-I-Mediated Antiviral Effects by Inhibition of Its Protein Expression. Zhu Z, Wang G, Yang F, Cao W, Mao R, Du X, Zhang X, Li C, Li D, Zhang K, Shu H, Liu X, Zheng H. J Virol. 2016 Nov 28;90(24):11106-11121. Print 2016 Dec 15. PubMed
Design and Validation of CRISPR/Cas9 Systems for Targeted Gene Modification in Induced Pluripotent Stem Cells. Lee CM, Zhu H, Davis TH, Deshmukh H, Bao G. Methods Mol Biol. 2017;1498:3-21. PubMed
CRISPR/Cas9 mediated knockout of rb1 and rbl1 leads to rapid and penetrant retinoblastoma development in Xenopus tropicalis. Naert T, Colpaert R, Van Nieuwenhuysen T, Dimitrakopoulou D, Leoen J, Haustraete J, Boel A, Steyaert W, Lepez T, Deforce D, Willaert A, Creytens D, Vleminckx K. Sci Rep. 2016 Oct 14;6:35264. doi: 10.1038/srep35264. PubMed
Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Luo W, Mizuno H, Iwata R, Nakazawa S, Yasuda K, Itohara S, Iwasato T. Sci Rep. 2016 Oct 24;6:35747. doi: 10.1038/srep35747. PubMed Associated Plasmids pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova) pK036.TRE-Flpe-WPRE (Supernova) pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE (Supernova) pK037.CAG-FRT-stop-FRT-RFP-ires-tTA-WPRE (Supernova) pK168.AAV-EF1α-DIO-tTA-P2A-RFP-WPRE (Supernova) More ...
The Impact of Chromatin Dynamics on Cas9-Mediated Genome Editing in Human Cells. Daer RM, Cutts JP, Brafman DA, Haynes KA. ACS Synth Biol. 2017 Mar 17;6(3):428-438. doi: 10.1021/acssynbio.5b00299. Epub 2016 Nov 17. PubMed
C14ORF39/SIX6OS1 is a constituent of the synaptonemal complex and is essential for mouse fertility. Gomez-H L, Felipe-Medina N, Sanchez-Martin M, Davies OR, Ramos I, Garcia-Tunon I, de Rooij DG, Dereli I, Toth A, Barbero JL, Benavente R, Llano E, Pendas AM. Nat Commun. 2016 Oct 31;7:13298. doi: 10.1038/ncomms13298. PubMed
DNA methylation-independent removable insulator controls chromatin remodeling at the HOXA locus via retinoic acid signaling. Ishihara K, Nakamoto M, Nakao M. Hum Mol Genet. 2016 Oct 24. pii: ddw354. doi: 10.1093/hmg/ddw354. PubMed
CABYR is essential for fibrous sheath integrity and progressive motility in mouse spermatozoa. Young SA, Miyata H, Satouh Y, Aitken RJ, Baker MA, Ikawa M. J Cell Sci. 2016 Dec 1;129(23):4379-4387. Epub 2016 Oct 19. PubMed
Structural and functional consequences of a disease mutation in the telomere protein TPP1. Bisht K, Smith EM, Tesmer VM, Nandakumar J. Proc Natl Acad Sci U S A. 2016 Nov 15;113(46):13021-13026. Epub 2016 Nov 2. PubMed
Calreticulin mutant mice develop essential thrombocythemia that is ameliorated by the JAK inhibitor ruxolitinib. Shide K, Kameda T, Yamaji T, Sekine M, Inada N, Kamiunten A, Akizuki K, Nakamura K, Hidaka T, Kubuki Y, Shimoda H, Kitanaka A, Honda A, Sawaguchi A, Abe H, Miike T, Iwakiri H, Tahara Y, Sueta M, Hasuike S, Yamamoto S, Nagata K, Shimoda K. Leukemia. 2016 Nov 29. doi: 10.1038/leu.2016.308. PubMed
RIPK1 counteracts ZBP1-mediated necroptosis to inhibit inflammation. Lin J, Kumari S, Kim C, Van TM, Wachsmuth L, Polykratis A, Pasparakis M. Nature. 2016 Dec 1;540(7631):124-128. doi: 10.1038/nature20558. Epub 2016 Nov 7. PubMed
Re-visiting the Protamine-2 locus: deletion, but not haploinsufficiency, renders male mice infertile. Schneider S, Balbach M, Jan F Jikeli, Fietz D, Nettersheim D, Jostes S, Schmidt R, Kressin M, Bergmann M, Wachten D, Steger K, Schorle H. Sci Rep. 2016 Nov 11;6:36764. doi: 10.1038/srep36764. PubMed
Epigenetic upregulation of ARL4C, due to DNA hypomethylation in the 3'-untranslated region, promotes tumorigenesis of lung squamous cell carcinoma. Fujii S, Shinjo K, Matsumoto S, Harada T, Nojima S, Sato S, Usami Y, Toyosawa S, Morii E, Kondo Y, Kikuchi A. Oncotarget. 2016 Dec 6;7(49):81571-81587. doi: 10.18632/oncotarget.13147. PubMed
Phospho-ubiquitin-PARK2 complex as a marker for mitophagy defects. Callegari S, Oeljeklaus S, Warscheid B, Dennerlein S, Thumm M, Rehling P, Dudek J. Autophagy. 2017 Jan 2;13(1):201-211. doi: 10.1080/15548627.2016.1254852. Epub 2016 Nov 15. PubMed
Kin17 facilitates multiple double-strand break repair pathways that govern B cell class switching. Le MX, Haddad D, Ling AK, Li C, So CC, Chopra A, Hu R, Angulo JF, Moffat J, Martin A. Sci Rep. 2016 Nov 17;6:37215. doi: 10.1038/srep37215. PubMed
pKAMA-ITACHI vectors for highly efficient CRISPR/Cas9-mediated gene knockout in Arabidopsis thaliana. Tsutsui H, Higashiyama T. Plant Cell Physiol. 2016 Nov 17. pii: pcw191. PubMed Associated Plasmids pKIR1.1 pKI1.1 pKI1.1R pKIR1.0
Long-Range Control of Renin Gene Expression in Tsukuba Hypertensive Mice. Ushiki A, Matsuzaki H, Ishida J, Fukamizu A, Tanimoto K. PLoS One. 2016 Nov 18;11(11):e0166974. doi: 10.1371/journal.pone.0166974. eCollection 2016. PubMed
Mouse Tmem135 mutation reveals a mechanism involving mitochondrial dynamics that leads to age-dependent retinal pathologies. Lee WH, Higuchi H, Ikeda S, Macke EL, Takimoto T, Pattnaik BR, Liu C, Chu LF, Siepka SM, Krentz KJ, Rubinstein CD, Kalejta RF, Thomson JA, Mullins RF, Takahashi JS, Pinto LH, Ikeda A. Elife. 2016 Nov 15;5. pii: e19264. doi: 10.7554/eLife.19264. PubMed
PRDM14 Drives OCT3/4 Recruitment via Active Demethylation in the Transition from Primed to Naive Pluripotency. Okashita N, Suwa Y, Nishimura O, Sakashita N, Kadota M, Nagamatsu G, Kawaguchi M, Kashida H, Nakajima A, Tachibana M, Seki Y. Stem Cell Reports. 2016 Dec 13;7(6):1072-1086. doi: 10.1016/j.stemcr.2016.10.007. Epub 2016 Nov 17. PubMed
The HECT domain ubiquitin ligase HUWE1 targets unassembled soluble proteins for degradation. Xu Y, Anderson DE, Ye Y. Cell Discov. 2016 Nov 8;2:16040. eCollection 2016. PubMed Associated Plasmids pVENUS-N1-Ubl4A pcDNA5/FRT/TO-FNTA-FLAG pVENUS-N1-FNTA pcDNA5/FRT/TO-FNTB-HA pEGFP-C1-GFP-SF3B6-FLAG More ...
Loss of maternal ANNEXIN A10 via a 34-kb deleted-type copy number variation is associated with embryonic mortality in Japanese Black cattle. Sasaki S, Ibi T, Akiyama T, Fukushima M, Sugimoto Y. BMC Genomics. 2016 Nov 24;17(1):968. PubMed
Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ. Aida T, Nakade S, Sakuma T, Izu Y, Oishi A, Mochida K, Ishikubo H, Usami T, Aizawa H, Yamamoto T, Tanaka K. BMC Genomics. 2016 Nov 28;17(1):979. PubMed
Efficient Generation of Orthologous Point Mutations in Pigs via CRISPR-assisted ssODN-mediated Homology-directed Repair. Wang K, Tang X, Liu Y, Xie Z, Zou X, Li M, Yuan H, Ouyang H, Jiao H, Pang D. Mol Ther Nucleic Acids. 2016 Nov 29;5(11):e396. doi: 10.1038/mtna.2016.101. PubMed
Deficiency of a Retinal Dystrophy Protein, Acyl-CoA Binding Domain-containing 5 (ACBD5), Impairs Peroxisomal beta-Oxidation of Very-long-chain Fatty Acids. Yagita Y, Shinohara K, Abe Y, Nakagawa K, Al-Owain M, Alkuraya FS, Fujiki Y. J Biol Chem. 2017 Jan 13;292(2):691-705. doi: 10.1074/jbc.M116.760090. Epub 2016 Nov 29. PubMed
An Ultraconserved Element (UCE) controls homeostatic splicing of ARGLU1 mRNA. Pirnie SP, Osman A, Zhu Y, Carmichael GG. Nucleic Acids Res. 2016 Nov 29. pii: gkw1140. PubMed
Mitochondrial division occurs concurrently with autophagosome formation but independently of Drp1 during mitophagy. Yamashita SI, Jin X, Furukawa K, Hamasaki M, Nezu A, Otera H, Saigusa T, Yoshimori T, Sakai Y, Mihara K, Kanki T. J Cell Biol. 2016 Dec 5;215(5):649-665. Epub 2016 Nov 30. PubMed
A novel approach to assess the ubiquitin-fold modifier 1-system in cells. Ishimura R, Obata M, Kageyama S, Daniel J, Tanaka K, Komatsu M. FEBS Lett. 2017 Jan;591(1):196-204. doi: 10.1002/1873-3468.12518. Epub 2016 Dec 20. PubMed
Genome-Wide CRISPR Screen Identifies Regulators of Mitogen-Activated Protein Kinase as Suppressors of Liver Tumors in Mice. Song CQ, Li Y, Mou H, Moore J, Park A, Pomyen Y, Hough S, Kennedy Z, Fischer A, Yin H, Anderson DG, Conte D Jr, Zender L, Wang XW, Thorgeirsson S, Weng Z, Xue W. Gastroenterology. 2017 Apr;152(5):1161-1173.e1. doi: 10.1053/j.gastro.2016.12.002. Epub 2016 Dec 10. PubMed Associated Plasmids lentiCRISPRv2.sgNf1.1 pX330.sgNf1.4 lentiCRISPRv2.sgNf1.4
Loss of glyoxalase 1 induces compensatory mechanism to achieve dicarbonyl detoxification in mammalian Schwann cells. Morgenstern J, Fleming T, Schumacher D, Eckstein V, Freichel M, Herzig S, Nawroth P. J Biol Chem. 2016 Dec 12. pii: jbc.M116.760132. PubMed
Histone acetylation of glucose-induced thioredoxin-interacting protein gene expression in pancreatic islets. Bompada P, Atac D, Luan C, Andersson R, Omella JD, Laakso EO, Wright J, Groop L, De Marinis Y. Int J Biochem Cell Biol. 2016 Oct 29. pii: S1357-2725(16)30329-6. doi: 10.1016/j.biocel.2016.10.022. PubMed
A Critical Role for the Type I Interferon Receptor in Virus-Induced Autoimmune Diabetes in Rats. Qaisar N, Lin S, Ryan G, Yang C, Oikemus SR, Brodsky MH, Bortell R, Mordes JP, Wang JP. Diabetes. 2017 Jan;66(1):145-157. doi: 10.2337/db16-0462. Epub 2016 Oct 7. PubMed
CRISPR/Cas9-mediated integration enables TAG-eCLIP of endogenously tagged RNA binding proteins. Van Nostrand EL, Gelboin-Burkhart C, Wang R, Pratt GA, Blue SM, Yeo GW. Methods. 2016 Dec 18. pii: S1046-2023(16)30481-9. doi: 10.1016/j.ymeth.2016.12.007. PubMed
Deletion of the GAA repeats from the human frataxin gene using the CRISPR-Cas9 system in YG8R-derived cells and mouse models of Friedreich ataxia. Ouellet DL, Cherif K, Rousseau J, Tremblay JP. Gene Ther. 2017 May;24(5):265-274. doi: 10.1038/gt.2016.89. Epub 2016 Dec 26. PubMed
Using the CRISPR/Cas9 system to understand neuropeptide biology and regulation. Hay EA, Knowles C, Kolb A, MacKenzie A. Neuropeptides. 2016 Dec 3. pii: S0143-4179(16)30169-X. doi: 10.1016/j.npep.2016.11.010. PubMed
Inhibition of CRISPR-Cas9 with Bacteriophage Proteins. Rauch BJ, Silvis MR, Hultquist JF, Waters CS, McGregor MJ, Krogan NJ, Bondy-Denomy J. Cell. 2016 Dec 22. pii: S0092-8674(16)31683-X. doi: 10.1016/j.cell.2016.12.009. PubMed Associated Plasmids pRAU168 pRAU171 pCSW21 pCSW24 pCSW65 More ...
Normal Levels of Sox9 Expression in the Developing Mouse Testis Depend on the TES/TESCO Enhancer, but This Does Not Act Alone. Gonen N, Quinn A, O'Neill HC, Koopman P, Lovell-Badge R. PLoS Genet. 2017 Jan 3;13(1):e1006520. doi: 10.1371/journal.pgen.1006520. eCollection 2017 Jan. PubMed
Essential Role for Polycomb Group Protein Pcgf6 in Embryonic Stem Cell Maintenance and a Noncanonical Polycomb Repressive Complex 1 (PRC1) Integrity. Zhao W, Tong H, Huang Y, Yan Y, Teng H, Xia Y, Jiang Q, Qin J. J Biol Chem. 2017 Feb 17;292(7):2773-2784. doi: 10.1074/jbc.M116.763961. Epub 2017 Jan 3. PubMed
Insertional Mutagenesis by CRISPR/Cas9 Ribonucleoprotein Gene Editing in Cells Targeted for Point Mutation Repair Directed by Short Single-Stranded DNA Oligonucleotides. Rivera-Torres N, Banas K, Bialk P, Bloh KM, Kmiec EB. PLoS One. 2017 Jan 4;12(1):e0169350. doi: 10.1371/journal.pone.0169350. eCollection 2017. PubMed
Contribution of canonical nonhomologous end joining to chromosomal rearrangements is enhanced by ATM kinase deficiency. Bhargava R, Carson CR, Lee G, Stark JM. Proc Natl Acad Sci U S A. 2017 Jan 5. pii: 201612204. doi: 10.1073/pnas.1612204114. PubMed
Functional Study of the Vitamin K Cycle Enzymes in Live Cells. Tie JK, Stafford DW. Methods Enzymol. 2017;584:349-394. doi: 10.1016/bs.mie.2016.10.015. Epub 2016 Nov 22. PubMed
Initiation of Quality Control during Poly(A) Translation Requires Site-Specific Ribosome Ubiquitination. Juszkiewicz S, Hegde RS. Mol Cell. 2017 Feb 16;65(4):743-750.e4. doi: 10.1016/j.molcel.2016.11.039. Epub 2017 Jan 5. PubMed Associated Plasmids pmGFP-P2A-K0-P2A-RFP pmGFP-P2A-K(AAA)12-P2A-RFP pmGFP-P2A-K(AAA)20-P2A-RFP pmGFP-P2A-K(AAG)20-P2A-RFP pcDNA3.1-ZNF598-TEV-3xFLAG
Temporally-Distinct PD-L1 Expression by Tumor and Host Cells Contributes to Immune Escape. Noguchi T, Ward JP, Gubin MM, Arthur CD, Lee SH, Hundal J, Selby M, Graziano RF, Mardis ER, Korman AJ, Schreiber RD. Cancer Immunol Res. 2017 Jan 10. pii: canimm.0391.2016. doi: 10.1158/2326-6066.CIR-16-0391. PubMed
Generation of a Stable Transgenic Swine Model Expressing a Porcine Histone 2B-eGFP Fusion Protein for Cell Tracking and Chromosome Dynamics Studies. Sper RB, Koh S, Zhang X, Simpson S, Collins B, Sommer J, Petters RM, Caballero I, Platt JL, Piedrahita JA. PLoS One. 2017 Jan 12;12(1):e0169242. doi: 10.1371/journal.pone.0169242. eCollection 2017. PubMed
Maternal Supply of Cas9 to Zygotes Facilitates the Efficient Generation of Site-Specific Mutant Mouse Models. Cebrian-Serrano A, Zha S, Hanssen L, Biggs D, Preece C, Davies B. PLoS One. 2017 Jan 12;12(1):e0169887. doi: 10.1371/journal.pone.0169887. eCollection 2017. PubMed
Noncanonical function of DGCR8 controls mESC exit from pluripotency. Cirera-Salinas D, Yu J, Bodak M, Ngondo RP, Herbert KM, Ciaudo C. J Cell Biol. 2017 Jan 18. pii: jcb.201606073. doi: 10.1083/jcb.201606073. PubMed Associated Plasmids pX330-sgRNA_Dgcr8_1 pX330-sgRNA_Dgcr8_2 pX330-sgRNA_Dgcr8_3 pX330-sgRNA_Dgcr8_4 pX330-sgRNA_Drosha_1 More ...
Loss of Myh14 Increases Susceptibility to Noise-Induced Hearing Loss in CBA/CaJ Mice. Fu X, Zhang L, Jin Y, Sun X, Zhang A, Wen Z, Zhou Y, Xia M, Gao J. Neural Plast. 2016;2016:6720420. doi: 10.1155/2016/6720420. Epub 2016 Dec 22. PubMed
SOX10-Nano-Lantern Reporter Human iPS Cells; A Versatile Tool for Neural Crest Research. Horikiri T, Ohi H, Shibata M, Ikeya M, Ueno M, Sotozono C, Kinoshita S, Sato T. PLoS One. 2017 Jan 20;12(1):e0170342. doi: 10.1371/journal.pone.0170342. eCollection 2017. PubMed
The application of CRISPR technology to high content screening in primary neurons. Callif BL, Maunze B, Krueger NL, Simpson MT, Blackmore MG. Mol Cell Neurosci. 2017 Apr;80:170-179. doi: 10.1016/j.mcn.2017.01.003. Epub 2017 Jan 19. PubMed
An Argonaute phosphorylation cycle promotes microRNA-mediated silencing. Golden RJ, Chen B, Li T, Braun J, Manjunath H, Chen X, Wu J, Schmid V, Chang TC, Kopp F, Ramirez-Martinez A, Tagliabracci VS, Chen ZJ, Xie Y, Mendell JT. Nature. 2017 Feb 9;542(7640):197-202. doi: 10.1038/nature21025. Epub 2017 Jan 23. PubMed Associated Plasmids MSCV-EGFP MSCV-EGFP-Bulged-miR-19 LcV2-Hygro pLJM1-FH-AGO2-WT pLJM1-FH-AGO2-5xA More ...
Targeted CRISPR disruption reveals a role for RNase MRP RNA in human preribosomal RNA processing. Goldfarb KC, Cech TR. Genes Dev. 2017 Jan 1;31(1):59-71. doi: 10.1101/gad.286963.116. Epub 2017 Jan 23. PubMed Associated Plasmids px330-MRPsg1 px330-MRPsg2 px330-MRPsg3 px330-MRPsg4 psglessClonePX More ...
Human BCDIN3D monomethylates cytoplasmic histidine transfer RNA. Martinez A, Yamashita S, Nagaike T, Sakaguchi Y, Suzuki T, Tomita K. Nucleic Acids Res. 2017 May 19;45(9):5423-5436. doi: 10.1093/nar/gkx051. PubMed
PLCE1 Promotes Esophageal Cancer Cell Progression by Maintaining the Transcriptional Activity of Snail. Zhai S, Liu C, Zhang L, Zhu J, Guo J, Zhang J, Chen Z, Zhou W, Chang T, Xu S, Qi Y, Zhuang T, Yu N, Wang W, Wang H, Yu S, Li X. Neoplasia. 2017 Mar;19(3):154-164. doi: 10.1016/j.neo.2016.12.007. Epub 2017 Jan 29. PubMed
Genome Editing Reveals Glioblastoma Addiction to MicroRNA-10b. El Fatimy R, Subramanian S, Uhlmann EJ, Krichevsky AM. Mol Ther. 2017 Feb 1;25(2):368-378. doi: 10.1016/j.ymthe.2016.11.004. PubMed
Polycomb Repressive Complex 1 Generates Discrete Compacted Domains that Change during Differentiation. Kundu S, Ji F, Sunwoo H, Jain G, Lee JT, Sadreyev RI, Dekker J, Kingston RE. Mol Cell. 2017 Feb 2;65(3):432-446.e5. doi: 10.1016/j.molcel.2017.01.009. PubMed
An interaction domain in human SAMD9 is essential for myxoma virus host-range determinant M062 antagonism of host anti-viral function. Nounamo B, Li Y, O'Byrne P, Kearney AM, Khan A, Liu J. Virology. 2017 Mar;503:94-102. doi: 10.1016/j.virol.2017.01.004. Epub 2017 Jan 31. PubMed
An Efficient Electroporation Protocol for the Genetic Modification of Mammalian Cells. Chicaybam L, Barcelos C, Peixoto B, Carneiro M, Limia CG, Redondo P, Lira C, Paraguassu-Braga F, Vasconcelos ZF, Barros L, Bonamino MH. Front Bioeng Biotechnol. 2017 Jan 23;4:99. doi: 10.3389/fbioe.2016.00099. eCollection 2016. PubMed Associated Plasmids pT3-Neo pT3-Neo-EF1a-GFP
Impaired striatal dopamine release in homozygous Vps35 D620N knock-in mice. Ishizu N, Yui D, Hebisawa A, Aizawa H, Cui W, Fujita Y, Hashimoto K, Ajioka I, Mizusawa H, Yokota T, Watase K. Hum Mol Genet. 2016 Oct 15;25(20):4507-4517. doi: 10.1093/hmg/ddw279. PubMed
Efficient genome editing in the mouse brain by local delivery of engineered Cas9 ribonucleoprotein complexes. Staahl BT, Benekareddy M, Coulon-Bainier C, Banfal AA, Floor SN, Sabo JK, Urnes C, Munares GA, Ghosh A, Doudna JA. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3806. PubMed Associated Plasmids pMJ915v2+Nterm Spe1 +Cterm Age1 site 1xNLS-pMJ915v2 2xNLS-pMJ915v2 4xNLS-pMJ915v2 pMJ915v2 + sfGFP More ...
MicroRNA 301A Promotes Intestinal Inflammation and Colitis-Associated Cancer Development by Inhibiting BTG1. He C, Yu T, Shi Y, Ma C, Yang W, Fang L, Sun M, Wu W, Xiao F, Guo F, Chen M, Yang H, Qian J, Cong Y, Liu Z. Gastroenterology. 2017 May;152(6):1434-1448.e15. doi: 10.1053/j.gastro.2017.01.049. Epub 2017 Feb 11. PubMed
CBX3 promotes colon cancer cell proliferation by CDK6 kinase-independent function during cell cycle. Fan Y, Li H, Liang X, Xiang Z. Oncotarget. 2017 Mar 21;8(12):19934-19946. doi: 10.18632/oncotarget.15253. PubMed
CRISPR/Cas9-mediated genome editing in wild-derived mice: generation of tamed wild-derived strains by mutation of the a (nonagouti) gene. Hirose M, Hasegawa A, Mochida K, Matoba S, Hatanaka Y, Inoue K, Goto T, Kaneda H, Yamada I, Furuse T, Abe K, Uenoyama Y, Tsukamura H, Wakana S, Honda A, Ogura A. Sci Rep. 2017 Feb 14;7:42476. doi: 10.1038/srep42476. PubMed
Biallelic insertion of a transcriptional terminator via the CRISPR/Cas9 system efficiently silences expression of protein-coding and non-coding RNA genes. Liu Y, Han X, Yuan J, Geng T, Chen S, Hu X, Cui IH, Cui H. J Biol Chem. 2017 Apr 7;292(14):5624-5633. doi: 10.1074/jbc.M116.769034. Epub 2017 Feb 14. PubMed
Distinct C-mannosylation of netrin receptor thrombospondin type 1 repeats by mammalian DPY19L1 and DPY19L3. Shcherbakova A, Tiemann B, Buettner FF, Bakker H. Proc Natl Acad Sci U S A. 2017 Mar 7;114(10):2574-2579. doi: 10.1073/pnas.1613165114. Epub 2017 Feb 15. PubMed
Luman contributes to brefeldin A-induced prion protein gene expression by interacting with the ERSE26 element. Dery MA, LeBlanc AC. Sci Rep. 2017 Feb 13;7:42285. doi: 10.1038/srep42285. PubMed
A novel LSD1 inhibitor NCD38 ameliorates MDS-related leukemia with complex karyotype by attenuating leukemia programs via activating super-enhancers. Sugino N, Kawahara M, Tatsumi G, Kanai A, Matsui H, Yamamoto R, Nagai Y, Fujii S, Shimazu Y, Hishizawa M, Inaba T, Andoh A, Suzuki T, Takaori-Kondo A. Leukemia. 2017 Nov;31(11):2303-2314. doi: 10.1038/leu.2017.59. Epub 2017 Feb 17. PubMed
Conformational Profiling of the AT1 Angiotensin II Receptor Reflects Biased Agonism, G Protein Coupling, and Cellular Context. Devost D, Sleno R, Petrin D, Zhang A, Shinjo Y, Okde R, Aoki J, Inoue A, Hebert TE. J Biol Chem. 2017 Mar 31;292(13):5443-5456. doi: 10.1074/jbc.M116.763854. Epub 2017 Feb 17. PubMed
Somatic Mutations in TSC1 and TSC2 Cause Focal Cortical Dysplasia. Lim JS, Gopalappa R, Kim SH, Ramakrishna S, Lee M, Kim WI, Kim J, Park SM, Lee J, Oh JH, Kim HD, Park CH, Lee JS, Kim S, Kim DS, Han JM, Kang HC, Kim HH, Lee JH. Am J Hum Genet. 2017 Mar 2;100(3):454-472. doi: 10.1016/j.ajhg.2017.01.030. Epub 2017 Feb 16. PubMed
Targeted RP9 ablation and mutagenesis in mouse photoreceptor cells by CRISPR-Cas9. Lv JN, Zhou GH, Chen X, Chen H, Wu KC, Xiang L, Lei XL, Zhang X, Wu RH, Jin ZB. Sci Rep. 2017 Feb 20;7:43062. doi: 10.1038/srep43062. PubMed
Efficient Precision Genome Editing in iPSCs via Genetic Co-targeting with Selection. Mitzelfelt KA, McDermott-Roe C, Grzybowski MN, Marquez M, Kuo CT, Riedel M, Lai S, Choi MJ, Kolander KD, Helbling D, Dimmock DP, Battle MA, Jou CJ, Tristani-Firouzi M, Verbsky JW, Benjamin IJ, Geurts AM. Stem Cell Reports. 2017 Mar 14;8(3):491-499. doi: 10.1016/j.stemcr.2017.01.021. Epub 2017 Feb 24. PubMed
Systems-based analysis of RIG-I-dependent signalling identifies KHSRP as an inhibitor of RIG-I receptor activation. Soonthornvacharin S, Rodriguez-Frandsen A, Zhou Y, Galvez F, Huffmaster NJ, Tripathi S, Balasubramaniam VR, Inoue A, de Castro E, Moulton H, Stein DA, Sanchez-Aparicio MT, De Jesus PD, Nguyen Q, Konig R, Krogan NJ, Garcia-Sastre A, Yoh SM, Chanda SK. Nat Microbiol. 2017 Mar 1;2:17022. doi: 10.1038/nmicrobiol.2017.22. PubMed
Inhibition of CRISPR/Cas9-Mediated Genome Engineering by a Type I Interferon-Induced Reduction in Guide RNA Expression. Machitani M, Sakurai F, Wakabayashi K, Nakatani K, Takayama K, Tachibana M, Mizuguchi H. Biol Pharm Bull. 2017;40(3):272-277. doi: 10.1248/bpb.b16-00700. PubMed
Rational design of inducible CRISPR guide RNAs for de novo assembly of transcriptional programs. Ferry QR, Lyutova R, Fulga TA. Nat Commun. 2017 Mar 3;8:14633. doi: 10.1038/ncomms14633. PubMed
Substrate specificity of TOR complex 2 is determined by a ubiquitin-fold domain of the Sin1 subunit. Tatebe H, Murayama S, Yonekura T, Hatano T, Richter D, Furuya T, Kataoka S, Furuita K, Kojima C, Shiozaki K. Elife. 2017 Mar 7;6. pii: e19594. doi: 10.7554/eLife.19594. PubMed
The Product of the Herpes Simplex Virus 2 UL16 Gene Is Critical for the Egress of Capsids from the Nuclei of Infected Cells. Gao J, Hay TJM, Banfield BW. J Virol. 2017 Apr 28;91(10). pii: e00350-17. doi: 10.1128/JVI.00350-17. Print 2017 May 15. PubMed
CRISPR-Cas9 system-driven site-specific selection pressure on Herpes simplex virus genomes. Li Z, Bi Y, Xiao H, Sun L, Ren Y, Li Y, Chen C, Cun W. Virus Res. 2017 Mar 6. pii: S0168-1702(16)30694-3. doi: 10.1016/j.virusres.2017.03.010. PubMed
PRC2 Facilitates the Regulatory Topology Required for Poised Enhancer Function during Pluripotent Stem Cell Differentiation. Cruz-Molina S, Respuela P, Tebartz C, Kolovos P, Nikolic M, Fueyo R, van Ijcken WFJ, Grosveld F, Frommolt P, Bazzi H, Rada-Iglesias A. Cell Stem Cell. 2017 May 4;20(5):689-705.e9. doi: 10.1016/j.stem.2017.02.004. Epub 2017 Mar 9. PubMed
Identification of common non-coding variants at 1p22 that are functional for non-syndromic orofacial clefting. Liu H, Leslie EJ, Carlson JC, Beaty TH, Marazita ML, Lidral AC, Cornell RA. Nat Commun. 2017 Mar 13;8:14759. doi: 10.1038/ncomms14759. PubMed
Loss of LMOD1 impairs smooth muscle cytocontractility and causes megacystis microcolon intestinal hypoperistalsis syndrome in humans and mice. Halim D, Wilson MP, Oliver D, Brosens E, Verheij JB, Han Y, Nanda V, Lyu Q, Doukas M, Stoop H, Brouwer RW, van IJcken WF, Slivano OJ, Burns AJ, Christie CK, de Mesy Bentley KL, Brooks AS, Tibboel D, Xu S, Jin ZG, Djuwantono T, Yan W, Alves MM, Hofstra RM, Miano JM. Proc Natl Acad Sci U S A. 2017 Mar 28;114(13):E2739-E2747. doi: 10.1073/pnas.1620507114. Epub 2017 Mar 14. PubMed
Pseudomonas aeruginosa infection liberates transmissible, cytotoxic prion amyloids. Balczon R, Morrow KA, Zhou C, Edmonds B, Alexeyev M, Pittet JF, Wagener BM, Moser SA, Leavesley S, Zha X, Frank DW, Stevens T. FASEB J. 2017 Jul;31(7):2785-2796. doi: 10.1096/fj.201601042RR. Epub 2017 Mar 17. PubMed
DNA damage during S-phase mediates the proliferation-quiescence decision in the subsequent G1 via p21 expression. Barr AR, Cooper S, Heldt FS, Butera F, Stoy H, Mansfeld J, Novak B, Bakal C. Nat Commun. 2017 Mar 20;8:14728. doi: 10.1038/ncomms14728. PubMed
Chd7 is indispensable for mammalian brain development through activation of a neuronal differentiation programme. Feng W, Kawauchi D, Korkel-Qu H, Deng H, Serger E, Sieber L, Lieberman JA, Jimeno-Gonzalez S, Lambo S, Hanna BS, Harim Y, Jansen M, Neuerburg A, Friesen O, Zuckermann M, Rajendran V, Gronych J, Ayrault O, Korshunov A, Jones DT, Kool M, Northcott PA, Lichter P, Cortes-Ledesma F, Pfister SM, Liu HK. Nat Commun. 2017 Mar 20;8:14758. doi: 10.1038/ncomms14758. PubMed
TRH Action Is Impaired in Pituitaries of Male IGSF1-Deficient Mice. Turgeon MO, Silander TL, Doycheva D, Liao XH, Rigden M, Ongaro L, Zhou X, Joustra SD, Wit JM, Wade MG, Heuer H, Refetoff S, Bernard DJ. Endocrinology. 2017 Apr 1;158(4):815-830. doi: 10.1210/en.2016-1788. PubMed
Increased Expression of Laminin Subunit Alpha 1 Chain by dCas9-VP160. Perrin A, Rousseau J, Tremblay JP. Mol Ther Nucleic Acids. 2017 Mar 17;6:68-79. doi: 10.1016/j.omtn.2016.11.004. Epub 2016 Dec 10. PubMed
Sensing of Bacterial Cyclic Dinucleotides by the Oxidoreductase RECON Promotes NF-kappaB Activation and Shapes a Proinflammatory Antibacterial State. McFarland AP, Luo S, Ahmed-Qadri F, Zuck M, Thayer EF, Goo YA, Hybiske K, Tong L, Woodward JJ. Immunity. 2017 Mar 21;46(3):433-445. doi: 10.1016/j.immuni.2017.02.014. PubMed
Targeted gene knock-in by homology-directed genome editing using Cas9 ribonucleoprotein and AAV donor delivery. Gaj T, Staahl BT, Rodrigues GM, Limsirichai P, Ekman FK, Doudna JA, Schaffer DV. Nucleic Acids Res. 2017 Mar 2. doi: 10.1093/nar/gkx154. PubMed
Circ-ZNF609 Is a Circular RNA that Can Be Translated and Functions in Myogenesis. Legnini I, Di Timoteo G, Rossi F, Morlando M, Briganti F, Sthandier O, Fatica A, Santini T, Andronache A, Wade M, Laneve P, Rajewsky N, Bozzoni I. Mol Cell. 2017 Apr 6;66(1):22-37.e9. doi: 10.1016/j.molcel.2017.02.017. Epub 2017 Mar 23. PubMed
Visualization and targeting of LGR5(+) human colon cancer stem cells. Shimokawa M, Ohta Y, Nishikori S, Matano M, Takano A, Fujii M, Date S, Sugimoto S, Kanai T, Sato T. Nature. 2017 May 11;545(7653):187-192. doi: 10.1038/nature22081. Epub 2017 Mar 29. PubMed Associated Plasmids HR180-LGR5-GFP HR180-LGR5-CreER HR180-LGR5-iCT
BATF2 inhibits immunopathological Th17 responses by suppressing Il23a expression during Trypanosoma cruzi infection. Kitada S, Kayama H, Okuzaki D, Koga R, Kobayashi M, Arima Y, Kumanogoh A, Murakami M, Ikawa M, Takeda K. J Exp Med. 2017 May 1;214(5):1313-1331. doi: 10.1084/jem.20161076. Epub 2017 Mar 29. PubMed
Sialylation Is Dispensable for Early Murine Embryonic Development in Vitro. Abeln M, Borst KM, Cajic S, Thiesler H, Kats E, Albers I, Kuhn M, Kaever V, Rapp E, Munster-Kuhnel A, Weinhold B. Chembiochem. 2017 Jul 4;18(13):1305-1316. doi: 10.1002/cbic.201700083. Epub 2017 May 11. PubMed
Derivation of Pluripotent Stem Cells with In Vivo Embryonic and Extraembryonic Potency. Yang Y, Liu B, Xu J, Wang J, Wu J, Shi C, Xu Y, Dong J, Wang C, Lai W, Zhu J, Xiong L, Zhu D, Li X, Yang W, Yamauchi T, Sugawara A, Li Z, Sun F, Li X, Li C, He A, Du Y, Wang T, Zhao C, Li H, Chi X, Zhang H, Liu Y, Li C, Duo S, Yin M, Shen H, Belmonte JCI, Deng H. Cell. 2017 Apr 6;169(2):243-257.e25. doi: 10.1016/j.cell.2017.02.005. PubMed
The BET Protein BRD2 Cooperates with CTCF to Enforce Transcriptional and Architectural Boundaries. Hsu SC, Gilgenast TG, Bartman CR, Edwards CR, Stonestrom AJ, Huang P, Emerson DJ, Evans P, Werner MT, Keller CA, Giardine B, Hardison RC, Raj A, Phillips-Cremins JE, Blobel GA. Mol Cell. 2017 Apr 6;66(1):102-116.e7. doi: 10.1016/j.molcel.2017.02.027. PubMed
Loss of Polycomb Group Protein Pcgf1 Severely Compromises Proper Differentiation of Embryonic Stem Cells. Yan Y, Zhao W, Huang Y, Tong H, Xia Y, Jiang Q, Qin J. Sci Rep. 2017 Apr 10;7:46276. doi: 10.1038/srep46276. PubMed
Association of C-Type Lectin Mincle with FcepsilonRIbetagamma Subunits Leads to Functional Activation of RBL-2H3 Cells through Syk. Honjoh C, Chihara K, Yoshiki H, Yamauchi S, Takeuchi K, Kato Y, Hida Y, Ishizuka T, Sada K. Sci Rep. 2017 Apr 10;7:46064. doi: 10.1038/srep46064. PubMed
CRISPR/Cas9-Mediated Gene Knockout in the Mouse Brain Using In Utero Electroporation. Shinmyo Y, Kawasaki H. Curr Protoc Neurosci. 2017 Apr 10;79:3.32.1-3.32.11. doi: 10.1002/cpns.26. PubMed
Unusual semi-extractability as a hallmark of nuclear body-associated architectural noncoding RNAs. Chujo T, Yamazaki T, Kawaguchi T, Kurosaka S, Takumi T, Nakagawa S, Hirose T. EMBO J. 2017 May 15;36(10):1447-1462. doi: 10.15252/embj.201695848. Epub 2017 Apr 12. PubMed
Genome-Wide Screening Uncovers the Significance of N-Sulfation of Heparan Sulfate as a Host Cell Factor for Chikungunya Virus Infection. Tanaka A, Tumkosit U, Nakamura S, Motooka D, Kishishita N, Priengprom T, Sa-Ngasang A, Kinoshita T, Takeda N, Maeda Y. J Virol. 2017 Jun 9;91(13). pii: e00432-17. doi: 10.1128/JVI.00432-17. Print 2017 Jul 1. PubMed
Foot-and-mouth disease virus infection inhibits LGP2 protein expression to exaggerate inflammatory response and promote viral replication. Zhu Z, Li C, Du X, Wang G, Cao W, Yang F, Feng H, Zhang X, Shi Z, Liu H, Tian H, Li D, Zhang K, Liu X, Zheng H. Cell Death Dis. 2017 Apr 13;8(4):e2747. doi: 10.1038/cddis.2017.170. PubMed
Identification of a novel functional JAK1 S646P mutation in acute lymphoblastic leukemia. Li Q, Li B, Hu L, Ning H, Jiang M, Wang D, Liu T, Zhang B, Chen H. Oncotarget. 2017 May 23;8(21):34687-34697. doi: 10.18632/oncotarget.16670. PubMed
A tiling-deletion-based genetic screen for cis-regulatory element identification in mammalian cells. Diao Y, Fang R, Li B, Meng Z, Yu J, Qiu Y, Lin KC, Huang H, Liu T, Marina RJ, Jung I, Shen Y, Guan KL, Ren B. Nat Methods. 2017 Jun;14(6):629-635. doi: 10.1038/nmeth.4264. Epub 2017 Apr 17. PubMed
An efficient method to enrich for knock-out and knock-in cellular clones using the CRISPR/Cas9 system. Niccheri F, Pecori R, Conticello SG. Cell Mol Life Sci. 2017 Apr 18. doi: 10.1007/s00018-017-2524-y. PubMed Associated Plasmids pBS SK mCherryROSAbsr pBS SK mCherryROSAegfp pBSR (mCherry-surrogateTarget-bsr)
Minus end-directed kinesin-14 KIFC1 regulates the positioning and architecture of the Golgi apparatus. She ZY, Pan MY, Tan FQ, Yang WX. Oncotarget. 2017 May 30;8(22):36469-36483. doi: 10.18632/oncotarget.16863. PubMed
A Compendium of RNA-Binding Proteins that Regulate MicroRNA Biogenesis. Treiber T, Treiber N, Plessmann U, Harlander S, Daiss JL, Eichner N, Lehmann G, Schall K, Urlaub H, Meister G. Mol Cell. 2017 Apr 20;66(2):270-284.e13. doi: 10.1016/j.molcel.2017.03.014. PubMed
Identification of subepithelial mesenchymal cells that induce IgA and diversify gut microbiota. Nagashima K, Sawa S, Nitta T, Tsutsumi M, Okamura T, Penninger JM, Nakashima T, Takayanagi H. Nat Immunol. 2017 Jun;18(6):675-682. doi: 10.1038/ni.3732. Epub 2017 Apr 24. PubMed
SWELL1 is a regulator of adipocyte size, insulin signalling and glucose homeostasis. Zhang Y, Xie L, Gunasekar SK, Tong D, Mishra A, Gibson WJ, Wang C, Fidler T, Marthaler B, Klingelhutz A, Abel ED, Samuel I, Smith JK, Cao L, Sah R. Nat Cell Biol. 2017 May;19(5):504-517. doi: 10.1038/ncb3514. Epub 2017 Apr 24. PubMed
PARP3 is a promoter of chromosomal rearrangements and limits G4 DNA. Day TA, Layer JV, Cleary JP, Guha S, Stevenson KE, Tivey T, Kim S, Schinzel AC, Izzo F, Doench J, Root DE, Hahn WC, Price BD, Weinstock DM. Nat Commun. 2017 Apr 27;8:15110. doi: 10.1038/ncomms15110. PubMed
Construction of a highly efficient CRISPR/Cas9-mediated duck enteritis virus-based vaccine against H5N1 avian influenza virus and duck Tembusu virus infection. Zou Z, Huang K, Wei Y, Chen H, Liu Z, Jin M. Sci Rep. 2017 May 3;7(1):1478. doi: 10.1038/s41598-017-01554-1. PubMed
ALKBH1 is an RNA dioxygenase responsible for cytoplasmic and mitochondrial tRNA modifications. Kawarada L, Suzuki T, Ohira T, Hirata S, Miyauchi K, Suzuki T. Nucleic Acids Res. 2017 May 2. doi: 10.1093/nar/gkx354. PubMed
The Cohesin Release Factor WAPL Restricts Chromatin Loop Extension. Haarhuis JHI, van der Weide RH, Blomen VA, Yanez-Cuna JO, Amendola M, van Ruiten MS, Krijger PHL, Teunissen H, Medema RH, van Steensel B, Brummelkamp TR, de Wit E, Rowland BD. Cell. 2017 May 4;169(4):693-707.e14. doi: 10.1016/j.cell.2017.04.013. PubMed
A lentivirus-free inducible CRISPR-Cas9 system for efficient targeting of human genes. Bisht K, Grill S, Graniel J, Nandakumar J. Anal Biochem. 2017 Aug 1;530:40-49. doi: 10.1016/j.ab.2017.05.001. Epub 2017 May 4. PubMed
Acute inactivation of the replicative helicase in human cells triggers MCM8-9-dependent DNA synthesis. Natsume T, Nishimura K, Minocherhomji S, Bhowmick R, Hickson ID, Kanemaki MT. Genes Dev. 2017 Apr 15;31(8):816-829. doi: 10.1101/gad.297663.117. Epub 2017 May 9. PubMed
Proline metabolism supports metastasis formation and could be inhibited to selectively target metastasizing cancer cells. Elia I, Broekaert D, Christen S, Boon R, Radaelli E, Orth MF, Verfaillie C, Grunewald TGP, Fendt SM. Nat Commun. 2017 May 11;8:15267. doi: 10.1038/ncomms15267. PubMed
Characterization of miR-122-independent propagation of HCV. Ono C, Fukuhara T, Motooka D, Nakamura S, Okuzaki D, Yamamoto S, Tamura T, Mori H, Sato A, Uemura K, Fauzyah Y, Kurihara T, Suda T, Nishio A, Hmwe SS, Okamoto T, Tatsumi T, Takehara T, Chayama K, Wakita T, Koike K, Matsuura Y. PLoS Pathog. 2017 May 11;13(5):e1006374. doi: 10.1371/journal.ppat.1006374. eCollection 2017 May. PubMed
The murine catecholamine methyltransferase mTOMT is essential for mechanotransduction by cochlear hair cells. Cunningham CL, Wu Z, Jafari A, Zhao B, Schrode K, Harkins-Perry S, Lauer A, Muller U. Elife. 2017 May 15;6. pii: e24318. doi: 10.7554/eLife.24318. PubMed
MK2 Phosphorylates RIPK1 to Prevent TNF-Induced Cell Death. Jaco I, Annibaldi A, Lalaoui N, Wilson R, Tenev T, Laurien L, Kim C, Jamal K, Wicky John S, Liccardi G, Chau D, Murphy JM, Brumatti G, Feltham R, Pasparakis M, Silke J, Meier P. Mol Cell. 2017 Jun 1;66(5):698-710.e5. doi: 10.1016/j.molcel.2017.05.003. Epub 2017 May 11. PubMed
TRAF3 enhances TCR signaling by regulating the inhibitors Csk and PTPN22. Wallis AM, Wallace EC, Hostager BS, Yi Z, Houtman JCD, Bishop GA. Sci Rep. 2017 May 18;7(1):2081. doi: 10.1038/s41598-017-02280-4. PubMed
Homology-mediated end joining-based targeted integration using CRISPR/Cas9. Yao X, Wang X, Hu X, Liu Z, Liu J, Zhou H, Shen X, Wei Y, Huang Z, Ying W, Wang Y, Nie YH, Zhang CC, Li S, Cheng L, Wang Q, Wu Y, Huang P, Sun Q, Shi L, Yang H. Cell Res. 2017 May 19. doi: 10.1038/cr.2017.76. PubMed Associated Plasmids AAV_Efs_hSpCas9_NLS_FLAG-SV40 AAV_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA AAV_Actb HR donor_U6_sgRNA_EF1a_GFP_polyA AAV_Actb NHEJ donor_U6_sgRNA_EF1a_GFP_polyA AAV_Actb MMEJ donor_U6_sgRNA_EF1a_GFP_polyA More ...
Targeted Degradation of CTCF Decouples Local Insulation of Chromosome Domains from Genomic Compartmentalization. Nora EP, Goloborodko A, Valton AL, Gibcus JH, Uebersohn A, Abdennur N, Dekker J, Mirny LA, Bruneau BG. Cell. 2017 May 18;169(5):930-944.e22. doi: 10.1016/j.cell.2017.05.004. PubMed Associated Plasmids pEN84 - CTCF-AID[71-114]-eGFP-FRT-Puro-FRT targeting construct pX335-EN475 pX335-EN477 pEN113 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-NeoR-bpA-Frt-Rosa26 pX330-EN479 More ...
CRISPR/Cas9 - Mediated Precise Targeted Integration In Vivo Using a Double Cut Donor with Short Homology Arms. Yao X, Wang X, Liu J, Hu X, Shi L, Shen X, Ying W, Sun X, Wang X, Huang P, Yang H. EBioMedicine. 2017 Jun;20:19-26. doi: 10.1016/j.ebiom.2017.05.015. Epub 2017 May 11. PubMed
Hit and go CAS9 delivered through a lentiviral based self-limiting circuit. Petris G, Casini A, Montagna C, Lorenzin F, Prandi D, Romanel A, Zasso J, Conti L, Demichelis F, Cereseto A. Nat Commun. 2017 May 22;8:15334. doi: 10.1038/ncomms15334. PubMed
CD7-edited T cells expressing a CD7-specific CAR for the therapy of T-cell malignancies. Gomes-Silva D, Srinivasan M, Sharma S, Lee CM, Wagner DL, Davis TH, Rouce RH, Bao G, Brenner MK, Mamonkin M. Blood. 2017 Jul 20;130(3):285-296. doi: 10.1182/blood-2017-01-761320. Epub 2017 May 24. PubMed
Extracellular Interactions between Hepatitis C Virus and Secreted Apolipoprotein E. Li Z, Li Y, Bi Y, Zhang H, Yao Y, Li Q, Cun W, Dong S. J Virol. 2017 Jul 12;91(15). pii: e02227-16. doi: 10.1128/JVI.02227-16. Print 2017 Aug 1. PubMed
Efficient generation of targeted and controlled mutational events in porcine cells using nuclease-directed homologous recombination. Butler JR, Santos RMN, Martens GR, Ladowski JM, Wang ZY, Li P, Tector M, Tector AJ. J Surg Res. 2017 May 15;212:238-245. doi: 10.1016/j.jss.2017.01.025. Epub 2017 Jan 29. PubMed
Necroptosis and ferroptosis are alternative cell death pathways that operate in acute kidney failure. Muller T, Dewitz C, Schmitz J, Schroder AS, Brasen JH, Stockwell BR, Murphy JM, Kunzendorf U, Krautwald S. Cell Mol Life Sci. 2017 May 27. doi: 10.1007/s00018-017-2547-4. PubMed
Generation of carbamoyl phosphate synthetase 1 reporter cell lines for the assessment of ammonia metabolism. Wang Y, Chang L, Zhai J, Wu Q, Wang D, Wang Y. J Cell Mol Med. 2017 May 30. doi: 10.1111/jcmm.13225. PubMed
Argininosuccinate synthase 1 is an intrinsic Akt repressor transactivated by p53. Miyamoto T, Lo PHY, Saichi N, Ueda K, Hirata M, Tanikawa C, Matsuda K. Sci Adv. 2017 May 19;3(5):e1603204. doi: 10.1126/sciadv.1603204. eCollection 2017 May. PubMed
Systematic discovery of mutation-specific synthetic lethals by mining pan-cancer human primary tumor data. Sinha S, Thomas D, Chan S, Gao Y, Brunen D, Torabi D, Reinisch A, Hernandez D, Chan A, Rankin EB, Bernards R, Majeti R, Dill DL. Nat Commun. 2017 May 31;8:15580. doi: 10.1038/ncomms15580. PubMed
Genetic wiring maps of single-cell protein states reveal an off-switch for GPCR signalling. Brockmann M, Blomen VA, Nieuwenhuis J, Stickel E, Raaben M, Bleijerveld OB, Altelaar AFM, Jae LT, Brummelkamp TR. Nature. 2017 Jun 8;546(7657):307-311. doi: 10.1038/nature22376. Epub 2017 May 31. PubMed
Proof-of-Concept Gene Editing for the Murine Model of Inducible Arginase-1 Deficiency. Sin YY, Price PR, Ballantyne LL, Funk CD. Sci Rep. 2017 May 31;7(1):2585. doi: 10.1038/s41598-017-02927-2. PubMed
A CRISPR Cas9 high-throughput genome editing toolkit for kinetoplastids. Beneke T, Madden R, Makin L, Valli J, Sunter J, Gluenz E. R Soc Open Sci. 2017 May 3;4(5):170095. doi: 10.1098/rsos.170095. eCollection 2017 May. PubMed
MyoD- and FoxO3-mediated hotspot interaction orchestrates super-enhancer activity during myogenic differentiation. Peng XL, So KK, He L, Zhao Y, Zhou J, Li Y, Yao M, Xu B, Zhang S, Yao H, Hu P, Sun H, Wang H. Nucleic Acids Res. 2017 Jun 1. doi: 10.1093/nar/gkx488. PubMed
The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity. Simsek D, Tiu GC, Flynn RA, Byeon GW, Leppek K, Xu AF, Chang HY, Barna M. Cell. 2017 Jun 1;169(6):1051-1065.e18. doi: 10.1016/j.cell.2017.05.022. PubMed
CRISPR-STOP: gene silencing through base-editing-induced nonsense mutations. Kuscu C, Parlak M, Tufan T, Yang J, Szlachta K, Wei X, Mammadov R, Adli M. Nat Methods. 2017 Jul;14(7):710-712. doi: 10.1038/nmeth.4327. Epub 2017 Jun 5. PubMed
Precise and Efficient In-Frame Integration of an Exogenous GFP Tag in Aspergillus fumigatus by a CRISPR System. Zhang C, Lu L. Methods Mol Biol. 2017;1625:249-258. doi: 10.1007/978-1-4939-7104-6_17. PubMed
Detection of genome-edited mutant clones by a simple competition-based PCR method. Harayama T, Riezman H. PLoS One. 2017 Jun 6;12(6):e0179165. doi: 10.1371/journal.pone.0179165. eCollection 2017. PubMed
Structure of the Rpn13-Rpn2 complex provides insights for Rpn13 and Uch37 as anticancer targets. Lu X, Nowicka U, Sridharan V, Liu F, Randles L, Hymel D, Dyba M, Tarasov SG, Tarasova NI, Zhao XZ, Hamazaki J, Murata S, Burke TR Jr, Walters KJ. Nat Commun. 2017 Jun 9;8:15540. doi: 10.1038/ncomms15540. PubMed
Centromeres are maintained by fastening CENP-A to DNA and directing an arginine anchor-dependent nucleosome transition. Guo LY, Allu PK, Zandarashvili L, McKinley KL, Sekulic N, Dawicki-McKenna JM, Fachinetti D, Logsdon GA, Jamiolkowski RM, Cleveland DW, Cheeseman IM, Black BE. Nat Commun. 2017 Jun 9;8:15775. doi: 10.1038/ncomms15775. PubMed
Regulated Erlin-dependent release of the B12 transmembrane J-protein promotes ER membrane penetration of a non-enveloped virus. Inoue T, Tsai B. PLoS Pathog. 2017 Jun 14;13(6):e1006439. doi: 10.1371/journal.ppat.1006439. eCollection 2017 Jun. PubMed
CRISPR/Cas9-mediated genome editing induces exon skipping by alternative splicing or exon deletion. Mou H, Smith JL, Peng L, Yin H, Moore J, Zhang XO, Song CQ, Sheel A, Wu Q, Ozata DM, Li Y, Anderson DG, Emerson CP, Sontheimer EJ, Moore MJ, Weng Z, Xue W. Genome Biol. 2017 Jun 14;18(1):108. doi: 10.1186/s13059-017-1237-8. PubMed
Sexually dimorphic distribution of Prokr2 neurons revealed by the Prokr2-Cre mouse model. Mohsen Z, Sim H, Garcia-Galiano D, Han X, Bellefontaine N, Saunders TL, Elias CF. Brain Struct Funct. 2017 Jun 14. doi: 10.1007/s00429-017-1456-5. PubMed
Critical role of CREBH-mediated induction of TGF-beta2 by HCV infection in fibrogenic responses in hepatic stellate cells. Chida T, Ito M, Nakashima K, Kanegae Y, Aoshima T, Takabayashi S, Kawata K, Nakagawa Y, Yamamoto M, Shimano H, Matsuura T, Kobayashi Y, Suda T, Suzuki T. Hepatology. 2017 Jun 16. doi: 10.1002/hep.29319. PubMed
CRISPR/Cas9-loxP-Mediated Gene Editing as a Novel Site-Specific Genetic Manipulation Tool. Yang F, Liu C, Chen D, Tu M, Xie H, Sun H, Ge X, Tang L, Li J, Zheng J, Song Z, Qu J, Gu F. Mol Ther Nucleic Acids. 2017 Jun 16;7:378-386. doi: 10.1016/j.omtn.2017.04.018. Epub 2017 Apr 25. PubMed
Heterogeneous Ribosomes Preferentially Translate Distinct Subpools of mRNAs Genome-wide. Shi Z, Fujii K, Kovary KM, Genuth NR, Rost HL, Teruel MN, Barna M. Mol Cell. 2017 Jul 6;67(1):71-83.e7. doi: 10.1016/j.molcel.2017.05.021. Epub 2017 Jun 15. PubMed
Genetic evidence that beta-arrestins are dispensable for the initiation of beta2-adrenergic receptor signaling to ERK. O'Hayre M, Eichel K, Avino S, Zhao X, Steffen DJ, Feng X, Kawakami K, Aoki J, Messer K, Sunahara R, Inoue A, von Zastrow M, Gutkind JS. Sci Signal. 2017 Jun 20;10(484). pii: 10/484/eaal3395. doi: 10.1126/scisignal.aal3395. PubMed
Genome Editing in Mouse Zygotes and Embryonic Stem Cells by Introducing SgRNA/Cas9 Expressing Plasmids. Noda T, Oji A, Ikawa M. Methods Mol Biol. 2017;1630:67-80. doi: 10.1007/978-1-4939-7128-2_6. PubMed
Host-derived apolipoproteins play comparable roles with viral secretory proteins Erns and NS1 in the infectious particle formation of Flaviviridae. Fukuhara T, Tamura T, Ono C, Shiokawa M, Mori H, Uemura K, Yamamoto S, Kurihara T, Okamoto T, Suzuki R, Yoshii K, Kurosu T, Igarashi M, Aoki H, Sakoda Y, Matsuura Y. PLoS Pathog. 2017 Jun 23;13(6):e1006475. doi: 10.1371/journal.ppat.1006475. eCollection 2017 Jun. PubMed
Generation of Genetically Modified Mice through the Microinjection of Oocytes. Delerue F, Ittner LM. J Vis Exp. 2017 Jun 15;(124). doi: 10.3791/55765. PubMed
Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells. Sternburg EL, Dias KC, Karginov FV. J Vis Exp. 2017 Jun 16;(124). doi: 10.3791/55903. PubMed
TSPAN12 Is a Norrin Co-receptor that Amplifies Frizzled4 Ligand Selectivity and Signaling. Lai MB, Zhang C, Shi J, Johnson V, Khandan L, McVey J, Klymkowsky MW, Chen Z, Junge HJ. Cell Rep. 2017 Jun 27;19(13):2809-2822. doi: 10.1016/j.celrep.2017.06.004. PubMed Associated Plasmids pcDNA 3.3 HA-hTSPAN12 pcDNA 3.3 HA-TSPAN12^11-LEL pcDNA 3.3 EGFP pcDNA 3.3 ss-V5-hFZD4 (aa 37-537) pcDNA3.3 ss-3xFLAG-hLRP5 (aa 32-1615) More ...
CEP19 cooperates with FOP and CEP350 to drive early steps in the ciliogenesis programme. Mojarad BA, Gupta GD, Hasegan M, Goudiam O, Basto R, Gingras AC, Pelletier L. Open Biol. 2017 Jun;7(6). pii: rsob.170114. doi: 10.1098/rsob.170114. PubMed
A novel humanized mouse lacking murine P450 oxidoreductase for studying human drug metabolism. Barzi M, Pankowicz FP, Zorman B, Liu X, Legras X, Yang D, Borowiak M, Bissig-Choisat B, Sumazin P, Li F, Bissig KD. Nat Commun. 2017 Jun 28;8(1):39. doi: 10.1038/s41467-017-00049-x. PubMed
Multiplexed gene editing and protein over-expression using a Tobacco mosaic virus viral vector. Cody W, Scholthof HB, Mirkov TE. Plant Physiol. 2017 Jun 29. pii: pp.00411.2017. doi: 10.1104/pp.17.00411. PubMed
Inherited determinants of early recurrent somatic mutations in prostate cancer. Romanel A, Garritano S, Stringa B, Blattner M, Dalfovo D, Chakravarty D, Soong D, Cotter KA, Petris G, Dhingra P, Gasperini P, Cereseto A, Elemento O, Sboner A, Khurana E, Inga A, Rubin MA, Demichelis F. Nat Commun. 2017 Jun 29;8(1):48. doi: 10.1038/s41467-017-00046-0. PubMed
Comparative analysis of chimeric ZFP-, TALE- and Cas9-piggyBac transposases for integration into a single locus in human cells. Luo W, Galvan DL, Woodard LE, Dorset D, Levy S, Wilson MH. Nucleic Acids Res. 2017 Jun 29. doi: 10.1093/nar/gkx572. PubMed
Norrin-induced Frizzled4 endocytosis and endo-lysosomal trafficking control retinal angiogenesis and barrier function. Zhang C, Lai MB, Khandan L, Lee LA, Chen Z, Junge HJ. Nat Commun. 2017 Jul 4;8:16050. doi: 10.1038/ncomms16050. PubMed
A convenient method to pre-screen candidate guide RNAs for CRISPR/Cas9 gene editing by NHEJ-mediated integration of a 'self-cleaving' GFP-expression plasmid. Talas A, Kulcsar PI, Weinhardt N, Borsy A, Toth E, Szebenyi K, Krausz SL, Huszar K, Vida I, Sturm A, Gordos B, Hoffmann OI, Bencsura P, Nyeste A, Ligeti Z, Fodor E, Welker E. DNA Res. 2017 Jun 30. doi: 10.1093/dnares/dsx029. PubMed Associated Plasmids pSc1 pSc2 pSc1-puro pSc1-DD
HSF1 and HSF3 cooperatively regulate the heat shock response in lizards. Takii R, Fujimoto M, Matsuura Y, Wu F, Oshibe N, Takaki E, Katiyar A, Akashi H, Makino T, Kawata M, Nakai A. PLoS One. 2017 Jul 7;12(7):e0180776. doi: 10.1371/journal.pone.0180776. eCollection 2017. PubMed
Somatic chromosomal engineering identifies BCAN-NTRK1 as a potent glioma driver and therapeutic target. Cook PJ, Thomas R, Kannan R, de Leon ES, Drilon A, Rosenblum MK, Scaltriti M, Benezra R, Ventura A. Nat Commun. 2017 Jul 11;8:15987. doi: 10.1038/ncomms15987. PubMed Associated Plasmids Adeno BN Adeno FT
Dual loss of human POLQ and LIG4 abolishes random integration. Saito S, Maeda R, Adachi N. Nat Commun. 2017 Jul 11;8:16112. doi: 10.1038/ncomms16112. PubMed
Putative binding sites for mir-125 family miRNAs in the mouse Lfng 3'UTR affect transcript expression in the segmentation clock, but mir-125a-5p is dispensable for normal somitogenesis. Wahi K, Friesen S, Coppola V, Cole SE. Dev Dyn. 2017 Oct;246(10):740-748. doi: 10.1002/dvdy.24552. Epub 2017 Aug 18. PubMed
Acylglycerol Kinase Mutated in Sengers Syndrome Is a Subunit of the TIM22 Protein Translocase in Mitochondria. Vukotic M, Nolte H, Konig T, Saita S, Ananjew M, Kruger M, Tatsuta T, Langer T. Mol Cell. 2017 Aug 3;67(3):471-483.e7. doi: 10.1016/j.molcel.2017.06.013. Epub 2017 Jul 14. PubMed
Protein kinase D at the Golgi controls NLRP3 inflammasome activation. Zhang Z, Meszaros G, He WT, Xu Y, de Fatima Magliarelli H, Mailly L, Mihlan M, Liu Y, Puig Gamez M, Goginashvili A, Pasquier A, Bielska O, Neven B, Quartier P, Aebersold R, Baumert TF, Georgel P, Han J, Ricci R. J Exp Med. 2017 Sep 4;214(9):2671-2693. doi: 10.1084/jem.20162040. Epub 2017 Jul 17. PubMed
A BAG3 chaperone complex maintains cardiomyocyte function during proteotoxic stress. Judge LM, Perez-Bermejo JA, Truong A, Ribeiro AJ, Yoo JC, Jensen CL, Mandegar MA, Huebsch N, Kaake RM, So PL, Srivastava D, Pruitt BL, Krogan NJ, Conklin BR. JCI Insight. 2017 Jul 20;2(14). pii: 94623. doi: 10.1172/jci.insight.94623. eCollection 2017 Jul 20. PubMed
Evaluation of ATM heterozygous mutations underlying individual differences in radiosensitivity using genome editing in human cultured cells. Royba E, Miyamoto T, Natsuko Akutsu S, Hosoba K, Tauchi H, Kudo Y, Tashiro S, Yamamoto T, Matsuura S. Sci Rep. 2017 Jul 20;7(1):5996. doi: 10.1038/s41598-017-06393-8. PubMed
Tissue-specific CTCF-cohesin-mediated chromatin architecture delimits enhancer interactions and function in vivo. Hanssen LLP, Kassouf MT, Oudelaar AM, Biggs D, Preece C, Downes DJ, Gosden M, Sharpe JA, Sloane-Stanley JA, Hughes JR, Davies B, Higgs DR. Nat Cell Biol. 2017 Aug;19(8):952-961. doi: 10.1038/ncb3573. Epub 2017 Jul 24. PubMed
Hepatitis B virus prevents excessive viral production via reduction of cell death-inducing DFF45-like effectors. Yasumoto J, Kasai H, Yoshimura K, Otoguro T, Watashi K, Wakita T, Yamashita A, Tanaka T, Takeda S, Moriishi K. J Gen Virol. 2017 Jul;98(7):1762-1773. doi: 10.1099/jgv.0.000813. PubMed
Echinomycin inhibits adipogenesis in 3T3-L1 cells in a HIF-independent manner. Yamaguchi J, Tanaka T, Saito H, Nomura S, Aburatani H, Waki H, Kadowaki T, Nangaku M. Sci Rep. 2017 Jul 26;7(1):6516. doi: 10.1038/s41598-017-06761-4. PubMed
GA-Binding Protein Alpha Is Involved in the Survival of Mouse Embryonic Stem Cells. Ueda A, Akagi T, Yokota T. Stem Cells. 2017 Nov;35(11):2229-2238. doi: 10.1002/stem.2673. Epub 2017 Aug 8. PubMed
Immunocytochemistry and fluorescence imaging efficiently identify individual neurons with CRISPR/Cas9-mediated gene disruption in primary cortical cultures. Tsunematsu H, Uyeda A, Yamamoto N, Sugo N. BMC Neurosci. 2017 Aug 1;18(1):55. doi: 10.1186/s12868-017-0377-9. PubMed
Reactivity of human AGO2 monoclonal antibody 11A9 with the SWI/SNF complex: A case study for rigorously defining antibody selectivity. van Eijl RAPM, van den Brand T, Nguyen LN, Mulder KW. Sci Rep. 2017 Aug 4;7(1):7278. doi: 10.1038/s41598-017-07539-4. PubMed
Trap(Seq): An RNA Sequencing-Based Pipeline for the Identification of Gene-Trap Insertions in Mammalian Cells. Mayor-Ruiz C, Dominguez O, Fernandez-Capetillo O. J Mol Biol. 2017 Sep 1;429(18):2780-2789. doi: 10.1016/j.jmb.2017.07.020. Epub 2017 Aug 4. PubMed
Early and consistent overexpression of ADRM1 in ovarian high-grade serous carcinoma. Jiang RT, Yemelyanova A, Xing D, Anchoori RK, Hamazaki J, Murata S, Seidman JD, Wang TL, Roden RBS. J Ovarian Res. 2017 Aug 7;10(1):53. doi: 10.1186/s13048-017-0347-y. PubMed
Atg2A/B deficiency switches cytoprotective autophagy to non-canonical caspase-8 activation and apoptosis. Tang Z, Takahashi Y, Chen C, Liu Y, He H, Tsotakos N, Serfass JM, Gebru MT, Chen H, Young MM, Wang HG. Cell Death Differ. 2017 Dec;24(12):2127-2138. doi: 10.1038/cdd.2017.133. Epub 2017 Aug 11. PubMed
Reck and Gpr124 Are Essential Receptor Cofactors for Wnt7a/Wnt7b-Specific Signaling in Mammalian CNS Angiogenesis and Blood-Brain Barrier Regulation. Cho C, Smallwood PM, Nathans J. Neuron. 2017 Aug 30;95(5):1056-1073.e5. doi: 10.1016/j.neuron.2017.07.031. Epub 2017 Aug 10. PubMed
Myc Regulates Chromatin Decompaction and Nuclear Architecture during B Cell Activation. Kieffer-Kwon KR, Nimura K, Rao SSP, Xu J, Jung S, Pekowska A, Dose M, Stevens E, Mathe E, Dong P, Huang SC, Ricci MA, Baranello L, Zheng Y, Tomassoni Ardori F, Resch W, Stavreva D, Nelson S, McAndrew M, Casellas A, Finn E, Gregory C, St Hilaire BG, Johnson SM, Dubois W, Cosma MP, Batchelor E, Levens D, Phair RD, Misteli T, Tessarollo L, Hager G, Lakadamyali M, Liu Z, Floer M, Shroff H, Aiden EL, Casellas R. Mol Cell. 2017 Aug 17;67(4):566-578.e10. doi: 10.1016/j.molcel.2017.07.013. Epub 2017 Aug 10. PubMed
Listeria monocytogenes and Shigella flexneri Activate the NLRP1B Inflammasome. Neiman-Zenevich J, Stuart S, Abdel-Nour M, Girardin SE, Mogridge J. Infect Immun. 2017 Oct 18;85(11). pii: IAI.00338-17. doi: 10.1128/IAI.00338-17. Print 2017 Nov. PubMed
Identification of CMTM6 and CMTM4 as PD-L1 protein regulators. Mezzadra R, Sun C, Jae LT, Gomez-Eerland R, de Vries E, Wu W, Logtenberg MEW, Slagter M, Rozeman EA, Hofland I, Broeks A, Horlings HM, Wessels LFA, Blank CU, Xiao Y, Heck AJR, Borst J, Brummelkamp TR, Schumacher TNM. Nature. 2017 Sep 7;549(7670):106-110. doi: 10.1038/nature23669. Epub 2017 Aug 16. PubMed
ADAM10-Mediated ICOS Ligand Shedding on B Cells Is Necessary for Proper T Cell ICOS Regulation and T Follicular Helper Responses. Lownik JC, Luker AJ, Damle SR, Cooley LF, El Sayed R, Hutloff A, Pitzalis C, Martin RK, El Shikh MEM, Conrad DH. J Immunol. 2017 Oct 1;199(7):2305-2315. doi: 10.4049/jimmunol.1700833. Epub 2017 Aug 16. PubMed
Small molecules enhance CRISPR/Cas9-mediated homology-directed genome editing in primary cells. Li G, Zhang X, Zhong C, Mo J, Quan R, Yang J, Liu D, Li Z, Yang H, Wu Z. Sci Rep. 2017 Aug 21;7(1):8943. doi: 10.1038/s41598-017-09306-x. PubMed
B-cell Receptor Signaling Regulates Metabolism in Chronic Lymphocytic Leukemia. Vangapandu HV, Havranek O, Ayres ML, Kaipparettu BA, Balakrishnan K, Wierda WG, Keating MJ, Davis RE, Stellrecht CM, Gandhi V. Mol Cancer Res. 2017 Dec;15(12):1692-1703. doi: 10.1158/1541-7786.MCR-17-0026. Epub 2017 Aug 23. PubMed
Functional significance of rare neuroligin 1 variants found in autism. Nakanishi M, Nomura J, Ji X, Tamada K, Arai T, Takahashi E, Bucan M, Takumi T. PLoS Genet. 2017 Aug 25;13(8):e1006940. doi: 10.1371/journal.pgen.1006940. eCollection 2017 Aug. PubMed
Esco1 and Esco2 regulate distinct cohesin functions during cell cycle progression. Alomer RM, da Silva EML, Chen J, Piekarz KM, McDonald K, Sansam CG, Sansam CL, Rankin S. Proc Natl Acad Sci U S A. 2017 Sep 12;114(37):9906-9911. doi: 10.1073/pnas.1708291114. Epub 2017 Aug 28. PubMed
Multiplexed sgRNA Expression Allows Versatile Single Nonrepetitive DNA Labeling and Endogenous Gene Regulation. Shao S, Chang L, Sun Y, Hou Y, Fan X, Sun Y. ACS Synth Biol. 2017 Sep 7. doi: 10.1021/acssynbio.7b00268. PubMed Associated Plasmids pMulti-sgRNA-LacZ pMulti-sgRNA-LacZ-DsRed psgRNA-ccdB
Postnatal Cardiac Gene Editing Using CRISPR/Cas9 With AAV9-Mediated Delivery of Short Guide RNAs Results in Mosaic Gene Disruption. Johansen AK, Molenaar B, Versteeg D, Leitoguinho AR, Demkes C, Spanjaard B, de Ruiter H, Akbari Moqadam F, Kooijman L, Zentilin L, Giacca M, van Rooij E. Circ Res. 2017 Oct 27;121(10):1168-1181. doi: 10.1161/CIRCRESAHA.116.310370. Epub 2017 Aug 29. PubMed Associated Plasmids Myh6-Cas9
Deficiency in Kelch protein Klhl31 causes congenital myopathy in mice. Papizan JB, Garry GA, Brezprozvannaya S, McAnally JR, Bassel-Duby R, Liu N, Olson EN. J Clin Invest. 2017 Oct 2;127(10):3730-3740. doi: 10.1172/JCI93445. Epub 2017 Sep 5. PubMed
Haploid Mammalian Genetic Screen Identifies UBXD8 as a Key Determinant of HMGCR Degradation and Cholesterol Biosynthesis. Loregger A, Raaben M, Tan J, Scheij S, Moeton M, van den Berg M, Gelberg-Etel H, Stickel E, Roitelman J, Brummelkamp T, Zelcer N. Arterioscler Thromb Vasc Biol. 2017 Nov;37(11):2064-2074. doi: 10.1161/ATVBAHA.117.310002. Epub 2017 Sep 7. PubMed
Human microcephaly ASPM protein is a spindle pole-focusing factor that functions redundantly with CDK5RAP2. Tungadi EA, Ito A, Kiyomitsu T, Goshima G. J Cell Sci. 2017 Nov 1;130(21):3676-3684. doi: 10.1242/jcs.203703. Epub 2017 Sep 7. PubMed
Distinct roles of neuronal and microglial CB2 cannabinoid receptors in the mouse hippocampus. Li Y, Kim J. Neuroscience. 2017 Sep 6. pii: S0306-4522(17)30629-2. doi: 10.1016/j.neuroscience.2017.08.053. PubMed Associated Plasmids pAAV-CBh-DIO-EGFP pAAV-CBh-DIO-CB2-EGFP pAAV-U6-gRNA-CBh-mCherry pAAV-U6-CB2gRNA-CBh-mCherry
CRISPR-Cas9-based photoactivatable transcription systems to induce neuronal differentiation. Nihongaki Y, Furuhata Y, Otabe T, Hasegawa S, Yoshimoto K, Sato M. Nat Methods. 2017 Oct;14(10):963-966. doi: 10.1038/nmeth.4430. Epub 2017 Sep 11. PubMed
Prion protein is required for tumor necrosis factor alpha (TNFalpha)-triggered nuclear factor kappaB (NF-kappaB) signaling and cytokine production. Wu GR, Mu TC, Gao ZX, Wang J, Sy MS, Li CY. J Biol Chem. 2017 Nov 17;292(46):18747-18759. doi: 10.1074/jbc.M117.787283. Epub 2017 Sep 12. PubMed
Syk-dependent tyrosine phosphorylation of 3BP2 is required for optimal FcRgamma-mediated phagocytosis and chemokine expression in U937 cells. Chihara K, Kato Y, Yoshiki H, Takeuchi K, Fujieda S, Sada K. Sci Rep. 2017 Sep 13;7(1):11480. doi: 10.1038/s41598-017-11915-5. PubMed
Centriole triplet microtubules are required for stable centriole formation and inheritance in human cells. Wang JT, Kong D, Hoerner CR, Loncarek J, Stearns T. Elife. 2017 Sep 14;6. pii: 29061. doi: 10.7554/eLife.29061. PubMed
Massive reshaping of genome-nuclear lamina interactions during oncogene-induced senescence. Lenain C, de Graaf CA, Pagie L, Visser NL, de Haas M, de Vries SS, Peric-Hupkes D, van Steensel B, Peeper DS. Genome Res. 2017 Oct;27(10):1634-1644. doi: 10.1101/gr.225763.117. Epub 2017 Sep 15. PubMed
Efficient gene editing via non-viral delivery of CRISPR-Cas9 system using polymeric and hybrid microcarriers. Timin AS, Muslimov AR, Lepik KV, Epifanovskaya OS, Shakirova AI, Mock U, Riecken K, Okilova MV, Sergeev VS, Afanasyev BV, Fehse B, Sukhorukov GB. Nanomedicine. 2018 Jan;14(1):97-108. doi: 10.1016/j.nano.2017.09.001. Epub 2017 Sep 14. PubMed
USH2A Gene Editing Using the CRISPR System. Fuster-Garcia C, Garcia-Garcia G, Gonzalez-Romero E, Jaijo T, Sequedo MD, Ayuso C, Vazquez-Manrique RP, Millan JM, Aller E. Mol Ther Nucleic Acids. 2017 Sep 15;8:529-541. doi: 10.1016/j.omtn.2017.08.003. Epub 2017 Aug 12. PubMed
CRISPR/Cas9 Engineering of Adult Mouse Liver Demonstrates That the Dnajb1-Prkaca Gene Fusion Is Sufficient to Induce Tumors Resembling Fibrolamellar Hepatocellular Carcinoma. Engelholm LH, Riaz A, Serra D, Dagnaes-Hansen F, Johansen JV, Santoni-Rugiu E, Hansen SH, Niola F, Frodin M. Gastroenterology. 2017 Dec;153(6):1662-1673.e10. doi: 10.1053/j.gastro.2017.09.008. Epub 2017 Sep 18. PubMed
Immunosurveillance and Immunoediting of Breast Cancer via Class I MHC Receptors. Tu MM, Rahim MMA, Sayed C, Mahmoud AB, Makrigiannis AP. Cancer Immunol Res. 2017 Nov;5(11):1016-1028. doi: 10.1158/2326-6066.CIR-17-0056. Epub 2017 Sep 18. PubMed
In vivo mouse and live cell STED microscopy of neuronal actin plasticity using far-red emitting fluorescent proteins. Wegner W, Ilgen P, Gregor C, van Dort J, Mott AC, Steffens H, Willig KI. Sci Rep. 2017 Sep 18;7(1):11781. doi: 10.1038/s41598-017-11827-4. PubMed
Generation of an OCT4 reporter human induced pluripotent stem cell line using CRISPR/SpCas9. Balboa D, Weltner J, Novik Y, Eurola S, Wartiovaara K, Otonkoski T. Stem Cell Res. 2017 Aug;23:105-108. doi: 10.1016/j.scr.2017.07.006. Epub 2017 Jul 11. PubMed Associated Plasmids pUC19-OCT4-T2A-NLS-EmGFP-P2A-Puro CAG-Cas9
Enhancing CRISPR/Cas9-mediated homology-directed repair in mammalian cells by expressing Saccharomyces cerevisiae Rad52. Shao S, Ren C, Liu Z, Bai Y, Chen Z, Wei Z, Wang X, Zhang Z, Xu K. Int J Biochem Cell Biol. 2017 Nov;92:43-52. doi: 10.1016/j.biocel.2017.09.012. Epub 2017 Sep 18. PubMed Associated Plasmids pScRad52-SpCas9 (pXK-1646) pHDR-eGFP (pXK-1647)
Correction of beta-thalassemia mutant by base editor in human embryos. Liang P, Ding C, Sun H, Xie X, Xu Y, Zhang X, Sun Y, Xiong Y, Ma W, Liu Y, Wang Y, Fang J, Liu D, Songyang Z, Zhou C, Huang J. Protein Cell. 2017 Nov;8(11):811-822. doi: 10.1007/s13238-017-0475-6. Epub 2017 Sep 23. PubMed
Generation of a novel Antibody-Drug Conjugate targeting endosialin: potent and durable antitumor response in sarcoma. Capone E, Piccolo E, Fichera I, Ciufici P, Barcaroli D, Sala A, De Laurenzi V, Iacobelli V, Iacobelli S, Sala G. Oncotarget. 2017 Jul 22;8(36):60368-60377. doi: 10.18632/oncotarget.19499. eCollection 2017 Sep 1. PubMed
Genome editing reveals a role for OCT4 in human embryogenesis. Fogarty NME, McCarthy A, Snijders KE, Powell BE, Kubikova N, Blakeley P, Lea R, Elder K, Wamaitha SE, Kim D, Maciulyte V, Kleinjung J, Kim JS, Wells D, Vallier L, Bertero A, Turner JMA, Niakan KK. Nature. 2017 Oct 5;550(7674):67-73. doi: 10.1038/nature24033. Epub 2017 Sep 20. PubMed
Regulation of DNA repair pathway choice in S and G2 phases by the NHEJ inhibitor CYREN. Arnoult N, Correia A, Ma J, Merlo A, Garcia-Gomez S, Maric M, Tognetti M, Benner CW, Boulton SJ, Saghatelian A, Karlseder J. Nature. 2017 Sep 20;549(7673):548-552. doi: 10.1038/nature24023. PubMed Associated Plasmids pLCN DSB Repair Reporter (DRR) pCAGGS DRR mCherry Donor EF1a BFP pCAGGS Donor mClover-LMNA pUC CBA-SpCas9.EF1a-BFP.sgLMNA pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair1 More ...
Nesprin-1alpha-Dependent Microtubule Nucleation from the Nuclear Envelope via Akap450 Is Necessary for Nuclear Positioning in Muscle Cells. Gimpel P, Lee YL, Sobota RM, Calvi A, Koullourou V, Patel R, Mamchaoui K, Nedelec F, Shackleton S, Schmoranzer J, Burke B, Cadot B, Gomes ER. Curr Biol. 2017 Oct 9;27(19):2999-3009.e9. doi: 10.1016/j.cub.2017.08.031. Epub 2017 Sep 28. PubMed
Histone H3K4 monomethylation catalyzed by Trr and mammalian COMPASS-like proteins at enhancers is dispensable for development and viability. Rickels R, Herz HM, Sze CC, Cao K, Morgan MA, Collings CK, Gause M, Takahashi YH, Wang L, Rendleman EJ, Marshall SA, Krueger A, Bartom ET, Piunti A, Smith ER, Abshiru NA, Kelleher NL, Dorsett D, Shilatifard A. Nat Genet. 2017 Nov;49(11):1647-1653. doi: 10.1038/ng.3965. Epub 2017 Oct 2. PubMed
t-BuOOH induces ferroptosis in human and murine cell lines. Wenz C, Faust D, Linz B, Turmann C, Nikolova T, Bertin J, Gough P, Wipf P, Schroder AS, Krautwald S, Dietrich C. Arch Toxicol. 2018 Feb;92(2):759-775. doi: 10.1007/s00204-017-2066-y. Epub 2017 Oct 3. PubMed
H2AX facilitates classical non-homologous end joining at the expense of limited nucleotide loss at repair junctions. Feng YL, Xiang JF, Liu SC, Guo T, Yan GF, Feng Y, Kong N, Li HD, Huang Y, Lin H, Cai XJ, Xie AY. Nucleic Acids Res. 2017 Oct 13;45(18):10614-10633. doi: 10.1093/nar/gkx715. PubMed
RUNX2 expression in thyroid and breast cancer requires the cooperation of three non-redundant enhancers under the control of BRD4 and c-JUN. Sancisi V, Manzotti G, Gugnoni M, Rossi T, Gandolfi G, Gobbi G, Torricelli F, Catellani F, Faria do Valle I, Remondini D, Castellani G, Ragazzi M, Piana S, Ciarrocchi A. Nucleic Acids Res. 2017 Nov 2;45(19):11249-11267. doi: 10.1093/nar/gkx802. PubMed
Intragenic Enhancers Attenuate Host Gene Expression. Cinghu S, Yang P, Kosak JP, Conway AE, Kumar D, Oldfield AJ, Adelman K, Jothi R. Mol Cell. 2017 Oct 5;68(1):104-117.e6. doi: 10.1016/j.molcel.2017.09.010. PubMed
Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage. Kulcsar PI, Talas A, Huszar K, Ligeti Z, Toth E, Weinhardt N, Fodor E, Welker E. Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8. PubMed Associated Plasmids pmCherry_gRNA pX330-Flag-SpCas9-HF1 (without sgRNA) HeFm1SpCas9 HeFm2SpCas9 pX330-HA-dSpCas9 More ...
CRISPR/Cas9-induced transgene insertion and telomere-associated truncation of a single human chromosome for chromosome engineering in CHO and A9 cells. Uno N, Hiramatsu K, Uno K, Komoto S, Kazuki Y, Oshimura M. Sci Rep. 2017 Oct 6;7(1):12739. doi: 10.1038/s41598-017-10418-7. PubMed
CRISPR/Cas9-mediated knockout of myostatin in Chinese indigenous Erhualian pigs. Wang K, Tang X, Xie Z, Zou X, Li M, Yuan H, Guo N, Ouyang H, Jiao H, Pang D. Transgenic Res. 2017 Dec;26(6):799-805. doi: 10.1007/s11248-017-0044-z. Epub 2017 Oct 9. PubMed
A point mutation in the ion conduction pore of AMPA receptor GRIA3 causes dramatically perturbed sleep patterns as well as intellectual disability. Davies B, Brown LA, Cais O, Watson J, Clayton AJ, Chang VT, Biggs D, Preece C, Hernandez-Pliego P, Krohn J, Bhomra A, Twigg SRF, Rimmer A, Kanapin A, Sen A, Zaiwalla Z, McVean G, Foster R, Donnelly P, Taylor JC, Blair E, Nutt D, Aricescu AR, Greger IH, Peirson SN, Flint J, Martin HC. Hum Mol Genet. 2017 Oct 15;26(20):3869-3882. doi: 10.1093/hmg/ddx270. PubMed
Radically truncated MeCP2 rescues Rett syndrome-like neurological defects. Tillotson R, Selfridge J, Koerner MV, Gadalla KKE, Guy J, De Sousa D, Hector RD, Cobb SR, Bird A. Nature. 2017 Oct 19;550(7676):398-401. doi: 10.1038/nature24058. Epub 2017 Oct 11. PubMed Associated Plasmids pEGFP-N1_MeCP2(WT) pEGFP-N1_MeCP2(R111G) pEGFP-N1_MeCP2(R306C) pEGFP-N1_MeCP2(deltaN) pEGFP-N1_MeCP2(deltaNC) More ...
Diverse Viruses Require the Calcium Transporter SPCA1 for Maturation and Spread. Hoffmann HH, Schneider WM, Blomen VA, Scull MA, Hovnanian A, Brummelkamp TR, Rice CM. Cell Host Microbe. 2017 Oct 11;22(4):460-470.e5. doi: 10.1016/j.chom.2017.09.002. PubMed
A long-range cis-regulatory element for class I odorant receptor genes. Iwata T, Niimura Y, Kobayashi C, Shirakawa D, Suzuki H, Enomoto T, Touhara K, Yoshihara Y, Hirota J. Nat Commun. 2017 Oct 12;8(1):885. doi: 10.1038/s41467-017-00870-4. PubMed
Systematic Analysis of Human Cells Lacking ATG8 Proteins Uncovers Roles for GABARAPs and the CCZ1/MON1 Regulator C18orf8/RMC1 in Macroautophagic and Selective Autophagic Flux. Vaites LP, Paulo JA, Huttlin EL, Harper JW. Mol Cell Biol. 2017 Dec 13;38(1). pii: MCB.00392-17. doi: 10.1128/MCB.00392-17. Print 2018 Jan 1. PubMed
Control of growth and gut maturation by HoxD genes and the associated lncRNA Haglr. Zakany J, Darbellay F, Mascrez B, Necsulea A, Duboule D. Proc Natl Acad Sci U S A. 2017 Oct 31;114(44):E9290-E9299. doi: 10.1073/pnas.1712511114. Epub 2017 Oct 17. PubMed
Polycomb group RING finger proteins 3/5 activate transcription via an interaction with the pluripotency factor Tex10 in embryonic stem cells. Zhao W, Huang Y, Zhang J, Liu M, Ji H, Wang C, Cao N, Li C, Xia Y, Jiang Q, Qin J. J Biol Chem. 2017 Dec 29;292(52):21527-21537. doi: 10.1074/jbc.M117.804054. Epub 2017 Oct 20. PubMed
Human Globozoospermia-Related Gene Spata16 Is Required for Sperm Formation Revealed by CRISPR/Cas9-Mediated Mouse Models. Fujihara Y, Oji A, Larasati T, Kojima-Kita K, Ikawa M. Int J Mol Sci. 2017 Oct 21;18(10). pii: ijms18102208. doi: 10.3390/ijms18102208. PubMed
In Vivo Translatome Profiling in Spinal Muscular Atrophy Reveals a Role for SMN Protein in Ribosome Biology. Bernabo P, Tebaldi T, Groen EJN, Lane FM, Perenthaler E, Mattedi F, Newbery HJ, Zhou H, Zuccotti P, Potrich V, Shorrock HK, Muntoni F, Quattrone A, Gillingwater TH, Viero G. Cell Rep. 2017 Oct 24;21(4):953-965. doi: 10.1016/j.celrep.2017.10.010. PubMed
Baf53a is involved in survival of mouse ES cells, which can be compensated by Baf53b. Zhu B, Ueda A, Song X, Horike SI, Yokota T, Akagi T. Sci Rep. 2017 Oct 25;7(1):14059. doi: 10.1038/s41598-017-14362-4. PubMed
PAXX and Xlf interplay revealed by impaired CNS development and immunodeficiency of double KO mice. Abramowski V, Etienne O, Elsaid R, Yang J, Berland A, Kermasson L, Roch B, Musilli S, Moussu JP, Lipson-Ruffert K, Revy P, Cumano A, Boussin FD, de Villartay JP. Cell Death Differ. 2018 Feb;25(2):444-452. doi: 10.1038/cdd.2017.184. Epub 2017 Oct 27. PubMed
APELA promotes tumour growth and cell migration in ovarian cancer in a p53-dependent manner. Yi Y, Tsai SH, Cheng JC, Wang EY, Anglesio MS, Cochrane DR, Fuller M, Gibb EA, Wei W, Huntsman DG, Karsan A, Hoodless PA. Gynecol Oncol. 2017 Dec;147(3):663-671. doi: 10.1016/j.ygyno.2017.10.016. Epub 2017 Oct 24. PubMed
Integrated Proteomic and Transcriptomic Analysis Reveals Long Noncoding RNA HOX Transcript Antisense Intergenic RNA (HOTAIR) Promotes Hepatocellular Carcinoma Cell Proliferation by Regulating Opioid Growth Factor Receptor (OGFr). Wu Y, Xiong Q, Li S, Yang X, Ge F. Mol Cell Proteomics. 2018 Jan;17(1):146-159. doi: 10.1074/mcp.RA117.000277. Epub 2017 Oct 27. PubMed
Murine pluripotent stem cells with a homozygous knockout of Foxg1 show reduced differentiation towards cortical progenitors in vitro. Mall EM, Herrmann D, Niemann H. Stem Cell Res. 2017 Dec;25:50-60. doi: 10.1016/j.scr.2017.10.012. Epub 2017 Oct 19. PubMed
A cryptic Tudor domain links BRWD2/PHIP to COMPASS-mediated histone H3K4 methylation. Morgan MAJ, Rickels RA, Collings CK, He X, Cao K, Herz HM, Cozzolino KA, Abshiru NA, Marshall SA, Rendleman EJ, Sze CC, Piunti A, Kelleher NL, Savas JN, Shilatifard A. Genes Dev. 2017 Oct 1;31(19):2003-2014. doi: 10.1101/gad.305201.117. PubMed
Modification of single-nucleotide polymorphism in a fully humanized CYP3A mouse by genome editing technology. Abe S, Kobayashi K, Oji A, Sakuma T, Kazuki K, Takehara S, Nakamura K, Okada A, Tsukazaki Y, Senda N, Honma K, Yamamoto T, Ikawa M, Chiba K, Oshimura M, Kazuki Y. Sci Rep. 2017 Nov 9;7(1):15189. doi: 10.1038/s41598-017-15033-0. PubMed
Distinct functional consequences of ECEL1/DINE missense mutations in the pathogenesis of congenital contracture disorders. Nagata K, Takahashi M, Kiryu-Seo S, Kiyama H, Saido TC. Acta Neuropathol Commun. 2017 Nov 13;5(1):83. doi: 10.1186/s40478-017-0486-9. PubMed
Autophagosomal Content Profiling Reveals an LC3C-Dependent Piecemeal Mitophagy Pathway. Le Guerroue F, Eck F, Jung J, Starzetz T, Mittelbronn M, Kaulich M, Behrends C. Mol Cell. 2017 Nov 16;68(4):786-796.e6. doi: 10.1016/j.molcel.2017.10.029. PubMed
The role of N-terminal segment and membrane association in MyD88-mediated signaling. Avbelj M, Panter G, Jerala R. Biochem Biophys Res Commun. 2018 Jan 1;495(1):878-883. doi: 10.1016/j.bbrc.2017.11.099. Epub 2017 Nov 16. PubMed
Histone methyltransferase MMSET promotes AID-mediated DNA breaks at the donor switch region during class switch recombination. Nguyen HV, Dong J, Panchakshari RA, Kumar V, Alt FW, Bories JC. Proc Natl Acad Sci U S A. 2017 Dec 5;114(49):E10560-E10567. doi: 10.1073/pnas.1701366114. Epub 2017 Nov 20. PubMed
Injury Induces Endogenous Reprogramming and Dedifferentiation of Neuronal Progenitors to Multipotency. Lin B, Coleman JH, Peterson JN, Zunitch MJ, Jang W, Herrick DB, Schwob JE. Cell Stem Cell. 2017 Dec 7;21(6):761-774.e5. doi: 10.1016/j.stem.2017.09.008. Epub 2017 Nov 22. PubMed
Crispr-Cas9 engineered osteogenesis imperfecta type V leads to severe skeletal deformities and perinatal lethality in mice. Rauch F, Geng Y, Lamplugh L, Hekmatnejad B, Gaumond MH, Penney J, Yamanaka Y, Moffatt P. Bone. 2018 Feb;107:131-142. doi: 10.1016/j.bone.2017.11.013. Epub 2017 Nov 22. PubMed
CRISPR/Cas9-mediated genome editing in naive human embryonic stem cells. Jacobs EZ, Warrier S, Volders PJ, D'haene E, Van Lombergen E, Vantomme L, Van der Jeught M, Heindryckx B, Menten B, Vergult S. Sci Rep. 2017 Nov 30;7(1):16650. doi: 10.1038/s41598-017-16932-y. PubMed
The Pore-Forming Protein Gasdermin D Regulates Interleukin-1 Secretion from Living Macrophages. Evavold CL, Ruan J, Tan Y, Xia S, Wu H, Kagan JC. Immunity. 2018 Jan 16;48(1):35-44.e6. doi: 10.1016/j.immuni.2017.11.013. Epub 2017 Nov 28. PubMed
Transcriptional landscape of a RET(C634Y)-mutated iPSC and its CRISPR-corrected isogenic control reveals the putative role of EGR1 transcriptional program in the development of multiple endocrine neoplasia type 2A-associated cancers. Hadoux J, Desterke C, Feraud O, Guibert M, De Rose RF, Opolon P, Divers D, Gobbo E, Griscelli F, Schlumberger M, Bennaceur-Griscelli A, Turhan AG. Stem Cell Res. 2018 Jan;26:8-16. doi: 10.1016/j.scr.2017.11.015. Epub 2017 Nov 23. PubMed
Linear Integration of ERK Activity Predominates over Persistence Detection in Fra-1 Regulation. Gillies TE, Pargett M, Minguet M, Davies AE, Albeck JG. Cell Syst. 2017 Dec 27;5(6):549-563.e5. doi: 10.1016/j.cels.2017.10.019. Epub 2017 Nov 29. PubMed
CRISPR/Cas9 Editing of the Mouse Thra Gene Produces Models with Variable Resistance to Thyroid Hormone. Markossian S, Guyot R, Richard S, Teixeira M, Aguilera N, Bouchet M, Plateroti M, Guan W, Gauthier K, Aubert D, Flamant F. Thyroid. 2018 Jan;28(1):139-150. doi: 10.1089/thy.2017.0389. Epub 2018 Jan 2. PubMed
A Chemical-Genetic Approach Reveals the Distinct Roles of GSK3alpha and GSK3beta in Regulating Embryonic Stem Cell Fate. Chen X, Wang R, Liu X, Wu Y, Zhou T, Yang Y, Perez A, Chen YC, Hu L, Chadarevian JP, Assadieskandar A, Zhang C, Ying QL. Dev Cell. 2017 Dec 4;43(5):563-576.e4. doi: 10.1016/j.devcel.2017.11.007. PubMed
Versatile single-step-assembly CRISPR/Cas9 vectors for dual gRNA expression. Adikusuma F, Pfitzner C, Thomas PQ. PLoS One. 2017 Dec 6;12(12):e0187236. doi: 10.1371/journal.pone.0187236. eCollection 2017. PubMed Associated Plasmids pDG330 pDG335 pDG458 pDG459 pDG461 More ...
PRPF8 is important for BRCA1-mediated homologous recombination. Onyango DO, Lee G, Stark JM. Oncotarget. 2017 Oct 6;8(55):93319-93337. doi: 10.18632/oncotarget.21555. eCollection 2017 Nov 7. PubMed
A novel mouse model for tracking the fate of CXCR5-expressing T cells. Takebe T, Sakamoto K, Higami Y, Harada Y. Biochem Biophys Res Commun. 2018 Jan 8;495(2):1642-1647. doi: 10.1016/j.bbrc.2017.12.029. Epub 2017 Dec 6. PubMed
EGFR feedback-inhibition by Ran-binding protein 6 is disrupted in cancer. Oldrini B, Hsieh WY, Erdjument-Bromage H, Codega P, Carro MS, Curiel-Garcia A, Campos C, Pourmaleki M, Grommes C, Vivanco I, Rohle D, Bielski CM, Taylor BS, Hollmann TJ, Rosenblum M, Tempst P, Blenis J, Squatrito M, Mellinghoff IK. Nat Commun. 2017 Dec 11;8(1):2035. doi: 10.1038/s41467-017-02185-w. PubMed
Regulation of type I collagen expression by microRNA-29 following ionizing radiation. Yano H, Hamanaka R, Nakamura-Ota M, Zhang JJ, Matsuo N, Yoshioka H. Radiat Environ Biophys. 2018 Mar;57(1):41-54. doi: 10.1007/s00411-017-0723-4. Epub 2017 Dec 11. PubMed
Multimode drug inducible CRISPR/Cas9 devices for transcriptional activation and genome editing. Lu J, Zhao C, Zhao Y, Zhang J, Zhang Y, Chen L, Han Q, Ying Y, Peng S, Ai R, Wang Y. Nucleic Acids Res. 2018 Mar 16;46(5):e25. doi: 10.1093/nar/gkx1222. PubMed Associated Plasmids ScFv-2ERT2-V EF1a-Cas9-2XNES-2ERT2-GCN4-SV40-Zeo ScFv-2ERT2-VPH
SaCas9 Requires 5'-NNGRRT-3' PAM for Sufficient Cleavage and Possesses Higher Cleavage Activity than SpCas9 or FnCpf1 in Human Cells. Xie H, Tang L, He X, Liu X, Zhou C, Liu J, Ge X, Li J, Liu C, Zhao J, Qu J, Song Z, Gu F. Biotechnol J. 2018 Apr;13(4):e1700561. doi: 10.1002/biot.201700561. Epub 2018 Jan 10. PubMed
Site-specific chromosomal gene insertion: Flp recombinase versus Cas9 nuclease. Phan QV, Contzen J, Seemann P, Gossen M. Sci Rep. 2017 Dec 19;7(1):17771. doi: 10.1038/s41598-017-17651-0. PubMed
OTX1 regulates cell cycle progression of neural progenitors in the developing cerebral cortex. Huang B, Li X, Tu X, Zhao W, Zhu D, Feng Y, Si X, Chen JG. J Biol Chem. 2018 Feb 9;293(6):2137-2148. doi: 10.1074/jbc.RA117.001249. Epub 2017 Dec 22. PubMed
Serine/threonine phosphatase 5 (PP5C/PPP5C) regulates the ISOC channel through a PP5C-FKBP51 axis. Hamilton CL, Abney KA, Vasauskas AA, Alexeyev M, Li N, Honkanen RE, Scammell JG, Cioffi DL. Pulm Circ. 2018 Jan-Mar;8(1):2045893217753156. doi: 10.1177/2045893217753156. Epub 2017 Dec 28. PubMed
Reconstruction of the Human Colon Epithelium In Vivo. Sugimoto S, Ohta Y, Fujii M, Matano M, Shimokawa M, Nanki K, Date S, Nishikori S, Nakazato Y, Nakamura T, Kanai T, Sato T. Cell Stem Cell. 2018 Feb 1;22(2):171-176.e5. doi: 10.1016/j.stem.2017.11.012. Epub 2017 Dec 28. PubMed
Generation of FHL2 homozygous knockout lines from human embryonic stem cells by CRISPR/Cas9-mediated ablation. Chang CW, Chang CC, Hsia KC, Tsai SY. Stem Cell Res. 2018 Mar;27:21-24. doi: 10.1016/j.scr.2017.12.015. Epub 2017 Dec 23. PubMed
Targeted knock-in of an scFv-Fc antibody gene into the hprt locus of Chinese hamster ovary cells using CRISPR/Cas9 and CRIS-PITCh systems. Kawabe Y, Komatsu S, Komatsu S, Murakami M, Ito A, Sakuma T, Nakamura T, Yamamoto T, Kamihira M. J Biosci Bioeng. 2018 May;125(5):599-605. doi: 10.1016/j.jbiosc.2017.12.003. Epub 2017 Dec 30. PubMed
Lamp1 Increases the Efficiency of Lassa Virus Infection by Promoting Fusion in Less Acidic Endosomal Compartments. Hulseberg CE, Feneant L, Szymanska KM, White JM. MBio. 2018 Jan 2;9(1). pii: mBio.01818-17. doi: 10.1128/mBio.01818-17. PubMed
Gene correction of HAX1 reversed Kostmann disease phenotype in patient-specific induced pluripotent stem cells. Pittermann E, Lachmann N, MacLean G, Emmrich S, Ackermann M, Gohring G, Schlegelberger B, Welte K, Schambach A, Heckl D, Orkin SH, Cantz T, Klusmann JH. Blood Adv. 2017 Jun 2;1(14):903-914. doi: 10.1182/bloodadvances.2016003798. eCollection 2017 Jun 13. PubMed
A knock-in mouse strain facilitates dynamic tracking and enrichment of MEIS1. Xiang P, Wei W, Hofs N, Clemans-Gibbon J, Maetzig T, Lai CK, Dhillon I, May C, Ruschmann J, Schneider E, Rosten P, Hu K, Kuchenbauer F, Hoodless PA, Humphries RK. Blood Adv. 2017 Nov 7;1(24):2225-2235. doi: 10.1182/bloodadvances.2017010355. eCollection 2017 Nov 14. PubMed
Abnormal Cell Sorting Underlies the Unique X-Linked Inheritance of PCDH19 Epilepsy. Pederick DT, Richards KL, Piltz SG, Kumar R, Mincheva-Tasheva S, Mandelstam SA, Dale RC, Scheffer IE, Gecz J, Petrou S, Hughes JN, Thomas PQ. Neuron. 2018 Jan 3;97(1):59-66.e5. doi: 10.1016/j.neuron.2017.12.005. PubMed
DNA double-strand break response factors influence end-joining features of IgH class switch and general translocation junctions. Panchakshari RA, Zhang X, Kumar V, Du Z, Wei PC, Kao J, Dong J, Alt FW. Proc Natl Acad Sci U S A. 2018 Jan 23;115(4):762-767. doi: 10.1073/pnas.1719988115. Epub 2018 Jan 8. PubMed
SCFAs strongly stimulate PYY production in human enteroendocrine cells. Larraufie P, Martin-Gallausiaux C, Lapaque N, Dore J, Gribble FM, Reimann F, Blottiere HM. Sci Rep. 2018 Jan 8;8(1):74. doi: 10.1038/s41598-017-18259-0. PubMed
TIP60 Complex Inhibits Hepatitis B Virus Transcription. Nishitsuji H, Ujino S, Harada K, Shimotohno K. J Virol. 2018 Feb 26;92(6). pii: JVI.01788-17. doi: 10.1128/JVI.01788-17. Print 2018 Mar 15. PubMed
Improved Cytotoxic T Lymphocyte Responses to Vaccination with Porcine Reproductive and Respiratory Syndrome Virus in 4-1BB Transgenic Pigs. Huang G, Liu X, Duszynski DW, Tang X, El-Ashram S, Liu Z, Suo X, Li Q. Front Immunol. 2017 Dec 18;8:1846. doi: 10.3389/fimmu.2017.01846. eCollection 2017. PubMed
Generation of Optogenetically Modified Adenovirus Vector for Spatiotemporally Controllable Gene Therapy. Takayama K, Mizuguchi H. ACS Chem Biol. 2018 Feb 16;13(2):449-454. doi: 10.1021/acschembio.7b01058. Epub 2018 Jan 12. PubMed
EXD2 governs germ stem cell homeostasis and lifespan by promoting mitoribosome integrity and translation. Silva J, Aivio S, Knobel PA, Bailey LJ, Casali A, Vinaixa M, Garcia-Cao I, Coyaud E, Jourdain AA, Perez-Ferreros P, Rojas AM, Antolin-Fontes A, Samino-Gene S, Raught B, Gonzalez-Reyes A, Ribas de Pouplana L, Doherty AJ, Yanes O, Stracker TH. Nat Cell Biol. 2018 Feb;20(2):162-174. doi: 10.1038/s41556-017-0016-9. Epub 2018 Jan 15. PubMed
CD163 knockout pigs are fully resistant to highly pathogenic porcine reproductive and respiratory syndrome virus. Yang H, Zhang J, Zhang X, Shi J, Pan Y, Zhou R, Li G, Li Z, Cai G, Wu Z. Antiviral Res. 2018 Mar;151:63-70. doi: 10.1016/j.antiviral.2018.01.004. Epub 2018 Jan 11. PubMed
Human Pancreatic Tumor Organoids Reveal Loss of Stem Cell Niche Factor Dependence during Disease Progression. Seino T, Kawasaki S, Shimokawa M, Tamagawa H, Toshimitsu K, Fujii M, Ohta Y, Matano M, Nanki K, Kawasaki K, Takahashi S, Sugimoto S, Iwasaki E, Takagi J, Itoi T, Kitago M, Kitagawa Y, Kanai T, Sato T. Cell Stem Cell. 2018 Mar 1;22(3):454-467.e6. doi: 10.1016/j.stem.2017.12.009. Epub 2018 Jan 11. PubMed
Critical roles of alphaII spectrin in brain development and epileptic encephalopathy. Wang Y, Ji T, Nelson AD, Glanowska K, Murphy GG, Jenkins PM, Parent JM. J Clin Invest. 2018 Feb 1;128(2):760-773. doi: 10.1172/JCI95743. Epub 2018 Jan 16. PubMed
In Vivo Ovarian Cancer Gene Therapy Using CRISPR-Cas9. He ZY, Zhang YG, Yang YH, Ma CC, Wang P, Du W, Li L, Xiang R, Song XR, Zhao X, Yao SH, Wei YQ. Hum Gene Ther. 2018 Feb;29(2):223-233. doi: 10.1089/hum.2017.209. PubMed
Negative regulation of the Keap1-Nrf2 pathway by a p62/Sqstm1 splicing variant. Kageyama S, Saito T, Obata M, Koide RH, Ichimura Y, Komatsu M. Mol Cell Biol. 2018 Jan 16. pii: MCB.00642-17. doi: 10.1128/MCB.00642-17. PubMed Associated Plasmids pGFP-p62 var2 p3xFLAG-p62 var2 p3xFLAG-p62 PB1mut p3xFLAG-p62 var2 PB1mut
CRISPR/Cas9-mediated reversibly immortalized mouse bone marrow stromal stem cells (BMSCs) retain multipotent features of mesenchymal stem cells (MSCs). Hu X, Li L, Yu X, Zhang R, Yan S, Zeng Z, Shu Y, Zhao C, Wu X, Lei J, Li Y, Zhang W, Yang C, Wu K, Wu Y, An L, Huang S, Ji X, Gong C, Yuan C, Zhang L, Liu W, Huang B, Feng Y, Zhang B, Haydon RC, Luu HH, Reid RR, Lee MJ, Wolf JM, Yu Z, He TC. Oncotarget. 2017 Dec 5;8(67):111847-111865. doi: 10.18632/oncotarget.22915. eCollection 2017 Dec 19. PubMed
Homeostatic Response of Mouse renin Gene Transcription in a Hypertensive Environment Is Mediated by a Novel 5' Enhancer. Ushiki A, Matsuzaki H, Fukamizu A, Tanimoto K. Mol Cell Biol. 2018 Mar 15;38(7). pii: MCB.00566-17. doi: 10.1128/MCB.00566-17. Print 2018 Apr 1. PubMed
SWELL1 is a glucose sensor regulating beta-cell excitability and systemic glycaemia. Kang C, Xie L, Gunasekar SK, Mishra A, Zhang Y, Pai S, Gao Y, Kumar A, Norris AW, Stephens SB, Sah R. Nat Commun. 2018 Jan 25;9(1):367. doi: 10.1038/s41467-017-02664-0. PubMed
Revolutionizing male fertility factor research in mice by using the genome editing tool CRISPR/Cas9. Abbasi F, Miyata H, Ikawa M. Reprod Med Biol. 2017 Oct 14;17(1):3-10. doi: 10.1002/rmb2.12067. eCollection 2018 Jan. PubMed
Mitochondria-specific photoactivation to monitor local sphingosine metabolism and function. Feng S, Harayama T, Montessuit S, David FP, Winssinger N, Martinou JC, Riezman H. Elife. 2018 Jan 29;7. pii: 34555. doi: 10.7554/eLife.34555. PubMed
Genome and epigenome engineering CRISPR toolkit for in vivo modulation of cis-regulatory interactions and gene expression in the chicken embryo. Williams RM, Senanayake U, Artibani M, Taylor G, Wells D, Ahmed AA, Sauka-Spengler T. Development. 2018 Feb 23;145(4). pii: dev.160333. doi: 10.1242/dev.160333. PubMed Associated Plasmids pCAG MCP-VP64 pCAG Cas9-2A-Citrine pcU6_3 sgRNA pX330a dCas9-KRAB pX330a dCas9-LSD1 More ...
Metabolic and chemical regulation of tRNA modification associated with taurine deficiency and human disease. Asano K, Suzuki T, Saito A, Wei FY, Ikeuchi Y, Numata T, Tanaka R, Yamane Y, Yamamoto T, Goto T, Kishita Y, Murayama K, Ohtake A, Okazaki Y, Tomizawa K, Sakaguchi Y, Suzuki T. Nucleic Acids Res. 2018 Feb 28;46(4):1565-1583. doi: 10.1093/nar/gky068. PubMed
R-spondins can potentiate WNT signaling without LGRs. Lebensohn AM, Rohatgi R. Elife. 2018 Feb 6;7. pii: 33126. doi: 10.7554/eLife.33126. PubMed
Reduced recruitment of 53BP1 during interstrand crosslink repair is associated with genetically inherited attenuation of mitomycin C sensitivity in a family with Fanconi anemia. Lesport E, Ferster A, Biver A, Roch B, Vasquez N, Jabado N, Vives FL, Revy P, Soulier J, de Villartay JP. Oncotarget. 2017 Dec 17;9(3):3779-3793. doi: 10.18632/oncotarget.23375. eCollection 2018 Jan 9. PubMed
Proteomic profiling of HIV-infected T-cells by SWATH mass spectrometry. DeBoer J, Wojtkiewicz MS, Haverland N, Li Y, Harwood E, Leshen E, George JW, Ciborowski P, Belshan M. Virology. 2018 Mar;516:246-257. doi: 10.1016/j.virol.2018.01.025. PubMed
RHBDD1 promotes colorectal cancer metastasis through the Wnt signaling pathway and its downstream target ZEB1. Zhang M, Miao F, Huang R, Liu W, Zhao Y, Jiao T, Lu Y, Wu F, Wang X, Wang H, Zhao H, Ju H, Miao S, Wang L, Song W. J Exp Clin Cancer Res. 2018 Feb 9;37(1):22. doi: 10.1186/s13046-018-0687-5. PubMed
CRISPR/CAS9 ablation of individual miRNAs from a miRNA family reveals their individual efficacies for regulating cardiac differentiation. Zhang Z, Ursin R, Mahapatra S, Gallicano GI. Mech Dev. 2018 Apr;150:10-20. doi: 10.1016/j.mod.2018.02.002. Epub 2018 Feb 8. PubMed
A highly specific SpCas9 variant is identified by in vivo screening in yeast. Casini A, Olivieri M, Petris G, Montagna C, Reginato G, Maule G, Lorenzin F, Prandi D, Romanel A, Demichelis F, Inga A, Cereseto A. Nat Biotechnol. 2018 Jan 29. pii: nbt.4066. doi: 10.1038/nbt.4066. PubMed Associated Plasmids pX-evoCas9
SNX18 regulates ATG9A trafficking from recycling endosomes by recruiting Dynamin-2. Soreng K, Munson MJ, Lamb CA, Bjorndal GT, Pankiv S, Carlsson SR, Tooze SA, Simonsen A. EMBO Rep. 2018 Apr;19(4). pii: embr.201744837. doi: 10.15252/embr.201744837. Epub 2018 Feb 7. PubMed
Lineage specific transcription factors and epigenetic regulators mediate TGFbeta-dependent enhancer activation. Fueyo R, Iacobucci S, Pappa S, Estaras C, Lois S, Vicioso-Mantis M, Navarro C, Cruz-Molina S, Reyes JC, Rada-Iglesias A, de la Cruz X, Martinez-Balbas MA. Nucleic Acids Res. 2018 Apr 20;46(7):3351-3365. doi: 10.1093/nar/gky093. PubMed
Heritable, Allele-Specific Chromosomal Looping between Tandem Promoters Specifies Promoter Usage of SHC1. Li X, Lin Z, Wang H, Zhao D, Xu X, Wei Y, Li X, Li X, Xiang Y, Terada LS, Liu Z. Mol Cell Biol. 2018 Apr 16;38(9). pii: MCB.00658-17. doi: 10.1128/MCB.00658-17. Print 2018 May 1. PubMed
C8ORF37 Is Required for Photoreceptor Outer Segment Disc Morphogenesis by Maintaining Outer Segment Membrane Protein Homeostasis. Sharif AS, Yu D, Loertscher S, Austin R, Nguyen K, Mathur PD, Clark AM, Zou J, Lobanova ES, Arshavsky VY, Yang J. J Neurosci. 2018 Mar 28;38(13):3160-3176. doi: 10.1523/JNEUROSCI.2964-17.2018. Epub 2018 Feb 13. PubMed
TGFbeta drives immune evasion in genetically reconstituted colon cancer metastasis. Tauriello DVF, Palomo-Ponce S, Stork D, Berenguer-Llergo A, Badia-Ramentol J, Iglesias M, Sevillano M, Ibiza S, Canellas A, Hernando-Momblona X, Byrom D, Matarin JA, Calon A, Rivas EI, Nebreda AR, Riera A, Attolini CS, Batlle E. Nature. 2018 Feb 22;554(7693):538-543. doi: 10.1038/nature25492. Epub 2018 Feb 14. PubMed
DNA mismatch repair and oligonucleotide end-protection promote base-pair substitution distal from a CRISPR/Cas9-induced DNA break. Harmsen T, Klaasen S, van de Vrugt H, Te Riele H. Nucleic Acids Res. 2018 Apr 6;46(6):2945-2955. doi: 10.1093/nar/gky076. PubMed
Pold3 is required for genomic stability and telomere integrity in embryonic stem cells and meiosis. Zhou Z, Wang L, Ge F, Gong P, Wang H, Wang F, Chen L, Liu L. Nucleic Acids Res. 2018 Apr 20;46(7):3468-3486. doi: 10.1093/nar/gky098. PubMed
HDAC1 and HDAC2 integrate checkpoint kinase phosphorylation and cell fate through the phosphatase-2A subunit PR130. Goder A, Emmerich C, Nikolova T, Kiweler N, Schreiber M, Kuhl T, Imhof D, Christmann M, Heinzel T, Schneider G, Kramer OH. Nat Commun. 2018 Feb 22;9(1):764. doi: 10.1038/s41467-018-03096-0. PubMed
BBSome trains remove activated GPCRs from cilia by enabling passage through the transition zone. Ye F, Nager AR, Nachury MV. J Cell Biol. 2018 May 7;217(5):1847-1868. doi: 10.1083/jcb.201709041. Epub 2018 Feb 26. PubMed
RNA-dependent chromatin targeting of TET2 for endogenous retrovirus control in pluripotent stem cells. Guallar D, Bi X, Pardavila JA, Huang X, Saenz C, Shi X, Zhou H, Faiola F, Ding J, Haruehanroengra P, Yang F, Li D, Sanchez-Priego C, Saunders A, Pan F, Valdes VJ, Kelley K, Blanco MG, Chen L, Wang H, Sheng J, Xu M, Fidalgo M, Shen X, Wang J. Nat Genet. 2018 Mar;50(3):443-451. doi: 10.1038/s41588-018-0060-9. Epub 2018 Feb 26. PubMed
Conserved roles of C. elegans and human MANFs in sulfatide binding and cytoprotection. Bai M, Vozdek R, Hnizda A, Jiang C, Wang B, Kuchar L, Li T, Zhang Y, Wood C, Feng L, Dang Y, Ma DK. Nat Commun. 2018 Mar 1;9(1):897. doi: 10.1038/s41467-018-03355-0. PubMed
Development of a multiple-gene-loading method by combining multi-integration system-equipped mouse artificial chromosome vector and CRISPR-Cas9. Honma K, Abe S, Endo T, Uno N, Oshimura M, Ohbayashi T, Kazuki Y. PLoS One. 2018 Mar 5;13(3):e0193642. doi: 10.1371/journal.pone.0193642. eCollection 2018. PubMed
Microhomology-assisted scarless genome editing in human iPSCs. Kim SI, Matsumoto T, Kagawa H, Nakamura M, Hirohata R, Ueno A, Ohishi M, Sakuma T, Soga T, Yamamoto T, Woltjen K. Nat Commun. 2018 Mar 5;9(1):939. doi: 10.1038/s41467-018-03044-y. PubMed Associated Plasmids CAG-Avr-HPRT-LEFT CAG-Avr-HPRT-RIGHT pX-EGFP-g1 pX-APRT-sg2 p3-HPRT1-S104R-PdTK-mCh More ...
MAP4K3 mediates amino acid-dependent regulation of autophagy via phosphorylation of TFEB. Hsu CL, Lee EX, Gordon KL, Paz EA, Shen WC, Ohnishi K, Meisenhelder J, Hunter T, La Spada AR. Nat Commun. 2018 Mar 5;9(1):942. doi: 10.1038/s41467-018-03340-7. PubMed Associated Plasmids 3918-37 3919-32 3922-48 3923-51 3924-55 More ...
Decapping protein EDC4 regulates DNA repair and phenocopies BRCA1. Hernandez G, Ramirez MJ, Minguillon J, Quiles P, Ruiz de Garibay G, Aza-Carmona M, Bogliolo M, Pujol R, Prados-Carvajal R, Fernandez J, Garcia N, Lopez A, Gutierrez-Enriquez S, Diez O, Benitez J, Salinas M, Teule A, Brunet J, Radice P, Peterlongo P, Schindler D, Huertas P, Puente XS, Lazaro C, Pujana MA, Surralles J. Nat Commun. 2018 Mar 6;9(1):967. doi: 10.1038/s41467-018-03433-3. PubMed
CRISPR-Cas9-Based Genome Editing of Human Induced Pluripotent Stem Cells. Giacalone JC, Sharma TP, Burnight ER, Fingert JF, Mullins RF, Stone EM, Tucker BA. Curr Protoc Stem Cell Biol. 2018 Feb 28;44:5B.7.1-5B.7.22. doi: 10.1002/cpsc.46. PubMed Associated Plasmids pX330GFP-hU6-gRNA-hSpCas9
Epigenetic reprogramming enables the transition from primordial germ cell to gonocyte. Hill PWS, Leitch HG, Requena CE, Sun Z, Amouroux R, Roman-Trufero M, Borkowska M, Terragni J, Vaisvila R, Linnett S, Bagci H, Dharmalingham G, Haberle V, Lenhard B, Zheng Y, Pradhan S, Hajkova P. Nature. 2018 Mar 15;555(7696):392-396. doi: 10.1038/nature25964. Epub 2018 Mar 7. PubMed
Screening Regulatory Element Function with CRISPR/Cas9-based Epigenome Editing. Klann TS, Crawford GE, Reddy TE, Gersbach CA. Methods Mol Biol. 2018;1767:447-480. doi: 10.1007/978-1-4939-7774-1_25. PubMed
PKM1 Confers Metabolic Advantages and Promotes Cell-Autonomous Tumor Cell Growth. Morita M, Sato T, Nomura M, Sakamoto Y, Inoue Y, Tanaka R, Ito S, Kurosawa K, Yamaguchi K, Sugiura Y, Takizaki H, Yamashita Y, Katakura R, Sato I, Kawai M, Okada Y, Watanabe H, Kondoh G, Matsumoto S, Kishimoto A, Obata M, Matsumoto M, Fukuhara T, Motohashi H, Suematsu M, Komatsu M, Nakayama KI, Watanabe T, Soga T, Shima H, Maemondo M, Tanuma N. Cancer Cell. 2018 Mar 12;33(3):355-367.e7. doi: 10.1016/j.ccell.2018.02.004. PubMed
Easy quantification of template-directed CRISPR/Cas9 editing. Brinkman EK, Kousholt AN, Harmsen T, Leemans C, Chen T, Jonkers J, van Steensel B. Nucleic Acids Res. 2018 Jun 1;46(10):e58. doi: 10.1093/nar/gky164. PubMed
Intravesicular Acidification Regulates Lipopolysaccharide Inflammation and Tolerance through TLR4 Trafficking. Murase M, Kawasaki T, Hakozaki R, Sueyoshi T, Putri DDP, Kitai Y, Sato S, Ikawa M, Kawai T. J Immunol. 2018 Apr 15;200(8):2798-2808. doi: 10.4049/jimmunol.1701390. Epub 2018 Mar 14. PubMed
Pinpointing recurrent proviral integration sites in new models for latent HIV-1 infection. Lange UC, Bialek JK, Walther T, Hauber J. Virus Res. 2018 Apr 2;249:69-75. doi: 10.1016/j.virusres.2018.03.007. Epub 2018 Mar 14. PubMed
Synapse maintenance and restoration in the retina by NGL2. Soto F, Zhao L, Kerschensteiner D. Elife. 2018 Mar 19;7. pii: 30388. doi: 10.7554/eLife.30388. PubMed
Adjuvant Treatment for POLE Proofreading Domain-Mutant Cancers: Sensitivity to Radiotherapy, Chemotherapy, and Nucleoside Analogues. Van Gool IC, Rayner E, Osse EM, Nout RA, Creutzberg CL, Tomlinson IPM, Church DN, Smit VTHBM, de Wind N, Bosse T, Drost M. Clin Cancer Res. 2018 Jul 1;24(13):3197-3203. doi: 10.1158/1078-0432.CCR-18-0266. Epub 2018 Mar 20. PubMed
Neuropathic pain in a Fabry disease rat model. Miller JJ, Aoki K, Moehring F, Murphy CA, O'Hara CL, Tiemeyer M, Stucky CL, Dahms NM. JCI Insight. 2018 Mar 22;3(6). pii: 99171. doi: 10.1172/jci.insight.99171. PubMed
Role for Wnt Signaling in Retinal Neuropil Development: Analysis via RNA-Seq and In Vivo Somatic CRISPR Mutagenesis. Sarin S, Zuniga-Sanchez E, Kurmangaliyev YZ, Cousins H, Patel M, Hernandez J, Zhang KX, Samuel MA, Morey M, Sanes JR, Zipursky SL. Neuron. 2018 Mar 22. pii: S0896-6273(18)30181-8. doi: 10.1016/j.neuron.2018.03.004. PubMed
CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy. Yao X, Wang X, Liu J, Shi L, Huang P, Yang H. J Vis Exp. 2018 Mar 12;(133). doi: 10.3791/56844. PubMed
Rescue of an aggressive female sexual courtship in mice by CRISPR/Cas9 secondary mutation in vivo. Zakany J, Duboule D. BMC Res Notes. 2018 Mar 27;11(1):193. doi: 10.1186/s13104-018-3307-8. PubMed
Paired D10A Cas9 nickases are sometimes more efficient than individual nucleases for gene disruption. Gopalappa R, Suresh B, Ramakrishna S, Kim HH. Nucleic Acids Res. 2018 Jul 6;46(12):e71. doi: 10.1093/nar/gky222. PubMed
An E2F7-dependent transcriptional program modulates DNA damage repair and genomic stability. Mitxelena J, Apraiz A, Vallejo-Rodriguez J, Garcia-Santisteban I, Fullaondo A, Alvarez-Fernandez M, Malumbres M, Zubiaga AM. Nucleic Acids Res. 2018 May 18;46(9):4546-4559. doi: 10.1093/nar/gky218. PubMed
MLL5 suppresses antiviral innate immune response by facilitating STUB1-mediated RIG-I degradation. Zhou P, Ding X, Wan X, Liu L, Yuan X, Zhang W, Hui X, Meng G, Xiao H, Li B, Zhong J, Hou F, Deng L, Zhang Y. Nat Commun. 2018 Mar 28;9(1):1243. doi: 10.1038/s41467-018-03563-8. PubMed
In Vivo Genome Editing Partially Restores Alpha1-Antitrypsin in a Murine Model of AAT Deficiency. Song CQ, Wang D, Jiang T, O'Connor K, Tang Q, Cai L, Li X, Weng Z, Yin H, Gao G, Mueller C, Flotte TR, Xue W. Hum Gene Ther. 2018 Aug;29(8):853-860. doi: 10.1089/hum.2017.225. Epub 2018 May 14. PubMed
A Transcriptional Circuit Filters Oscillating Circadian Hormonal Inputs to Regulate Fat Cell Differentiation. Bahrami-Nejad Z, Zhao ML, Tholen S, Hunerdosse D, Tkach KE, van Schie S, Chung M, Teruel MN. Cell Metab. 2018 Apr 3;27(4):854-868.e8. doi: 10.1016/j.cmet.2018.03.012. PubMed
A multi-landing pad DNA integration platform for mammalian cell engineering. Gaidukov L, Wroblewska L, Teague B, Nelson T, Zhang X, Liu Y, Jagtap K, Mamo S, Tseng WA, Lowe A, Das J, Bandara K, Baijuraj S, Summers NM, Lu TK, Zhang L, Weiss R. Nucleic Acids Res. 2018 May 4;46(8):4072-4086. doi: 10.1093/nar/gky216. PubMed
Increasing antigen presentation on HSV-1-infected cells increases lesion size but does not alter neural infection or latency. Russell TA, Velusamy T, Tseng YY, Tscharke DC. J Gen Virol. 2018 May;99(5):682-692. doi: 10.1099/jgv.0.001059. Epub 2018 Apr 5. PubMed
Carbonic anhydrase IX is a critical determinant of pulmonary microvascular endothelial cell pH regulation and angiogenesis during acidosis. Lee JY, Alexeyev M, Kozhukhar N, Pastukh V, White R, Stevens T. Am J Physiol Lung Cell Mol Physiol. 2018 Jul 1;315(1):L41-L51. doi: 10.1152/ajplung.00446.2017. Epub 2018 Apr 5. PubMed
GDF15 promotes the proliferation of cervical cancer cells by phosphorylating AKT1 and Erk1/2 through the receptor ErbB2. Li S, Ma YM, Zheng PS, Zhang P. J Exp Clin Cancer Res. 2018 Apr 10;37(1):80. doi: 10.1186/s13046-018-0744-0. PubMed
Repeat-mediated deletions can be induced by a chromosomal break far from a repeat, but multiple pathways suppress such rearrangements. Mendez-Dorantes C, Bhargava R, Stark JM. Genes Dev. 2018 Apr 1;32(7-8):524-536. doi: 10.1101/gad.311084.117. Epub 2018 Apr 10. PubMed
Transcriptional Dysregulation of MYC Reveals Common Enhancer-Docking Mechanism. Schuijers J, Manteiga JC, Weintraub AS, Day DS, Zamudio AV, Hnisz D, Lee TI, Young RA. Cell Rep. 2018 Apr 10;23(2):349-360. doi: 10.1016/j.celrep.2018.03.056. PubMed
CRISPR/Cas9 Inhibits Multiple Steps of HIV-1 Infection. Yin L, Hu S, Mei S, Sun H, Xu F, Li J, Zhu W, Liu X, Zhao F, Zhang D, Cen S, Liang C, Guo F. Hum Gene Ther. 2018 Nov;29(11):1264-1276. doi: 10.1089/hum.2018.018. Epub 2018 May 9. PubMed
Suppressing Ku70/Ku80 expression elevates homology-directed repair efficiency in primary fibroblasts. Li G, Liu D, Zhang X, Quan R, Zhong C, Mo J, Huang Y, Wang H, Ruan X, Xu Z, Zheng E, Gu T, Hong L, Li Z, Wu Z, Yang H. Int J Biochem Cell Biol. 2018 Jun;99:154-160. doi: 10.1016/j.biocel.2018.04.011. Epub 2018 Apr 12. PubMed
Crystal structure of intraflagellar transport protein 80 reveals a homo-dimer required for ciliogenesis. Taschner M, Lorentzen A, Mourao A, Collins T, Freke GM, Moulding D, Basquin J, Jenkins D, Lorentzen E. Elife. 2018 Apr 16;7. pii: 33067. doi: 10.7554/eLife.33067. PubMed
Conserved and divergent functions of Pax6 underlie species-specific neurogenic patterns in the developing amniote brain. Yamashita W, Takahashi M, Kikkawa T, Gotoh H, Osumi N, Ono K, Nomura T. Development. 2018 Apr 16;145(8). pii: 145/8/dev159764. doi: 10.1242/dev.159764. PubMed
Comparative Analysis of UL16 Mutants Derived from Multiple Strains of Herpes Simplex Virus 2 (HSV-2) and HSV-1 Reveals Species-Specific Requirements for the UL16 Protein. Gao J, Yan X, Banfield BW. J Virol. 2018 Jun 13;92(13). pii: JVI.00629-18. doi: 10.1128/JVI.00629-18. Print 2018 Jul 1. PubMed
Physiological function of phospholipase D2 in anti-tumor immunity: regulation of CD8(+) T lymphocyte proliferation. Ngo Thai Bich V, Hongu T, Miura Y, Katagiri N, Ohbayashi N, Yamashita-Kanemaru Y, Shibuya A, Funakoshi Y, Kanaho Y. Sci Rep. 2018 Apr 19;8(1):6283. doi: 10.1038/s41598-018-24512-x. PubMed
KREMEN1 Is a Host Entry Receptor for a Major Group of Enteroviruses. Staring J, van den Hengel LG, Raaben M, Blomen VA, Carette JE, Brummelkamp TR. Cell Host Microbe. 2018 May 9;23(5):636-643.e5. doi: 10.1016/j.chom.2018.03.019. Epub 2018 Apr 19. PubMed
Loss of tafazzin results in decreased myoblast differentiation in C2C12 cells: A myoblast model of Barth syndrome and cardiolipin deficiency. Lou W, Reynolds CA, Li Y, Liu J, Huttemann M, Schlame M, Stevenson D, Strathdee D, Greenberg ML. Biochim Biophys Acta Mol Cell Biol Lipids. 2018 Aug;1863(8):857-865. doi: 10.1016/j.bbalip.2018.04.015. Epub 2018 Apr 22. PubMed
Role of Complement in a Rat Model of Paclitaxel-Induced Peripheral Neuropathy. Xu J, Zhang L, Xie M, Li Y, Huang P, Saunders TL, Fox DA, Rosenquist R, Lin F. J Immunol. 2018 Jun 15;200(12):4094-4101. doi: 10.4049/jimmunol.1701716. Epub 2018 Apr 25. PubMed
Redundant and Antagonistic Roles of XTP3B and OS9 in Decoding Glycan and Non-glycan Degrons in ER-Associated Degradation. van der Goot AT, Pearce MMP, Leto DE, Shaler TA, Kopito RR. Mol Cell. 2018 May 3;70(3):516-530.e6. doi: 10.1016/j.molcel.2018.03.026. Epub 2018 Apr 26. PubMed
Mental Illnesses-Associated Fxr1 and Its Negative Regulator Gsk3beta Are Modulators of Anxiety and Glutamatergic Neurotransmission. Khlghatyan J, Evstratova A, Chamberland S, Marakhovskaia A, Bahremand A, Toth K, Beaulieu JM. Front Mol Neurosci. 2018 Apr 12;11:119. doi: 10.3389/fnmol.2018.00119. eCollection 2018. PubMed Associated Plasmids pAAV hSyn GFP-Fxr1 pAAV Gsk3 sgRNA/GFP
Complex formation between the vasopressin 1b receptor, beta-arrestin-2, and the mu-opioid receptor underlies morphine tolerance. Koshimizu TA, Honda K, Nagaoka-Uozumi S, Ichimura A, Kimura I, Nakaya M, Sakai N, Shibata K, Ushijima K, Fujimura A, Hirasawa A, Kurose H, Tsujimoto G, Tanoue A, Takano Y. Nat Neurosci. 2018 Jun;21(6):820-833. doi: 10.1038/s41593-018-0144-y. Epub 2018 Apr 30. PubMed
The BUB3-BUB1 Complex Promotes Telomere DNA Replication. Li F, Kim H, Ji Z, Zhang T, Chen B, Ge Y, Hu Y, Feng X, Han X, Xu H, Zhang Y, Yu H, Liu D, Ma W, Songyang Z. Mol Cell. 2018 May 3;70(3):395-407.e4. doi: 10.1016/j.molcel.2018.03.032. PubMed
Generation of App knock-in mice reveals deletion mutations protective against Alzheimer's disease-like pathology. Nagata K, Takahashi M, Matsuba Y, Okuyama-Uchimura F, Sato K, Hashimoto S, Saito T, Saido TC. Nat Commun. 2018 May 4;9(1):1800. doi: 10.1038/s41467-018-04238-0. PubMed
Tissue Distribution of Kir7.1 Inwardly Rectifying K(+) Channel Probed in a Knock-in Mouse Expressing a Haemagglutinin-Tagged Protein. Cornejo I, Villanueva S, Burgos J, Lopez-Cayuqueo KI, Chambrey R, Julio-Kalajzic F, Buelvas N, Niemeyer MI, Figueiras-Fierro D, Brown PD, Sepulveda FV, Cid LP. Front Physiol. 2018 Apr 23;9:428. doi: 10.3389/fphys.2018.00428. eCollection 2018. PubMed
CD155 loss enhances tumor suppression via combined host and tumor-intrinsic mechanisms. Li XY, Das I, Lepletier A, Addala V, Bald T, Stannard K, Barkauskas D, Liu J, Aguilera AR, Takeda K, Braun M, Nakamura K, Jacquelin S, Lane SW, Teng MW, Dougall WC, Smyth MJ. J Clin Invest. 2018 Jun 1;128(6):2613-2625. doi: 10.1172/JCI98769. Epub 2018 May 14. PubMed
CO2-sensitive tRNA modification associated with human mitochondrial disease. Lin H, Miyauchi K, Harada T, Okita R, Takeshita E, Komaki H, Fujioka K, Yagasaki H, Goto YI, Yanaka K, Nakagawa S, Sakaguchi Y, Suzuki T. Nat Commun. 2018 May 14;9(1):1875. doi: 10.1038/s41467-018-04250-4. PubMed
CRISPR/Cas9 Mutagenesis of UL21 in Multiple Strains of Herpes Simplex Virus Reveals Differential Requirements for pUL21 in Viral Replication. Finnen RL, Banfield BW. Viruses. 2018 May 15;10(5). pii: v10050258. doi: 10.3390/v10050258. PubMed
A Critical Neurodevelopmental Role for L-Type Voltage-Gated Calcium Channels in Neurite Extension and Radial Migration. Kamijo S, Ishii Y, Horigane SI, Suzuki K, Ohkura M, Nakai J, Fujii H, Takemoto-Kimura S, Bito H. J Neurosci. 2018 Jun 13;38(24):5551-5566. doi: 10.1523/JNEUROSCI.2357-17.2018. Epub 2018 May 17. PubMed
Plasmodium parasite exploits host aquaporin-3 during liver stage malaria infection. Posfai D, Sylvester K, Reddy A, Ganley JG, Wirth J, Cullen QE, Dave T, Kato N, Dave SS, Derbyshire ER. PLoS Pathog. 2018 May 18;14(5):e1007057. doi: 10.1371/journal.ppat.1007057. eCollection 2018 May. PubMed
Derivation and characterization of a UCP1 reporter human ES cell line. Mukherjee S, Zhang T, Lacko LA, Tan L, Xiang JZ, Butler JM, Chen S. Stem Cell Res. 2018 Jul;30:12-21. doi: 10.1016/j.scr.2018.04.007. Epub 2018 Apr 22. PubMed
Tild-CRISPR Allows for Efficient and Precise Gene Knockin in Mouse and Human Cells. Yao X, Zhang M, Wang X, Ying W, Hu X, Dai P, Meng F, Shi L, Sun Y, Yao N, Zhong W, Li Y, Wu K, Li W, Chen ZJ, Yang H. Dev Cell. 2018 May 21;45(4):526-536.e5. doi: 10.1016/j.devcel.2018.04.021. PubMed
In vivo gene correction with targeted sequence substitution through microhomology-mediated end joining. Shin JH, Jung S, Ramakrishna S, Kim HH, Lee J. Biochem Biophys Res Commun. 2018 Jul 7;502(1):116-122. doi: 10.1016/j.bbrc.2018.05.130. Epub 2018 May 24. PubMed
Cell surface flip-flop of phosphatidylserine is critical for PIEZO1-mediated myotube formation. Tsuchiya M, Hara Y, Okuda M, Itoh K, Nishioka R, Shiomi A, Nagao K, Mori M, Mori Y, Ikenouchi J, Suzuki R, Tanaka M, Ohwada T, Aoki J, Kanagawa M, Toda T, Nagata Y, Matsuda R, Takayama Y, Tominaga M, Umeda M. Nat Commun. 2018 May 24;9(1):2049. doi: 10.1038/s41467-018-04436-w. PubMed
delete Lambrus BG, Moyer TC, Holland AJ. 2018 PubMed
Kinetics and Fidelity of the Repair of Cas9-Induced Double-Strand DNA Breaks. Brinkman EK, Chen T, de Haas M, Holland HA, Akhtar W, van Steensel B. Mol Cell. 2018 Jun 7;70(5):801-813.e6. doi: 10.1016/j.molcel.2018.04.016. Epub 2018 May 24. PubMed
Dynein-Dynactin-NuMA clusters generate cortical spindle-pulling forces as a multi-arm ensemble. Okumura M, Natsume T, Kanemaki MT, Kiyomitsu T. Elife. 2018 May 31;7. pii: 36559. doi: 10.7554/eLife.36559. PubMed Associated Plasmids pMK231 (AAVS1 CMV-MCS-PURO) hROSA26 CRISPR-pX330 pTK511 pTK372 pTK421 More ...
Combinatorial knockout of RARalpha, RARbeta, and RARgamma completely abrogates transcriptional responses to retinoic acid in murine embryonic stem cells. Laursen KB, Gudas LJ. J Biol Chem. 2018 Jul 27;293(30):11891-11900. doi: 10.1074/jbc.RA118.001951. Epub 2018 May 30. PubMed
Amino Acid Variation at VP1-145 of Enterovirus 71 Determines Attachment Receptor Usage and Neurovirulence in Human Scavenger Receptor B2 Transgenic Mice. Kobayashi K, Sudaka Y, Takashino A, Imura A, Fujii K, Koike S. J Virol. 2018 Jul 17;92(15). pii: JVI.00681-18. doi: 10.1128/JVI.00681-18. Print 2018 Aug 1. PubMed
CRISPR/Cas9-mediated gene knockout for DNA methyltransferase Dnmt3a in CHO cells displays enhanced transgenic expression and long-term stability. Jia YL, Guo X, Lu JT, Wang XY, Qiu LL, Wang TY. J Cell Mol Med. 2018 Sep;22(9):4106-4116. doi: 10.1111/jcmm.13687. Epub 2018 May 30. PubMed
Efficient genome editing by FACS enrichment of paired D10A Cas9 nickases coupled with fluorescent proteins. Gopalappa R, Song M, Chandrasekaran AP, Das S, Haq S, Koh HC, Ramakrishna S. Arch Pharm Res. 2018 Sep;41(9):911-920. doi: 10.1007/s12272-018-1042-2. Epub 2018 May 31. PubMed
CRISPR/Cas9-Mediated Genome Editing for Huntington's Disease. Vachey G, Deglon N. Methods Mol Biol. 2018;1780:463-481. doi: 10.1007/978-1-4939-7825-0_21. PubMed
Insights into the mechanisms underlying the inactivation of HIV-1 proviruses by CRISPR/Cas. Mefferd AL, Bogerd HP, Irwan ID, Cullen BR. Virology. 2018 Jul;520:116-126. doi: 10.1016/j.virol.2018.05.016. Epub 2018 May 29. PubMed
Generation of Hutat2:Fc Knockin Primary Human Monocytes Using CRISPR/Cas9. Wang B, Zuo J, Kang W, Wei Q, Li J, Wang C, Liu Z, Lu Y, Zhuang Y, Dang B, Liu Q, Kang W, Sun Y. Mol Ther Nucleic Acids. 2018 Jun 1;11:130-141. doi: 10.1016/j.omtn.2018.01.012. Epub 2018 Feb 7. PubMed
Depleting Trim28 in adult mice is well tolerated and reduces levels of alpha-synuclein and tau. Rousseaux MW, Revelli JP, Vazquez-Velez GE, Kim JY, Craigen E, Gonzales K, Beckinghausen J, Zoghbi HY. Elife. 2018 Jun 4;7. pii: 36768. doi: 10.7554/eLife.36768. PubMed
Global untargeted serum metabolomic analyses nominate metabolic pathways responsive to loss of expression of the orphan metallo beta-lactamase, MBLAC1. Gibson CL, Codreanu SG, Schrimpe-Rutledge AC, Retzlaff CL, Wright J, Mortlock DP, Sherrod SD, McLean JA, Blakely RD. Mol Omics. 2018 Jun 12;14(3):142-155. doi: 10.1039/c7mo00022g. PubMed
Biallelic UFM1 and UFC1 mutations expand the essential role of ufmylation in brain development. Nahorski MS, Maddirevula S, Ishimura R, Alsahli S, Brady AF, Begemann A, Mizushima T, Guzman-Vega FJ, Obata M, Ichimura Y, Alsaif HS, Anazi S, Ibrahim N, Abdulwahab F, Hashem M, Monies D, Abouelhoda M, Meyer BF, Alfadhel M, Eyaid W, Zweier M, Steindl K, Rauch A, Arold ST, Woods CG, Komatsu M, Alkuraya FS. Brain. 2018 Jul 1;141(7):1934-1945. doi: 10.1093/brain/awy135. PubMed
MICMIC: identification of DNA methylation of distal regulatory regions with causal effects on tumorigenesis. Tong Y, Sun J, Wong CF, Kang Q, Ru B, Wong CN, Chan AS, Leung SY, Zhang J. Genome Biol. 2018 Jun 5;19(1):73. doi: 10.1186/s13059-018-1442-0. PubMed
Diminished nuclear RNA decay upon Salmonella infection upregulates antibacterial noncoding RNAs. Imamura K, Takaya A, Ishida YI, Fukuoka Y, Taya T, Nakaki R, Kakeda M, Imamachi N, Sato A, Yamada T, Onoguchi-Mizutani R, Akizuki G, Tanu T, Tao K, Miyao S, Suzuki Y, Nagahama M, Yamamoto T, Jensen TH, Akimitsu N. EMBO J. 2018 Jul 2;37(13). pii: embj.201797723. doi: 10.15252/embj.201797723. Epub 2018 Jun 7. PubMed
Rossant lab unpublished plasmids Rossant J. Unpublished plasmids PubMed
iTAP, a novel iRhom interactor, controls TNF secretion by policing the stability of iRhom/TACE. Oikonomidi I, Burbridge E, Cavadas M, Sullivan G, Collis B, Naegele H, Clancy D, Brezinova J, Hu T, Bileck A, Gerner C, Bolado A, von Kriegsheim A, Martin SJ, Steinberg F, Strisovsky K, Adrain C. Elife. 2018 Jun 13;7. pii: 35032. doi: 10.7554/eLife.35032. PubMed
Disruption of a -35 kb Enhancer Impairs CTCF Binding and MLH1 Expression in Colorectal Cells. Liu Q, Thoms JAI, Nunez AC, Huang Y, Knezevic K, Packham D, Poulos RC, Williams R, Beck D, Hawkins NJ, Ward RL, Wong JWH, Hesson LB, Sloane MA, Pimanda JE. Clin Cancer Res. 2018 Sep 15;24(18):4602-4611. doi: 10.1158/1078-0432.CCR-17-3678. Epub 2018 Jun 13. PubMed
A Kaposi's Sarcoma-Associated Herpesvirus Infection Mechanism Is Independent of Integrins alpha3beta1, alphaVbeta3, and alphaVbeta5. TerBush AA, Hafkamp F, Lee HJ, Coscoy L. J Virol. 2018 Aug 16;92(17). pii: JVI.00803-18. doi: 10.1128/JVI.00803-18. Print 2018 Sep 1. PubMed
Efficient Knock-in of a Point Mutation in Porcine Fibroblasts Using the CRISPR/Cas9-GMNN Fusion Gene. Gerlach M, Kraft T, Brenner B, Petersen B, Niemann H, Montag J. Genes (Basel). 2018 Jun 13;9(6). pii: genes9060296. doi: 10.3390/genes9060296. PubMed
Flaviviruses Exploit the Lipid Droplet Protein AUP1 to Trigger Lipophagy and Drive Virus Production. Zhang J, Lan Y, Li MY, Lamers MM, Fusade-Boyer M, Klemm E, Thiele C, Ashour J, Sanyal S. Cell Host Microbe. 2018 Jun 13;23(6):819-831.e5. doi: 10.1016/j.chom.2018.05.005. PubMed
The chemerin knockout rat reveals chemerin dependence in female, but not male, experimental hypertension. Watts SW, Darios ES, Mullick AE, Garver H, Saunders TL, Hughes ED, Filipiak WE, Zeidler MG, McMullen N, Sinal CJ, Kumar RK, Ferland DJ, Fink GD. FASEB J. 2018 Jun 15:fj201800479. doi: 10.1096/fj.201800479. PubMed
Foot-and-mouth disease virus nonstructural protein 2B interacts with cyclophilin A, modulating virus replication. Liu H, Xue Q, Cao W, Yang F, Ma L, Liu W, Zhang K, Liu X, Zhu Z, Zheng H. FASEB J. 2018 Jun 15:fj201701351. doi: 10.1096/fj.201701351. PubMed
Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive Pluripotency. Adachi K, Kopp W, Wu G, Heising S, Greber B, Stehling M, Arauzo-Bravo MJ, Boerno ST, Timmermann B, Vingron M, Scholer HR. Cell Stem Cell. 2018 Aug 2;23(2):266-275.e6. doi: 10.1016/j.stem.2018.05.020. Epub 2018 Jun 14. PubMed Associated Plasmids KA0637_pPBCAG-rtTAM2-IN KA1775_pPBCAG-rtTAM2-IH KA0717_pPB-hCMV*1-cHA-IRESVenus KA1063_pPB-hCMV*1-cHA-IRESH2BmKO2 KA1065_pPB-hCMV*1-cHA-IRESH2BmCherry More ...
Exploiting CRISPR-Cas9 technology to investigate individual histone modifications. Vasquez JJ, Wedel C, Cosentino RO, Siegel TN. Nucleic Acids Res. 2018 Oct 12;46(18):e106. doi: 10.1093/nar/gky517. PubMed
ZMYND10 functions in a chaperone relay during axonemal dynein assembly. Mali GR, Yeyati PL, Mizuno S, Dodd DO, Tennant PA, Keighren MA, Zur Lage P, Shoemark A, Garcia-Munoz A, Shimada A, Takeda H, Edlich F, Takahashi S, von Kreigsheim A, Jarman AP, Mill P. Elife. 2018 Jun 19;7. pii: 34389. doi: 10.7554/eLife.34389. PubMed
Investigation of the role of GBF1 in the replication of positive-sense single-stranded RNA viruses. Ferlin J, Farhat R, Belouzard S, Cocquerel L, Bertin A, Hober D, Dubuisson J, Rouille Y. J Gen Virol. 2018 Aug;99(8):1086-1096. doi: 10.1099/jgv.0.001099. Epub 2018 Jun 20. PubMed
Novel lymphoid enhancer-binding factor 1-cytoglobin axis promotes extravasation of osteosarcoma cells into the lungs. Pongsuchart M, Kuchimaru T, Yonezawa S, Tran DTP, Kha NT, Hoang NTH, Kadonosono T, Kizaka-Kondoh S. Cancer Sci. 2018 Sep;109(9):2746-2756. doi: 10.1111/cas.13702. Epub 2018 Jul 24. PubMed
Flap endonuclease 1 is involved in cccDNA formation in the hepatitis B virus. Kitamura K, Que L, Shimadu M, Koura M, Ishihara Y, Wakae K, Nakamura T, Watashi K, Wakita T, Muramatsu M. PLoS Pathog. 2018 Jun 21;14(6):e1007124. doi: 10.1371/journal.ppat.1007124. eCollection 2018 Jun. PubMed
CRISPR/Cas and recombinase-based human-to-pig orthotopic gene exchange for xenotransplantation. Nunes Dos Santos RM, Carneiro D'Albuquerque LA, Reyes LM, Estrada JL, Wang ZY, Tector M, Tector AJ. J Surg Res. 2018 Sep;229:28-40. doi: 10.1016/j.jss.2018.03.051. Epub 2018 Apr 16. PubMed
CRISPR-mediated Loss of Function Analysis in Cerebellar Granule Cells Using In Utero Electroporation-based Gene Transfer. Feng W, Herbst L, Lichter P, Pfister SM, Liu HK, Kawauchi D. J Vis Exp. 2018 Jun 9;(136). doi: 10.3791/57311. PubMed
Efficient derivation of extended pluripotent stem cells from NOD-scid Il2rg(-/-) mice. Du Y, Wang T, Xu J, Zhao C, Li H, Fu Y, Xu Y, Xie L, Zhao J, Yang W, Yin M, Wen J, Deng H. Protein Cell. 2019 Jan;10(1):31-42. doi: 10.1007/s13238-018-0558-z. Epub 2018 Jun 13. PubMed
Rapid generation of gene-targeted EPS-derived mouse models through tetraploid complementation. Li H, Zhao C, Xu J, Xu Y, Cheng C, Liu Y, Wang T, Du Y, Xie L, Zhao J, Han Y, Wang X, Bai Y, Deng H. Protein Cell. 2019 Jan;10(1):20-30. doi: 10.1007/s13238-018-0556-1. Epub 2018 Jun 13. PubMed
Mitoregulin: A lncRNA-Encoded Microprotein that Supports Mitochondrial Supercomplexes and Respiratory Efficiency. Stein CS, Jadiya P, Zhang X, McLendon JM, Abouassaly GM, Witmer NH, Anderson EJ, Elrod JW, Boudreau RL. Cell Rep. 2018 Jun 26;23(13):3710-3720.e8. doi: 10.1016/j.celrep.2018.06.002. PubMed
C-NHEJ without indels is robust and requires synergistic function of distinct XLF domains. Bhargava R, Sandhu M, Muk S, Lee G, Vaidehi N, Stark JM. Nat Commun. 2018 Jun 27;9(1):2484. doi: 10.1038/s41467-018-04867-5. PubMed Associated Plasmids pCMV6-AC EJ7-GFP pCMV6-AC 4-μHOM 7a sgRNA for EJ7-GFP and 4-μHOM reporters 7b sgRNA for EJ7-GFP reporter 7h sgRNA for 4-μHOM reporter More ...
The polycomb group protein Yaf2 regulates the pluripotency of embryonic stem cells in a phosphorylation-dependent manner. Zhao W, Liu M, Ji H, Zhu Y, Wang C, Huang Y, Ma X, Xing G, Xia Y, Jiang Q, Qin J. J Biol Chem. 2018 Aug 17;293(33):12793-12804. doi: 10.1074/jbc.RA118.003299. Epub 2018 Jun 29. PubMed
Generation of the Adenovirus Vector-Mediated CRISPR/Cpf1 System and the Application for Primary Human Hepatocytes Prepared from Humanized Mice with Chimeric Liver. Tsukamoto T, Sakai E, Iizuka S, Taracena-Gandara M, Sakurai F, Mizuguchi H. Biol Pharm Bull. 2018;41(7):1089-1095. doi: 10.1248/bpb.b18-00222. PubMed
Near infrared fluorescent peptide nanoparticles for enhancing esophageal cancer therapeutic efficacy. Fan Z, Chang Y, Cui C, Sun L, Wang DH, Pan Z, Zhang M. Nat Commun. 2018 Jul 4;9(1):2605. doi: 10.1038/s41467-018-04763-y. PubMed
Prolactin signaling drives tumorigenesis in human high grade serous ovarian cancer cells and in a spontaneous fallopian tube derived model. Karthikeyan S, Russo A, Dean M, Lantvit DD, Endsley M, Burdette JE. Cancer Lett. 2018 Oct 1;433:221-231. doi: 10.1016/j.canlet.2018.07.003. Epub 2018 Jul 5. PubMed
Mettl1/Wdr4-Mediated m(7)G tRNA Methylome Is Required for Normal mRNA Translation and Embryonic Stem Cell Self-Renewal and Differentiation. Lin S, Liu Q, Lelyveld VS, Choe J, Szostak JW, Gregory RI. Mol Cell. 2018 Jul 19;71(2):244-255.e5. doi: 10.1016/j.molcel.2018.06.001. Epub 2018 Jul 5. PubMed
The N Termini of TAR DNA-Binding Protein 43 (TDP43) C-Terminal Fragments Influence Degradation, Aggregation Propensity, and Morphology. Kasu YAT, Alemu S, Lamari A, Loew N, Brower CS. Mol Cell Biol. 2018 Sep 14;38(19). pii: MCB.00243-18. doi: 10.1128/MCB.00243-18. Print 2018 Oct 1. PubMed
Oncogenic addiction to high 26S proteasome level. Tsvetkov P, Adler J, Myers N, Biran A, Reuven N, Shaul Y. Cell Death Dis. 2018 Jul 10;9(7):773. doi: 10.1038/s41419-018-0806-4. PubMed
Discovery of a drug candidate for GLIS3-associated diabetes. Amin S, Cook B, Zhou T, Ghazizadeh Z, Lis R, Zhang T, Khalaj M, Crespo M, Perera M, Xiang JZ, Zhu Z, Tomishima M, Liu C, Naji A, Evans T, Huangfu D, Chen S. Nat Commun. 2018 Jul 11;9(1):2681. doi: 10.1038/s41467-018-04918-x. PubMed
Optimized Plasmid Construction Strategy for Cas9. Xu J, Li W, Hossen MM, Jia Y, Li L, Huang Z. Cell Physiol Biochem. 2018;48(1):131-137. doi: 10.1159/000491669. Epub 2018 Jul 12. PubMed
gamma-Secretase promotes membrane insertion of the human papillomavirus L2 capsid protein during virus infection. Inoue T, Zhang P, Zhang W, Goodner-Bingham K, Dupzyk A, DiMaio D, Tsai B. J Cell Biol. 2018 Oct 1;217(10):3545-3559. doi: 10.1083/jcb.201804171. Epub 2018 Jul 13. PubMed
CRISPR/Cas9-mediated homologous recombination in tobacco. Hirohata A, Sato I, Kaino K, Iwata Y, Koizumi N, Mishiba KI. Plant Cell Rep. 2018 Jul 13. pii: 10.1007/s00299-018-2320-7. doi: 10.1007/s00299-018-2320-7. PubMed
A Suite of Transgenic Driver and Reporter Mouse Lines with Enhanced Brain-Cell-Type Targeting and Functionality. Daigle TL, Madisen L, Hage TA, Valley MT, Knoblich U, Larsen RS, Takeno MM, Huang L, Gu H, Larsen R, Mills M, Bosma-Moody A, Siverts LA, Walker M, Graybuck LT, Yao Z, Fong O, Nguyen TN, Garren E, Lenz GH, Chavarha M, Pendergraft J, Harrington J, Hirokawa KE, Harris JA, Nicovich PR, McGraw MJ, Ollerenshaw DR, Smith KA, Baker CA, Ting JT, Sunkin SM, Lecoq J, Lin MZ, Boyden ES, Murphy GJ, da Costa NM, Waters J, Li L, Tasic B, Zeng H. Cell. 2018 Jul 12;174(2):465-480.e22. doi: 10.1016/j.cell.2018.06.035. PubMed Associated Plasmids Ai139 (TIT2L-GFP-ICL-TPT) targeting vector Ai140 (TIT2L-GFP-ICL-tTA2) targeting vector Ai148 (TIT2L-GC6f-ICL-tTA2) targeting vector Ai161 (TIT2L-GFP-ICR-tTA2) targeting vector Ai163 (TIT2L-GC6s-ICL-TPT) targeting vector More ...
MRI Is a DNA Damage Response Adaptor during Classical Non-homologous End Joining. Hung PJ, Johnson B, Chen BR, Byrum AK, Bredemeyer AL, Yewdell WT, Johnson TE, Lee BJ, Deivasigamani S, Hindi I, Amatya P, Gross ML, Paull TT, Pisapia DJ, Chaudhuri J, Petrini JJH, Mosammaparast N, Amarasinghe GK, Zha S, Tyler JK, Sleckman BP. Mol Cell. 2018 Jul 19;71(2):332-342.e8. doi: 10.1016/j.molcel.2018.06.018. Epub 2018 Jul 12. PubMed
Bone marrow derived mesenchymal stem cells ameliorate inflammatory response in an in vitro model of familial hemophagocytic lymphohistiocytosis 2. Sevim H, Kocaefe YC, Onur MA, Uckan-Cetinkaya D, Gurpinar OA. Stem Cell Res Ther. 2018 Jul 18;9(1):198. doi: 10.1186/s13287-018-0941-y. PubMed
The shieldin complex mediates 53BP1-dependent DNA repair. Noordermeer SM, Adam S, Setiaputra D, Barazas M, Pettitt SJ, Ling AK, Olivieri M, Alvarez-Quilon A, Moatti N, Zimmermann M, Annunziato S, Krastev DB, Song F, Brandsma I, Frankum J, Brough R, Sherker A, Landry S, Szilard RK, Munro MM, McEwan A, Goullet de Rugy T, Lin ZY, Hart T, Moffat J, Gingras AC, Martin A, van Attikum H, Jonkers J, Lord CJ, Rottenberg S, Durocher D. Nature. 2018 Aug;560(7716):117-121. doi: 10.1038/s41586-018-0340-7. Epub 2018 Jul 18. PubMed Associated Plasmids pcDNA5-FRT/TO-FLAG-SHLD1 pCW-eGFP-SHLD1 pcDNA5-FRT/TO-FLAG-SHLD2 pGLUE-SHLD2 pCW-eGFP-SHLD2 More ...
HUWE1 controls the development of non-small cell lung cancer through down-regulation of p53. Yang D, Cheng D, Tu Q, Yang H, Sun B, Yan L, Dai H, Luo J, Mao B, Cao Y, Yu X, Jiang H, Zhao X. Theranostics. 2018 Jun 6;8(13):3517-3529. doi: 10.7150/thno.24401. eCollection 2018. PubMed
Translational Control through Differential Ribosome Pausing during Amino Acid Limitation in Mammalian Cells. Darnell AM, Subramaniam AR, O'Shea EK. Mol Cell. 2018 Jul 19;71(2):229-243.e11. doi: 10.1016/j.molcel.2018.06.041. PubMed
Exposure to galactic cosmic radiation compromises DNA repair and increases the potential for oncogenic chromosomal rearrangement in bronchial epithelial cells. Li Z, Jella KK, Jaafar L, Li S, Park S, Story MD, Wang H, Wang Y, Dynan WS. Sci Rep. 2018 Jul 23;8(1):11038. doi: 10.1038/s41598-018-29350-5. PubMed
Genetically engineered cerebral organoids model brain tumor formation. Bian S, Repic M, Guo Z, Kavirayani A, Burkard T, Bagley JA, Krauditsch C, Knoblich JA. Nat Methods. 2018 Aug;15(8):631-639. doi: 10.1038/s41592-018-0070-7. Epub 2018 Jul 23. PubMed
Engineering Point Mutant and Epitope-Tagged Alleles in Mice Using Cas9 RNA-Guided Nuclease. Gertsenstein M, Nutter LMJ. Curr Protoc Mouse Biol. 2018 Mar;8(1):28-53. doi: 10.1002/cpmo.40. PubMed
53BP1 cooperation with the REV7-shieldin complex underpins DNA structure-specific NHEJ. Ghezraoui H, Oliveira C, Becker JR, Bilham K, Moralli D, Anzilotti C, Fischer R, Deobagkar-Lele M, Sanchiz-Calvo M, Fueyo-Marcos E, Bonham S, Kessler BM, Rottenberg S, Cornall RJ, Green CM, Chapman JR. Nature. 2018 Aug;560(7716):122-127. doi: 10.1038/s41586-018-0362-1. Epub 2018 Jul 25. PubMed
Chemogenetic Isolation Reveals Synaptic Contribution of delta GABAA Receptors in Mouse Dentate Granule Neurons. Sun MY, Shu HJ, Benz A, Bracamontes J, Akk G, Zorumski CF, Steinbach JH, Mennerick SJ. J Neurosci. 2018 Sep 19;38(38):8128-8145. doi: 10.1523/JNEUROSCI.0799-18.2018. Epub 2018 Aug 3. PubMed
Somatic Depdc5 deletion recapitulates electroclinical features of human focal cortical dysplasia type IIA. Hu S, Knowlton RC, Watson BO, Glanowska KM, Murphy GG, Parent JM, Wang Y. Ann Neurol. 2018 Jul;84(1):140-146. doi: 10.1002/ana.25272. Epub 2018 Aug 6. PubMed
Reconstruction of the cell entry pathway of an extinct virus. Robinson-McCarthy LR, McCarthy KR, Raaben M, Piccinotti S, Nieuwenhuis J, Stubbs SH, Bakkers MJG, Whelan SPJ. PLoS Pathog. 2018 Aug 6;14(8):e1007123. doi: 10.1371/journal.ppat.1007123. eCollection 2018 Aug. PubMed
Disruption of C1galt1 Gene Promotes Development and Metastasis of Pancreatic Adenocarcinomas in Mice. Chugh S, Barkeer S, Rachagani S, Nimmakayala RK, Perumal N, Pothuraju R, Atri P, Mahapatra S, Thapa I, Talmon GA, Smith LM, Yu X, Neelamegham S, Fu J, Xia L, Ponnusamy MP, Batra SK. Gastroenterology. 2018 Nov;155(5):1608-1624. doi: 10.1053/j.gastro.2018.08.007. Epub 2018 Aug 4. PubMed
A dual fluorescence reporter system for high throughput screening of effectors of Kiss1 gene expression. Li X, Wang S, Lu Y, Yin H, Xiao J, Li K, Ma L, Zhou Y. FEBS Open Bio. 2018 Jul 3;8(8):1352-1363. doi: 10.1002/2211-5463.12476. eCollection 2018 Aug. PubMed
Live cell imaging of X chromosome reactivation during somatic cell reprogramming. Tran THY, Fukuda A, Aizawa S, Bui PL, Hayashi Y, Nishimura K, Hisatake K. Biochem Biophys Rep. 2018 Jul 24;15:86-92. doi: 10.1016/j.bbrep.2018.07.007. eCollection 2018 Sep. PubMed
Temporal and Spatial Epigenome Editing Allows Precise Gene Regulation in Mammalian Cells. Kuscu C, Mammadov R, Czikora A, Unlu H, Tufan T, Fischer NL, Arslan S, Bekiranov S, Kanemaki M, Adli M. J Mol Biol. 2019 Jan 4;431(1):111-121. doi: 10.1016/j.jmb.2018.08.001. Epub 2018 Aug 9. PubMed
Tbx6 Induces Nascent Mesoderm from Pluripotent Stem Cells and Temporally Controls Cardiac versus Somite Lineage Diversification. Sadahiro T, Isomi M, Muraoka N, Kojima H, Haginiwa S, Kurotsu S, Tamura F, Tani H, Tohyama S, Fujita J, Miyoshi H, Kawamura Y, Goshima N, Iwasaki YW, Murano K, Saito K, Oda M, Andersen P, Kwon C, Uosaki H, Nishizono H, Fukuda K, Ieda M. Cell Stem Cell. 2018 Sep 6;23(3):382-395.e5. doi: 10.1016/j.stem.2018.07.001. Epub 2018 Aug 9. PubMed
Endogenous HIF2A reporter systems for high-throughput functional screening. Zaini MN, Patel SA, Syafruddin SE, Rodrigues P, Vanharanta S. Sci Rep. 2018 Aug 13;8(1):12063. doi: 10.1038/s41598-018-30499-2. PubMed
MIWI2 targets RNAs transcribed from piRNA-dependent regions to drive DNA methylation in mouse prospermatogonia. Watanabe T, Cui X, Yuan Z, Qi H, Lin H. EMBO J. 2018 Sep 14;37(18). pii: embj.201695329. doi: 10.15252/embj.201695329. Epub 2018 Aug 14. PubMed
CRISPR/Cas9-Mediated In Situ Correction of LAMB3 Gene in Keratinocytes Derived from a Junctional Epidermolysis Bullosa Patient. Benati D, Miselli F, Cocchiarella F, Patrizi C, Carretero M, Baldassarri S, Ammendola V, Has C, Colloca S, Del Rio M, Larcher F, Recchia A. Mol Ther. 2018 Nov 7;26(11):2592-2603. doi: 10.1016/j.ymthe.2018.07.024. Epub 2018 Aug 4. PubMed
Loss of Kif18A Results in Spindle Assembly Checkpoint Activation at Microtubule-Attached Kinetochores. Janssen LME, Averink TV, Blomen VA, Brummelkamp TR, Medema RH, Raaijmakers JA. Curr Biol. 2018 Sep 10;28(17):2685-2696.e4. doi: 10.1016/j.cub.2018.06.026. Epub 2018 Aug 16. PubMed
Knock-In Mice with Myo3a Y137C Mutation Displayed Progressive Hearing Loss and Hair Cell Degeneration in the Inner Ear. Li P, Wen Z, Zhang G, Zhang A, Fu X, Gao J. Neural Plast. 2018 Jul 5;2018:4372913. doi: 10.1155/2018/4372913. eCollection 2018. PubMed
De Novo Mutations Activating Germline TP53 in an Inherited Bone-Marrow-Failure Syndrome. Toki T, Yoshida K, Wang R, Nakamura S, Maekawa T, Goi K, Katoh MC, Mizuno S, Sugiyama F, Kanezaki R, Uechi T, Nakajima Y, Sato Y, Okuno Y, Sato-Otsubo A, Shiozawa Y, Kataoka K, Shiraishi Y, Sanada M, Chiba K, Tanaka H, Terui K, Sato T, Kamio T, Sakaguchi H, Ohga S, Kuramitsu M, Hamaguchi I, Ohara A, Kanno H, Miyano S, Kojima S, Ishiguro A, Sugita K, Kenmochi N, Takahashi S, Eto K, Ogawa S, Ito E. Am J Hum Genet. 2018 Sep 6;103(3):440-447. doi: 10.1016/j.ajhg.2018.07.020. Epub 2018 Aug 23. PubMed
Nucleus of the Solitary Tract Serotonin 5-HT2C Receptors Modulate Food Intake. D'Agostino G, Lyons D, Cristiano C, Lettieri M, Olarte-Sanchez C, Burke LK, Greenwald-Yarnell M, Cansell C, Doslikova B, Georgescu T, Martinez de Morentin PB, Myers MG Jr, Rochford JJ, Heisler LK. Cell Metab. 2018 Oct 2;28(4):619-630.e5. doi: 10.1016/j.cmet.2018.07.017. Epub 2018 Aug 23. PubMed
Evaluation of Organic Anion Transporter 1A2-knock-in Mice as a Model of Human Blood-brain Barrier. Sano Y, Mizuno T, Mochizuki T, Uchida Y, Umetsu M, Terasaki T, Kusuhara H. Drug Metab Dispos. 2018 Nov;46(11):1767-1775. doi: 10.1124/dmd.118.081877. Epub 2018 Aug 28. PubMed
Muscarinic Acetylcholine Receptors Chrm1 and Chrm3 Are Essential for REM Sleep. Niwa Y, Kanda GN, Yamada RG, Shi S, Sunagawa GA, Ukai-Tadenuma M, Fujishima H, Matsumoto N, Masumoto KH, Nagano M, Kasukawa T, Galloway J, Perrin D, Shigeyoshi Y, Ukai H, Kiyonari H, Sumiyama K, Ueda HR. Cell Rep. 2018 Aug 28;24(9):2231-2247.e7. doi: 10.1016/j.celrep.2018.07.082. PubMed
Tprn is essential for the integrity of stereociliary rootlet in cochlear hair cells in mice. Men Y, Li X, Tu H, Zhang A, Fu X, Wang Z, Jin Y, Hou C, Zhang T, Zhang S, Zhou Y, Li B, Li J, Sun X, Wang H, Gao J. Front Med. 2018 Aug 30. pii: 10.1007/s11684-018-0638-8. doi: 10.1007/s11684-018-0638-8. PubMed
Interferon priming is essential for human CD34+ cell-derived plasmacytoid dendritic cell maturation and function. Laustsen A, Bak RO, Krapp C, Kjaer L, Egedahl JH, Petersen CC, Pillai S, Tang HQ, Uldbjerg N, Porteus M, Roan NR, Nyegaard M, Denton PW, Jakobsen MR. Nat Commun. 2018 Aug 30;9(1):3525. doi: 10.1038/s41467-018-05816-y. PubMed
Rapid Disruption of Genes Specifically in Livers of Mice Using Multiplex CRISPR/Cas9 Editing. Pankowicz FP, Barzi M, Kim KH, Legras X, Martins CS, Wooton-Kee CR, Lagor WR, Marini JC, Elsea SH, Bissig-Choisat B, Moore DD, Bissig KD. Gastroenterology. 2018 Dec;155(6):1967-1970.e6. doi: 10.1053/j.gastro.2018.08.037. Epub 2018 Aug 28. PubMed
RSPH6A is required for sperm flagellum formation and male fertility in mice. Abbasi F, Miyata H, Shimada K, Morohoshi A, Nozawa K, Matsumura T, Xu Z, Pratiwi P, Ikawa M. J Cell Sci. 2018 Oct 11;131(19). pii: jcs.221648. doi: 10.1242/jcs.221648. PubMed
4D cell biology: big data image analytics and lattice light-sheet imaging reveal dynamics of clathrin-mediated endocytosis in stem cell-derived intestinal organoids. Schoneberg J, Dambournet D, Liu TL, Forster R, Hockemeyer D, Betzig E, Drubin DG. Mol Biol Cell. 2018 Nov 26;29(24):2959-2968. doi: 10.1091/mbc.E18-06-0375. Epub 2018 Sep 6. PubMed
Whole exome sequencing of ENU-induced thrombosis modifier mutations in the mouse. Tomberg K, Westrick RJ, Kotnik EN, Cleuren AC, Siemieniak DR, Zhu G, Saunders TL, Ginsburg D. PLoS Genet. 2018 Sep 6;14(9):e1007658. doi: 10.1371/journal.pgen.1007658. eCollection 2018 Sep. PubMed
One-Vector System for Multiplexed CRISPR/Cas9 against Hepatitis B Virus cccDNA Utilizing High-Capacity Adenoviral Vectors. Schiwon M, Ehrke-Schulz E, Oswald A, Bergmann T, Michler T, Protzer U, Ehrhardt A. Mol Ther Nucleic Acids. 2018 Sep 7;12:242-253. doi: 10.1016/j.omtn.2018.05.006. Epub 2018 Jun 7. PubMed
VSV-G-Enveloped Vesicles for Traceless Delivery of CRISPR-Cas9. Montagna C, Petris G, Casini A, Maule G, Franceschini GM, Zanella I, Conti L, Arnoldi F, Burrone OR, Zentilin L, Zacchigna S, Giacca M, Cereseto A. Mol Ther Nucleic Acids. 2018 Sep 7;12:453-462. doi: 10.1016/j.omtn.2018.05.010. Epub 2018 Jul 11. PubMed
3-Aminobenzamide Prevents Concanavalin A-Induced Acute Hepatitis by an Anti-inflammatory and Anti-oxidative Mechanism. Wardi J, Ernst O, Lilja A, Aeed H, Katz S, Ben-Nachum I, Ben-Dror I, Katz D, Bernadsky O, Kandhikonda R, Avni Y, Fraser IDC, Weinstain R, Biro A, Zor T. Dig Dis Sci. 2018 Dec;63(12):3382-3397. doi: 10.1007/s10620-018-5267-1. Epub 2018 Sep 8. PubMed
Co-expression of sperm membrane proteins CMTM2A and CMTM2B is essential for ADAM3 localization and male fertility in mice. Fujihara Y, Oji A, Kojima-Kita K, Larasati T, Ikawa M. J Cell Sci. 2018 Oct 8;131(19). pii: jcs.221481. doi: 10.1242/jcs.221481. PubMed
Opposing Roles of FANCJ and HLTF Protect Forks and Restrain Replication during Stress. Peng M, Cong K, Panzarino NJ, Nayak S, Calvo J, Deng B, Zhu LJ, Morocz M, Hegedus L, Haracska L, Cantor SB. Cell Rep. 2018 Sep 18;24(12):3251-3261. doi: 10.1016/j.celrep.2018.08.065. PubMed
Systematic genetic mapping of necroptosis identifies SLC39A7 as modulator of death receptor trafficking. Fauster A, Rebsamen M, Willmann KL, Cesar-Razquin A, Girardi E, Bigenzahn JW, Schischlik F, Scorzoni S, Bruckner M, Konecka J, Hormann K, Heinz LX, Boztug K, Superti-Furga G. Cell Death Differ. 2018 Sep 20. pii: 10.1038/s41418-018-0192-6. doi: 10.1038/s41418-018-0192-6. PubMed
PAXX Participates in Base Excision Repair via Interacting with Pol beta and Contributes to TMZ Resistance in Glioma Cells. Yang B, Fu X, Hao J, Sun J, Li Z, Li H, Xu H. J Mol Neurosci. 2018 Oct;66(2):214-221. doi: 10.1007/s12031-018-1157-4. Epub 2018 Sep 20. PubMed
Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants. Toth E, Czene BC, Kulcsar PI, Krausz SL, Talas A, Nyeste A, Varga E, Huszar K, Weinhardt N, Ligeti Z, Borsy AE, Fodor E, Welker E. Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815. PubMed Associated Plasmids pTE4495 pTE4497 WN10150 pTE4565 pTE4566 More ...
Myostatin knockout induces apoptosis in human cervical cancer cells via elevated reactive oxygen species generation. Han YQ, Ming SL, Wu HT, Zeng L, Ba G, Li J, Lu WF, Han J, Du QJ, Sun MM, Yang GY, Wang J, Chu BB. Redox Biol. 2018 Oct;19:412-428. doi: 10.1016/j.redox.2018.09.009. Epub 2018 Sep 13. PubMed
NPC1L1-dependent intestinal cholesterol absorption requires ganglioside GM3 in membrane microdomains. Nihei W, Nagafuku M, Hayamizu H, Odagiri Y, Tamura Y, Kikuchi Y, Veillon L, Kanoh H, Inamori KI, Arai K, Kabayama K, Fukase K, Inokuchi JI. J Lipid Res. 2018 Nov;59(11):2181-2187. doi: 10.1194/jlr.M089201. Epub 2018 Sep 21. PubMed
ETV7 is an essential component of a rapamycin-insensitive mTOR complex in cancer. Harwood FC, Klein Geltink RI, O'Hara BP, Cardone M, Janke L, Finkelstein D, Entin I, Paul L, Houghton PJ, Grosveld GC. Sci Adv. 2018 Sep 12;4(9):eaar3938. doi: 10.1126/sciadv.aar3938. eCollection 2018 Sep. PubMed
Infection with flaviviruses requires BCLXL for cell survival. Suzuki T, Okamoto T, Katoh H, Sugiyama Y, Kusakabe S, Tokunaga M, Hirano J, Miyata Y, Fukuhara T, Ikawa M, Satoh T, Yoshio S, Suzuki R, Saijo M, Huang DCS, Kanto T, Akira S, Matsuura Y. PLoS Pathog. 2018 Sep 27;14(9):e1007299. doi: 10.1371/journal.ppat.1007299. eCollection 2018 Sep. PubMed
AP-4 vesicles contribute to spatial control of autophagy via RUSC-dependent peripheral delivery of ATG9A. Davies AK, Itzhak DN, Edgar JR, Archuleta TL, Hirst J, Jackson LP, Robinson MS, Borner GHH. Nat Commun. 2018 Sep 27;9(1):3958. doi: 10.1038/s41467-018-06172-7. PubMed
Simultaneous zygotic inactivation of multiple genes in mouse through CRISPR/Cas9-mediated base editing. Zhang H, Pan H, Zhou C, Wei Y, Ying W, Li S, Wang G, Li C, Ren Y, Li G, Ding X, Sun Y, Li GL, Song L, Li Y, Yang H, Liu Z. Development. 2018 Oct 17;145(20). pii: dev.168906. doi: 10.1242/dev.168906. PubMed
miR-191 modulates B-cell development and targets transcription factors E2A, Foxp1, and Egr1. Blume J, Zietara N, Witzlau K, Liu Y, Sanchez OO, Puchalka J, Winter SJ, Kunze-Schumacher H, Saran N, Duber S, Roy B, Weiss S, Klein C, Wurst W, Lyszkiewicz M, Krueger A. Eur J Immunol. 2019 Jan;49(1):121-132. doi: 10.1002/eji.201847660. Epub 2018 Oct 22. PubMed
A sheep model of cystic fibrosis generated by CRISPR/Cas9 disruption of the CFTR gene. Fan Z, Perisse IV, Cotton CU, Regouski M, Meng Q, Domb C, Van Wettere AJ, Wang Z, Harris A, White KL, Polejaeva IA. JCI Insight. 2018 Oct 4;3(19). pii: 123529. doi: 10.1172/jci.insight.123529. PubMed
Complement Component C3 Is Highly Expressed in Human Pancreatic Islets and Prevents beta Cell Death via ATG16L1 Interaction and Autophagy Regulation. King BC, Kulak K, Krus U, Rosberg R, Golec E, Wozniak K, Gomez MF, Zhang E, O'Connell DJ, Renstrom E, Blom AM. Cell Metab. 2019 Jan 8;29(1):202-210.e6. doi: 10.1016/j.cmet.2018.09.009. Epub 2018 Oct 4. PubMed
In utero CRISPR-mediated therapeutic editing of metabolic genes. Rossidis AC, Stratigis JD, Chadwick AC, Hartman HA, Ahn NJ, Li H, Singh K, Coons BE, Li L, Lv W, Zoltick PW, Alapati D, Zacharias W, Jain R, Morrisey EE, Musunuru K, Peranteau WH. Nat Med. 2018 Oct;24(10):1513-1518. doi: 10.1038/s41591-018-0184-6. Epub 2018 Oct 8. PubMed
Paired CRISPR/Cas9 Nickases Mediate Efficient Site-Specific Integration of F9 into rDNA Locus of Mouse ESCs. Wang Y, Zhao J, Duan N, Liu W, Zhang Y, Zhou M, Hu Z, Feng M, Liu X, Wu L, Li Z, Liang D. Int J Mol Sci. 2018 Oct 5;19(10). pii: ijms19103035. doi: 10.3390/ijms19103035. PubMed
Mechanisms of integrin alphaVbeta5 clustering in flat clathrin lattices. Zuidema A, Wang W, Kreft M, Te Molder L, Hoekman L, Bleijerveld OB, Nahidiazar L, Janssen H, Sonnenberg A. J Cell Sci. 2018 Nov 5;131(21). pii: jcs.221317. doi: 10.1242/jcs.221317. PubMed
EIF1AX and RAS Mutations Cooperate to Drive Thyroid Tumorigenesis through ATF4 and c-MYC. Krishnamoorthy GP, Davidson NR, Leach SD, Zhao Z, Lowe SW, Lee G, Landa I, Nagarajah J, Saqcena M, Singh K, Wendel HG, Dogan S, Tamarapu PP, Blenis J, Ghossein RA, Knauf JA, Ratsch G, Fagin JA. Cancer Discov. 2019 Feb;9(2):264-281. doi: 10.1158/2159-8290.CD-18-0606. Epub 2018 Oct 10. PubMed
Apolipoprotein E deficiency accelerates atherosclerosis development in miniature pigs. Fang B, Ren X, Wang Y, Li Z, Zhao L, Zhang M, Li C, Zhang Z, Chen L, Li X, Liu J, Xiong Q, Zhang L, Jin Y, Liu X, Li L, Wei H, Yang H, Li R, Dai Y. Dis Model Mech. 2018 Oct 10;11(10). pii: 11/10/dmm036632. doi: 10.1242/dmm.036632. PubMed
Neural blastocyst complementation enables mouse forebrain organogenesis. Chang AN, Liang Z, Dai HQ, Chapdelaine-Williams AM, Andrews N, Bronson RT, Schwer B, Alt FW. Nature. 2018 Nov;563(7729):126-130. doi: 10.1038/s41586-018-0586-0. Epub 2018 Oct 10. PubMed
CDCA7 and HELLS mutations undermine nonhomologous end joining in centromeric instability syndrome. Unoki M, Funabiki H, Velasco G, Francastel C, Sasaki H. J Clin Invest. 2019 Jan 2;129(1):78-92. doi: 10.1172/JCI99751. Epub 2018 Nov 19. PubMed
Induction of selective autophagy in cells replicating hepatitis C virus genome. Mori H, Fukuhara T, Ono C, Tamura T, Sato A, Fauzyah Y, Wada M, Okamoto T, Noda T, Yoshimori T, Matsuura Y. J Gen Virol. 2018 Dec;99(12):1643-1657. doi: 10.1099/jgv.0.001161. Epub 2018 Oct 12. PubMed
HIT-Cas9: A CRISPR/Cas9 Genome-Editing Device under Tight and Effective Drug Control. Zhao C, Zhao Y, Zhang J, Lu J, Chen L, Zhang Y, Ying Y, Xu J, Wei S, Wang Y. Mol Ther Nucleic Acids. 2018 Dec 7;13:208-219. doi: 10.1016/j.omtn.2018.08.022. Epub 2018 Sep 1. PubMed Associated Plasmids EF1a-Cas9-2XNES-2ERT2
Generating Loss-of-function iPSC Lines with Combined CRISPR Indel Formation and Reprogramming from Human Fibroblasts. Tidball AM, Swaminathan P, Dang LT, Parent JM. Bio Protoc. 2018 Apr 5;8(7). doi: 10.21769/BioProtoc.2794. PubMed
Harnessing accurate non-homologous end joining for efficient precise deletion in CRISPR/Cas9-mediated genome editing. Guo T, Feng YL, Xiao JJ, Liu Q, Sun XN, Xiang JF, Kong N, Liu SC, Chen GQ, Wang Y, Dong MM, Cai Z, Lin H, Cai XJ, Xie AY. Genome Biol. 2018 Oct 19;19(1):170. doi: 10.1186/s13059-018-1518-x. PubMed
Targeted integration in human cells through single crossover mediated by ZFN or CRISPR/Cas9. Liu X, Wang M, Qin Y, Shi X, Cong P, Chen Y, He Z. BMC Biotechnol. 2018 Oct 19;18(1):66. doi: 10.1186/s12896-018-0474-6. PubMed
TRIP13 and APC15 drive mitotic exit by turnover of interphase- and unattached kinetochore-produced MCC. Kim DH, Han JS, Ly P, Ye Q, McMahon MA, Myung K, Corbett KD, Cleveland DW. Nat Commun. 2018 Oct 19;9(1):4354. doi: 10.1038/s41467-018-06774-1. PubMed
Astrocyte-Secreted Chordin-like 1 Drives Synapse Maturation and Limits Plasticity by Increasing Synaptic GluA2 AMPA Receptors. Blanco-Suarez E, Liu TF, Kopelevich A, Allen NJ. Neuron. 2018 Dec 5;100(5):1116-1132.e13. doi: 10.1016/j.neuron.2018.09.043. Epub 2018 Oct 18. PubMed
A Feedforward Mechanism Mediated by Mechanosensitive Ion Channel PIEZO1 and Tissue Mechanics Promotes Glioma Aggression. Chen X, Wanggou S, Bodalia A, Zhu M, Dong W, Fan JJ, Yin WC, Min HK, Hu M, Draghici D, Dou W, Li F, Coutinho FJ, Whetstone H, Kushida MM, Dirks PB, Song Y, Hui CC, Sun Y, Wang LY, Li X, Huang X. Neuron. 2018 Nov 21;100(4):799-815.e7. doi: 10.1016/j.neuron.2018.09.046. Epub 2018 Oct 18. PubMed
RIG-I is responsible for activation of type I interferon pathway in Seneca Valley virus-infected porcine cells to suppress viral replication. Li P, Zhang X, Cao W, Yang F, Du X, Shi Z, Zhang M, Liu X, Zhu Z, Zheng H. Virol J. 2018 Oct 23;15(1):162. doi: 10.1186/s12985-018-1080-x. PubMed
Analysis of the relationship between the KRAS G12V oncogene and the Hippo effector YAP1 in embryonal rhabdomyosarcoma. Mohamed AD, Shah N, Hettmer S, Vargesson N, Wackerhage H. Sci Rep. 2018 Oct 23;8(1):15674. doi: 10.1038/s41598-018-33852-7. PubMed
Generating Genetically Engineered Mice Using a Spermatogonial Stem Cell-Mediated Method. Sato T, Ogawa T. Methods Mol Biol. 2019;1874:87-98. doi: 10.1007/978-1-4939-8831-0_5. PubMed
Gene Knockouts in Goats Using CRISPR/Cas9 System and Somatic Cell Nuclear Transfer. Fan Z, Yang M, Regouski M, Polejaeva IA. Methods Mol Biol. 2019;1874:373-390. doi: 10.1007/978-1-4939-8831-0_22. PubMed
Mice harboring an MCTO mutation exhibit renal failure resembling nephropathy in human patients. Tsunakawa Y, Hamada M, Matsunaga Y, Fuseya S, Jeon H, Wakimoto Y, Usui T, Kanai M, Mizuno S, Morito N, Takahashi S. Exp Anim. 2019 Feb 26;68(1):103-111. doi: 10.1538/expanim.18-0093. Epub 2018 Oct 26. PubMed
Efficient genome editing using CRISPR-Cas-mediated homology directed repair in the ascidian Ciona robusta. Pickett CJ, Zeller RW. Genesis. 2018 Dec;56(11-12):e23260. doi: 10.1002/dvg.23260. Epub 2018 Nov 30. PubMed
Shared and distinct transcriptomic cell types across neocortical areas. Tasic B, Yao Z, Graybuck LT, Smith KA, Nguyen TN, Bertagnolli D, Goldy J, Garren E, Economo MN, Viswanathan S, Penn O, Bakken T, Menon V, Miller J, Fong O, Hirokawa KE, Lathia K, Rimorin C, Tieu M, Larsen R, Casper T, Barkan E, Kroll M, Parry S, Shapovalova NV, Hirschstein D, Pendergraft J, Sullivan HA, Kim TK, Szafer A, Dee N, Groblewski P, Wickersham I, Cetin A, Harris JA, Levi BP, Sunkin SM, Madisen L, Daigle TL, Looger L, Bernard A, Phillips J, Lein E, Hawrylycz M, Svoboda K, Jones AR, Koch C, Zeng H. Nature. 2018 Nov;563(7729):72-78. doi: 10.1038/s41586-018-0654-5. Epub 2018 Oct 31. PubMed
Characterization of a Mouse Model of Borjeson-Forssman-Lehmann Syndrome. Cheng C, Deng PY, Ikeuchi Y, Yuede C, Li D, Rensing N, Huang J, Baldridge D, Maloney SE, Dougherty JD, Constantino J, Jahani-Asl A, Wong M, Wozniak DF, Wang T, Klyachko VA, Bonni A. Cell Rep. 2018 Nov 6;25(6):1404-1414.e6. doi: 10.1016/j.celrep.2018.10.043. PubMed
The nucleosome remodeling and deacetylase complex protein CHD4 regulates neural differentiation of mouse embryonic stem cells by down-regulating p53. Hirota A, Nakajima-Koyama M, Ashida Y, Nishida E. J Biol Chem. 2019 Jan 4;294(1):195-209. doi: 10.1074/jbc.RA118.004086. Epub 2018 Nov 8. PubMed
Exosomes exert cardioprotection in dystrophin-deficient cardiomyocytes via ERK1/2-p38/MAPK signaling. Gartz M, Darlington A, Afzal MZ, Strande JL. Sci Rep. 2018 Nov 8;8(1):16519. doi: 10.1038/s41598-018-34879-6. PubMed
Translesion polymerase kappa-dependent DNA synthesis underlies replication fork recovery. Tonzi P, Yin Y, Lee CWT, Rothenberg E, Huang TT. Elife. 2018 Nov 13;7. pii: 41426. doi: 10.7554/eLife.41426. PubMed
N-terminal acetylation and the N-end rule pathway control degradation of the lipid droplet protein PLIN2. Nguyen KT, Lee CS, Mun SH, Truong NT, Park SK, Hwang CS. J Biol Chem. 2019 Jan 4;294(1):379-388. doi: 10.1074/jbc.RA118.005556. Epub 2018 Nov 13. PubMed
Cellular Zinc Finger Protein 622 Hinders Human Adenovirus Lytic Growth and Limits Binding of the Viral pVII Protein to Virus DNA. Mun K, Punga T. J Virol. 2019 Jan 17;93(3). pii: JVI.01628-18. doi: 10.1128/JVI.01628-18. Print 2019 Feb 1. PubMed
Linking a cell-division gene and a suicide gene to define and improve cell therapy safety. Liang Q, Monetti C, Shutova MV, Neely EJ, Hacibekiroglu S, Yang H, Kim C, Zhang P, Li C, Nagy K, Mileikovsky M, Gyongy I, Sung HK, Nagy A. Nature. 2018 Nov;563(7733):701-704. doi: 10.1038/s41586-018-0733-7. Epub 2018 Nov 14. PubMed
Core fucose is essential glycosylation for CD14-dependent Toll-like receptor 4 and Toll-like receptor 2 signalling in macrophages. Nakayama K, Wakamatsu K, Fujii H, Shinzaki S, Takamatsu S, Kitazume S, Kamada Y, Takehara T, Taniguchi N, Miyoshi E. J Biochem. 2019 Mar 1;165(3):227-237. doi: 10.1093/jb/mvy098. PubMed
Splicing of long non-coding RNAs primarily depends on polypyrimidine tract and 5' splice-site sequences due to weak interactions with SR proteins. Krchnakova Z, Thakur PK, Krausova M, Bieberstein N, Haberman N, Muller-McNicoll M, Stanek D. Nucleic Acids Res. 2019 Jan 25;47(2):911-928. doi: 10.1093/nar/gky1147. PubMed
A hPSC-based platform to discover gene-environment interactions that impact human beta-cell and dopamine neuron survival. Zhou T, Kim TW, Chong CN, Tan L, Amin S, Sadat Badieyan Z, Mukherjee S, Ghazizadeh Z, Zeng H, Guo M, Crespo M, Zhang T, Kenyon R, Robinson CL, Apostolou E, Wang H, Xiang JZ, Evans T, Studer L, Chen S. Nat Commun. 2018 Nov 16;9(1):4815. doi: 10.1038/s41467-018-07201-1. PubMed
A B-Cell-Specific Enhancer Orchestrates Nuclear Architecture to Generate a Diverse Antigen Receptor Repertoire. Barajas-Mora EM, Kleiman E, Xu J, Carrico NC, Lu H, Oltz EM, Murre C, Feeney AJ. Mol Cell. 2019 Jan 3;73(1):48-60.e5. doi: 10.1016/j.molcel.2018.10.013. Epub 2018 Nov 15. PubMed
A novel cancer syndrome caused by KCNQ1-deficiency in the golden Syrian hamster. Li R, Miao J, Tabaran AF, O'Sullivan MG, Anderson KJ, Scott PM, Wang Z, Cormier RT. J Carcinog. 2018 Oct 10;17:6. doi: 10.4103/jcar.JCar_5_18. eCollection 2018. PubMed
Lack of LTbetaR Increases Susceptibility of IPEC-J2 Cells to Porcine Epidemic Diarrhea Virus. Altawaty T, Liu L, Zhang H, Tao C, Hou S, Li K, Wang Y. Cells. 2018 Nov 21;7(11). pii: cells7110222. doi: 10.3390/cells7110222. PubMed
Esrp1-Regulated Splicing of Arhgef11 Isoforms Is Required for Epithelial Tight Junction Integrity. Lee S, Cieply B, Yang Y, Peart N, Glaser C, Chan P, Carstens RP. Cell Rep. 2018 Nov 27;25(9):2417-2430.e5. doi: 10.1016/j.celrep.2018.10.097. PubMed
CDK12 regulates DNA repair genes by suppressing intronic polyadenylation. Dubbury SJ, Boutz PL, Sharp PA. Nature. 2018 Dec;564(7734):141-145. doi: 10.1038/s41586-018-0758-y. Epub 2018 Nov 28. PubMed Associated Plasmids Cdk12_intron3_sg2_pX330 Cdk12_intron4_sg1_pX458 pCR8/GW_TOPO_Cdk12_Untagged pCR8/GW_TOPO_Cdk12_Nterm_FlagHa PB-Neo-TetO-Cdk12_Untagged More ...
Disruption of the ZBED6 binding site in intron 3 of IGF2 by CRISPR/Cas9 leads to enhanced muscle development in Liang Guang Small Spotted pigs. Liu X, Liu H, Wang M, Li R, Zeng J, Mo D, Cong P, Liu X, Chen Y, He Z. Transgenic Res. 2019 Feb;28(1):141-150. doi: 10.1007/s11248-018-0107-9. Epub 2018 Nov 28. PubMed
A positive feedback mechanism ensures proper assembly of the functional inner centromere during mitosis in human cells. Liang C, Zhang Z, Chen Q, Yan H, Zhang M, Xiang X, Yi Q, Pan X, Cheng H, Wang F. J Biol Chem. 2019 Feb 1;294(5):1437-1450. doi: 10.1074/jbc.RA118.006046. Epub 2018 Nov 29. PubMed
Efficient scarless genome editing in human pluripotent stem cells. Ikeda K, Uchida N, Nishimura T, White J, Martin RM, Nakauchi H, Sebastiano V, Weinberg KI, Porteus MH. Nat Methods. 2018 Dec;15(12):1045-1047. doi: 10.1038/s41592-018-0212-y. Epub 2018 Nov 30. PubMed Associated Plasmids pSLHDR01_mCherry_tCD19 pSLHDR02_EGFP_tCD8 pSLHDR03_mCherry_tCD19_unique guide pSLHDR_R1 pSLHDR_R4
CRISPR-Cas9-based Genome Engineering to Generate Jurkat Reporter Models for HIV-1 Infection with Selected Proviral Integration Sites. Bialek JK, Walther T, Hauber J, Lange UC. J Vis Exp. 2018 Nov 14;(141). doi: 10.3791/58572. PubMed
Low and variable tumor reactivity of the intratumoral TCR repertoire in human cancers. Scheper W, Kelderman S, Fanchi LF, Linnemann C, Bendle G, de Rooij MAJ, Hirt C, Mezzadra R, Slagter M, Dijkstra K, Kluin RJC, Snaebjornsson P, Milne K, Nelson BH, Zijlmans H, Kenter G, Voest EE, Haanen JBAG, Schumacher TN. Nat Med. 2019 Jan;25(1):89-94. doi: 10.1038/s41591-018-0266-5. Epub 2018 Dec 3. PubMed
The HDAC-Associated Sin3B Protein Represses DREAM Complex Targets and Cooperates with APC/C to Promote Quiescence. Bainor AJ, Saini S, Calderon A, Casado-Polanco R, Giner-Ramirez B, Moncada C, Cantor DJ, Ernlund A, Litovchick L, David G. Cell Rep. 2018 Dec 4;25(10):2797-2807.e8. doi: 10.1016/j.celrep.2018.11.024. PubMed
ULK1 O-GlcNAcylation Is Crucial for Activating VPS34 via ATG14L during Autophagy Initiation. Pyo KE, Kim CR, Lee M, Kim JS, Kim KI, Baek SH. Cell Rep. 2018 Dec 4;25(10):2878-2890.e4. doi: 10.1016/j.celrep.2018.11.042. PubMed
EZH2 variants differentially regulate polycomb repressive complex 2 in histone methylation and cell differentiation. Mu W, Starmer J, Yee D, Magnuson T. Epigenetics Chromatin. 2018 Dec 6;11(1):71. doi: 10.1186/s13072-018-0242-9. PubMed
Human nuclear RNAi-defective 2 (NRDE2) is an essential RNA splicing factor. Jiao AL, Perales R, Umbreit NT, Haswell JR, Piper ME, Adams BD, Pellman D, Kennedy S, Slack FJ. RNA. 2019 Mar;25(3):352-363. doi: 10.1261/rna.069773.118. Epub 2018 Dec 11. PubMed
Genetically modified pigs are protected from classical swine fever virus. Xie Z, Pang D, Yuan H, Jiao H, Lu C, Wang K, Yang Q, Li M, Chen X, Yu T, Chen X, Dai Z, Peng Y, Tang X, Li Z, Wang T, Guo H, Li L, Tu C, Lai L, Ouyang H. PLoS Pathog. 2018 Dec 13;14(12):e1007193. doi: 10.1371/journal.ppat.1007193. eCollection 2018 Dec. PubMed
APOPT1/COA8 assists COX assembly and is oppositely regulated by UPS and ROS. Signes A, Cerutti R, Dickson AS, Beninca C, Hinchy EC, Ghezzi D, Carrozzo R, Bertini E, Murphy MP, Nathan JA, Viscomi C, Fernandez-Vizarra E, Zeviani M. EMBO Mol Med. 2019 Jan;11(1). pii: emmm.201809582. doi: 10.15252/emmm.201809582. PubMed
Human sex reversal is caused by duplication or deletion of core enhancers upstream of SOX9. Croft B, Ohnesorg T, Hewitt J, Bowles J, Quinn A, Tan J, Corbin V, Pelosi E, van den Bergen J, Sreenivasan R, Knarston I, Robevska G, Vu DC, Hutson J, Harley V, Ayers K, Koopman P, Sinclair A. Nat Commun. 2018 Dec 14;9(1):5319. doi: 10.1038/s41467-018-07784-9. PubMed
Intrinsic Dynamics of a Human Gene Reveal the Basis of Expression Heterogeneity. Rodriguez J, Ren G, Day CR, Zhao K, Chow CC, Larson DR. Cell. 2019 Jan 10;176(1-2):213-226.e18. doi: 10.1016/j.cell.2018.11.026. Epub 2018 Dec 13. PubMed
Familial episodic limb pain in kindreds with novel Nav1.9 mutations. Kabata R, Okuda H, Noguchi A, Kondo D, Fujiwara M, Hata K, Kato Y, Ishikawa K, Tanaka M, Sekine Y, Hishikawa N, Mizukami T, Ito J, Akasaka M, Sakurai K, Yoshida T, Minoura H, Hayashi T, Inoshita K, Matsuyama M, Kinjo N, Cao Y, Inoue S, Kobayashi H, Harada KH, Youssefian S, Takahashi T, Koizumi A. PLoS One. 2018 Dec 17;13(12):e0208516. doi: 10.1371/journal.pone.0208516. eCollection 2018. PubMed
Hippo Pathway Regulation by Tyrosine Kinases. Reuven N, Shanzer M, Shaul Y. Methods Mol Biol. 2019;1893:215-236. doi: 10.1007/978-1-4939-8910-2_17. PubMed
Semaphorin 7A promotes EGFR-TKI resistance in EGFR mutant lung adenocarcinoma cells. Kinehara Y, Nagatomo I, Koyama S, Ito D, Nojima S, Kurebayashi R, Nakanishi Y, Suga Y, Nishijima-Futami Y, Osa A, Nakatani T, Kato Y, Nishide M, Hayama Y, Higashiguchi M, Morimura O, Miyake K, Kang S, Minami T, Hirata H, Iwahori K, Takimoto T, Takamatsu H, Takeda Y, Hosen N, Hoshino S, Shintani Y, Okumura M, Kumagai T, Nishino K, Imamura F, Nakatsuka SI, Kijima T, Kida H, Kumanogoh A. JCI Insight. 2018 Dec 20;3(24). pii: 123093. doi: 10.1172/jci.insight.123093. PubMed
Regulation of MLL/COMPASS stability through its proteolytic cleavage by taspase1 as a possible approach for clinical therapy of leukemia. Zhao Z, Wang L, Volk AG, Birch NW, Stoltz KL, Bartom ET, Marshall SA, Rendleman EJ, Nestler CM, Shilati J, Schiltz GE, Crispino JD, Shilatifard A. Genes Dev. 2019 Jan 1;33(1-2):61-74. doi: 10.1101/gad.319830.118. Epub 2018 Dec 20. PubMed
HLA-DO Modulates the Diversity of the MHC-II Self-peptidome. Nanaware PP, Jurewicz MM, Leszyk JD, Shaffer SA, Stern LJ. Mol Cell Proteomics. 2019 Mar;18(3):490-503. doi: 10.1074/mcp.RA118.000956. Epub 2018 Dec 20. PubMed
Tideglusib Rescues Neurite Pathology of SPG11 iPSC Derived Cortical Neurons. Pozner T, Schray A, Regensburger M, Lie DC, Schlotzer-Schrehardt U, Winkler J, Turan S, Winner B. Front Neurosci. 2018 Dec 6;12:914. doi: 10.3389/fnins.2018.00914. eCollection 2018. PubMed
Platforms of in vivo genome editing with inducible Cas9 for advanced cancer modeling. Jo N, Sogabe Y, Yamada Y, Ukai T, Kagawa H, Mitsunaga K, Woltjen K, Yamada Y. Cancer Sci. 2019 Mar;110(3):926-938. doi: 10.1111/cas.13924. Epub 2019 Jan 16. PubMed
Lactate dehydrogenase C is required for the protein expression of a sperm-specific isoform of lactate dehydrogenase A. Dodo M, Kitamura H, Shima H, Saigusa D, Wati SM, Ota N, Katsuoka F, Chiba H, Okae H, Arima T, Igarashi K, Koseki T, Sekine H, Motohashi H. J Biochem. 2019 Apr 1;165(4):323-334. doi: 10.1093/jb/mvy108. PubMed
Activation of Estrogen Receptor Alpha by Decitabine Inhibits Osteosarcoma Growth and Metastasis. Lillo Osuna MA, Garcia-Lopez J, El Ayachi I, Fatima I, Khalid AB, Kumpati J, Slayden AV, Seagroves TN, Miranda-Carboni GA, Krum SA. Cancer Res. 2019 Mar 15;79(6):1054-1068. doi: 10.1158/0008-5472.CAN-18-1255. Epub 2018 Dec 28. PubMed
An upstream enhancer regulates Gpihbp1 expression in a tissue-specific manner. Allan CM, Heizer PJ, Tu Y, Sandoval NP, Jung RS, Morales JE, Sajti E, Troutman TD, Saunders TL, Cusanovich DA, Beigneux AP, Romanoski CE, Fong LG, Young SG. J Lipid Res. 2019 Apr;60(4):869-879. doi: 10.1194/jlr.M091322. Epub 2018 Dec 31. PubMed
Curcumin relieved cisplatin-induced kidney inflammation through inhibiting Mincle-maintained M1 macrophage phenotype. Tan RZ, Liu J, Zhang YY, Wang HL, Li JC, Liu YH, Zhong X, Zhang YW, Yan Y, Lan HY, Wang L. Phytomedicine. 2019 Jan;52:284-294. doi: 10.1016/j.phymed.2018.09.210. Epub 2018 Sep 25. PubMed
DHHC4 and DHHC5 Facilitate Fatty Acid Uptake by Palmitoylating and Targeting CD36 to the Plasma Membrane. Wang J, Hao JW, Wang X, Guo H, Sun HH, Lai XY, Liu LY, Zhu M, Wang HY, Li YF, Yu LY, Xie C, Wang HR, Mo W, Zhou HM, Chen S, Liang G, Zhao TJ. Cell Rep. 2019 Jan 2;26(1):209-221.e5. doi: 10.1016/j.celrep.2018.12.022. PubMed
Recessive mutation in CD2AP causes focal segmental glomerulosclerosis in humans and mice. Takano T, Bareke E, Takeda N, Aoudjit L, Baldwin C, Pisano P, Matsuda J, El Andalousi J, Muhtadie L, Bernard C, Majewski J, Miyazaki T, Yamamura KI, Gupta IR. Kidney Int. 2019 Jan;95(1):57-61. doi: 10.1016/j.kint.2018.08.014. Epub 2018 Oct 26. PubMed
An azoospermic factor gene, Ddx3y and its paralog, Ddx3x are dispensable in germ cells for male fertility. Matsumura T, Endo T, Isotani A, Ogawa M, Ikawa M. J Reprod Dev. 2019 Jan 7. doi: 10.1262/jrd.2018-145. PubMed
GADD45A binds R-loops and recruits TET1 to CpG island promoters. Arab K, Karaulanov E, Musheev M, Trnka P, Schafer A, Grummt I, Niehrs C. Nat Genet. 2019 Feb;51(2):217-223. doi: 10.1038/s41588-018-0306-6. Epub 2019 Jan 7. PubMed
Disabling of nephrogenesis in porcine embryos via CRISPR/Cas9-mediated SIX1 and SIX4 gene targeting. Wang J, Liu M, Zhao L, Li Y, Zhang M, Jin Y, Xiong Q, Liu X, Zhang L, Jiang H, Chen Q, Wang C, You Z, Yang H, Cao C, Dai Y, Li R. Xenotransplantation. 2019 Jan 9:e12484. doi: 10.1111/xen.12484. PubMed
Ribosomal protein RPL26 is the principal target of UFMylation. Walczak CP, Leto DE, Zhang L, Riepe C, Muller RY, DaRosa PA, Ingolia NT, Elias JE, Kopito RR. Proc Natl Acad Sci U S A. 2019 Jan 22;116(4):1299-1308. doi: 10.1073/pnas.1816202116. Epub 2019 Jan 9. PubMed
USP15 Participates in Hepatitis C Virus Propagation through Regulation of Viral RNA Translation and Lipid Droplet Formation. Kusakabe S, Suzuki T, Sugiyama Y, Haga S, Horike K, Tokunaga M, Hirano J, Zhang H, Chen DV, Ishiga H, Komoda Y, Ono C, Fukuhara T, Yamamoto M, Ikawa M, Satoh T, Akira S, Tanaka T, Moriishi K, Fukai M, Taketomi A, Yoshio S, Kanto T, Suzuki T, Okamoto T, Matsuura Y. J Virol. 2019 Mar 5;93(6). pii: JVI.01708-18. doi: 10.1128/JVI.01708-18. Print 2019 Mar 15. PubMed
P53 ICE CRIM mouse: a tool to generate mutant allelic series in somatic cells and germ lines for cancer studies. Fan HH, Yu IS, Lin YH, Wang SY, Liaw YH, Chen PL, Yang TL, Lin SW, Chen YT. FASEB J. 2019 Apr;33(4):5571-5584. doi: 10.1096/fj.201802027R. Epub 2019 Jan 14. PubMed
Inhibiting the MCM8-9 complex selectively sensitizes cancer cells to cisplatin and olaparib. Morii I, Iwabuchi Y, Mori S, Suekuni M, Natsume T, Yoshida K, Sugimoto N, Kanemaki MT, Fujita M. Cancer Sci. 2019 Mar;110(3):1044-1053. doi: 10.1111/cas.13941. Epub 2019 Feb 14. PubMed
Galectin-3 Inhibits Cancer Metastasis by Negatively Regulating Integrin beta3 Expression. Hayashi Y, Jia W, Kidoya H, Muramatsu F, Tsukada Y, Takakura N. Am J Pathol. 2019 Apr;189(4):900-910. doi: 10.1016/j.ajpath.2018.12.005. Epub 2019 Jan 14. PubMed
Differential use of E2 ubiquitin conjugating enzymes for regulated degradation of the rate-limiting enzymes HMGCR and SQLE in cholesterol biosynthesis. Tan JME, Cook ECL, van den Berg M, Scheij S, Zelcer N, Loregger A. Atherosclerosis. 2019 Feb;281:137-142. doi: 10.1016/j.atherosclerosis.2018.12.008. Epub 2018 Dec 23. PubMed
Glycerol kinase 2 is essential for proper arrangement of crescent-like mitochondria to form the mitochondrial sheath during mouse spermatogenesis. Shimada K, Kato H, Miyata H, Ikawa M. J Reprod Dev. 2019 Apr 12;65(2):155-162. doi: 10.1262/jrd.2018-136. Epub 2019 Jan 21. PubMed
Structural insights into a high fidelity variant of SpCas9. Guo M, Ren K, Zhu Y, Tang Z, Wang Y, Zhang B, Huang Z. Cell Res. 2019 Mar;29(3):183-192. doi: 10.1038/s41422-018-0131-6. Epub 2019 Jan 21. PubMed
CRISPR/Cas9-mediated deletion of the Wiskott-Aldrich syndrome locus causes actin cytoskeleton disorganization in murine erythroleukemia cells. Fernandez-Calleja V, Fernandez-Nestosa MJ, Hernandez P, Schvartzman JB, Krimer DB. PeerJ. 2019 Jan 16;7:e6284. doi: 10.7717/peerj.6284. eCollection 2019. PubMed
In vivo proximity proteomics of nascent synapses reveals a novel regulator of cytoskeleton-mediated synaptic maturation. Spence EF, Dube S, Uezu A, Locke M, Soderblom EJ, Soderling SH. Nat Commun. 2019 Jan 23;10(1):386. doi: 10.1038/s41467-019-08288-w. PubMed
Involvement of CTCF in transcription regulation of EGR1 at early G1 phase as an architecture factor. Sekiya T, Kato K, Kawaguchi A, Nagata K. Sci Rep. 2019 Jan 23;9(1):329. doi: 10.1038/s41598-018-36753-x. PubMed
Rapid Generation of Long Noncoding RNA Knockout Mice Using CRISPR/Cas9 Technology. Hansmeier NR, Widdershooven PJM, Khani S, Kornfeld JW. Noncoding RNA. 2019 Jan 23;5(1). pii: ncrna5010012. doi: 10.3390/ncrna5010012. PubMed
Targeted depletion of BTF3a in macrophages activates autophagic pathway to eliminate Mycobacterium tuberculosis. Rawat K, Das S, Vivek Vinod BS, Vekariya U, Garg T, Dasgupta A, Tripathi RK. Life Sci. 2019 Mar 1;220:21-31. doi: 10.1016/j.lfs.2019.01.035. Epub 2019 Jan 24. PubMed
E3 Ubiquitin Ligases RNF20 and RNF40 Are Required for Double-Stranded Break (DSB) Repair: Evidence for Monoubiquitination of Histone H2B Lysine 120 as a Novel Axis of DSB Signaling and Repair. So CC, Ramachandran S, Martin A. Mol Cell Biol. 2019 Apr 2;39(8). pii: MCB.00488-18. doi: 10.1128/MCB.00488-18. Print 2019 Apr 15. PubMed
CRMP2 mediates GSK3beta actions in the striatum on regulating neuronal structure and mania-like behavior. Kim W, Won SY, Yoon BJ. J Affect Disord. 2019 Feb 15;245:1079-1088. doi: 10.1016/j.jad.2018.10.371. Epub 2018 Nov 29. PubMed
PKG1-modified TSC2 regulates mTORC1 activity to counter adverse cardiac stress. Ranek MJ, Kokkonen-Simon KM, Chen A, Dunkerly-Eyring BL, Vera MP, Oeing CU, Patel CH, Nakamura T, Zhu G, Bedja D, Sasaki M, Holewinski RJ, Van Eyk JE, Powell JD, Lee DI, Kass DA. Nature. 2019 Feb;566(7743):264-269. doi: 10.1038/s41586-019-0895-y. Epub 2019 Jan 30. PubMed
Population and Single-Cell Analysis of Human Cardiogenesis Reveals Unique LGR5 Ventricular Progenitors in Embryonic Outflow Tract. Sahara M, Santoro F, Sohlmer J, Zhou C, Witman N, Leung CY, Mononen M, Bylund K, Gruber P, Chien KR. Dev Cell. 2019 Feb 25;48(4):475-490.e7. doi: 10.1016/j.devcel.2019.01.005. Epub 2019 Jan 31. PubMed
Oxytocin receptor knockout prairie voles generated by CRISPR/Cas9 editing show reduced preference for social novelty and exaggerated repetitive behaviors. Horie K, Inoue K, Suzuki S, Adachi S, Yada S, Hirayama T, Hidema S, Young LJ, Nishimori K. Horm Behav. 2019 May;111:60-69. doi: 10.1016/j.yhbeh.2018.10.011. Epub 2018 Nov 8. PubMed
CRISPR/Cas9-Mediated Hitchhike Expression of Functional shRNAs at the Porcine miR-17-92 Cluster. Lu C, Pang D, Li M, Yuan H, Yu T, Huang P, Li J, Chen X, Jiao H, Xie Z, Ouyang H. Cells. 2019 Feb 1;8(2). pii: cells8020113. doi: 10.3390/cells8020113. PubMed
AhR controls redox homeostasis and shapes the tumor microenvironment in BRCA1-associated breast cancer. Kubli SP, Bassi C, Roux C, Wakeham A, Gobl C, Zhou W, Jafari SM, Snow B, Jones L, Palomero L, Thu KL, Cassetta L, Soong D, Berger T, Ramachandran P, Baniasadi SP, Duncan G, Lindzen M, Yarden Y, Herranz C, Lazaro C, Chu MF, Haight J, Tinto P, Silvester J, Cescon DW, Petit A, Pettersson S, Pollard JW, Mak TW, Pujana MA, Cappello P, Gorrini C. Proc Natl Acad Sci U S A. 2019 Feb 26;116(9):3604-3613. doi: 10.1073/pnas.1815126116. Epub 2019 Feb 7. PubMed
PUMILIO hyperactivity drives premature aging of Norad-deficient mice. Kopp F, Elguindy MM, Yalvac ME, Zhang H, Chen B, Gillett FA, Lee S, Sivakumar S, Yu H, Xie Y, Mishra P, Sahenk Z, Mendell JT. Elife. 2019 Feb 8;8. pii: 42650. doi: 10.7554/eLife.42650. PubMed
Regulation of Survivin Isoform Expression by GLI Proteins in Ovarian Cancer. Trnski D, Gregoric M, Levanat S, Ozretic P, Rincic N, Vidakovic TM, Kalafatic D, Maurac I, Oreskovic S, Sabol M, Musani V. Cells. 2019 Feb 6;8(2). pii: cells8020128. doi: 10.3390/cells8020128. PubMed
Axis inhibition protein 1 (Axin1) Deletion-Induced Hepatocarcinogenesis Requires Intact beta-Catenin but Not Notch Cascade in Mice. Qiao Y, Wang J, Karagoz E, Liang B, Song X, Shang R, Evert K, Xu M, Che L, Evert M, Calvisi DF, Tao J, Wang B, Monga SP, Chen X. Hepatology. 2019 Feb 8. doi: 10.1002/hep.30556. PubMed
Lvrn expression is not critical for mouse placentation. Tobita T, Kiyozumi D, Muto M, Noda T, Ikawa M. J Reprod Dev. 2019 Jun 14;65(3):239-244. doi: 10.1262/jrd.2018-157. Epub 2019 Feb 10. PubMed
Increased Cytotoxicity of Herpes Simplex Virus Thymidine Kinase Expression in Human Induced Pluripotent Stem Cells. Iwasawa C, Tamura R, Sugiura Y, Suzuki S, Kuzumaki N, Narita M, Suematsu M, Nakamura M, Yoshida K, Toda M, Okano H, Miyoshi H. Int J Mol Sci. 2019 Feb 14;20(4). pii: ijms20040810. doi: 10.3390/ijms20040810. PubMed
Resolving Cell Fate Decisions during Somatic Cell Reprogramming by Single-Cell RNA-Seq. Guo L, Lin L, Wang X, Gao M, Cao S, Mai Y, Wu F, Kuang J, Liu H, Yang J, Chu S, Song H, Li D, Liu Y, Wu K, Liu J, Wang J, Pan G, Hutchins AP, Liu J, Pei D, Chen J. Mol Cell. 2019 Feb 21;73(4):815-829.e7. doi: 10.1016/j.molcel.2019.01.042. Epub 2019 Feb 13. PubMed
Exosome-mediated horizontal gene transfer occurs in double-strand break repair during genome editing. Ono R, Yasuhiko Y, Aisaki KI, Kitajima S, Kanno J, Hirabayashi Y. Commun Biol. 2019 Feb 8;2:57. doi: 10.1038/s42003-019-0300-2. eCollection 2019. PubMed
Targeted editing of the PSIP1 gene encoding LEDGF/p75 protects cells against HIV infection. Lampi Y, Van Looveren D, Vranckx LS, Thiry I, Bornschein S, Debyser Z, Gijsbers R. Sci Rep. 2019 Feb 20;9(1):2389. doi: 10.1038/s41598-019-38718-0. PubMed
Phosphorylation of GATA4 serine 105 but not serine 261 is required for testosterone production in the male mouse. Bergeron F, Boulende Sab A, Bouchard MF, Taniguchi H, Souchkova O, Brousseau C, Tremblay JJ, Pilon N, Viger RS. Andrology. 2019 May;7(3):357-372. doi: 10.1111/andr.12601. Epub 2019 Feb 22. PubMed
The Cep57-pericentrin module organizes PCM expansion and centriole engagement. Watanabe K, Takao D, Ito KK, Takahashi M, Kitagawa D. Nat Commun. 2019 Feb 25;10(1):931. doi: 10.1038/s41467-019-08862-2. PubMed
H4K20me0 recognition by BRCA1-BARD1 directs homologous recombination to sister chromatids. Nakamura K, Saredi G, Becker JR, Foster BM, Nguyen NV, Beyer TE, Cesa LC, Faull PA, Lukauskas S, Frimurer T, Chapman JR, Bartke T, Groth A. Nat Cell Biol. 2019 Mar;21(3):311-318. doi: 10.1038/s41556-019-0282-9. Epub 2019 Feb 25. PubMed
Human Pluripotent Stem Cell-Derived Tumor Model Uncovers the Embryonic Stem Cell Signature as a Key Driver in Atypical Teratoid/Rhabdoid Tumor. Terada Y, Jo N, Arakawa Y, Sakakura M, Yamada Y, Ukai T, Kabata M, Mitsunaga K, Mineharu Y, Ohta S, Nakagawa M, Miyamoto S, Yamamoto T, Yamada Y. Cell Rep. 2019 Mar 5;26(10):2608-2621.e6. doi: 10.1016/j.celrep.2019.02.009. PubMed
Rapid Depletion of DIS3, EXOSC10, or XRN2 Reveals the Immediate Impact of Exoribonucleolysis on Nuclear RNA Metabolism and Transcriptional Control. Davidson L, Francis L, Cordiner RA, Eaton JD, Estell C, Macias S, Caceres JF, West S. Cell Rep. 2019 Mar 5;26(10):2779-2791.e5. doi: 10.1016/j.celrep.2019.02.012. PubMed
Axo-axonic Innervation of Neocortical Pyramidal Neurons by GABAergic Chandelier Cells Requires AnkyrinG-Associated L1CAM. Tai Y, Gallo NB, Wang M, Yu JR, Van Aelst L. Neuron. 2019 Apr 17;102(2):358-372.e9. doi: 10.1016/j.neuron.2019.02.009. Epub 2019 Mar 4. PubMed
Transport of cellular misfolded proteins to the cell surface by HLA-B27 free heavy chain. Yorifuji H, Arase N, Kohyama M, Hirano T, Suenaga T, Kumanogoh A, Arase H. Biochem Biophys Res Commun. 2019 Apr 16;511(4):862-868. doi: 10.1016/j.bbrc.2019.02.120. Epub 2019 Mar 6. PubMed
CRISPR-Cas9 genome editing induces megabase-scale chromosomal truncations. Cullot G, Boutin J, Toutain J, Prat F, Pennamen P, Rooryck C, Teichmann M, Rousseau E, Lamrissi-Garcia I, Guyonnet-Duperat V, Bibeyran A, Lalanne M, Prouzet-Mauleon V, Turcq B, Ged C, Blouin JM, Richard E, Dabernat S, Moreau-Gaudry F, Bedel A. Nat Commun. 2019 Mar 8;10(1):1136. doi: 10.1038/s41467-019-09006-2. PubMed
Efficient Gene Editing at Major CFTR Mutation Loci. Ruan J, Hirai H, Yang D, Ma L, Hou X, Jiang H, Wei H, Rajagopalan C, Mou H, Wang G, Zhang J, Li K, Chen YE, Sun F, Xu J. Mol Ther Nucleic Acids. 2019 Jun 7;16:73-81. doi: 10.1016/j.omtn.2019.02.006. Epub 2019 Feb 16. PubMed
Cytoplasmic TDP-43 De-mixing Independent of Stress Granules Drives Inhibition of Nuclear Import, Loss of Nuclear TDP-43, and Cell Death. Gasset-Rosa F, Lu S, Yu H, Chen C, Melamed Z, Guo L, Shorter J, Da Cruz S, Cleveland DW. Neuron. 2019 Apr 17;102(2):339-357.e7. doi: 10.1016/j.neuron.2019.02.038. Epub 2019 Mar 7. PubMed
The Chromatin Structure of CRISPR-Cas9 Target DNA Controls the Balance between Mutagenic and Homology-Directed Gene-Editing Events. Janssen JM, Chen X, Liu J, Goncalves MAFV. Mol Ther Nucleic Acids. 2019 Jun 7;16:141-154. doi: 10.1016/j.omtn.2019.02.009. Epub 2019 Feb 20. PubMed
Establishment of gene-edited pigs expressing human blood-coagulation factor VII and albumin for bioartificial liver use. Li L, Meng H, Zou Q, Zhang J, Cai L, Yang B, Weng J, Lai L, Yang H, Gao Y. J Gastroenterol Hepatol. 2019 Mar 18. doi: 10.1111/jgh.14666. PubMed
Inhibition of proteasome rescues a pathogenic variant of respiratory chain assembly factor COA7. Mohanraj K, Wasilewski M, Beninca C, Cysewski D, Poznanski J, Sakowska P, Bugajska Z, Deckers M, Dennerlein S, Fernandez-Vizarra E, Rehling P, Dadlez M, Zeviani M, Chacinska A. EMBO Mol Med. 2019 May;11(5). pii: emmm.201809561. doi: 10.15252/emmm.201809561. PubMed
Ubiquitin-specific protease 26 (USP26) is not essential for mouse gametogenesis and fertility. Felipe-Medina N, Gomez-H L, Condezo YB, Sanchez-Martin M, Barbero JL, Ramos I, Llano E, Pendas AM. Chromosoma. 2019 Mar 18. pii: 10.1007/s00412-019-00697-6. doi: 10.1007/s00412-019-00697-6. PubMed
Human Genomic Safe Harbors and the Suicide Gene-Based Safeguard System for iPSC-Based Cell Therapy. Kimura Y, Shofuda T, Higuchi Y, Nagamori I, Oda M, Nakamori M, Onodera M, Kanematsu D, Yamamoto A, Katsuma A, Suemizu H, Nakano T, Kanemura Y, Mochizuki H. Stem Cells Transl Med. 2019 Jul;8(7):627-638. doi: 10.1002/sctm.18-0039. Epub 2019 Mar 19. PubMed
Staphylococcal Superantigens Stimulate Epithelial Cells through CD40 To Produce Chemokines. Schlievert PM, Cahill MP, Hostager BS, Brosnahan AJ, Klingelhutz AJ, Gourronc FA, Bishop GA, Leung DYM. MBio. 2019 Mar 19;10(2). pii: mBio.00214-19. doi: 10.1128/mBio.00214-19. PubMed
MDC1 Interacts with TOPBP1 to Maintain Chromosomal Stability during Mitosis. Leimbacher PA, Jones SE, Shorrocks AK, de Marco Zompit M, Day M, Blaauwendraad J, Bundschuh D, Bonham S, Fischer R, Fink D, Kessler BM, Oliver AW, Pearl LH, Blackford AN, Stucki M. Mol Cell. 2019 May 2;74(3):571-583.e8. doi: 10.1016/j.molcel.2019.02.014. Epub 2019 Mar 18. PubMed
Development of a Selective CDK7 Covalent Inhibitor Reveals Predominant Cell-Cycle Phenotype. Olson CM, Liang Y, Leggett A, Park WD, Li L, Mills CE, Elsarrag SZ, Ficarro SB, Zhang T, Duster R, Geyer M, Sim T, Marto JA, Sorger PK, Westover KD, Lin CY, Kwiatkowski N, Gray NS. Cell Chem Biol. 2019 Jun 20;26(6):792-803.e10. doi: 10.1016/j.chembiol.2019.02.012. Epub 2019 Mar 21. PubMed
In Vivo Editing of the Adult Mouse Liver Using CRISPR/Cas9 and Hydrodynamic Tail Vein Injection. Niola F, Dagnaes-Hansen F, Frodin M. Methods Mol Biol. 2019;1961:329-341. doi: 10.1007/978-1-4939-9170-9_20. PubMed
CtIP contributes to non-homologous end joining formation through interacting with ligase IV and promotion of TMZ resistance in glioma cells. Yang B, Han N, Sun J, Jiang H, Xu HY. Eur Rev Med Pharmacol Sci. 2019 Mar;23(5):2092-2102. doi: 10.26355/eurrev_201903_17252. PubMed
HDAC1 negatively regulates selective mitotic chromatin binding of the Notch effector RBPJ in a KDM5A-dependent manner. Dreval K, Lake RJ, Fan HY. Nucleic Acids Res. 2019 May 21;47(9):4521-4538. doi: 10.1093/nar/gkz178. PubMed
Mice with Calr mutations homologous to human CALR mutations only exhibit mild thrombocytosis. Shide K, Kameda T, Kamiunten A, Oji A, Ozono Y, Sekine M, Honda A, Kitanaka A, Akizuki K, Tahira Y, Nakamura K, Hidaka T, Kubuki Y, Abe H, Miike T, Iwakiri H, Tahara Y, Sueta M, Hasuike S, Yamamoto S, Nagata K, Ikawa M, Shimoda K. Blood Cancer J. 2019 Mar 29;9(4):42. doi: 10.1038/s41408-019-0202-z. PubMed
Guidelines for Fluorescent Guided Biallelic HDR Targeting Selection With PiggyBac System Removal for Gene Editing. Jarazo J, Qing X, Schwamborn JC. Front Genet. 2019 Mar 13;10:190. doi: 10.3389/fgene.2019.00190. eCollection 2019. PubMed
CRISPR screening using an expanded toolkit of autophagy reporters identifies TMEM41B as a novel autophagy factor. Shoemaker CJ, Huang TQ, Weir NR, Polyakov NJ, Schultz SW, Denic V. PLoS Biol. 2019 Apr 1;17(4):e2007044. doi: 10.1371/journal.pbio.2007044. eCollection 2019 Apr. PubMed
Nonhomologous end joining key factor XLF enhances both 5-florouracil and oxaliplatin resistance in colorectal cancer. Liu Z, Yu M, Fei B, Sun J, Wang D. Onco Targets Ther. 2019 Mar 20;12:2095-2104. doi: 10.2147/OTT.S192923. eCollection 2019. PubMed
Optimizing genome editing strategy by primer-extension-mediated sequencing. Yin J, Liu M, Liu Y, Wu J, Gan T, Zhang W, Li Y, Zhou Y, Hu J. Cell Discov. 2019 Mar 26;5:18. doi: 10.1038/s41421-019-0088-8. eCollection 2019. PubMed
PtdIns3P phosphatases MTMR3 and MTMR4 negatively regulate innate immune responses to DNA through modulating STING trafficking. Dewi Pamungkas Putri D, Kawasaki T, Murase M, Sueyoshi T, Deguchi T, Ori D, Suetsugu S, Kawai T. J Biol Chem. 2019 May 24;294(21):8412-8423. doi: 10.1074/jbc.RA118.005731. Epub 2019 Apr 3. PubMed
Gene signatures of quiescent glioblastoma cells reveal mesenchymal shift and interactions with niche microenvironment. Tejero R, Huang Y, Katsyv I, Kluge M, Lin JY, Tome-Garcia J, Daviaud N, Wang Y, Zhang B, Tsankova NM, Friedel CC, Zou H, Friedel RH. EBioMedicine. 2019 Apr 2. pii: S2352-3964(19)30209-9. doi: 10.1016/j.ebiom.2019.03.064. PubMed Associated Plasmids pAAVS1-Neo-CAG-M2rtTA-H2BGFP (donor AB) pAAVS1-Neo-CAG-M2rtTA-H2BmNeon (donorAB-mNeon) pAAVS1-Neo-UBC-M2rtTA-H2BmNeon (donorAB-UBC-mNeon) pX330-sgAAVS1
Autophagy regulates lipid metabolism through selective turnover of NCoR1. Saito T, Kuma A, Sugiura Y, Ichimura Y, Obata M, Kitamura H, Okuda S, Lee HC, Ikeda K, Kanegae Y, Saito I, Auwerx J, Motohashi H, Suematsu M, Soga T, Yokomizo T, Waguri S, Mizushima N, Komatsu M. Nat Commun. 2019 Apr 5;10(1):1567. doi: 10.1038/s41467-019-08829-3. PubMed
TMCO1-mediated Ca(2+) leak underlies osteoblast functions via CaMKII signaling. Li J, Liu C, Li Y, Zheng Q, Xu Y, Liu B, Sun W, Li Y, Ji S, Liu M, Zhang J, Zhao D, Du R, Liu Z, Zhong G, Sun C, Wang Y, Song J, Zhang S, Qin J, Ling S, Wang X, Li Y. Nat Commun. 2019 Apr 8;10(1):1589. doi: 10.1038/s41467-019-09653-5. PubMed
Gene correction for SCID-X1 in long-term hematopoietic stem cells. Pavel-Dinu M, Wiebking V, Dejene BT, Srifa W, Mantri S, Nicolas CE, Lee C, Bao G, Kildebeck EJ, Punjya N, Sindhu C, Inlay MA, Saxena N, DeRavin SS, Malech H, Roncarolo MG, Weinberg KI, Porteus MH. Nat Commun. 2019 Apr 9;10(1):1634. doi: 10.1038/s41467-019-09614-y. PubMed
A Bioluminescence Reporter Assay for Retinoic Acid Control of Translation of the GluR1 Subunit of the AMPA Glutamate Receptor. Khatib T, Whiting A, Chisholm DR, Redfern C, Muller B, McCaffery P. Mol Neurobiol. 2019 Apr 10. pii: 10.1007/s12035-019-1571-9. doi: 10.1007/s12035-019-1571-9. PubMed
An Adversarial DNA N(6)-Methyladenine-Sensor Network Preserves Polycomb Silencing. Kweon SM, Chen Y, Moon E, Kvederaviciute K, Klimasauskas S, Feldman DE. Mol Cell. 2019 Apr 5. pii: S1097-2765(19)30222-9. doi: 10.1016/j.molcel.2019.03.018. PubMed Associated Plasmids p3XFlag-Mettl4 p3xFlag-Alkbh4 hASXL(1-115)-luciferase pET30a-ASXL1-HARE-HTH domain pET30a-RAMA More ...
CRISPR-SONIC: targeted somatic oncogene knock-in enables rapid in vivo cancer modeling. Mou H, Ozata DM, Smith JL, Sheel A, Kwan SY, Hough S, Kucukural A, Kennedy Z, Cao Y, Xue W. Genome Med. 2019 Apr 16;11(1):21. doi: 10.1186/s13073-019-0627-9. PubMed Associated Plasmids H513 px330.sgActin.UTR.2 H517 SONIC HRASV12 donor H516 SONIC KrasG12D-IRES-luciferase donor
An atypical LIR motif within UBA5 (ubiquitin like modifier activating enzyme 5) interacts with GABARAP proteins and mediates membrane localization of UBA5. Huber J, Obata M, Gruber J, Akutsu M, Lohr F, Rogova N, Guntert P, Dikic I, Kirkin V, Komatsu M, Dotsch V, Rogov VV. Autophagy. 2020 Feb;16(2):256-270. doi: 10.1080/15548627.2019.1606637. Epub 2019 Apr 28. PubMed
In utero gene editing for monogenic lung disease. Alapati D, Zacharias WJ, Hartman HA, Rossidis AC, Stratigis JD, Ahn NJ, Coons B, Zhou S, Li H, Singh K, Katzen J, Tomer Y, Chadwick AC, Musunuru K, Beers MF, Morrisey EE, Peranteau WH. Sci Transl Med. 2019 Apr 17;11(488). pii: 11/488/eaav8375. doi: 10.1126/scitranslmed.aav8375. PubMed
Dysregulation of humoral immunity in Foxp3 conditional-knockout mice. Tai Y, Sakamoto K, Takano A, Haga K, Harada Y. Biochem Biophys Res Commun. 2019 Jun 11;513(4):787-793. doi: 10.1016/j.bbrc.2019.04.090. Epub 2019 Apr 15. PubMed
In vivo genome editing rescues photoreceptor degeneration via a Cas9/RecA-mediated homology-directed repair pathway. Cai Y, Cheng T, Yao Y, Li X, Ma Y, Li L, Zhao H, Bao J, Zhang M, Qiu Z, Xue T. Sci Adv. 2019 Apr 17;5(4):eaav3335. doi: 10.1126/sciadv.aav3335. eCollection 2019 Apr. PubMed
Boosting activity of high-fidelity CRISPR/Cas9 variants using a tRNA(Gln)-processing system in human cells. He X, Wang Y, Yang F, Wang B, Xie H, Gu L, Zhao T, Liu X, Zhang D, Ren Q, Liu X, Liu Y, Gao C, Gu F. J Biol Chem. 2019 Jun 7;294(23):9308-9315. doi: 10.1074/jbc.RA119.007791. Epub 2019 Apr 22. PubMed
Multifunctional CRISPR-Cas9 with engineered immunosilenced human T cell epitopes. Ferdosi SR, Ewaisha R, Moghadam F, Krishna S, Park JG, Ebrahimkhani MR, Kiani S, Anderson KS. Nat Commun. 2019 Apr 23;10(1):1842. doi: 10.1038/s41467-019-09693-x. PubMed
Single-cell transcriptomes of the regenerating intestine reveal a revival stem cell. Ayyaz A, Kumar S, Sangiorgi B, Ghoshal B, Gosio J, Ouladan S, Fink M, Barutcu S, Trcka D, Shen J, Chan K, Wrana JL, Gregorieff A. Nature. 2019 May;569(7754):121-125. doi: 10.1038/s41586-019-1154-y. Epub 2019 Apr 24. PubMed
CRISPR-Cas9-Mediated Correction of the 1.02 kb Common Deletion in CLN3 in Induced Pluripotent Stem Cells from Patients with Batten Disease. Burnight ER, Bohrer LR, Giacalone JC, Klaahsen DL, Daggett HT, East JS, Madumba RA, Worthington KS, Mullins RF, Stone EM, Tucker BA, Wiley LA. CRISPR J. 2018 Feb;1:75-87. doi: 10.1089/crispr.2017.0015. PubMed
Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Yesbolatova A, Natsume T, Hayashi KI, Kanemaki MT. Methods. 2019 Apr 23. pii: S1046-2023(18)30331-1. doi: 10.1016/j.ymeth.2019.04.010. PubMed Associated Plasmids pMK344 (Hygro-P2A-mAID) pMK345 (Hygro-P2A-mAID-mClover) pMK346 (Hygro-P2A-mAID-mCherry) pMK347 (BSD-P2A-mAID) pMK348 (BSD-P2A-mAID-mClover) More ...
Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts. Benchetrit H, Jaber M, Zayat V, Sebban S, Pushett A, Makedonski K, Zakheim Z, Radwan A, Maoz N, Lasry R, Renous N, Inbar M, Ram O, Kaplan T, Buganim Y. Cell Stem Cell. 2019 Jun 6;24(6):983-994.e7. doi: 10.1016/j.stem.2019.03.018. Epub 2019 Apr 25. PubMed Associated Plasmids FUW-TetO-Gata6 FUW-TetO-Sox17 FUW-TetO-Cdx2 FUW-TetO-Klf5 FUW-TetO-Sox15 More ...
Genome Editing in Mammalian Cell Lines using CRISPR-Cas. Liu KI, Sutrisnoh NB, Wang Y, Tan MH. J Vis Exp. 2019 Apr 11;(146). doi: 10.3791/59086. PubMed
Whole body glucoregulation and tissue-specific glucose uptake in a novel Akt substrate of 160 kDa knockout rat model. Arias EB, Zheng X, Agrawal S, Cartee GD. PLoS One. 2019 Apr 29;14(4):e0216236. doi: 10.1371/journal.pone.0216236. eCollection 2019. PubMed
A novel human stem cell model for Coffin-Siris Syndrome like syndrome reveals the importance of SOX11 dosage for neuronal differentiation and survival. Turan S, Boerstler T, Kavyanifar A, Loskarn S, Reis A, Winner B, Lie DC. Hum Mol Genet. 2019 Apr 29. pii: 5481587. doi: 10.1093/hmg/ddz089. PubMed
Effect of transcription inhibition and generation of suppressive viral non-coding RNAs. Pinto DO, Scott TA, DeMarino C, Pleet ML, Vo TT, Saifuddin M, Kovalskyy D, Erickson J, Cowen M, Barclay RA, Zeng C, Weinberg MS, Kashanchi F. Retrovirology. 2019 Apr 29;16(1):13. doi: 10.1186/s12977-019-0475-0. PubMed
Mammalian SWI/SNF collaborates with a polycomb-associated protein to regulate male germline transcription in the mouse. Menon DU, Shibata Y, Mu W, Magnuson T. Development. 2019 Jul 5;146(19). pii: dev.174094. doi: 10.1242/dev.174094. PubMed
Cross-Regulation between TDP-43 and Paraspeckles Promotes Pluripotency-Differentiation Transition. Modic M, Grosch M, Rot G, Schirge S, Lepko T, Yamazaki T, Lee FCY, Rusha E, Shaposhnikov D, Palo M, Merl-Pham J, Cacchiarelli D, Rogelj B, Hauck SM, von Mering C, Meissner A, Lickert H, Hirose T, Ule J, Drukker M. Mol Cell. 2019 Jun 6;74(5):951-965.e13. doi: 10.1016/j.molcel.2019.03.041. Epub 2019 Apr 29. PubMed
sgRNA-shRNA Structure Mediated SNP Site Editing on Porcine IGF2 Gene by CRISPR/StCas9. Sun Y, Yan N, Mu L, Sun B, Deng J, Fang Y, Shao S, Yan Q, Han F, Zhang Z, Xu K. Front Genet. 2019 Apr 18;10:347. doi: 10.3389/fgene.2019.00347. eCollection 2019. PubMed
CRISPR/Cas9 System for Efficient Genome Editing and Targeting in the Mouse NIH/3T3 Cells. Mehravar M, Shirazi A, Mehrazar MM, Nazari M, Banan M. Avicenna J Med Biotechnol. 2019 Apr-Jun;11(2):149-155. PubMed
The transcription factor CBFB suppresses breast cancer through orchestrating translation and transcription. Malik N, Yan H, Moshkovich N, Palangat M, Yang H, Sanchez V, Cai Z, Peat TJ, Jiang S, Liu C, Lee M, Mock BA, Yuspa SH, Larson D, Wakefield LM, Huang J. Nat Commun. 2019 May 6;10(1):2071. doi: 10.1038/s41467-019-10102-6. PubMed
Caspase-1 initiates apoptosis in the absence of gasdermin D. Tsuchiya K, Nakajima S, Hosojima S, Thi Nguyen D, Hattori T, Manh Le T, Hori O, Mahib MR, Yamaguchi Y, Miura M, Kinoshita T, Kushiyama H, Sakurai M, Shiroishi T, Suda T. Nat Commun. 2019 May 7;10(1):2091. doi: 10.1038/s41467-019-09753-2. PubMed
Dlg1 activates beta-catenin signaling to regulate retinal angiogenesis and the blood-retina and blood-brain barriers. Cho C, Wang Y, Smallwood PM, Williams J, Nathans J. Elife. 2019 May 8;8. pii: 45542. doi: 10.7554/eLife.45542. PubMed
Zfp281 Shapes the Transcriptome of Trophoblast Stem Cells and Is Essential for Placental Development. Ishiuchi T, Ohishi H, Sato T, Kamimura S, Yorino M, Abe S, Suzuki A, Wakayama T, Suyama M, Sasaki H. Cell Rep. 2019 May 7;27(6):1742-1754.e6. doi: 10.1016/j.celrep.2019.04.028. PubMed
Genome Editing in Zebrafish Using CRISPR-Cas9: Applications for Developmental Toxicology. Warner BK, Alder JK, Suli A. Methods Mol Biol. 2019;1965:235-250. doi: 10.1007/978-1-4939-9182-2_16. PubMed
The COMMD3/8 complex determines GRK6 specificity for chemoattractant receptors. Nakai A, Fujimoto J, Miyata H, Stumm R, Narazaki M, Schulz S, Baba Y, Kumanogoh A, Suzuki K. J Exp Med. 2019 Jul 1;216(7):1630-1647. doi: 10.1084/jem.20181494. Epub 2019 May 14. PubMed
Absence of integrin alpha3beta1 promotes the progression of HER2-driven breast cancer in vivo. Ramovs V, Secades P, Song JY, Thijssen B, Kreft M, Sonnenberg A. Breast Cancer Res. 2019 May 17;21(1):63. doi: 10.1186/s13058-019-1146-8. PubMed
A piggyBac-based toolkit for inducible genome editing in mammalian cells. Schertzer MD, Thulson E, Braceros KCA, Lee DM, Hinkle ER, Murphy RM, Kim SO, Vitucci EM, Calabrese JM. RNA. 2019 May 17. pii: rna.068932.118. doi: 10.1261/rna.068932.118. PubMed Associated Plasmids PB_rtTA_BsmBI PB_tre_Cas9 PB_tre_dCas9_KRAB PB_tre_dCas9_VP160
Functional rare and low frequency variants in BLK and BANK1 contribute to human lupus. Jiang SH, Athanasopoulos V, Ellyard JI, Chuah A, Cappello J, Cook A, Prabhu SB, Cardenas J, Gu J, Stanley M, Roco JA, Papa I, Yabas M, Walters GD, Burgio G, McKeon K, Byers JM, Burrin C, Enders A, Miosge LA, Canete PF, Jelusic M, Tasic V, Lungu AC, Alexander SI, Kitching AR, Fulcher DA, Shen N, Arsov T, Gatenby PA, Babon JJ, Mallon DF, de Lucas Collantes C, Stone EA, Wu P, Field MA, Andrews TD, Cho E, Pascual V, Cook MC, Vinuesa CG. Nat Commun. 2019 May 17;10(1):2201. doi: 10.1038/s41467-019-10242-9. PubMed
Intracellular Ca(2+) Homeostasis and Nuclear Export Mediate Exit from Naive Pluripotency. MacDougall MS, Clarke R, Merrill BJ. Cell Stem Cell. 2019 Aug 1;25(2):210-224.e6. doi: 10.1016/j.stem.2019.04.015. Epub 2019 May 16. PubMed
Enhancement of Precise Gene Editing by the Association of Cas9 With Homologous Recombination Factors. Tran NT, Bashir S, Li X, Rossius J, Chu VT, Rajewsky K, Kuhn R. Front Genet. 2019 Apr 30;10:365. doi: 10.3389/fgene.2019.00365. eCollection 2019. PubMed
Generation of cattle knockout for galactose-alpha1,3-galactose and N-glycolylneuraminic acid antigens. Perota A, Lagutina I, Duchi R, Zanfrini E, Lazzari G, Judor JP, Conchon S, Bach JM, Bottio T, Gerosa G, Costa C, Galinanes M, Roussel JC, Padler-Karavani V, Cozzi E, Soulillou JP, Galli C. Xenotransplantation. 2019 May 22:e12524. doi: 10.1111/xen.12524. PubMed
Possible detrimental effects of beta-2-microglobulin knockout in pigs. Sake HJ, Frenzel A, Lucas-Hahn A, Nowak-Imialek M, Hassel P, Hadeler KG, Hermann D, Becker R, Eylers H, Hein R, Baars W, Brinkmann A, Schwinzer R, Niemann H, Petersen B. Xenotransplantation. 2019 May 22:e12525. doi: 10.1111/xen.12525. PubMed
Kinesin-14 motor protein KIFC1 participates in DNA synthesis and chromatin maintenance. Wei YL, Yang WX. Cell Death Dis. 2019 May 24;10(6):402. doi: 10.1038/s41419-019-1619-9. PubMed
Slc12a8 is a nicotinamide mononucleotide transporter. Grozio A, Mills KF, Yoshino J, Bruzzone S, Sociali G, Tokizane K, Lei HC, Cunningham R, Sasaki Y, Migaud ME, Imai SI. Nat Metab. 2019 Jan;1(1):47-57. doi: 10.1038/s42255-018-0009-4. Epub 2019 Jan 7. PubMed
LINGO-1 deficiency promotes nerve regeneration through reduction of cell apoptosis, inflammation, and glial scar after spinal cord injury in mice. Huang LJ, Li G, Ding Y, Sun JH, Wu TT, Zhao W, Zeng YS. Exp Neurol. 2019 May 25;320:112965. doi: 10.1016/j.expneurol.2019.112965. PubMed
The bipartite TAD organization of the X-inactivation center ensures opposing developmental regulation of Tsix and Xist. van Bemmel JG, Galupa R, Gard C, Servant N, Picard C, Davies J, Szempruch AJ, Zhan Y, Zylicz JJ, Nora EP, Lameiras S, de Wit E, Gentien D, Baulande S, Giorgetti L, Guttman M, Hughes JR, Higgs DR, Gribnau J, Heard E. Nat Genet. 2019 Jun;51(6):1024-1034. doi: 10.1038/s41588-019-0412-0. Epub 2019 May 27. PubMed
Aminopeptidase N Knockout Pigs Are Not Resistant to Porcine Epidemic Diarrhea Virus Infection. Zhang J, Wu Z, Yang H. Virol Sin. 2019 May 27. pii: 10.1007/s12250-019-00127-y. doi: 10.1007/s12250-019-00127-y. PubMed
Cytotoxicity of trifluridine correlates with the thymidine kinase 1 expression level. Kataoka Y, Iimori M, Niimi S, Tsukihara H, Wakasa T, Saeki H, Oki E, Maehara Y, Kitao H. Sci Rep. 2019 May 28;9(1):7964. doi: 10.1038/s41598-019-44399-6. PubMed
Lysolipid receptor cross-talk regulates lymphatic endothelial junctions in lymph nodes. Hisano Y, Kono M, Cartier A, Engelbrecht E, Kano K, Kawakami K, Xiong Y, Piao W, Galvani S, Yanagida K, Kuo A, Ono Y, Ishida S, Aoki J, Proia RL, Bromberg JS, Inoue A, Hla T. J Exp Med. 2019 Jul 1;216(7):1582-1598. doi: 10.1084/jem.20181895. Epub 2019 May 30. PubMed
Highly efficient editing of the beta-globin gene in patient-derived hematopoietic stem and progenitor cells to treat sickle cell disease. Park SH, Lee CM, Dever DP, Davis TH, Camarena J, Srifa W, Zhang Y, Paikari A, Chang AK, Porteus MH, Sheehan VA, Bao G. Nucleic Acids Res. 2019 Sep 5;47(15):7955-7972. doi: 10.1093/nar/gkz475. PubMed
A knock-in mouse model of Pendred syndrome with Slc26a4 L236P mutation. Wen Z, Zhu H, Li Z, Zhang S, Zhang A, Zhang T, Fu X, Sun D, Zhang J, Gao J. Biochem Biophys Res Commun. 2019 Jul 23;515(2):359-365. doi: 10.1016/j.bbrc.2019.05.157. Epub 2019 May 30. PubMed
Phosphorylation of TET2 by AMPK is indispensable in myogenic differentiation. Zhang T, Guan X, Choi UL, Dong Q, Lam MMT, Zeng J, Xiong J, Wang X, Poon TCW, Zhang H, Zhang X, Wang H, Xie R, Zhu B, Li G. Epigenetics Chromatin. 2019 Jun 4;12(1):32. doi: 10.1186/s13072-019-0281-x. PubMed
Impaired telomere integrity and rRNA biogenesis in PARN-deficient patients and knock-out models. Benyelles M, Episkopou H, O'Donohue MF, Kermasson L, Frange P, Poulain F, Burcu Belen F, Polat M, Bole-Feysot C, Langa-Vives F, Gleizes PE, de Villartay JP, Callebaut I, Decottignies A, Revy P. EMBO Mol Med. 2019 Jun 6. pii: emmm.201810201. doi: 10.15252/emmm.201810201. PubMed
A Human Embryonic Stem Cell Reporter Line for Monitoring Chemical-induced Cardiotoxicity. Tsai SY, Ghazizadeh Z, Wang HJ, Ortega FA, Badieyan ZS, Hsu ZT, Gordillo M, Kumar R, Christini DJ, Evans T, Chen S. Cardiovasc Res. 2019 Jun 7. pii: 5512499. doi: 10.1093/cvr/cvz148. PubMed
Compensatory mechanisms in myoglobin deficient mice preserve NO homeostasis. Park JW, Piknova B, Dey S, Noguchi CT, Schechter AN. Nitric Oxide. 2019 Sep 1;90:10-14. doi: 10.1016/j.niox.2019.06.001. Epub 2019 Jun 4. PubMed
beta-catenin activation promotes immune escape and resistance to anti-PD-1 therapy in hepatocellular carcinoma. Ruiz de Galarreta M, Bresnahan E, Molina-Sanchez P, Lindblad KE, Maier B, Sia D, Puigvehi M, Miguela V, Casanova-Acebes M, Dhainaut M, Villacorta-Martin C, Singhi AD, Moghe A, von Felden J, Tal Grinspan L, Wang S, Kamphorst AO, Monga SP, Brown BD, Villanueva A, Llovet JM, Merad M, Lujambio A. Cancer Discov. 2019 Jun 11. pii: 2159-8290.CD-19-0074. doi: 10.1158/2159-8290.CD-19-0074. PubMed Associated Plasmids pT3-EF1A-MYC-IRES-luc pT3-EF1A-MYC-IRES-lucOS
Regulation of the Hepatitis B virus replication and gene expression by the multi-functional protein TARDBP. Makokha GN, Abe-Chayama H, Chowdhury S, Hayes CN, Tsuge M, Yoshima T, Ishida Y, Zhang Y, Uchida T, Tateno C, Akiyama R, Chayama K. Sci Rep. 2019 Jun 11;9(1):8462. doi: 10.1038/s41598-019-44934-5. PubMed
Quantitative Interactomics in Primary T Cells Provides a Rationale for Concomitant PD-1 and BTLA Coinhibitor Blockade in Cancer Immunotherapy. Celis-Gutierrez J, Blattmann P, Zhai Y, Jarmuzynski N, Ruminski K, Gregoire C, Ounoughene Y, Fiore F, Aebersold R, Roncagalli R, Gstaiger M, Malissen B. Cell Rep. 2019 Jun 11;27(11):3315-3330.e7. doi: 10.1016/j.celrep.2019.05.041. PubMed
Generation of bicistronic reporter knockin mice for visualizing germ layers. Suzuki H, Dinh TTH, Daitoku Y, Tanimoto Y, Kato K, Azami T, Ema M, Murata K, Mizuno S, Sugiyama F. Exp Anim. 2019 Jun 13. doi: 10.1538/expanim.19-0031. PubMed
CRISPR/Cas9-mediated in vivo gene editing reveals that neuronal 5-HT1A receptors in the dorsal raphe nucleus contribute to body temperature regulation in mice. Nishitani N, Ohmura Y, Nagayasu K, Shibui N, Kaneko S, Ohashi A, Yoshida T, Yamanaka A, Yoshioka M. Brain Res. 2019 Sep 15;1719:243-252. doi: 10.1016/j.brainres.2019.06.009. Epub 2019 Jun 10. PubMed
Identification of a genomic enhancer that enforces proper apoptosis induction in thymic negative selection. Hojo MA, Masuda K, Hojo H, Nagahata Y, Yasuda K, Ohara D, Takeuchi Y, Hirota K, Suzuki Y, Kawamoto H, Kawaoka S. Nat Commun. 2019 Jun 13;10(1):2603. doi: 10.1038/s41467-019-10525-1. PubMed
Loss of autophagy in chondrocytes causes severe growth retardation. Horigome Y, Ida-Yonemochi H, Waguri S, Shibata S, Endo N, Komatsu M. Autophagy. 2020 Mar;16(3):501-511. doi: 10.1080/15548627.2019.1628541. Epub 2019 Jun 16. PubMed
Modeling of Fibrotic Lung Disease Using 3D Organoids Derived from Human Pluripotent Stem Cells. Strikoudis A, Cieslak A, Loffredo L, Chen YW, Patel N, Saqi A, Lederer DJ, Snoeck HW. Cell Rep. 2019 Jun 18;27(12):3709-3723.e5. doi: 10.1016/j.celrep.2019.05.077. PubMed
Generation of FOS gene knockout lines from a human embryonic stem cell line using CRISPR/Cas9. Li C, Wang Q, Peng Z, Lin Y, Liu H, Yang X, Li S, Liu X, Chen J. Stem Cell Res. 2019 Jun 5;39:101479. doi: 10.1016/j.scr.2019.101479. PubMed
Down-regulation of ATF1 leads to early neuroectoderm differentiation of human embryonic stem cells by increasing the expression level of SOX2. Yang SC, Liu JJ, Wang CK, Lin YT, Tsai SY, Chen WJ, Huang WK, Tu PA, Lin YC, Chang CF, Cheng CL, Lin H, Lai CY, Lin CY, Lee YH, Chiu YC, Hsu CC, Hsu SC, Hsiao M, Schuyler SC, Lu FL, Lu J. FASEB J. 2019 Jun 26:fj201800220RR. doi: 10.1096/fj.201800220RR. PubMed
miR-29 family regulates the puberty onset mediated by a novel Gnrh1 transcription factor TBX21. Li X, Xiao J, Fan Y, Yang K, Li K, Wang X, Lu Y, Zhou Y. J Endocrinol. 2019 Jun 1. pii: JOE-19-0082.R2. doi: 10.1530/JOE-19-0082. PubMed
High-throughput identification of human SNPs affecting regulatory element activity. van Arensbergen J, Pagie L, FitzPatrick VD, de Haas M, Baltissen MP, Comoglio F, van der Weide RH, Teunissen H, Vosa U, Franke L, de Wit E, Vermeulen M, Bussemaker HJ, van Steensel B. Nat Genet. 2019 Jul;51(7):1160-1169. doi: 10.1038/s41588-019-0455-2. Epub 2019 Jun 28. PubMed
lncRNA-Induced Spread of Polycomb Controlled by Genome Architecture, RNA Abundance, and CpG Island DNA. Schertzer MD, Braceros KCA, Starmer J, Cherney RE, Lee DM, Salazar G, Justice M, Bischoff SR, Cowley DO, Ariel P, Zylka MJ, Dowen JM, Magnuson T, Calabrese JM. Mol Cell. 2019 Aug 8;75(3):523-537.e10. doi: 10.1016/j.molcel.2019.05.028. Epub 2019 Jun 27. PubMed Associated Plasmids PB_rtTA_BsmB-HNRNPK_sgRNA_1 PB_rtTA_BsmB-HNRNPK_sgRNA_3 PB_rtTA_BsmB-HNRNPK_sgRNA_4
ssODN-Mediated In-Frame Deletion with CRISPR/Cas9 Restores FVIII Function in Hemophilia A-Patient-Derived iPSCs and ECs. Hu Z, Zhou M, Wu Y, Li Z, Liu X, Wu L, Liang D. Mol Ther Nucleic Acids. 2019 Jun 5;17:198-209. doi: 10.1016/j.omtn.2019.05.019. PubMed
Missense Mutations in the Human Nanophthalmos Gene TMEM98 Cause Retinal Defects in the Mouse. Cross SH, Mckie L, Keighren M, West K, Thaung C, Davey T, Soares DC, Sanchez-Pulido L, Jackson IJ. Invest Ophthalmol Vis Sci. 2019 Jul 1;60(8):2875-2887. doi: 10.1167/iovs.18-25954. PubMed
E2F4 regulates transcriptional activation in mouse embryonic stem cells independently of the RB family. Hsu J, Arand J, Chaikovsky A, Mooney NA, Demeter J, Brison CM, Oliverio R, Vogel H, Rubin SM, Jackson PK, Sage J. Nat Commun. 2019 Jul 3;10(1):2939. doi: 10.1038/s41467-019-10901-x. PubMed
Reduced PAX2 expression in murine fallopian tube cells enhances estrogen receptor signaling. Colina J, Varughese P, Karthikeyan S, Salvi A, Modi D, Burdette JE. Carcinogenesis. 2019 Jul 4. pii: 5528129. doi: 10.1093/carcin/bgz127. PubMed
Impaired telomere integrity and rRNA biogenesis in PARN-deficient patients and knock-out models. Benyelles M, Episkopou H, O'Donohue MF, Kermasson L, Frange P, Poulain F, Burcu Belen F, Polat M, Bole-Feysot C, Langa-Vives F, Gleizes PE, de Villartay JP, Callebaut I, Decottignies A, Revy P. EMBO Mol Med. 2019 Jul;11(7):e10201. doi: 10.15252/emmm.201810201. Epub 2019 Jun 6. PubMed
Circulating miR-338 Cluster activities on osteoblast differentiation: Potential Diagnostic and Therapeutic Targets for Postmenopausal Osteoporosis. Lin C, Yu S, Jin R, Xiao Y, Pan M, Pei F, Zhu X, Huang H, Zhang Z, Chen S, Liu H, Chen Z. Theranostics. 2019 May 31;9(13):3780-3797. doi: 10.7150/thno.34493. eCollection 2019. PubMed
Mammalian NSUN2 introduces 5-methylcytidines into mitochondrial tRNAs. Shinoda S, Kitagawa S, Nakagawa S, Wei FY, Tomizawa K, Araki K, Araki M, Suzuki T, Suzuki T. Nucleic Acids Res. 2019 Sep 19;47(16):8734-8745. doi: 10.1093/nar/gkz575. PubMed
Potential limitations of the Sleeping Beauty transposon use in gene expression studies. Sowinska W, Wawro M, Solecka A, Kasza A. Acta Biochim Pol. 2019 Jul 12;66(3):263-268. doi: 10.18388/abp.2019_2839. PubMed
The Cytoplasmic DNA Sensor cGAS Promotes Mitotic Cell Death. Zierhut C, Yamaguchi N, Paredes M, Luo JD, Carroll T, Funabiki H. Cell. 2019 Jul 11;178(2):302-315.e23. doi: 10.1016/j.cell.2019.05.035. PubMed
A Chemical Screen Identifies Compounds Capable of Selecting for Haploidy in Mammalian Cells. Olbrich T, Vega-Sendino M, Murga M, de Carcer G, Malumbres M, Ortega S, Ruiz S, Fernandez-Capetillo O. Cell Rep. 2019 Jul 16;28(3):597-604.e4. doi: 10.1016/j.celrep.2019.06.060. PubMed
Lysophosphatidic acid-induced YAP/TAZ activation promotes developmental angiogenesis by repressing Notch ligand Dll4. Yasuda D, Kobayashi D, Akahoshi N, Ohto-Nakanishi T, Yoshioka K, Takuwa Y, Mizuno S, Takahashi S, Ishii S. J Clin Invest. 2019 Jul 23;130:4332-4349. doi: 10.1172/JCI121955. PubMed
Glucocorticoid receptor quaternary structure drives chromatin occupancy and transcriptional outcome. Paakinaho V, Johnson TA, Presman DM, Hager GL. Genome Res. 2019 Aug;29(8):1223-1234. doi: 10.1101/gr.244814.118. Epub 2019 Jul 23. PubMed
Establishment of knockout adult sea urchins by using a CRISPR-Cas9 system. Liu D, Awazu A, Sakuma T, Yamamoto T, Sakamoto N. Dev Growth Differ. 2019 Aug;61(6):378-388. doi: 10.1111/dgd.12624. Epub 2019 Jul 29. PubMed
Stimulation of CRISPR-mediated homology-directed repair by an engineered RAD18 variant. Nambiar TS, Billon P, Diedenhofen G, Hayward SB, Taglialatela A, Cai K, Huang JW, Leuzzi G, Cuella-Martin R, Palacios A, Gupta A, Egli D, Ciccia A. Nat Commun. 2019 Jul 30;10(1):3395. doi: 10.1038/s41467-019-11105-z. PubMed Associated Plasmids pFUGW-GFP-2-cut reporter pcDNA3.1(+)- e18 pcDNA3.1(+)- e18 LOF
Heterozygous Mutations in SMARCA2 Reprogram the Enhancer Landscape by Global Retargeting of SMARCA4. Gao F, Elliott NJ, Ho J, Sharp A, Shokhirev MN, Hargreaves DC. Mol Cell. 2019 Sep 5;75(5):891-904.e7. doi: 10.1016/j.molcel.2019.06.024. Epub 2019 Jul 30. PubMed
Distinct roles of RAD52 and POLQ in chromosomal break repair and replication stress response. Kelso AA, Lopezcolorado FW, Bhargava R, Stark JM. PLoS Genet. 2019 Aug 5;15(8):e1008319. doi: 10.1371/journal.pgen.1008319. eCollection 2019 Aug. PubMed
Optimized CRISPR/Cas9-mediated in vivo genome engineering applicable to monitoring dynamics of endogenous proteins in the mouse neural tissues. Matsuda T, Oinuma I. Sci Rep. 2019 Aug 5;9(1):11309. doi: 10.1038/s41598-019-47721-4. PubMed
beta-Catenin/Tcf7l2-dependent transcriptional regulation of GLUT1 gene expression by Zic family proteins in colon cancer. Zhao Z, Wang L, Bartom E, Marshall S, Rendleman E, Ryan C, Shilati A, Savas J, Chandel N, Shilatifard A. Sci Adv. 2019 Jul 31;5(7):eaax0698. doi: 10.1126/sciadv.aax0698. eCollection 2019 Jul. PubMed
Unlimited Genetic Switches for Cell-Type-Specific Manipulation. Garcia-Marques J, Yang CP, Espinosa-Medina I, Mok K, Koyama M, Lee T. Neuron. 2019 Oct 23;104(2):227-238.e7. doi: 10.1016/j.neuron.2019.07.005. Epub 2019 Aug 5. PubMed
Nested retrotransposition in the East Asian mouse genome causes the classical nonagouti mutation. Tanave A, Imai Y, Koide T. Commun Biol. 2019 Aug 2;2:283. doi: 10.1038/s42003-019-0539-7. eCollection 2019. PubMed
A Pliable Mediator Acts as a Functional Rather Than an Architectural Bridge between Promoters and Enhancers. El Khattabi L, Zhao H, Kalchschmidt J, Young N, Jung S, Van Blerkom P, Kieffer-Kwon P, Kieffer-Kwon KR, Park S, Wang X, Krebs J, Tripathi S, Sakabe N, Sobreira DR, Huang SC, Rao SSP, Pruett N, Chauss D, Sadler E, Lopez A, Nobrega MA, Aiden EL, Asturias FJ, Casellas R. Cell. 2019 Aug 22;178(5):1145-1158.e20. doi: 10.1016/j.cell.2019.07.011. Epub 2019 Aug 8. PubMed
The endosomal sorting adaptor HD-PTP is required for ephrin-B:EphB signalling in cellular collapse and spinal motor axon guidance. Lahaie S, Morales D, Bagci H, Hamoud N, Castonguay CE, Kazan JM, Desrochers G, Klar A, Gingras AC, Pause A, Cote JF, Kania A. Sci Rep. 2019 Aug 16;9(1):11945. doi: 10.1038/s41598-019-48421-9. PubMed
Bi-allelic GOT2 Mutations Cause a Treatable Malate-Aspartate Shuttle-Related Encephalopathy. van Karnebeek CDM, Ramos RJ, Wen XY, Tarailo-Graovac M, Gleeson JG, Skrypnyk C, Brand-Arzamendi K, Karbassi F, Issa MY, van der Lee R, Drogemoller BI, Koster J, Rousseau J, Campeau PM, Wang Y, Cao F, Li M, Ruiter J, Ciapaite J, Kluijtmans LAJ, Willemsen MAAP, Jans JJ, Ross CJ, Wintjes LT, Rodenburg RJ, Huigen MCDG, Jia Z, Waterham HR, Wasserman WW, Wanders RJA, Verhoeven-Duif NM, Zaki MS, Wevers RA. Am J Hum Genet. 2019 Sep 5;105(3):534-548. doi: 10.1016/j.ajhg.2019.07.015. Epub 2019 Aug 15. PubMed
The Barrier Molecules Junction Plakoglobin, Filaggrin, and Dystonin Play Roles in Melanoma Growth and Angiogenesis. Leick KM, Rodriguez AB, Melssen MM, Benamar M, Lindsay RS, Eki R, Du KP, Parlak M, Abbas T, Engelhard VH, Slingluff CL Jr. Ann Surg. 2019 Oct;270(4):712-722. doi: 10.1097/SLA.0000000000003522. PubMed
A transient reporter for editing enrichment (TREE) in human cells. Standage-Beier K, Tekel SJ, Brookhouser N, Schwarz G, Nguyen T, Wang X, Brafman DA. Nucleic Acids Res. 2019 Aug 20. pii: 5552066. doi: 10.1093/nar/gkz713. PubMed Associated Plasmids pEF-BE4Gam pEF-AncBE4max pDT-sgRNA pEF-BFP
Agonist-induced membrane nanodomain clustering drives GLP-1 receptor responses in pancreatic beta cells. Buenaventura T, Bitsi S, Laughlin WE, Burgoyne T, Lyu Z, Oqua AI, Norman H, McGlone ER, Klymchenko AS, Correa IR Jr, Walker A, Inoue A, Hanyaloglu A, Grimes J, Koszegi Z, Calebiro D, Rutter GA, Bloom SR, Jones B, Tomas A. PLoS Biol. 2019 Aug 20;17(8):e3000097. doi: 10.1371/journal.pbio.3000097. eCollection 2019 Aug. PubMed
The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility. Gomez-H L, Felipe-Medina N, Condezo YB, Garcia-Valiente R, Ramos I, Suja JA, Barbero JL, Roig I, Sanchez-Martin M, de Rooij DG, Llano E, Pendas AM. PLoS Genet. 2019 Aug 22;15(8):e1008316. doi: 10.1371/journal.pgen.1008316. eCollection 2019 Aug. PubMed
Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation. Gurumurthy CB, O'Brien AR, Quadros RM, Adams J Jr, Alcaide P, Ayabe S, Ballard J, Batra SK, Beauchamp MC, Becker KA, Bernas G, Brough D, Carrillo-Salinas F, Chan W, Chen H, Dawson R, DeMambro V, D'Hont J, Dibb KM, Eudy JD, Gan L, Gao J, Gonzales A, Guntur AR, Guo H, Harms DW, Harrington A, Hentges KE, Humphreys N, Imai S, Ishii H, Iwama M, Jonasch E, Karolak M, Keavney B, Khin NC, Konno M, Kotani Y, Kunihiro Y, Lakshmanan I, Larochelle C, Lawrence CB, Li L, Lindner V, Liu XD, Lopez-Castejon G, Loudon A, Lowe J, Jerome-Majewska LA, Matsusaka T, Miura H, Miyasaka Y, Morpurgo B, Motyl K, Nabeshima YI, Nakade K, Nakashiba T, Nakashima K, Obata Y, Ogiwara S, Ouellet M, Oxburgh L, Piltz S, Pinz I, Ponnusamy MP, Ray D, Redder RJ, Rosen CJ, Ross N, Ruhe MT, Ryzhova L, Salvador AM, Alam SS, Sedlacek R, Sharma K, Smith C, Staes K, Starrs L, Sugiyama F, Takahashi S, Tanaka T, Trafford AW, Uno Y, Vanhoutte L, Vanrockeghem F, Willis BJ, Wright CS, Yamauchi Y, Yi X, Yoshimi K, Zhang X, Zhang Y, Ohtsuka M, Das S, Garry DJ, Hochepied T, Thomas P, Parker-Thornburg J, Adamson AD, Yoshiki A, Schmouth JF, Golovko A, Thompson WR, Lloyd KCK, Wood JA, Cowan M, Mashimo T, Mizuno S, Zhu H, Kasparek P, Liaw L, Miano JM, Burgio G. Genome Biol. 2019 Aug 26;20(1):171. doi: 10.1186/s13059-019-1776-2. PubMed
Knock-In Rat Lines with Cre Recombinase at the Dopamine D1 and Adenosine 2a Receptor Loci. Pettibone JR, Yu JY, Derman RC, Faust TW, Hughes ED, Filipiak WE, Saunders TL, Ferrario CR, Berke JD. eNeuro. 2019 Oct 3;6(5). pii: ENEURO.0163-19.2019. doi: 10.1523/ENEURO.0163-19.2019. Print 2019 Sep/Oct. PubMed
A versatile toolbox for knock-in gene targeting based on the Multisite Gateway technology. Yoshimatsu S, Sone T, Nakajima M, Sato T, Okochi R, Ishikawa M, Nakamura M, Sasaki E, Shiozawa S, Okano H. PLoS One. 2019 Aug 27;14(8):e0221164. doi: 10.1371/journal.pone.0221164. eCollection 2019. PubMed Associated Plasmids pENTR-L1-PGK-PuroTK-L2 pENTR-L1-PGK-HygTK-L2 pENTR-L1-PGK-Neo-L2 pENTR-L1-PGK-TKNeo-L2 pENTR-L1-PGK-TKBsd-L2 More ...
Efficient Production of Biallelic RAG1 Knockout Mouse Embryonic Stem Cell Using CRISPR/Cas9. Mehravar M, Shirazi A, Mehrazar MM, Nazari M, Banan M, Salimi M. Iran J Biotechnol. 2019 Jan 11;17(1):e2205. doi: 10.21859/ijb.2205. eCollection 2019 Jan. PubMed
GREB1 induced by Wnt signaling promotes development of hepatoblastoma by suppressing TGFbeta signaling. Matsumoto S, Yamamichi T, Shinzawa K, Kasahara Y, Nojima S, Kodama T, Obika S, Takehara T, Morii E, Okuyama H, Kikuchi A. Nat Commun. 2019 Aug 28;10(1):3882. doi: 10.1038/s41467-019-11533-x. PubMed
Glaucoma-Associated Mutations in the Optineurin Gene Have Limited Impact on Parkin-Dependent Mitophagy. Chernyshova K, Inoue K, Yamashita SI, Fukuchi T, Kanki T. Invest Ophthalmol Vis Sci. 2019 Aug 1;60(10):3625-3635. doi: 10.1167/iovs.19-27184. PubMed
Identification of Glyceraldehyde-3-Phosphate Dehydrogenase Gene as an Alternative Safe Harbor Locus in Pig Genome. Han X, Xiong Y, Zhao C, Xie S, Li C, Li X, Liu X, Li K, Zhao S, Ruan J. Genes (Basel). 2019 Aug 29;10(9). pii: genes10090660. doi: 10.3390/genes10090660. PubMed
Knock-in of murine Calr del52 induces essential thrombocythemia with slow-rising dominance in mice and reveals key role of Calr exon 9 in cardiac development. Balligand T, Achouri Y, Pecquet C, Gaudray G, Colau D, Hug E, Rahmani Y, Stroobant V, Plo I, Vainchenker W, Kralovics R, Van den Eynde BJ, Defour JP, Constantinescu SN. Leukemia. 2020 Feb;34(2):510-521. doi: 10.1038/s41375-019-0538-1. Epub 2019 Aug 30. PubMed
Regulation of histone methylation by automethylation of PRC2. Wang X, Long Y, Paucek RD, Gooding AR, Lee T, Burdorf RM, Cech TR. Genes Dev. 2019 Oct 1;33(19-20):1416-1427. doi: 10.1101/gad.328849.119. Epub 2019 Sep 5. PubMed
Single Amino Acid Change Underlies Distinct Roles of H2A.Z Subtypes in Human Syndrome. Greenberg RS, Long HK, Swigut T, Wysocka J. Cell. 2019 Sep 5;178(6):1421-1436.e24. doi: 10.1016/j.cell.2019.08.002. PubMed
Human RECQL4 represses the RAD52-mediated single-strand annealing pathway after ionizing radiation or cisplatin treatment. Kohzaki M, Ootsuyama A, Sun L, Moritake T, Okazaki R. Int J Cancer. 2020 Jun 1;146(11):3098-3113. doi: 10.1002/ijc.32670. Epub 2019 Oct 6. PubMed
Iterative, multiplexed CRISPR-mediated gene editing for functional analysis of complex protease gene clusters. Callies LK, Tadeo D, Simper J, Bugge TH, Szabo R. J Biol Chem. 2019 Nov 1;294(44):15987-15996. doi: 10.1074/jbc.RA119.009773. Epub 2019 Sep 9. PubMed
Fast and cloning-free CRISPR/Cas9-mediated genomic editing in mammalian cells. Manna PT, Davis LJ, Robinson MS. Traffic. 2019 Dec;20(12):974-982. doi: 10.1111/tra.12696. Epub 2019 Oct 17. PubMed
Cooperative action of APJ and alpha1A-adrenergic receptor in vascular smooth muscle cells induces vasoconstriction. Nagano K, Kwon C, Ishida J, Hashimoto T, Kim JD, Kishikawa N, Murao M, Kimura K, Kasuya Y, Kimura S, Chen YC, Tsuchimochi H, Shirai M, Pearson JT, Fukamizu A. J Biochem. 2019 Nov 1;166(5):383-392. doi: 10.1093/jb/mvz071. PubMed
Imaging endogenous synaptic proteins in primary neurons at single-cell resolution using CRISPR/Cas9. Matsuda T, Oinuma I. Mol Biol Cell. 2019 Oct 15;30(22):2838-2855. doi: 10.1091/mbc.E19-04-0223. Epub 2019 Sep 11. PubMed
Fascin Controls Metastatic Colonization and Mitochondrial Oxidative Phosphorylation by Remodeling Mitochondrial Actin Filaments. Lin S, Huang C, Gunda V, Sun J, Chellappan SP, Li Z, Izumi V, Fang B, Koomen J, Singh PK, Hao J, Yang S. Cell Rep. 2019 Sep 10;28(11):2824-2836.e8. doi: 10.1016/j.celrep.2019.08.011. PubMed
Homology-directed repair in mouse cells increased by CasRx-mediated knockdown or co-expressing Kaposi's sarcoma-associated herpesvirus ORF52. Pan H, Yu W, Zhang M. Biosci Rep. 2019 Oct 30;39(10). pii: BSR20191914. doi: 10.1042/BSR20191914. PubMed
Deletion of CD163 Exon 7 Confers Resistance to Highly Pathogenic Porcine Reproductive and Respiratory Viruses on Pigs. Wang H, Shen L, Chen J, Liu X, Tan T, Hu Y, Bai X, Li Y, Tian K, Li N, Hu X. Int J Biol Sci. 2019 Jul 25;15(9):1993-2005. doi: 10.7150/ijbs.34269. eCollection 2019. PubMed
Hepatitis C Virus Infection Is Inhibited by a Noncanonical Antiviral Signaling Pathway Targeted by NS3-NS4A. Vazquez C, Tan CY, Horner SM. J Virol. 2019 Nov 13;93(23). pii: JVI.00725-19. doi: 10.1128/JVI.00725-19. Print 2019 Dec 1. PubMed
Ran pathway-independent regulation of mitotic Golgi disassembly by Importin-alpha. Chang CC, Chen CJ, Grauffel C, Pien YC, Lim C, Tsai SY, Hsia KC. Nat Commun. 2019 Sep 20;10(1):4307. doi: 10.1038/s41467-019-12207-4. PubMed
NHEJ-Mediated Repair of CRISPR-Cas9-Induced DNA Breaks Efficiently Corrects Mutations in HSPCs from Patients with Fanconi Anemia. Roman-Rodriguez FJ, Ugalde L, Alvarez L, Diez B, Ramirez MJ, Risueno C, Corton M, Bogliolo M, Bernal S, March F, Ayuso C, Hanenberg H, Sevilla J, Rodriguez-Perales S, Torres-Ruiz R, Surralles J, Bueren JA, Rio P. Cell Stem Cell. 2019 Nov 7;25(5):607-621.e7. doi: 10.1016/j.stem.2019.08.016. Epub 2019 Sep 19. PubMed
Extracellular ADP augments microglial inflammasome and NF-kappaB activation via the P2Y12 receptor. Suzuki T, Kohyama K, Moriyama K, Ozaki M, Hasegawa S, Ueno T, Saitoe M, Morio T, Hayashi M, Sakuma H. Eur J Immunol. 2020 Feb;50(2):205-219. doi: 10.1002/eji.201848013. Epub 2019 Oct 2. PubMed
A mechanism in agrin signaling revealed by a prevalent Rapsyn mutation in congenital myasthenic syndrome. Xing G, Jing H, Zhang L, Cao Y, Li L, Zhao K, Dong Z, Chen W, Wang H, Cao R, Xiong WC, Mei L. Elife. 2019 Sep 24;8. pii: 49180. doi: 10.7554/eLife.49180. PubMed
Phospho-Ser727 triggers a multistep inactivation of STAT3 by rapid dissociation of pY705-SH2 through C-terminal tail modulation. Yang J, Kunimoto H, Katayama B, Zhao H, Shiromizu T, Wang L, Ozawa T, Tomonaga T, Tsuruta D, Nakajima K. Int Immunol. 2020 Feb 7;32(2):73-88. doi: 10.1093/intimm/dxz061. PubMed
Delineating the role of MITF isoforms in pigmentation and tissue homeostasis. Flesher JL, Paterson-Coleman EK, Vasudeva P, Ruiz-Vega R, Marshall M, Pearlman E, MacGregor GR, Neumann J, Ganesan AK. Pigment Cell Melanoma Res. 2020 Mar;33(2):279-292. doi: 10.1111/pcmr.12828. Epub 2019 Oct 16. PubMed
NEIL1 and NEIL2 DNA glycosylases protect neural crest development against mitochondrial oxidative stress. Han D, Schomacher L, Schule KM, Mallick M, Musheev MU, Karaulanov E, Krebs L, von Seggern A, Niehrs C. Elife. 2019 Sep 30;8. pii: 49044. doi: 10.7554/eLife.49044. PubMed
Unique and redundant roles of SOX2 and SOX17 in regulating the germ cell tumor fate. Jostes SV, Fellermeyer M, Arevalo L, Merges GE, Kristiansen G, Nettersheim D, Schorle H. Int J Cancer. 2020 Mar 15;146(6):1592-1605. doi: 10.1002/ijc.32714. Epub 2019 Nov 1. PubMed
Interleukin 22 disrupts pancreatic function in newborn mice expressing IL-23. Chen L, Strohmeier V, He Z, Deshpande M, Catalan-Dibene J, Durum SK, Moran TM, Kraus T, Xiong H, Faith JJ, Sodhi CP, Hackam DJ, Lira SA, Furtado GC. Nat Commun. 2019 Oct 4;10(1):4517. doi: 10.1038/s41467-019-12540-8. PubMed
Viable pigs after simultaneous inactivation of porcine MHC class I and three xenoreactive antigen genes GGTA1, CMAH and B4GALNT2. Fischer K, Rieblinger B, Hein R, Sfriso R, Zuber J, Fischer A, Klinger B, Liang W, Flisikowski K, Kurome M, Zakhartchenko V, Kessler B, Wolf E, Rieben R, Schwinzer R, Kind A, Schnieke A. Xenotransplantation. 2020 Jan;27(1):e12560. doi: 10.1111/xen.12560. Epub 2019 Oct 8. PubMed
Targeted knock-in into the OVA locus of chicken cells using CRISPR/Cas9 system with homology-independent targeted integration. Shi M, Kawabe Y, Ito A, Kamihira M. J Biosci Bioeng. 2019 Oct 5. pii: S1389-1723(19)30665-6. doi: 10.1016/j.jbiosc.2019.09.011. PubMed
Recruitment of DNA Repair MRN Complex by Intrinsically Disordered Protein Domain Fused to Cas9 Improves Efficiency of CRISPR-Mediated Genome Editing. Reuven N, Adler J, Broennimann K, Myers N, Shaul Y. Biomolecules. 2019 Oct 8;9(10). pii: biom9100584. doi: 10.3390/biom9100584. PubMed Associated Plasmids pU6-(BsaI)_CBh-Cas9 pU6-(BsaI)_CBh-UN-Cas9 pU6-(BsaI)_CBh-Cas9-T2A-mCherry pU6-(BsaI)_CBh-UN-Cas9-T2A-mCherry pU6-(BsaI)_CBh-UC-Cas9-T2A-mCherry
CRISPR/Cas9-Based Genome Editing of HSV. Velusamy T, Gowripalan A, Tscharke DC. Methods Mol Biol. 2020;2060:169-183. doi: 10.1007/978-1-4939-9814-2_9. PubMed
PRMT6 Promotes Lung Tumor Progression via the Alternate Activation of Tumor-Associated Macrophages. Avasarala S, Wu PY, Khan SQ, Yanlin S, Van Scoyk M, Bao J, Di Lorenzo A, David O, Bedford MT, Gupta V, Winn RA, Bikkavilli RK. Mol Cancer Res. 2020 Jan;18(1):166-178. doi: 10.1158/1541-7786.MCR-19-0204. Epub 2019 Oct 16. PubMed
Chromosome Engineering of Human Colon-Derived Organoids to Develop a Model of Traditional Serrated Adenoma. Kawasaki K, Fujii M, Sugimoto S, Ishikawa K, Matano M, Ohta Y, Toshimitsu K, Takahashi S, Hosoe N, Sekine S, Kanai T, Sato T. Gastroenterology. 2020 Feb;158(3):638-651.e8. doi: 10.1053/j.gastro.2019.10.009. Epub 2019 Oct 14. PubMed
Distinct Roles for Condensin's Two ATPase Sites in Chromosome Condensation. Elbatsh AMO, Kim E, Eeftens JM, Raaijmakers JA, van der Weide RH, Garcia-Nieto A, Bravo S, Ganji M, Uit de Bos J, Teunissen H, Medema RH, de Wit E, Haering CH, Dekker C, Rowland BD. Mol Cell. 2019 Dec 5;76(5):724-737.e5. doi: 10.1016/j.molcel.2019.09.020. Epub 2019 Oct 16. PubMed
ZP4 confers structural properties to the zona pellucida essential for embryo development. Lamas-Toranzo I, Fonseca Balvis N, Querejeta-Fernandez A, Izquierdo-Rico MJ, Gonzalez-Brusi L, Lorenzo PL, Garcia-Rebollar P, Aviles M, Bermejo-Alvarez P. Elife. 2019 Oct 22;8. pii: 48904. doi: 10.7554/eLife.48904. PubMed
Targetable cellular signaling events mediate vascular pathology in vascular Ehlers-Danlos syndrome. Bowen CJ, Calderon Giadrosic JF, Burger Z, Rykiel G, Davis EC, Helmers MR, Benke K, Gallo MacFarlane E, Dietz HC. J Clin Invest. 2020 Feb 3;130(2):686-698. doi: 10.1172/JCI130730. PubMed
Regulation of bile acid metabolism in mouse models with hydrophobic bile acid composition. Honda A, Miyazaki T, Iwamoto J, Hirayama T, Morishita Y, Monma T, Ueda H, Mizuno S, Sugiyama F, Takahashi S, Ikegami T. J Lipid Res. 2020 Jan;61(1):54-69. doi: 10.1194/jlr.RA119000395. Epub 2019 Oct 23. PubMed
The apparent deglycase activity of DJ-1 results from the conversion of free methylglyoxal present in fast equilibrium with hemithioacetals and hemiaminals. Andreeva A, Bekkhozhin Z, Omertassova N, Baizhumanov T, Yeltay G, Akhmetali M, Toibazar D, Utepbergenov D. J Biol Chem. 2019 Dec 6;294(49):18863-18872. doi: 10.1074/jbc.RA119.011237. Epub 2019 Oct 24. PubMed
Preparation of a new type 2 diabetic miniature pig model via the CRISPR/Cas9 system. Zou X, Ouyang H, Yu T, Chen X, Pang D, Tang X, Chen C. Cell Death Dis. 2019 Oct 28;10(11):823. doi: 10.1038/s41419-019-2056-5. PubMed
Caspase-8, receptor-interacting protein kinase 1 (RIPK1), and RIPK3 regulate retinoic acid-induced cell differentiation and necroptosis. Someda M, Kuroki S, Miyachi H, Tachibana M, Yonehara S. Cell Death Differ. 2019 Oct 28. pii: 10.1038/s41418-019-0434-2. doi: 10.1038/s41418-019-0434-2. PubMed
Fundamental roles of chromatin loop extrusion in antibody class switching. Zhang X, Zhang Y, Ba Z, Kyritsis N, Casellas R, Alt FW. Nature. 2019 Nov;575(7782):385-389. doi: 10.1038/s41586-019-1723-0. Epub 2019 Oct 30. PubMed
PML isoform expression and DNA break location relative to PML nuclear bodies impacts the efficiency of homologous recombination. Attwood KM, Salsman J, Chung D, Mathavarajah S, Van Iderstine C, Dellaire G. Biochem Cell Biol. 2019 Oct 31. doi: 10.1139/bcb-2019-0115. PubMed
Phosphorylation of TRPV1 S801 Contributes to Modality-Specific Hyperalgesia in Mice. Joseph J, Qu L, Wang S, Kim M, Bennett D, Ro J, Caterina MJ, Chung MK. J Neurosci. 2019 Dec 11;39(50):9954-9966. doi: 10.1523/JNEUROSCI.1064-19.2019. Epub 2019 Nov 1. PubMed
LSD1-mediated repression of GFI1 super-enhancer plays an essential role in erythroleukemia. Tatsumi G, Kawahara M, Yamamoto R, Hishizawa M, Kito K, Suzuki T, Takaori-Kondo A, Andoh A. Leukemia. 2019 Nov 1. pii: 10.1038/s41375-019-0614-6. doi: 10.1038/s41375-019-0614-6. PubMed
Control of RNA Pol II Speed by PNUTS-PP1 and Spt5 Dephosphorylation Facilitates Termination by a "Sitting Duck Torpedo" Mechanism. Cortazar MA, Sheridan RM, Erickson B, Fong N, Glover-Cutter K, Brannan K, Bentley DL. Mol Cell. 2019 Dec 19;76(6):896-908.e4. doi: 10.1016/j.molcel.2019.09.031. Epub 2019 Oct 30. PubMed
Caspase-7 mediates caspase-1-induced apoptosis independently of Bid. Mahib MR, Hosojima S, Kushiyama H, Kinoshita T, Shiroishi T, Suda T, Tsuchiya K. Microbiol Immunol. 2020 Feb;64(2):143-152. doi: 10.1111/1348-0421.12756. Epub 2019 Nov 20. PubMed
Natural HIV-1 Nef Polymorphisms Impair SERINC5 Downregulation Activity. Jin SW, Alsahafi N, Kuang XT, Swann SA, Toyoda M, Gottlinger H, Walker BD, Ueno T, Finzi A, Brumme ZL, Brockman MA. Cell Rep. 2019 Nov 5;29(6):1449-1457.e5. doi: 10.1016/j.celrep.2019.10.007. PubMed
Aryl Sulfonamides Degrade RBM39 and RBM23 by Recruitment to CRL4-DCAF15. Ting TC, Goralski M, Klein K, Wang B, Kim J, Xie Y, Nijhawan D. Cell Rep. 2019 Nov 5;29(6):1499-1510.e6. doi: 10.1016/j.celrep.2019.09.079. PubMed
Generating Mutant Renal Cell Lines Using CRISPR Technologies. Perretta-Tejedor N, Freke G, Seda M, Long DA, Jenkins D. Methods Mol Biol. 2020;2067:323-340. doi: 10.1007/978-1-4939-9841-8_20. PubMed
Efficient cleavage resolves PAM preferences of CRISPR-Cas in human cells. Tang L, Yang F, He X, Xie H, Liu X, Fu J, Xi H, Lu X, Liu C, Song Z, Qu J, Zhao J, Gu F. Cell Regen (Lond). 2019 Oct 28;8(2):44-50. doi: 10.1016/j.cr.2019.08.002. eCollection 2019 Dec. PubMed
Anti-tumor effects of an antagonistic mAb against the ASCT2 amino acid transporter on KRAS-mutated human colorectal cancer cells. Hara Y, Minami Y, Yoshimoto S, Hayashi N, Yamasaki A, Ueda S, Masuko K, Masuko T. Cancer Med. 2020 Jan;9(1):302-312. doi: 10.1002/cam4.2689. Epub 2019 Nov 10. PubMed
Marker-less deletion of cctA gene of Clostridium chauvoei. Gupta SK, Dixit S, Dangi SK, Kaur G, Mashooq M, Karthik K, Sarkar M, Mahajan S, Nagaleekar VK. Anaerobe. 2019 Nov 8;61:102116. doi: 10.1016/j.anaerobe.2019.102116. PubMed
The Axonal Membrane Protein PRG2 Inhibits PTEN and Directs Growth to Branches. Brosig A, Fuchs J, Ipek F, Kroon C, Schrotter S, Vadhvani M, Polyzou A, Ledderose J, van Diepen M, Holzhutter HG, Trimbuch T, Gimber N, Schmoranzer J, Lieberam I, Rosenmund C, Spahn C, Scheerer P, Szczepek M, Leondaritis G, Eickholt BJ. Cell Rep. 2019 Nov 12;29(7):2028-2040.e8. doi: 10.1016/j.celrep.2019.10.039. PubMed
CRISPR-based modular assembly of a UAS-cDNA/ORF plasmid library for more than 5500 Drosophila genes conserved in humans. Wei P, Xue W, Zhao Y, Ning G, Wang J. Genome Res. 2020 Jan;30(1):95-106. doi: 10.1101/gr.250811.119. Epub 2019 Nov 13. PubMed
Non-invasive in vivo imaging of UCP1 expression in live mice via near-infrared fluorescent protein iRFP720. Fukuda A, Honda S, Fujioka N, Sekiguchi Y, Mizuno S, Miwa Y, Sugiyama F, Hayashi Y, Nishimura K, Hisatake K. PLoS One. 2019 Nov 15;14(11):e0225213. doi: 10.1371/journal.pone.0225213. eCollection 2019. PubMed
Chicken MBD4 Regulates Immunoglobulin Diversification by Somatic Hypermutation. Costello R, Cantillo JF, Kenter AL. Front Immunol. 2019 Nov 1;10:2540. doi: 10.3389/fimmu.2019.02540. eCollection 2019. PubMed
A super enhancer controls expression and chromatin architecture within the MHC class II locus. Majumder P, Lee JT, Rahmberg AR, Kumar G, Mi T, Scharer CD, Boss JM. J Exp Med. 2020 Feb 3;217(2). pii: jem.20190668. doi: 10.1084/jem.20190668. PubMed
The canonical non-homologous end joining factor XLF promotes chromosomal deletion rearrangements in human cells. Bhargava R, Lopezcolorado FW, Tsai LJ, Stark JM. J Biol Chem. 2019 Nov 21. pii: RA119.010421. doi: 10.1074/jbc.RA119.010421. PubMed Associated Plasmids px330-CD4 px330-GAPDH
Dominant negative Gfi1b mutations cause moderate thrombocytopenia and an impaired stress thrombopoiesis associated with mild erythropoietic abnormalities in mice. Beauchemin H, Shooshtharizadeh P, Pinder J, Dellaire G, Moroy T. Haematologica. 2019 Nov 21. pii: haematol.2019.222596. doi: 10.3324/haematol.2019.222596. PubMed
DAPK1 loss triggers tumor invasion in colorectal tumor cells. Steinmann S, Kunze P, Hampel C, Eckstein M, Bertram Bramsen J, Muenzner JK, Carle B, Ndreshkjana B, Kemenes S, Gasparini P, Friedrich O, Andersen C, Geppert C, Wang S, Eyupoglu I, Bauerle T, Hartmann A, Schneider-Stock R. Cell Death Dis. 2019 Nov 26;10(12):895. doi: 10.1038/s41419-019-2122-z. PubMed
Temporal Control of the TGF-beta Signaling Network by Mouse ESC MicroRNA Targets of Different Affinities. Kelly TJ, Brummer A, Hooshdaran N, Tariveranmoshabad M, Zamudio JR. Cell Rep. 2019 Nov 26;29(9):2702-2717.e7. doi: 10.1016/j.celrep.2019.10.109. PubMed
Uncovering the role of MAFB in glucagon production and secretion in pancreatic alpha-cells using a new alpha-cell-specific Mafb conditional knockout mouse model. Chang YH, Katoh MC, Abdellatif AM, Xiafukaiti G, Elzeftawy A, Ojima M, Mizuno S, Kuno A, Takahashi S. Exp Anim. 2019 Dec 2. doi: 10.1538/expanim.19-0105. PubMed
Biogenesis and functions of aminocarboxypropyluridine in tRNA. Takakura M, Ishiguro K, Akichika S, Miyauchi K, Suzuki T. Nat Commun. 2019 Dec 5;10(1):5542. doi: 10.1038/s41467-019-13525-3. PubMed
A unified allosteric/torpedo mechanism for transcriptional termination on human protein-coding genes. Eaton JD, Francis L, Davidson L, West S. Genes Dev. 2020 Jan 1;34(1-2):132-145. doi: 10.1101/gad.332833.119. Epub 2019 Dec 5. PubMed
Highly efficient CRISPR-targeting of the murine Hipp11 intergenic region supports inducible human transgene expression. Browning J, Rooney M, Hams E, Takahashi S, Mizuno S, Sugiyama F, Fallon PG, Kelly VP. Mol Biol Rep. 2020 Feb;47(2):1491-1498. doi: 10.1007/s11033-019-05204-9. Epub 2019 Dec 6. PubMed
CRISPR/Cas9-Mediated Integration of Large Transgene into Pig CEP112 Locus. Li G, Zhang X, Wang H, Mo J, Zhong C, Shi J, Zhou R, Li Z, Yang H, Wu Z, Liu D. G3 (Bethesda). 2020 Feb 6;10(2):467-473. doi: 10.1534/g3.119.400810. PubMed
RBM10 regulates centriole duplication in HepG2 cells by ectopically assembling PLK4-STIL complexes in the nucleus. Kunimoto H, Inoue A, Kojima H, Yang J, Zhao H, Tsuruta D, Nakajima K. Genes Cells. 2020 Feb;25(2):100-110. doi: 10.1111/gtc.12741. Epub 2020 Jan 20. PubMed
Monkeys mutant for PKD1 recapitulate human autosomal dominant polycystic kidney disease. Tsukiyama T, Kobayashi K, Nakaya M, Iwatani C, Seita Y, Tsuchiya H, Matsushita J, Kitajima K, Kawamoto I, Nakagawa T, Fukuda K, Iwakiri T, Izumi H, Itagaki I, Kume S, Maegawa H, Nishinakamura R, Nishio S, Nakamura S, Kawauchi A, Ema M. Nat Commun. 2019 Dec 11;10(1):5517. doi: 10.1038/s41467-019-13398-6. PubMed
A qPCR method for genome editing efficiency determination and single-cell clone screening in human cells. Li B, Ren N, Yang L, Liu J, Huang Q. Sci Rep. 2019 Dec 11;9(1):18877. doi: 10.1038/s41598-019-55463-6. PubMed
Phosphatidylinositol 3,4-bisphosphate synthesis and turnover are spatially segregated in the endocytic pathway. Wang H, Loerke D, Bruns C, Muller R, Koch PA, Puchkov D, Schultz C, Haucke V. J Biol Chem. 2020 Jan 24;295(4):1091-1104. doi: 10.1074/jbc.RA119.011774. Epub 2019 Dec 12. PubMed
Evolution of imprinting via lineage-specific insertion of retroviral promoters. Bogutz AB, Brind'Amour J, Kobayashi H, Jensen KN, Nakabayashi K, Imai H, Lorincz MC, Lefebvre L. Nat Commun. 2019 Dec 12;10(1):5674. doi: 10.1038/s41467-019-13662-9. PubMed
Ly49R activation receptor drives self-MHC-educated NK cell immunity against cytomegalovirus infection. Gamache A, Cronk JM, Nash WT, Puchalski P, Gillespie A, Wei H, Gray L, Hammarskjold ML, Xu W, Brown MG. Proc Natl Acad Sci U S A. 2019 Dec 16. pii: 1913064117. doi: 10.1073/pnas.1913064117. PubMed
SMARCAD1-mediated recruitment of the DNA mismatch repair protein MutLalpha to MutSalpha on damaged chromatin induces apoptosis in human cells. Takeishi Y, Fujikane R, Rikitake M, Obayashi Y, Sekiguchi M, Hidaka M. J Biol Chem. 2020 Jan 24;295(4):1056-1065. doi: 10.1074/jbc.RA119.008854. Epub 2019 Dec 16. PubMed
Ablation of Bscl2/seipin in hepatocytes does not cause metabolic dysfunction in congenital generalised lipodystrophy. Mcilroy GD, Mitchell SE, Han W, Delibegovic M, Rochford JJ. Dis Model Mech. 2020 Jan 17;13(1). pii: dmm.042655. doi: 10.1242/dmm.042655. PubMed
Cas12a mediates efficient and precise endogenous gene tagging via MITI: microhomology-dependent targeted integrations. Li P, Zhang L, Li Z, Xu C, Du X, Wu S. Cell Mol Life Sci. 2019 Dec 17. pii: 10.1007/s00018-019-03396-8. doi: 10.1007/s00018-019-03396-8. PubMed
Identification of alpha,beta-Hydrolase Domain Containing Protein 6 as a Diacylglycerol Lipase in Neuro-2a Cells. van Esbroeck ACM, Kantae V, Di X, van der Wel T, den Dulk H, Stevens AF, Singh S, Bakker AT, Florea BI, Stella N, Overkleeft HS, Hankemeier T, van der Stelt M. Front Mol Neurosci. 2019 Nov 26;12:286. doi: 10.3389/fnmol.2019.00286. eCollection 2019. PubMed
MyoD induced enhancer RNA interacts with hnRNPL to activate target gene transcription during myogenic differentiation. Zhao Y, Zhou J, He L, Li Y, Yuan J, Sun K, Chen X, Bao X, Esteban MA, Sun H, Wang H. Nat Commun. 2019 Dec 19;10(1):5787. doi: 10.1038/s41467-019-13598-0. PubMed
Generation of pRSAD2 gene knock-in pig via CRISPR/Cas9 technology. Xie Z, Jiao H, Xiao H, Jiang Y, Liu Z, Qi C, Zhao D, Jiao S, Yu T, Tang X, Pang D, Ouyang H. Antiviral Res. 2020 Feb;174:104696. doi: 10.1016/j.antiviral.2019.104696. Epub 2019 Dec 17. PubMed
Target Discovery of Selective Non-Small-Cell Lung Cancer Toxins Reveals Inhibitors of Mitochondrial Complex I. Madhusudhan N, Hu B, Mishra P, Calva-Moreno JF, Patel K, Boriack R, Ready JM, Nijhawan D. ACS Chem Biol. 2020 Jan 17;15(1):158-170. doi: 10.1021/acschembio.9b00734. Epub 2019 Dec 24. PubMed
Intra-Vkappa Cluster Recombination Shapes the Ig Kappa Locus Repertoire. Shinoda K, Maman Y, Canela A, Schatz DG, Livak F, Nussenzweig A. Cell Rep. 2019 Dec 24;29(13):4471-4481.e6. doi: 10.1016/j.celrep.2019.11.088. PubMed
Synapse alterations precede neuronal damage and storage pathology in a human cerebral organoid model of CLN3-juvenile neuronal ceroid lipofuscinosis. Gomez-Giro G, Arias-Fuenzalida J, Jarazo J, Zeuschner D, Ali M, Possemis N, Bolognin S, Halder R, Jager C, Kuper WFE, van Hasselt PM, Zaehres H, Del Sol A, van der Putten H, Scholer HR, Schwamborn JC. Acta Neuropathol Commun. 2019 Dec 30;7(1):222. doi: 10.1186/s40478-019-0871-7. PubMed
Establishment of SLC15A1/PEPT1-Knockout Human-Induced Pluripotent Stem Cell Line for Intestinal Drug Absorption Studies. Kawai K, Negoro R, Ichikawa M, Yamashita T, Deguchi S, Harada K, Hirata K, Takayama K, Mizuguchi H. Mol Ther Methods Clin Dev. 2019 Nov 21;17:49-57. doi: 10.1016/j.omtm.2019.11.008. eCollection 2020 Jun 12. PubMed
Efficient Generation of Large-Fragment Knock-In Mouse Models Using 2-Cell (2C)-Homologous Recombination (HR)-CRISPR. Gu B, Posfai E, Gertsenstein M, Rossant J. Curr Protoc Mouse Biol. 2020 Mar;10(1):e67. doi: 10.1002/cpmo.67. PubMed
Hemidesmosomes modulate force generation via focal adhesions. Wang W, Zuidema A, Te Molder L, Nahidiazar L, Hoekman L, Schmidt T, Coppola S, Sonnenberg A. J Cell Biol. 2020 Feb 3;219(2). pii: 133567. doi: 10.1083/jcb.201904137. PubMed
Tesmin, Metallothionein-Like 5, is Required for Spermatogenesis in Micedagger. Oji A, Isotani A, Fujihara Y, Castaneda JM, Oura S, Ikawa M. Biol Reprod. 2020 Apr 15;102(4):975-983. doi: 10.1093/biolre/ioaa002. PubMed
Generation of Rat Monoclonal Antibodies Specific for Human Stromal Cell-Derived Factor-2. Tanaka M, Shiota M, Koyama M, Nakayama J, Yashiro M, Semba K, Goda N. Monoclon Antib Immunodiagn Immunother. 2020 Feb;39(1):23-26. doi: 10.1089/mab.2019.0043. Epub 2020 Jan 9. PubMed
The Complete Genome of Emcibacter congregatus ZYL(T), a Marine Bacterium Encoding a CRISPR-Cas 9 Immune System. Zhao Z, Zhang RA, Fu GY, Zhang R, Nie YF, Sun C, Wu M. Curr Microbiol. 2020 May;77(5):762-768. doi: 10.1007/s00284-019-01867-6. Epub 2020 Jan 9. PubMed
Beyond the heterodimer model for mineralocorticoid and glucocorticoid receptor interactions in nuclei and at DNA. Pooley JR, Rivers CA, Kilcooley MT, Paul SN, Cavga AD, Kershaw YM, Muratcioglu S, Gursoy A, Keskin O, Lightman SL. PLoS One. 2020 Jan 10;15(1):e0227520. doi: 10.1371/journal.pone.0227520. eCollection 2020. PubMed
Recapitulation of gametic DNA methylation and its post-fertilization maintenance with reassembled DNA elements at the mouse Igf2/H19 locus. Matsuzaki H, Kuramochi D, Okamura E, Hirakawa K, Ushiki A, Tanimoto K. Epigenetics Chromatin. 2020 Jan 14;13(1):2. doi: 10.1186/s13072-019-0326-1. PubMed
Generation of IL17RB Knockout Cell Lines Using CRISPR/Cas9-Based Genome Editing. Hu O, Provvido A, Zhu Y. Methods Mol Biol. 2020;2108:345-353. doi: 10.1007/978-1-0716-0247-8_28. PubMed
Upregulation of BCAM and its sense lncRNA BAN are associated with gastric cancer metastasis and poor prognosis. Jin J, Xie S, Sun Q, Huang Z, Chen K, Guo D, Rao X, Deng Y, Liu Y, Li S, Cui W, Maibam VC, Wang J, Zhuo W, Zhou T. Mol Oncol. 2020 Apr;14(4):829-845. doi: 10.1002/1878-0261.12638. Epub 2020 Feb 13. PubMed
A Universal Surrogate Reporter for Efficient Enrichment of CRISPR/Cas9-Mediated Homology-Directed Repair in Mammalian Cells. Yan N, Sun Y, Fang Y, Deng J, Mu L, Xu K, Mymryk JS, Zhang Z. Mol Ther Nucleic Acids. 2020 Mar 6;19:775-789. doi: 10.1016/j.omtn.2019.12.021. Epub 2019 Dec 24. PubMed Associated Plasmids pHDR-USR-SpCas9 pHDR-USR-eGFP
Fabrication and characterization of PLGA nanoparticles encapsulating large CRISPR-Cas9 plasmid. Jo A, Ringel-Scaia VM, McDaniel DK, Thomas CA, Zhang R, Riffle JS, Allen IC, Davis RM. J Nanobiotechnology. 2020 Jan 20;18(1):16. doi: 10.1186/s12951-019-0564-1. PubMed
Epitope Tagging ChIP-Seq of DNA Binding Proteins Using CETCh-Seq. Meadows SK, Brandsmeier LA, Newberry KM, Betti MJ, Nesmith AS, Mackiewicz M, Partridge EC, Mendenhall EM, Myers RM. Methods Mol Biol. 2020;2117:3-34. doi: 10.1007/978-1-0716-0301-7_1. PubMed
CRISPR/Cas9-Mediated TERT Disruption in Cancer Cells. Wen L, Zhao C, Song J, Ma L, Ruan J, Xia X, Chen YE, Zhang J, Ma PX, Xu J. Int J Mol Sci. 2020 Jan 19;21(2). pii: ijms21020653. doi: 10.3390/ijms21020653. PubMed
Structural insight into small molecule action on Frizzleds. Kozielewicz P, Turku A, Bowin CF, Petersen J, Valnohova J, Canizal MCA, Ono Y, Inoue A, Hoffmann C, Schulte G. Nat Commun. 2020 Jan 21;11(1):414. doi: 10.1038/s41467-019-14149-3. PubMed
Site-directed targeting of transcriptional activation-associated proteins to repressed chromatin restores CRISPR activity. Daer R, Hamna F, Barrett CM, Haynes KA. APL Bioeng. 2020 Jan 14;4(1):016102. doi: 10.1063/1.5127302. eCollection 2020 Mar. PubMed
CRISPR/Cas9-mediated knockin of human factor IX into swine factor IX locus effectively alleviates bleeding in hemophilia B pigs. Chen J, An B, Yu B, Peng X, Yuan H, Yang Q, Chen X, Yu T, Wang L, Zhang X, Wang H, Zou X, Pang D, Ouyang H, Tang X. Haematologica. 2020 Jan 23. pii: haematol.2019.224063. doi: 10.3324/haematol.2019.224063. PubMed
Murine Surf4 is essential for early embryonic development. Emmer BT, Lascuna PJ, Tang VT, Kotnik EN, Saunders TL, Khoriaty R, Ginsburg D. PLoS One. 2020 Jan 24;15(1):e0227450. doi: 10.1371/journal.pone.0227450. eCollection 2020. PubMed
Generation of a DAPK1 knockout first (conditional ready) human embryonic stem cell line (ZSSYe001-A) by CRISPR-Cas9 technology. Xu C, Zhou Z, Liu C, Kang X, Zhong X, Zhang Q, Xu Y. Stem Cell Res. 2020 Mar;43:101693. doi: 10.1016/j.scr.2019.101693. Epub 2019 Dec 21. PubMed
Phosphatidic acid regulates subcellular distribution of RA-GEFs critical for chemokine-dependent migration. Momoi Y, Nishikimi A, Du G, Kataoka T, Katagiri K. Biochem Biophys Res Commun. 2020 Apr 2;524(2):325-331. doi: 10.1016/j.bbrc.2020.01.080. Epub 2020 Jan 26. PubMed
Blocking of the CXCR4-CXCL12 Interaction Inhibits the Migration of Chicken B Cells Into the Bursa of Fabricius. Laparidou M, Schlickenrieder A, Thoma T, Lengyel K, Schusser B. Front Immunol. 2020 Jan 10;10:3057. doi: 10.3389/fimmu.2019.03057. eCollection 2019. PubMed
Gemcitabine induces Parkin-independent mitophagy through mitochondrial-resident E3 ligase MUL1-mediated stabilization of PINK1. Igarashi R, Yamashita SI, Yamashita T, Inoue K, Fukuda T, Fukuchi T, Kanki T. Sci Rep. 2020 Jan 30;10(1):1465. doi: 10.1038/s41598-020-58315-w. PubMed
Oct motif variants in Beckwith-Wiedemann syndrome patients disrupt maintenance of the hypomethylated state of the H19/IGF2 imprinting control region. Kubo S, Murata C, Okamura H, Sakasegawa T, Sakurai C, Hatsuzawa K, Hori N. FEBS Lett. 2020 May;594(10):1517-1531. doi: 10.1002/1873-3468.13750. Epub 2020 Feb 17. PubMed
BLM has Contrary Effects on Repeat-Mediated Deletions, based on the Distance of DNA DSBs to a Repeat and Repeat Divergence. Mendez-Dorantes C, Tsai LJ, Jahanshir E, Lopezcolorado FW, Stark JM. Cell Rep. 2020 Feb 4;30(5):1342-1357.e4. doi: 10.1016/j.celrep.2020.01.001. PubMed
Partitioning of MLX-Family Transcription Factors to Lipid Droplets Regulates Metabolic Gene Expression. Mejhert N, Kuruvilla L, Gabriel KR, Elliott SD, Guie MA, Wang H, Lai ZW, Lane EA, Christiano R, Danial NN, Farese RV Jr, Walther TC. Mol Cell. 2020 Mar 19;77(6):1251-1264.e9. doi: 10.1016/j.molcel.2020.01.014. Epub 2020 Feb 4. PubMed
Tolloid-Like 1 Negatively Regulates Hepatic Differentiation of Human Induced Pluripotent Stem Cells Through Transforming Growth Factor Beta Signaling. Kiso A, Toba Y, Tsutsumi S, Deguchi S, Igai K, Koshino S, Tanaka Y, Takayama K, Mizuguchi H. Hepatol Commun. 2020 Jan 2;4(2):255-267. doi: 10.1002/hep4.1466. eCollection 2020 Feb. PubMed
Tumor-derived soluble CD155 inhibits DNAM-1-mediated antitumor activity of natural killer cells. Okumura G, Iguchi-Manaka A, Murata R, Yamashita-Kanemaru Y, Shibuya A, Shibuya K. J Exp Med. 2020 Mar 2;217(4). pii: 133708. doi: 10.1084/jem.20191290. PubMed
In vivo glucoregulation and tissue-specific glucose uptake in female Akt substrate 160 kDa knockout rats. Zheng X, Arias EB, Qi NR, Saunders TL, Cartee GD. PLoS One. 2020 Feb 13;15(2):e0223340. doi: 10.1371/journal.pone.0223340. eCollection 2020. PubMed
CRISPR/Cas9 engineering of albino cystinuria Type A mice. Beckermann TM, Welch RC, Williams FM, Mortlock DP, Sha F, Ikizler TA, Woodard LE, Wilson MH. Genesis. 2020 May;58(5):e23357. doi: 10.1002/dvg.23357. Epub 2020 Feb 20. PubMed
Local rewiring of genome-nuclear lamina interactions by transcription. Brueckner L, Zhao PA, van Schaik T, Leemans C, Sima J, Peric-Hupkes D, Gilbert DM, van Steensel B. EMBO J. 2020 Mar 16;39(6):e103159. doi: 10.15252/embj.2019103159. Epub 2020 Feb 21. PubMed
Cdk5 knocking out mediated by CRISPR-Cas9 genome editing for PD-L1 attenuation and enhanced antitumor immunity. Deng H, Tan S, Gao X, Zou C, Xu C, Tu K, Song Q, Fan F, Huang W, Zhang Z. Acta Pharm Sin B. 2020 Feb;10(2):358-373. doi: 10.1016/j.apsb.2019.07.004. Epub 2019 Jul 23. PubMed
Ferroptosis driven by radical oxidation of n-6 polyunsaturated fatty acids mediates acetaminophen-induced acute liver failure. Yamada N, Karasawa T, Kimura H, Watanabe S, Komada T, Kamata R, Sampilvanjil A, Ito J, Nakagawa K, Kuwata H, Hara S, Mizuta K, Sakuma Y, Sata N, Takahashi M. Cell Death Dis. 2020 Feb 24;11(2):144. doi: 10.1038/s41419-020-2334-2. PubMed
Haploid genetic screens identify SPRING/C12ORF49 as a determinant of SREBP signaling and cholesterol metabolism. Loregger A, Raaben M, Nieuwenhuis J, Tan JME, Jae LT, van den Hengel LG, Hendrix S, van den Berg M, Scheij S, Song JY, Huijbers IJ, Kroese LJ, Ottenhoff R, van Weeghel M, van de Sluis B, Brummelkamp T, Zelcer N. Nat Commun. 2020 Feb 28;11(1):1128. doi: 10.1038/s41467-020-14811-1. PubMed
Novel Engraftment and T Cell Differentiation of Human Hematopoietic Cells in ART (-/-) IL2RG (-/Y) SCID Pigs. Boettcher AN, Li Y, Ahrens AP, Kiupel M, Byrne KA, Loving CL, Cino-Ozuna AG, Wiarda JE, Adur M, Schultz B, Swanson JJ, Snella EM, Ho CS, Charley SE, Kiefer ZE, Cunnick JE, Putz EJ, Dell'Anna G, Jens J, Sathe S, Goldman F, Westin ER, Dekkers JCM, Ross JW, Tuggle CK. Front Immunol. 2020 Feb 6;11:100. doi: 10.3389/fimmu.2020.00100. eCollection 2020. PubMed
NRF3-POMP-20S Proteasome Assembly Axis Promotes Cancer Development via Ubiquitin-Independent Proteolysis of p53 and Retinoblastoma Protein. Waku T, Nakamura N, Koji M, Watanabe H, Katoh H, Tatsumi C, Tamura N, Hatanaka A, Hirose S, Katayama H, Tani M, Kubo Y, Hamazaki J, Hamakubo T, Watanabe A, Murata S, Kobayashi A. Mol Cell Biol. 2020 Apr 28;40(10). pii: MCB.00597-19. doi: 10.1128/MCB.00597-19. Print 2020 Apr 28. PubMed
ChromID identifies the protein interactome at chromatin marks. Villasenor R, Pfaendler R, Ambrosi C, Butz S, Giuliani S, Bryan E, Sheahan TW, Gable AL, Schmolka N, Manzo M, Wirz J, Feller C, von Mering C, Aebersold R, Voigt P, Baubec T. Nat Biotechnol. 2020 Mar 2. pii: 10.1038/s41587-020-0434-2. doi: 10.1038/s41587-020-0434-2. PubMed Associated Plasmids pv6_EGFP pv6_CBX1_2xChromo pv6_CBX1_2xChromo-W42A pv7_CBX1_2xChromo_noEGFP pv6_CBX7_2xChromo More ...
Pathogenic DDX3X Mutations Impair RNA Metabolism and Neurogenesis during Fetal Cortical Development. Lennox AL, Hoye ML, Jiang R, Johnson-Kerner BL, Suit LA, Venkataramanan S, Sheehan CJ, Alsina FC, Fregeau B, Aldinger KA, Moey C, Lobach I, Afenjar A, Babovic-Vuksanovic D, Bezieau S, Blackburn PR, Bunt J, Burglen L, Campeau PM, Charles P, Chung BHY, Cogne B, Curry C, D'Agostino MD, Di Donato N, Faivre L, Heron D, Innes AM, Isidor B, Keren B, Kimball A, Klee EW, Kuentz P, Kury S, Martin-Coignard D, Mirzaa G, Mignot C, Miyake N, Matsumoto N, Fujita A, Nava C, Nizon M, Rodriguez D, Blok LS, Thauvin-Robinet C, Thevenon J, Vincent M, Ziegler A, Dobyns W, Richards LJ, Barkovich AJ, Floor SN, Silver DL, Sherr EH. Neuron. 2020 May 6;106(3):404-420.e8. doi: 10.1016/j.neuron.2020.01.042. Epub 2020 Mar 4. PubMed
Precision-engineered reporter cell lines reveal ABCG2 regulation in live lung cancer cells. Kovacsics D, Brozik A, Tihanyi B, Matula Z, Borsy A, Meszaros N, Szabo E, Nemeth E, Fothi A, Zambo B, Szuts D, Varady G, Orban TI, Apati A, Sarkadi B. Biochem Pharmacol. 2020 May;175:113865. doi: 10.1016/j.bcp.2020.113865. Epub 2020 Mar 4. PubMed
Blackjack mutations improve the on-target activities of increased fidelity variants of SpCas9 with 5'G-extended sgRNAs. Kulcsar PI, Talas A, Toth E, Nyeste A, Ligeti Z, Welker Z, Welker E. Nat Commun. 2020 Mar 6;11(1):1223. doi: 10.1038/s41467-020-15021-5. PubMed Associated Plasmids pX330-Flag-WT_SpCas9 (without sgRNA; with silent mutations) pX330-Flag-eSpCas9 (without sgRNA; with silent mutations) pX330-Flag-SpCas9-HF1 (without sgRNA; with silent mutations) pX330-Flag-HypaSpCas9 (without sgRNA; with silent mutations) pX330-Flag-evoSpCas9 (without sgRNA; with silent mutations) More ...
AIG1 and ADTRP are endogenous hydrolases of fatty acid esters of hydroxy fatty acids (FAHFAs) in mice. Erikci Ertunc M, Kok BP, Parsons WH, Wang JG, Tan D, Donaldson CJ, Pinto AFM, Vaughan JM, Ngo N, Lum KM, Henry CL, Coppola AR, Niphakis MJ, Cravatt BF, Saez E, Saghatelian A. J Biol Chem. 2020 May 1;295(18):5891-5905. doi: 10.1074/jbc.RA119.012145. Epub 2020 Mar 9. PubMed
Detection of Marker-Free Precision Genome Editing and Genetic Variation through the Capture of Genomic Signatures. Billon P, Nambiar TS, Hayward SB, Zafra MP, Schatoff EM, Oshima K, Dunbar A, Breinig M, Park YC, Ryu HS, Tschaharganeh DF, Levine RL, Baer R, Ferrando A, Dow LE, Ciccia A. Cell Rep. 2020 Mar 10;30(10):3280-3295.e6. doi: 10.1016/j.celrep.2020.02.068. PubMed Associated Plasmids sgRNA BRCA2 V572I sgRNA BRCA2 V2102I sgRNA BRCA2 E2772K sgRNA BRCA2 R2842C sgRNA BRCA2 S3070F More ...
Exonuclease 1 (Exo1) Participates in Mammalian Non-Homologous End Joining and Contributes to Drug Resistance in Ovarian Cancer. He D, Li T, Sheng M, Yang B. Med Sci Monit. 2020 Mar 13;26:e918751. doi: 10.12659/MSM.918751. PubMed
Saraf-dependent activation of mTORC1 regulates cardiac growth. Sanlialp A, Schumacher D, Kiper L, Varma E, Riechert E, Ho TC, Hofmann C, Kmietczyk V, Zimmermann F, Dlugosz S, Wirth A, Gorska AA, Burghaus J, Camacho Londono JE, Katus HA, Doroudgar S, Freichel M, Volkers M. J Mol Cell Cardiol. 2020 Apr;141:30-42. doi: 10.1016/j.yjmcc.2020.03.004. Epub 2020 Mar 13. PubMed
cAMP signaling primes lung endothelial cells to activate caspase-1 during Pseudomonas aeruginosa infection. Renema P, Hardy KS, Housley N, Dunbar G, Annamdevula N, Britain A, Spadafora D, Leavesley S, Rich T, Audia JP, Alvarez DF. Am J Physiol Lung Cell Mol Physiol. 2020 May 1;318(5):L1074-L1083. doi: 10.1152/ajplung.00185.2019. Epub 2020 Mar 18. PubMed
Function of Torsin AAA+ ATPases in Pseudorabies Virus Nuclear Egress. Holper JE, Klupp BG, Luxton GWG, Franzke K, Mettenleiter TC. Cells. 2020 Mar 17;9(3). pii: cells9030738. doi: 10.3390/cells9030738. PubMed
A hypermethylation strategy utilized by enhancer-bound CARM1 to promote estrogen receptor alpha-dependent transcriptional activation and breast carcinogenesis. Peng BL, Li WJ, Ding JC, He YH, Ran T, Xie BL, Wang ZR, Shen HF, Xiao RQ, Gao WW, Ye TY, Gao X, Liu W. Theranostics. 2020 Feb 10;10(8):3451-3473. doi: 10.7150/thno.39241. eCollection 2020. PubMed
Stagewise keratinocyte differentiation from human embryonic stem cells by defined signal transduction modulators. Zhong H, Ren Z, Wang X, Miao K, Ni W, Meng Y, Lu L, Wang C, Liu W, Deng CX, Xu RH, Chen G. Int J Biol Sci. 2020 Feb 21;16(8):1450-1462. doi: 10.7150/ijbs.44414. eCollection 2020. PubMed
Aldosterone-Induced Hypertension is Sex-Dependent, Mediated by T Cells and Sensitive to GPER Activation. Dinh QN, Vinh A, Kim HA, Saini N, Broughton BRS, Chrissobolis S, Diep H, Judkins CP, Drummond GR, Sobey CG. Cardiovasc Res. 2020 Mar 26. pii: 5811759. doi: 10.1093/cvr/cvaa075. PubMed
Telomere length heterogeneity in ALT cells is maintained by PML-dependent localization of the BTR complex to telomeres. Loe TK, Li JSZ, Zhang Y, Azeroglu B, Boddy MN, Denchi EL. Genes Dev. 2020 May 1;34(9-10):650-662. doi: 10.1101/gad.333963.119. Epub 2020 Mar 26. PubMed
Hypertension induces glomerulosclerosis in phospholipase C-epsilon1 deficiency. Atchison DK, O'Connor CL, Menon R, Otto EA, Ganesh SK, Wiggins RC, Smrcka AV, Bitzer M. Am J Physiol Renal Physiol. 2020 May 1;318(5):F1177-F1187. doi: 10.1152/ajprenal.00541.2019. Epub 2020 Mar 30. PubMed
Application of Recombination -Induced Tag Exchange (RITE) to study histone dynamics in human cells. Molenaar TM, Pages-Gallego M, Meyn V, van Leeuwen F. Epigenetics. 2020 Sep;15(9):901-913. doi: 10.1080/15592294.2020.1741777. Epub 2020 Mar 31. PubMed
Efficient and risk-reduced genome editing using double nicks enhanced by bacterial recombination factors in multiple species. He X, Chen W, Liu Z, Yu G, Chen Y, Cai YJ, Sun L, Xu W, Zhong L, Gao C, Chen J, Zhang M, Yang S, Yao Y, Zhang Z, Ma F, Zhang CC, Lu HP, Yu B, Cheng TL, Qiu J, Sheng Q, Zhou HM, Lv ZR, Yan J, Zhou Y, Qiu Z, Cui Z, Zhang X, Meng A, Sun Q, Yang Y. Nucleic Acids Res. 2020 Jun 4;48(10):e57. doi: 10.1093/nar/gkaa195. PubMed
Hepatic stellate cell-specific knockout of transcriptional intermediary factor 1gamma aggravates liver fibrosis. Lee EJ, Hwang I, Lee JY, Park JN, Kim KC, Kim I, Moon D, Park H, Lee SY, Kim HS, Jun DW, Park SH, Kim HS. J Exp Med. 2020 Jun 1;217(6). pii: 151632. doi: 10.1084/jem.20190402. PubMed
A complex regulatory landscape involved in the development of mammalian external genitals. Amandio AR, Lopez-Delisle L, Bolt CC, Mascrez B, Duboule D. Elife. 2020 Apr 17;9. pii: 52962. doi: 10.7554/eLife.52962. PubMed
Establishment of MDR1-knockout human induced pluripotent stem cell line. Negoro R, Kawai K, Ichikawa M, Deguchi S, Takayama K, Mizuguchi H. Drug Metab Pharmacokinet. 2020 Jun;35(3):288-296. doi: 10.1016/j.dmpk.2020.01.009. Epub 2020 Feb 10. PubMed
Selective targeting of the oncogenic KRAS G12S mutant allele by CRISPR/Cas9 induces efficient tumor regression. Gao Q, Ouyang W, Kang B, Han X, Xiong Y, Ding R, Li Y, Wang F, Huang L, Chen L, Wang D, Dong X, Zhang Z, Li Y, Ze B, Hou Y, Yang H, Ma Y, Gu Y, Chao CC. Theranostics. 2020 Apr 6;10(11):5137-5153. doi: 10.7150/thno.42325. eCollection 2020. PubMed
CRISPR/Cas9-Mediated Foxp1 Silencing Restores Immune Surveillance in an Immunocompetent A20 Lymphoma Model. Felce SL, Anderson AP, Maguire S, Gascoyne DM, Armstrong RN, Wong KK, Li D, Banham AH. Front Oncol. 2020 Apr 3;10:448. doi: 10.3389/fonc.2020.00448. eCollection 2020. PubMed
Efficient production of large deletion and gene fragment knock-in mice mediated by genome editing with Cas9-mouse Cdt1 in mouse zygotes. Mizuno-Iijima S, Ayabe S, Kato K, Matoba S, Ikeda Y, Dinh TTH, Le HT, Suzuki H, Nakashima K, Hasegawa Y, Hamada Y, Tanimoto Y, Daitoku Y, Iki N, Ishida M, Ibrahim EAE, Nakashiba T, Hamada M, Murata K, Miwa Y, Okada-Iwabu M, Iwabu M, Yagami KI, Ogura A, Obata Y, Takahashi S, Mizuno S, Yoshiki A, Sugiyama F. Methods. 2020 Apr 22. pii: S1046-2023(19)30328-7. doi: 10.1016/j.ymeth.2020.04.007. PubMed
miR-103/107 regulates left-right asymmetry in zebrafish by modulating Kupffer's vesicle development and ciliogenesis. Heigwer J, Kutzner J, Haeussler M, Burkhalter MD, Draebing T, Juergensen L, Katus HA, Philipp M, Westhoff JH, Hassel D. Biochem Biophys Res Commun. 2020 Jun 25;527(2):432-439. doi: 10.1016/j.bbrc.2020.04.066. Epub 2020 Apr 22. PubMed
A genome-wide enrichment screen identifies NUMA1-loss as a resistance mechanism against mitotic cell-death induced by BMI1 inhibition. Gisler S, Maia ARR, Chandrasekaran G, Kopparam J, van Lohuizen M. PLoS One. 2020 Apr 28;15(4):e0227592. doi: 10.1371/journal.pone.0227592. eCollection 2020. PubMed
Regulation of eIF2alpha by RNF4 Promotes Melanoma Tumorigenesis and Therapy Resistance. Avitan-Hersh E, Feng Y, Oknin Vaisman A, Abu Ahmad Y, Zohar Y, Zhang T, Lee JS, Lazar I, Sheikh Khalil S, Feiler Y, Kluger H, Kahana C, Brown K, Ruppin E, Ronai ZA, Orian A. J Invest Dermatol. 2020 Apr 29. pii: S0022-202X(20)31404-4. doi: 10.1016/j.jid.2020.04.008. PubMed
Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome. Miyamoto T, Hosoba K, Itabashi T, Iwane AH, Akutsu SN, Ochiai H, Saito Y, Yamamoto T, Matsuura S. EMBO J. 2020 Jun 17;39(12):e103499. doi: 10.15252/embj.2019103499. Epub 2020 May 5. PubMed
Pitfalls in Single Clone CRISPR-Cas9 Mutagenesis to Fine-map Regulatory Intervals. Tian R, Pan Y, Etheridge THA, Deshmukh H, Gulick D, Gibson G, Bao G, Lee CM. Genes (Basel). 2020 May 4;11(5). pii: genes11050504. doi: 10.3390/genes11050504. PubMed
Somatic Tissue Engineering in Mouse Models Reveals an Actionable Role for WNT Pathway Alterations in Prostate Cancer Metastasis. Leibold J, Ruscetti M, Cao Z, Ho YJ, Baslan T, Zou M, Abida W, Feucht J, Han T, Barriga FM, Tsanov KM, Zamechek L, Kulick A, Amor C, Tian S, Rybczyk K, Salgado NR, Sanchez-Rivera FJ, Watson PA, de Stanchina E, Wilkinson JE, Dow LE, Abate-Shen C, Sawyers CL, Lowe SW. Cancer Discov. 2020 Jul;10(7):1038-1057. doi: 10.1158/2159-8290.CD-19-1242. Epub 2020 May 6. PubMed
Functional characterization of the human tRNA methyltransferases TRMT10A and TRMT10B. Vilardo E, Amman F, Toth U, Kotter A, Helm M, Rossmanith W. Nucleic Acids Res. 2020 Jun 19;48(11):6157-6169. doi: 10.1093/nar/gkaa353. PubMed
Efficient genome editing by CRISPR-Mb3Cas12a in mice. Wang Z, Wang Y, Wang S, Gorzalski AJ, McSwiggin H, Yu T, Castaneda-Garcia K, Prince B, Wang H, Zheng H, Yan W. J Cell Sci. 2020 May 11;133(9). pii: 133/9/jcs240705. doi: 10.1242/jcs.240705. PubMed
A novel CDK-independent function of p27(Kip1) in preciliary vesicle trafficking during ciliogenesis. Yukimoto H, Miyamoto T, Kiyono T, Wang S, Matsuura S, Mizoguchi A, Katayama N, Inagaki M, Kasahara K. Biochem Biophys Res Commun. 2020 Jun 30;527(3):716-722. doi: 10.1016/j.bbrc.2020.05.048. Epub 2020 May 16. PubMed
RNF8 has both KU-dependent and independent roles in chromosomal break repair. Tsai LJ, Lopezcolorado FW, Bhargava R, Mendez-Dorantes C, Jahanshir E, Stark JM. Nucleic Acids Res. 2020 Jun 19;48(11):6032-6052. doi: 10.1093/nar/gkaa380. PubMed
CRISPR/dCas9-based Scn1a gene activation in inhibitory neurons ameliorates epileptic and behavioral phenotypes of Dravet syndrome model mice. Yamagata T, Raveau M, Kobayashi K, Miyamoto H, Tatsukawa T, Ogiwara I, Itohara S, Hensch TK, Yamakawa K. Neurobiol Dis. 2020 Jul;141:104954. doi: 10.1016/j.nbd.2020.104954. Epub 2020 May 21. PubMed
A CRISPR-engineered swine model of COL2A1 deficiency recapitulates altered early skeletal developmental defects in humans. Zhang B, Wang C, Zhang Y, Jiang Y, Qin Y, Pang D, Zhang G, Liu H, Xie Z, Yuan H, Ouyang H, Wang J, Tang X. Bone. 2020 Aug;137:115450. doi: 10.1016/j.bone.2020.115450. Epub 2020 May 22. PubMed
DNA replication stress induced by trifluridine determines tumor cell fate according to p53 status. Kataoka Y, Iimori M, Fujisawa R, Morikawa-Ichinose T, Niimi S, Wakasa T, Saeki H, Oki E, Miura D, Tsurimoto T, Maehara Y, Kitao H. Mol Cancer Res. 2020 May 28. pii: 1541-7786.MCR-19-1051. doi: 10.1158/1541-7786.MCR-19-1051. PubMed
Development of a Proline-Based Selection System for Reliable Genetic Engineering in Chinese Hamster Ovary Cells. Sun T, Kwok WC, Chua KJ, Lo TM, Potter J, Yew WS, Chesnut JD, Hwang IY, Chang MW. ACS Synth Biol. 2020 Jul 17;9(7):1864-1872. doi: 10.1021/acssynbio.0c00221. Epub 2020 Jun 12. PubMed
Generation of an antibody recognizing a set of acetylated proteins, including subunits of BAF complexes. Takagi M. Biochem Biophys Rep. 2019 Dec 14;22:100720. doi: 10.1016/j.bbrep.2019.100720. eCollection 2020 Jul. PubMed
5' modifications to CRISPR-Cas9 gRNA can change the dynamics and size of R-loops and inhibit DNA cleavage. Mullally G, van Aelst K, Naqvi MM, Diffin FM, Karvelis T, Gasiunas G, Siksnys V, Szczelkun MD. Nucleic Acids Res. 2020 Jul 9;48(12):6811-6823. doi: 10.1093/nar/gkaa477. PubMed
High-Resolution In Vivo Identification of miRNA Targets by Halo-Enhanced Ago2 Pull-Down. Li X, Pritykin Y, Concepcion CP, Lu Y, La Rocca G, Zhang M, King B, Cook PJ, Au YW, Popow O, Paulo JA, Otis HG, Mastroleo C, Ogrodowski P, Schreiner R, Haigis KM, Betel D, Leslie CS, Ventura A. Mol Cell. 2020 Jul 2;79(1):167-179.e11. doi: 10.1016/j.molcel.2020.05.009. Epub 2020 Jun 3. PubMed
REV7 is required for processing AID initiated DNA lesions in activated B cells. Yang D, Sun Y, Chen J, Zhang Y, Fan S, Huang M, Xie X, Cai Y, Shang Y, Gui T, Sun L, Hu J, Dong J, Yeap LS, Wang X, Xiao W, Meng FL. Nat Commun. 2020 Jun 4;11(1):2812. doi: 10.1038/s41467-020-16632-8. PubMed
Multidimensional imaging of liver injury repair in mice reveals fundamental role of the ductular reaction. Kamimoto K, Nakano Y, Kaneko K, Miyajima A, Itoh T. Commun Biol. 2020 Jun 5;3(1):289. doi: 10.1038/s42003-020-1006-1. PubMed
Elimination of Calm1 long 3' UTR mRNA isoform by CRISPR-Cas9 gene editing impairs dorsal root ganglion development and hippocampal neuron activation in mice. Bae B, Gruner HN, Lynch M, Feng T, So K, Oliver D, Mastick GS, Yan W, Pieraut S, Miura P. RNA. 2020 Jun 10. pii: rna.076430.120. doi: 10.1261/rna.076430.120. PubMed
Inhibition of HMGB1 Overcomes Resistance to Radiation and Chemotherapy in Nasopharyngeal Carcinoma. Zhu X, Cong J, Lin Z, Sun J, Yang B, Li A. Onco Targets Ther. 2020 May 14;13:4189-4199. doi: 10.2147/OTT.S239243. eCollection 2020. PubMed
Defining Transcription Regulatory Elements in the Human Frataxin Gene: Implications for Gene Therapy. Li J, Li Y, Wang J, Gonzalez TJ, Asokan A, Napierala JS, Napierala M. Hum Gene Ther. 2020 Aug;31(15-16):839-851. doi: 10.1089/hum.2020.053. Epub 2020 Jul 13. PubMed Associated Plasmids pCR4_miniFXN_7 pCR4_miniFXN_8
Enhanced NK-92 Cytotoxicity by CRISPR Genome Engineering Using Cas9 Ribonucleoproteins. Huang RS, Shih HA, Lai MC, Chang YJ, Lin S. Front Immunol. 2020 May 22;11:1008. doi: 10.3389/fimmu.2020.01008. eCollection 2020. PubMed
Tmprss12 is required for sperm motility and uterotubal junction migration in micedagger. Larasati T, Noda T, Fujihara Y, Shimada K, Tobita T, Yu Z, Matzuk MM, Ikawa M. Biol Reprod. 2020 Aug 4;103(2):254-263. doi: 10.1093/biolre/ioaa060. PubMed
A recurrent PJA1 variant in trigonocephaly and neurodevelopmental disorders. Suzuki T, Suzuki T, Raveau M, Miyake N, Sudo G, Tsurusaki Y, Watanabe T, Sugaya Y, Tatsukawa T, Mazaki E, Shimohata A, Kushima I, Aleksic B, Shiino T, Toyota T, Iwayama Y, Nakaoka K, Ohmori I, Sasaki A, Watanabe K, Hirose S, Kaneko S, Inoue Y, Yoshikawa T, Ozaki N, Kano M, Shimoji T, Matsumoto N, Yamakawa K. Ann Clin Transl Neurol. 2020 Jul;7(7):1117-1131. doi: 10.1002/acn3.51093. Epub 2020 Jun 12. PubMed
Increasing CRISPR/Cas9-mediated homology-directed DNA repair by histone deacetylase inhibitors. Li G, Zhang X, Wang H, Liu D, Li Z, Wu Z, Yang H. Int J Biochem Cell Biol. 2020 Aug;125:105790. doi: 10.1016/j.biocel.2020.105790. Epub 2020 Jun 10. PubMed
CRISPR/Cas9-mediated genome-edited mice reveal 10 testis-enriched genes are dispensable for male fecundity. Park S, Shimada K, Fujihara Y, Xu Z, Shimada K, Larasati T, Pratiwi P, Matzuk RM, Devlin DJ, Yu Z, Garcia TX, Matzuk MM, Ikawa M. Biol Reprod. 2020 Aug 4;103(2):195-204. doi: 10.1093/biolre/ioaa084. PubMed
MICOS assembly controls mitochondrial inner membrane remodeling and crista junction redistribution to mediate cristae formation. Stephan T, Bruser C, Deckers M, Steyer AM, Balzarotti F, Barbot M, Behr TS, Heim G, Hubner W, Ilgen P, Lange F, Pacheu-Grau D, Pape JK, Stoldt S, Huser T, Hell SW, Mobius W, Rehling P, Riedel D, Jakobs S. EMBO J. 2020 Jul 15;39(14):e104105. doi: 10.15252/embj.2019104105. Epub 2020 Jun 22. PubMed
Studies on CRMP2 SUMOylation-deficient transgenic mice identify sex-specific NaV1.7 regulation in the pathogenesis of chronic neuropathic pain. Moutal A, Cai S, Yu J, Stratton HJ, Chefdeville A, Gomez K, Ran D, Madura CL, Boinon L, Soto M, Zhou Y, Shan Z, Chew LA, Rodgers KE, Khanna R. Pain. 2020 Jun 11. doi: 10.1097/j.pain.0000000000001951. PubMed
Novel gene Merlot inhibits differentiation and promotes apoptosis of osteoclasts. Yamakawa T, Okamatsu N, Ishikawa K, Kiyohara S, Handa K, Hayashi E, Sakai N, Karakawa A, Chatani M, Tsuji M, Inagaki K, Kiuchi Y, Negishi-Koga T, Takami M. Bone. 2020 Sep;138:115494. doi: 10.1016/j.bone.2020.115494. Epub 2020 Jun 20. PubMed
Comparative targeting analysis of KLF1, BCL11A, and HBG1/2 in CD34(+) HSPCs by CRISPR/Cas9 for the induction of fetal hemoglobin. Lamsfus-Calle A, Daniel-Moreno A, Antony JS, Epting T, Heumos L, Baskaran P, Admard J, Casadei N, Latifi N, Siegmund DM, Kormann MSD, Handgretinger R, Mezger M. Sci Rep. 2020 Jun 23;10(1):10133. doi: 10.1038/s41598-020-66309-x. PubMed
Exoenzyme Y induces extracellular active caspase-7 accumulation independent from apoptosis: modulation of transmissible cytotoxicity. Renema P, Kozhukhar N, Pastukh V, Spadafora D, Paudel SS, Tambe DT, Alexeyev M, Frank DW, Stevens T. Am J Physiol Lung Cell Mol Physiol. 2020 Aug 1;319(2):L380-L390. doi: 10.1152/ajplung.00508.2019. Epub 2020 Jun 24. PubMed
Ppp2r2a Knockout Mice Reveal That Protein Phosphatase 2A Regulatory Subunit, PP2A-B55alpha, Is an Essential Regulator of Neuronal and Epidermal Embryonic Development. Panicker N, Coutman M, Lawlor-O'Neill C, Kahl RGS, Roselli S, Verrills NM. Front Cell Dev Biol. 2020 Jun 5;8:358. doi: 10.3389/fcell.2020.00358. eCollection 2020. PubMed
Tyrosyl phosphorylation of PZR promotes hypertrophic cardiomyopathy in PTPN11-associated Noonan syndrome with multiple lentigines. Yi JS, Perla S, Enyenihi L, Bennett AM. JCI Insight. 2020 Aug 6;5(15). pii: 137753. doi: 10.1172/jci.insight.137753. PubMed
Chemical genetics strategy to profile kinase target engagement reveals role of FES in neutrophil phagocytosis. van der Wel T, Hilhorst R, den Dulk H, van den Hooven T, Prins NM, Wijnakker JAPM, Florea BI, Lenselink EB, van Westen GJP, Ruijtenbeek R, Overkleeft HS, Kaptein A, Barf T, van der Stelt M. Nat Commun. 2020 Jun 25;11(1):3216. doi: 10.1038/s41467-020-17027-5. PubMed
ATAD5 suppresses centrosome over-duplication by regulating UAF1 and ID1. Kim SJ, Wie M, Park SH, Kim TM, Park JH, Kim S, Myung K, Lee KY. Cell Cycle. 2020 Aug;19(15):1952-1968. doi: 10.1080/15384101.2020.1785724. Epub 2020 Jun 28. PubMed
RanBP1 controls the Ran pathway in mammalian cells through regulation of mitotic RCC1 dynamics. Yau KC, Arnaoutov A, Aksenova V, Kaufhold R, Chen S, Dasso M. Cell Cycle. 2020 Aug;19(15):1899-1916. doi: 10.1080/15384101.2020.1782036. Epub 2020 Jun 28. PubMed
PDGFA-associated protein 1 protects mature B lymphocytes from stress-induced cell death and promotes antibody gene diversification. Delgado-Benito V, Berruezo-Llacuna M, Altwasser R, Winkler W, Sundaravinayagam D, Balasubramanian S, Caganova M, Graf R, Rahjouei A, Henke MT, Driesner M, Keller L, Prigione A, Janz M, Akalin A, Di Virgilio M. J Exp Med. 2020 Oct 5;217(10). pii: 151913. doi: 10.1084/jem.20200137. PubMed
Cell type- and stage-specific expression of Otx2 is regulated by multiple transcription factors and cis-regulatory modules in the retina. Chan CSY, Lonfat N, Zhao R, Davis AE, Li L, Wu MR, Lin CH, Ji Z, Cepko CL, Wang S. Development. 2020 Jul 26;147(14). pii: dev.187922. doi: 10.1242/dev.187922. PubMed
RNA is essential for PRC2 chromatin occupancy and function in human pluripotent stem cells. Long Y, Hwang T, Gooding AR, Goodrich KJ, Rinn JL, Cech TR. Nat Genet. 2020 Sep;52(9):931-938. doi: 10.1038/s41588-020-0662-x. Epub 2020 Jul 6. PubMed Associated Plasmids pYL214 pYL236 pYL237 pcDNA3.1_3xFlagEzh2 WT pcDNA3.1_3xFlagEzh2 RNA binding mutant
Leveraging systematic functional analysis to benchmark an in silico framework distinguishes driver from passenger MEK mutants in cancer. Hanrahan AJ, Sylvester BE, Chang MT, Elzein A, Gao J, Han W, Liu Y, Xu D, Gao SP, Gorelick AN, Jones AM, Kiliti AJ, Nissan MH, Nimura CA, Poteshman AN, Yao Z, Gao Y, Hu W, Wise HC, Gavrila EI, Shoushtari AN, Tiwari S, Viale A, Abdel-Wahab O, Merghoub T, Berger MF, Rosen N, Taylor BS, Solit DB. Cancer Res. 2020 Jul 8. pii: 0008-5472.CAN-20-0865. doi: 10.1158/0008-5472.CAN-20-0865. PubMed
Cerebral cavernous malformations are driven by ADAMTS5 proteolysis of versican. Hong CC, Tang AT, Detter MR, Choi JP, Wang R, Yang X, Guerrero AA, Wittig CF, Hobson N, Girard R, Lightle R, Moore T, Shenkar R, Polster SP, Goddard LM, Ren AA, Leu NA, Sterling S, Yang J, Li L, Chen M, Mericko-Ishizuka P, Dow LE, Watanabe H, Schwaninger M, Min W, Marchuk DA, Zheng X, Awad IA, Kahn ML. J Exp Med. 2020 Oct 5;217(10). pii: 151938. doi: 10.1084/jem.20200140. PubMed
DNMT3B deficiency alters mitochondrial biogenesis and alpha-ketoglutarate levels in human embryonic stem cells. Cieslar-Pobuda A, Ahrens TD, Caglayan S, Behringer S, Hannibal L, Staerk J. Stem Cells. 2020 Jul 11. doi: 10.1002/stem.3256. PubMed
Genetic dissection of Ragulator structure and function in amino acid-dependent regulation of mTORC1. Nada S, Okada M. J Biochem. 2020 Jul 11. pii: 5870373. doi: 10.1093/jb/mvaa076. PubMed
L1CAM defines the regenerative origin of metastasis-initiating cells in colorectal cancer. Ganesh K, Basnet H, Kaygusuz Y, Laughney AM, He L, Sharma R, O'Rourke KP, Reuter VP, Huang YH, Turkekul M, Emrah E, Masilionis I, Manova-Todorova K, Weiser MR, Saltz LB, Garcia-Aguilar J, Koche R, Lowe SW, Pe'er D, Shia J, Massague J. Nat Cancer. 2020 Jan;1(1):28-45. doi: 10.1038/s43018-019-0006-x. Epub 2020 Jan 13. PubMed
The PRDM14-CtBP1/2-PRC2 complex regulates transcriptional repression during the transition from primed to naive pluripotency. Yamamoto M, Suwa Y, Sugiyama K, Okashita N, Kawaguchi M, Tani N, Matsubara K, Nakamura A, Seki Y. J Cell Sci. 2020 Aug 14;133(15). pii: jcs.240176. doi: 10.1242/jcs.240176. PubMed
Cdc14a has a role in spermatogenesis, sperm maturation and male fertility. Wen Z, Zhu H, Zhang A, Lin J, Zhang G, Liu D, Xiao Y, Ye C, Sun D, Wu B, Zhang J, Gao J. Exp Cell Res. 2020 Jul 14:112178. doi: 10.1016/j.yexcr.2020.112178. PubMed
CTCF orchestrates long-range cohesin-driven V(D)J recombinational scanning. Ba Z, Lou J, Ye AY, Dai HQ, Dring EW, Lin SG, Jain S, Kyritsis N, Kieffer-Kwon KR, Casellas R, Alt FW. Nature. 2020 Oct;586(7828):305-310. doi: 10.1038/s41586-020-2578-0. Epub 2020 Jul 27. PubMed
Reduction of advanced tau-mediated memory deficits by the MAP kinase p38gamma. Ittner A, Asih PR, Tan ARP, Prikas E, Bertz J, Stefanoska K, Lin Y, Volkerling AM, Ke YD, Delerue F, Ittner LM. Acta Neuropathol. 2020 Sep;140(3):279-294. doi: 10.1007/s00401-020-02191-1. Epub 2020 Jul 29. PubMed
Induced Liver Regeneration Enhances CRISPR/Cas9-Mediated Gene Repair in Tyrosinemia Type 1. Zhang QS, Tiyaboonchai A, Nygaard S, Baradar K, Major A, Balaji N, Grompe M. Hum Gene Ther. 2021 Mar;32(5-6):294-301. doi: 10.1089/hum.2020.042. Epub 2020 Oct 16. PubMed
Generation of Gene-Knockout Mongolian Gerbils via CRISPR/Cas9 System. Wang Y, Zhao P, Song Z, Du X, Huo X, Lu J, Liu X, Lv J, Li C, Guo M, Chen Z. Front Bioeng Biotechnol. 2020 Jul 8;8:780. doi: 10.3389/fbioe.2020.00780. eCollection 2020. PubMed
ABHD11 Is Critical for Embryonic Stem Cell Expansion, Differentiation and Lipid Metabolic Homeostasis. Liu G, Ruan Y, Zhang J, Wang X, Wu W, He P, Wang J, Xiong J, Cheng Y, Liu L, Yang Y, Tian Y, Jian R. Front Cell Dev Biol. 2020 Jul 7;8:570. doi: 10.3389/fcell.2020.00570. eCollection 2020. PubMed
Inhibition of protein kinase D by CID755673 promotes maintenance of the pluripotency of embryonic stem cells. Zhu Z, Zhang Y, Wang X, Wang X, Ye SD. Development. 2020 Aug 24;147(16):dev185264. doi: 10.1242/dev.185264. PubMed
Direct analysis of brain phenotypes via neural blastocyst complementation. Dai HQ, Liang Z, Chang AN, Chapdelaine-Williams AM, Alvarado B, Pollen AA, Alt FW, Schwer B. Nat Protoc. 2020 Oct;15(10):3154-3181. doi: 10.1038/s41596-020-0364-y. Epub 2020 Aug 10. PubMed
Discovery of EMRE in fungi resolves the true evolutionary history of the mitochondrial calcium uniporter. Pittis AA, Goh V, Cebrian-Serrano A, Wettmarshausen J, Perocchi F, Gabaldon T. Nat Commun. 2020 Aug 12;11(1):4031. doi: 10.1038/s41467-020-17705-4. PubMed
The aryl hydrocarbon receptor promotes inflammation-induced dedifferentiation and systemic metastatic spread of melanoma cells. Mengoni M, Braun AD, Gaffal E, Tuting T. Int J Cancer. 2020 Nov 15;147(10):2902-2913. doi: 10.1002/ijc.33252. Epub 2020 Aug 31. PubMed
A multiplexed bioluminescent reporter for sensitive and non-invasive tracking of DNA double strand break repair dynamics in vitro and in vivo. Chien JC, Tabet E, Pinkham K, da Hora CC, Chang JC, Lin S, Badr CE, Lai CP. Nucleic Acids Res. 2020 Aug 14. pii: 5892751. doi: 10.1093/nar/gkaa669. PubMed Associated Plasmids pLenti-BLRR pLenti-trGluc pX330-gRNA
cAMP response element induces Per1 in vivo. Ikegami K, Nakajima M, Minami Y, Nagano M, Masubuchi S, Shigeyoshi Y. Biochem Biophys Res Commun. 2020 Oct 22;531(4):515-521. doi: 10.1016/j.bbrc.2020.07.105. Epub 2020 Aug 15. PubMed
Lamin A/C deficiency enables increased myosin-II bipolar filament ensembles that promote divergent actomyosin network anomalies through self-organization. Wiggan O, DeLuca JG, Stasevich TJ, Bamburg JR. Mol Biol Cell. 2020 Oct 1;31(21):2363-2378. doi: 10.1091/mbc.E20-01-0017-T. Epub 2020 Aug 20. PubMed
Visualization of Chromatin Dynamics by Live Cell Microscopy Using CRISPR/Cas9 Gene Editing and ANCHOR Labeling. Fok ET, Fanucchi S, Bystricky K, Mhlanga MM. Methods Mol Biol. 2021;2157:197-212. doi: 10.1007/978-1-0716-0664-3_11. PubMed
beta-Catenin safeguards the ground state of mousepluripotency by strengthening the robustness of the transcriptional apparatus. Zhang M, Lai Y, Krupalnik V, Guo P, Guo X, Zhou J, Xu Y, Yu Z, Liu L, Jiang A, Li W, Abdul MM, Ma G, Li N, Fu X, Lv Y, Jiang M, Tariq M, Kanwal S, Liu H, Xu X, Zhang H, Huang Y, Wang L, Chen S, Babarinde IA, Luo Z, Wang D, Zhou T, Ward C, He M, Ibanez DP, Li Y, Zhou J, Yuan J, Feng Y, Arumugam K, Di Vicino U, Bao X, Wu G, Schambach A, Wang H, Sun H, Gao F, Qin B, Hutchins AP, Doble BW, Hartmann C, Cosma MP, Qin Y, Xu GL, Chen R, Volpe G, Chen L, Hanna JH, Esteban MA. Sci Adv. 2020 Jul 17;6(29):eaba1593. doi: 10.1126/sciadv.aba1593. eCollection 2020 Jul. PubMed
A positive, growth-based PAM screen identifies noncanonical motifs recognized by the S. pyogenes Cas9. Collias D, Leenay RT, Slotkowski RA, Zuo Z, Collins SP, McGirr BA, Liu J, Beisel CL. Sci Adv. 2020 Jul 15;6(29):eabb4054. doi: 10.1126/sciadv.abb4054. eCollection 2020 Jul. PubMed
Low-Density Lipoprotein Receptor-Related Protein 5-Deficient Rats Have Reduced Bone Mass and Abnormal Development of the Retinal Vasculature. Ubels JL, Diegel CR, Foxa GE, Ethen NJ, Lensing JN, Madaj ZB, Williams BO. CRISPR J. 2020 Aug;3(4):284-298. doi: 10.1089/crispr.2020.0009. PubMed
Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence. Ishibashi R, Abe K, Ido N, Kitano S, Miyachi H, Toyoshima F. Sci Rep. 2020 Aug 24;10(1):14120. doi: 10.1038/s41598-020-70804-6. PubMed
Complete chemical structures of human mitochondrial tRNAs. Suzuki T, Yashiro Y, Kikuchi I, Ishigami Y, Saito H, Matsuzawa I, Okada S, Mito M, Iwasaki S, Ma D, Zhao X, Asano K, Lin H, Kirino Y, Sakaguchi Y, Suzuki T. Nat Commun. 2020 Aug 28;11(1):4269. doi: 10.1038/s41467-020-18068-6. PubMed
CD163 and pAPN double-knockout pigs are resistant to PRRSV and TGEV and exhibit decreased susceptibility to PDCoV while maintaining normal production performance. Xu K, Zhou Y, Mu Y, Liu Z, Hou S, Xiong Y, Fang L, Ge C, Wei Y, Zhang X, Xu C, Che J, Fan Z, Xiang G, Guo J, Shang H, Li H, Xiao S, Li J, Li K. Elife. 2020 Sep 2;9. pii: 57132. doi: 10.7554/eLife.57132. PubMed
PICH regulates the abundance and localization of SUMOylated proteins on mitotic chromosomes. Hassebroek VA, Park H, Pandey N, Lerbakken BT, Aksenova V, Arnaoutov A, Dasso M, Azuma Y. Mol Biol Cell. 2020 Nov 1;31(23):2537-2556. doi: 10.1091/mbc.E20-03-0180. Epub 2020 Sep 2. PubMed
The centriole protein CEP76 negatively regulates PLK1 activity in the cytoplasm for proper mitotic progression. Takeda Y, Yamazaki K, Hashimoto K, Watanabe K, Chinen T, Kitagawa D. J Cell Sci. 2020 Oct 1;133(19):jcs241281. doi: 10.1242/jcs.241281. PubMed
Repurposing of KLF5 activates a cell cycle signature during the progression from a precursor state to oesophageal adenocarcinoma. Rogerson C, Ogden S, Britton E, Ang Y, Sharrocks AD. Elife. 2020 Sep 3;9:e57189. doi: 10.7554/eLife.57189. PubMed
Nucleoporin TPR is an integral component of the TREX-2 mRNA export pathway. Aksenova V, Smith A, Lee H, Bhat P, Esnault C, Chen S, Iben J, Kaufhold R, Yau KC, Echeverria C, Fontoura B, Arnaoutov A, Dasso M. Nat Commun. 2020 Sep 11;11(1):4577. doi: 10.1038/s41467-020-18266-2. PubMed
Variation in HIV-1 Nef function within and among viral subtypes reveals genetically separable antagonism of SERINC3 and SERINC5. Jin SW, Mwimanzi FM, Mann JK, Bwana MB, Lee GQ, Brumme CJ, Hunt PW, Martin JN, Bangsberg DR, Ndung'u T, Brumme ZL, Brockman MA. PLoS Pathog. 2020 Sep 14;16(9):e1008813. doi: 10.1371/journal.ppat.1008813. eCollection 2020 Sep. PubMed
Controlling the Activity of CRISPR Transcriptional Regulators with Inducible sgRNAs. Ferry QRV, Fulga TA. Methods Mol Biol. 2021;2162:153-184. doi: 10.1007/978-1-0716-0687-2_9. PubMed
Using High-Content Screening to Generate Single-Cell Gene-Corrected Patient-Derived iPS Clones Reveals Excess Alpha-Synuclein with Familial Parkinson's Disease Point Mutation A30P. Barbuti P, Antony P, Santos B, Massart F, Cruciani G, Dording C, Arias J, Schwamborn J, Kruger R. Cells. 2020 Sep 10;9(9). pii: cells9092065. doi: 10.3390/cells9092065. PubMed
Plasmodium berghei sporozoites in nonreplicative vacuole are eliminated by a PI3P-mediated autophagy-independent pathway. Bindschedler A, Wacker R, Egli J, Eickel N, Schmuckli-Maurer J, Franke-Fayard BM, Janse CJ, Heussler VT. Cell Microbiol. 2021 Jan;23(1):e13271. doi: 10.1111/cmi.13271. Epub 2020 Oct 8. PubMed
Modeling polymorphic ventricular tachycardia at rest using patient-specific induced pluripotent stem cell-derived cardiomyocytes. Sleiman Y, Souidi M, Kumar R, Yang E, Jaffre F, Zhou T, Bernardin A, Reiken S, Cazorla O, Kajava AV, Moreau A, Pasquie JL, Marks AR, Lerman BB, Chen S, Cheung JW, Evans T, Lacampagne A, Meli AC. EBioMedicine. 2020 Oct;60:103024. doi: 10.1016/j.ebiom.2020.103024. Epub 2020 Sep 24. PubMed
BH3-only sensors Bad, Noxa and Puma are Key Regulators of Tacaribe virus-induced Apoptosis. Holzerland J, Feneant L, Banadyga L, Holper JE, Knittler MR, Groseth A. PLoS Pathog. 2020 Oct 12;16(10):e1008948. doi: 10.1371/journal.ppat.1008948. eCollection 2020 Oct. PubMed
Transcriptional suppression of ribosomal DNA with phase separation. Ide S, Imai R, Ochi H, Maeshima K. Sci Adv. 2020 Oct 14;6(42). pii: 6/42/eabb5953. doi: 10.1126/sciadv.abb5953. Print 2020 Oct. PubMed
A porcine model of phenylketonuria generated by CRISPR/Cas9 genome editing. Koppes EA, Redel BK, Johnson MA, Skvorak KJ, Ghaloul-Gonzalez L, Yates ME, Lewis DW, Gollin SM, Wu YL, Christ SE, Yerle M, Leshinski A, Spate LD, Benne JA, Murphy SL, Samuel MS, Walters EM, Hansen SA, Wells KD, Lichter-Konecki U, Wagner RA, Newsome JT, Dobrowolski SF, Vockley J, Prather RS, Nicholls RD. JCI Insight. 2020 Oct 15;5(20). pii: 141523. doi: 10.1172/jci.insight.141523. PubMed
Protocol for the Generation of Human Pluripotent Reporter Cell Lines Using CRISPR/Cas9. Zhong A, Li M, Zhou T. STAR Protoc. 2020 Sep 18;1(2). doi: 10.1016/j.xpro.2020.100052. Epub 2020 Jun 13. PubMed
Enhancement of CRISPR-Cas9 induced precise gene editing by targeting histone H2A-K15 ubiquitination. Bashir S, Dang T, Rossius J, Wolf J, Kuhn R. BMC Biotechnol. 2020 Oct 23;20(1):57. doi: 10.1186/s12896-020-00650-x. PubMed Associated Plasmids pAAVS1-TLR6 pU6Rosa-CAG-Cas9 pTLR-donor pTLR-donor-tetO pTLR-donor-UAS More ...
Inhibition of SARS-CoV-2 viral entry upon blocking N- and O-glycan elaboration. Yang Q, Hughes TA, Kelkar A, Yu X, Cheng K, Park S, Huang WC, Lovell JF, Neelamegham S. Elife. 2020 Oct 26;9. pii: 61552. doi: 10.7554/eLife.61552. PubMed Associated Plasmids ACE2 S1-Fc RBD-Fc ACE2-Fc
Sensitive ELISA-based detection method for the mitophagy marker p-S65-Ub in human cells, autopsy brain, and blood samples. Watzlawik JO, Hou X, Fricova D, Ramnarine C, Barodia SK, Gendron TF, Heckman MG, DeTure M, Siuda J, Wszolek ZK, Scherzer CR, Ross OA, Bu G, Dickson DW, Goldberg MS, Fiesel FC, Springer W. Autophagy. 2021 Sep;17(9):2613-2628. doi: 10.1080/15548627.2020.1834712. Epub 2020 Oct 28. PubMed
The RIF1-long splice variant promotes G1 phase 53BP1 nuclear bodies to protect against replication stress. Watts LP, Natsume T, Saito Y, Garzon J, Dong Q, Boteva L, Gilbert N, Kanemaki MT, Hiraga SI, Donaldson AD. Elife. 2020 Nov 3;9. pii: 58020. doi: 10.7554/eLife.58020. PubMed
Mitochondrial and Clearance Impairment in p.D620N VPS35 Patient-Derived Neurons. Hanss Z, Larsen SB, Antony P, Mencke P, Massart F, Jarazo J, Schwamborn JC, Barbuti PA, Mellick GD, Kruger R. Mov Disord. 2021 Mar;36(3):704-715. doi: 10.1002/mds.28365. Epub 2020 Nov 3. PubMed
A CRISPR-Cas9, Cre-lox, and Flp-FRT Cascade Strategy for the Precise and Efficient Integration of Exogenous DNA into Cellular Genomes. Li J, Li Y, Pawlik KM, Napierala JS, Napierala M. CRISPR J. 2020 Dec;3(6):470-486. doi: 10.1089/crispr.2020.0042. Epub 2020 Nov 3. PubMed
SUMOylation of the ubiquitin ligase IDOL decreases LDL receptor levels and is reversed by SENP1. Wang JQ, Lin ZC, Li LL, Zhang SF, Li WH, Liu W, Song BL, Luo J. J Biol Chem. 2021 Jan-Jun;296:100032. doi: 10.1074/jbc.RA120.015420. Epub 2020 Nov 23. PubMed
Nuclear peripheral chromatin-lamin B1 interaction is required for global integrity of chromatin architecture and dynamics in human cells. Chang L, Li M, Shao S, Li C, Ai S, Xue B, Hou Y, Zhang Y, Li R, Fan X, He A, Li C, Sun Y. Protein Cell. 2022 Apr;13(4):258-280. doi: 10.1007/s13238-020-00794-8. Epub 2020 Nov 5. PubMed
Zmat3 Is a Key Splicing Regulator in the p53 Tumor Suppression Program. Bieging-Rolett KT, Kaiser AM, Morgens DW, Boutelle AM, Seoane JA, Van Nostrand EL, Zhu C, Houlihan SL, Mello SS, Yee BA, McClendon J, Pierce SE, Winters IP, Wang M, Connolly AJ, Lowe SW, Curtis C, Yeo GW, Winslow MM, Bassik MC, Attardi LD. Mol Cell. 2020 Nov 5;80(3):452-469.e9. doi: 10.1016/j.molcel.2020.10.022. PubMed Associated Plasmids pcDNA3.1-3XHA-Zmat3 pcDNA3.1-3XHA-Zmat3R99Q Lenti-sgZmat3.55.9/Cre Lenti-sgZmat3.52.6/Cre Lenti-sgZmat3.51.5/Cre More ...
Cre/LoxP-HBV plasmids generating recombinant covalently closed circular DNA genome upon transfection. Kruse RL, Legras X, Barzi M. Virus Res. 2021 Jan 15;292:198224. doi: 10.1016/j.virusres.2020.198224. Epub 2020 Nov 6. PubMed
A functional variant on 20q13.33 related to glioma risk alters enhancer activity and modulates expression of multiple genes. Ali MW, Patro CPK, Zhu JJ, Dampier CH, Plummer SJ, Kuscu C, Adli M, Lau C, Lai RK, Casey G. Hum Mutat. 2021 Jan;42(1):77-88. doi: 10.1002/humu.24134. Epub 2020 Nov 22. PubMed
Glioblastoma stem cells induce quiescence in surrounding neural stem cells via Notch signaling. Lawlor K, Marques-Torrejon MA, Dharmalingham G, El-Azhar Y, Schneider MD, Pollard SM, Rodriguez TA. Genes Dev. 2020 Dec 1;34(23-24):1599-1604. doi: 10.1101/gad.336917.120. Epub 2020 Nov 12. PubMed
Suppression of HIV-1 Integration by Targeting HIV-1 Integrase for Degradation with A Chimeric Ubiquitin Ligase. Zhang Z, Yuan S, Xu S, Guo D, Chen L, Hou W, Wang M. Virol Sin. 2021 Jun;36(3):424-437. doi: 10.1007/s12250-020-00311-5. Epub 2020 Nov 13. PubMed
Inactivation of the three GGA genes in HeLa cells partially compromises lysosomal enzyme sorting. Doray B, Liu L, Lee WS, Jennings BC, Kornfeld S. FEBS Open Bio. 2021 Feb;11(2):367-374. doi: 10.1002/2211-5463.13040. Epub 2021 Jan 1. PubMed
Mast4 knockout shows the regulation of spermatogonial stem cell self-renewal via the FGF2/ERM pathway. Lee SJ, Park J, Lee DJ, Otsu K, Kim P, Mizuno S, Lee MJ, Kim HY, Harada H, Takahashi S, Kim SJ, Jung HS. Cell Death Differ. 2021 May;28(5):1441-1454. doi: 10.1038/s41418-020-00670-2. Epub 2020 Nov 20. PubMed
Mechanism of Lamellar Body Formation by Lung Surfactant Protein B. Sever N, Milicic G, Bodnar NO, Wu X, Rapoport TA. Mol Cell. 2021 Jan 7;81(1):49-66.e8. doi: 10.1016/j.molcel.2020.10.042. Epub 2020 Nov 25. PubMed
NCOA4-mediated ferritinophagy promotes ferroptosis induced by erastin, but not by RSL3 in HeLa cells. Gryzik M, Asperti M, Denardo A, Arosio P, Poli M. Biochim Biophys Acta Mol Cell Res. 2021 Feb;1868(2):118913. doi: 10.1016/j.bbamcr.2020.118913. Epub 2020 Nov 25. PubMed
MCPIP1 reduces HBV-RNA by targeting its epsilon structure. Li Y, Que L, Fukano K, Koura M, Kitamura K, Zheng X, Kato T, Aly HH, Watashi K, Tsukuda S, Aizaki H, Watanabe N, Sato Y, Suzuki T, Suzuki HI, Hosomichi K, Kurachi M, Wakae K, Muramatsu M. Sci Rep. 2020 Nov 27;10(1):20763. doi: 10.1038/s41598-020-77166-z. PubMed
Generation of human striatal organoids and cortico-striatal assembloids from human pluripotent stem cells. Miura Y, Li MY, Birey F, Ikeda K, Revah O, Thete MV, Park JY, Puno A, Lee SH, Porteus MH, Pasca SP. Nat Biotechnol. 2020 Dec;38(12):1421-1430. doi: 10.1038/s41587-020-00763-w. Epub 2020 Dec 3. PubMed
Autocrine IL-6/STAT3 signaling aids development of acquired drug resistance in Group 3 medulloblastoma. Sreenivasan L, Wang H, Yap SQ, Leclair P, Tam A, Lim CJ. Cell Death Dis. 2020 Dec 5;11(12):1035. doi: 10.1038/s41419-020-03241-y. PubMed
Transcriptional behavior of the HIV-1 promoter in context of the BACH2 prominent proviral integration gene. Hamann MV, Ehmele P, Verdikt R, Bialek-Waldmann JK, Virdi S, Gunther T, Van Lint C, Grundhoff A, Hauber J, Lange UC. Virus Res. 2021 Feb;293:198260. doi: 10.1016/j.virusres.2020.198260. Epub 2020 Dec 11. PubMed
WAPL maintains a cohesin loading cycle to preserve cell-type-specific distal gene regulation. Liu NQ, Maresca M, van den Brand T, Braccioli L, Schijns MMGA, Teunissen H, Bruneau BG, Nora EP, de Wit E. Nat Genet. 2021 Jan;53(1):100-109. doi: 10.1038/s41588-020-00744-4. Epub 2020 Dec 14. PubMed Associated Plasmids pEN527 - Rad21-AID[71-114]-eGFP-FRT-Blast-FRT targeting construct pNQL001-WAPL-AID[71-114]-eGFP-FRT-Neo/Kan-FRT targeting construct pX335-NQL002-WAPL-sgRNA1 pX335-NQL003-WAPL-sgRNA2 pNQL004-SOX2-FKBPV-HA2-P2A-mCherry targeting construct More ...
Modeling Human TBX5 Haploinsufficiency Predicts Regulatory Networks for Congenital Heart Disease. Kathiriya IS, Rao KS, Iacono G, Devine WP, Blair AP, Hota SK, Lai MH, Garay BI, Thomas R, Gong HZ, Wasson LK, Goyal P, Sukonnik T, Hu KM, Akgun GA, Bernard LD, Akerberg BN, Gu F, Li K, Speir ML, Haeussler M, Pu WT, Stuart JM, Seidman CE, Seidman JG, Heyn H, Bruneau BG. Dev Cell. 2021 Feb 8;56(3):292-309.e9. doi: 10.1016/j.devcel.2020.11.020. Epub 2020 Dec 14. PubMed
CRISPR-Mediated Mutagenesis of Long Noncoding RNAs. Yamazaki T, Hirose T. Methods Mol Biol. 2021;2254:283-303. doi: 10.1007/978-1-0716-1158-6_18. PubMed
An activity-dependent local transport regulation via degradation and synthesis of KIF17 underlying cognitive flexibility. Iwata S, Morikawa M, Takei Y, Hirokawa N. Sci Adv. 2020 Dec 16;6(51). pii: 6/51/eabc8355. doi: 10.1126/sciadv.abc8355. Print 2020 Dec. PubMed
Reconstitution of the oocyte transcriptional network with transcription factors. Hamazaki N, Kyogoku H, Araki H, Miura F, Horikawa C, Hamada N, Shimamoto S, Hikabe O, Nakashima K, Kitajima TS, Ito T, Leitch HG, Hayashi K. Nature. 2021 Jan;589(7841):264-269. doi: 10.1038/s41586-020-3027-9. Epub 2020 Dec 16. PubMed
SNAP23 deficiency causes severe brain dysplasia through the loss of radial glial cell polarity. Kunii M, Noguchi Y, Yoshimura SI, Kanda S, Iwano T, Avriyanti E, Atik N, Sato T, Sato K, Ogawa M, Harada A. J Cell Biol. 2021 Jan 4;220(1):e201910080. doi: 10.1083/jcb.201910080. PubMed
Competition between PRC2.1 and 2.2 subcomplexes regulates PRC2 chromatin occupancy in human stem cells. Youmans DT, Gooding AR, Dowell RD, Cech TR. Mol Cell. 2021 Feb 4;81(3):488-501.e9. doi: 10.1016/j.molcel.2020.11.044. Epub 2020 Dec 17. PubMed Associated Plasmids pDY156: SUZ12 px330 sgRNA Cas9 plasmid pDY153: Flag(SUZ12) WT HDR plasmid
Lipid-derived electrophiles mediate the effects of chemotherapeutic topoisomerase I poisons. Flor A, Wolfgeher D, Li J, Hanakahi LA, Kron SJ. Cell Chem Biol. 2021 Jun 17;28(6):776-787.e8. doi: 10.1016/j.chembiol.2020.11.011. Epub 2020 Dec 21. PubMed
Establishing a human embryonic stem cell clone with a heterozygous mutation in the DGCR8 gene. Ree D, Borsy A, Fothi A, Orban TI, Varady G, Erdei Z, Sarkadi B, Rethelyi J, Varga N, Apati A. Stem Cell Res. 2020 Dec 22;50:102134. doi: 10.1016/j.scr.2020.102134. PubMed
Chromothripsis drives the evolution of gene amplification in cancer. Shoshani O, Brunner SF, Yaeger R, Ly P, Nechemia-Arbely Y, Kim DH, Fang R, Castillon GA, Yu M, Li JSZ, Sun Y, Ellisman MH, Ren B, Campbell PJ, Cleveland DW. Nature. 2021 Mar;591(7848):137-141. doi: 10.1038/s41586-020-03064-z. Epub 2020 Dec 23. PubMed
Deneddylation by SENP8 restricts hepatitis B virus propagation. Chen DV, Suzuki T, Itoh Y, Maeda Y, Hirano J, Haga S, Zhang H, Ito D, Matsuura Y, Okamoto T. Microbiol Immunol. 2021 Mar;65(3):125-135. doi: 10.1111/1348-0421.12874. Epub 2021 Feb 24. PubMed
Histone demethylase KDM5A promotes tumorigenesis of osteosarcoma tumor. Peng D, Lin B, Xie M, Zhang P, Guo Q, Li Q, Gu Q, Yang S, Sen L. Cell Death Discov. 2021 Jan 12;7(1):9. doi: 10.1038/s41420-020-00396-7. PubMed
Physiological role of the 3'IgH CBEs super-anchor in antibody class switching. Zhang X, Yoon HS, Chapdelaine-Williams AM, Kyritsis N, Alt FW. Proc Natl Acad Sci U S A. 2021 Jan 19;118(3). pii: 2024392118. doi: 10.1073/pnas.2024392118. PubMed
Knockout of the HMG domain of the porcine SRY gene causes sex reversal in gene-edited pigs. Kurtz S, Lucas-Hahn A, Schlegelberger B, Gohring G, Niemann H, Mettenleiter TC, Petersen B. Proc Natl Acad Sci U S A. 2021 Jan 12;118(2). pii: 2008743118. doi: 10.1073/pnas.2008743118. PubMed
Induction of spontaneous human neocentromere formation and long-term maturation. Murillo-Pineda M, Valente LP, Dumont M, Mata JF, Fachinetti D, Jansen LET. J Cell Biol. 2021 Mar 1;220(3). pii: 211684. doi: 10.1083/jcb.202007210. PubMed
Acute perturbation strategies in interrogating RNA polymerase II elongation factor function in gene expression. Zheng B, Aoi Y, Shah AP, Iwanaszko M, Das S, Rendleman EJ, Zha D, Khan N, Smith ER, Shilatifard A. Genes Dev. 2021 Feb 1;35(3-4):273-285. doi: 10.1101/gad.346106.120. Epub 2021 Jan 14. PubMed
Functional Evaluation of a Rare Variant c.516G>C (p.Trp172Cys) in the GJB2 (Connexin 26) Gene Associated with Nonsyndromic Hearing Loss. Maslova EA, Orishchenko KE, Posukh OL. Biomolecules. 2021 Jan 5;11(1). pii: biom11010061. doi: 10.3390/biom11010061. PubMed
Dual Site-Specific Chemoenzymatic Antibody Fragment Conjugation Using CRISPR-Based Hybridoma Engineering. Le Gall CM, van der Schoot JMS, Ramos-Tomillero I, Khalily MP, van Dalen FJ, Wijfjes Z, Smeding L, van Dalen D, Cammarata A, Bonger KM, Figdor CG, Scheeren FA, Verdoes M. Bioconjug Chem. 2021 Feb 17;32(2):301-310. doi: 10.1021/acs.bioconjchem.0c00673. Epub 2021 Jan 21. PubMed
A genome-scale CRISPR screen reveals factors regulating Wnt-dependent renewal of mouse gastric epithelial cells. Murakami K, Terakado Y, Saito K, Jomen Y, Takeda H, Oshima M, Barker N. Proc Natl Acad Sci U S A. 2021 Jan 26;118(4). pii: 2016806118. doi: 10.1073/pnas.2016806118. PubMed
Antigen identification for HLA class I- and HLA class II-restricted T cell receptors using cytokine-capturing antigen-presenting cells. Lee MN, Meyerson M. Sci Immunol. 2021 Jan 22;6(55). pii: 6/55/eabf4001. doi: 10.1126/sciimmunol.abf4001. PubMed Associated Plasmids CRISPR_HLA_class_I_1 CRISPR_HLA_class_I_2 CRISPR_HLA_DPA CRISPR_HLA_DPB CRISPR_HLA_DQA More ...
delete Condon K. na. PubMed
Improved efficiency of genome editing by constitutive expression of Cas9 endonuclease in genetically-modified mice. Ghassemi B, Jamalkhah M, Shokri G, Kehtari M, Soleimani M, Shamsara M, Kiani J. 3 Biotech. 2021 Feb;11(2):56. doi: 10.1007/s13205-020-02580-z. Epub 2021 Jan 11. PubMed
CRISPR editing of the GLI1 first intron abrogates GLI1 expression and differentially alters lineage commitment. Galat Y, Gu H, Perepitchka M, Taylor R, Yoon JW, Glukhova XA, Li XN, Beletsky IP, Walterhouse DO, Galat V, Iannaccone PM. Stem Cells. 2021 May;39(5):564-580. doi: 10.1002/stem.3341. Epub 2021 Feb 22. PubMed
Allele-specific editing ameliorates dominant retinitis pigmentosa in a transgenic mouse model. Patrizi C, Llado M, Benati D, Iodice C, Marrocco E, Guarascio R, Surace EM, Cheetham ME, Auricchio A, Recchia A. Am J Hum Genet. 2021 Feb 4;108(2):295-308. doi: 10.1016/j.ajhg.2021.01.006. Epub 2021 Jan 27. PubMed
Analysis of disease model iPSCs derived from patients with a novel Fanconi anemia-like IBMFS ADH5/ALDH2 deficiency. Mu A, Hira A, Niwa A, Osawa M, Yoshida K, Mori M, Okamoto Y, Inoue K, Kondo K, Kanemaki MT, Matsuda T, Ito E, Kojima S, Nakahata T, Ogawa S, Tanaka K, Matsuo K, Saito MK, Takata M. Blood. 2021 Apr 15;137(15):2021-2032. doi: 10.1182/blood.2020009111. PubMed
Complex consequences of Cantu syndrome SUR2 variant R1154Q in genetically modified mice. Zhang H, Hanson A, de Almeida TS, Emfinger C, McClenaghan C, Harter T, Yan Z, Cooper PE, Brown GS, Arakel EC, Mecham RP, Kovacs A, Halabi CM, Schwappach B, Remedi MS, Nichols CG. JCI Insight. 2021 Mar 8;6(5). pii: 145934. doi: 10.1172/jci.insight.145934. PubMed
SIRT1 stabilizes extrachromosomal gene amplification and contributes to repeat-induced gene silencing. Taniguchi R, Utani K, Thakur B, Ishine K, Aladjem MI, Shimizu N. J Biol Chem. 2021 Jan-Jun;296:100356. doi: 10.1016/j.jbc.2021.100356. Epub 2021 Feb 2. PubMed
Functional analysis of novel A20 variants in patients with atypical inflammatory diseases. Kadowaki S, Hashimoto K, Nishimura T, Kashimada K, Kadowaki T, Kawamoto N, Imai K, Okada S, Kanegane H, Ohnishi H. Arthritis Res Ther. 2021 Feb 6;23(1):52. doi: 10.1186/s13075-021-02434-w. PubMed
Mdfi Promotes C2C12 Cell Differentiation and Positively Modulates Fast-to-Slow-Twitch Muscle Fiber Transformation. Huang B, Jiao Y, Zhu Y, Ning Z, Ye Z, Li QX, Hu C, Wang C. Front Cell Dev Biol. 2021 Jan 22;9:605875. doi: 10.3389/fcell.2021.605875. eCollection 2021. PubMed
Mosaic CRISPR-stop enables rapid phenotyping of nonsense mutations in essential genes. Wang G, Li C, He S, Liu Z. Development. 2021 Mar 9;148(5). pii: dev.196899. doi: 10.1242/dev.196899. PubMed
Induction of Mutant Sik3(Sleepy) Allele in Neurons in Late Infancy Increases Sleep Need. Iwasaki K, Fujiyama T, Nakata S, Park M, Miyoshi C, Hotta-Hirashima N, Ikkyu A, Kakizaki M, Sugiyama F, Mizuno S, Abe M, Sakimura K, Takahashi S, Funato H, Yanagisawa M. J Neurosci. 2021 Mar 24;41(12):2733-2746. doi: 10.1523/JNEUROSCI.1004-20.2020. Epub 2021 Feb 8. PubMed
PML-controlled responses in severe congenital neutropenia with ELANE-misfolding mutations. Olofsen PA, Bosch DA, Roovers O, van Strien PMH, de Looper HWJ, Hoogenboezem RM, Barnhoorn S, Mastroberardino PG, Ghazvini M, van der Velden VHJ, Bindels EMJ, de Pater EM, Touw IP. Blood Adv. 2021 Feb 9;5(3):775-786. doi: 10.1182/bloodadvances.2020003214. PubMed
Vasculature-driven stem cell population coordinates tissue scaling in dynamic organs. Ichijo R, Kabata M, Kidoya H, Muramatsu F, Ishibashi R, Abe K, Tsutsui K, Kubo H, Iizuka Y, Kitano S, Miyachi H, Kubota Y, Fujiwara H, Sada A, Yamamoto T, Toyoshima F. Sci Adv. 2021 Feb 10;7(7). pii: 7/7/eabd2575. doi: 10.1126/sciadv.abd2575. Print 2021 Feb. PubMed
NOTCH1-driven UBR7 stimulates nucleotide biosynthesis to promote T cell acute lymphoblastic leukemia. Srivastava S, Sahu U, Zhou Y, Hogan AK, Sathyan KM, Bodner J, Huang J, Wong KA, Khalatyan N, Savas JN, Ntziachristos P, Ben-Sahra I, Foltz DR. Sci Adv. 2021 Jan 27;7(5). pii: 7/5/eabc9781. doi: 10.1126/sciadv.abc9781. Print 2021 Jan. PubMed
A Casz1-NuRD complex regulates temporal identity transitions in neural progenitors. Mattar P, Jolicoeur C, Dang T, Shah S, Clark BS, Cayouette M. Sci Rep. 2021 Feb 16;11(1):3858. doi: 10.1038/s41598-021-83395-7. PubMed
Generation of an EFNB2-2A-mCherry reporter human embryonic stem cell line using CRISPR/Cas9-mediated site-specific homologous recombination. Huang Y, Wu H, Han X, Wu J, Yu M, Zhao ZA, Shen Z, Hu S, Lei W. Stem Cell Res. 2021 Apr;52:102241. doi: 10.1016/j.scr.2021.102241. Epub 2021 Feb 11. PubMed
Pulsed-electromagnetic-field induced osteoblast differentiation requires activation of genes downstream of adenosine receptors A2A and A3. Kar NS, Ferguson D, Zhang N, Waldorff EI, Ryaby JT, DiDonato JA. PLoS One. 2021 Feb 25;16(2):e0247659. doi: 10.1371/journal.pone.0247659. eCollection 2021. PubMed
Blastocyst complementation using Prdm14-deficient rats enables efficient germline transmission and generation of functional mouse spermatids in rats. Kobayashi T, Goto T, Oikawa M, Sanbo M, Yoshida F, Terada R, Niizeki N, Kajitani N, Kazuki K, Kazuki Y, Hochi S, Nakauchi H, Surani MA, Hirabayashi M. Nat Commun. 2021 Feb 26;12(1):1328. doi: 10.1038/s41467-021-21557-x. PubMed
Systematic analysis of factors that improve homologous direct repair (HDR) efficiency in CRISPR/Cas9 technique. Di Stazio M, Foschi N, Athanasakis E, Gasparini P, d'Adamo AP. PLoS One. 2021 Mar 5;16(3):e0247603. doi: 10.1371/journal.pone.0247603. eCollection 2021. PubMed
An optimized CRISPR/Cas9 approach for precise genome editing in neurons. Fang H, Bygrave AM, Roth RH, Johnson RC, Huganir RL. Elife. 2021 Mar 10;10. pii: 65202. doi: 10.7554/eLife.65202. PubMed Associated Plasmids pSG GluA1 (px330:2-guideCas9) pMiniT:SEP-GluA1 TKIT donor pMiniT:myc-GluA1 TKIT donor GluA2 (px330:2-guideCas9) More ...
ZNF91 deletion in human embryonic stem cells leads to ectopic activation of SVA retrotransposons and up-regulation of KRAB zinc finger gene clusters. Haring NL, van Bree EJ, Jordaan WS, Roels JRE, Sotomayor GC, Hey TM, White FTG, Galland MD, Smidt MP, Jacobs FMJ. Genome Res. 2021 Apr;31(4):551-563. doi: 10.1101/gr.265348.120. Epub 2021 Mar 15. PubMed
ACK1 is dispensable for development, skin tumor formation, and breast cancer cell proliferation. Brandao R, Kwa MQ, Yarden Y, Brakebusch C. FEBS Open Bio. 2021 Jun;11(6):1579-1592. doi: 10.1002/2211-5463.13149. Epub 2021 May 2. PubMed
A more efficient CRISPR-Cas12a variant derived from Lachnospiraceae bacterium MA2020. Tran MH, Park H, Nobles CL, Karunadharma P, Pan L, Zhong G, Wang H, He W, Ou T, Crynen G, Sheptack K, Stiskin I, Mou H, Farzan M. Mol Ther Nucleic Acids. 2021 Feb 18;24:40-53. doi: 10.1016/j.omtn.2021.02.012. eCollection 2021 Jun 4. PubMed
Generation of PARP1 gene knockout human embryonic stem cell line using CRISPR/Cas9. Li Y, Li J, Zhou T, Pan G, Huang K. Stem Cell Res. 2021 May;53:102288. doi: 10.1016/j.scr.2021.102288. Epub 2021 Mar 13. PubMed
Serum fatty acid-binding protein 5 is a significant factor in hepatocellular carcinoma progression independent of tissue expression level. Ohira M, Yokoo H, Ogawa K, Fukai M, Kamiyama T, Sakamoto N, Taketomi A. Carcinogenesis. 2021 Jun 21;42(6):794-803. doi: 10.1093/carcin/bgab025. PubMed
Bora phosphorylation substitutes in trans for T-loop phosphorylation in Aurora A to promote mitotic entry. Tavernier N, Thomas Y, Vigneron S, Maisonneuve P, Orlicky S, Mader P, Regmi SG, Van Hove L, Levinson NM, Gasmi-Seabrook G, Joly N, Poteau M, Velez-Aguilera G, Gavet O, Castro A, Dasso M, Lorca T, Sicheri F, Pintard L. Nat Commun. 2021 Mar 26;12(1):1899. doi: 10.1038/s41467-021-21922-w. PubMed
The BAX-binding protein MOAP1 associates with LC3 and promotes closure of the phagophore. Chang HC, Tao RN, Tan CT, Wu YJ, Bay BH, Yu VC. Autophagy. 2021 Nov;17(11):3725-3739. doi: 10.1080/15548627.2021.1896157. Epub 2021 Mar 30. PubMed
USP42 protects ZNRF3/RNF43 from R-spondin-dependent clearance and inhibits Wnt signalling. Giebel N, de Jaime-Soguero A, Garcia Del Arco A, Landry JJM, Tietje M, Villacorta L, Benes V, Fernandez-Saiz V, Acebron SP. EMBO Rep. 2021 May 5;22(5):e51415. doi: 10.15252/embr.202051415. Epub 2021 Mar 30. PubMed
A HIT-trapping strategy for rapid generation of reversible and conditional alleles using a universal donor. Lu H, Liu J, Feng T, Guo Z, Yin Y, Gao F, Cao G, Du X, Wu S. Genome Res. 2021 May;31(5):900-909. doi: 10.1101/gr.271312.120. Epub 2021 Apr 1. PubMed
Generation of a homozygous ALX1 knockout human embryonic stem cell line (WAe001-A-060) by a CRISPR/Cas9 system. Xiao X, Chen Y, Peng L, Zhang T. Stem Cell Res. 2021 May;53:102309. doi: 10.1016/j.scr.2021.102309. Epub 2021 Mar 24. PubMed
Genetic Deletion of Trace-Amine Associated Receptor 9 (TAAR9) in Rats Leads to Decreased Blood Cholesterol Levels. Murtazina RZ, Zhukov IS, Korenkova OM, Popova EA, Kuvarzin SR, Efimova EV, Kubarskaya LG, Batotsyrenova EG, Zolotoverkhaya EA, Vaganova AN, Apryatin SA, Alenina NV, Gainetdinov RR. Int J Mol Sci. 2021 Mar 14;22(6). pii: ijms22062942. doi: 10.3390/ijms22062942. PubMed
Allelic Expression Imbalance Analysis Identified YAP1 Amplification in p53- Dependent Osteosarcoma. Niu G, Bak A, Nusselt M, Zhang Y, Pausch H, Flisikowska T, Schnieke AE, Flisikowski K. Cancers (Basel). 2021 Mar 18;13(6). pii: cancers13061364. doi: 10.3390/cancers13061364. PubMed
Serotonin Transporter Ala276 Mouse: Novel Model to Assess the Neurochemical and Behavioral Impact of Thr276 Phosphorylation In Vivo. Meinke C, Quinlan MA, Paffenroth KC, Harrison FE, Fenollar-Ferrer C, Katamish RM, Stillman I, Ramamoorthy S, Blakely RD. Neurochem Res. 2021 Apr 8. pii: 10.1007/s11064-021-03299-w. doi: 10.1007/s11064-021-03299-w. PubMed
Novel reporter mouse models useful for evaluating in vivo gene editing and for optimization of methods of delivering genome editing tools. Miura H, Imafuku J, Kurosaki A, Sato M, Ma Y, Zhang G, Mizutani A, Kamimura K, Gurumurthy CB, Liu D, Ohtsuka M. Mol Ther Nucleic Acids. 2021 Mar 5;24:325-336. doi: 10.1016/j.omtn.2021.03.003. eCollection 2021 Jun 4. PubMed
The dystonia gene THAP1 controls DNA double-strand break repair choice. Shinoda K, Zong D, Callen E, Wu W, Dumitrache LC, Belinky F, Chari R, Wong N, Ishikawa M, Stanlie A, Multhaupt-Buell T, Sharma N, Ozelius L, Ehrlich M, McKinnon PJ, Nussenzweig A. Mol Cell. 2021 Jun 17;81(12):2611-2624.e10. doi: 10.1016/j.molcel.2021.03.034. Epub 2021 Apr 14. PubMed
Cyclin M2 (CNNM2) knockout mice show mild hypomagnesaemia and developmental defects. Franken GAC, Seker M, Bos C, Siemons LAH, van der Eerden BCJ, Christ A, Hoenderop JGJ, Bindels RJM, Muller D, Breiderhoff T, de Baaij JHF. Sci Rep. 2021 Apr 15;11(1):8217. doi: 10.1038/s41598-021-87548-6. PubMed
The Murine MHC Class II Super Enhancer IA/IE-SE Contains a Functionally Redundant CTCF-Binding Component and a Novel Element Critical for Maximal Expression. Majumder P, Lee JT, Barwick BG, Patterson DG, Bally APR, Scharer CD, Boss JM. J Immunol. 2021 May 1;206(9):2221-2232. doi: 10.4049/jimmunol.2001089. Epub 2021 Apr 16. PubMed
CRISPR/Cas9-Mediated alpha-ENaC Knockout in a Murine Pancreatic beta-Cell Line. Zhang X, Zhao L, Jin R, Li M, Li MS, Li R, Liang X. Front Genet. 2021 Apr 1;12:664799. doi: 10.3389/fgene.2021.664799. eCollection 2021. PubMed
Universal Gene Correction Approaches for beta-hemoglobinopathies Using CRISPR-Cas9 and Adeno-Associated Virus Serotype 6 Donor Templates. Lamsfus-Calle A, Daniel-Moreno A, Urena-Bailen G, Rottenberger J, Raju J, Epting T, Marciano S, Heumos L, Baskaran P, S Antony J, Handgretinger R, Mezger M. CRISPR J. 2021 Apr;4(2):207-222. doi: 10.1089/crispr.2020.0141. PubMed
Optimizing Integration and Expression of Transgenic Bruton's Tyrosine Kinase for CRISPR-Cas9-Mediated Gene Editing of X-Linked Agammaglobulinemia. Gray DH, Villegas I, Long J, Santos J, Keir A, Abele A, Kuo CY, Kohn DB. CRISPR J. 2021 Apr;4(2):191-206. doi: 10.1089/crispr.2020.0080. PubMed
Piccolo is essential for the maintenance of mouse retina but not cochlear hair cell function. Li P, Lin Z, An Y, Lin J, Zhang A, Wang S, Tu H, Ran J, Wang J, Liang Y, Liu Z, Ye C, Fu X, Gao J. Aging (Albany NY). 2021 Apr 21;13(8):11678-11695. doi: 10.18632/aging.202861. Epub 2021 Apr 21. PubMed
Smoothened transduces Hedgehog signals via activity-dependent sequestration of PKA catalytic subunits. Arveseth CD, Happ JT, Hedeen DS, Zhu JF, Capener JL, Klatt Shaw D, Deshpande I, Liang J, Xu J, Stubben SL, Nelson IB, Walker MF, Kawakami K, Inoue A, Krogan NJ, Grunwald DJ, Huttenhain R, Manglik A, Myers BR. PLoS Biol. 2021 Apr 22;19(4):e3001191. doi: 10.1371/journal.pbio.3001191. eCollection 2021 Apr. PubMed
Modeling retinitis pigmentosa through patient-derived retinal organoids. Li YP, Deng WL, Jin ZB. STAR Protoc. 2021 Apr 8;2(2):100438. doi: 10.1016/j.xpro.2021.100438. eCollection 2021 Jun 18. PubMed
Modeling human retinoblastoma using embryonic stem cell-derived retinal organoids. Liu H, Hua ZQ, Jin ZB. STAR Protoc. 2021 Apr 7;2(2):100444. doi: 10.1016/j.xpro.2021.100444. eCollection 2021 Jun 18. PubMed
Mutually Repulsive EphA7-EfnA5 Organize Region-to-Region Corticopontine Projection by Inhibiting Collateral Extension. Iguchi T, Oka Y, Yasumura M, Omi M, Kuroda K, Yagi H, Xie MJ, Taniguchi M, Bastmeyer M, Sato M. J Neurosci. 2021 Jun 2;41(22):4795-4808. doi: 10.1523/JNEUROSCI.0367-20.2021. Epub 2021 Apr 27. PubMed
ZC3H4 restricts non-coding transcription in human cells. Estell C, Davidson L, Steketee PC, Monier A, West S. Elife. 2021 Apr 29;10. pii: 67305. doi: 10.7554/eLife.67305. PubMed
Arpin Regulates Migration Persistence by Interacting with Both Tankyrases and the Arp2/3 Complex. Simanov G, Dang I, Fokin AI, Oguievetskaia K, Campanacci V, Cherfils J, Gautreau AM. Int J Mol Sci. 2021 Apr 16;22(8). pii: ijms22084115. doi: 10.3390/ijms22084115. PubMed
CBFB cooperates with p53 to maintain TAp73 expression and suppress breast cancer. Malik N, Yan H, Yang HH, Ayaz G, DuBois W, Tseng YC, Kim YI, Jiang S, Liu C, Lee M, Huang J. PLoS Genet. 2021 May 4;17(5):e1009553. doi: 10.1371/journal.pgen.1009553. eCollection 2021 May. PubMed
Disruption of entire Cables2 locus leads to embryonic lethality by diminished Rps21 gene expression and enhanced p53 pathway. Dinh TTH, Iseki H, Mizuno S, Iijima-Mizuno S, Tanimoto Y, Daitoku Y, Kato K, Hamada Y, Hasan ASH, Suzuki H, Murata K, Muratani M, Ema M, Kim JD, Ishida J, Fukamizu A, Kato M, Takahashi S, Yagami KI, Wilson V, Arkell RM, Sugiyama F. Elife. 2021 May 5;10. pii: 50346. doi: 10.7554/eLife.50346. PubMed
DNA polymerase gamma mutations that impair holoenzyme stability cause catalytic subunit depletion. Silva-Pinheiro P, Pardo-Hernandez C, Reyes A, Tilokani L, Mishra A, Cerutti R, Li S, Rozsivalova DH, Valenzuela S, Dogan SA, Peter B, Fernandez-Silva P, Trifunovic A, Prudent J, Minczuk M, Bindoff L, Macao B, Zeviani M, Falkenberg M, Viscomi C. Nucleic Acids Res. 2021 May 21;49(9):5230-5248. doi: 10.1093/nar/gkab282. PubMed
MyD88 oligomer size functions as a physical threshold to trigger IL1R Myddosome signaling. Deliz-Aguirre R, Cao F, Gerpott FHU, Auevechanichkul N, Chupanova M, Mun Y, Ziska E, Taylor MJ. J Cell Biol. 2021 Jul 5;220(7). pii: 212080. doi: 10.1083/jcb.202012071. Epub 2021 May 6. PubMed
Opposing roles of E3 ligases TRIM23 and TRIM21 in regulation of ion channel ANO1 protein levels. Cao X, Zhou Z, Tian Y, Liu Z, Cheng KO, Chen X, Hu W, Wong YM, Li X, Zhang H, Hu R, Huang P. J Biol Chem. 2021 Jan-Jun;296:100738. doi: 10.1016/j.jbc.2021.100738. Epub 2021 May 3. PubMed
NSMF promotes the replication stress-induced DNA damage response for genome maintenance. Ju MK, Shin KJ, Lee JR, Khim KW, A Lee E, Ra JS, Kim BG, Jo HS, Yoon JH, Kim TM, Myung K, Choi JH, Kim H, Chae YC. Nucleic Acids Res. 2021 Jun 4;49(10):5605-5622. doi: 10.1093/nar/gkab311. PubMed
The Dual Function of KDM5C in Both Gene Transcriptional Activation and Repression Promotes Breast Cancer Cell Growth and Tumorigenesis. Shen HF, Zhang WJ, Huang Y, He YH, Hu GS, Wang L, Peng BL, Yi J, Li TT, Rong R, Chen XY, Liu JY, Li WJ, Ohgi K, Li SW, Rosenfeld MG, Liu W. Adv Sci (Weinh). 2021 Feb 18;8(9):2004635. doi: 10.1002/advs.202004635. eCollection 2021 May. PubMed
One-step multiplex toolkit for efficient generation of conditional gene silencing human cell lines. Yeung TK, Lau HW, Ma HT, Poon RYC. Mol Biol Cell. 2021 May 12:mbcE21020051. doi: 10.1091/mbc.E21-02-0051. PubMed Associated Plasmids pUHD-SB-AID/Hyg pUHD-SB-AID/Bla pUHD-SB-mAID/Hyg pUHD-SB-mAID/Bla pUHD-SB-mAID/Zeo More ...
PI5P4Ks drive metabolic homeostasis through peroxisome-mitochondria interplay. Ravi A, Palamiuc L, Loughran RM, Triscott J, Arora GK, Kumar A, Tieu V, Pauli C, Reist M, Lew RJ, Houlihan SL, Fellmann C, Metallo C, Rubin MA, Emerling BM. Dev Cell. 2021 Jun 7;56(11):1661-1676.e10. doi: 10.1016/j.devcel.2021.04.019. Epub 2021 May 12. PubMed
Chromosome oscillation promotes Aurora A-dependent Hec1 phosphorylation and mitotic fidelity. Iemura K, Natsume T, Maehara K, Kanemaki MT, Tanaka K. J Cell Biol. 2021 Jul 5;220(7). pii: 212099. doi: 10.1083/jcb.202006116. Epub 2021 May 14. PubMed
Single AAV-mediated CRISPR-Nme2Cas9 efficiently reduces mutant hTTR expression in a transgenic mouse model of transthyretin amyloidosis. Wen J, Cao T, Wu J, Chen Y, Zhi S, Huang Y, Zhen P, Wu G, Aagaard L, Zhong J, Liang P, Huang J. Mol Ther. 2022 Jan 5;30(1):164-174. doi: 10.1016/j.ymthe.2021.05.010. Epub 2021 May 14. PubMed
Genome-wide screening of circadian and non-circadian impact of Neat1 genetic deletion. Jacq A, Becquet D, Bello-Goutierrez MM, Boyer B, Guillen S, Franc JL, Francois-Bellan AM. Comput Struct Biotechnol J. 2021 Apr 21;19:2121-2132. doi: 10.1016/j.csbj.2021.04.022. eCollection 2021. PubMed
Effect of ARTEMIS (DCLRE1C) deficiency and microinjection timing on editing efficiency during somatic cell nuclear transfer and in vitro fertilization using the CRISPR/Cas9 system. Li Y, Adur MK, Wang W, Schultz RB, Hale B, Wierson W, Charley SE, McGrail M, Essner J, Tuggle CK, Ross JW. Theriogenology. 2021 Aug;170:107-116. doi: 10.1016/j.theriogenology.2021.04.003. Epub 2021 May 3. PubMed
MTHFD2 facilitates breast cancer cell proliferation via the AKT signaling pathway. Huang J, Qin Y, Lin C, Huang X, Zhang F. Exp Ther Med. 2021 Jul;22(1):703. doi: 10.3892/etm.2021.10135. Epub 2021 May 2. PubMed
Deficiency of Klc2 Induces Low-Frequency Sensorineural Hearing Loss in C57BL/6 J Mice and Human. Fu X, An Y, Wang H, Li P, Lin J, Yuan J, Yue R, Jin Y, Gao J, Chai R. Mol Neurobiol. 2021 May 20. pii: 10.1007/s12035-021-02422-w. doi: 10.1007/s12035-021-02422-w. PubMed
Chemically defined and xeno-free culture condition for human extended pluripotent stem cells. Liu B, Chen S, Xu Y, Lyu Y, Wang J, Du Y, Sun Y, Liu H, Zhou H, Lai W, Xue A, Yin M, Li C, Bai Y, Xu J, Deng H. Nat Commun. 2021 May 21;12(1):3017. doi: 10.1038/s41467-021-23320-8. PubMed
Organelle tethering, pore formation and SNARE compensation in the late endocytic pathway. Davis LJ, Bright NA, Edgar JR, Parkinson MDJ, Wartosch L, Mantell J, Peden AA, Luzio JP. J Cell Sci. 2021 May 15;134(10). pii: 268392. doi: 10.1242/jcs.255463. Epub 2021 May 27. PubMed
Division of labor of Y-family polymerases in translesion-DNA synthesis for distinct types of DNA damage. Inomata Y, Abe T, Tsuda M, Takeda S, Hirota K. PLoS One. 2021 Jun 1;16(6):e0252587. doi: 10.1371/journal.pone.0252587. eCollection 2021. PubMed
In vitro functional analysis of four variants of human asparagine synthetase. Matsumoto H, Kawashima N, Yamamoto T, Nakama M, Otsuka H, Ago Y, Sasai H, Kubota K, Ozeki M, Kawamoto N, Esaka Y, Ohnishi H. J Inherit Metab Dis. 2021 Sep;44(5):1226-1234. doi: 10.1002/jimd.12408. Epub 2021 Jun 9. PubMed
Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing. Tsukamoto S, Nakade K, Wakabayashi T, Nakashima K, Takami M, Hemmi Y, Kuramochi Y, Shimizu T, Arai Y, Matsuo-Takasaki M, Noguchi M, Nakamura Y, Miwa Y, Hayashi Y. Stem Cell Res. 2021 May;53:102363. doi: 10.1016/j.scr.2021.102363. Epub 2021 Apr 22. PubMed
Establishment and characterization of a human embryonic stem cell line carrying a heterozygous GATA4(T280M) mutation. Ye L, Yu Y, Wang Y, Huang Y, Yu M, Lei W, Tang JM, Hu S. Stem Cell Res. 2021 May;53:102393. doi: 10.1016/j.scr.2021.102393. Epub 2021 May 12. PubMed
CRISPR/Cas9-mediated correction of MITF homozygous point mutation in a Waardenburg syndrome 2A pig model. Yao J, Wang Y, Cao C, Song R, Bi D, Zhang H, Li Y, Qin G, Hou N, Zhang N, Zhang J, Guo W, Yang S, Wang Y, Zhao J. Mol Ther Nucleic Acids. 2021 Apr 16;24:986-999. doi: 10.1016/j.omtn.2021.04.009. eCollection 2021 Jun 4. PubMed
Generation and Characterization of a Cell Type-Specific, Inducible Cre-Driver Line to Study Olfactory Processing. Koldaeva A, Zhang C, Huang YP, Reinert JK, Mizuno S, Sugiyama F, Takahashi S, Soliman T, Matsunami H, Fukunaga I. J Neurosci. 2021 Jul 28;41(30):6449-6467. doi: 10.1523/JNEUROSCI.3076-20.2021. Epub 2021 Jun 7. PubMed
High-fidelity, efficient, and reversible labeling of endogenous proteins using CRISPR-based designer exon insertion. Zhong H, Ceballos CC, Massengill CI, Muniak MA, Ma L, Qin M, Petrie SK, Mao T. Elife. 2021 Jun 8;10. pii: 64911. doi: 10.7554/eLife.64911. PubMed Associated Plasmids pX330_ACTB-i1 sgRNA / hSpCas9 pX330_ACTB-i2 sgRNA / hSpCas9 pX330_ACTB-i3 sgRNA / hSpCas9 pX330_ACTB-i4 sgRNA / hSpCas9 pX330_ACTB-i5 sgRNA / hSpCas9 More ...
Start codon disruption with CRISPR/Cas9 prevents murine Fuchs' endothelial corneal dystrophy. Uehara H, Zhang X, Pereira F, Narendran S, Choi S, Bhuvanagiri S, Liu J, Ravi Kumar S, Bohner A, Carroll L, Archer B, Zhang Y, Liu W, Gao G, Ambati J, Jun AS, Ambati BK. Elife. 2021 Jun 8;10. pii: 55637. doi: 10.7554/eLife.55637. PubMed
Therapeutic liver repopulation by transient acetaminophen selection of gene-modified hepatocytes. Vonada A, Tiyaboonchai A, Nygaard S, Posey J, Peters AM, Winn SR, Cantore A, Naldini L, Harding CO, Grompe M. Sci Transl Med. 2021 Jun 9;13(597). pii: 13/597/eabg3047. doi: 10.1126/scitranslmed.abg3047. PubMed
NORAD-induced Pumilio phase separation is required for genome stability. Elguindy MM, Mendell JT. Nature. 2021 Jul;595(7866):303-308. doi: 10.1038/s41586-021-03633-w. Epub 2021 Jun 9. PubMed
A human isogenic iPSC-derived cell line panel identifies major regulators of aberrant astrocyte proliferation in Down syndrome. Kawatani K, Nambara T, Nawa N, Yoshimatsu H, Kusakabe H, Hirata K, Tanave A, Sumiyama K, Banno K, Taniguchi H, Arahori H, Ozono K, Kitabatake Y. Commun Biol. 2021 Jun 14;4(1):730. doi: 10.1038/s42003-021-02242-7. PubMed
In-depth assessment of the PAM compatibility and editing activities of Cas9 variants. Zhang W, Yin J, Zhang-Ding Z, Xin C, Liu M, Wang Y, Ai C, Hu J. Nucleic Acids Res. 2021 Sep 7;49(15):8785-8795. doi: 10.1093/nar/gkab507. PubMed
Stress granules inhibit fatty acid oxidation by modulating mitochondrial permeability. Amen T, Kaganovich D. Cell Rep. 2021 Jun 15;35(11):109237. doi: 10.1016/j.celrep.2021.109237. PubMed
A reporter system for enriching CRISPR/Cas9 knockout cells in technically challenging settings like patient models. Liu WH, Volse K, Senft D, Jeremias I. Sci Rep. 2021 Jun 16;11(1):12649. doi: 10.1038/s41598-021-91760-9. PubMed
INO80 promotes H2A.Z occupancy to regulate cell fate transition in pluripotent stem cells. Yu H, Wang J, Lackford B, Bennett B, Li JL, Hu G. Nucleic Acids Res. 2021 Jul 9;49(12):6739-6755. doi: 10.1093/nar/gkab476. PubMed
Fusion transcripts FYN-TRAF3IP2 and KHDRBS1-LCK hijack T cell receptor signaling in peripheral T-cell lymphoma, not otherwise specified. Debackere K, Marcelis L, Demeyer S, Vanden Bempt M, Mentens N, Gielen O, Jacobs K, Broux M, Verhoef G, Michaux L, Graux C, Wlodarska I, Gaulard P, de Leval L, Tousseyn T, Cools J, Dierickx D. Nat Commun. 2021 Jun 17;12(1):3705. doi: 10.1038/s41467-021-24037-4. PubMed
DNA-Based Synthetic Growth Factor Surrogates with Fine-Tuned Agonism*. Akiyama M, Ueki R, Yanagawa M, Abe M, Hiroshima M, Sako Y, Sando S. Angew Chem Int Ed Engl. 2021 Jun 17. doi: 10.1002/anie.202105314. PubMed
Generation and validation of APOE knockout human iPSC-derived cerebral organoids. Martens YA, Xu S, Tait R, Li G, Zhao XC, Lu W, Liu CC, Kanekiyo T, Bu G, Zhao J. STAR Protoc. 2021 Jun 5;2(2):100571. doi: 10.1016/j.xpro.2021.100571. eCollection 2021 Jun 18. PubMed
Genetic engineering of porcine endothelial cell lines for evaluation of human-to-pig xenoreactive immune responses. Li P, Walsh JR, Lopez K, Isidan A, Zhang W, Chen AM, Goggins WC, Higgins NG, Liu J, Brutkiewicz RR, Smith LJ, Hara H, Cooper DKC, Ekser B. Sci Rep. 2021 Jun 23;11(1):13131. doi: 10.1038/s41598-021-92543-y. PubMed
Transcriptomic characterization of cancer-testis antigens identifies MAGEA3 as a driver of tumor progression in hepatocellular carcinoma. Craig AJ, Garcia-Lezana T, Ruiz de Galarreta M, Villacorta-Martin C, Kozlova EG, Martins-Filho SN, von Felden J, Ahsen ME, Bresnahan E, Hernandez-Meza G, Labgaa I, D'Avola D, Schwartz M, Llovet JM, Sia D, Thung S, Losic B, Lujambio A, Villanueva A. PLoS Genet. 2021 Jun 24;17(6):e1009589. doi: 10.1371/journal.pgen.1009589. eCollection 2021 Jun. PubMed
LAMP-Seq enables sensitive, multiplexed COVID-19 diagnostics using molecular barcoding. Ludwig KU, Schmithausen RM, Li D, Jacobs ML, Hollstein R, Blumenstock K, Liebing J, Slabicki M, Ben-Shmuel A, Israeli O, Weiss S, Ebert TS, Paran N, Rudiger W, Wilbring G, Feldman D, Lippke B, Ishorst N, Hochfeld LM, Beins EC, Kaltheuner IH, Schmitz M, Wohler A, Dohla M, Sib E, Jentzsch M, Borrajo JD, Strecker J, Reinhardt J, Cleary B, Geyer M, Holzel M, Macrae R, Nothen MM, Hoffmann P, Exner M, Regev A, Zhang F, Schmid-Burgk JL. Nat Biotechnol. 2021 Jun 29. pii: 10.1038/s41587-021-00966-9. doi: 10.1038/s41587-021-00966-9. PubMed
Simplified All-In-One CRISPR-Cas9 Construction for Efficient Genome Editing in Cryptococcus Species. Zhang P, Wang Y, Li C, Ma X, Ma L, Zhu X. J Fungi (Basel). 2021 Jun 24;7(7). pii: jof7070505. doi: 10.3390/jof7070505. PubMed
Role of Sialyl-O-Acetyltransferase CASD1 on GD2 Ganglioside O-Acetylation in Breast Cancer Cells. Cavdarli S, Schroter L, Albers M, Baumann AM, Vicogne D, Le Doussal JM, Muhlenhoff M, Delannoy P, Groux-Degroote S. Cells. 2021 Jun 11;10(6). pii: cells10061468. doi: 10.3390/cells10061468. PubMed
The CRISPR/Cas9 Minipig-A Transgenic Minipig to Produce Specific Mutations in Designated Tissues. Berthelsen MF, Riedel M, Cai H, Skaarup SH, Alstrup AKO, Dagnaes-Hansen F, Luo Y, Jensen UB, Hager H, Liu Y, Callesen H, Vendelbo MH, Jakobsen JE, Thomsen MK. Cancers (Basel). 2021 Jun 16;13(12). pii: cancers13123024. doi: 10.3390/cancers13123024. PubMed Associated Plasmids LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR AAV_3xgRNA;PTENA, p53B,SMAD4A_CAG_FlPO_synthPA
Loss of peptide:N-glycanase causes proteasome dysfunction mediated by a sugar-recognizing ubiquitin ligase. Yoshida Y, Asahina M, Murakami A, Kawawaki J, Yoshida M, Fujinawa R, Iwai K, Tozawa R, Matsuda N, Tanaka K, Suzuki T. Proc Natl Acad Sci U S A. 2021 Jul 6;118(27). pii: 2102902118. doi: 10.1073/pnas.2102902118. PubMed
Optical Control of Genome Editing by Photoactivatable Cas9. Otabe T, Nihongaki Y, Sato M. Methods Mol Biol. 2021;2312:225-233. doi: 10.1007/978-1-0716-1441-9_13. PubMed
Rationally Designed Transgene-Encoded Cell-Surface Polypeptide Tag for Multiplexed Programming of CAR T-cell Synthetic Outputs. Johnson AJ, Wei J, Rosser JM, Kunkele A, Chang CA, Reid AN, Jensen MC. Cancer Immunol Res. 2021 Sep;9(9):1047-1060. doi: 10.1158/2326-6066.CIR-20-0470. Epub 2021 Jul 9. PubMed
Myomegalin regulates Hedgehog pathway by controlling PDE4D at the centrosome. Peng H, Zhang J, Ya A, Ma W, Villa S, Sukenik S, Ge X. Mol Biol Cell. 2021 Sep 1;32(19):1807-1817. doi: 10.1091/mbc.E21-02-0064. Epub 2021 Jul 14. PubMed
HIF-1alpha affects sensitivity of murine squamous cell carcinoma to boron neutron capture therapy with BPA. Sanada Y, Takata T, Tanaka H, Sakurai Y, Watanabe T, Suzuki M, Masunaga SI. Int J Radiat Biol. 2021 Jul 26:1-9. doi: 10.1080/09553002.2021.1956004. PubMed
Generation of Transgenic Rat Embryonic Stem Cells Using the CRISPR/Cpf1 System for Inducible Gene Knockout. Sherstyuk VV, Zakian SM. Biochemistry (Mosc). 2021 Jul;86(7):843-851. doi: 10.1134/S0006297921070051. PubMed
Modeling High-grade serous ovarian carcinoma using a combination of in vivo fallopian tube electroporation and CRISPR-Cas9-mediated genome editing. Teng K, Ford MJ, Harwalkar K, Li Y, Pacis AS, Farnell D, Yamanaka N, Wang YC, Badescu D, Ton Nu TN, Ragoussis J, Huntsman DG, Arseneau J, Yamanaka Y. Cancer Res. 2021 Jul 23. pii: 0008-5472.CAN-20-1518. doi: 10.1158/0008-5472.CAN-20-1518. PubMed
A novel mouse line with epididymal initial segment-specific expression of Cre recombinase driven by the endogenous Lcn9 promoter. Gong QQ, Wang X, Dou ZL, Zhang KY, Liu XG, Gao JG, Sun XY. PLoS One. 2021 Jul 26;16(7):e0254802. doi: 10.1371/journal.pone.0254802. eCollection 2021. PubMed
Rlf-Mycl gene fusion drives tumorigenesis and metastasis in a mouse model of small cell lung cancer. Ciampricotti M, Karakousi T, Richards AL, Quintanal-Villalonga A, Karatza A, Caeser R, Costa EA, Allaj V, Manoj P, Spainhower KB, Kombak FE, Sanchez-Rivera FJ, Jaspers JE, Zavitsanou AM, Maddalo D, Ventura A, Rideout WM, Akama-Garren EH, Jacks T, Donoghue MTA, Sen T, Oliver TG, Poirier JT, Papagiannakopoulos T, Rudin CM. Cancer Discov. 2021 Aug 3. pii: 2159-8290.CD-21-0441. doi: 10.1158/2159-8290.CD-21-0441. PubMed
CRISPR/Cas9 nanoeditor of double knockout large fragments of E6 and E7 oncogenes for reversing drugs resistance in cervical cancer. Li X, Guo M, Hou B, Zheng B, Wang Z, Huang M, Xu Y, Chang J, Wang T. J Nanobiotechnology. 2021 Aug 5;19(1):231. doi: 10.1186/s12951-021-00970-w. PubMed
Generation of R272Q, S156A and K572R RHOT1/Miro1 point mutations in iPSCs from a healthy individual using FACS-assisted CRISPR/Cas9 genome editing. Schwarz L, Casadei N, Fitzgerald JC. Stem Cell Res. 2021 Aug;55:102469. doi: 10.1016/j.scr.2021.102469. Epub 2021 Jul 25. PubMed
CTCF is a barrier for 2C-like reprogramming. Olbrich T, Vega-Sendino M, Tillo D, Wu W, Zolnerowich N, Pavani R, Tran AD, Domingo CN, Franco M, Markiewicz-Potoczny M, Pegoraro G, FitzGerald PC, Kruhlak MJ, Lazzerini-Denchi E, Nora EP, Nussenzweig A, Ruiz S. Nat Commun. 2021 Aug 11;12(1):4856. doi: 10.1038/s41467-021-25072-x. PubMed
Knock-in of the duck retinoic acid-inducible gene I (RIG-I) into the Mx gene in DF-1 cells enables both stable and immune response-dependent RIG-I expression. Ichikawa K, Motoe Y, Ezaki R, Matsuzaki M, Horiuchi H. Biochem Biophys Rep. 2021 Jul 26;27:101084. doi: 10.1016/j.bbrep.2021.101084. eCollection 2021 Sep. PubMed
Fast and Efficient Generation of Isogenic Induced Pluripotent Stem Cell Lines Using Adenine Base Editing. Nami F, Ramezankhani R, Vandenabeele M, Vervliet T, Vogels K, Urano F, Verfaillie C. CRISPR J. 2021 Aug;4(4):502-518. doi: 10.1089/crispr.2021.0006. PubMed
Targeted regulation of transcription in primary cells using CRISPRa and CRISPRi. Jensen TI, Mikkelsen NS, Gao Z, Fosselteder J, Pabst G, Axelgaard E, Laustsen A, Konig S, Reinisch A, Bak RO. Genome Res. 2021 Nov;31(11):2120-2130. doi: 10.1101/gr.275607.121. Epub 2021 Aug 18. PubMed Associated Plasmids dSpCas9-VPR KRAB-dSpCas9 dSaCas9-VPR
ECHDC1 knockout mice accumulate ethyl-branched lipids and excrete abnormal intermediates of branched-chain fatty acid metabolism. Dewulf JP, Paquay S, Marbaix E, Achouri Y, Van Schaftingen E, Bommer GT. J Biol Chem. 2021 Oct;297(4):101083. doi: 10.1016/j.jbc.2021.101083. Epub 2021 Aug 19. PubMed
Generation of an ACTA2-EGFP reporter human induced pluripotent stem cell line, KITi001-C-41, using CRISPR/Cas9-mediated homologous recombination. Jo S, Kim JW, Noh H, Kim H, Kim JH, Park HJ. Stem Cell Res. 2021 Oct;56:102524. doi: 10.1016/j.scr.2021.102524. Epub 2021 Aug 30. PubMed
CRISPR/Cas9-Mediated in vivo Genetic Correction in a Mouse Model of Hemophilia A. Luo S, Li Z, Dai X, Zhang R, Liang Z, Li W, Zeng M, Su J, Wang J, Liang X, Wu Y, Liang D. Front Cell Dev Biol. 2021 Aug 16;9:672564. doi: 10.3389/fcell.2021.672564. eCollection 2021. PubMed
LATE-a novel sensitive cell-based assay for the study of CRISPR/Cas9-related long-term adverse treatment effects. Glow D, Meyer S, Garcia Roldan I, Akingunsade LM, Riecken K, Fehse B. Mol Ther Methods Clin Dev. 2021 Jul 29;22:249-262. doi: 10.1016/j.omtm.2021.07.004. eCollection 2021 Sep 10. PubMed
Ciliary GPCR-based transcriptome as a key regulator of cilia length control. Kobayashi Y, Tomoshige S, Imakado K, Sekino Y, Koganezawa N, Shirao T, Diniz GB, Miyamoto T, Saito Y. FASEB Bioadv. 2021 Jul 5;3(9):744-767. doi: 10.1096/fba.2021-00029. eCollection 2021 Sep. PubMed
ACBD3 modulates KDEL receptor interaction with PKA for its trafficking via tubulovesicular carrier. Yue X, Qian Y, Zhu L, Gim B, Bao M, Jia J, Jing S, Wang Y, Tan C, Bottanelli F, Ziltener P, Choi S, Hao P, Lee I. BMC Biol. 2021 Sep 7;19(1):194. doi: 10.1186/s12915-021-01137-7. PubMed
Clinical consequences of BRCA2 hypomorphism. Castells-Roca L, Gutierrez-Enriquez S, Bonache S, Bogliolo M, Carrasco E, Aza-Carmona M, Montalban G, Munoz-Subirana N, Pujol R, Cruz C, Llop-Guevara A, Ramirez MJ, Saura C, Lasa A, Serra V, Diez O, Balmana J, Surralles J. NPJ Breast Cancer. 2021 Sep 9;7(1):117. doi: 10.1038/s41523-021-00322-9. PubMed
Efficient biallelic knock-in in mouse embryonic stem cells by in vivo-linearization of donor and transient inhibition of DNA polymerase theta/DNA-PK. Arai D, Nakao Y. Sci Rep. 2021 Sep 13;11(1):18132. doi: 10.1038/s41598-021-97579-8. PubMed
MAD2L2 dimerization and TRIP13 control shieldin activity in DNA repair. de Krijger I, Fohr B, Perez SH, Vincendeau E, Serrat J, Thouin AM, Susvirkar V, Lescale C, Paniagua I, Hoekman L, Kaur S, Altelaar M, Deriano L, Faesen AC, Jacobs JJL. Nat Commun. 2021 Sep 14;12(1):5421. doi: 10.1038/s41467-021-25724-y. PubMed
Tumor cell intrinsic Toll-like receptor 4 signaling promotes melanoma progression and metastatic dissemination. Rogava M, Braun AD, van der Sluis TC, Shridhar N, Tuting T, Gaffal E. Int J Cancer. 2022 Jan 1;150(1):142-151. doi: 10.1002/ijc.33804. Epub 2021 Sep 29. PubMed
The CRL4DTL E3 ligase induces degradation of the DNA replication initiation factor TICRR/TRESLIN specifically during S phase. Wittig KA, Sansam CG, Noble TD, Goins D, Sansam CL. Nucleic Acids Res. 2021 Oct 11;49(18):10507-10523. doi: 10.1093/nar/gkab805. PubMed
UQCRC1 engages cytochrome c for neuronal apoptotic cell death. Hung YC, Huang KL, Chen PL, Li JL, Lu SH, Chang JC, Lin HY, Lo WC, Huang SY, Lee TT, Lin TY, Imai Y, Hattori N, Liu CS, Tsai SY, Chen CH, Lin CH, Chan CC. Cell Rep. 2021 Sep 21;36(12):109729. doi: 10.1016/j.celrep.2021.109729. PubMed
Replication-dependent cytotoxicity and Spartan-mediated repair of trapped PARP1-DNA complexes. Saha LK, Murai Y, Saha S, Jo U, Tsuda M, Takeda S, Pommier Y. Nucleic Acids Res. 2021 Oct 11;49(18):10493-10506. doi: 10.1093/nar/gkab777. PubMed
Activity-based RNA-modifying enzyme probing reveals DUS3L-mediated dihydrouridylation. Dai W, Li A, Yu NJ, Nguyen T, Leach RW, Wuhr M, Kleiner RE. Nat Chem Biol. 2021 Nov;17(11):1178-1187. doi: 10.1038/s41589-021-00874-8. Epub 2021 Sep 23. PubMed
PEG10 viral aspartic protease domain is essential for the maintenance of fetal capillary structure in the mouse placenta. Shiura H, Ono R, Tachibana S, Kohda T, Kaneko-Ishino T, Ishino F. Development. 2021 Oct 1;148(19). pii: 272286. doi: 10.1242/dev.199564. Epub 2021 Sep 24. PubMed
Dual Effect of Organogermanium Compound THGP on RIG-I-Mediated Viral Sensing and Viral Replication during Influenza a Virus Infection. Baidya S, Nishimoto Y, Sato S, Shimada Y, Sakurai N, Nonaka H, Noguchi K, Kido M, Tadano S, Ishikawa K, Li K, Okubo A, Yamada T, Orba Y, Sasaki M, Sawa H, Miyamoto H, Takada A, Nakamura T, Takaoka A. Viruses. 2021 Aug 24;13(9). pii: v13091674. doi: 10.3390/v13091674. PubMed
The modified mitochondrial outer membrane carrier MTCH2 links mitochondrial fusion to lipogenesis. Labbe K, Mookerjee S, Le Vasseur M, Gibbs E, Lerner C, Nunnari J. J Cell Biol. 2021 Nov 1;220(11). pii: 212670. doi: 10.1083/jcb.202103122. Epub 2021 Sep 29. PubMed
Localization of KRAS downstream target ARL4C to invasive pseudopods accelerates pancreatic cancer cell invasion. Harada A, Matsumoto S, Yasumizu Y, Shojima K, Akama T, Eguchi H, Kikuchi A. Elife. 2021 Sep 30;10. pii: 66721. doi: 10.7554/eLife.66721. PubMed
MYC Hyperactivates Wnt Signaling in APC/CTNNB1-Mutated Colorectal Cancer Cells through miR-92a-Dependent Repression of DKK3. Sehgal P, Lanauze C, Wang X, Hayer KE, Torres-Diz M, Leu NA, Sela Y, Stanger BZ, Lengner CJ, Thomas-Tikhonenko A. Mol Cancer Res. 2021 Dec;19(12):2003-2014. doi: 10.1158/1541-7786.MCR-21-0666. Epub 2021 Sep 30. PubMed
The ETS transcription factor ERF controls the exit from the naive pluripotent state in a MAPK-dependent manner. Vega-Sendino M, Olbrich T, Tillo D, Tran AD, Domingo CN, Franco M, FitzGerald PC, Kruhlak MJ, Ruiz S. Sci Adv. 2021 Oct;7(40):eabg8306. doi: 10.1126/sciadv.abg8306. Epub 2021 Oct 1. PubMed
Regulation of prefrontal patterning and connectivity by retinoic acid. Shibata M, Pattabiraman K, Lorente-Galdos B, Andrijevic D, Kim SK, Kaur N, Muchnik SK, Xing X, Santpere G, Sousa AMM, Sestan N. Nature. 2021 Oct;598(7881):483-488. doi: 10.1038/s41586-021-03953-x. Epub 2021 Oct 1. PubMed
Hominini-specific regulation of CBLN2 increases prefrontal spinogenesis. Shibata M, Pattabiraman K, Muchnik SK, Kaur N, Morozov YM, Cheng X, Waxman SG, Sestan N. Nature. 2021 Oct;598(7881):489-494. doi: 10.1038/s41586-021-03952-y. Epub 2021 Oct 1. PubMed
Defective Lipid Droplet-Lysosome Interaction Causes Fatty Liver Disease as Evidenced by Human Mutations in TMEM199 and CCDC115. Larsen LE, van den Boogert MAW, Rios-Ocampo WA, Jansen JC, Conlon D, Chong PLE, Levels JHM, Eilers RE, Sachdev VV, Zelcer N, Raabe T, He M, Hand NJ, Drenth JPH, Rader DJ, Stroes ESG, Lefeber DJ, Jonker JW, Holleboom AG. Cell Mol Gastroenterol Hepatol. 2022;13(2):583-597. doi: 10.1016/j.jcmgh.2021.09.013. Epub 2021 Oct 7. PubMed
IRAK1-dependent Regnase-1-14-3-3 complex formation controls Regnase-1-mediated mRNA decay. Akaki K, Ogata K, Yamauchi Y, Iwai N, Tse KM, Hia F, Mochizuki A, Ishihama Y, Mino T, Takeuchi O. Elife. 2021 Oct 12;10. pii: 71966. doi: 10.7554/eLife.71966. PubMed
Stimulation of motilin secretion by bile, free fatty acids, and acidification in human duodenal organoids. Miedzybrodzka EL, Foreman RE, Lu VB, George AL, Smith CA, Larraufie P, Kay RG, Goldspink DA, Reimann F, Gribble FM. Mol Metab. 2021 Dec;54:101356. doi: 10.1016/j.molmet.2021.101356. Epub 2021 Oct 15. PubMed
Reduction in O-glycome induces differentially glycosylated CD44 to promote stemness and metastasis in pancreatic cancer. Leon F, Seshacharyulu P, Nimmakayala RK, Chugh S, Karmakar S, Nallasamy P, Vengoji R, Rachagani S, Cox JL, Mallya K, Batra SK, Ponnusamy MP. Oncogene. 2022 Jan;41(1):57-71. doi: 10.1038/s41388-021-02047-2. Epub 2021 Oct 21. PubMed
Establishment of a pig CRISPR/Cas9 knockout library for functional gene screening in pig cells. Yu C, Zhong H, Yang X, Li G, Wu Z, Yang H. Biotechnol J. 2021 Oct 27:e2100408. doi: 10.1002/biot.202100408. PubMed
The role of MORC3 in silencing transposable elements in mouse embryonic stem cells. Desai VP, Chouaref J, Wu H, Pastor WA, Kan RL, Oey HM, Li Z, Ho J, Vonk KKD, San Leon Granado D, Christopher MA, Clark AT, Jacobsen SE, Daxinger L. Epigenetics Chromatin. 2021 Oct 27;14(1):49. doi: 10.1186/s13072-021-00420-9. PubMed
A Simple Method that Combines CRISPR and AID to Quickly Generate Conditional Knockouts for Essential Genes in Various Vertebrate Cell Lines. Nishimura K, Fukagawa T. Methods Mol Biol. 2022;2377:109-122. doi: 10.1007/978-1-0716-1720-5_6. PubMed
A bipartite element with allele-specific functions safeguards DNA methylation imprints at the Dlk1-Dio3 locus. Aronson BE, Scourzic L, Shah V, Swanzey E, Kloetgen A, Polyzos A, Sinha A, Azziz A, Caspi I, Li J, Pelham-Webb B, Glenn RA, Vierbuchen T, Wichterle H, Tsirigos A, Dawlaty MM, Stadtfeld M, Apostolou E. Dev Cell. 2021 Nov 22;56(22):3052-3065.e5. doi: 10.1016/j.devcel.2021.10.004. Epub 2021 Oct 27. PubMed
Anti-apoptotic MCL1 Protein Represents Critical Survival Molecule for Most Burkitt Lymphomas and BCL2-negative Diffuse Large B-cell Lymphomas. Klanova M, Kazantsev D, Pokorna E, Zikmund T, Karolova J, Behounek M, Renesova N, Sovilj D, Kelemen CD, Helman K, Jaksa R, Havranek O, Andera L, Trneny M, Klener P. Mol Cancer Ther. 2022 Jan;21(1):89-99. doi: 10.1158/1535-7163.MCT-21-0511. Epub 2021 Nov 2. PubMed
Reprogramming of the heavy-chain CDR3 regions of a human antibody repertoire. Ou T, He W, Quinlan BD, Guo Y, Tran MH, Karunadharma P, Park H, Davis-Gardner ME, Yin Y, Zhang X, Wang H, Zhong G, Farzan M. Mol Ther. 2022 Jan 5;30(1):184-197. doi: 10.1016/j.ymthe.2021.10.027. Epub 2021 Nov 2. PubMed
Systemic biodistribution and hepatocyte-specific gene editing with CRISPR/Cas9 using hyaluronic acid-based nanoparticles. Francis C, Wroblewska L, Pegman P, Amiji M. Nanomedicine. 2022 Feb;40:102488. doi: 10.1016/j.nano.2021.102488. Epub 2021 Nov 5. PubMed
Branched ubiquitin chain binding and deubiquitination by UCH37 facilitate proteasome clearance of stress-induced inclusions. Song A, Hazlett Z, Abeykoon D, Dortch J, Dillon A, Curtiss J, Martinez SB, Hill CP, Yu C, Huang L, Fushman D, Cohen RE, Yao T. Elife. 2021 Nov 11;10. pii: 72798. doi: 10.7554/eLife.72798. PubMed
Phase-separated protein droplets of amyotrophic lateral sclerosis-associated p62/SQSTM1 mutants show reduced inner fluidity. Faruk MO, Ichimura Y, Kageyama S, Komatsu-Hirota S, El-Gowily AH, Sou YS, Koike M, Noda NN, Komatsu M. J Biol Chem. 2021 Dec;297(6):101405. doi: 10.1016/j.jbc.2021.101405. Epub 2021 Nov 12. PubMed
Alternative splicing of NF-YA promotes prostate cancer aggressiveness and represents a new molecular marker for clinical stratification of patients. Belluti S, Semeghini V, Rigillo G, Ronzio M, Benati D, Torricelli F, Reggiani Bonetti L, Carnevale G, Grisendi G, Ciarrocchi A, Dominici M, Recchia A, Dolfini D, Imbriano C. J Exp Clin Cancer Res. 2021 Nov 15;40(1):362. doi: 10.1186/s13046-021-02166-4. PubMed
Deletion of GPR21 improves glucose homeostasis and inhibits the CCL2-CCR2 axis by divergent mechanisms. Riddy DM, Kammoun HL, Murphy AJ, Bosnyak-Gladovic S, De la Fuente Gonzalez R, Merlin J, Ziemann M, Fabb S, Pierce TL, Diepenhorst N, Rueda P, El-Osta A, Gautier JF, Venteclef N, Charman WN, Christopoulos A, Sexton PM, Summers RJ, Febbraio MA, Delerive P, Langmead CJ. BMJ Open Diabetes Res Care. 2021 Nov;9(2). pii: 9/2/e002285. doi: 10.1136/bmjdrc-2021-002285. PubMed
UBR7 acts as a histone chaperone for post-nucleosomal histone H3. Hogan AK, Sathyan KM, Willis AB, Khurana S, Srivastava S, Zasadzinska E, Lee AS, Bailey AO, Gaynes MN, Huang J, Bodner J, Rosencrance CD, Wong KA, Morgan MA, Eagen KP, Shilatifard A, Foltz DR. EMBO J. 2021 Dec 15;40(24):e108307. doi: 10.15252/embj.2021108307. Epub 2021 Nov 17. PubMed
Cullin3 deficiency shapes tumor microenvironment and promotes cholangiocarcinoma in liver-specific Smad4/Pten mutant mice. Zhao M, Quan Y, Zeng J, Lyu X, Wang H, Lei JH, Feng Y, Xu J, Chen Q, Sun H, Xu X, Lu L, Deng CX. Int J Biol Sci. 2021 Oct 11;17(15):4176-4191. doi: 10.7150/ijbs.67379. eCollection 2021. PubMed
A trivalent nucleosome interaction by PHIP/BRWD2 is disrupted in neurodevelopmental disorders and cancer. Morgan MAJ, Popova IK, Vaidya A, Burg JM, Marunde MR, Rendleman EJ, Dumar ZJ, Watson R, Meiners MJ, Howard SA, Khalatyan N, Vaughan RM, Rothbart SB, Keogh MC, Shilatifard A. Genes Dev. 2021 Dec 1;35(23-24):1642-1656. doi: 10.1101/gad.348766.121. Epub 2021 Nov 24. PubMed
ecDNA hubs drive cooperative intermolecular oncogene expression. Hung KL, Yost KE, Xie L, Shi Q, Helmsauer K, Luebeck J, Schopflin R, Lange JT, Chamorro Gonzalez R, Weiser NE, Chen C, Valieva ME, Wong IT, Wu S, Dehkordi SR, Duffy CV, Kraft K, Tang J, Belk JA, Rose JC, Corces MR, Granja JM, Li R, Rajkumar U, Friedlein J, Bagchi A, Satpathy AT, Tjian R, Mundlos S, Bafna V, Henssen AG, Mischel PS, Liu Z, Chang HY. Nature. 2021 Dec;600(7890):731-736. doi: 10.1038/s41586-021-04116-8. Epub 2021 Nov 24. PubMed
An Episomal CRISPR/Cas12a System for Mediating Efficient Gene Editing. Duan N, Tang S, Zeng B, Hu Z, Hu Q, Wu L, Zhou M, Liang D. Life (Basel). 2021 Nov 18;11(11). pii: life11111262. doi: 10.3390/life11111262. PubMed
ELOVL2 promotes cancer progression by inhibiting cell apoptosis in renal cell carcinoma. Tanaka K, Kandori S, Sakka S, Nitta S, Tanuma K, Shiga M, Nagumo Y, Negoro H, Kojima T, Mathis BJ, Shimazui T, Watanabe M, Sato TA, Miyamoto T, Matsuzaka T, Shimano H, Nishiyama H. Oncol Rep. 2022 Feb;47(2). pii: 23. doi: 10.3892/or.2021.8234. Epub 2021 Nov 29. PubMed
CRISPR-Cas9 effectors facilitate generation of single-sex litters and sex-specific phenotypes. Douglas C, Maciulyte V, Zohren J, Snell DM, Mahadevaiah SK, Ojarikre OA, Ellis PJI, Turner JMA. Nat Commun. 2021 Dec 3;12(1):6926. doi: 10.1038/s41467-021-27227-2. PubMed
Loss of Profilin3 Impairs Spermiogenesis by Affecting Acrosome Biogenesis, Autophagy, Manchette Development and Mitochondrial Organization. Umer N, Arevalo L, Phadke S, Lohanadan K, Kirfel G, Sons D, Sofia D, Witke W, Schorle H. Front Cell Dev Biol. 2021 Nov 4;9:749559. doi: 10.3389/fcell.2021.749559. eCollection 2021. PubMed
Involvement of Denervated Midbrain-Derived Factors in the Formation of Ectopic Cortico-Mesencephalic Projection after Hemispherectomy. Chang L, Masada M, Kojima M, Yamamoto N. J Neurosci. 2022 Feb 2;42(5):749-761. doi: 10.1523/JNEUROSCI.1371-21.2021. Epub 2021 Dec 9. PubMed
MYC assembles and stimulates topoisomerases 1 and 2 in a "topoisome". Das SK, Kuzin V, Cameron DP, Sanford S, Jha RK, Nie Z, Rosello MT, Holewinski R, Andresson T, Wisniewski J, Natsume T, Price DH, Lewis BA, Kouzine F, Levens D, Baranello L. Mol Cell. 2022 Jan 6;82(1):140-158.e12. doi: 10.1016/j.molcel.2021.11.016. Epub 2021 Dec 9. PubMed
CTCF-mediated chromatin looping provides a topological framework for the formation of phase-separated transcriptional condensates. Lee R, Kang MK, Kim YJ, Yang B, Shim H, Kim S, Kim K, Yang CM, Min BG, Jung WJ, Lee EC, Joo JS, Park G, Cho WK, Kim HP. Nucleic Acids Res. 2022 Jan 11;50(1):207-226. doi: 10.1093/nar/gkab1242. PubMed
CRISPR-to-Kill (C2K)-Employing the Bacterial Immune System to Kill Cancer Cells. Glow D, Maire CL, Schwarze LI, Lamszus K, Fehse B. Cancers (Basel). 2021 Dec 15;13(24). pii: cancers13246306. doi: 10.3390/cancers13246306. PubMed
Selective suppression of IL-10 transcription by calcineurin in dendritic cells through inactivation of CREB. Lu X, Oh-Hora M, Takeda K, Yamasaki S. Int Immunol. 2022 Mar 25;34(4):197-206. doi: 10.1093/intimm/dxab112. PubMed
TAT-RHIM: a more complex issue than expected. Kolbrink B, Riebeling T, Teiwes NK, Steinem C, Kalbacher H, Kunzendorf U, Krautwald S. Biochem J. 2022 Feb 11;479(3):259-272. doi: 10.1042/BCJ20210677. PubMed
Genome-wide CRISPR/Cas9 library screen identifies PCMT1 as a critical driver of ovarian cancer metastasis. Zhang J, Li Y, Liu H, Zhang J, Wang J, Xia J, Zhang Y, Yu X, Ma J, Huang M, Wang J, Wang L, Li Q, Cui R, Yang W, Xu Y, Feng W. J Exp Clin Cancer Res. 2022 Jan 15;41(1):24. doi: 10.1186/s13046-022-02242-3. PubMed
From Genome Sequencing to CRISPR-Based Genome Editing for Climate-Resilient Forest Trees. Cao HX, Vu GTH, Gailing O. Int J Mol Sci. 2022 Jan 16;23(2). pii: ijms23020966. doi: 10.3390/ijms23020966. PubMed
Construction of a human hTERT RPE-1 cell line with inducible Cre for editing of endogenous genes. Hindul NL, Jhita A, Oprea DG, Hussain TA, Gonchar O, Campillo MAM, O'Regan L, Kanemaki MT, Fry AM, Hirota K, Tanaka K. Biol Open. 2022 Feb 15;11(2). pii: 274087. doi: 10.1242/bio.059056. Epub 2022 Feb 16. PubMed Associated Plasmids pInt-ERCreER
Brahma safeguards canalization of cardiac mesoderm differentiation. Hota SK, Rao KS, Blair AP, Khalilimeybodi A, Hu KM, Thomas R, So K, Kameswaran V, Xu J, Polacco BJ, Desai RV, Chatterjee N, Hsu A, Muncie JM, Blotnick AM, Winchester SAB, Weinberger LS, Huttenhain R, Kathiriya IS, Krogan NJ, Saucerman JJ, Bruneau BG. Nature. 2022 Feb;602(7895):129-134. doi: 10.1038/s41586-021-04336-y. Epub 2022 Jan 26. PubMed
IRF8 is a transcriptional activator of CD37 expression in diffuse large B-cell lymphoma. Elfrink S, Ter Beest M, Janssen L, Baltissen MP, Jansen PWTC, Kenyon AN, Steen RM, de Windt D, Hagemann PM, Hess C, van Spronsen DJ, Hoevenaars B, van der Spek E, Xu-Monette ZY, Young KH, Kaffa C, Bervoets S, van Heek J, Hesius E, de Winde CM, Vermeulen M, van den Brand M, Scheijen B, van Spriel AB. Blood Adv. 2022 Apr 12;6(7):2254-2266. doi: 10.1182/bloodadvances.2021004366. PubMed
shRNAs targeting mouse Adam10 diminish cell response to proinflammatory stimuli independently of Adam10 silencing. Czarnek M, Stalinska K, Sarad K, Bereta J. Biol Open. 2022 Mar 15;11(3). pii: 274200. doi: 10.1242/bio.059092. Epub 2022 Mar 4. PubMed
WIN site inhibition disrupts a subset of WDR5 function. Siladi AJ, Wang J, Florian AC, Thomas LR, Creighton JH, Matlock BK, Flaherty DK, Lorey SL, Howard GC, Fesik SW, Weissmiller AM, Liu Q, Tansey WP. Sci Rep. 2022 Feb 3;12(1):1848. doi: 10.1038/s41598-022-05947-9. PubMed
Suppression of premature transcription termination leads to reduced mRNA isoform diversity and neurodegeneration. LaForce GR, Farr JS, Liu J, Akesson C, Gumus E, Pinkard O, Miranda HC, Johnson K, Sweet TJ, Ji P, Lin A, Coller J, Philippidou P, Wagner EJ, Schaffer AE. Neuron. 2022 Apr 20;110(8):1340-1357.e7. doi: 10.1016/j.neuron.2022.01.018. Epub 2022 Feb 8. PubMed
Recruitment of two Ndc80 complexes via the CENP-T pathway is sufficient for kinetochore functions. Takenoshita Y, Hara M, Fukagawa T. Nat Commun. 2022 Feb 14;13(1):851. doi: 10.1038/s41467-022-28403-8. PubMed
TTR exon-humanized mouse optimal for verifying new therapies for FAP. Li Z, Kanazashi H, Tokashiki Y, Fujikawa R, Okagaki A, Katoh S, Kojima K, Haruna K, Matsushita N, Ishikawa TO, Chen H, Yamamura K. Biochem Biophys Res Commun. 2022 Apr 9;599:69-74. doi: 10.1016/j.bbrc.2022.02.035. Epub 2022 Feb 10. PubMed
Identification and Functional Analysis of the Regulatory Elements in the pHSPA6 Promoter. Jiao S, Bai C, Qi C, Wu H, Hu L, Li F, Yang K, Zhao C, Ouyang H, Pang D, Tang X, Xie Z. Genes (Basel). 2022 Jan 21;13(2):189. doi: 10.3390/genes13020189. PubMed
Characterization and Functional Study of FAM49B Reveals Its Effect on Cell Proliferation in HEK293T Cells. Chen Y, Jiang Y, Lao J, Zhou Y, Su L, Huang X. Genes (Basel). 2022 Feb 21;13(2):388. doi: 10.3390/genes13020388. PubMed
Antiviral Targeting of Varicella Zoster Virus Replication and Neuronal Reactivation Using CRISPR/Cas9 Cleavage of the Duplicated Open Reading Frames 62/71. Wu BW, Yee MB, Goldstein RS, Kinchington PR. Viruses. 2022 Feb 12;14(2). pii: v14020378. doi: 10.3390/v14020378. PubMed
Entamoeba histolytica Develops Resistance to Complement Deposition and Lysis after Acquisition of Human Complement-Regulatory Proteins through Trogocytosis. Miller HW, Tam TSY, Ralston KS. mBio. 2022 Apr 26;13(2):e0316321. doi: 10.1128/mbio.03163-21. Epub 2022 Mar 1. PubMed
Inducible caspase-9 suicide gene under control of endogenous oct4 to safeguard mouse and human pluripotent stem cell therapy. Liu Y, Yang Y, Suo Y, Li C, Chen M, Zheng S, Li H, Tang C, Fan N, Lan T, Zhou J, Li Y, Wang J, Chen H, Zou Q, Lai L. Mol Ther Methods Clin Dev. 2022 Feb 1;24:332-341. doi: 10.1016/j.omtm.2022.01.014. eCollection 2022 Mar 10. PubMed
Disrupted Choline Clearance and Sustained Acetylcholine Release In Vivo by a Common Choline Transporter Coding Variant Associated with Poor Attentional Control in Humans. Donovan E, Avila C, Klausner S, Parikh V, Fenollar-Ferrer C, Blakely RD, Sarter M. J Neurosci. 2022 Apr 20;42(16):3426-3444. doi: 10.1523/JNEUROSCI.1334-21.2022. Epub 2022 Mar 1. PubMed
CRISPR-Mediated Enzyme Fragment Complementation Assay for Quantification of the Stability of Splice Isoforms. Tang Z, Hegde S, Zhao J, Zhu S, Johnson KA, Lorson CL, Wang J. Chembiochem. 2022 May 4;23(9):e202200012. doi: 10.1002/cbic.202200012. Epub 2022 Mar 14. PubMed Associated Plasmids pCI-SMN2-inclusion-GLuc
Rap1 prevents colitogenic Th17 cell expansion and facilitates Treg cell differentiation and distal TCR signaling. Ishihara S, Sato T, Fujikado N, Miyazaki H, Yoshimoto T, Yamamoto H, Fukuda S, Katagiri K. Commun Biol. 2022 Mar 4;5(1):206. doi: 10.1038/s42003-022-03129-x. PubMed
Viable offspring derived from single unfertilized mammalian oocytes. Wei Y, Yang CR, Zhao ZA. Proc Natl Acad Sci U S A. 2022 Mar 22;119(12):e2115248119. doi: 10.1073/pnas.2115248119. Epub 2022 Mar 7. PubMed
Cas9 exo-endonuclease eliminates chromosomal translocations during genome editing. Yin J, Lu R, Xin C, Wang Y, Ling X, Li D, Zhang W, Liu M, Xie W, Kong L, Si W, Wei P, Xiao B, Lee HY, Liu T, Hu J. Nat Commun. 2022 Mar 8;13(1):1204. doi: 10.1038/s41467-022-28900-w. PubMed
A simple protocol to isolate a single human cell PRDX1 knockout generated by CRISPR-Cas9 system. Aouida M, Aljogol D, Ali R, Ramotar D. STAR Protoc. 2022 Mar 4;3(1):101216. doi: 10.1016/j.xpro.2022.101216. eCollection 2022 Mar 18. PubMed
Spatiotemporal reprogramming of differentiated cells underlies regeneration and neoplasia in the intestinal epithelium. Higa T, Okita Y, Matsumoto A, Nakayama S, Oka T, Sugahara O, Koga D, Takeishi S, Nakatsumi H, Hosen N, Robine S, Taketo MM, Sato T, Nakayama KI. Nat Commun. 2022 Mar 21;13(1):1500. doi: 10.1038/s41467-022-29165-z. PubMed
Boosting targeted genome editing using the hei-tag. Thumberger T, Tavhelidse-Suck T, Gutierrez-Triana JA, Cornean A, Medert R, Welz B, Freichel M, Wittbrodt J. Elife. 2022 Mar 25;11. pii: 70558. doi: 10.7554/eLife.70558. PubMed Associated Plasmids pCS2+ heiCas9
A point mutation in human coilin prevents Cajal body formation. Basello DA, Matera AG, Stanek D. J Cell Sci. 2022 Apr 15;135(8). pii: 274900. doi: 10.1242/jcs.259587. Epub 2022 Apr 25. PubMed
Developmental delay and late onset HBSL pathology in hypomorphic Dars1(M256L) mice. Klugmann M, Kalotay E, Delerue F, Ittner LM, Bongers A, Yu J, Morris MJ, Housley GD, Frohlich D. Neurochem Res. 2022 Jul;47(7):1972-1984. doi: 10.1007/s11064-022-03582-4. Epub 2022 Mar 31. PubMed
Rapid Assessment of CRISPR Transfection Efficiency and Enrichment of CRISPR Induced Mutations Using a Dual-Fluorescent Stable Reporter System. Shalaby KE, Aouida M, Gupta V, Ghanem SS, El-Agnaf OMA. Front Genome Ed. 2022 Mar 21;4:854866. doi: 10.3389/fgeed.2022.854866. eCollection 2022. PubMed
Biallelic, Selectable, Knock-in Targeting of CCR5 via CRISPR-Cas9 Mediated Homology Directed Repair Inhibits HIV-1 Replication. Scheller SH, Rashad Y, Saleh FM, Willingham KA, Reilich A, Lin D, Izadpanah R, Alt EU, Braun SE. Front Immunol. 2022 Mar 21;13:821190. doi: 10.3389/fimmu.2022.821190. eCollection 2022. PubMed
DELE1 tracks perturbed protein import and processing in human mitochondria. Fessler E, Krumwiede L, Jae LT. Nat Commun. 2022 Apr 6;13(1):1853. doi: 10.1038/s41467-022-29479-y. PubMed
Analysis of Nucleoporin Function Using Inducible Degron Techniques. Aksenova V, Arnaoutov A, Dasso M. Methods Mol Biol. 2022;2502:129-150. doi: 10.1007/978-1-0716-2337-4_9. PubMed
Repo-Man/protein phosphatase 1 SUMOylation mediates binding to lamin A and serine 22 dephosphorylation. Huguet F, Gokan E, Foster HA, Amin HA, Vagnarelli P. Open Biol. 2022 Apr;12(4):220017. doi: 10.1098/rsob.220017. Epub 2022 Apr 13. PubMed
Protection of nascent DNA at stalled replication forks is mediated by phosphorylation of RIF1 intrinsically disordered region. Balasubramanian S, Andreani M, Andrade JG, Saha T, Sundaravinayagam D, Garzon J, Zhang W, Popp O, Hiraga SI, Rahjouei A, Rosen DB, Mertins P, Chait BT, Donaldson AD, Di Virgilio M. Elife. 2022 Apr 13;11:e75047. doi: 10.7554/eLife.75047. PubMed
USP25 promotes pathological HIF-1-driven metabolic reprogramming and is a potential therapeutic target in pancreatic cancer. Nelson JK, Thin MZ, Evan T, Howell S, Wu M, Almeida B, Legrave N, Koenis DS, Koifman G, Sugimoto Y, Llorian Sopena M, MacRae J, Nye E, Howell M, Snijders AP, Prachalias A, Zen Y, Sarker D, Behrens A. Nat Commun. 2022 Apr 19;13(1):2070. doi: 10.1038/s41467-022-29684-9. PubMed
Mammalian UPF3A and UPF3B can activate nonsense-mediated mRNA decay independently of their exon junction complex binding. Yi Z, Arvola RM, Myers S, Dilsavor CN, Abu Alhasan R, Carter BN, Patton RD, Bundschuh R, Singh G. EMBO J. 2022 May 16;41(10):e109202. doi: 10.15252/embj.2021109202. Epub 2022 Apr 22. PubMed
Identification and characterization of a novel enhancer in the HTLV-1 proviral genome. Matsuo M, Ueno T, Monde K, Sugata K, Tan BJY, Rahman A, Miyazato P, Uchiyama K, Islam S, Katsuya H, Nakajima S, Tokunaga M, Nosaka K, Hata H, Utsunomiya A, Fujisawa JI, Satou Y. Nat Commun. 2022 May 3;13(1):2405. doi: 10.1038/s41467-022-30029-9. PubMed
Reprogramming Mouse Oviduct Epithelial Cells Using In Vivo Electroporation and CRISPR/Cas9-Mediated Genetic Manipulation. Ford MJ, Yamanaka Y. Methods Mol Biol. 2022;2429:367-377. doi: 10.1007/978-1-0716-1979-7_24. PubMed
CRISPR-Cas9 editing of the arginine-vasopressin V1a receptor produces paradoxical changes in social behavior in Syrian hamsters. Taylor JH, Walton JC, McCann KE, Norvelle A, Liu Q, Vander Velden JW, Borland JM, Hart M, Jin C, Huhman KL, Cox DN, Albers HE. Proc Natl Acad Sci U S A. 2022 May 10;119(19):e2121037119. doi: 10.1073/pnas.2121037119. Epub 2022 May 5. PubMed
Targeting double-strand break indel byproducts with secondary guide RNAs improves Cas9 HDR-mediated genome editing efficiencies. Bodai Z, Bishop AL, Gantz VM, Komor AC. Nat Commun. 2022 May 9;13(1):2351. doi: 10.1038/s41467-022-29989-9. PubMed
Genetic Catalytic Inactivation of GRK5 Impairs Cardiac Function in Mice Via Dysregulated P53 Levels. Marzano F, Liccardo D, Elia A, Mucio I, de Lucia C, Lucchese AM, Gao E, Ferrara N, Rapacciuolo A, Paolocci N, Rengo G, Koch WJ, Cannavo A. JACC Basic Transl Sci. 2022 Mar 9;7(4):366-380. doi: 10.1016/j.jacbts.2022.01.001. eCollection 2022 Apr. PubMed
BAG6 prevents the aggregation of neurodegeneration-associated fragments of TDP43. Kasu YAT, Arva A, Johnson J, Sajan C, Manzano J, Hennes A, Haynes J, Brower CS. iScience. 2022 Apr 20;25(5):104273. doi: 10.1016/j.isci.2022.104273. eCollection 2022 May 20. PubMed
Epigenome editing reveals core DNA methylation for imprinting control in the Dlk1-Dio3 imprinted domain. Kojima S, Shiochi N, Sato K, Yamaura M, Ito T, Yamamura N, Goto N, Odamoto M, Kobayashi S, Kimura T, Sekita Y. Nucleic Acids Res. 2022 May 20;50(9):5080-5094. doi: 10.1093/nar/gkac344. PubMed
Can SpRY recognize any PAM in human cells? Ye J, Xi H, Chen Y, Chen Q, Lu X, Lv J, Chen Y, Gu F, Zhao J. J Zhejiang Univ Sci B. 2022 May 15;23(5):382-391. doi: 10.1631/jzus.B2100710. PubMed
CUX2 deficiency causes facilitation of excitatory synaptic transmission onto hippocampus and increased seizure susceptibility to kainate. Suzuki T, Tatsukawa T, Sudo G, Delandre C, Pai YJ, Miyamoto H, Raveau M, Shimohata A, Ohmori I, Hamano SI, Haginoya K, Uematsu M, Takahashi Y, Morimoto M, Fujimoto S, Osaka H, Oguni H, Osawa M, Ishii A, Hirose S, Kaneko S, Inoue Y, Moore AW, Yamakawa K. Sci Rep. 2022 May 17;12(1):6505. doi: 10.1038/s41598-022-10715-w. PubMed
RBM33 directs the nuclear export of transcripts containing GC-rich elements. Thomas A, Rehfeld F, Zhang H, Chang TC, Goodarzi M, Gillet F, Mendell JT. Genes Dev. 2022 May 1;36(9-10):550-565. doi: 10.1101/gad.349456.122. Epub 2022 May 19. PubMed
Labeling Endogenous Proteins Using CRISPR-mediated Insertion of Exon (CRISPIE). Wilson EA, Mao T, Zhong H. Bio Protoc. 2022 Mar 5;12(5):e4343. doi: 10.21769/BioProtoc.4343. eCollection 2022 Mar 5. PubMed
Identification of the CKM Gene as a Potential Muscle-Specific Safe Harbor Locus in Pig Genome. Xiong Y, Zhuang R, Zhao G, Liu Y, Su Y, Wang W, Xi X, Yang Y, Han X, Xie S, Wang H, Li X, Zuo B, Zhao S, Feng Z, Ruan J. Genes (Basel). 2022 May 21;13(5). pii: genes13050921. doi: 10.3390/genes13050921. PubMed
Structure and regulation of the nuclear exosome targeting complex guides RNA substrates to the exosome. Gerlach P, Garland W, Lingaraju M, Salerno-Kochan A, Bonneau F, Basquin J, Jensen TH, Conti E. Mol Cell. 2022 Jul 7;82(13):2505-2518.e7. doi: 10.1016/j.molcel.2022.04.011. Epub 2022 Jun 9. PubMed
Generating a Heat-Tolerance Mouse Model. Liu J, Verma PJ. Methods Mol Biol. 2022;2495:259-272. doi: 10.1007/978-1-0716-2301-5_14. PubMed
Premature transcription termination at the expanded GAA repeats and aberrant alternative polyadenylation contributes to the Frataxin transcriptional deficit in Friedreich's ataxia. Li Y, Li J, Wang J, Zhang S, Giles K, Prakash TP, Rigo F, Napierala JS, Napierala M. Hum Mol Genet. 2022 Oct 10;31(20):3539-3557. doi: 10.1093/hmg/ddac134. PubMed
CRISPR/Cas9induced saturated mutagenesis identifies Rad51 haplotype as a marker of PARP inhibitor sensitivity in breast cancer. Yang H, Wei Y, Zhang Q, Yang Y, Bi X, Yang L, Xiao N, Zang A, Ren L, Li X. Mol Med Rep. 2022 Aug;26(2). pii: 258. doi: 10.3892/mmr.2022.12774. Epub 2022 Jun 17. PubMed
Context-dependent enhancer function revealed by targeted inter-TAD relocation. Bolt CC, Lopez-Delisle L, Hintermann A, Mascrez B, Rauseo A, Andrey G, Duboule D. Nat Commun. 2022 Jun 17;13(1):3488. doi: 10.1038/s41467-022-31241-3. PubMed
Reciprocal regulation of Daxx and PIK3CA promotes colorectal cancer cell growth. Huang YS, Wu CC, Chang CC, Huang SF, Kuo HY, Shih HM. Cell Mol Life Sci. 2022 Jun 19;79(7):367. doi: 10.1007/s00018-022-04399-8. PubMed
Lipolysis of bone marrow adipocytes is required to fuel bone and the marrow niche during energy deficits. Li Z, Bowers E, Zhu J, Yu H, Hardij J, Bagchi DP, Mori H, Lewis KT, Granger K, Schill RL, Romanelli SM, Abrishami S, Hankenson KD, Singer K, Rosen CJ, MacDougald OA. Elife. 2022 Jun 22;11:e78496. doi: 10.7554/eLife.78496. PubMed
Coiled-coil heterodimer-based recruitment of an exonuclease to CRISPR/Cas for enhanced gene editing. Lainscek D, Forstneric V, Mikolic V, Malensek S, Pecan P, Bencina M, Sever M, Podgornik H, Jerala R. Nat Commun. 2022 Jun 23;13(1):3604. doi: 10.1038/s41467-022-31386-1. PubMed
PAM-flexible dual base editor-mediated random mutagenesis and self-activation strategies to improve CRISPRa potency. Lau CH, Huang S, Lam RHW, Tin C. Mol Ther Methods Clin Dev. 2022 May 29;26:26-37. doi: 10.1016/j.omtm.2022.05.005. eCollection 2022 Sep 8. PubMed
The importance of DNAPKcs for blunt DNA end joining is magnified when XLF is weakened. Cisneros-Aguirre M, Lopezcolorado FW, Tsai LJ, Bhargava R, Stark JM. Nat Commun. 2022 Jun 27;13(1):3662. doi: 10.1038/s41467-022-31365-6. PubMed
Aberrant cortical development is driven by impaired cell cycle and translational control in a DDX3X syndrome model. Hoye ML, Calviello L, Poff AJ, Ejimogu NE, Newman CR, Montgomery MD, Ou J, Floor SN, Silver DL. Elife. 2022 Jun 28;11:e78203. doi: 10.7554/eLife.78203. PubMed
An Rtn4/Nogo-A-interacting micropeptide modulates synaptic plasticity with age. Kragness S, Clark Z, Mullin A, Guidry J, Earls LR. PLoS One. 2022 Jun 30;17(6):e0269404. doi: 10.1371/journal.pone.0269404. eCollection 2022. PubMed
CRISPR-Cas12a System With Synergistic Phage Recombination Proteins for Multiplex Precision Editing in Human Cells. Wang C, Xia Q, Zhang Q, Qu Y, Su S, Cheng JKW, Hughes NW, Cong L. Front Cell Dev Biol. 2022 Jun 14;9:719705. doi: 10.3389/fcell.2021.719705. eCollection 2021. PubMed
TET1 dioxygenase is required for FOXA2-associated chromatin remodeling in pancreatic beta-cell differentiation. Li J, Wu X, Ke J, Lee M, Lan Q, Li J, Yu J, Huang Y, Sun DQ, Xie R. Nat Commun. 2022 Jul 7;13(1):3907. doi: 10.1038/s41467-022-31611-x. PubMed
Mast4 determines the cell fate of MSCs for bone and cartilage development. Kim P, Park J, Lee DJ, Mizuno S, Shinohara M, Hong CP, Jeong Y, Yun R, Park H, Park S, Yang KM, Lee MJ, Jang SP, Kim HY, Lee SJ, Song SU, Park KS, Tanaka M, Ohshima H, Cho JW, Sugiyama F, Takahashi S, Jung HS, Kim SJ. Nat Commun. 2022 Jul 8;13(1):3960. doi: 10.1038/s41467-022-31697-3. PubMed
A truncated reverse transcriptase enhances prime editing by split AAV vectors. Gao Z, Ravendran S, Mikkelsen NS, Haldrup J, Cai H, Ding X, Paludan SR, Thomsen MK, Mikkelsen JG, Bak RO. Mol Ther. 2022 Jul 8. pii: S1525-0016(22)00422-1. doi: 10.1016/j.ymthe.2022.07.001. PubMed Associated Plasmids PE_CO PE_CO-Mini-N PE_CO-Mini-C LV-PE_CO-Mini
Mesp1 controls the chromatin and enhancer landscapes essential for spatiotemporal patterning of early cardiovascular progenitors. Lin X, Swedlund B, Ton MN, Ghazanfar S, Guibentif C, Paulissen C, Baudelet E, Plaindoux E, Achouri Y, Calonne E, Dubois C, Mansfield W, Zaffran S, Marioni JC, Fuks F, Gottgens B, Lescroart F, Blanpain C. Nat Cell Biol. 2022 Jul;24(7):1114-1128. doi: 10.1038/s41556-022-00947-3. Epub 2022 Jul 11. PubMed
Template-independent genome editing in the Pcdh15(av-3j) mouse, a model of human DFNB23 nonsyndromic deafness. Liu L, Zou L, Li K, Hou H, Hu Q, Liu S, Li J, Song C, Chen J, Wang S, Wang Y, Li C, Du H, Li JL, Chen F, Xu Z, Sun W, Sun Q, Xiong W. Cell Rep. 2022 Jul 12;40(2):111061. doi: 10.1016/j.celrep.2022.111061. PubMed
seRNA PAM controls skeletal muscle satellite cell proliferation and aging through trans regulation of Timp2 expression synergistically with Ddx5. So KKH, Huang Y, Zhang S, Qiao Y, He L, Li Y, Chen X, Sham MH, Sun H, Wang H. Aging Cell. 2022 Aug;21(8):e13673. doi: 10.1111/acel.13673. Epub 2022 Jul 18. PubMed
Reactivity-dependent profiling of RNA 5-methylcytidine dioxygenases. Arguello AE, Li A, Sun X, Eggert TW, Mairhofer E, Kleiner RE. Nat Commun. 2022 Jul 19;13(1):4176. doi: 10.1038/s41467-022-31876-2. PubMed
A Method for In Situ Reverse Genetic Analysis of Proteins Involved mtDNA Replication. Kozhukhar N, Spadafora D, Rodriguez YAR, Alexeyev MF. Cells. 2022 Jul 11;11(14). pii: cells11142168. doi: 10.3390/cells11142168. PubMed Associated Plasmids pMA3735 pMA4854 pMA5096 pMA3998rc pMA3442 More ...
Establishment of CRISPR/Cas9 Genome-Editing System Based on Dual sgRNAs in Flammulina filiformis. Liu X, Dong J, Liao J, Tian L, Qiu H, Wu T, Ge F, Zhu J, Shi L, Jiang A, Yu H, Zhao M, Ren A. J Fungi (Basel). 2022 Jun 30;8(7):693. doi: 10.3390/jof8070693. PubMed
Nanodevices for the Efficient Codelivery of CRISPR-Cas9 Editing Machinery and an Entrapped Cargo: A Proposal for Dual Anti-Inflammatory Therapy. Garcia-Fernandez A, Vivo-Llorca G, Sancho M, Garcia-Jareno AB, Ramirez-Jimenez L, Barber-Cano E, Murguia JR, Orzaez M, Sancenon F, Martinez-Manez R. Pharmaceutics. 2022 Jul 19;14(7):1495. doi: 10.3390/pharmaceutics14071495. PubMed
Cholesterol Biosynthesis Modulates CSFV Replication. Zou X, Lin F, Yang Y, Chen J, Zhang H, Li L, Ouyang H, Pang D, Tang X. Viruses. 2022 Jun 30;14(7):1450. doi: 10.3390/v14071450. PubMed
Regulation of mitochondrial proteostasis by the proton gradient. Patron M, Tarasenko D, Nolte H, Kroczek L, Ghosh M, Ohba Y, Lasarzewski Y, Ahmadi ZA, Cabrera-Orefice A, Eyiama A, Kellermann T, Rugarli EI, Brandt U, Meinecke M, Langer T. EMBO J. 2022 Aug 16;41(16):e110476. doi: 10.15252/embj.2021110476. Epub 2022 Aug 1. PubMed
Nesprin-1 LINC complexes recruit microtubule cytoskeleton proteins and drive pathology in Lmna-mutant striated muscle. Leong EL, Khaing NT, Cadot B, Hong WL, Kozlov S, Werner H, Wong ESM, Stewart CL, Burke B, Lee YL. Hum Mol Genet. 2023 Jan 6;32(2):177-191. doi: 10.1093/hmg/ddac179. PubMed
Regulatory T Cell Depletion Using a CRISPR Fc-Optimized CD25 Antibody. van Elsas MJ, van der Schoot JMS, Bartels A, Steuten K, van Dalen D, Wijfjes Z, Figdor CG, van Hall T, van der Burg SH, Verdoes M, Scheeren FA. Int J Mol Sci. 2022 Aug 5;23(15):8707. doi: 10.3390/ijms23158707. PubMed
Modeling PRPF31 retinitis pigmentosa using retinal pigment epithelium and organoids combined with gene augmentation rescue. Rodrigues A, Slembrouck-Brec A, Nanteau C, Terray A, Tymoshenko Y, Zagar Y, Reichman S, Xi Z, Sahel JA, Fouquet S, Orieux G, Nandrot EF, Byrne LC, Audo I, Roger JE, Goureau O. NPJ Regen Med. 2022 Aug 16;7(1):39. doi: 10.1038/s41536-022-00235-6. PubMed
Alpha synuclein determines ferroptosis sensitivity in dopaminergic neurons via modulation of ether-phospholipid membrane composition. Mahoney-Sanchez L, Bouchaoui H, Boussaad I, Jonneaux A, Timmerman K, Berdeaux O, Ayton S, Kruger R, Duce JA, Devos D, Devedjian JC. Cell Rep. 2022 Aug 23;40(8):111231. doi: 10.1016/j.celrep.2022.111231. PubMed
Generation of corrected hiPSC clones from a Cornelia de Lange Syndrome (CdLS) patient through CRISPR-Cas-based technology. Umbach A, Maule G, Kheir E, Cutarelli A, Foglia M, Guarrera L, Fava LL, Conti L, Garattini E, Terao M, Cereseto A. Stem Cell Res Ther. 2022 Sep 2;13(1):440. doi: 10.1186/s13287-022-03135-0. PubMed
CAPG Is Required for Ebola Virus Infection by Controlling Virus Egress from Infected Cells. Mori H, Connell JP, Donahue CJ, Boytz R, Nguyen YTK, Leung DW, LaCount DJ, Davey RA. Viruses. 2022 Aug 28;14(9):1903. doi: 10.3390/v14091903. PubMed
A Clathrin light chain A reporter mouse for in vivo imaging of endocytosis. Grimm E, van der Hoeven F, Sardella D, Willig KI, Engel U, Veits N, Engel R, Cavalcanti-Adam EA, Bestvater F, Bordoni L, Jennemann R, Schonig K, Schiessl IM, Sandhoff R. PLoS One. 2022 Sep 23;17(9):e0273660. doi: 10.1371/journal.pone.0273660. eCollection 2022. PubMed
Monitoring autochthonous lung tumors induced by somatic CRISPR gene editing in mice using a secreted luciferase. Merle N, Elmshauser S, Strassheimer F, Wanzel M, Konig AM, Funk J, Neumann M, Kochhan K, Helmprobst F, Pagenstecher A, Nist A, Mernberger M, Schneider A, Braun T, Borggrefe T, Savai R, Timofeev O, Stiewe T. Mol Cancer. 2022 Oct 3;21(1):191. doi: 10.1186/s12943-022-01661-2. PubMed Associated Plasmids pShuttle pShuttle.Cre pShuttle.CC9 pShuttle-PR.CC9 pShuttle-PR.Cre More ...
A human iPSC-array-based GWAS identifies a virus susceptibility locus in the NDUFA4 gene and functional variants. Han Y, Tan L, Zhou T, Yang L, Carrau L, Lacko LA, Saeed M, Zhu J, Zhao Z, Nilsson-Payant BE, Lira Neto FT, Cahir C, Giani AM, Chai JC, Li Y, Dong X, Moroziewicz D, Paull D, Zhang T, Koo S, Tan C, Danziger R, Ba Q, Feng L, Chen Z, Zhong A, Wise GJ, Xiang JZ, Wang H, Schwartz RE, tenOever BR, Noggle SA, Rice CM, Qi Q, Evans T, Chen S. Cell Stem Cell. 2022 Oct 6;29(10):1475-1490.e6. doi: 10.1016/j.stem.2022.09.008. PubMed
A synthetic transcription platform for programmable gene expression in mammalian cells. Chen WCW, Gaidukov L, Lai Y, Wu MR, Cao J, Gutbrod MJ, Choi GCG, Utomo RP, Chen YC, Wroblewska L, Kellis M, Zhang L, Weiss R, Lu TK. Nat Commun. 2022 Oct 18;13(1):6167. doi: 10.1038/s41467-022-33287-9. PubMed
Development of an in vivo cleavable donor plasmid for targeted transgene integration by CRISPR-Cas9 and CRISPR-Cas12a. Ishibashi R, Maki R, Kitano S, Miyachi H, Toyoshima F. Sci Rep. 2022 Oct 22;12(1):17775. doi: 10.1038/s41598-022-22639-6. PubMed
A cell-based chemical-genetic screen for amino acid stress response inhibitors reveals torins reverse stress kinase GCN2 signaling. Bruggenthies JB, Fiore A, Russier M, Bitsina C, Brotzmann J, Kordes S, Menninger S, Wolf A, Conti E, Eickhoff JE, Murray PJ. J Biol Chem. 2022 Dec;298(12):102629. doi: 10.1016/j.jbc.2022.102629. Epub 2022 Oct 20. PubMed
Transient inhibition of p53 enhances prime editing and cytosine base-editing efficiencies in human pluripotent stem cells. Li M, Zhong A, Wu Y, Sidharta M, Beaury M, Zhao X, Studer L, Zhou T. Nat Commun. 2022 Oct 27;13(1):6354. doi: 10.1038/s41467-022-34045-7. PubMed Associated Plasmids pCMV-PE2-p53DD pEF_PE2 pegRNA-GBA-10 pegRNA_LRRK2-3 pegRNA_LMNA-1 More ...
Adult human kidney organoids originate from CD24(+) cells and represent an advanced model for adult polycystic kidney disease. Xu Y, Kuppe C, Perales-Paton J, Hayat S, Kranz J, Abdallah AT, Nagai J, Li Z, Peisker F, Saritas T, Halder M, Menzel S, Hoeft K, Kenter A, Kim H, van Roeyen CRC, Lehrke M, Moellmann J, Speer T, Buhl EM, Hoogenboezem R, Boor P, Jansen J, Knopp C, Kurth I, Smeets B, Bindels E, Reinders MEJ, Baan C, Gribnau J, Hoorn EJ, Steffens J, Huber TB, Costa I, Floege J, Schneider RK, Saez-Rodriguez J, Freedman BS, Kramann R. Nat Genet. 2022 Nov;54(11):1690-1701. doi: 10.1038/s41588-022-01202-z. Epub 2022 Oct 27. PubMed
Erratic and blood vessel-guided migration of astrocyte progenitors in the cerebral cortex. Tabata H, Sasaki M, Agetsuma M, Sano H, Hirota Y, Miyajima M, Hayashi K, Honda T, Nishikawa M, Inaguma Y, Ito H, Takebayashi H, Ema M, Ikenaka K, Nabekura J, Nagata KI, Nakajima K. Nat Commun. 2022 Nov 2;13(1):6571. doi: 10.1038/s41467-022-34184-x. PubMed
MARCH5 regulates mitotic apoptosis through MCL1-dependent and independent mechanisms. Wang Y, Poon RYC. Cell Death Differ. 2023 Mar;30(3):753-765. doi: 10.1038/s41418-022-01080-2. Epub 2022 Nov 3. PubMed
The balance between NANOG and SOX17 mediated by TET proteins regulates specification of human primordial germ cell fate. Li Z, Fang F, Long Y, Zhao Q, Wang X, Ye Z, Meng T, Gu X, Xiang W, Xiong C, Li H. Cell Biosci. 2022 Nov 4;12(1):181. doi: 10.1186/s13578-022-00917-0. PubMed
Generation of APN-chimeric gene-edited pigs by CRISPR/Cas9-mediated knock-in strategy. Liu Z, Zhang M, Huang P, Ji Z, Qi C, Jiao S, Zhao D, Jiang Y, Chen X, Lv D, Pang D, Zhang X, Feng L, Xie Z, Ouyang H. Gene. 2023 Jan 30;851:147007. doi: 10.1016/j.gene.2022.147007. Epub 2022 Nov 2. PubMed
Lactate facilitates classical swine fever virus replication by enhancing cholesterol biosynthesis. Zou X, Yang Y, Lin F, Chen J, Zhang H, Li L, Ouyang H, Pang D, Ren L, Tang X. iScience. 2022 Oct 13;25(11):105353. doi: 10.1016/j.isci.2022.105353. eCollection 2022 Nov 18. PubMed
Transcription factor antagonism regulates heterogeneity in embryonic stem cell states. Hu S, Metcalf E, Mahat DB, Chan L, Sohal N, Chakraborty M, Hamilton M, Singh A, Singh A, Lees JA, Sharp PA, Garg S. Mol Cell. 2022 Dec 1;82(23):4410-4427.e12. doi: 10.1016/j.molcel.2022.10.022. Epub 2022 Nov 9. PubMed
MYBPC3 deficiency in cardiac fibroblasts drives their activation and contributes to fibrosis. Zou X, Ouyang H, Lin F, Zhang H, Yang Y, Pang D, Han R, Tang X. Cell Death Dis. 2022 Nov 10;13(11):948. doi: 10.1038/s41419-022-05403-6. PubMed
Correlation between the expression of LAT1 in cancer cells and the potential efficacy of boron neutron capture therapy. Watanabe T, Sanada Y, Hattori Y, Suzuki M. J Radiat Res. 2023 Jan 20;64(1):91-98. doi: 10.1093/jrr/rrac077. PubMed
A general approach to identify cell-permeable and synthetic anti-CRISPR small molecules. Lim D, Zhou Q, Cox KJ, Law BK, Lee M, Kokkonda P, Sreekanth V, Pergu R, Chaudhary SK, Gangopadhyay SA, Maji B, Lai S, Amako Y, Thompson DB, Subramanian HKK, Mesleh MF, Dancik V, Clemons PA, Wagner BK, Woo CM, Church GM, Choudhary A. Nat Cell Biol. 2022 Dec;24(12):1766-1775. doi: 10.1038/s41556-022-01005-8. Epub 2022 Nov 17. PubMed
Osteoblast/osteocyte-derived interleukin-11 regulates osteogenesis and systemic adipogenesis. Dong B, Hiasa M, Higa Y, Ohnishi Y, Endo I, Kondo T, Takashi Y, Tsoumpra M, Kainuma R, Sawatsubashi S, Kiyonari H, Shioi G, Sakaue H, Nakashima T, Kato S, Abe M, Fukumoto S, Matsumoto T. Nat Commun. 2022 Nov 23;13(1):7194. doi: 10.1038/s41467-022-34869-3. PubMed
Identification and Analysis of Small Molecule Inhibitors of CRISPR-Cas9 in Human Cells. Yang Y, Li D, Wan F, Chen B, Wu G, Li F, Ren Y, Liang P, Wan J, Songyang Z. Cells. 2022 Nov 11;11(22):3574. doi: 10.3390/cells11223574. PubMed
Low-dose AAV-CRISPR-mediated liver-specific knock-in restored hemostasis in neonatal hemophilia B mice with subtle antibody response. He X, Zhang Z, Xue J, Wang Y, Zhang S, Wei J, Zhang C, Wang J, Urip BA, Ngan CC, Sun J, Li Y, Lu Z, Zhao H, Pei D, Li CK, Feng B. Nat Commun. 2022 Nov 25;13(1):7275. doi: 10.1038/s41467-022-34898-y. PubMed
Distinct changes in endosomal composition promote NLRP3 inflammasome activation. Zhang Z, Venditti R, Ran L, Liu Z, Vivot K, Schurmann A, Bonifacino JS, De Matteis MA, Ricci R. Nat Immunol. 2023 Jan;24(1):30-41. doi: 10.1038/s41590-022-01355-3. Epub 2022 Nov 28. PubMed
Cellular reprogramming with ATOH1, GFI1, and POU4F3 implicate epigenetic changes and cell-cell signaling as obstacles to hair cell regeneration in mature mammals. Iyer AA, Hosamani I, Nguyen JD, Cai T, Singh S, McGovern MM, Beyer L, Zhang H, Jen HI, Yousaf R, Birol O, Sun JJ, Ray RS, Raphael Y, Segil N, Groves AK. Elife. 2022 Nov 29;11:e79712. doi: 10.7554/eLife.79712. PubMed
Self-cleaving guide RNAs enable pharmacological selection of precise gene editing events in vivo. Tiyaboonchai A, Vonada A, Posey J, Pelz C, Wakefield L, Grompe M. Nat Commun. 2022 Nov 30;13(1):7391. doi: 10.1038/s41467-022-35097-5. PubMed
Substitution of PINK1 Gly411 modulates substrate receptivity and turnover. Fiesel FC, Fricova D, Hayes CS, Coban MA, Hudec R, Bredenberg JM, Broadway BJ, Markham BN, Yan T, Boneski PK, Fiorino G, Watzlawik JO, Hou X, McCarty AM, Lewis-Tuffin LJ, Zhong J, Madden BJ, Ordureau A, An H, Puschmann A, Wszolek ZK, Ross OA, Harper JW, Caulfield TR, Springer W. Autophagy. 2023 Jun;19(6):1711-1732. doi: 10.1080/15548627.2022.2151294. Epub 2022 Dec 5. PubMed
Mouse placenta fetal macrophages arise from endothelial cells outside the placenta. Chen X, Tang AT, Tober J, Yang J, Leu NA, Sterling S, Chen M, Yang Y, Mericko-Ishizuka P, Speck NA, Kahn ML. Dev Cell. 2022 Dec 5;57(23):2652-2660.e3. doi: 10.1016/j.devcel.2022.11.003. PubMed
Characterization of mutant versions of the R-RAS2/TC21 GTPase found in tumors. Clavain L, Fernandez-Pisonero I, Movilla N, Lorenzo-Martin LF, Nieto B, Abad A, Garcia-Navas R, Llorente-Gonzalez C, Sanchez-Martin M, Vicente-Manzanares M, Santos E, Alarcon B, Garcia-Aznar JM, Dosil M, Bustelo XR. Oncogene. 2023 Jan;42(5):389-405. doi: 10.1038/s41388-022-02563-9. Epub 2022 Dec 7. PubMed
Establishment of isogenic induced pluripotent stem cells with or without pathogenic mutation for understanding the pathogenesis of myeloproliferative neoplasms. Liu C, Imai M, Edahiro Y, Mano S, Takei H, Nudejima M, Kurose A, Morishita S, Ando M, Tsuneda S, Araki M, Komatsu N. Exp Hematol. 2023 Feb;118:12-20. doi: 10.1016/j.exphem.2022.11.005. Epub 2022 Nov 26. PubMed
The UFM1 system regulates ER-phagy through the ufmylation of CYB5R3. Ishimura R, El-Gowily AH, Noshiro D, Komatsu-Hirota S, Ono Y, Shindo M, Hatta T, Abe M, Uemura T, Lee-Okada HC, Mohamed TM, Yokomizo T, Ueno T, Sakimura K, Natsume T, Sorimachi H, Inada T, Waguri S, Noda NN, Komatsu M. Nat Commun. 2022 Dec 21;13(1):7857. doi: 10.1038/s41467-022-35501-0. PubMed Associated Plasmids pHis-3xFLAG-CMV14-CYB5R3 pHis-3xFLAG-CMV14-CYB5R3 K214R pIRESpuroN3xFLAG-UFSP2 pIRESpuroN3xFLAG-UFSP2 C302A pIRESpuroN3xFLAG-His-CDK5RAP3 More ...
In vitro genome editing activity of Cas9 in somatic cells after random and transposon-based genomic Cas9 integration. Sollner JH, Sake HJ, Frenzel A, Lechler R, Herrmann D, Fuchs W, Petersen B. PLoS One. 2022 Dec 30;17(12):e0279123. doi: 10.1371/journal.pone.0279123. eCollection 2022. PubMed
Dynamics of endogenous PARP1 and PARP2 during DNA damage revealed by live-cell single-molecule imaging. Mahadevan J, Jha A, Rudolph J, Bowerman S, Narducci D, Hansen AS, Luger K. iScience. 2022 Dec 9;26(1):105779. doi: 10.1016/j.isci.2022.105779. eCollection 2023 Jan 20. PubMed
ZNF212 promotes genomic integrity through direct interaction with TRAIP. Chung HJ, Lee JR, Kim TM, Kim S, Park K, Kim MJ, Jung E, Kim S, Lee EA, Ra JS, Hwang S, Lee JY, Scharer OD, Kim Y, Myung K, Kim H. Nucleic Acids Res. 2023 Jan 25;51(2):631-649. doi: 10.1093/nar/gkac1226. PubMed
ERAP2 supports TCR recognition of three immunotherapy targeted tumor epitopes. Schmidt K, Leisegang M, Kloetzel PM. Mol Immunol. 2023 Feb;154:61-68. doi: 10.1016/j.molimm.2022.12.010. Epub 2023 Jan 4. PubMed
The human pre-replication complex is an open complex. Li J, Dong J, Wang W, Yu D, Fan X, Hui YC, Lee CSK, Lam WH, Alary N, Yang Y, Zhang Y, Zhao Q, Chen CL, Tye BK, Dang S, Zhai Y. Cell. 2023 Jan 5;186(1):98-111.e21. doi: 10.1016/j.cell.2022.12.008. PubMed
RIG-I immunotherapy overcomes radioresistance in p53-positive malignant melanoma. Lambing S, Tan YP, Vasileiadou P, Holdenrieder S, Muller P, Hagen C, Garbe S, Behrendt R, Schlee M, van den Boorn JG, Bartok E, Renn M, Hartmann G. J Mol Cell Biol. 2023 Jun 1;15(1):mjad001. doi: 10.1093/jmcb/mjad001. PubMed
Loss of RREB1 in pancreatic beta cells reduces cellular insulin content and affects endocrine cell gene expression. Mattis KK, Krentz NAJ, Metzendorf C, Abaitua F, Spigelman AF, Sun H, Ikle JM, Thaman S, Rottner AK, Bautista A, Mazzaferro E, Perez-Alcantara M, Manning Fox JE, Torres JM, Wesolowska-Andersen A, Yu GZ, Mahajan A, Larsson A, MacDonald PE, Davies B, den Hoed M, Gloyn AL. Diabetologia. 2023 Apr;66(4):674-694. doi: 10.1007/s00125-022-05856-6. Epub 2023 Jan 12. PubMed
HER2-driven breast cancer suppression by the JNK signaling pathway. Itah Z, Chaudhry S, Raju Ponny S, Aydemir O, Lee A, Cavanagh-Kyros J, Tournier C, Muller WJ, Davis RJ. Proc Natl Acad Sci U S A. 2023 Jan 24;120(4):e2218373120. doi: 10.1073/pnas.2218373120. Epub 2023 Jan 19. PubMed
Construction of a Set of Novel Transposon Vectors for Efficient Silencing of Protein and lncRNA Genes via CRISPR Interference. Czarnek M, Kochan J, Wawro M, Myrczek R, Bereta J. Mol Biotechnol. 2023 Jan 28. doi: 10.1007/s12033-023-00675-5. PubMed Associated Plasmids pSBbi-Bla-dCas9-KRAB-MeCP2_hU6-SapI pSBbi-Bla-dCas9-KRAB-MeCP2_hU6-A10 pSBbi-Hyg-dCas9-KRAB-MeCP2_hU6-SapI pSBbi-Hyg-dCas9-KRAB-MeCP2_hU6-A10 pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-SapI More ...
Impaired migration and premature differentiation underlie the neurological phenotype associated with PCDH12 loss of function. Rakotomamonjy J, Rylaarsdam L, Fares-Taie L, McDermott S, Davies D, Yang G, Fagbemi F, Epstein M, Guemez-Gamboa A. bioRxiv. 2023 Jan 5:2023.01.05.522934. doi: 10.1101/2023.01.05.522934. Preprint. PubMed
Cyclin A-CDK1 suppresses the expression of the CDK1 activator CDC25A to safeguard timely mitotic entry. Ng LY, Ma HT, Poon RYC. J Biol Chem. 2023 Mar;299(3):102957. doi: 10.1016/j.jbc.2023.102957. Epub 2023 Jan 28. PubMed
RIF1 suppresses the formation of single-stranded ultrafine anaphase bridges via protein phosphatase 1. Kong N, Liu Z, Chan YW. Cell Rep. 2023 Jan 30;42(2):112032. doi: 10.1016/j.celrep.2023.112032. PubMed
The ADAM17 sheddase complex regulator iTAP/Frmd8 modulates inflammation and tumor growth. Badenes M, Burbridge E, Oikonomidi I, Amin A, de Carvalho E, Kosack L, Mariano C, Domingos P, Faisca P, Adrain C. Life Sci Alliance. 2023 Jan 31;6(4):e202201644. doi: 10.26508/lsa.202201644. Print 2023 Apr. PubMed
MDM2 antagonists promote CRISPR/Cas9-mediated precise genome editing in sheep primary cells. Li Y, Lian D, Wang J, Zhao Y, Li Y, Liu G, Wu S, Deng S, Du X, Lian Z. Mol Ther Nucleic Acids. 2023 Jan 2;31:309-323. doi: 10.1016/j.omtn.2022.12.020. eCollection 2023 Mar 14. PubMed
Increased angiotensin II coupled with decreased Adra1a expression enhances cardiac hypertrophy in pregnancy-associated hypertensive mice. Kim JD, Kwon C, Nakamura K, Muromachi N, Mori H, Muroi SI, Yamada Y, Saito H, Nakagawa Y, Fukamizu A. J Biol Chem. 2023 Mar;299(3):102964. doi: 10.1016/j.jbc.2023.102964. Epub 2023 Feb 2. PubMed
Mitotic DNA synthesis in response to replication stress requires the sequential action of DNA polymerases zeta and delta in human cells. Wu W, Barwacz SA, Bhowmick R, Lundgaard K, Goncalves Dinis MM, Clausen M, Kanemaki MT, Liu Y. Nat Commun. 2023 Feb 9;14(1):706. doi: 10.1038/s41467-023-35992-5. PubMed
A genome-wide CRISPR-Cas9 knockout screen identifies FSP1 as the warfarin-resistant vitamin K reductase. Jin DY, Chen X, Liu Y, Williams CM, Pedersen LC, Stafford DW, Tie JK. Nat Commun. 2023 Feb 14;14(1):828. doi: 10.1038/s41467-023-36446-8. PubMed
The Glucocorticoid Receptor is Required for Efficient Aldosterone-Induced Transcription by the Mineralocorticoid Receptor. Johnson TA, Fettweis G, Wagh K, Almeida-Prieto B, Krishnamurthy M, Upadhyaya A, Hager GL, Alvarez de la Rosa D. bioRxiv. 2023 Jun 17:2023.01.26.525745. doi: 10.1101/2023.01.26.525745. Preprint. PubMed
Phase separation of an actin nucleator by junctional microtubules regulates epithelial function. Tsukita K, Kitamata M, Kashihara H, Yano T, Fujiwara I, Day TF, Katsuno T, Kim J, Takenaga F, Tanaka H, Park S, Miyata M, Watanabe H, Kondoh G, Takahashi R, Tamura A, Tsukita S. Sci Adv. 2023 Feb 15;9(7):eadf6358. doi: 10.1126/sciadv.adf6358. Epub 2023 Feb 15. PubMed
Directed differentiation of human iPSCs to functional ovarian granulosa-like cells via transcription factor overexpression. Pierson Smela MD, Kramme CC, Fortuna PRJ, Adams JL, Su R, Dong E, Kobayashi M, Brixi G, Kavirayuni VS, Tysinger E, Kohman RE, Shioda T, Chatterjee P, Church GM. Elife. 2023 Feb 21;12:e83291. doi: 10.7554/eLife.83291. PubMed Associated Plasmids pHDR-FOXL2-T2A-tdTomato-PuroTK pX330-FOXL2-cterm pPB-cT3G-cERP2-TCF21 pPB-cT3G-cERP2-JUN pPB-cT3G-cERP2-GATA4 More ...
Induction of primordial germ cell-like cells from common marmoset embryonic stem cells by inhibition of WNT and retinoic acid signaling. Shono M, Kishimoto K, Hikabe O, Hayashi M, Semi K, Takashima Y, Sasaki E, Kato K, Hayashi K. Sci Rep. 2023 Feb 23;13(1):3186. doi: 10.1038/s41598-023-29850-z. PubMed
Generation of human cerebral organoids with a structured outer subventricular zone. Walsh R, Giacomelli E, Ciceri G, Rittenhouse C, Galimberti M, Wu Y, Muller J, Vezzoli E, Jungverdorben J, Zhou T, Barker RA, Cattaneo E, Studer L, Baggiolini A. bioRxiv. 2023 Feb 17:2023.02.17.528906. doi: 10.1101/2023.02.17.528906. Preprint. PubMed
Increased On-Target Rate and Risk of Concatemerization after CRISPR-Enhanced Targeting in ES Cells. Erbs V, Lorentz R, Eisenman B, Schaeffer L, Luppi L, Lindner L, Herault Y, Pavlovic G, Wattenhofer-Donze M, Birling MC. Genes (Basel). 2023 Feb 3;14(2):401. doi: 10.3390/genes14020401. PubMed
Transcription of MERVL retrotransposons is required for preimplantation embryo development. Sakashita A, Kitano T, Ishizu H, Guo Y, Masuda H, Ariura M, Murano K, Siomi H. Nat Genet. 2023 Mar;55(3):484-495. doi: 10.1038/s41588-023-01324-y. Epub 2023 Mar 2. PubMed
NUDT22 promotes cancer growth through pyrimidine salvage. Walter M, Mayr F, Hanna BMF, Cookson V, Mortusewicz O, Helleday T, Herr P. Oncogene. 2023 Apr;42(16):1282-1293. doi: 10.1038/s41388-023-02643-4. Epub 2023 Mar 4. PubMed
EWSR1 prevents the induction of aneuploidy through direct regulation of Aurora B. Kim H, Park H, Schulz ET, Azuma Y, Azuma M. Front Cell Dev Biol. 2023 Feb 15;11:987153. doi: 10.3389/fcell.2023.987153. eCollection 2023. PubMed
An optimized SpCas9 high-fidelity variant for direct protein delivery. Pedrazzoli E, Bianchi A, Umbach A, Amistadi S, Brusson M, Frati G, Ciciani M, Badowska KA, Arosio D, Miccio A, Cereseto A, Casini A. Mol Ther. 2023 Jul 5;31(7):2257-2265. doi: 10.1016/j.ymthe.2023.03.007. Epub 2023 Mar 10. PubMed
Phosphatidylinositol-3,4,5-trisphosphate interacts with alpha-synuclein and initiates its aggregation and formation of Parkinson's disease-related fibril polymorphism. Choong CJ, Aguirre C, Kakuda K, Beck G, Nakanishi H, Kimura Y, Shimma S, Nabekura K, Hideshima M, Doi J, Yamaguchi K, Nakajima K, Wadayama T, Hayakawa H, Baba K, Ogawa K, Takeuchi T, Badawy SMM, Murayama S, Nagano S, Goto Y, Miyanoiri Y, Nagai Y, Mochizuki H, Ikenaka K. Acta Neuropathol. 2023 May;145(5):573-595. doi: 10.1007/s00401-023-02555-3. Epub 2023 Mar 20. PubMed
A gain-of-function mutation in microRNA 142 is sufficient to cause the development of T-cell leukemia in mice. Kawano S, Araki K, Bai J, Furukawa I, Tateishi K, Yoshinobu K, Usuki S, Nimmo RA, Kaname T, Yoshihara M, Takahashi S, Sashida G, Araki M. Cancer Sci. 2023 Jul;114(7):2821-2834. doi: 10.1111/cas.15794. Epub 2023 Apr 3. PubMed
Multiplexed genome engineering for porcine fetal fibroblasts with gRNA-tRNA arrays based on CRISPR/Cas9. Guo X, Geng L, Jiang C, Yao W, Jin J, Liu Z, Mu Y. Anim Biotechnol. 2023 Mar 22:1-10. doi: 10.1080/10495398.2023.2187402. PubMed
Endogenous bioluminescent reporters reveal a sustained increase in utrophin gene expression upon EZH2 and ERK1/2 inhibition. Gleneadie HJ, Fernandez-Ruiz B, Sardini A, Van de Pette M, Dimond A, Prinjha RK, McGinty J, French PMW, Bagci H, Merkenschlager M, Fisher AG. Commun Biol. 2023 Mar 25;6(1):318. doi: 10.1038/s42003-023-04666-9. PubMed
Myeloid-associated differentiation marker is an essential host factor for human parechovirus PeV-A3 entry. Watanabe K, Oka T, Takagi H, Anisimov S, Yamashita SI, Katsuragi Y, Takahashi M, Higuchi M, Kanki T, Saitoh A, Fujii M. Nat Commun. 2023 Mar 31;14(1):1817. doi: 10.1038/s41467-023-37399-8. PubMed
Mapping the SLP76 interactome in T cells lacking each of the GRB2-family adaptors reveals molecular plasticity of the TCR signaling pathway. Ruminski K, Celis-Gutierrez J, Jarmuzynski N, Maturin E, Audebert S, Malissen M, Camoin L, Voisinne G, Malissen B, Roncagalli R. Front Immunol. 2023 Mar 15;14:1139123. doi: 10.3389/fimmu.2023.1139123. eCollection 2023. PubMed
Characterizing the differential distribution and targets of Sumo1 and Sumo2 in the mouse brain. Suk TR, Nguyen TT, Fisk ZA, Mitkovski M, Geertsma HM, Parmasad JA, Heer MM, Callaghan SM, Benseler F, Brose N, Tirard M, Rousseaux MWC. iScience. 2023 Mar 9;26(4):106350. doi: 10.1016/j.isci.2023.106350. eCollection 2023 Apr 21. PubMed
Retinitis pigmentosa-associated mutations in mouse Prpf8 cause misexpression of circRNAs and degeneration of cerebellar granule cells. Krausova M, Kreplova M, Banik P, Cvackova Z, Kubovciak J, Modrak M, Zudova D, Lindovsky J, Kubik-Zahorodna A, Palkova M, Kolar M, Prochazka J, Sedlacek R, Stanek D. Life Sci Alliance. 2023 Apr 5;6(6):e202201855. doi: 10.26508/lsa.202201855. Print 2023 Jun. PubMed
RBBP4 is an epigenetic barrier for the induced transition of pluripotent stem cells into totipotent 2C-like cells. Ping W, Sheng Y, Hu G, Zhong H, Li Y, Liu Y, Luo W, Yan C, Wen Y, Wang X, Li Q, Guo R, Zhang J, Liu A, Pan G, Yao H. Nucleic Acids Res. 2023 Jun 23;51(11):5414-5431. doi: 10.1093/nar/gkad219. PubMed
The Tip60/Ep400 chromatin remodeling complex impacts basic cellular functions in cranial neural crest-derived tissue during early orofacial development. Gehlen-Breitbach S, Schmid T, Frob F, Rodrian G, Weider M, Wegner M, Golz L. Int J Oral Sci. 2023 Apr 6;15(1):16. doi: 10.1038/s41368-023-00222-7. PubMed
Amniogenesis in Human Amniotic Sac Embryoids after Exposures to Organophosphate Flame Retardants. Xu C, Zhang C, Liu Y, Ma H, Wu F, Jia Y, Hu J. Environ Health Perspect. 2023 Apr;131(4):47007. doi: 10.1289/EHP11958. Epub 2023 Apr 7. PubMed
FLASH-RT does not affect chromosome translocations and junction structures beyond that of CONV-RT dose-rates. Barghouth PG, Melemenidis S, Montay-Gruel P, Ollivier J, Viswanathan V, Jorge PG, Soto LA, Lau BC, Sadeghi C, Edlabadkar A, Manjappa R, Wang J, Bouteiller ML, Surucu M, Yu A, Bush K, Skinner L, Maxim PG, Loo BW, Limoli CL, Vozenin MC, Frock RL. bioRxiv. 2023 Mar 27:2023.03.27.534408. doi: 10.1101/2023.03.27.534408. Preprint. PubMed
High TXNIP expression accelerates the migration and invasion of the GDM placenta trophoblast. Sa R, Ma J, Yang J, Li DF, Du J, Jia JC, Li ZY, Huang N, A L, Sha R, Nai G, Hexig B, Meng JQ, Yu L. BMC Pregnancy Childbirth. 2023 Apr 10;23(1):235. doi: 10.1186/s12884-023-05524-6. PubMed
Molecular basis of Climp63-mediated ER lumen spacing. Xu L, Xiang Y, Hu J. J Cell Sci. 2023 May 1;136(9):jcs260976. doi: 10.1242/jcs.260976. Epub 2023 Apr 28. PubMed
Synergistic effects of combined immunotherapy strategies in a model of multifocal hepatocellular carcinoma. Ochoa MC, Sanchez-Gregorio S, de Andrea CE, Garasa S, Alvarez M, Olivera I, Glez-Vaz J, Luri-Rey C, Etxeberria I, Cirella A, Azpilikueta A, Berraondo P, Argemi J, Sangro B, Teijeira A, Melero I. Cell Rep Med. 2023 Apr 18;4(4):101009. doi: 10.1016/j.xcrm.2023.101009. Epub 2023 Apr 10. PubMed
An A-kinase anchoring protein (ACBD3) coordinates traffic-induced PKA activation at the Golgi. Jia J, Tang S, Yue X, Jing S, Zhu L, Tan C, Gao J, Du Y, Lee I, Qian Y. J Biol Chem. 2023 May;299(5):104696. doi: 10.1016/j.jbc.2023.104696. Epub 2023 Apr 10. PubMed
A lncRNA from an inflammatory bowel disease risk locus maintains intestinal host-commensal homeostasis. Ma H, Hu T, Tao W, Tong J, Han Z, Herndler-Brandstetter D, Wei Z, Liu R, Zhou T, Liu Q, Xu X, Zhang K, Zhou R, Cho JH, Li HB, Huang H, Flavell RA, Zhu S. Cell Res. 2023 May;33(5):372-388. doi: 10.1038/s41422-023-00790-7. Epub 2023 Apr 13. PubMed
A monoclonal antibody raised against human EZH2 cross-reacts with the RNA-binding protein SAFB. Cherney RE, Mills CA, Herring LE, Braceros AK, Calabrese JM. bioRxiv. 2023 Apr 3:2023.04.03.535391. doi: 10.1101/2023.04.03.535391. Preprint. PubMed Associated Plasmids pX330_EZH2gRNA14 pX330_EZH2gRNA15 pX330_EZH2gRNA16 pX330_EZH2gRNA17
A Rapid, Functional sgRNA Screening Method for Generating Murine RET and NTRK1 Fusion Oncogenes. Schubert L, Le AT, Hinz TK, Navarro A, Nelson-Taylor SK, Nemenoff RA, Heasley LE, Doebele RC. bioRxiv. 2023 Apr 6:2023.04.06.535912. doi: 10.1101/2023.04.06.535912. Preprint. PubMed
The Majority of the Serine/Threonine Phosphorylation Sites in Bcl11b Protein Are Dispensable for the Differentiation of T Cells. Okuyama K, Nomura A, Nishino K, Tanaka H, Harly C, Chihara R, Harada Y, Muroi S, Kubo M, Kosako H, Taniuchi I. J Immunol. 2023 Jun 1;210(11):1728-1739. doi: 10.4049/jimmunol.2200101. PubMed
Vesicular Integral-Membrane Protein 36 Is Involved in the Selective Secretion of Fucosylated Proteins into Bile Duct-like Structures in HepG2 Cells. Muranaka M, Takamatsu S, Ouchida T, Kanazawa Y, Kondo J, Nakagawa T, Egashira Y, Fukagawa K, Gu J, Okamoto T, Kamada Y, Miyoshi E. Int J Mol Sci. 2023 Apr 11;24(8):7037. doi: 10.3390/ijms24087037. PubMed
The role of kinetochore dynein in checkpoint silencing is restricted to disassembly of the corona. Ide AH, DeLuca KF, Wiggan O, Markus SM, DeLuca JG. Mol Biol Cell. 2023 Jun 1;34(7):ar76. doi: 10.1091/mbc.E23-04-0130. Epub 2023 Apr 26. PubMed
Co-delivery of Cas9 mRNA and guide RNAs edits hepatitis B virus episomal and integration DNA in mouse and tree shrew models. Yi J, Lei X, Guo F, Chen Q, Chen X, Zhao K, Zhu C, Cheng X, Lin J, Yin H, Xia Y. Antiviral Res. 2023 Jul;215:105618. doi: 10.1016/j.antiviral.2023.105618. Epub 2023 May 2. PubMed
A tug of war between DCC and ROBO1 signaling during commissural axon guidance. Dailey-Krempel B, Martin AL, Jo HN, Junge HJ, Chen Z. Cell Rep. 2023 May 30;42(5):112455. doi: 10.1016/j.celrep.2023.112455. Epub 2023 May 5. PubMed
miR-145 Modulates Fatty Acid Metabolism by Targeting FOXO1 to Affect SERBP1 Activity in Bovine Mammary Epithelial Cells. Lyu M, Li F, Wang X, Xu K, Sun S. J Agric Food Chem. 2023 May 17;71(19):7440-7450. doi: 10.1021/acs.jafc.2c09001. Epub 2023 May 8. PubMed
Interaction of LARP4 to filamin A mechanosensing domain regulates cell migrations. Mao Z, Nakamura F. Front Cell Dev Biol. 2023 Apr 24;11:1152109. doi: 10.3389/fcell.2023.1152109. eCollection 2023. PubMed
Fendrr synergizes with Wnt signalling to regulate fibrosis related genes during lung development via its RNA:dsDNA triplex element. Ali T, Rogala S, Krause NM, Bains JK, Melissari MT, Wahrisch S, Schwalbe H, Herrmann BG, Grote P. Nucleic Acids Res. 2023 Jul 7;51(12):6227-6237. doi: 10.1093/nar/gkad395. PubMed
MAP4K3 inhibits Sirtuin-1 to repress the LKB1-AMPK pathway to promote amino acid-dependent activation of the mTORC1 complex. Branch MR, Hsu CL, Ohnishi K, Shen WC, Lee E, Meisenhelder J, Winborn B, Sopher BL, Taylor JP, Hunter T, La Spada AR. Life Sci Alliance. 2023 May 23;6(8):e202201525. doi: 10.26508/lsa.202201525. Print 2023 Aug. PubMed
Protocol for biallelic tagging of an endogenous gene using CRISPR-Cas9 in human cells. Kong N, Chan YW. STAR Protoc. 2023 May 29;4(2):102286. doi: 10.1016/j.xpro.2023.102286. PubMed
Nicotinamide N-methyltransferase sustains a core epigenetic program that promotes metastatic colonization in breast cancer. Couto JP, Vulin M, Jehanno C, Coissieux MM, Hamelin B, Schmidt A, Ivanek R, Sethi A, Brautigam K, Frei AL, Hager C, Manivannan M, Gomez-Miragaya J, Obradovic MM, Varga Z, Koelzer VH, Mertz KD, Bentires-Alj M. EMBO J. 2023 Jul 3;42(13):e112559. doi: 10.15252/embj.2022112559. Epub 2023 Jun 1. PubMed
MLL3 regulates the CDKN2A tumor suppressor locus in liver cancer. Zhu C, Soto-Feliciano YM, Morris JP, Huang CH, Koche RP, Ho YJ, Banito A, Chen CW, Shroff A, Tian S, Livshits G, Chen CC, Fennell M, Armstrong SA, Allis CD, Tschaharganeh DF, Lowe SW. Elife. 2023 Jun 1;12:e80854. doi: 10.7554/eLife.80854. PubMed
Five nucleotides found in RCTG motifs are essential for post-fertilization methylation imprinting of the H19 ICR in YAC transgenic mice. Matsuzaki H, Takahashi T, Kuramochi D, Hirakawa K, Tanimoto K. Nucleic Acids Res. 2023 Aug 11;51(14):7236-7253. doi: 10.1093/nar/gkad516. PubMed
Mitochondrial supercomplex assembly regulates metabolic features and glutamine dependency in mammalian cells. Zhang K, Chen L, Wang B, Chen D, Ye X, Han X, Fang Q, Yu C, Wu J, Guo S, Chen L, Shi Y, Wang L, Cheng H, Li H, Shen L, Zhao Q, Jin L, Lyu J, Fang H. Theranostics. 2023 May 21;13(10):3165-3187. doi: 10.7150/thno.78292. eCollection 2023. PubMed
Null and missense mutations of ERI1 cause a recessive phenotypic dichotomy in humans. Guo L, Salian S, Xue JY, Rath N, Rousseau J, Kim H, Ehresmann S, Moosa S, Nakagawa N, Kuroda H, Clayton-Smith J, Wang J, Wang Z, Banka S, Jackson A, Zhang YM, Wei ZJ, Huning I, Brunet T, Ohashi H, Thomas MF, Bupp C, Miyake N, Matsumoto N, Mendoza-Londono R, Costain G, Hahn G, Di Donato N, Yigit G, Yamada T, Nishimura G, Ansel KM, Wollnik B, Hrabe de Angelis M, Megarbane A, Rosenfeld JA, Heissmeyer V, Ikegawa S, Campeau PM. Am J Hum Genet. 2023 Jul 6;110(7):1068-1085. doi: 10.1016/j.ajhg.2023.06.001. Epub 2023 Jun 22. PubMed
Human artificial chromosome carrying 3p21.3-p22.2 region suppresses hTERT transcription in oral cancer cells. Ohira T, Yoshimura K, Kugoh H. Chromosome Res. 2023 Jun 24;31(3):17. doi: 10.1007/s10577-023-09726-8. PubMed
Method of Monitoring 26S Proteasome in Cells Revealed the Crucial Role of PSMA3 C-Terminus in 26S Integrity. Steinberger S, Adler J, Shaul Y. Biomolecules. 2023 Jun 15;13(6):992. doi: 10.3390/biom13060992. PubMed
A Cell-Based Optimised Approach for Rapid and Efficient Gene Editing of Human Pluripotent Stem Cells. Cuevas-Ocana S, Yang JY, Aushev M, Schlossmacher G, Bear CE, Hannan NRF, Perkins ND, Rossant J, Wong AP, Gray MA. Int J Mol Sci. 2023 Jun 17;24(12):10266. doi: 10.3390/ijms241210266. PubMed
How chromosomal translocations arise to cause cancer: Gene proximity, trans-splicing, and DNA end joining. Streb P, Kowarz E, Benz T, Reis J, Marschalek R. iScience. 2023 May 19;26(6):106900. doi: 10.1016/j.isci.2023.106900. eCollection 2023 Jun 16. PubMed
Cancer aneuploidies are shaped primarily by effects on tumour fitness. Shih J, Sarmashghi S, Zhakula-Kostadinova N, Zhang S, Georgis Y, Hoyt SH, Cuoco MS, Gao GF, Spurr LF, Berger AC, Ha G, Rendo V, Shen H, Meyerson M, Cherniack AD, Taylor AM, Beroukhim R. Nature. 2023 Jul;619(7971):793-800. doi: 10.1038/s41586-023-06266-3. Epub 2023 Jun 28. PubMed
Dissecting the roles of MBD2 isoforms and domains in regulating NuRD complex function during cellular differentiation. Schmolka N, Karemaker ID, Cardoso da Silva R, Recchia DC, Spegg V, Bhaskaran J, Teske M, de Wagenaar NP, Altmeyer M, Baubec T. Nat Commun. 2023 Jun 29;14(1):3848. doi: 10.1038/s41467-023-39551-w. PubMed
Functional glycoproteomics by integrated network assembly and partitioning. Griffin ME, Thompson JW, Xiao Y, Sweredoski MJ, Aksenfeld RB, Jensen EH, Koldobskaya Y, Schacht AL, Kim TD, Choudhry P, Lomenick B, Garbis SD, Moradian A, Hsieh-Wilson LC. bioRxiv. 2023 Jun 14:2023.06.13.541482. doi: 10.1101/2023.06.13.541482. Preprint. PubMed
Nodal flow transfers polycystin to determine mouse left-right asymmetry. Tanaka Y, Morozumi A, Hirokawa N. Dev Cell. 2023 Jun 27:S1534-5807(23)00275-7. doi: 10.1016/j.devcel.2023.06.002. PubMed
The autophagic protein FYCO1 controls TNFRSF10/TRAIL receptor induced apoptosis and is inactivated by CASP8 (caspase 8). Coppola V, Marino I, Warnken U, Falchi M, Pasquini L, Biffoni M, De Maria R, Haas TL. Autophagy. 2023 Jul 7:1-19. doi: 10.1080/15548627.2023.2229656. PubMed
Interventional hydrogel microsphere vaccine as an immune amplifier for activated antitumour immunity after ablation therapy. Liu X, Zhuang Y, Huang W, Wu Z, Chen Y, Shan Q, Zhang Y, Wu Z, Ding X, Qiu Z, Cui W, Wang Z. Nat Commun. 2023 Jul 11;14(1):4106. doi: 10.1038/s41467-023-39759-w. PubMed
Involvement of langerin in the protective function of a keratan sulfate-based disaccharide in an emphysema mouse model. Ohkawa Y, Kanto N, Nakano M, Fujinawa R, Kizuka Y, Johnson EL, Harada Y, Tamura JI, Taniguchi N. J Biol Chem. 2023 Jul 15;299(8):105052. doi: 10.1016/j.jbc.2023.105052. PubMed
OBOX regulates murine zygotic genome activation and early development. Ji S, Chen F, Stein P, Wang J, Zhou Z, Wang L, Zhao Q, Lin Z, Liu B, Xu K, Lai F, Xiong Z, Hu X, Kong T, Kong F, Huang B, Wang Q, Xu Q, Fan Q, Liu L, Williams CJ, Schultz RM, Xie W. Nature. 2023 Jul 17. doi: 10.1038/s41586-023-06428-3. PubMed
A functional sgRNA-CRISPR screening method for generating murine RET and NTRK1 rearranged oncogenes. Schubert L, Le AT, Hinz TK, Navarro AC, Nelson-Taylor SK, Nemenoff RA, Heasley LE, Doebele RC. Biol Open. 2023 Aug 15;12(8):bio059994. doi: 10.1242/bio.059994. Epub 2023 Aug 15. PubMed
PCDH12 loss results in premature neuronal differentiation and impeded migration in a cortical organoid model. Rakotomamonjy J, Rylaarsdam L, Fares-Taie L, McDermott S, Davies D, Yang G, Fagbemi F, Epstein M, Fairbanks-Santana M, Rozet JM, Guemez-Gamboa A. Cell Rep. 2023 Jul 20;42(8):112845. doi: 10.1016/j.celrep.2023.112845. PubMed
Rhox6 regulates the expression of distinct target genes to mediate mouse PGCLC formation and ESC self-renewal. Li X, Chen P, Ji J, Duan Q, Cao J, Huang R, Ye SD. Cell Biosci. 2023 Aug 8;13(1):145. doi: 10.1186/s13578-023-01096-2. PubMed
Optimized CRISPR/Cas9 system for gene knockout in chicken DF1 cells. Zou K, Wang F, Zhang Z, Zhou Y, Li P, Wang D, Zhu M, Jia C, Wei Z. Poult Sci. 2023 Oct;102(10):102970. doi: 10.1016/j.psj.2023.102970. Epub 2023 Jul 25. PubMed
Myddosome clustering in IL-1 receptor signaling regulates the formation of an NF-kB activating signalosome. Cao F, Deliz-Aguirre R, Gerpott FH, Ziska E, Taylor MJ. EMBO Rep. 2023 Oct 9;24(10):e57233. doi: 10.15252/embr.202357233. Epub 2023 Aug 21. PubMed
TRAIP resolves DNA replication-transcription conflicts during the S-phase of unperturbed cells. Scaramuzza S, Jones RM, Sadurni MM, Reynolds-Winczura A, Poovathumkadavil D, Farrell A, Natsume T, Rojas P, Cuesta CF, Kanemaki MT, Saponaro M, Gambus A. Nat Commun. 2023 Aug 21;14(1):5071. doi: 10.1038/s41467-023-40695-y. PubMed
The mTOR pathway genes mTOR, Rheb, Depdc5, Pten, and Tsc1 have convergent and divergent impacts on cortical neuron development and function. Nguyen LH, Xu Y, Nair M, Bordey A. bioRxiv [Preprint]. 2024 Jan 6:2023.08.11.553034. doi: 10.1101/2023.08.11.553034. PubMed
The non-redundant functions of PIWI family proteins in gametogenesis in golden hamsters. Lv X, Xiao W, Lai Y, Zhang Z, Zhang H, Qiu C, Hou L, Chen Q, Wang D, Gao Y, Song Y, Shui X, Chen Q, Qin R, Liang S, Zeng W, Shi A, Li J, Wu L. Nat Commun. 2023 Aug 29;14(1):5267. doi: 10.1038/s41467-023-40650-x. PubMed
An inducible germ cell ablation chicken model for high-grade germline chimeras. Chen YC, Saito D, Suzuki T, Takemoto T. Development. 2023 Sep 15;150(18):dev202079. doi: 10.1242/dev.202079. Epub 2023 Sep 25. PubMed
Pioneer activity distinguishes activating from non-activating SOX2 binding sites. Maresca M, van den Brand T, Li H, Teunissen H, Davies J, de Wit E. EMBO J. 2023 Oct 16;42(20):e113150. doi: 10.15252/embj.2022113150. Epub 2023 Sep 11. PubMed
Inducing multiple nicks promotes interhomolog homologous recombination to correct heterozygous mutations in somatic cells. Tomita A, Sasanuma H, Owa T, Nakazawa Y, Shimada M, Fukuoka T, Ogi T, Nakada S. Nat Commun. 2023 Sep 15;14(1):5607. doi: 10.1038/s41467-023-41048-5. PubMed
A cleavage rule for selection of increased-fidelity SpCas9 variants with high efficiency and no detectable off-targets. Kulcsar PI, Talas A, Ligeti Z, Toth E, Rakvacs Z, Bartos Z, Krausz SL, Welker A, Vegi VL, Huszar K, Welker E. Nat Commun. 2023 Sep 16;14(1):5746. doi: 10.1038/s41467-023-41393-5. PubMed Associated Plasmids HypaR-SpCas9 B-HypaR-SpCas9 B-Sniper SpCas9 B-HiFi SpCas9 B-evoSpCas9-V495M More ...
Development of a novel in vitro model to study the modulatory role of the respiratory complex I in macrophage effector functions. Serrano-Lorenzo P, Gobelli D, Garrido-Moraga R, Esteban-Amo MJ, Lopez-Lopez JR, Orduna A, de la Fuente MA, Martin MA, Simarro M. PLoS One. 2023 Sep 19;18(9):e0291442. doi: 10.1371/journal.pone.0291442. eCollection 2023. PubMed
Assembloid CRISPR screens reveal impact of disease genes in human neurodevelopment. Meng X, Yao D, Imaizumi K, Chen X, Kelley KW, Reis N, Thete MV, Arjun McKinney A, Kulkarni S, Panagiotakos G, Bassik MC, Pasca SP. Nature. 2023 Oct;622(7982):359-366. doi: 10.1038/s41586-023-06564-w. Epub 2023 Sep 27. PubMed
Kirigami electronics for long-term electrophysiological recording of human neural organoids and assembloids. Yang X, Forro C, Li TL, Miura Y, Zaluska TJ, Tsai CT, Kanton S, McQueen JP, Chen X, Mollo V, Santoro F, Pasca SP, Cui B. bioRxiv [Preprint]. 2023 Sep 22:2023.09.22.559050. doi: 10.1101/2023.09.22.559050. PubMed
Hepatitis C virus alters the morphology and function of peroxisomes. Martin de Fourchambault E, Callens N, Saliou JM, Fourcot M, Delos O, Barois N, Thorel Q, Ramirez S, Bukh J, Cocquerel L, Bertrand-Michel J, Marot G, Sebti Y, Dubuisson J, Rouille Y. Front Microbiol. 2023 Sep 21;14:1254728. doi: 10.3389/fmicb.2023.1254728. eCollection 2023. PubMed
The SMN complex drives structural changes in human snRNAs to enable snRNP assembly. Panek J, Roithova A, Radivojevic N, Sykora M, Prusty AB, Huston N, Wan H, Pyle AM, Fischer U, Stanek D. Nat Commun. 2023 Oct 18;14(1):6580. doi: 10.1038/s41467-023-42324-0. PubMed
The intrinsically disordered region of eIF5B stimulates IRES usage and nucleates biological granule formation. Harris MT, Marr MT 2nd. Cell Rep. 2023 Oct 31;42(10):113283. doi: 10.1016/j.celrep.2023.113283. Epub 2023 Oct 19. PubMed
Structural and functional basis of inositol hexaphosphate stimulation of NHEJ through stabilization of Ku-XLF interaction. Kefala Stavridi A, Gontier A, Morin V, Frit P, Ropars V, Barboule N, Racca C, Jonchhe S, Morten MJ, Andreani J, Rak A, Legrand P, Bourand-Plantefol A, Hardwick SW, Chirgadze DY, Davey P, De Oliveira TM, Rothenberg E, Britton S, Calsou P, Blundell TL, Varela PF, Chaplin AK, Charbonnier JB. Nucleic Acids Res. 2023 Nov 27;51(21):11732-11747. doi: 10.1093/nar/gkad863. PubMed
An integrative single-cell multi-omics profiling of human pancreatic islets identifies T1D associated genes and regulatory signals. Zhao Z, D'Oliveira Albanus R, Taylor H, Tang X, Han Y, Orchard P, Varshney A, Zhang T, Manickam N, Erdos M, Narisu N, Taylor L, Saavedra X, Zhong A, Li B, Zhou T, Naji A, Liu C, Collins F, Parker SC, Chen S. Res Sq [Preprint]. 2023 Oct 18:rs.3.rs-3343318. doi: 10.21203/rs.3.rs-3343318/v1. PubMed
The expression and function of HSV ICP47 and its promoter in mice. Velusamy T, Singh N, Croft S, Smith S, Tscharke DC. J Virol. 2023 Nov 30;97(11):e0110723. doi: 10.1128/jvi.01107-23. Epub 2023 Oct 30. PubMed
Optogenetic control of YAP reveals a dynamic communication code for stem cell fate and proliferation. Meyer K, Lammers NC, Bugaj LJ, Garcia HG, Weiner OD. Nat Commun. 2023 Oct 30;14(1):6929. doi: 10.1038/s41467-023-42643-2. PubMed
Mouse genome rewriting and tailoring of three important disease loci. Zhang W, Golynker I, Brosh R, Fajardo A, Zhu Y, Wudzinska AM, Ordonez R, Ribeiro-Dos-Santos AM, Carrau L, Damani-Yokota P, Yeung ST, Khairallah C, Vela Gartner A, Chalhoub N, Huang E, Ashe HJ, Khanna KM, Maurano MT, Kim SY, tenOever BR, Boeke JD. Nature. 2023 Nov 1. doi: 10.1038/s41586-023-06675-4. PubMed Associated Plasmids pWZ403 pWZ852 pWZ853 pWZ854 pWZ699
NR5A2 connects zygotic genome activation to the first lineage segregation in totipotent embryos. Lai F, Li L, Hu X, Liu B, Zhu Z, Liu L, Fan Q, Tian H, Xu K, Lu X, Li Q, Feng K, Wang L, Lin Z, Deng H, Li J, Xie W. Cell Res. 2023 Dec;33(12):952-966. doi: 10.1038/s41422-023-00887-z. Epub 2023 Nov 7. PubMed
StayGold variants for molecular fusion and membrane-targeting applications. Ando R, Shimozono S, Ago H, Takagi M, Sugiyama M, Kurokawa H, Hirano M, Niino Y, Ueno G, Ishidate F, Fujiwara T, Okada Y, Yamamoto M, Miyawaki A. Nat Methods. 2023 Nov 30. doi: 10.1038/s41592-023-02085-6. PubMed Associated Plasmids pRSETB/mStayGold pRSETB/mStayGold(c4) pcDNA3/F-tractin=mStayGold pcDNA3/mStayGold(c4)=UtrCH pcDNA3/td5StayGold(c4)=GianCreg
Altered Regulation of the Glucose Transporter GLUT3 in PRDX1 Null Cells Caused Hypersensitivity to Arsenite. Ali R, Alhaj Sulaiman A, Memon B, Pradhan S, Algethami M, Aouida M, McKay G, Madhusudan S, Abdelalim EM, Ramotar D. Cells. 2023 Nov 22;12(23):2682. doi: 10.3390/cells12232682. PubMed
Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Nakade K, Tsukamoto S, Nakashima K, An Y, Sato I, Li J, Shimoda Y, Hemmi Y, Miwa Y, Hayashi Y. Cell Rep Methods. 2023 Dec 18;3(12):100662. doi: 10.1016/j.crmeth.2023.100662. Epub 2023 Dec 11. PubMed
Pfs230 Domain 7 is targeted by a potent malaria transmission-blocking monoclonal antibody. Inklaar MR, de Jong RM, Bekkering ET, Nagaoka H, Fennemann FL, Teelen K, van de Vegte-Bolmer M, van Gemert GJ, Stoter R, King CR, Proellochs NI, Bousema T, Takashima E, Tsuboi T, Jore MM. NPJ Vaccines. 2023 Dec 12;8(1):186. doi: 10.1038/s41541-023-00784-x. PubMed
BCL-XL regulates the timing of mitotic apoptosis independently of BCL2 and MCL1 compensation. Yu CY, Yeung TK, Fu WK, Poon RYC. Cell Death Dis. 2024 Jan 3;15(1):2. doi: 10.1038/s41419-023-06404-9. PubMed
TREX2 deficiency suppresses spontaneous and genotoxin-associated mutagenesis. Marple T, Son MY, Cheng X, Ko JH, Sung P, Hasty P. Cell Rep. 2024 Jan 23;43(1):113637. doi: 10.1016/j.celrep.2023.113637. Epub 2024 Jan 3. PubMed
Somatic mouse models of gastric cancer reveal genotype-specific features of metastatic disease. Leibold J, Tsanov KM, Amor C, Ho YJ, Sanchez-Rivera FJ, Feucht J, Baslan T, Chen HA, Tian S, Simon J, Wuest A, Wilkinson JE, Lowe SW. Nat Cancer. 2024 Feb;5(2):315-329. doi: 10.1038/s43018-023-00686-w. Epub 2024 Jan 4. PubMed
Oligodendrocyte-derived LGI3 and its receptor ADAM23 organize juxtaparanodal Kv1 channel clustering for short-term synaptic plasticity. Miyazaki Y, Otsuka T, Yamagata Y, Endo T, Sanbo M, Sano H, Kobayashi K, Inahashi H, Kornau HC, Schmitz D, Pruss H, Meijer D, Hirabayashi M, Fukata Y, Fukata M. Cell Rep. 2024 Jan 23;43(1):113634. doi: 10.1016/j.celrep.2023.113634. Epub 2024 Jan 8. PubMed
The heterogeneity of signaling pathways and drug responses in intrahepatic cholangiocarcinoma with distinct genetic mutations. Feng Y, Zhao M, Wang L, Li L, Lei JH, Zhou J, Chen J, Wu Y, Miao K, Deng CX. Cell Death Dis. 2024 Jan 11;15(1):34. doi: 10.1038/s41419-023-06406-7. PubMed
An ovalbumin fusion strategy to increase recombinant protein secretion in chicken eggs. Xie L, Huang Z, Lan M, Cao Y, Sun L, Zhang L, Zuo E, Lu Y. J Biol Eng. 2024 Jan 11;18(1):5. doi: 10.1186/s13036-023-00390-4. PubMed
Generation of a patient-specific iPSC cell line with cardiac arrhythmias and dilated cardiomyopathy (CBRCULi016-A), an isogenic control (CBRCULi016-A-1), and a paternal control (CBRCULi017-A). Djemai M, Jauvin D, Poulin H, Chapotte-Baldacci CA, Chahine M. Stem Cell Res. 2024 Mar;75:103308. doi: 10.1016/j.scr.2024.103308. Epub 2024 Jan 9. PubMed
Methodology to Create Auxin-Inducible Degron Tagging System to Control Expression of a Target Protein in Mammalian Cell Lines. Rahi A, Sodhi DK, Magdongon CB, Shakya R, Varma D. Bio Protoc. 2024 Jan 20;14(2):e4923. doi: 10.21769/BioProtoc.4923. eCollection 2024 Jan 20. PubMed
CAG repeat expansions create splicing acceptor sites and produce aberrant repeat-containing RNAs. Anderson R, Das MR, Chang Y, Farenhem K, Schmitz CO, Jain A. Mol Cell. 2024 Feb 15;84(4):702-714.e10. doi: 10.1016/j.molcel.2024.01.006. Epub 2024 Jan 30. PubMed
Inhibition of protein kinase C increases Prdm14 level to promote self-renewal of embryonic stem cells through reducing Suv39h-induced H3K9 methylation. Ji J, Cao J, Chen P, Huang R, Ye SD. J Biol Chem. 2024 Mar;300(3):105714. doi: 10.1016/j.jbc.2024.105714. Epub 2024 Feb 2. PubMed
Coregulation of NDC80 Complex Subunits Determines the Fidelity of the Spindle-Assembly Checkpoint and Mitosis. Kim S, Lau TTY, Liao MK, Ma HT, Poon RYC. Mol Cancer Res. 2024 May 2;22(5):423-439. doi: 10.1158/1541-7786.MCR-23-0828. PubMed
RNA m(6)A modification regulates L1 retrotransposons in human spermatogonial stem cell differentiation in vitro and in vivo. Li Z, Fang F, Zafar MI, Wu X, Liu X, Tan X, Luo J, Ye Z, Xiong C, Li H. Cell Mol Life Sci. 2024 Feb 16;81(1):92. doi: 10.1007/s00018-024-05119-0. PubMed
Pro-ferroptotic signaling promotes arterial aging via vascular smooth muscle cell senescence. Sun DY, Wu WB, Wu JJ, Shi Y, Xu JJ, Ouyang SX, Chi C, Shi Y, Ji QX, Miao JH, Fu JT, Tong J, Zhang PP, Zhang JB, Li ZY, Qu LF, Shen FM, Li DJ, Wang P. Nat Commun. 2024 Feb 16;15(1):1429. doi: 10.1038/s41467-024-45823-w. PubMed
Sparse CBX2 nucleates many Polycomb proteins to promote facultative heterochromatinization of Polycomb target genes. Ingersoll S, Trouth A, Luo X, Espinoza A, Wen J, Tucker J, Astatike K, Phiel CJ, Kutateladze TG, Wu TP, Ramachandran S, Ren X. bioRxiv [Preprint]. 2024 Feb 5:2024.02.05.578969. doi: 10.1101/2024.02.05.578969. PubMed
DOT1L stimulates MYC/Mondo transcription factor activity by promoting its degradation cycle on chromatin. Sepulveda GP, Gushchanskaia ES, Mora-Martin A, Esse R, Nikorich I, Ceballos A, Kwan J, Blum BC, Dholiya P, Emili A, Perissi V, Cardamone MD, Grishok A. bioRxiv [Preprint]. 2024 Feb 7:2024.02.06.579191. doi: 10.1101/2024.02.06.579191. PubMed
Tunable DNMT1 degradation reveals DNMT1/DNMT3B synergy in DNA methylation and genome organization. Scelfo A, Barra V, Abdennur N, Spracklin G, Busato F, Salinas-Luypaert C, Bonaiti E, Velasco G, Bonhomme F, Chipont A, Tijhuis AE, Spierings DCJ, Guerin C, Arimondo P, Francastel C, Foijer F, Tost J, Mirny L, Fachinetti D. J Cell Biol. 2024 Apr 1;223(4):e202307026. doi: 10.1083/jcb.202307026. Epub 2024 Feb 20. PubMed
Undetectable off-target effects induced by FokI catalytic domain in mouse embryos. Xie L, Feng H, Li Z, Li D, Yang X, Yuan T, Yan N, He C, Zheng J, Zuo Z, Zheng Y, Cao Y, Lu Y, Xiong XY, Zuo E. Genome Biol. 2024 Feb 20;25(1):51. doi: 10.1186/s13059-024-03188-9. PubMed
Cullin-RING ligases employ geometrically optimized catalytic partners for substrate targeting. Li J, Purser N, Liwocha J, Scott DC, Byers HA, Steigenberger B, Hill S, Tripathi-Giesgen I, Hinkle T, Hansen FM, Prabu JR, Radhakrishnan SK, Kirkpatrick DS, Reichermeier KM, Schulman BA, Kleiger G. Mol Cell. 2024 Apr 4;84(7):1304-1320.e16. doi: 10.1016/j.molcel.2024.01.022. Epub 2024 Feb 20. PubMed
Mode of SUV420H2 heterochromatin localization through multiple HP1 binding motifs in the heterochromatic targeting module. Nakao M, Sato Y, Aizawa A, Kimura H. Genes Cells. 2024 May;29(5):361-379. doi: 10.1111/gtc.13109. Epub 2024 Feb 25. PubMed
Mouse SAS-6 is required for centriole formation in embryos and integrity in embryonic stem cells. Grzonka M, Bazzi H. Elife. 2024 Feb 26;13:e94694. doi: 10.7554/eLife.94694. PubMed
The mTOR pathway genes MTOR, Rheb, Depdc5, Pten, and Tsc1 have convergent and divergent impacts on cortical neuron development and function. Nguyen LH, Xu Y, Nair M, Bordey A. Elife. 2024 Feb 27;12:RP91010. doi: 10.7554/eLife.91010. PubMed
The transcription factor ChREBP Orchestrates liver carcinogenesis by coordinating the PI3K/AKT signaling and cancer metabolism. Benichou E, Seffou B, Topcu S, Renoult O, Lenoir V, Planchais J, Bonner C, Postic C, Prip-Buus C, Pecqueur C, Guilmeau S, Alves-Guerra MC, Dentin R. Nat Commun. 2024 Feb 29;15(1):1879. doi: 10.1038/s41467-024-45548-w. PubMed
Generation of Fel d 1 chain 2 genome-edited cats by CRISPR-Cas9 system. Lee SR, Lee KL, Song SH, Joo MD, Lee SH, Kang JS, Kang SM, Idrees M, Kim JW, Kong IK. Sci Rep. 2024 Feb 29;14(1):4987. doi: 10.1038/s41598-024-55464-0. PubMed
PolyGR and polyPR knock-in mice reveal a conserved neuroprotective extracellular matrix signature in C9orf72 ALS/FTD neurons. Milioto C, Carcole M, Giblin A, Coneys R, Attrebi O, Ahmed M, Harris SS, Lee BI, Yang M, Ellingford RA, Nirujogi RS, Biggs D, Salomonsson S, Zanovello M, de Oliveira P, Katona E, Glaria I, Mikheenko A, Geary B, Udine E, Vaizoglu D, Anoar S, Jotangiya K, Crowley G, Smeeth DM, Adams ML, Niccoli T, Rademakers R, van Blitterswijk M, Devoy A, Hong S, Partridge L, Coyne AN, Fratta P, Alessi DR, Davies B, Busche MA, Greensmith L, Fisher EMC, Isaacs AM. Nat Neurosci. 2024 Apr;27(4):643-655. doi: 10.1038/s41593-024-01589-4. Epub 2024 Feb 29. PubMed
Fatty acid-binding proteins 3, 7, and 8 bind cholesterol and facilitate its egress from lysosomes. Fang XX, Wei P, Zhao K, Sheng ZC, Song BL, Yin L, Luo J. J Cell Biol. 2024 Apr 1;223(4):e202211062. doi: 10.1083/jcb.202211062. Epub 2024 Mar 1. PubMed
RTF2 controls replication repriming and ribonucleotide excision at the replisome. Conti BA, Ruiz PD, Broton C, Blobel NJ, Kottemann MC, Sridhar S, Lach FP, Wiley TF, Sasi NK, Carroll T, Smogorzewska A. Nat Commun. 2024 Mar 2;15(1):1943. doi: 10.1038/s41467-024-45947-z. PubMed
LncRNA Litchi is a regulator for harmonizing maturity and resilient functionality in spinal motor neurons. Hsu HC, Hsu SP, Hsu FY, Chang M, Chen JA. iScience. 2024 Feb 10;27(3):109207. doi: 10.1016/j.isci.2024.109207. eCollection 2024 Mar 15. PubMed
A humanized mouse model for adeno-associated viral gene therapy. Barzi M, Chen T, Gonzalez TJ, Pankowicz FP, Oh SH, Streff HL, Rosales A, Ma Y, Collias S, Woodfield SE, Diehl AM, Vasudevan SA, Galvan TN, Goss J, Gersbach CA, Bissig-Choisat B, Asokan A, Bissig KD. Nat Commun. 2024 Mar 4;15(1):1955. doi: 10.1038/s41467-024-46017-0. PubMed
DJ-1 protects proteins from acylation by catalyzing the hydrolysis of highly reactive cyclic 3-phosphoglyceric anhydride. Akhmadi A, Yeskendir A, Dey N, Mussakhmetov A, Shatkenova Z, Kulyyassov A, Andreeva A, Utepbergenov D. Nat Commun. 2024 Mar 5;15(1):2004. doi: 10.1038/s41467-024-46391-9. PubMed
Structure and tethering mechanism of dynein-2 intermediate chains in intraflagellar transport. Mukhopadhyay AG, Toropova K, Daly L, Wells JN, Vuolo L, Mladenov M, Seda M, Jenkins D, Stephens DJ, Roberts AJ. EMBO J. 2024 Apr;43(7):1257-1272. doi: 10.1038/s44318-024-00060-1. Epub 2024 Mar 7. PubMed
SMARCA4 is a haploinsufficient B cell lymphoma tumor suppressor that fine-tunes centrocyte cell fate decisions. Deng Q, Lakra P, Gou P, Yang H, Meydan C, Teater M, Chin C, Zhang W, Dinh T, Hussein U, Li X, Rojas E, Liu W, Reville PK, Kizhakeyil A, Barisic D, Parsons S, Wilson A, Henderson J, Scull B, Gurumurthy C, Vega F, Chadburn A, Cuglievan B, El-Mallawany NK, Allen C, Mason C, Melnick A, Green MR. Cancer Cell. 2024 Apr 8;42(4):605-622.e11. doi: 10.1016/j.ccell.2024.02.011. Epub 2024 Mar 7. PubMed
IntAct: A nondisruptive internal tagging strategy to study the organization and function of actin isoforms. van Zwam MC, Dhar A, Bosman W, van Straaten W, Weijers S, Seta E, Joosten B, van Haren J, Palani S, van den Dries K. PLoS Biol. 2024 Mar 11;22(3):e3002551. doi: 10.1371/journal.pbio.3002551. eCollection 2024 Mar. PubMed
Inhibition of 7-dehydrocholesterol reductase prevents hepatic ferroptosis under an active state of sterol synthesis. Yamada N, Karasawa T, Ito J, Yamamuro D, Morimoto K, Nakamura T, Komada T, Baatarjav C, Saimoto Y, Jinnouchi Y, Watanabe K, Miura K, Yahagi N, Nakagawa K, Matsumura T, Yamada KI, Ishibashi S, Sata N, Conrad M, Takahashi M. Nat Commun. 2024 Mar 12;15(1):2195. doi: 10.1038/s41467-024-46386-6. PubMed
Prediction and validation of circulating G-quadruplexes as a novel biomarker in colorectal cancer. Zhang H, Zhou J, Ye Y. J Gastrointest Oncol. 2024 Feb 29;15(1):286-298. doi: 10.21037/jgo-24-26. Epub 2024 Feb 28. PubMed
Endogenous BAX and BAK form mosaic rings of variable size and composition on apoptotic mitochondria. Schweighofer SV, Jans DC, Keller-Findeisen J, Folmeg A, Ilgen P, Bates M, Jakobs S. Cell Death Differ. 2024 Apr;31(4):469-478. doi: 10.1038/s41418-024-01273-x. Epub 2024 Mar 19. PubMed
Engineered FSHD mutations results in D4Z4 heterochromatin disruption and feedforward DUX4 network activation. Kong X, Nguyen NV, Li Y, Sakr JS, Williams K, Sharifi S, Chau J, Bayrakci A, Mizuno S, Takahashi S, Kiyono T, Tawil R, Mortazavi A, Yokomori K. iScience. 2024 Feb 29;27(4):109357. doi: 10.1016/j.isci.2024.109357. eCollection 2024 Apr 19. PubMed
Oligomerization mediated by the D2 domain of DTX3L is critical for DTX3L-PARP9 reading function of mono-ADP-ribosylated androgen receptor. Vela-Rodriguez C, Yang C, Alanen HI, Eki R, Abbas TA, Maksimainen MM, Glumoff T, Duman R, Wagner A, Paschal BM, Lehtio L. Protein Sci. 2024 Apr;33(4):e4945. doi: 10.1002/pro.4945. PubMed
Antigen-independent, autonomous B cell receptor signaling drives activated B cell DLBCL. Eken JA, Koning MT, Kupcova K, Sepulveda Yanez JH, de Groen RAL, Quinten E, Janssen J, van Bergen CAM, Vermaat JSP, Cleven A, Navarrete MA, Ylstra B, de Jong D, Havranek O, Jumaa H, Veelken H. J Exp Med. 2024 May 6;221(5):e20230941. doi: 10.1084/jem.20230941. Epub 2024 Mar 21. PubMed
Molecular basis promoting centriole triplet microtubule assembly. Takeda Y, Chinen T, Honda S, Takatori S, Okuda S, Yamamoto S, Fukuyama M, Takeuchi K, Tomita T, Hata S, Kitagawa D. Nat Commun. 2024 Mar 22;15(1):2216. doi: 10.1038/s41467-024-46454-x. PubMed
Cancer cell genetics shaping of the tumor microenvironment reveals myeloid cell-centric exploitable vulnerabilities in hepatocellular carcinoma. Ramirez CFA, Taranto D, Ando-Kuri M, de Groot MHP, Tsouri E, Huang Z, de Groot D, Kluin RJC, Kloosterman DJ, Verheij J, Xu J, Vegna S, Akkari L. Nat Commun. 2024 Mar 22;15(1):2581. doi: 10.1038/s41467-024-46835-2. PubMed
Mitophagy mediated by BNIP3 and NIX protects against ferroptosis by downregulating mitochondrial reactive oxygen species. Yamashita SI, Sugiura Y, Matsuoka Y, Maeda R, Inoue K, Furukawa K, Fukuda T, Chan DC, Kanki T. Cell Death Differ. 2024 May;31(5):651-661. doi: 10.1038/s41418-024-01280-y. Epub 2024 Mar 22. PubMed
Depletion of lamins B1 and B2 promotes chromatin mobility and induces differential gene expression by a mesoscale-motion-dependent mechanism. Pujadas Liwag EM, Wei X, Acosta N, Carter LM, Yang J, Almassalha LM, Jain S, Daneshkhah A, Rao SSP, Seker-Polat F, MacQuarrie KL, Ibarra J, Agrawal V, Aiden EL, Kanemaki MT, Backman V, Adli M. Genome Biol. 2024 Mar 22;25(1):77. doi: 10.1186/s13059-024-03212-y. PubMed
An oocyte-specific Cas9-expressing mouse for germline CRISPR/Cas9-mediated genome editing. Lanza DG, Mao J, Lorenzo I, Liao L, Seavitt JR, Ljungberg MC, Simpson EM, DeMayo FJ, Heaney JD. Genesis. 2024 Apr;62(2):e23589. doi: 10.1002/dvg.23589. PubMed
The genetically modified human foreskin fibroblast cell line (YhFF#8) stably expressing Cas9 gene: A lab resource report. Soheilipour F, Boozarpour S, Aghaei S, Farashahi Yazd E. Int J Reprod Biomed. 2024 Feb 23;22(1):61-68. doi: 10.18502/ijrm.v22i1.15243. eCollection 2024 Jan. PubMed
Structure and function of Semaphorin-5A glycosaminoglycan interactions. Nagy GN, Zhao XF, Karlsson R, Wang K, Duman R, Harlos K, El Omari K, Wagner A, Clausen H, Miller RL, Giger RJ, Jones EY. Nat Commun. 2024 Mar 28;15(1):2723. doi: 10.1038/s41467-024-46725-7. PubMed
Mycobacterium tuberculosis inhibits METTL14-mediated m(6)A methylation of Nox2 mRNA and suppresses anti-TB immunity. Ma M, Duan Y, Peng C, Wu Y, Zhang X, Chang B, Wang F, Yang H, Zheng R, Cheng H, Cheng Y, He Y, Huang J, Lei J, Ma H, Li L, Wang J, Huang X, Tang F, Liu J, Li J, Ying R, Wang P, Sha W, Gao Y, Wang L, Ge B. Cell Discov. 2024 Mar 29;10(1):36. doi: 10.1038/s41421-024-00653-4. PubMed
A Trisomy 21-linked Hematopoietic Gene Variant in Microglia Confers Resilience in Human iPSC Models of Alzheimer's Disease. Jin M, Ma Z, Dang R, Zhang H, Kim R, Xue H, Pascual J, Finkbeiner S, Head E, Liu Y, Jiang P. bioRxiv [Preprint]. 2024 Mar 14:2024.03.12.584646. doi: 10.1101/2024.03.12.584646. PubMed
ER-export and ARFRP1/AP-1-dependent delivery of SARS-CoV-2 Envelope to lysosomes controls late stages of viral replication. Pearson GJ, Mears HV, Broncel M, Snijders AP, Bauer DLV, Carlton JG. Sci Adv. 2024 Apr 5;10(14):eadl5012. doi: 10.1126/sciadv.adl5012. Epub 2024 Apr 3. PubMed
RAD51 separation of function mutation disables replication fork maintenance but preserves DSB repair. Son MY, Belan O, Spirek M, Cibulka J, Nikulenkov F, Kim YY, Hwang S, Myung K, Montagna C, Kim TM, Krejci L, Hasty P. iScience. 2024 Mar 16;27(4):109524. doi: 10.1016/j.isci.2024.109524. eCollection 2024 Apr 19. PubMed
Non-canonical functions of UHRF1 maintain DNA methylation homeostasis in cancer cells. Yamaguchi K, Chen X, Rodgers B, Miura F, Bashtrykov P, Bonhomme F, Salinas-Luypaert C, Haxholli D, Gutekunst N, Aygenli BO, Ferry L, Kirsh O, Laisne M, Scelfo A, Ugur E, Arimondo PB, Leonhardt H, Kanemaki MT, Bartke T, Fachinetti D, Jeltsch A, Ito T, Defossez PA. Nat Commun. 2024 Apr 5;15(1):2960. doi: 10.1038/s41467-024-47314-4. PubMed
Generation of human cerebral organoids with a structured outer subventricular zone. Walsh RM, Luongo R, Giacomelli E, Ciceri G, Rittenhouse C, Verrillo A, Galimberti M, Bocchi VD, Wu Y, Xu N, Mosole S, Muller J, Vezzoli E, Jungverdorben J, Zhou T, Barker RA, Cattaneo E, Studer L, Baggiolini A. Cell Rep. 2024 Apr 23;43(4):114031. doi: 10.1016/j.celrep.2024.114031. Epub 2024 Apr 6. PubMed
The impact of canonical Wnt transcriptional repressors TLE3 and TLE4 on postsynaptic transcription at the neuromuscular junction. Gessler L, Huraskin D, Eiber N, Hashemolhosseini S. Front Mol Neurosci. 2024 Mar 27;17:1360368. doi: 10.3389/fnmol.2024.1360368. eCollection 2024. PubMed
Luciferase- and HaloTag-based reporter assays to measure small-molecule-induced degradation pathway in living cells. Schwalm MP, Saxena K, Muller S, Knapp S. Nat Protoc. 2024 Apr 18. doi: 10.1038/s41596-024-00979-z. PubMed
Transcriptomic profiling across human serotonin neuron differentiation via the FEV reporter system. Li Y, Duan J, Li Y, Zhang M, Wu J, Wang G, Li S, Hu Z, Qu Y, Li Y, Hu X, Guo F, Cao L, Lu J. Stem Cell Res Ther. 2024 Apr 19;15(1):107. doi: 10.1186/s13287-024-03728-x. PubMed
Ki-67 is necessary during DNA replication for fork protection and genome stability. Stamatiou K, Huguet F, Serapinas LV, Spanos C, Rappsilber J, Vagnarelli P. Genome Biol. 2024 Apr 22;25(1):105. doi: 10.1186/s13059-024-03243-5. PubMed
Linear interaction between replication and transcription shapes DNA break dynamics at recurrent DNA break Clusters. Corazzi L, Ionasz VS, Andrejev S, Wang LC, Vouzas A, Giaisi M, Di Muzio G, Ding B, Marx AJM, Henkenjohann J, Allers MM, Gilbert DM, Wei PC. Nat Commun. 2024 Apr 27;15(1):3594. doi: 10.1038/s41467-024-47934-w. PubMed
WNT5B drives osteosarcoma stemness, chemoresistance and metastasis. Perkins RS, Murray G, Suthon S, Davis L, Perkins NB 3rd, Fletcher L, Bozzi A, Schreiber SL, Lin J, Laxton S, Pillai RR, Wright AJ, Miranda-Carboni GA, Krum SA. Clin Transl Med. 2024 May;14(5):e1670. doi: 10.1002/ctm2.1670. PubMed
The factor inhibiting HIF regulates T cell differentiation and anti-tumour efficacy. Bargiela D, Cunha PP, Velica P, Krause LCM, Brice M, Barbieri L, Gojkovic M, Foskolou IP, Rundqvist H, Johnson RS. Front Immunol. 2024 Apr 16;15:1293723. doi: 10.3389/fimmu.2024.1293723. eCollection 2024. PubMed
CD74 supports accumulation and function of regulatory T cells in tumors. Bonnin E, Rodrigo Riestra M, Marziali F, Mena Osuna R, Denizeau J, Maurin M, Saez JJ, Jouve M, Bonte PE, Richer W, Nevo F, Lemoine S, Girard N, Lefevre M, Borcoman E, Vincent-Salomon A, Baulande S, Moreau HD, Sedlik C, Hivroz C, Lennon-Dumenil AM, Tosello Boari J, Piaggio E. Nat Commun. 2024 May 3;15(1):3749. doi: 10.1038/s41467-024-47981-3. PubMed
HIF-1alpha participates in the regulation of S100A16-HRD1-GSK3beta/CK1alpha pathway in renal hypoxia injury. Han S, Jin R, Huo L, Teng Y, Zhao L, Zhang K, Li R, Su D, Liang X. Cell Death Dis. 2024 May 6;15(5):316. doi: 10.1038/s41419-024-06696-5. PubMed
Blockage of BCL-XL overcomes venetoclax resistance across BCL2+ lymphoid malignancies irrespective of BIM status. Dolnikova A, Kazantsev D, Klanova M, Pokorna E, Sovilj D, Kelemen CD, Tuskova L, Hoferkova E, Mraz M, Helman K, Curik N, Machova Polakova K, Andera L, Trneny M, Klener P. Blood Adv. 2024 Jul 9;8(13):3532-3543. doi: 10.1182/bloodadvances.2024012906. PubMed
A temperature-sensitive and interferon-silent Sendai virus vector for CRISPR-Cas9 delivery and gene editing in primary human cells. Stevens CS, Carmichael J, Watkinson R, Kowdle S, Reis RA, Hamane K, Jang J, Park A, Pernet O, Khamaikawin W, Hong P, Thibault P, Gowlikar A, An DS, Lee B. bioRxiv [Preprint]. 2024 May 5:2024.05.03.592383. doi: 10.1101/2024.05.03.592383. PubMed
Bag1 protein loss sensitizes mouse embryonic fibroblasts to glutathione depletion. Inose-Maruyama A, Irokawa H, Takeda K, Taguchi K, Morita M, Yamamoto M, Sasaki M, Kuge S. Cell Stress Chaperones. 2024 Jun;29(3):497-509. doi: 10.1016/j.cstres.2024.05.003. Epub 2024 May 17. PubMed
Mammalian RNAi represses pericentromeric lncRNAs to maintain genome stability. Sandoval R, Dilsavor CN, Grishanina NR, Patel V, Zamudio JR. bioRxiv [Preprint]. 2024 May 24:2024.05.09.593425. doi: 10.1101/2024.05.09.593425. PubMed
An antifouling membrane-fusogenic liposome for effective intracellular delivery in vivo. Kong H, Zheng C, Yi K, Mintz RL, Lao YH, Tao Y, Li M. Nat Commun. 2024 May 20;15(1):4267. doi: 10.1038/s41467-024-46533-z. PubMed
Pseudopregnant mice generated from Piwil1 deficiency sterile mice. Xie S, Qin R, Zeng W, Li J, Lai Y. PLoS One. 2024 May 21;19(5):e0296414. doi: 10.1371/journal.pone.0296414. eCollection 2024. PubMed
Ciliary tip actin dynamics regulate photoreceptor outer segment integrity. Megaw R, Moye A, Zhang Z, Newton F, McPhie F, Murphy LC, McKie L, He F, Jungnickel MK, von Kriegsheim A, Tennant PA, Brotherton C, Gurniak C, Gross AK, Machesky LM, Wensel TG, Mill P. Nat Commun. 2024 May 21;15(1):4316. doi: 10.1038/s41467-024-48639-w. PubMed
A global gene regulatory program and its region-specific regulator partition neurons into commissural and ipsilateral projection types. Masuda A, Nishida K, Ajima R, Saga Y, Bakhtan M, Klar A, Hirata T, Zhu Y. Sci Adv. 2024 May 24;10(21):eadk2149. doi: 10.1126/sciadv.adk2149. Epub 2024 May 23. PubMed
FokI-RYdCas9 Mediates Nearly PAM-Less and High-Precise Gene Editing in Human Cells. Li D, Cao Y, Xie L, He C, Jiao D, Ma M, Zuo Z, Zuo E, Yang X. Curr Issues Mol Biol. 2024 Apr 27;46(5):4021-4034. doi: 10.3390/cimb46050248. PubMed
H2AX promotes replication fork degradation and chemosensitivity in BRCA-deficient tumours. Dibitetto D, Liptay M, Vivalda F, Dogan H, Gogola E, Gonzalez Fernandez M, Duarte A, Schmid JA, Decollogny M, Francica P, Przetocka S, Durant ST, Forment JV, Klebic I, Siffert M, de Bruijn R, Kousholt AN, Marti NA, Dettwiler M, Sorensen CS, Tille JC, Undurraga M, Labidi-Galy I, Lopes M, Sartori AA, Jonkers J, Rottenberg S. Nat Commun. 2024 May 24;15(1):4430. doi: 10.1038/s41467-024-48715-1. PubMed
Endogenous oncogenic KRAS expression increases cell proliferation and motility in near-diploid hTERT RPE-1 cells. Hindul NL, Abbott LR, Adan SMD, Straatman KR, Fry AM, Hirota K, Tanaka K. J Biol Chem. 2024 Jun;300(6):107409. doi: 10.1016/j.jbc.2024.107409. Epub 2024 May 23. PubMed
Dynamic movement of the Golgi unit and its glycosylation enzyme zones. Harada A, Kunii M, Kurokawa K, Sumi T, Kanda S, Zhang Y, Nadanaka S, Hirosawa KM, Tokunaga K, Tojima T, Taniguchi M, Moriwaki K, Yoshimura SI, Yamamoto-Hino M, Goto S, Katagiri T, Kume S, Hayashi-Nishino M, Nakano M, Miyoshi E, Suzuki KGN, Kitagawa H, Nakano A. Nat Commun. 2024 May 27;15(1):4514. doi: 10.1038/s41467-024-48901-1. PubMed
Phosphorylation of DNA-binding domains of CLOCK-BMAL1 complex for PER-dependent inhibition in circadian clock of mammalian cells. Otobe Y, Jeong EM, Ito S, Shinohara Y, Kurabayashi N, Aiba A, Fukada Y, Kim JK, Yoshitane H. Proc Natl Acad Sci U S A. 2024 Jun 4;121(23):e2316858121. doi: 10.1073/pnas.2316858121. Epub 2024 May 28. PubMed
Single-molecule imaging reveals the kinetics of non-homologous end-joining in living cells. Mikhova M, Goff NJ, Janovic T, Heyza JR, Meek K, Schmidt JC. bioRxiv [Preprint]. 2024 May 25:2023.06.22.546088. doi: 10.1101/2023.06.22.546088. PubMed
Activation of NPY2R-expressing amygdala neurons inhibits itch behavior in mice without lateralization. Pavlenko D, Seven ZT, Sanders K, Markan A, Verpile R, Ishida H, Costco D, Akiyama T. Res Sq [Preprint]. 2024 May 24:rs.3.rs-4463812. doi: 10.21203/rs.3.rs-4463812/v1. PubMed
A suite of genome-engineered hepatic cells provides novel insights into the spatiotemporal metabolism of apolipoprotein B and apolipoprotein B-containing lipoprotein secretion. Meurs A, Ndoj K, van den Berg M, Marinkovic G, Tantucci M, Veenendaal T, Kuivenhoven JA, Klumperman J, Zelcer N. Cardiovasc Res. 2024 Sep 21;120(11):1253-1264. doi: 10.1093/cvr/cvae121. PubMed
Cancer-specific epigenome identifies oncogenic hijacking by nuclear factor I family proteins for medulloblastoma progression. Shiraishi R, Cancila G, Kumegawa K, Torrejon J, Basili I, Bernardi F, Silva PBGD, Wang W, Chapman O, Yang L, Jami M, Nishitani K, Arai Y, Xiao Z, Yu H, Lo Re V, Marsaud V, Talbot J, Lombard B, Loew D, Jingu M, Okonechnikov K, Sone M, Motohashi N, Aoki Y, Pfister SM, Chavez L, Hoshino M, Maruyama R, Ayrault O, Kawauchi D. Dev Cell. 2024 May 31:S1534-5807(24)00330-7. doi: 10.1016/j.devcel.2024.05.013. PubMed
A mechanistic study on the tolerance of PAM distal end mismatch by SpCas9. Dey D, Chakravarti R, Bhattacharjee O, Majumder S, Chaudhuri D, Ahmed KT, Roy D, Bhattacharya B, Arya M, Gautam A, Singh R, Gupta R, Ravichandiran V, Chattopadhyay D, Ghosh A, Giri K, Roy S, Ghosh D. J Biol Chem. 2024 Jul;300(7):107439. doi: 10.1016/j.jbc.2024.107439. Epub 2024 Jun 3. PubMed
Limited Immunogenicity of an HLA-A*03:01-restricted Epitope of Erv-k-env in Non-hiv-1 Settings: Implications for Adoptive Cell Therapy in Cancer. Grundy EE, Shaw LC, Wang L, Powell DJ, Ostrowski M, Jones RB, Cruz CRY, Gordish-Dressman H, Bollard CM, Chiappinelli KB. Res Sq [Preprint]. 2024 May 30:rs.3.rs-4432372. doi: 10.21203/rs.3.rs-4432372/v1. PubMed
Induction of Meiosis from Human Pluripotent Stem Cells. Pierson Smela M, Adams J, Ma C, Breimann L, Widocki U, Shioda T, Church GM. bioRxiv [Preprint]. 2024 May 31:2024.05.31.596483. doi: 10.1101/2024.05.31.596483. PubMed
K128 ubiquitination constrains RAS activity by expanding its binding interface with GAP proteins. Magits W, Steklov M, Jang H, Sewduth RN, Florentin A, Lechat B, Sheryazdanova A, Zhang M, Simicek M, Prag G, Nussinov R, Sablina A. EMBO J. 2024 Jul;43(14):2862-2877. doi: 10.1038/s44318-024-00146-w. Epub 2024 Jun 10. PubMed
Alternative splicing coupled to nonsense-mediated decay coordinates downregulation of non-neuronal genes in developing mouse neurons. Zhuravskaya A, Yap K, Hamid F, Makeyev EV. Genome Biol. 2024 Jun 20;25(1):162. doi: 10.1186/s13059-024-03305-8. PubMed
A suite of enhancer AAVs and transgenic mouse lines for genetic access to cortical cell types. Ben-Simon Y, Hooper M, Narayan S, Daigle TL, Dwivedi D, Way SW, Oster A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya X, Roth JR, Alexander JR, Allen S, Amster A, Arbuckle J, Ayala A, Baker PM, Bakken TE, Barcelli T, Barta S, Bendrick J, Bertagnolli D, Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, Casper T, Chakka AB, Chakrabarty R, Chance RK, Chavan S, Clark M, Colbert K, Collman F, Daniel S, Departee M, DiValentin P, Donadio N, Dotson N, Egdorf T, Fliss T, Gabitto M, Garcia J, Gary A, Gasperini M, Gloe J, Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, Halterman C, Haradon Z, Hastings SD, Helback O, Ho W, Hockemeyer D, Huang C, Huff S, Hunker A, Johansen N, Jones D, Juneau Z, Kalmbach B, Kannan M, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, Lee AY, Leibly M, Lenz GH, Li S, Liang E, Lusk N, Madigan Z, Malloy J, Malone J, McCue R, Melchor J, Mollenkopf T, Moosman S, Morin E, Newman D, Ng L, Ngo K, Omstead V, Otto S, Oyama A, Pena N, Pham T, Phillips E, Pom CA, Potekhina L, Ransford S, Ray PL, Rette D, Reynoldson C, Rimorin C, Rocha D, Ruiz A, Sanchez REA, Sawyer L, Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shepard N, Shulga L, Sigler AR, Siverts L, Soliman S, Somasundaram S, Staats B, Stewart K, Szelenyi E, Tieu M, Trader C, Tran A, van Velthoven CTJ, Walker M, Wang Y, Weed N, Wirthlin M, Wood T, Wynalda B, Yao Z, Zhou T, Ariza J, Dee N, Reding M, Ronellenfitch K, Mufti S, Sunkin SM, Smith KA, Esposito L, Waters J, Thyagarajan B, Yao S, Lein ES, Zeng H, Levi BP, Ngai J, Ting JT, Tasic B. Cell. 2025 May 16:S0092-8674(25)00513-6. doi: 10.1016/j.cell.2025.05.002. PubMed Associated Plasmids AiP11244 - pAAV-hDLXI56i-minBG-SYFP2-WPRE3-BGHpA (Alias: CN1244) AiP11402-pAAV-AiE0058h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN1402) AiP11457 - pAAV-eHGT_078h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN1457) AiP11253 - pAAV-eHGT_017h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN1253) AiP11621 - pAAV-hsA2-eHGT_128h-minRho-SYFP2-WPRE3-BGHpA (Alias: CN1621) More ...
RECON gene disruption enhances host resistance to enable genome-wide evaluation of intracellular pathogen fitness during infection. Stamm CE, McFarland AP, Locke MN, Tabakh H, Tang Q, Thomason MK, Woodward JJ. mBio. 2024 Aug 14;15(8):e0133224. doi: 10.1128/mbio.01332-24. Epub 2024 Jun 28. PubMed
MAST4 regulates stem cell maintenance with DLX3 for epithelial development and amelogenesis. Lee DJ, Kim P, Kim HY, Park J, Lee SJ, An H, Heo JS, Lee MJ, Ohshima H, Mizuno S, Takahashi S, Jung HS, Kim SJ. Exp Mol Med. 2024 Jul;56(7):1606-1619. doi: 10.1038/s12276-024-01264-5. Epub 2024 Jul 1. PubMed
Dual-specificity phosphatases 13 and 27 as key switches in muscle stem cell transition from proliferation to differentiation. Hayashi T, Sadaki S, Tsuji R, Okada R, Fuseya S, Kanai M, Nakamura A, Okamura Y, Muratani M, Wenchao G, Sugasawa T, Mizuno S, Warabi E, Kudo T, Takahashi S, Fujita R. Stem Cells. 2024 Sep 10;42(9):830-847. doi: 10.1093/stmcls/sxae045. PubMed
Human promoter directionality is determined by transcriptional initiation and the opposing activities of INTS11 and CDK9. Eaton JD, Board J, Davidson L, Estell C, West S. Elife. 2024 Jul 8;13:RP92764. doi: 10.7554/eLife.92764. PubMed
Electrophysiology of Human iPSC-derived Vascular Smooth Muscle Cells and Cell-autonomous Consequences of Cantu Syndrome Mutations. Hanson A, McClenaghan C, Weng KC, Colijn S, Stratman AN, Halabi CM, Grange DK, Silva JR, Nichols CG. Function (Oxf). 2024 Sep 10;5(5):zqae027. doi: 10.1093/function/zqae027. PubMed
FANCM promotes PARP inhibitor resistance by minimizing ssDNA gap formation and counteracting resection inhibition. Liu Z, Jiang H, Lee SY, Kong N, Chan YW. Cell Rep. 2024 Jul 23;43(7):114464. doi: 10.1016/j.celrep.2024.114464. Epub 2024 Jul 8. PubMed
CyCoNP lncRNA establishes cis and trans RNA-RNA interactions to supervise neuron physiology. Desideri F, Grazzi A, Lisi M, Setti A, Santini T, Colantoni A, Proietti G, Carvelli A, Tartaglia GG, Ballarino M, Bozzoni I. Nucleic Acids Res. 2024 Sep 9;52(16):9936-9952. doi: 10.1093/nar/gkae590. PubMed
Generation of MBP-tdTomato reporter human induced pluripotent stem cell line for live myelin visualization. Takagi D, Tsukamoto S, Nakade K, Shimizu T, Arai Y, Matsuo-Takasaki M, Noguchi M, Nakamura Y, Yumoto N, Kawada J, Hayata T, Hayashi Y. Stem Cell Res. 2024 Sep;79:103493. doi: 10.1016/j.scr.2024.103493. Epub 2024 Jul 11. PubMed
When less is more - a fast TurboID knock-in approach for high-sensitivity endogenous interactome mapping. Stockhammer A, Spalt C, Klemt A, Benz LS, Harel S, Natalia V, Wiench L, Freund C, Kuropka B, Bottanelli F. J Cell Sci. 2024 Aug 15;137(16):jcs261952. doi: 10.1242/jcs.261952. Epub 2024 Aug 28. PubMed Associated Plasmids AP1muA-TurboID-polyA-G418 HR AP1muA single guide RNA
Molecular Basis for the Calcium-Dependent Activation of the Ribonuclease EndoU. Malard F, Dias K, Baudy M, Thore S, Vialet B, Barthelemy P, Fribourg S, Karginov FV, Campagne S. Res Sq [Preprint]. 2024 Jul 15:rs.3.rs-4654759. doi: 10.21203/rs.3.rs-4654759/v1. PubMed
A novel chemical genetic approach reveals paralog-specific role of ERK1/2 in mouse embryonic stem cell fate control. Hu L, Xiao X, Huang W, Zhou T, Chen W, Zhang C, Ying QL. Front Cell Dev Biol. 2024 Jul 12;12:1415621. doi: 10.3389/fcell.2024.1415621. eCollection 2024. PubMed
SNX32 Regulates Sorting and Trafficking of Activated EGFR to the Lysosomal Degradation Pathway. Wang D, Zhao X, Wang P, Liu JJ. Traffic. 2024 Jul;25(7):e12952. doi: 10.1111/tra.12952. PubMed
Kinesins regulate the heterogeneity in centrosome clustering after whole-genome duplication. Lau TT, Ma HT, Poon RY. Life Sci Alliance. 2024 Jul 29;7(10):e202402670. doi: 10.26508/lsa.202402670. Print 2024 Oct. PubMed
Golgi pH homeostasis stabilizes the lysosomal membrane through N-glycosylation of membrane proteins. Sou YS, Yamaguchi J, Masuda K, Uchiyama Y, Maeda Y, Koike M. Life Sci Alliance. 2024 Jul 30;7(10):e202402677. doi: 10.26508/lsa.202402677. Print 2024 Oct. PubMed
MYC and p53 alterations cooperate through VEGF signaling to repress cytotoxic T cell and immunotherapy responses in prostate cancer. Murphy KC, DeMarco KD, Zhou L, Lopez-Diaz Y, Ho YJ, Li J, Bai S, Simin K, Zhu LJ, Mercurio AM, Ruscetti M. bioRxiv [Preprint]. 2024 Jul 24:2024.07.24.604943. doi: 10.1101/2024.07.24.604943. PubMed
Axo-axonic synaptic input drives homeostatic plasticity by tuning the axon initial segment structurally and functionally. Zhao R, Ren B, Xiao Y, Tian J, Zou Y, Wei J, Qi Y, Hu A, Xie X, Huang ZJ, Shu Y, He M, Lu J, Tai Y. Sci Adv. 2024 Aug 2;10(31):eadk4331. doi: 10.1126/sciadv.adk4331. Epub 2024 Aug 2. PubMed
Senataxin RNA/DNA helicase promotes replication restart at co-transcriptional R-loops to prevent MUS81-dependent fork degradation. Rao S, Andrs M, Shukla K, Isik E, Konig C, Schneider S, Bauer M, Rosano V, Prokes J, Muller A, Janscak P. Nucleic Acids Res. 2024 Sep 23;52(17):10355-10369. doi: 10.1093/nar/gkae673. PubMed
Primate-specific BTN3A2 protects against SARS-CoV-2 infection by interacting with and reducing ACE2. Xu L, Yu D, Xu M, Liu Y, Yang LX, Zou QC, Feng XL, Li MH, Sheng N, Yao YG. EBioMedicine. 2024 Sep;107:105281. doi: 10.1016/j.ebiom.2024.105281. Epub 2024 Aug 13. PubMed
Functions of PMS2 and MLH1 important for regulation of divergent repeat-mediated deletions. Trost H, Lopezcolorado FW, Merkell A, Stark JM. bioRxiv [Preprint]. 2024 Aug 6:2024.08.05.606388. doi: 10.1101/2024.08.05.606388. PubMed
Rapid phagosome isolation enables unbiased multiomic analysis of human microglial phagosomes. Wogram E, Sumpelmann F, Dong W, Rawat E, Fernandez Maestre I, Fu D, Braswell B, Khalil A, Buescher JM, Mittler G, Borner GHH, Vlachos A, Tholen S, Schilling O, Bell GW, Rambold AS, Akhtar A, Schnell O, Beck J, Abu-Remaileh M, Prinz M, Jaenisch R. Immunity. 2024 Sep 10;57(9):2216-2231.e11. doi: 10.1016/j.immuni.2024.07.019. Epub 2024 Aug 15. PubMed
BEAM: A combinatorial recombinase toolbox for binary gene expression and mosaic genetic analysis. Greig LC, Woodworth MB, Poulopoulos A, Lim S, Macklis JD. Cell Rep. 2024 Aug 27;43(8):114650. doi: 10.1016/j.celrep.2024.114650. Epub 2024 Aug 17. PubMed
Single-nucleosome imaging unveils that condensins and nucleosome-nucleosome interactions differentially constrain chromatin to organize mitotic chromosomes. Hibino K, Sakai Y, Tamura S, Takagi M, Minami K, Natsume T, Shimazoe MA, Kanemaki MT, Imamoto N, Maeshima K. Nat Commun. 2024 Aug 21;15(1):7152. doi: 10.1038/s41467-024-51454-y. PubMed
Tuberculosis in otherwise healthy adults with inherited TNF deficiency. Arias AA, Neehus AL, Ogishi M, Meynier V, Krebs A, Lazarov T, Lee AM, Arango-Franco CA, Yang R, Orrego J, Corcini Berndt M, Rojas J, Li H, Rinchai D, Erazo-Borras L, Han JE, Pillay B, Ponsin K, Chaldebas M, Philippot Q, Bohlen J, Rosain J, Le Voyer T, Janotte T, Amarajeeva K, Soudee C, Brollo M, Wiegmann K, Marquant Q, Seeleuthner Y, Lee D, Laine C, Kloos D, Bailey R, Bastard P, Keating N, Rapaport F, Khan T, Moncada-Velez M, Carmona MC, Obando C, Alvarez J, Catano JC, Martinez-Rosado LL, Sanchez JP, Tejada-Giraldo M, L'Honneur AS, Agudelo ML, Perez-Zapata LJ, Arboleda DM, Alzate JF, Cabarcas F, Zuluaga A, Pelham SJ, Ensser A, Schmidt M, Velasquez-Lopera MM, Jouanguy E, Puel A, Kronke M, Ghirardello S, Borghesi A, Pahari S, Boisson B, Pittaluga S, Ma CS, Emile JF, Notarangelo LD, Tangye SG, Marr N, Lachmann N, Salvator H, Schlesinger LS, Zhang P, Glickman MS, Nathan CF, Geissmann F, Abel L, Franco JL, Bustamante J, Casanova JL, Boisson-Dupuis S. Nature. 2024 Sep;633(8029):417-425. doi: 10.1038/s41586-024-07866-3. Epub 2024 Aug 28. PubMed
The ULK1 effector BAG2 regulates autophagy initiation by modulating AMBRA1 localization. Sankar DS, Kaeser-Pebernard S, Vionnet C, Favre S, de Oliveira Marchioro L, Pillet B, Zhou J, Stumpe M, Kovacs WJ, Kressler D, Antonioli M, Fimia GM, Dengjel J. Cell Rep. 2024 Sep 24;43(9):114689. doi: 10.1016/j.celrep.2024.114689. Epub 2024 Aug 27. PubMed
TRIP13 localizes to synapsed chromosomes and functions as a dosage-sensitive regulator of meiosis. Chotiner JY, Leu NA, Yang F, Cossu IG, Guan Y, Lin H, Wang PJ. Elife. 2024 Aug 29;12:RP92195. doi: 10.7554/eLife.92195. PubMed
FAM72A degrades UNG2 through the GID/CTLH complex to promote mutagenic repair during antibody maturation. Barbulescu P, Chana CK, Wong MK, Ben Makhlouf I, Bruce JP, Feng Y, Keszei AFA, Wong C, Mohamad-Ramshan R, McGary LC, Kashem MA, Ceccarelli DF, Orlicky S, Fang Y, Kuang H, Mazhab-Jafari M, Pezo RC, Bhagwat AS, Pugh TJ, Gingras AC, Sicheri F, Martin A. Nat Commun. 2024 Aug 30;15(1):7541. doi: 10.1038/s41467-024-52009-x. PubMed
Distinct functions of PAXX and MRI during chromosomal end joining. Cisneros-Aguirre M, Lopezcolorado FW, Ping X, Chen R, Stark JM. bioRxiv [Preprint]. 2024 Aug 22:2024.08.21.607864. doi: 10.1101/2024.08.21.607864. PubMed
Hyper-recombination in ribosomal DNA is driven by long-range resection-independent RAD51 accumulation. Gal Z, Boukoura S, Oxe KC, Badawi S, Nieto B, Korsholm LM, Geisler SB, Dulina E, Rasmussen AV, Dahl C, Lv W, Xu H, Pan X, Arampatzis S, Stratou DE, Galanos P, Lin L, Guldberg P, Bartek J, Luo Y, Larsen DH. Nat Commun. 2024 Sep 6;15(1):7797. doi: 10.1038/s41467-024-52189-6. PubMed
The prolyl isomerase FKBP11 is a secretory translocon accessory factor. DiGuilio A, Cheng B, Zhong F, Jha R, Wan Y, Anghel SA, Hu H, Shishkova E, Ji Z, Coon JJ, Keenan RJ. Mol Biol Cell. 2024 Nov 1;35(11):ar135. doi: 10.1091/mbc.E24-07-0305. Epub 2024 Sep 11. PubMed
HSF1 is required for cellular adaptation to daily temperature fluctuations. Takii R, Fujimoto M, Pandey A, Jaiswal K, Shearwin-Whyatt L, Grutzner F, Nakai A. Sci Rep. 2024 Sep 12;14(1):21361. doi: 10.1038/s41598-024-72415-x. PubMed
CRISPRi with barcoded expression reporters dissects regulatory networks in human cells. Kim J, Muller RY, Bondra ER, Ingolia NT. bioRxiv [Preprint]. 2024 Sep 6:2024.09.06.611573. doi: 10.1101/2024.09.06.611573. PubMed
Activation of the NLRP1B inflammasome by caspase-8. Meade JJ, Stuart S, Neiman-Zenevich J, Krustev C, Girardin SE, Mogridge J. Commun Biol. 2024 Sep 17;7(1):1164. doi: 10.1038/s42003-024-06882-3. PubMed
Identification of genes supporting cold resistance of mammalian cells: lessons from a hibernator. Sone M, Mitsuhashi N, Sugiura Y, Matsuoka Y, Maeda R, Yamauchi A, Okahashi R, Yamashita J, Sone K, Enju S, Anegawa D, Yamaguchi Y. Cell Death Dis. 2024 Sep 19;15(9):685. doi: 10.1038/s41419-024-07059-w. PubMed
Interleukin-34-orchestrated tumor-associated macrophage reprogramming is required for tumor immune escape driven by p53 inactivation. Nian Z, Dou Y, Shen Y, Liu J, Du X, Jiang Y, Zhou Y, Fu B, Sun R, Zheng X, Tian Z, Wei H. Immunity. 2024 Oct 8;57(10):2344-2361.e7. doi: 10.1016/j.immuni.2024.08.015. Epub 2024 Sep 24. PubMed
Chromatin protein complexes involved in gene repression in lamina-associated domains. Manzo SG, Mazouzi A, Leemans C, van Schaik T, Neyazi N, van Ruiten MS, Rowland BD, Brummelkamp TR, van Steensel B. EMBO J. 2024 Nov;43(21):5260-5287. doi: 10.1038/s44318-024-00214-1. Epub 2024 Sep 25. PubMed
CTNND2 moderates the pace of synaptic maturation and links human evolution to synaptic neoteny. Assendorp N, Fossati M, Libe-Philippot B, Christopoulou E, Depp M, Rapone R, Dingli F, Loew D, Vanderhaeghen P, Charrier C. Cell Rep. 2024 Oct 22;43(10):114797. doi: 10.1016/j.celrep.2024.114797. Epub 2024 Sep 30. PubMed
Generation of a fluorescent hESC reporter line (Kle033-A-1) for the isolation of distinct midbrain progenitor cell types. Sif Asgrimsdottir E, Arenas E. Stem Cell Res. 2024 Dec;81:103523. doi: 10.1016/j.scr.2024.103523. Epub 2024 Aug 12. PubMed
Cdk8 and Hira mutations trigger X chromosome elimination in naive female hybrid mouse embryonic stem cells. Halter K, Chen J, Priklopil T, Monfort A, Wutz A. Chromosome Res. 2024 Oct 10;32(4):12. doi: 10.1007/s10577-024-09756-w. PubMed
HSF1/HSP25 system protects mitochondria function from heat stress and assists steroidogenesis in MA-10 Leydig cells. Oka S, Takii R, Fujimoto M, Nakai A, Shiraishi K. Mol Cell Endocrinol. 2025 Jan 1;595:112391. doi: 10.1016/j.mce.2024.112391. Epub 2024 Oct 22. PubMed
Glucose deprivation impairs hypoxia-inducible factor-1alpha synthesis. Hubert M, Stuart S, Ohh M. Discov Oncol. 2024 Oct 28;15(1):595. doi: 10.1007/s12672-024-01484-1. PubMed
A temperature-sensitive and less immunogenic Sendai virus for efficient gene editing. Stevens CS, Carmichael JC, Watkinson R, Kowdle S, Reis RA, Hamane K, Jang J, Park A, Pernet O, Khamaikawin W, Hong P, Thibault P, Gowlikar A, An DS, Lee B. J Virol. 2024 Dec 17;98(12):e0083224. doi: 10.1128/jvi.00832-24. Epub 2024 Nov 4. PubMed
The glucocorticoid receptor potentiates aldosterone-induced transcription by the mineralocorticoid receptor. Johnson TA, Fettweis G, Wagh K, Ceacero-Heras D, Krishnamurthy M, Sanchez de Medina F, Martinez-Augustin O, Upadhyaya A, Hager GL, Alvarez de la Rosa D. Proc Natl Acad Sci U S A. 2024 Nov 19;121(47):e2413737121. doi: 10.1073/pnas.2413737121. Epub 2024 Nov 14. PubMed
Adaptor protein 3BP2 regulates gene expression in addition to the ubiquitination and proteolytic activity of MALT1 in dectin-1-stimulated cells. Tsubokawa A, Chihara K, Chihara Y, Takeuchi K, Fujieda S, Sada K. J Biol Chem. 2024 Dec;300(12):107980. doi: 10.1016/j.jbc.2024.107980. Epub 2024 Nov 13. PubMed
Impaired pheromone detection and abnormal sexual behavior in female mice deficient for ancV1R. Kondo H, Iwata T, Sato K, Koshiishi R, Suzuki H, Murata K, Spehr M, Touhara K, Nikaido M, Hirota J. Curr Biol. 2025 Jan 6;35(1):21-35.e8. doi: 10.1016/j.cub.2024.10.077. Epub 2024 Nov 21. PubMed
OCIAD1 and prohibitins regulate the stability of the TIM23 protein translocase. Elancheliyan P, Maruszczak KK, Serwa RA, Stephan T, Gulgec AS, Borrero-Landazabal MA, Ngati S, Gosk A, Jakobs S, Wasilewski M, Chacinska A. Cell Rep. 2024 Dec 24;43(12):115038. doi: 10.1016/j.celrep.2024.115038. Epub 2024 Dec 3. PubMed
PNKP safeguards stalled replication forks from nuclease-dependent degradation during replication stress. Mashayekhi F, Ganje C, Caron MC, Heyza JR, Gao Y, Zeinali E, Fanta M, Li L, Ali J, Mersaoui SY, Schmidt JC, Godbout R, Masson JY, Weinfeld M, Ismail IH. Cell Rep. 2024 Dec 24;43(12):115066. doi: 10.1016/j.celrep.2024.115066. Epub 2024 Dec 12. PubMed
Cas12f1 gene drives propagate efficiently in herpesviruses and induce minimal resistance. Lin Z, Yao Q, Lai K, Jiao K, Zeng X, Lei G, Zhang T, Dai H. Genome Biol. 2024 Dec 18;25(1):311. doi: 10.1186/s13059-024-03455-9. PubMed
Protocol for generating splice isoform-specific mouse mutants using CRISPR-Cas9 and a minigene splicing reporter. Teng Y, Arbogast K, Junge H, Chen Z. STAR Protoc. 2025 Mar 21;6(1):103543. doi: 10.1016/j.xpro.2024.103543. Epub 2025 Jan 4. PubMed
Deep CRISPR mutagenesis characterizes the functional diversity of TP53 mutations. Funk JS, Klimovich M, Drangenstein D, Pielhoop O, Hunold P, Borowek A, Noeparast M, Pavlakis E, Neumann M, Balourdas DI, Kochhan K, Merle N, Bullwinkel I, Wanzel M, Elmshauser S, Teply-Szymanski J, Nist A, Procida T, Bartkuhn M, Humpert K, Mernberger M, Savai R, Soussi T, Joerger AC, Stiewe T. Nat Genet. 2025 Jan;57(1):140-153. doi: 10.1038/s41588-024-02039-4. Epub 2025 Jan 7. PubMed Associated Plasmids HR700_HA2TP53_Ex5 HR700_HA2TP53_Ex6 HR700_HA2TP53_Ex7 HR700_HA2TP53_Ex8 HR700_TP53Exon5mut More ...
Pervasive RNA-binding protein enrichment on TAD boundaries regulates TAD organization. Sun Q, Zhou Q, Qiao Y, Chen X, Sun H, Wang H. Nucleic Acids Res. 2025 Jan 7;53(1):gkae1271. doi: 10.1093/nar/gkae1271. PubMed
Optimizing approaches for targeted integration of transgenic cassettes by integrase-mediated cassette exchange in mouse and human stem cells. Rath P, Kramer P, Biggs D, Preece C, Hortin N, Diaz R, Perez-Alcantara M, Li X, Bolard A, Beer N, McCarthy M, Davies B. Stem Cells. 2025 Jan 17;43(1):sxae092. doi: 10.1093/stmcls/sxae092. PubMed
Cell cycle progression of under-replicated cells. Huang M, Yang C, Nie L, Zhang H, Zhu D, Wang C, Park JM, Srivastava M, Mosa E, Li S, Tang M, Feng X, Keast SJ, Stossi F, Chen J. Nucleic Acids Res. 2025 Jan 7;53(1):gkae1311. doi: 10.1093/nar/gkae1311. PubMed
Functional rescue of F508del-CFTR through revertant mutations introduced by CRISPR base editing. Carrozzo I, Maule G, Gentile C, Umbach A, Ciciani M, Guidone D, De Santis M, Petris G, Vicente Galietta LJ, Arosio D, Cereseto A. Mol Ther. 2025 Mar 5;33(3):970-985. doi: 10.1016/j.ymthe.2025.01.011. Epub 2025 Jan 10. PubMed
Reduction of elevated Gli3 does not alter the progression of autosomal recessive polycystic kidney disease. Russell LG, Kolatsi-Joannou M, Wilson L, Chandler JC, Tejedor NP, Stagg G, Price KL, Rowan CJ, Crompton T, Rosenblum ND, Winyard PJD, Long DA. Physiol Rep. 2025 Jan;13(2):e70191. doi: 10.14814/phy2.70191. PubMed
Exocyst complex component 1 (Exoc1) loss in dormant oocyte disrupts c-KIT and growth differentiation factor (GDF9) subcellular localization and causes female infertility in mice. Nguyen CLK, Kuba Y, Le HT, Shawki HH, Mikami N, Aoki M, Yasuhara N, Suzuki H, Mizuno-Iijima S, Ayabe S, Osawa Y, Fujiyama T, Dinh TTH, Ishida M, Daitoku Y, Tanimoto Y, Murata K, Kang W, Ema M, Hirao Y, Ogura A, Takahashi S, Sugiyama F, Mizuno S. Cell Death Discov. 2025 Jan 20;11(1):17. doi: 10.1038/s41420-025-02291-5. PubMed
Generation and propagation of high fecundity gene edited fine wool sheep by CRISPR/Cas9. Zhang X, Qiu M, Han B, Liao L, Peng X, Lin J, Zhang N, Hai L, Liang L, Ma Y, Li W, Liu M. Sci Rep. 2025 Jan 20;15(1):2557. doi: 10.1038/s41598-025-86592-w. PubMed
Recombinant Marek's disease virus expressing VP1 and VP2 proteins provides robust immune protection against chicken infectious anemia virus. Ge C, Lu H, Han J, Sun G, Li S, Lan X, Liu Y, Yu M, Hu X, Hu M, Qi X, Cui H, Duan Y, Wang S, Chen Y, Wang X, Zhang Y, Gao Y, Liu C. Front Microbiol. 2025 Jan 6;15:1515415. doi: 10.3389/fmicb.2024.1515415. eCollection 2024. PubMed
The role of KLF4 in human primordial germ cell development. Lee SM, Smela MP, Surani MA. Open Biol. 2025 Jan;15(1):240214. doi: 10.1098/rsob.240214. Epub 2025 Jan 22. PubMed
Rapid human oogonia-like cell specification via transcription factor-directed differentiation. Pierson Smela M, Kramme CC, Fortuna PRJ, Wolf B, Goel S, Adams J, Ma C, Velychko S, Widocki U, Srikar Kavirayuni V, Chen T, Vincoff S, Dong E, Kohman RE, Kobayashi M, Shioda T, Church GM, Chatterjee P. EMBO Rep. 2025 Feb;26(4):1114-1143. doi: 10.1038/s44319-025-00371-2. Epub 2025 Jan 23. PubMed
HDAC6 Facilitates PRV and VSV Infection by Inhibiting Type I Interferon Production. Zheng H, Yang X, Zhong H, Song C, Wu Z, Yang H. Viruses. 2025 Jan 13;17(1):90. doi: 10.3390/v17010090. PubMed
Betacoronaviruses Differentially Activate the Integrated Stress Response to Optimize Viral Replication in Lung-Derived Cell Lines. Renner DM, Parenti NA, Bracci N, Weiss SR. Viruses. 2025 Jan 16;17(1):120. doi: 10.3390/v17010120. PubMed
SAA3 deficiency exacerbates intestinal fibrosis in DSS-induced IBD mouse model. Zou X, Wu T, Lin J, Su T, Xiao H, Ni C, Hu L, Lin W, Chen W, Ye RD, Xiang L. Cell Death Discov. 2025 Jan 26;11(1):25. doi: 10.1038/s41420-025-02299-x. PubMed
The PST repeat region of MDC1 is a tunable multivalent chromatin tethering domain. Heyza JR, Mikhova M, Perez GI, Broadbent DG, Schmidt JC. bioRxiv [Preprint]. 2025 Jan 13:2025.01.10.632395. doi: 10.1101/2025.01.10.632395. PubMed
Targeting of retrovirus-derived Rtl8a/8b causes late-onset obesity, reduced social response and increased apathy-like behaviour. Fujioka Y, Shiura H, Ishii M, Ono R, Endo T, Kiyonari H, Hirate Y, Ito H, Kanai-Azuma M, Kohda T, Kaneko-Ishino T, Ishino F. Open Biol. 2025 Jan;15(1):240279. doi: 10.1098/rsob.240279. Epub 2025 Jan 29. PubMed
The O-glycosyltransferase C1GALT1 promotes EWSR1::FLI1 expression and is a therapeutic target for Ewing sarcoma. Banday S, Mishra AK, Rashid R, Ye T, Ali A, Li J, Yustein JT, Kelliher MA, Zhu LJ, Deibler SK, Malonia SK, Green MR. Nat Commun. 2025 Feb 2;16(1):1267. doi: 10.1038/s41467-025-56632-0. PubMed
ROS-induced cytosolic release of mitochondrial PGAM5 promotes colorectal cancer progression by interacting with MST3. Wang S, Wu X, Bi W, Xu J, Hou L, Li G, Pan Y, Zhang H, Li M, Du S, Zhang M, Liu D, Jin S, Shi X, Tian Y, Shuai J, Plikus MV, Song M, Zhou Z, Yu L, Lv C, Yu Z. Nat Commun. 2025 Feb 6;16(1):1406. doi: 10.1038/s41467-025-56444-2. PubMed
Pioneer factor GATA6 promotes colorectal cancer through 3D genome regulation. Lyu H, Chen X, Cheng Y, Zhang T, Wang P, Wong JH, Wang J, Stasiak L, Sun L, Yang G, Wang L, Yue F. Sci Adv. 2025 Feb 7;11(6):eads4985. doi: 10.1126/sciadv.ads4985. Epub 2025 Feb 7. PubMed
PICH impacts the spindle assembly checkpoint via its DNA translocase and SUMO-interaction activities. Lama B, Park H, Saraf A, Hassebroek V, Keifenheim D, Saito-Fujita T, Saitoh N, Aksenova V, Arnaoutov A, Dasso M, Clarke DJ, Azuma Y. Life Sci Alliance. 2025 Feb 7;8(4):e202403140. doi: 10.26508/lsa.202403140. Print 2025 Apr. PubMed
Mutations in the kinesin KIF12 promote MASH in humans and mice by disrupting lipogenic enzyme turnover. Etemad A, Tanaka Y, Wang S, Slae M, Sultan M, Elpeleg O, Hirokawa N. EMBO J. 2025 Mar;44(6):1608-1640. doi: 10.1038/s44318-025-00366-8. Epub 2025 Feb 7. PubMed
Transdermal microneedle-assisted ultrasound-enhanced CRISPRa system to enable sono-gene therapy for obesity. Li S, Yu J, Shen Y, Xiong B, Zhao D, Xu W, Zhang S, Guan X, Liu Y, Shan X, Zhu A, Lyu Q, Fang Y, Chen Z, Yin H, Sun L, Xu H. Nat Commun. 2025 Feb 10;16(1):1499. doi: 10.1038/s41467-025-56755-4. PubMed
Proteogenomic analysis reveals adaptive strategies for alleviating the consequences of aneuploidy in cancer. Bokenkamp JE, Keuper K, Redel S, Barthel K, Johnson L, Becker A, Wieland A, Raschle M, Storchova Z. EMBO J. 2025 Mar;44(6):1829-1865. doi: 10.1038/s44318-025-00372-w. Epub 2025 Feb 10. PubMed
Construction of a TAT-Cas9-EGFP Site-Specific Integration Eukaryotic Cell Line Using Efficient PEG10 Modification. Qi S, Wang Y, Liu Z, Wu S, Zhao Y, Li Y, Deng S, Yu K, Lian Z. Int J Mol Sci. 2025 Feb 4;26(3):1331. doi: 10.3390/ijms26031331. PubMed
Cooperative nutrient scavenging is an evolutionary advantage in cancer. Guzelsoy G, Elorza SD, Ros M, Schachtner LT, Hayashi M, Hobson-Gutierrez S, Rundstrom P, Brunner JS, Pillai R, Walkowicz WE, Finley LWS, Deforet M, Papagiannakopoulos T, Carmona-Fontaine C. Nature. 2025 Apr;640(8058):534-542. doi: 10.1038/s41586-025-08588-w. Epub 2025 Feb 19. PubMed
Genetically encoded biosensor for fluorescence lifetime imaging of PTEN dynamics in the intact brain. Kagan T, Gabay M, Meenakshisundaram A, Levi Y, Eid S, Malchenko N, Maman M, Nitzan A, Ravotto L, Zaidel-Bar R, Eickholt BJ, Gal M, Laviv T. Nat Methods. 2025 Apr;22(4):764-777. doi: 10.1038/s41592-025-02610-9. Epub 2025 Feb 20. PubMed Associated Plasmids CMV_G-PTEN CMV_R-PTEN CMV_G-PTEN_4A CMV_R-PTEN_4A CAG_G-PTEN More ...
Engineering Base Changes and Epitope-Tagged Alleles in Mice Using Cas9 RNA-Guided Nuclease. Gertsenstein M, Lintott LG, Nutter LMJ. Curr Protoc. 2025 Feb;5(2):e70109. doi: 10.1002/cpz1.70109. PubMed
Attenuated growth factor signaling during cell death initiation sensitizes membranes towards peroxidation. Gollowitzer A, Pein H, Rao Z, Waltl L, Bereuter L, Loeser K, Meyer T, Jafari V, Witt F, Winkler R, Su F, Grosse S, Thurmer M, Grander J, Hotze M, Harder S, Espada L, Magnutzki A, Gstir R, Weinigel C, Rummler S, Bonn G, Pachmayr J, Ermolaeva M, Harayama T, Schluter H, Kosan C, Heller R, Thedieck K, Schmitt M, Shimizu T, Popp J, Shindou H, Kwiatkowski M, Koeberle A. Nat Commun. 2025 Feb 25;16(1):1774. doi: 10.1038/s41467-025-56711-2. PubMed
Mutations in Filamin C Associated with Both Alleles Do Not Affect the Functioning of Mice Cardiac Muscles. Ilchuk LA, Kochegarova KK, Baikova IP, Safonova PD, Bruter AV, Kubekina MV, Okulova YD, Minkovskaya TE, Kuznetsova NA, Dolmatova DM, Ryabinina AY, Mozhaev AA, Belousov VV, Ershov BP, Timashev PS, Filatov MA, Silaeva YY. Int J Mol Sci. 2025 Feb 7;26(4):1409. doi: 10.3390/ijms26041409. PubMed
CTCF-mediated insulation and chromatin environment modulate Car5b escape from X inactivation. Fang H, Tronco AR, Bonora G, Nguyen T, Thakur J, Berletch JB, Filippova GN, Henikoff S, Shendure J, Noble WS, Duan Z, Disteche CM, Deng X. BMC Biol. 2025 Mar 3;23(1):68. doi: 10.1186/s12915-025-02137-7. PubMed
Inducible, virus-free direct lineage reprogramming enhances scalable generation of human inner ear hair cell-like cells. Rainey RN, Houman SD, Menendez L, Chang R, Tao L, Bugacov H, McMahon AP, Kalluri R, Oghalai JS, Groves AK, Segil N. bioRxiv [Preprint]. 2025 Feb 25:2025.02.20.639352. doi: 10.1101/2025.02.20.639352. PubMed
SRBD1, a highly conserved gene required for chromosome individualization. Raaijmakers JA, Janssen LME, Mazouzi A, Hondema ALH, Borza R, Fish A, Elbatsh AMO, Kazokaite-Adomaitiene J, Vaquero-Siguero N, Mayayo-Peralta I, Nahidiazar L, Friskes A, Hoekman L, Bleijerveld OB, Hoencamp C, Moser SC, Jonkers J, Jalink K, Zwart W, Celie PHN, Rowland BD, Perrakis A, Brummelkamp TR, Medema RH. Cell Rep. 2025 Apr 22;44(4):115443. doi: 10.1016/j.celrep.2025.115443. Epub 2025 Mar 18. PubMed
SERTM2: a neuroactive player in the world of micropeptides. Lisi M, Santini T, D'Andrea T, Salvatori B, Setti A, Paiardini A, Nutarelli S, Nicoletti C, Pellegrini F, Fucile S, Bozzoni I, Martone J. EMBO Rep. 2025 Apr;26(8):2044-2076. doi: 10.1038/s44319-025-00404-w. Epub 2025 Mar 19. PubMed
VDAC2 loss elicits tumour destruction and inflammation for cancer therapy. Yuan S, Sun R, Shi H, Chapman NM, Hu H, Guy C, Rankin S, Kc A, Palacios G, Meng X, Sun X, Zhou P, Yang X, Gottschalk S, Chi H. Nature. 2025 Apr;640(8060):1062-1071. doi: 10.1038/s41586-025-08732-6. Epub 2025 Mar 19. PubMed
Induction of a transcriptional adaptation response by RNA destabilization events. Xie L, Jakutis G, Dooley CM, Guenther S, Kontarakis Z, Howard SP, Juan T, Stainier DYR. EMBO Rep. 2025 May;26(9):2262-2279. doi: 10.1038/s44319-025-00427-3. Epub 2025 Mar 24. PubMed
Noncanonical phagocytosis-like SEAL establishes mammalian fertilization. Inoue N, Saito T, Wada I. Cell Rep. 2025 Apr 22;44(4):115463. doi: 10.1016/j.celrep.2025.115463. Epub 2025 Mar 25. PubMed
Retinal gene therapy for Stargardt disease with dual AAV intein vectors is both safe and effective in large animal models. Ferla R, Pugni E, Lupo M, Tiberi P, Fioretto F, Perota A, Duchi R, Lagutina I, Gesualdo C, Rossi S, Ventrella D, Elmi A, McClinton B, Toomes C, Xu T, Molday RS, Surace EM, Simonelli F, Bacci ML, Galli C, Memon MA, Shams N, Auricchio A, Trapani I. Sci Adv. 2025 Mar 28;11(13):eadt9354. doi: 10.1126/sciadv.adt9354. Epub 2025 Mar 26. PubMed
Systematic high-throughput evaluation reveals FrCas9's superior specificity and efficiency for therapeutic genome editing. Tian R, Tian X, Yang M, Song Y, Zhao T, Zhong C, Zhu W, Zhou P, Han Z, Hu Z. Sci Adv. 2025 Mar 28;11(13):eadu7334. doi: 10.1126/sciadv.adu7334. Epub 2025 Mar 26. PubMed
Replication-dependent histone labeling dissects the physical properties of euchromatin/heterochromatin in living human cells. Minami K, Nakazato K, Ide S, Kaizu K, Higashi K, Tamura S, Toyoda A, Takahashi K, Kurokawa K, Maeshima K. Sci Adv. 2025 Mar 28;11(13):eadu8400. doi: 10.1126/sciadv.adu8400. Epub 2025 Mar 28. PubMed Associated Plasmids pX330_H3.2 pGEM_H3.2-HaloTag pX330_mouseH3.1 pGEM-T_mouseH3.1-HaloTag pX330_CDC45
Molecular basis for the calcium-dependent activation of the ribonuclease EndoU. Malard F, Dias K, Baudy M, Thore S, Vialet B, Barthelemy P, Fribourg S, Karginov FV, Campagne S. Nat Commun. 2025 Apr 1;16(1):3110. doi: 10.1038/s41467-025-58462-6. PubMed
Structure of the nucleosome-bound human BCL7A. Martin F, Kazrani AA, Lafouge J, Diaz-Jimenez DM, Siebert S, Fabbro-Burtschell L, Maillard E, Lapouge K, Mertens HDT, Sauter C, Leitner A, Ochsenbein F, Blais A, Bergamin E. Nucleic Acids Res. 2025 Apr 10;53(7):gkaf273. doi: 10.1093/nar/gkaf273. PubMed
Post-cleavage target residence determines asymmetry in non-homologous end joining of Cas12a-induced DNA double strand breaks. Chen RD, Yang Y, Liu KM, Hu JZ, Feng YL, Yang CY, Jiang RR, Liu SC, Wang Y, Han PA, Tian RG, Wang YL, Xu SM, Xie AY. Genome Biol. 2025 Apr 14;26(1):96. doi: 10.1186/s13059-025-03567-w. PubMed
Genome Editing in Mouse Embryo Using the CRISPR/Cas12i3 System. He J, Liu J, Yue Y, Wang L, Liu Z, Xi G, An L, Tian J, Wang Y. Int J Mol Sci. 2025 Mar 26;26(7):3036. doi: 10.3390/ijms26073036. PubMed
Transcription Factor p73 Is a Predictor of Platinum Resistance and Promotes Aggressive Epithelial Ovarian Cancers. Shoqafi A, Ali R, Lashen A, Jeyapalan JN, Ibrahim A, Toss MS, Rakha EA, Algethami M, Alqahtani S, Mongan NP, Ramotar D, Madhusudan S. Int J Mol Sci. 2025 Mar 31;26(7):3239. doi: 10.3390/ijms26073239. PubMed
Implementing complex nucleic acid circuits in living cells. Sun J, Xiong X, Lai W, Wu Z, Wang H, Yang L, Xue N, Yao Q, Song G, Zhao Y, Li L, Wang F, Fan C, Pei H. Sci Adv. 2025 May 2;11(18):eadv6512. doi: 10.1126/sciadv.adv6512. Epub 2025 Apr 30. PubMed
Cancer-Specific RNA Modifications in Tumour-Derived Extracellular Vesicles Promote Tumour Growth. Monoe Y, Jingushi K, Taniguchi K, Hirosuna K, Arima J, Inomata Y, Takano Y, Hamamoto H, Komura K, Tanaka T, Hase H, Lee SW, Tsujikawa K. J Extracell Vesicles. 2025 May;14(5):e70083. doi: 10.1002/jev2.70083. PubMed
m(6)A/IGF2BP3-driven serine biosynthesis fuels AML stemness and metabolic vulnerability. Huang F, Wang Y, Zhang X, Gao W, Li J, Yang Y, Mo H, Prince E, Long Y, Hu J, Jiang C, Kang Y, Chen Z, Hu YC, Zeng C, Yang L, Chen CW, Chen J, Huang H, Weng H. Nat Commun. 2025 May 6;16(1):4214. doi: 10.1038/s41467-025-58966-1. PubMed
Basal-shift transformation leads to EGFR therapy-resistance in human lung adenocarcinoma. Shinozaki T, Togasaki K, Hamamoto J, Mitsuishi A, Fukushima T, Sugihara K, Ebisudani T, Okada M, Saito A, Shigematsu L, Takaoka H, Ito F, Ohgino K, Ishioka K, Watanabe K, Hishima T, Kurebayashi Y, Emoto K, Terai H, Ikemura S, Kawada I, Asakura K, Hishida T, Asamura H, Ohta Y, Takahashi S, Oda M, Saito M, Matano M, Soejima K, Fujii M, Fukunaga K, Yasuda H, Sato T. Nat Commun. 2025 May 11;16(1):4369. doi: 10.1038/s41467-025-59623-3. PubMed
HR eye & MMR eye: one-day assessment of DNA repair-defective tumors eligible for targeted therapy. Saito S, Kato S, Arai U, En A, Tsunezumi J, Mizushima T, Tateishi K, Adachi N. Nat Commun. 2025 May 12;16(1):4239. doi: 10.1038/s41467-025-59462-2. PubMed
ARV1 is a component of the enzyme initiating glycosylphosphatidylinositol biosynthesis. Lu T, Umeshita S, Imanishi K, Wang Y, Liu YS, Nagae M, Senoo Y, Ikeda K, Fujita M, Kinoshita T, Murakami Y. J Biol Chem. 2025 Jun;301(6):110236. doi: 10.1016/j.jbc.2025.110236. Epub 2025 May 14. PubMed
Chromatin modification abnormalities by CHD7 and KMT2C loss promote medulloblastoma progression. Wang W, Kumegawa K, Chapman OS, Shiraishi R, Xiao Z, Okonechnikov K, Sun Y, Pfister SM, Feng W, Uesaka N, Hoshino M, Takahashi S, Korshunov A, Chavez L, Maruyama R, Kawauchi D. Cell Rep. 2025 May 27;44(5):115673. doi: 10.1016/j.celrep.2025.115673. Epub 2025 May 19. PubMed
Novel drug-inducible CRISPRa/i systems for rapid and reversible manipulation of gene transcription. Sui M, Zhou M, Cui M, Liu H, Zhang X, Hu N, Li Y, Wang B, Yang G, Gui P, Zhu L, Wan F, Zhang B. Cell Mol Life Sci. 2025 Jun 23;82(1):249. doi: 10.1007/s00018-025-05786-7. PubMed
Synaptic connectivity of sensorimotor circuits for vocal imitation in the songbird. Trusel M, Zhao Z, Alam DH, Marks ES, Ikeda MZ, Roberts TF. Elife. 2025 Jun 23;14:RP104609. doi: 10.7554/eLife.104609. PubMed

If you have published an article using this material, please email us at to have your article added to this page.